FastQCFastQC Report
Thu 26 May 2016
SRR936704_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936704_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037805
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101170.9748459489017687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79400.7650762908253477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47900.4615510620974075No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24640.23742417891607767No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22380.21564744822004134No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18550.17874263469534257No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18110.17450291721469832No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT15490.14925732676177123No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA14760.14222325003252057No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13670.13172031354637914No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13390.1290223115132419No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11560.11138894108238061No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT10950.10551115093876016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA134400.027.863471
GTACATG97950.027.2671721
TACATGG99950.026.4825442
ACATGGG96700.025.8983143
GTATAGG3500.025.7984621
ATGGGAT23750.025.6126565
GAGTACT72850.025.3799712-13
GGTATCA95250.025.3458581
CATGGGA65100.024.0172424
GTACTTT77750.023.68533514-15
TATCAAC159700.023.5076662
ATCAACG159250.023.4248893
TCAACGC160450.023.3100534
CAACGCA161550.023.2101695
AACGCAG163900.022.9063746
CATGGGG27750.022.434514
AGTACTT74900.021.95661412-13
CATATAG3059.094947E-1221.8140561
CCCTATA1754.0329887E-621.7239722
ACTTTTT87400.021.31282216-17
Produced by FastQC (version 0.11.5)