##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936703_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1391701 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.518507926630793 31.0 31.0 34.0 30.0 34.0 2 31.603932166463917 33.0 31.0 34.0 30.0 34.0 3 31.62460183617027 33.0 31.0 34.0 28.0 34.0 4 35.2398726450581 37.0 35.0 37.0 33.0 37.0 5 35.23880129424352 37.0 35.0 37.0 33.0 37.0 6 35.22357244839229 37.0 35.0 37.0 32.0 37.0 7 35.145419885449535 37.0 35.0 37.0 32.0 37.0 8 35.09858080147963 37.0 35.0 37.0 32.0 37.0 9 36.63056432380231 39.0 37.0 39.0 32.0 39.0 10-11 36.517867702904574 39.0 37.0 39.0 32.0 39.0 12-13 36.42361433957437 39.0 37.0 39.0 32.0 39.0 14-15 37.56146542971515 40.0 37.0 41.0 32.0 41.0 16-17 37.460398462025964 40.0 37.0 41.0 32.0 41.0 18-19 37.4014637483195 40.0 37.0 41.0 32.0 41.0 20-21 37.25608984975939 40.0 37.0 41.0 31.0 41.0 22-23 37.36258542603619 40.0 37.0 41.0 32.0 41.0 24-25 37.400002586762525 40.0 37.0 41.0 32.0 41.0 26-27 37.30788581742774 40.0 37.0 41.0 31.0 41.0 28-29 37.168789847819326 40.0 37.0 41.0 31.0 41.0 30-31 36.96288462823552 39.0 36.0 41.0 30.5 41.0 32-33 36.770414406542784 39.0 36.0 41.0 30.0 41.0 34-35 36.57618267142152 39.0 35.5 41.0 30.0 41.0 36-37 36.373230313120416 39.0 35.0 41.0 30.0 41.0 38-39 36.16970886706268 39.0 35.0 40.0 29.5 41.0 40-41 35.95264284497891 39.0 35.0 40.0 29.0 41.0 42-43 35.722226972604034 38.0 35.0 40.0 28.0 41.0 44-45 35.46579222117394 38.0 35.0 40.0 27.0 41.0 46-47 35.32437427292213 38.0 35.0 40.0 26.5 41.0 48-49 35.174005767043354 38.0 34.5 40.0 26.0 41.0 50-51 34.20181023078952 37.5 33.5 39.5 24.5 40.5 52-53 34.30784881235265 38.0 33.5 39.5 24.0 40.5 54-55 34.744488579084155 38.0 34.0 40.0 25.0 41.0 56-57 34.61332714426447 38.0 34.0 40.0 24.5 41.0 58-59 34.36842899444636 38.0 34.0 40.0 24.0 41.0 60-61 34.05550833117171 37.5 33.5 40.0 23.0 41.0 62-63 33.68318122930141 37.0 33.0 40.0 22.0 41.0 64-65 33.32153134904696 36.5 33.0 39.5 22.0 41.0 66-67 32.84070069648581 36.0 32.0 39.0 20.0 41.0 68-69 32.443098769060306 35.0 32.0 39.0 19.5 40.0 70-71 32.03459291902499 35.0 31.0 38.0 18.0 40.0 72-73 31.640942271364324 35.0 31.0 37.0 17.5 39.5 74-75 31.209276992687364 35.0 31.0 37.0 14.0 39.0 76-77 30.74254778864138 35.0 30.0 36.0 9.5 39.0 78-79 30.260057296790045 34.0 30.0 36.0 7.0 38.5 80-81 29.802843786129348 34.0 29.0 35.0 4.5 37.0 82-83 29.396964937152447 34.0 29.0 35.0 2.0 37.0 84-85 29.02559206323772 34.0 29.0 35.0 2.0 36.0 86-87 28.680773025240335 34.0 29.0 35.0 2.0 36.0 88-89 28.27614767827285 34.0 28.0 35.0 2.0 36.0 90-91 27.871861484614872 34.0 27.0 35.0 2.0 35.0 92-93 27.40917158211426 33.0 26.5 35.0 2.0 35.0 94-95 26.907731976911705 33.0 25.0 35.0 2.0 35.0 96-97 26.32976515788952 33.0 25.0 35.0 2.0 35.0 98-99 25.565112764882688 33.0 22.0 35.0 2.0 35.0 100-101 22.686244746536794 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35407239814060176 1101 2 0.3247539163741813 1101 3 0.3957210690840576 1101 4 0.3846843383694889 1101 5 0.3501432781006244 1101 6 0.4076032223260384 1101 7 0.4346895114252902 1101 8 0.4215628765913664 1101 9 0.3870072967774405 1101 10-11 0.6033651492405525 1101 12-13 0.4899722290234294 1101 14-15 0.6580758999042047 1101 16-17 0.8124075881446089 1101 18-19 0.7028426461576913 1101 20-21 0.7692667950208616 1101 22-23 0.552407410656528 1101 