##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936703_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1391701 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01872744217328 31.0 31.0 34.0 30.0 34.0 2 32.149208774011086 33.0 31.0 34.0 30.0 34.0 3 32.272206458140076 34.0 31.0 34.0 30.0 34.0 4 35.96772582616525 37.0 35.0 37.0 35.0 37.0 5 35.83814411285183 37.0 35.0 37.0 35.0 37.0 6 35.88493146157113 37.0 35.0 37.0 35.0 37.0 7 35.8211383048514 37.0 35.0 37.0 35.0 37.0 8 35.786370060810476 37.0 35.0 37.0 33.0 37.0 9 37.42532268066201 39.0 37.0 39.0 34.0 39.0 10-11 37.323896440399196 39.0 37.0 39.0 34.0 39.0 12-13 37.21225931432111 39.0 37.0 39.0 33.0 39.0 14-15 38.35210364870041 40.0 38.0 41.0 33.5 41.0 16-17 38.32470192950928 40.0 38.0 41.0 33.5 41.0 18-19 38.44558062399898 40.0 38.0 41.0 34.0 41.0 20-21 38.432981653386754 40.0 38.0 41.0 34.0 41.0 22-23 38.33491640805029 40.0 38.0 41.0 34.0 41.0 24-25 38.2163801707407 40.0 38.0 41.0 33.0 41.0 26-27 38.02491447516384 40.0 37.0 41.0 33.0 41.0 28-29 38.063559988819435 40.0 37.5 41.0 33.0 41.0 30-31 37.968902084571326 40.0 37.0 41.0 33.0 41.0 32-33 37.80646166094585 40.0 37.0 41.0 33.0 41.0 34-35 37.63740199942373 40.0 37.0 41.0 33.0 41.0 36-37 37.47329311396629 40.0 37.0 41.0 32.0 41.0 38-39 37.269093361289535 39.5 36.0 41.0 31.5 41.0 40-41 37.059142732526595 39.0 36.0 41.0 31.0 41.0 42-43 36.8196175040472 39.0 36.0 41.0 31.0 41.0 44-45 36.57229390508449 39.0 35.0 40.0 30.0 41.0 46-47 36.24926726358607 39.0 35.0 40.0 30.0 41.0 48-49 35.883654247571855 39.0 35.0 40.0 29.0 41.0 50-51 35.585069278530376 38.0 35.0 40.0 28.0 41.0 52-53 35.32774712384341 38.0 34.0 40.0 27.5 41.0 54-55 34.99315010911108 38.0 34.0 40.0 27.0 41.0 56-57 34.636946082527786 38.0 34.0 40.0 26.0 41.0 58-59 34.24316465965031 37.0 33.0 40.0 26.0 41.0 60-61 33.842841961024675 37.0 33.0 39.0 25.0 40.5 62-63 33.51685275788406 36.0 32.0 39.0 24.5 40.0 64-65 33.187367114056826 36.0 32.0 39.0 23.5 40.0 66-67 32.72262899861393 35.0 31.5 38.5 22.5 40.0 68-69 32.24979395717902 35.0 31.0 38.0 21.5 40.0 70-71 31.72096880005116 35.0 31.0 37.0 20.0 39.5 72-73 31.15027186155647 34.0 30.0 36.5 18.5 39.0 74-75 30.61415886027243 34.0 29.5 36.0 17.5 39.0 76-77 29.264261145174142 32.5 28.0 34.5 16.0 36.5 78-79 30.048798197313936 34.0 29.0 35.0 16.5 37.0 80-81 29.89173141357231 34.0 29.0 35.0 12.0 37.0 82-83 29.510222382537627 34.0 29.0 35.0 7.5 36.0 84-85 29.06916464096814 33.5 29.0 35.0 7.0 36.0 86-87 28.669829941919996 33.0 29.0 35.0 2.0 35.5 88-89 28.171718637839593 33.0 27.5 35.0 2.0 35.0 90-91 27.592389097945606 33.0 26.5 34.5 2.0 35.0 92-93 27.006556005923684 32.0 25.5 34.0 2.0 35.0 94-95 26.266214150884423 32.0 25.0 34.0 2.0 35.0 96-97 25.429861371084737 32.0 23.0 34.0 2.0 35.0 98-99 24.257835914467258 31.0 10.0 34.0 2.0 35.0 100-101 20.41438821988344 27.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20989779289266863 1101 2 0.3857112121632156 1101 3 0.2427244601136067 1101 4 0.17979929071206868 1101 5 0.17799193612381714 1101 6 0.13811295614668495 1101 7 0.20440293186905478 1101 8 0.08657563263327006 1101 9 0.16489892244268134 1101 10-11 0.2631072631437519 1101 12-13 0.3082040359368321 1101 14-15 0.3018247908625895 1101 16-17 0.23251398185053773 1101 18-19 0.23830594533306737 1101 20-21 0.1533944847099562 1101 22-23 0.25174916151244986 1101 24-25 0.21666990785755758 1101 26-27 0.17359601808055913 