##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936704_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1037805 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91787667239992 31.0 31.0 34.0 30.0 34.0 2 32.04933874860884 33.0 31.0 34.0 30.0 34.0 3 32.10319375990672 33.0 31.0 34.0 30.0 34.0 4 35.83258222883875 37.0 35.0 37.0 35.0 37.0 5 35.69067792118943 37.0 35.0 37.0 33.0 37.0 6 35.80444110406098 37.0 35.0 37.0 35.0 37.0 7 35.74816656308266 37.0 35.0 37.0 33.0 37.0 8 35.72952915046661 37.0 35.0 37.0 33.0 37.0 9 37.33569215796802 39.0 37.0 39.0 34.0 39.0 10-11 37.23100437943544 39.0 37.0 39.0 33.0 39.0 12-13 37.11797206604324 39.0 37.0 39.0 33.0 39.0 14-15 38.16484647886645 40.0 37.5 41.0 33.0 41.0 16-17 38.09531077610919 40.0 37.0 41.0 33.0 41.0 18-19 38.3154080005396 40.0 38.0 41.0 33.5 41.0 20-21 38.372257312308186 40.0 38.0 41.0 34.0 41.0 22-23 38.27500975616807 40.0 38.0 41.0 34.0 41.0 24-25 38.12237944507879 40.0 37.0 41.0 33.0 41.0 26-27 37.8990874971695 40.0 37.0 41.0 33.0 41.0 28-29 37.76636603215441 40.0 37.0 41.0 33.0 41.0 30-31 37.60718343041323 39.0 36.5 41.0 33.0 41.0 32-33 37.327509985016455 39.0 35.5 41.0 32.5 41.0 34-35 37.09277369062589 39.0 35.0 41.0 32.0 41.0 36-37 36.82705421538728 39.0 35.0 41.0 31.0 41.0 38-39 36.603296380341206 39.0 35.0 41.0 31.0 41.0 40-41 36.32894956181556 39.0 35.0 40.5 30.5 41.0 42-43 35.93368262823941 39.0 35.0 40.0 30.0 41.0 44-45 35.477200919247835 38.0 35.0 40.0 28.0 41.0 46-47 34.840347175047334 38.0 34.5 40.0 25.0 41.0 48-49 34.25508115686473 38.0 34.0 40.0 22.5 41.0 50-51 33.896948366986095 38.0 33.0 40.0 20.0 41.0 52-53 33.52177480355172 38.0 33.0 40.0 18.0 41.0 54-55 33.31105939940548 38.0 33.0 40.0 17.5 41.0 56-57 33.01413849422579 37.0 33.0 40.0 14.0 41.0 58-59 32.65317135685413 37.0 32.0 40.0 11.0 41.0 60-61 32.22611955039723 36.0 31.5 39.0 8.0 41.0 62-63 31.89600454806057 36.0 31.0 39.0 7.0 40.0 64-65 31.46619210738048 35.0 31.0 39.0 3.5 40.0 66-67 31.050809159716902 35.0 30.5 38.0 2.0 40.0 68-69 30.70034351347315 35.0 30.0 38.0 2.0 40.0 70-71 30.35139742051734 35.0 29.5 37.0 2.0 39.5 72-73 29.908760798030457 34.0 29.0 37.0 2.0 39.0 74-75 29.412418999715747 34.0 29.0 36.0 2.0 39.0 76-77 27.948841063590944 32.5 26.5 34.5 2.0 36.5 78-79 28.675628851277455 34.0 28.5 35.0 2.0 37.0 80-81 28.451160863553365 34.0 28.0 35.0 2.0 37.0 82-83 28.14831206247802 34.0 27.5 35.0 2.0 36.5 84-85 27.77007530316389 33.5 27.0 35.0 2.0 36.0 86-87 27.37640259971767 33.0 26.0 35.0 2.0 35.5 88-89 26.912164616666907 33.0 25.5 35.0 2.0 35.0 90-91 26.43170007853113 33.0 25.0 35.0 2.0 35.0 92-93 25.83818491913221 32.0 23.5 34.5 2.0 35.0 94-95 25.331641300629695 32.0 20.5 34.0 2.0 35.0 96-97 24.645530711453503 32.0 16.5 34.0 2.0 35.0 98-99 23.832147176010906 31.0 2.0 34.0 2.0 35.0 100-101 20.815151208560376 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3165884805436967 1101 2 0.23208122129303632 1101 3 0.23261273117007164 1101 4 0.22820348518763467 1101 5 0.1741078607472275 1101 6 0.2231846564786082 1101 7 0.23479608742881908 1101 8 0.15521423427446024 1101 9 0.2462357635959691 1101 10-11 0.312674910041558 1101 12-13 0.2928365935818036 1101 14-15 0.3935652322228478 1101 16-17 0.4093079488195599 1101 18-19 0.42006533521522726 1101 20-21 0.24582403361417704 1101 22-23 0.21056031368045325 1101 24-25 0.18485300522630155 1101 26-27 0.38391802054863433 1101 28-29 0.2117627886800264 1101 30-31 0.25472867758827533 1101 32-33 0.16923457955003585 