24-25 0.7026214146551126 1101 26-27 0.5549479690572241 1101 28-29 0.5355536766639162 1101 30-31 0.5799473930430636 1101 32-33 0.4539280001277959 1101 34-35 0.4075144487697031 1101 36-37 0.3483390326777922 1101 38-39 0.23380385537249282 1101 40-41 0.29292947452144347 1101 42-43 0.40963784828890226 1101 44-45 0.3220057064145294 1101 46-47 0.5045257754976191 1101 48-49 0.6166830305567004 1101 50-51 0.5915921046403341 1101 52-53 0.8111838019109143 1101 54-55 0.7318152742036332 1101 56-57 0.6556034578814405 1101 58-59 0.6214591644613918 1101 60-61 0.9123063079901712 1101 62-63 0.8283043404469481 1101 64-65 0.6506943969456245 1101 66-67 0.9219519470822419 1101 68-69 0.7475874721554661 1101 70-71 0.6483305555783829 1101 72-73 0.5228194008244991 1101 74-75 0.4925067484701202 1101 76-77 0.6364352771624695 1101 78-79 0.5018520228443215 1101 80-81 0.4484206541573066 1101 82-83 0.4709941964012607 1101 84-85 0.4915040328122515 1101 86-87 0.6756455386879949 1101 88-89 0.3699887620769289 1101 90-91 0.3261153476598295 1101 92-93 0.23806724491720743 1101 94-95 0.4284742467534457 1101 96-97 0.3427317857356904 1101 98-99 0.05234560408682398 1101 100-101 0.24104359819024168 1102 1 0.33894688569154496 1102 2 0.3688245351601722 1102 3 0.4288682576773333 1102 4 0.3888981423344333 1102 5 0.4214286986995788 1102 6 0.5482219482063684 1102 7 0.5043432100751915 1102 8 0.48883075670533316 1102 9 0.5681140064036683 1102 10-11 0.5093048996583249 1102 12-13 0.5575881537159617 1102 14-15 0.6118516425796372 1102 16-17 0.4515112080486716 1102 18-19 0.5373512060105412 1102 20-21 0.4691883497402003 1102 22-23 0.5254503361545559 1102 24-25 0.45906760299591554 1102 26-27 0.3026442024815239 1102 28-29 0.24403222894273568 1102 30-31 0.4718526515818766 1102 32-33 0.4847147672204599 1102 34-35 0.4678023519664194 1102 36-37 0.2960319599791532 1102 38-39 0.17450568516125742 1102 40-41 0.3087381521924968 1102 42-43 0.40912449756336144 1102 44-45 0.3879219833594334 1102 46-47 0.43498538669756215 1102 48-49 0.6969292465487129 1102 50-51 0.8106694859505268 1102 52-53 0.9773358655327442 1102 54-55 0.8907359452864725 1102 56-57 0.9867531471058797 1102 58-59 0.8105128919647271 1102 60-61 0.7930415296206306 1102 62-63 0.6650589600023622 1102 64-65 0.7545837500275283 1102 66-67 0.9718444980867034 1102 68-69 0.9480985687062784 1102 70-71 0.8064227423082002 1102 72-73 0.3583717938563886 1102 74-75 0.5341889939888205 1102 76-77 0.707719946369842 1102 78-79 0.6432951755631571 1102 80-81 0.849624533742297 1102 82-83 0.7616199853624117 1102 84-85 0.4105832174622215 1102 86-87 0.5509627762118932 1102 88-89 0.49844877001162047 1102 90-91 0.5213615937075105 1102 92-93 0.521088800838772 1102 94-95 0.25356185901134864 1102 96-97 0.3446657173770866 1102 98-99 0.2695544248228252 1102 100-101 0.2649497223321511 1103 1 0.4182349398627494 1103 2 0.3199965467055108 1103 3 0.35268501040137323 1103 4 0.43920675040218526 1103 5 0.4537476536268201 1103 6 0.38147247004363294 1103 7 0.5240244648815846 1103 8 0.543956996380949 1103 9 0.3247118771078732 1103 10-11 0.6224718026914715 1103 12-13 0.23057720761750744 1103 14-15 0.5508896892603445 1103 16-17 0.6155029385509323 1103 18-19 0.7285078121207818 1103 20-21 0.6279791853789405 1103 22-23 0.528071382425793 1103 24-25 0.5670423500671404 1103 26-27 0.4453829243048091 1103 