FastQCFastQC Report
Thu 26 May 2016
SRR936704_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936704_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1037805
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120461.160719017541831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101150.9746532344708302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66120.6371139086822669No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32650.31460630850689675No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29460.283868356772226No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25480.24551818501548942No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21160.20389186793280048No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19760.19040185776711424No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA17630.16987777087217734No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17220.16592712503794066No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13320.12834781100495757No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.11225615602160328No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11020.10618565144704449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111100.025.3961321
GTACATG92900.025.3606931
GTATCAA151700.025.3000241
TACATGG97100.024.3603942
ACATGGG93750.024.268253
CATGGGG27100.023.6567464
GAGTACT97200.023.01261912-13
AGTACTT99200.022.381112-13
CATGGGA62900.022.0456394
GTCGGGA4100.022.0112132
CGTGAAC1100.002877924621.5899473
GTACTTT104500.021.38231814-15
GTATAGG4050.021.1111851
ATGGGGG13100.021.0255435
CATATAG2056.830851E-720.8527283
AGGTAAG3650.020.8209882
ATCAACG185200.020.7226163
TCAACGC186400.020.5852414
TATCAAC186900.020.5341282
CAACGCA187750.020.4372235
Produced by FastQC (version 0.11.5)