1101 34-35 0.23912955193735996 1101 36-37 0.18447923894349572 1101 38-39 0.21465674815615188 1101 40-41 0.42297761304751447 1101 42-43 0.6173270740777852 1101 44-45 0.5288395135144981 1101 46-47 0.46666241483610094 1101 48-49 0.24710483328592403 1101 50-51 0.1438971577330932 1101 52-53 0.24137137637952577 1101 54-55 0.29189524098192976 1101 56-57 0.3879283654986452 1101 58-59 0.30140790442465715 1101 60-61 0.5139452825582751 1101 62-63 0.3054983309628767 1101 64-65 0.2196400462025423 1101 66-67 0.2335514994351655 1101 68-69 0.17017132470264684 1101 70-71 0.15974694398543576 1101 72-73 0.2249834518423235 1101 74-75 0.2029641203655963 1101 76-77 -0.01106938178682526 1101 78-79 0.17010813710501793 1101 80-81 0.15539027529397842 1101 82-83 0.09994525663209686 1101 84-85 0.29695359638830965 1101 86-87 0.2476877253509997 1101 88-89 0.0953814376348241 1101 90-91 0.354269778188371 1101 92-93 0.45829774142011814 1101 94-95 0.4689895223884264 1101 96-97 0.45147480683882435 1101 98-99 0.3822785374673465 1101 100-101 0.8911719843426091 1102 1 0.23879270963975685 1102 2 0.2354458799316177 1102 3 0.25186977252940324 1102 4 0.22890922179752238 1102 5 0.1780473242291336 1102 6 0.11051067729675879 1102 7 0.19339503119794443 1102 8 0.21324722188558098 1102 9 0.2629274056170132 1102 10-11 0.2829569960579832 1102 12-13 0.3042168661932436 1102 14-15 0.35787265713312166 1102 16-17 0.506769383299357 1102 18-19 0.2652565063854908 1102 20-21 0.1684311296842793 1102 22-23 0.2144353903870453 1102 24-25 0.21942043040994008 1102 26-27 0.36822095636976115 1102 28-29 0.4240452674917421 1102 30-31 0.29969186921554325 1102 32-33 0.3991302585859131 1102 34-35 0.47681243375993176 1102 36-37 0.5947003337776664 1102 38-39 0.5395998971514686 1102 40-41 0.4772099824017104 1102 42-43 0.5714505974084645 1102 44-45 0.58637035621868 1102 46-47 0.580732759726196 1102 48-49 0.9261169746893572 1102 50-51 0.7706793944649277 1102 52-53 0.9347629911351092 1102 54-55 0.9176983670857624 1102 56-57 0.7683707566004614 1102 58-59 0.8531597642672395 1102 60-61 0.9343322938392689 1102 62-63 0.8225201800215629 1102 64-65 0.558459084832446 1102 66-67 0.34433991729742175 1102 68-69 0.4555007799617563 1102 70-71 0.46483846313205035 1102 72-73 0.48904728611436 1102 74-75 0.3850567553098472 1102 76-77 0.451071913732644 1102 78-79 0.446286037405514 1102 80-81 0.5898488544541003 1102 82-83 0.4902672408670483 1102 84-85 0.5688380857939883 1102 86-87 0.5506987243387975 1102 88-89 0.45302263711368695 1102 90-91 0.5000914361730153 1102 92-93 0.3813836017245542 1102 94-95 0.5635186608214298 1102 96-97 0.4717767844120857 1102 98-99 0.5757651137239499 1102 100-101 1.3345806849743163 1103 1 0.025230924211452788 1103 2 0.22761892542125395 1103 3 0.17886539794540823 1103 4 0.09149581857818134 1103 5 0.17849082050361886 1103 6 0.0482975661955507 1103 7 0.02683881930304466 1103 8 0.04666944615237867 1103 9 0.15152286657280456 1103 10-11 0.16239968584491038 1103 12-13 0.18681793949735237 1103 14-15 0.15796401592807996 1103 16-17 0.24337033297079813 1103 18-19 0.28279257909299815 1103 20-21 0.03521261181001023 1103 22-23 0.012359224034973693 1103 24-25 0.13197187813417344 1103 26-27 0.05602365874997872 1103 28-29 -0.09973856796754177 1103 30-31 0.16826890027753905 1103 32-33 0.1531069897667905 1103 34-35 0.18367613161317564 1103 36-37 0.12505035800104025 1103 38-39 0.14902811041495312 1103 40-41 0.3794819484055978 1103 42-43 0.3846912254049428 1103 44-45 