28-29 0.5540266610139994 1103 30-31 0.5240444929433465 1103 32-33 0.4964554815133013 1103 34-35 0.5597711765591882 1103 36-37 0.6535179527683042 1103 38-39 0.5478221981897136 1103 40-41 0.4739832908663786 1103 42-43 0.5681575256177354 1103 44-45 0.6177488532346942 1103 46-47 0.4673055900393166 1103 48-49 0.30217059004247915 1103 50-51 0.6146615410405545 1103 52-53 0.8015647198877147 1103 54-55 0.7237486441589098 1103 56-57 0.4794761389062927 1103 58-59 0.49439186566812765 1103 60-61 0.6003337684772774 1103 62-63 0.5240524133166602 1103 64-65 0.5396510895063926 1103 66-67 0.47604728554662046 1103 68-69 0.4131997651867465 1103 70-71 0.30037292557545925 1103 72-73 0.2906034846091323 1103 74-75 0.36149144487619367 1103 76-77 0.25162855743379 1103 78-79 0.24740171026408575 1103 80-81 0.24710286126634173 1103 82-83 0.12339989987098932 1103 84-85 0.05725007555244943 1103 86-87 0.039602828345682184 1103 88-89 0.13613515829850087 1103 90-91 0.04125070953041288 1103 92-93 -0.15611240363154977 1103 94-95 0.02670683988563738 1103 96-97 0.03653391976006404 1103 98-99 0.21803298205513855 1103 100-101 0.34051188338761307 1104 1 0.320552861765254 1104 2 0.43910739131746723 1104 3 0.30185919408219064 1104 4 0.3997464608450656 1104 5 0.4577743151290292 1104 6 0.4790645426042346 1104 7 0.5196477128772514 1104 8 0.462635441979117 1104 9 0.5502998925913118 1104 10-11 0.6300071283128759 1104 12-13 0.6430992744922435 1104 14-15 0.480911625782241 1104 16-17 0.3857963938448776 1104 18-19 0.23798167078849986 1104 20-21 0.2520238852321981 1104 22-23 0.323251989398301 1104 24-25 0.42447202781006865 1104 26-27 0.2872497136272969 1104 28-29 0.3141966659550022 1104 30-31 0.3393909956385528 1104 32-33 0.17836318921504102 1104 34-35 0.3491085620038703 1104 36-37 0.2576841538890946 1104 38-39 0.24054826106199556 1104 40-41 0.05575611440834649 1104 42-43 0.20410002635640012 1104 44-45 0.3094516462616852 1104 46-47 0.2846449394668866 1104 48-49 0.5419745145321713 1104 50-51 0.35455174670010337 1104 52-53 0.4032532858325979 1104 54-55 0.31831435813248277 1104 56-57 0.24670785412629215 1104 58-59 0.33385302424422747 1104 60-61 0.1786773810636575 1104 62-63 0.21673688742361463 1104 64-65 0.4053088251408852 1104 66-67 0.4620657013001974 1104 68-69 0.42376827740143597 1104 70-71 0.2501864589321272 1104 72-73 0.1546780538860837 1104 74-75 0.042613000767772746 1104 76-77 0.060590563924122876 1104 78-79 0.19656316902417004 1104 80-81 0.3078050782578998 1104 82-83 0.19187673306652187 1104 84-85 0.0577932124130065 1104 86-87 0.10075724556914167 1104 88-89 0.03026071416028131 1104 90-91 -0.07213485184342971 1104 92-93 -0.04141276141907824 1104 94-95 -0.03867690648000277 1104 96-97 0.25422317347139156 1104 98-99 0.25695106584347016 1104 100-101 0.26291035093472104 1105 1 0.34946167787862237 1105 2 0.3399850371615578 1105 3 0.24713352387651 1105 4 0.43911332877919307 1105 5 0.3099530915126465 1105 6 0.45771447938081167 1105 7 0.5383379031460862 1105 8 0.6314494603700282 1105 9 0.556713890032448 1105 10-11 0.6879491498163262 1105 12-13 0.4645024603541401 1105 14-15 0.5742886917665402 1105 16-17 0.5968372982764976 1105 18-19 0.49220155361940954 1105 20-21 0.4792905758414676 1105 22-23 0.1915031696935472 1105 24-25 0.34523886431954054 1105 26-27 0.24249482597045358 1105 28-29 0.2657044884505595 1105 