FastQCFastQC Report
Thu 26 May 2016
SRR936703_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936703_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391701
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35270.25343087344192466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34990.2514189470295703No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33110.2379102982609052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29190.20974332848794386No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23890.1716604356826646No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22220.15966073172326528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15750.11317086069493375No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14930.10727879048732451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG41400.024.6698821
TACATGG42950.023.6689822
TAAGGTG5250.023.526575
GAGTACT41950.023.49967812-13
ACATGGG42000.023.4126193
GTATCAA90600.021.9167941
GTATTAG3500.021.7160531
GTACTTT45450.021.32646814-15
AGAGTAC63850.020.72277510-11
AGTACTT44400.020.43742612-13
TCAACGC99100.019.702114
ATCAACG100400.019.4936183
CAACGCA99550.019.4698875
TATCAAC101850.019.2627352
AACGCAG101050.019.1815646
GTAAGGT6000.019.0022284
GGTATCA70350.018.7044351
CCTATAC3408.894858E-1018.1632443
ACGCAGA107650.018.0055477
CGCAGAG107500.017.9884178
Produced by FastQC (version 0.11.5)