0.4010686996846218 1103 46-47 0.40654294907107413 1103 48-49 0.338767256672476 1103 50-51 0.2452829432976742 1103 52-53 0.5089637993477609 1103 54-55 0.355047510066953 1103 56-57 0.23688899026333132 1103 58-59 0.2428592937442815 1103 60-61 0.34182666208349843 1103 62-63 0.6270396730125967 1103 64-65 0.5339747840255278 1103 66-67 0.1424736389715271 1103 68-69 0.11891370302629056 1103 70-71 -0.14759323341011665 1103 72-73 0.07756396718088254 1103 74-75 -0.024210879237720917 1103 76-77 -0.088682100375852 1103 78-79 -0.22194065316347178 1103 80-81 -0.003863633452180437 1103 82-83 0.011441492494039807 1103 84-85 0.49508508662347595 1103 86-87 0.28023356359475926 1103 88-89 -0.11856714967745319 1103 90-91 0.038632124979514515 1103 92-93 0.16344723229862979 1103 94-95 -0.0720463620578009 1103 96-97 -0.012982613468043525 1103 98-99 -0.10689641020687901 1103 100-101 0.2585670767547583 1104 1 0.037853040051025744 1104 2 0.10405578700938634 1104 3 0.09599362305604586 1104 4 0.14947127016325368 1104 5 0.11204032090719807 1104 6 0.0336297491118529 1104 7 0.136026383666092 1104 8 0.03578687218705312 1104 9 0.11792883389725262 1104 10-11 0.06542370543911602 1104 12-13 0.0650708828022104 1104 14-15 0.17414175535862597 1104 16-17 0.07414074127606796 1104 18-19 -0.03486864164790404 1104 20-21 0.02348114141005908 1104 22-23 -0.02053899189088071 1104 24-25 0.16223990674615152 1104 26-27 0.17483436876139535 1104 28-29 0.3140706112470113 1104 30-31 0.2674923687848789 1104 32-33 0.10018316778455016 1104 34-35 -0.0030323768340423385 1104 36-37 0.08105386491745747 1104 38-39 -0.05360068526541539 1104 40-41 0.21571451819601606 1104 42-43 0.3181989768035365 1104 44-45 0.19212884608447212 1104 46-47 0.06525874967783096 1104 48-49 0.13096158437434013 1104 50-51 -0.14754736091341414 1104 52-53 -0.03820561811445344 1104 54-55 -0.20040908488419973 1104 56-57 -0.24137369447601031 1104 58-59 -0.18438610690699875 1104 60-61 -0.139977729911827 1104 62-63 -0.3329508638796028 1104 64-65 -0.3443407827867908 1104 66-67 -0.542363122418994 1104 68-69 -0.5236452326524095 1104 70-71 -0.5112270668037517 1104 72-73 -0.34457413852760155 1104 74-75 -0.4040085357269092 1104 76-77 -0.5285844077041979 1104 78-79 -0.3708732702694313 1104 80-81 -0.1760852371706214 1104 82-83 -0.2964017539085866 1104 84-85 -0.1669636762143405 1104 86-87 -0.07811206250115177 1104 88-89 -0.10430290630858607 1104 90-91 -0.10924980883439517 1104 92-93 0.17961801356167229 1104 94-95 0.26504206233475003 1104 96-97 0.5721436076903466 1104 98-99 0.2972617906893298 1104 100-101 0.9858695343795212 1105 1 0.00331046610315866 1105 2 0.0669600989688135 1105 3 0.10030994164139173 1105 4 0.13343633075555772 1105 5 0.2527396802612003 1105 6 0.08337000338608647 1105 7 0.0860471272483565 1105 8 0.25461062088306363 1105 9 0.09343261734628072 1105 10-11 0.04281620708507461 1105 12-13 0.03613734343600328 1105 14-15 0.24862511990753688 1105 16-17 0.20047592148969784 1105 18-19 0.12652168943770903 1105 20-21 0.17083047235436766 1105 22-23 0.11992061374055396 1105 24-25 0.028951492788436894 1105 26-27 -0.04550080543960178 1105 28-29 0.24443643484239175 1105 30-31 0.2944838278403452 1105 32-33 0.25194426785579793 1105 34-35 0.30263107207211704 1105 36-37 0.18422612984607412 1105 38-39 0.02215284790778327 1105 40-41 0.01043309692993688 1105 42-43 0.07055517718052329 1105 44-45 0.3819811006713536 1105 46-47 0.356297597296674 1105 48-49 0.2093246000703104 1105 50-51 0.204836873362801 1105 