30-31 0.3176275485030544 1105 32-33 0.059022433676453545 1105 34-35 0.07838699196802423 1105 36-37 0.302844943396245 1105 38-39 0.20498228050038847 1105 40-41 0.20793283322498723 1105 42-43 0.19205203641229218 1105 44-45 0.35805044314615486 1105 46-47 0.4979136277866374 1105 48-49 0.2900525599183652 1105 50-51 0.48488084518648833 1105 52-53 0.5648293447313861 1105 54-55 0.5476320689208691 1105 56-57 0.46474168052749576 1105 58-59 0.5080793790585574 1105 60-61 0.4885853142937364 1105 62-63 0.5596809290837328 1105 64-65 0.5217315765942772 1105 66-67 0.64252835458894 1105 68-69 0.6835239506747541 1105 70-71 0.4493449140360184 1105 72-73 0.38154258367591254 1105 74-75 0.5509367944723635 1105 76-77 0.4214335678222767 1105 78-79 0.6834658199186485 1105 80-81 0.8402790967984473 1105 82-83 0.46813689931793334 1105 84-85 0.35473192684515453 1105 86-87 0.11725612700090693 1105 88-89 0.22771512969902474 1105 90-91 0.055985167909213374 1105 92-93 -0.0022619380181794213 1105 94-95 0.05512196073584974 1105 96-97 0.2829758015455859 1105 98-99 0.39156551520113325 1105 100-101 0.5164802296505009 1106 1 0.34076353876940857 1106 2 0.3571143843244613 1106 3 0.4559422487087268 1106 4 0.40099557405069675 1106 5 0.23671864977988832 1106 6 0.40786065591122167 1106 7 0.4817891945839534 1106 8 0.5672322039128375 1106 9 0.6716938066482712 1106 10-11 0.6468074475588494 1106 12-13 0.5136241300714417 1106 14-15 0.5655308109449066 1106 16-17 0.4921030853417818 1106 18-19 0.48613840535827535 1106 20-21 0.32408796245932336 1106 22-23 0.566691928082804 1106 24-25 0.5750645964732968 1106 26-27 0.573543356578547 1106 28-29 0.5860776834881918 1106 30-31 0.6207315753486924 1106 32-33 0.6064916730500514 1106 34-35 0.6283977963202787 1106 36-37 0.5922878201503678 1106 38-39 0.5643051653895483 1106 40-41 0.3959196646262484 1106 42-43 0.3856889084887598 1106 44-45 0.4913094171140173 1106 46-47 0.40737506260461487 1106 48-49 0.49577759550916767 1106 50-51 0.2990561553349522 1106 52-53 0.3431710673264661 1106 54-55 0.3514282098604653 1106 56-57 0.5547298592950369 1106 58-59 0.5429030774518893 1106 60-61 0.7019655512268272 1106 62-63 0.6502836848291054 1106 64-65 0.580741770818392 1106 66-67 0.6473579000434881 1106 68-69 0.6801193086165966 1106 70-71 0.7167812319127691 1106 72-73 0.5387262068201046 1106 74-75 0.8636376283141374 1106 76-77 0.6229360083347828 1106 78-79 0.44758456610534836 1106 80-81 0.7602824016591327 1106 82-83 0.7218396143880987 1106 84-85 0.8149426752689877 1106 86-87 0.8480110334479285 1106 88-89 0.8432956411897727 1106 90-91 0.7165651709598535 1106 92-93 0.6509221681441133 1106 94-95 0.6787682389542731 1106 96-97 0.7792165225136536 1106 98-99 1.0417504602896024 1106 100-101 0.706188766359876 1107 1 0.2314988558578186 1107 2 0.2003638896873703 1107 3 0.25902071377172575 1107 4 0.30759507420690113 1107 5 0.12018280160744155 1107 6 0.1460798322102903 1107 7 0.29819552593873766 1107 8 0.4074898057039462 1107 9 0.3680237295890123 1107 10-11 0.4448998405610425 1107 12-13 0.333735683752586 1107 14-15 0.1130224802149229 1107 16-17 0.3037397483637534 1107 18-19 0.28916220000579074 1107 20-21 0.4429303814950387 1107 22-23 0.3189363438903712 1107 24-25 0.40307960480402016 1107 26-27 0.26424059962759117 1107 28-29 0.27889763558649605 1107 30-31 0.2679108422444543 1107 32-33 -0.07223828113093589 1107 34-35 0.10059691378356916 1107 36-37 0.11477460813328833 1107 38-39 0.07489202626636171 1107 40-41 0.141229073144423 1107 42-43 0.18758545123581882 1107 44-45 0.28691853665999645 1107 46-47 0.3219274811196584 1107 48-49 0.2627696813156888 1107 50-51 0.13761441837775124 1107 52-53 0.24556940638717606 1107 54-55 0.3882803003530242 1107 56-57 0.22909952242114429 1107 58-59 0.36943938121113007 1107 60-61 0.3839597847996643 1107 62-63 0.2868809721351582 1107 64-65 0.11224527232834447 1107 66-67 0.10658906478366958 1107 68-69 0.1434390394298788 1107 70-71 0.07536546340293171 1107 72-73 -0.09556808923320759 1107 74-75 0.04637219043014085 1107 76-77 -0.1252637729835513 1107 78-79 0.023685459055467817 1107 80-81 0.029172982727551755 1107 82-83 0.17018453264281064 1107 84-85 -0.03933658642006321 1107 86-87 -0.11201480483177662 1107 88-89 -0.16682521947876694 1107 90-91 -0.02137381945980721 1107 92-93 -4.543548174602563E-4 1107 94-95 -0.021667171854847567 1107 96-97 0.14355741858780036 1107 98-99 0.08313954442997229 1107 100-101 0.3239433092110673 1108 1 0.17769397198640746 1108 2 0.14484498096730647 1108 3 0.14728137119501383 1108 4 0.16781043195330625 1108 5 0.18004069564992164 1108 6 0.19970148190423487 1108 7 0.1553335965561473 1108 8 -0.030109009870329828 1108 9 0.2644618665895422 1108 10-11 0.20225486044456176 1108 12-13 0.015772556751407762 1108 14-15 0.22173060597010874 1108 16-17 0.19000053562155017 1108 18-19 0.21735481996135064 1108 20-21 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2316 72-73 -1.472546026381476 2316 74-75 -1.2588920159546362 2316 76-77 -1.3959869165664003 2316 78-79 -2.126078971444059 2316 80-81 -1.9932205627677426 2316 82-83 -1.9746673026079513 2316 84-85 -1.7890741231499803 2316 86-87 -1.5598224645757846 2316 88-89 -1.9370971453714922 2316 90-91 -2.0614659555223582 2316 92-93 -2.71603929430648 2316 94-95 -2.330159626658375 2316 96-97 -2.7029005723480033 2316 98-99 -3.8319229586827284 2316 100-101 -4.633087419410874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3469.0 3 1391.0 4 1392.0 5 1480.0 6 1617.0 7 1930.0 8 2411.0 9 2956.0 10 3733.0 11 4449.0 12 5704.0 13 6602.0 14 7780.0 15 8957.0 16 10527.0 17 12849.0 18 10728.0 19 8988.0 20 9076.0 21 9648.0 22 10502.0 23 12101.0 24 13874.0 25 15978.0 26 17930.0 27 21491.0 28 25778.0 29 30718.0 30 37046.0 31 46360.0 32 58248.0 33 74495.0 34 99958.0 35 138826.0 36 197796.0 37 256924.0 38 191526.0 39 26459.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.40398631178489 17.59562276061002 14.526194305392071 23.47419662221302 2 15.608642411803764 18.896329001491853 40.295827117270164 25.199201469434218 3 19.824666425683013 22.76691874131794 29.55115956016411 27.857255272834934 4 12.918881392280248 15.837360324957384 37.15578923765527 34.087969045107094 5 14.265989401134638 36.07517101146354 34.521638691249294 15.137200896152539 6 32.979000247292724 34.709402299247195 17.419040389227238 14.892557064232847 7 29.08475667549811 31.6095856696409 20.548368483040257 18.757289171820734 8 26.483588989464696 33.415197919676395 19.862116749393742 20.239096341465167 9 25.649288104794653 16.89421340755138 20.63777299858541 36.818725489068555 10-11 25.187418367251585 25.30196479287835 27.46914578629454 22.041471053575524 12-13 26.342442152564992 22.76317141873471 