1101 28-29 0.26973327199977604 1101 30-31 0.3804481116712992 1101 32-33 0.3836110175004066 1101 34-35 0.32908785645211225 1101 36-37 0.3922121604579516 1101 38-39 0.4304672432843546 1101 40-41 0.3366782595611113 1101 42-43 0.3828258734320471 1101 44-45 0.512391655948413 1101 46-47 0.3969450713439926 1101 48-49 0.5901457620368191 1101 50-51 0.5081388558730353 1101 52-53 0.2423147253916227 1101 54-55 0.2524392414376919 1101 56-57 0.29199210865542113 1101 58-59 0.4022497984199944 1101 60-61 0.3176536233095959 1101 62-63 0.41879182044783647 1101 64-65 0.39110040465259743 1101 66-67 0.2609678282149659 1101 68-69 0.2071831412884535 1101 70-71 0.08631392829871132 1101 72-73 0.17255534042842768 1101 74-75 0.30768707772525516 1101 76-77 0.18499261475345463 1101 78-79 0.33340066525671475 1101 80-81 0.524523440297127 1101 82-83 0.3552560715810422 1101 84-85 0.2504341327634556 1101 86-87 0.20231756872085782 1101 88-89 0.036059919982662336 1101 90-91 0.28618207307676613 1101 92-93 0.2023182424219634 1101 94-95 -0.044459426899752685 1101 96-97 0.2211621398372543 1101 98-99 -0.5577954014511732 1101 100-101 -0.6120487547423039 1102 1 0.2524403817672649 1102 2 0.39526560690227086 1102 3 0.3419406084202734 1102 4 0.2197132112929836 1102 5 0.11131284644974215 1102 6 0.16935702015322818 1102 7 0.17861817850582895 1102 8 0.14829072899172502 1102 9 0.24727097548491628 1102 10-11 0.2752906267331241 1102 12-13 0.3278237716874415 1102 14-15 0.4750381319703223 1102 16-17 0.5463416907674556 1102 18-19 0.30885349940320594 1102 20-21 0.23540106088903912 1102 22-23 0.19832461926019107 1102 24-25 0.28350047807359857 1102 26-27 0.30413272205632325 1102 28-29 0.3857140183419929 1102 30-31 0.540661777380194 1102 32-33 0.41305700146271107 1102 34-35 0.42902549095120435 1102 36-37 0.48313730382346165 1102 38-39 0.49913842230790806 1102 40-41 0.5694305167414129 1102 42-43 0.61624626800279 1102 44-45 0.734643516393966 1102 46-47 0.7011910348684438 1102 48-49 0.7966951915803335 1102 50-51 0.5996739789209116 1102 52-53 0.6569775309074402 1102 54-55 0.6481330163087478 1102 56-57 0.709513508287543 1102 58-59 0.6001033732405574 1102 60-61 0.5762875633665843 1102 62-63 0.4871701070345864 1102 64-65 0.5242261034467575 1102 66-67 0.48522319916195755 1102 68-69 0.5612645500574942 1102 70-71 0.33478920451025473 1102 72-73 0.18074205221866535 1102 74-75 0.2887683903161786 1102 76-77 0.12657883242858858 1102 78-79 0.25822243517459853 1102 80-81 0.44214252083428107 1102 82-83 0.3743349556743816 1102 84-85 0.17254172602617857 1102 86-87 0.37416803578586055 1102 88-89 0.35808185460558306 1102 90-91 0.16427434997357793 1102 92-93 0.32593372830181266 1102 94-95 0.1943588144832873 1102 96-97 0.35844077987860956 1102 98-99 0.589301221494523 1102 100-101 0.3309599974667883 1103 1 0.3404885605801695 1103 2 0.341869669742465 1103 3 0.25788236239272777 1103 4 0.16181174615370963 1103 5 0.2599440847770609 1103 6 0.20814525908977544 1103 7 0.23168578264856166 1103 8 0.2585482020010019 1103 9 0.27029548435864115 1103 10-11 0.4360926581532141 1103 12-13 0.3409009137170642 1103 14-15 0.4631563468374438 1103 16-17 0.40441818362153725 1103 18-19 0.3377493017342488 1103 20-21 0.37230469327591464 1103 22-23 0.3926153700067516 1103 24-25 0.32879545345486605 1103 26-27 0.32948115454706084 1103 28-29 0.42309716674352416 1103 30-31 0.4796374033352322 1103 32-33 0.455936918967474 