52-53 0.46045469219750856 1105 54-55 0.30242850188560055 1105 56-57 0.16911925120286497 1105 58-59 0.23097030691845077 1105 60-61 0.26605008452630585 1105 62-63 0.13085083348656212 1105 64-65 0.1597068462818818 1105 66-67 0.05141027047948299 1105 68-69 0.14801354744457385 1105 70-71 0.3107531636604506 1105 72-73 0.3940988133442822 1105 74-75 0.2388982113486975 1105 76-77 0.055486099431295344 1105 78-79 0.15893285239428678 1105 80-81 0.0812892566408756 1105 82-83 0.12256336681004143 1105 84-85 0.37572714219513514 1105 86-87 0.6141242008882273 1105 88-89 0.20506056634746272 1105 90-91 0.22780070930421203 1105 92-93 0.037725522437824566 1105 94-95 0.21974551929783104 1105 96-97 0.2200239292459365 1105 98-99 0.08889400998206654 1105 100-101 0.17431558453088414 1106 1 0.1674050744767861 1106 2 0.23941050839211897 1106 3 0.2459023074137292 1106 4 0.1300192569592724 1106 5 0.23669585413942684 1106 6 0.01768242871090564 1106 7 0.12109832861956704 1106 8 0.1090229467452346 1106 9 0.05684881537078468 1106 10-11 0.0770604473217773 1106 12-13 0.21255687564876524 1106 14-15 0.18477588401841416 1106 16-17 0.19152897449265538 1106 18-19 0.20288429648516626 1106 20-21 0.11235550378832215 1106 22-23 0.15606809370727603 1106 24-25 0.06727667310357788 1106 26-27 0.20200793663934746 1106 28-29 -0.028127981299327587 1106 30-31 0.1694736626322637 1106 32-33 0.23107744146504672 1106 34-35 0.3054851497280424 1106 36-37 -0.20110875902474135 1106 38-39 0.05403172241594234 1106 40-41 0.008693282468449581 1106 42-43 0.1800172656459793 1106 44-45 0.27469514867615885 1106 46-47 0.23794251563095514 1106 48-49 -0.16235215882345955 1106 50-51 0.14188217578556106 1106 52-53 -0.26904521084204447 1106 54-55 -0.2976761215638106 1106 56-57 -0.3753246024431647 1106 58-59 -0.5270242382744996 1106 60-61 -0.307768092742414 1106 62-63 -0.23073969316505227 1106 64-65 -0.23012497425997225 1106 66-67 -0.09566543373485459 1106 68-69 -0.48015799747938814 1106 70-71 -0.25771811415312484 1106 72-73 -0.2524944344553397 1106 74-75 -0.31448164214719654 1106 76-77 -0.49899550623369393 1106 78-79 -0.33167918696540255 1106 80-81 -0.16250058315544536 1106 82-83 -0.17768638453333097 1106 84-85 -0.09858537696237235 1106 86-87 0.16747512263802022 1106 88-89 0.19186299849078736 1106 90-91 0.3508880863051971 1106 92-93 0.23840158696969382 1106 94-95 0.08024367704934576 1106 96-97 0.4534882634404447 1106 98-99 0.4115689923102437 1106 100-101 0.6745118512409256 1107 1 -2.127920203776057E-5 1107 2 0.05950025895486277 1107 3 -0.01561533385035574 1107 4 0.11865443552181887 1107 5 0.021774221430050034 1107 6 -0.18344129839219647 1107 7 0.0064425462542558876 1107 8 -0.032261991236389065 1107 9 -0.04584289930777885 1107 10-11 0.14502917099947155 1107 12-13 -0.02028358366105465 1107 14-15 0.04478730740073189 1107 16-17 -0.03392559429872932 1107 18-19 -0.08974361090200489 1107 20-21 -0.06578027081165772 1107 22-23 -0.14789254696477627 1107 24-25 -0.0058975511252157276 1107 26-27 0.029185559003508388 1107 28-29 -0.04067805887244447 1107 30-31 -0.005970317068779707 1107 32-33 0.06548251909430292 1107 34-35 0.02536680862135654 1107 36-37 -0.06179523691680089 1107 38-39 -0.1383271258119052 1107 40-41 -0.133503360148147 1107 42-43 -0.10330587969890814 1107 44-45 0.08452591575948531 1107 46-47 -0.03950167906069879 1107 48-49 -0.06633879089910266 1107 50-51 -0.1778923086781603 1107 52-53 -0.16880262440859184 1107 54-55 -0.43403472592170544 1107 56-57 -0.32823403523693884 