27.68105691915914 23.213329509541154 14-15 23.087853718491473 24.35908401555391 26.20420616837611 26.348856097578505 16-17 22.547994700018076 27.45310137706813 26.933740522976112 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22.621174663180657 25.52522763307234 28.27615395587351 23.57744374787349 44-45 22.728252574941692 25.39211544016521 28.46857256183525 23.411059423057853 46-47 23.30722364774415 25.701393913743907 28.058702601795083 22.93267983671686 48-49 23.13088334464449 25.70562411910381 27.866624368081045 23.29686816817065 50-51 22.734296771059643 26.085609979818898 28.199433980635195 22.98065926848626 52-53 23.117621845427426 26.04314086043874 27.617853764289237 23.221383529844598 54-55 22.660801169267074 25.77054714542041 28.018001769134425 23.550649916178095 56-57 22.95885684270809 25.846498087396654 27.67722254137866 23.517422528516597 58-59 23.298956033848416 25.64324497562546 27.63488634795806 23.422912642568065 60-61 22.859096321174537 25.721490845151894 28.32520279635861 23.094210037314962 62-63 22.6477587745582 25.932424800279374 28.230235957042282 23.18958046812015 64-65 22.429899155092922 26.14537776511533 27.967620697546835 23.457102382244916 66-67 22.51596137648726 25.955778147646686 27.656947546648038 23.871312929218014 68-69 22.648903271997458 25.720956511862948 28.07302366839235 23.55711654774724 70-71 22.82785918862651 25.679032137475698 27.73902535110825 23.754083322789544 72-73 22.77876433087146 25.704012033152974 28.065367387022107 23.451856248953458 74-75 22.61186509630741 26.399005515807815 27.566065347300388 23.423064040584386 76-77 22.72261883156671 25.899522571394172 27.636845197772598 23.741013399266517 78-79 22.72524008978114 25.962659730340242 27.670643838905836 23.641456340972784 80-81 23.07022151845256 25.936345040814924 27.424400792028514 23.569032648704 82-83 22.86229117871608 25.944018139181118 27.815879259997327 23.377811422105474 84-85 22.841732499914656 26.150960413810697 27.463792462220372 23.543514624054275 86-87 22.762294189494973 26.331742511873667 27.43169834834139 23.474264950289975 88-89 22.67745306465083 26.169844083077116 27.127596878499215 24.02510597377284 90-91 22.675506238101278 25.992322281592777 27.42086957834367 23.911301901962272 92-93 22.924705169545703 25.856281361166722 27.804137059640976 23.4148764096466 94-95 22.57264276400206 25.762395541273797 27.804229695832287 23.860731998891858 96-97 22.360389729392917 25.75262662000953 28.092634396199013 23.794349254398544 98-99 22.470473081320925 26.084696614213108 27.41027616210626 24.034554142359703 100-101 22.454552351039247 25.745032963769514 27.5121369302438 24.28827775494744 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 52.0 1 49.0 2 65.5 3 87.5 4 76.5 5 77.5 6 139.5 7 307.0 8 543.0 9 701.5 10 674.5 11 642.0 12 690.5 13 751.0 14 852.0 15 943.5 16 1068.5 17 1215.0 18 1347.5 19 1494.5 20 1683.5 21 2000.0 22 2412.5 23 2903.5 24 3597.5 25 4608.0 26 5930.5 27 7554.0 28 9269.5 29 11330.5 30 13911.5 31 16835.5 32 20400.5 33 22809.0 34 24104.0 35 26055.5 36 28724.0 37 31292.5 38 33278.5 39 34876.5 40 36699.0 41 39210.0 42 41247.0 43 43155.0 44 45602.0 45 48805.5 46 51591.5 47 55931.0 48 63082.0 49 62195.0 50 54845.0 51 51155.5 52 50459.5 53 50959.5 54 50576.0 55 47683.0 56 43839.0 57 40150.5 58 36916.5 59 32757.5 60 27095.5 61 22480.0 62 