1103 34-35 0.5123477966230041 1103 36-37 0.42381202193146805 1103 38-39 0.23436718178336946 1103 40-41 0.3461291095285688 1103 42-43 0.4043491273713897 1103 44-45 0.3156362805834405 1103 46-47 0.25368062309139816 1103 48-49 0.23452290039013945 1103 50-51 0.3232912350056907 1103 52-53 0.378686585408218 1103 54-55 0.41060155626301054 1103 56-57 0.41770847567895686 1103 58-59 0.17170306662995927 1103 60-61 -0.0737662432989552 1103 62-63 0.2718953809367335 1103 64-65 0.06675646488773168 1103 66-67 0.0577105152705073 1103 68-69 0.29581978990289315 1103 70-71 0.07345484914157652 1103 72-73 0.27311661152834077 1103 74-75 0.2974529858798114 1103 76-77 0.21967185429082292 1103 78-79 0.04142027391939607 1103 80-81 0.3338269566008556 1103 82-83 0.05576017065136085 1103 84-85 0.2763542591091621 1103 86-87 0.22447314829297937 1103 88-89 0.13250271511273937 1103 90-91 0.18808099054005822 1103 92-93 0.23303858013997925 1103 94-95 0.33064760272222316 1103 96-97 0.8260107160596597 1103 98-99 0.36505101925137495 1103 100-101 0.11152493000246722 1104 1 0.21877934922730446 1104 2 0.24090890719072888 1104 3 0.2266634763757409 1104 4 0.16806371696460332 1104 5 0.07440356738799636 1104 6 0.21044940083422148 1104 7 0.12692244134508712 1104 8 0.1459785873736763 1104 9 0.21807737549291062 1104 10-11 0.2582432690735601 1104 12-13 0.28238831418335053 1104 14-15 0.3154600540723962 1104 16-17 0.26108778012361 1104 18-19 0.24827976333229884 1104 20-21 0.3505900815908305 1104 22-23 0.2768949408603447 1104 24-25 0.2134288391776309 1104 26-27 0.2066437703438453 1104 28-29 0.31628821684123665 1104 30-31 0.39787109743558347 1104 32-33 0.4503165387344339 1104 34-35 0.37451362778999453 1104 36-37 0.4320186647125297 1104 38-39 0.36697140819509855 1104 40-41 0.26989069541371435 1104 42-43 0.15760298271201378 1104 44-45 0.10168318774490359 1104 46-47 -0.009756893202705896 1104 48-49 0.21314751646997365 1104 50-51 -0.057555400525266975 1104 52-53 -0.012434647704878898 1104 54-55 -0.29580348603338535 1104 56-57 0.01972261337624559 1104 58-59 0.08486417281662995 1104 60-61 -0.14319247972674276 1104 62-63 -0.23618246081738903 1104 64-65 -0.047548084054177764 1104 66-67 0.22741347216965124 1104 68-69 0.06812129976129455 1104 70-71 0.04811301431973547 1104 72-73 0.09211547683784715 1104 74-75 0.103334320097062 1104 76-77 -0.02425028905758353 1104 78-79 0.25581780789182673 1104 80-81 0.14674362016388542 1104 82-83 0.17145483959237495 1104 84-85 0.3539089266100852 1104 86-87 0.3773025499361715 1104 88-89 0.09459238297369055 1104 90-91 0.23597479890399597 1104 92-93 0.280904145387165 1104 94-95 0.22421512425527368 1104 96-97 0.4641893888442219 1104 98-99 0.49558449758673007 1104 100-101 0.46626964947072835 1105 1 0.13557508145927955 1105 2 0.29852826478090577 1105 3 0.25003743730441386 1105 4 0.21039348135447966 1105 5 0.1674168181193636 1105 6 0.15475293311557436 1105 7 0.12666243969903235 1105 8 0.1753269746152597 1105 9 0.20222101611452103 1105 10-11 0.12694991987825688 1105 12-13 0.024968914693630495 1105 14-15 0.2344826349347784 1105 16-17 0.245839338262968 1105 18-19 0.29718251897564585 1105 20-21 0.11904739268747733 1105 22-23 0.12644657830956874 1105 24-25 0.1918430910088631 1105 26-27 0.34599062376277345 1105 28-29 0.18877892037949806 1105 30-31 0.30387956712949915 1105 32-33 0.2999906949449169 1105 34-35 0.0928299020225225 1105 36-37 0.2699478544800584 