1107 58-59 -0.31049980234411834 1107 60-61 -0.2248101907941802 1107 62-63 -0.3602663287382981 1107 64-65 -0.32826578669990525 1107 66-67 -0.20540851994128317 1107 68-69 -0.2148816302433154 1107 70-71 -0.13497130411658986 1107 72-73 -0.1161346267917267 1107 74-75 0.27580118282957855 1107 76-77 -0.0393880726922724 1107 78-79 -0.021275646358645872 1107 80-81 0.24820891798609068 1107 82-83 0.3335745515101678 1107 84-85 0.4813718816184007 1107 86-87 0.48793629356679347 1107 88-89 0.3517025095956967 1107 90-91 0.26501778340907833 1107 92-93 0.4532630543929983 1107 94-95 0.6372073677003058 1107 96-97 0.8684442304730773 1107 98-99 0.6569783809828031 1107 100-101 0.8291502739156975 1108 1 0.05236796406730093 1108 2 0.26093729736148674 1108 3 0.07151089749331163 1108 4 0.1779515296021259 1108 5 0.1408743005236559 1108 6 0.1582995827311393 1108 7 0.21228366054965164 1108 8 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-0.3774914618738734 2316 54-55 -0.14846527025740386 2316 56-57 -0.07607150802765972 2316 58-59 -0.6658542765734836 2316 60-61 -0.3936503810257719 2316 62-63 -0.5178403503040272 2316 64-65 -0.37699197958993125 2316 66-67 -0.05676836682910036 2316 68-69 -0.5300345010431116 2316 70-71 -0.514232928337325 2316 72-73 -0.3661418682942248 2316 74-75 -0.30474363563400075 2316 76-77 -0.036691015317042996 2316 78-79 -0.1291531136994699 2316 80-81 -0.28124844136516103 2316 82-83 -0.5530471212876336 2316 84-85 -0.3821821190703467 2316 86-87 -0.48534363502662714 2316 88-89 -0.55550219534258 2316 90-91 -0.38165160820815913 2316 92-93 -0.3527928800451221 2316 94-95 -0.8100578654707107 2316 96-97 -1.0245043698785885 2316 98-99 -1.3658098227705473 2316 100-101 -2.693759694361159 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 20.0 5 46.0 6 115.0 7 268.0 8 548.0 9 1051.0 10 1879.0 11 3090.0 12 4447.0 13 6128.0 14 8068.0 15 11295.0 16 15195.0 17 20262.0 18 16356.0 19 9212.0 20 7363.0 21 7560.0 22 8089.0 23 8765.0 24 10071.0 25 11811.0 26 14110.0 27 16929.0 28 20560.0 29 25119.0 30 30534.0 31 37857.0 32 46694.0 33 59086.0 34 78213.0 35 105936.0 36 145953.0 37 186189.0 38 112792.0 39 6189.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.1296251140888 19.78451553583424 15.15957011051795 19.926289239559008 2 15.94863516997133 22.019225672778088 39.27154455874913 22.760594598501456 3 19.508453277620593 27.849271083083423 30.5458974813627 22.096378157933284 4 12.447714165956032 17.885440906528682 37.11217425238845 32.55467067512683 5 12.356641375549007 39.7291531362078 32.8375323526521 15.076673135591099 6 29.426626389350602 37.48960546538126 17.226261195503973 15.857506949764167 7 27.241341099724902 35.653904153477775 20.21102230187752 16.893732444919806 8 27.668010849822462 32.955902120340525 19.29427975390367 20.08180727593334 9 28.14796614007448 16.452705469717337 20.484387722163603 34.91494066804457 10-11 25.00373384209943 27.642765259369533 25.84551047643825 21.50799042209278 12-13 27.382970174148745 25.65858636276476 27.135087893419772 19.823355569666727 14-15 23.796134719209718 25.903182010458632 27.685023072779707 22.615660197551943 16-17 20.574770790273703 29.57260757078642 28.140402098660154 21.712219540279726 18-19 20.41535478120521 26.469050744974282 33.027281596665986 20.088312877154525 20-21 21.891636675483355 24.999253231580113 33.283034866858415 19.826075226078117 22-23 21.434036259220182 25.181079297170474 33.30100548754342 20.083878956065927 24-25 21.207982231729467 