19028.0 63 15071.5 64 11005.5 65 8178.5 66 6297.0 67 5004.0 68 3805.0 69 2834.0 70 2157.0 71 1562.5 72 1152.5 73 993.5 74 794.5 75 609.5 76 459.5 77 304.0 78 212.5 79 143.5 80 99.5 81 64.0 82 37.5 83 30.0 84 24.5 85 17.5 86 13.5 87 10.0 88 5.0 89 4.5 90 4.5 91 2.5 92 2.0 93 2.5 94 2.5 95 1.0 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009987777547044947 2 0.0104189046354066 3 0.013077521680303456 4 0.01846661028482411 5 0.03017889618531567 6 0.04577132588106209 7 0.005892070207609249 8 0.02723286108151104 9 0.0348494396425669 10-11 0.040382237276541444 12-13 0.02960406006750013 14-15 0.029532205552773187 16-17 0.026981370279966745 18-19 0.02324493551416576 20-21 0.04990296047786126 22-23 0.011388940584220318 24-25 0.0029819623611680958 26-27 0.0010418904635406598 28-29 3.592725736347103E-5 30-31 0.002011926412354378 32-33 0.0016167265813561964 34-35 3.592725736347103E-5 36-37 1.7963628681735516E-4 38-39 0.0 40-41 9.341086914502468E-4 42-43 0.0030538168758950375 44-45 0.0013652357798118993 46-47 5.748361178155365E-4 48-49 5.389088604520654E-4 50-51 0.0037723620231644587 52-53 0.0036286529937105743 54-55 0.0043112708836165236 56-57 8.263269193598338E-4 58-59 0.006107633751790075 60-61 0.0024430535007160304 62-63 5.029816030885945E-4 64-65 6.826178899059497E-4 66-67 0.015592429695746429 68-69 0.04045409179126838 70-71 0.04864550647013978 72-73 0.015233157122111719 74-75 0.03068187778840426 76-77 0.028274751545051702 78-79 0.0548249947366568 80-81 0.0786447663686381 82-83 0.04878921549959366 84-85 0.017496574336010394 86-87 0.02022704589563419 88-89 0.02292159019789452 90-91 0.03826252909209665 92-93 0.025185007411793192 94-95 0.013113448937666927 96-97 0.019688137035182126 98-99 0.05205859591966952 100-101 0.07239342358739413 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1391701.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.62173892100667 #Duplication Level Percentage of deduplicated Percentage of total 1 82.8349780515183 55.1861028127557 2 10.59887792847399 14.122313564128284 3 2.9840162951943388 5.964010636634004 4 1.2261181377721115 3.26744489843858 5 0.6499989398021966 2.165202983321654 6 0.38796162309561255 1.5508006779147534 7 0.25109744735005696 1.1709984006760699 8 0.19306830643694817 1.0290037044290616 9 0.12909534978967555 0.7740501020643417 >10 0.6905349068098676 7.950435349070374 >50 0.03329938061292207 1.53850138147111 >100 0.01706541560110059 2.289225637319853 >500 0.0025921450285543396 1.1671902286114002 >1k 0.0012960725142771698 1.824719623164817 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3527 0.25343087344192466 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3499 0.2514189470295703 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3311 0.2379102982609052 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2919 0.20974332848794386 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2389 0.1716604356826646 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2222 0.15966073172326528 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1575 0.11317086069493375 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1493 0.10727879048732451 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 7.185451472694207E-5 14-15 0.0 0.0 0.0 0.0 7.185451472694207E-5 16-17 0.0 0.0 0.0 0.0 7.185451472694207E-5 