FastQCFastQC Report
Thu 26 May 2016
SRR936703_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936703_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1391701
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT66250.4760361600659912No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31880.22907219294949133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29610.21276121810647544No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT18790.13501463317192414No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC18200.13077521680303456No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17250.12394903790397506No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16560.11899107638781606No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16150.11604504128401144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15550.11173377040039491No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15540.11166191588566797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA700.00839526627.1408029
TAAGGTG8950.025.475685
GTAAGGT9400.023.7507634
GAGTACT46900.021.82443212-13
AAGGTAA9900.021.597811
TATTCCG1553.9062135E-521.4523035
GTACTTT48100.021.18235214-15
AGGTAAG10050.020.3284192
GGGTATA2601.48520485E-820.102581
GTACATG49450.019.9861641
GGTAAGG10700.019.9779973
TACCGTG1456.145601E-419.6558045
AGAGTAC73950.019.33254610-11
TAAGGTA4450.019.2141024
ACATGGG49700.018.8292483
GTATAGG5300.018.8267731
TACATGG50500.018.6283762
ACTTTTT55850.018.37056516-17
CCTACAC5450.018.3039993
ACCGTCG1300.007564073418.2691618
Produced by FastQC (version 0.11.5)