25.58390063643941 33.54979981788486 19.65831731394626 26-27 20.802607070966946 25.685565425860457 33.643703513128344 19.868123990044257 28-29 20.339580806905513 25.80509636199212 33.88561828403817 19.969704547064186 30-31 20.363205026843136 25.7165116013523 33.61330367411086 20.306979697693702 32-33 20.232367200501873 25.948409528279072 33.393931451049475 20.425291820169587 34-35 20.89129122415834 25.550534719809903 33.38634680639997 20.17182724963179 36-37 21.20908885929632 25.332771888170914 33.154332538702135 20.30380671383063 38-39 20.85272139488049 25.660848240741185 33.18911731122235 20.29731305315597 40-41 20.670926948563455 26.019870823268 33.11110104711587 20.198101181052667 42-43 20.758848770842107 26.28834884937793 32.243917810093635 20.70888456968633 44-45 20.748013684869314 26.377291712135953 31.912330884375766 20.962363718618967 46-47 21.5060840004471 26.74984871902594 30.9485702502592 20.795497030267757 48-49 21.551575862897977 27.126512760449668 30.082535448905446 21.239375927746913 50-51 21.543942043034118 27.524981824611476 29.77222588647476 21.15885024587964 52-53 21.81630021911694 27.70918730162401 29.35001088839682 21.124501590862227 54-55 21.04238714731579 27.69160649880371 29.516492241694138 21.749514112186365 56-57 21.78557901645411 27.922067283274206 28.808765888630926 21.483587811640756 58-59 22.021303461065422 26.97940401972464 29.13216011042098 21.867132408788954 60-61 21.597733509003415 27.53546572650405 29.6609997966365 21.205800967856035 62-63 21.93392510110825 27.52317671452754 29.65766550648572 20.88523267787848 64-65 21.698978785413864 27.65582292779022 29.46887396538896 21.176324321406955 66-67 21.77312659656169 27.806359169648196 29.068720929448233 21.351793304341875 68-69 21.737662014798744 27.7822798617448 29.218310815954275 21.261747307502183 70-71 21.725468657406736 27.99615534710278 29.015084722081703 21.26329127340878 72-73 21.736308844147022 27.92913890374396 28.875029509397237 21.45952274271178 74-75 21.500293170924188 28.671490055073733 28.505078818894564 21.32313795510751 76-77 21.606718533493417 28.131377759962074 28.97297708776183 21.28892661878268 78-79 21.512255692027043 28.154194631115015 28.7461760438816 21.58737363297634 80-81 21.457886496819402 28.57259194973875 28.441763519058306 21.52775803438354 82-83 21.53128008720048 28.34964808516503 28.426652043303402 21.69241978433109 84-85 21.228825730484584 28.304795741916333 28.300410039114688 22.165968488484395 86-87 21.32176960005088 28.3491998174756 28.134873386950275 22.194157195523236 88-89 21.4416235629483 28.14367938256484 27.934824574975885 22.479872479510973 90-91 21.14003569028698 28.037410207884832 28.45162770653753 22.370926395290656 92-93 21.212010220890367 28.305186687396322 28.317042303343765 22.165760788369546 94-95 20.879469361179375 27.927696942503687 28.355373402418294 22.837460293898644 96-97 21.17503744421932 27.798048513824835 28.33101702211559 22.695897019840256 98-99 20.94837880195395 28.097664774839092 27.97724214830495 22.976714274902005 100-101 20.698576099648918 28.310999815829234 28.123695256204673 22.86672882831717 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1838.0 1 1327.0 2 1258.0 3 1543.5 4 1167.0 5 1024.0 6 1533.5 7 2793.5 8 4141.5 9 4751.0 10 4190.5 11 3262.5 12 2953.5 13 2980.5 14 3091.5 15 3153.0 16 3241.5 17 3317.5 18 3374.0 19 3513.0 20 3681.5 21 3957.0 22 4421.0 23 4955.0 24 5468.0 25 6174.5 26 7215.0 27 8467.0 28 9565.0 29 10841.5 30 