18-19 0.0 0.0 0.0 0.0 7.185451472694207E-5 20-21 0.0 0.0 0.0 0.0 7.185451472694207E-5 22-23 0.0 0.0 0.0 0.0 7.185451472694207E-5 24-25 0.0 0.0 0.0 2.155635441808262E-4 7.185451472694207E-5 26-27 0.0 7.185451472694207E-5 0.0 8.622541767233048E-4 7.185451472694207E-5 28-29 0.0 7.185451472694207E-5 0.0 0.0016167265813561964 7.185451472694207E-5 30-31 0.0 7.185451472694207E-5 0.0 0.0025867625301699143 7.185451472694207E-5 32-33 0.0 7.185451472694207E-5 0.0 0.004311270883616524 7.185451472694207E-5 34-35 0.0 7.185451472694207E-5 0.0 0.006107633751790076 7.185451472694207E-5 36-37 0.0 7.185451472694207E-5 0.0 0.007760287590509743 7.185451472694207E-5 38-39 0.0 1.077817720904131E-4 0.0 0.010023704804408418 7.185451472694207E-5 40-41 0.0 1.4370902945388413E-4 0.0 0.01322123070975734 7.185451472694207E-5 42-43 0.0 1.4370902945388413E-4 0.0 0.019041446402639647 7.185451472694207E-5 44-45 0.0 1.4370902945388413E-4 0.0 0.02895736943495765 7.185451472694207E-5 46-47 0.0 1.4370902945388413E-4 0.0 0.03905292875409301 1.4370902945388413E-4 48-49 0.0 1.4370902945388413E-4 0.0 0.05899255659081944 1.4370902945388413E-4 50-51 0.0 1.7963628681735518E-4 0.0 0.07882440265545546 1.4370902945388413E-4 52-53 0.0 2.155635441808262E-4 0.0 0.09779399454336815 1.4370902945388413E-4 54-55 0.0 2.5149080154429724E-4 0.0 0.13088299857512498 1.4370902945388413E-4 56-57 0.0 2.8741805890776826E-4 0.0 0.16652283787968825 1.4370902945388413E-4 58-59 0.0 2.8741805890776826E-4 0.0 0.20967147397321695 1.4370902945388413E-4 60-61 0.0 2.8741805890776826E-4 0.0 0.2608318884587997 1.4370902945388413E-4 62-63 0.0 2.8741805890776826E-4 0.0 0.33146487643538375 2.155635441808262E-4 64-65 0.0 2.8741805890776826E-4 0.0 0.38700841631930993 2.155635441808262E-4 66-67 0.0 2.8741805890776826E-4 0.0 0.47406016091100023 2.155635441808262E-4 68-69 0.0 2.8741805890776826E-4 0.0 0.5805844789936918 2.155635441808262E-4 70-71 0.0 2.8741805890776826E-4 0.0 0.6503552127935526 2.155635441808262E-4 72-73 0.0 2.8741805890776826E-4 0.0 0.7554065133243419 2.155635441808262E-4 74-75 0.0 2.8741805890776826E-4 0.0 0.8733556992486173 2.8741805890776826E-4 76-77 0.0 2.8741805890776826E-4 0.0 0.9721197297407993 2.8741805890776826E-4 78-79 0.0 3.233453162712393E-4 0.0 1.0712789600639794 2.8741805890776826E-4 80-81 0.0 3.592725736347103E-4 0.0 1.214664644201592 2.8741805890776826E-4 82-83 0.0 3.592725736347103E-4 0.0 1.387546606634615 2.8741805890776826E-4 84-85 0.0 3.592725736347103E-4 0.0 1.6119482561268548 2.8741805890776826E-4 86-87 0.0 3.592725736347103E-4 0.0 1.9126594002591073 2.8741805890776826E-4 88-89 0.0 3.592725736347103E-4 0.0 2.267979975583836 2.8741805890776826E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4140 0.0 24.669882 1 TACATGG 4295 0.0 23.668982 2 TAAGGTG 525 0.0 23.52657 5 GAGTACT 4195 0.0 23.499678 12-13 ACATGGG 4200 0.0 23.412619 3 GTATCAA 9060 0.0 21.916794 1 GTATTAG 350 0.0 21.716053 1 GTACTTT 4545 0.0 21.326468 14-15 AGAGTAC 6385 0.0 20.722775 10-11 AGTACTT 4440 0.0 20.437426 12-13 TCAACGC 9910 0.0 19.70211 4 ATCAACG 10040 0.0 19.493618 3 CAACGCA 9955 0.0 19.469887 5 TATCAAC 10185 0.0 19.262735 2 AACGCAG 10105 0.0 19.181564 6 GTAAGGT 600 0.0 19.002228 4 GGTATCA 7035 0.0 18.704435 1 CCTATAC 340 8.894858E-10 18.163244 3 ACGCAGA 10765 0.0 18.005547 7 CGCAGAG 10750 0.0 17.988417 8 >>END_MODULE