12910.5 31 15118.5 32 17841.0 33 20250.0 34 22381.5 35 24492.0 36 27341.0 37 29874.5 38 30100.5 39 29748.5 40 29291.0 41 29949.5 42 30557.0 43 31126.5 44 32696.5 45 34452.0 46 34905.0 47 36262.5 48 43860.0 49 42906.5 50 34284.0 51 30452.5 52 28075.5 53 27990.5 54 27979.5 55 26358.5 56 24417.0 57 22385.0 58 21121.5 59 19654.0 60 16844.0 61 14155.5 62 11915.5 63 9089.5 64 6184.5 65 4670.0 66 3656.0 67 2944.0 68 2378.0 69 1714.0 70 1315.0 71 1106.0 72 958.5 73 882.0 74 742.5 75 622.0 76 490.0 77 338.5 78 227.5 79 155.5 80 122.0 81 93.0 82 55.5 83 35.5 84 23.0 85 13.0 86 6.5 87 1.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02274030285072822 2 0.012526438010994359 3 0.009732078762387924 4 0.0 5 0.0022162159557913095 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001349001016568623 14-15 1.9271443093837472E-4 16-17 0.0 18-19 0.001686251270710779 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0011562865856302484 28-29 0.0024571089944642782 30-31 0.0015898940552415916 32-33 0.009057578254103613 34-35 0.0017826084861799663 36-37 6.745005082843115E-4 38-39 9.635721546918738E-4 40-41 1.9271443093837472E-4 42-43 0.005974147359089617 44-45 0.0015898940552415916 46-47 9.635721546918736E-5 48-49 8.190363314880926E-4 50-51 4.817860773459368E-5 52-53 2.890716464075621E-4 54-55 0.001349001016568623 56-57 0.018500585370083974 58-59 0.030930666165609147 60-61 0.024474732729173596 62-63 0.01729612017671913 64-65 0.02090951575681366 66-67 0.002698002033137246 68-69 0.001349001016568623 70-71 0.0 72-73 0.0 74-75 0.0013971796243032169 76-77 0.0018307870939145601 78-79 0.009972971801060894 80-81 0.017970620685003446 82-83 0.018886014231960725 84-85 0.03305052490593127 86-87 0.013056402696074889 88-89 0.04639599924841372 90-91 0.05280375407711468 92-93 0.03102702338107833 94-95 0.03261691743631993 96-97 0.05738072181190108 98-99 0.06017508106050751 100-101 0.06976262399969166 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1037805.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.14449578551085 #Duplication Level Percentage of deduplicated Percentage of total 1 72.37690833248259 31.950421248515827 2 14.091831796205026 12.44153618675401 3 5.286625232752786 7.001262159204918 4 2.508857931126804 4.430090734682906 5 1.4231869216239392 3.141293453181107 6 0.8853975882998397 2.3451258063122244 7 0.636006928670039 1.9653343627561166 8 0.4208410584744099 1.4862253065754811 9 0.30642392913827493 1.2174236863581829 >10 1.8012195704244314 14.977621751393269 >50 0.15797521185653018 4.82641924012789 >100 0.09618094994613262 7.775919355077679 >500 0.005696365999512706 1.7304537158935385 >1k 0.0021909099998125795 1.9280457649417444 >5k 2.1909099998125795E-4 0.6394902732778939 >10k+ 4.381819999625159E-4 2.1433369549472783 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12046 1.160719017541831 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10115 0.9746532344708302 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6612 0.6371139086822669 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3265 0.31460630850689675 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2946 0.283868356772226 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2548 0.24551818501548942 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2116 0.20389186793280048 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1976 0.19040185776711424 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1763 0.16987777087217734 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1722 0.16592712503794066 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1332 0.12834781100495757 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1165 0.11225615602160328 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1102 0.10618565144704449 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.635721546918737E-5 0.0 14-15 0.0 0.0 0.0 9.635721546918737E-5 0.0 16-17 0.0 0.0 0.0 1.4453582320378107E-4 0.0 18-19 0.0 0.0 0.0 2.890716464075621E-4 0.0 20-21 0.0 0.0 0.0 3.854288618767495E-4 0.0 22-23 0.0 0.0 0.0 6.263219005497179E-4 0.0 24-25 0.0 0.0 0.0 0.0010117507624264675 0.0 26-27 0.0 0.0 0.0 0.0017344298784453727 0.0 28-29 0.0 0.0 0.0 0.003950645834236682 0.0 30-31 4.8178607734593686E-5 0.0 0.0 0.008383077745819301 0.0 32-33 9.635721546918737E-5 0.0 0.0 0.012960045480605701 0.0 34-35 9.635721546918737E-5 0.0 0.0 0.01686251270710779 0.0 36-37 9.635721546918737E-5 0.0 0.0 0.02023501524852935 0.0 38-39 9.635721546918737E-5 0.0 0.0 0.023173910320339563 0.0 40-41 9.635721546918737E-5 0.0 0.0 0.0282326641324719 0.0 42-43 9.635721546918737E-5 0.0 0.0 0.03218330996670858 0.0 44-45 9.635721546918737E-5 0.0 0.0 0.03844652897220576 0.0 46-47 9.635721546918737E-5 0.0 0.0 0.047359571403105594 0.0 48-49 9.635721546918737E-5 0.0 0.0 0.057477079027370266 0.0 50-51 9.635721546918737E-5 0.0 0.0 0.07058166033117974 0.0 52-53 9.635721546918737E-5 0.0 0.0 0.09447824976753821 0.0 54-55 9.635721546918737E-5 0.0 0.0 0.12160280592211446 0.0 56-57 9.635721546918737E-5 9.635721546918737E-5 0.0 0.1595675488169743 0.0 58-59 9.635721546918737E-5 9.635721546918737E-5 0.0 0.21800819999903642 0.0 60-61 9.635721546918737E-5 9.635721546918737E-5 0.0 0.3013571913798835 0.0 62-63 9.635721546918737E-5 9.635721546918737E-5 0.0 0.36186952269453315 0.0 64-65 9.635721546918737E-5 9.635721546918737E-5 0.0 0.418093957920804 0.0 66-67 9.635721546918737E-5 9.635721546918737E-5 0.0 0.5326145085059332 0.0 68-69 9.635721546918737E-5 9.635721546918737E-5 0.0 0.6722361137207857 0.0 70-71 9.635721546918737E-5 9.635721546918737E-5 0.0 0.7883465583611564 0.0 72-73 9.635721546918737E-5 9.635721546918737E-5 0.0 0.8830175225596331 0.0 74-75 9.635721546918737E-5 9.635721546918737E-5 0.0 1.0701914136085295 0.0 76-77 9.635721546918737E-5 9.635721546918737E-5 0.0 1.2114029128786235 0.0 78-79 9.635721546918737E-5 9.635721546918737E-5 0.0 1.322213710668189 0.0 80-81 9.635721546918737E-5 9.635721546918737E-5 0.0 1.4343253308665886 0.0 82-83 9.635721546918737E-5 9.635721546918737E-5 0.0 1.6316649081474845 0.0 84-85 9.635721546918737E-5 9.635721546918737E-5 0.0 1.8263546620029776 0.0 86-87 9.635721546918737E-5 9.635721546918737E-5 0.0 2.120340526399468 0.0 88-89 9.635721546918737E-5 9.635721546918737E-5 0.0 2.50726292511599 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11110 0.0 25.396132 1 GTACATG 9290 0.0 25.360693 1 GTATCAA 15170 0.0 25.300024 1 TACATGG 9710 0.0 24.360394 2 ACATGGG 9375 0.0 24.26825 3 CATGGGG 2710 0.0 23.656746 4 GAGTACT 9720 0.0 23.012619 12-13 AGTACTT 9920 0.0 22.3811 12-13 CATGGGA 6290 0.0 22.045639 4 GTCGGGA 410 0.0 22.011213 2 CGTGAAC 110 0.0028779246 21.589947 3 GTACTTT 10450 0.0 21.382318 14-15 GTATAGG 405 0.0 21.111185 1 ATGGGGG 1310 0.0 21.025543 5 CATATAG 205 6.830851E-7 20.852728 3 AGGTAAG 365 0.0 20.820988 2 ATCAACG 18520 0.0 20.722616 3 TCAACGC 18640 0.0 20.585241 4 TATCAAC 18690 0.0 20.534128 2 CAACGCA 18775 0.0 20.437223 5 >>END_MODULE