1105 38-39 0.3180181486348914 1105 40-41 0.3041342215597851 1105 42-43 0.29800106580778873 1105 44-45 0.17786928158492543 1105 46-47 0.3619425823047635 1105 48-49 0.3355353416590816 1105 50-51 0.4511125680201431 1105 52-53 0.30783313528539935 1105 54-55 0.4071549211853096 1105 56-57 0.4554900484506632 1105 58-59 0.4778239268064368 1105 60-61 0.4904380785098894 1105 62-63 0.2610080249928757 1105 64-65 0.16980487472714145 1105 66-67 0.1865071700290244 1105 68-69 0.3081439704084161 1105 70-71 0.30340283198189155 1105 72-73 0.36211876894502026 1105 74-75 0.2927015621342761 1105 76-77 -0.05515213937145447 1105 78-79 0.18153028722263187 1105 80-81 0.2862822014153359 1105 82-83 0.3721611462222292 1105 84-85 0.4231844011598547 1105 86-87 0.3445535656260539 1105 88-89 0.2222429033510842 1105 90-91 0.34758097310135483 1105 92-93 0.3384542939060893 1105 94-95 0.6230080806423359 1105 96-97 0.6110052104120136 1105 98-99 0.6568341515413749 1105 100-101 0.5772117906871621 1106 1 0.13978750773546267 1106 2 0.27356004228751374 1106 3 0.22145039235830666 1106 4 0.1373166560545016 1106 5 0.2312654554998801 1106 6 0.2237164779599965 1106 7 0.19588020459632816 1106 8 0.15962888635006323 1106 9 0.17414368237443512 1106 10-11 0.20932420806210672 1106 12-13 0.17244133181789323 1106 14-15 0.26832186617333775 1106 16-17 0.3038923939880007 1106 18-19 0.19675479373668026 1106 20-21 0.11991364751766298 1106 22-23 0.17651076887291595 1106 24-25 0.23878482377273968 1106 26-27 0.17370569392750213 1106 28-29 0.2858388654679658 1106 30-31 0.38509334077195945 1106 32-33 0.23236116982797483 1106 34-35 0.189938148921037 1106 36-37 0.23262390186668114 1106 38-39 0.1770124541057072 1106 40-41 0.13567975237125296 1106 42-43 0.24302725047323293 1106 44-45 0.4375264658436109 1106 46-47 0.4125304661770457 1106 48-49 0.4346627313275775 1106 50-51 0.34613239718427025 1106 52-53 0.4439781424035587 1106 54-55 0.33603813249301595 1106 56-57 0.3706860521114308 1106 58-59 0.3994294535503826 1106 60-61 0.5271360142430623 1106 62-63 0.5435717071161292 1106 64-65 0.645380047596781 1106 66-67 0.13358999492225365 1106 68-69 0.40983135600939136 1106 70-71 0.29854659342179346 1106 72-73 0.29391851980937034 1106 74-75 0.40058937482493917 1106 76-77 0.3516412559915736 1106 78-79 0.24103684609999831 1106 80-81 0.30184110420158916 1106 82-83 0.35832394874409346 1106 84-85 0.4649311471431794 1106 86-87 0.4061021470781583 1106 88-89 0.36165018929780146 1106 90-91 0.5872780699083506 1106 92-93 0.5922288261411914 1106 94-95 0.3039898784865507 1106 96-97 0.41752531921819624 1106 98-99 0.8145891342544118 1106 100-101 0.4884661124280605 1107 1 -0.05049547674855148 1107 2 0.06919486994554092 1107 3 0.08081074849701508 1107 4 0.07842164928577944 1107 5 -0.015251908865423047 1107 6 0.01189139725604349 1107 7 -0.05619271532452785 1107 8 -0.05173084498390068 1107 9 -0.08937357785674038 1107 10-11 -0.05448300062268174 1107 12-13 0.18867291309457812 1107 14-15 0.17067139236306872 1107 16-17 0.06915552790449198 1107 18-19 0.052406347938493525 1107 20-21 -0.007363310203885476 1107 22-23 0.0068414598028283535 1107 24-25 0.05205281294279729 1107 26-27 -0.016026681371883456 1107 28-29 0.0076439003528960825 1107 30-31 -0.1201442802839594 1107 32-33 -0.23597261909215206 1107 34-35 -0.2504600339384808 1107 36-37 -0.15719145531336665 1107 38-39 -0.14633181331072365 1107 40-41 -0.32557356690397654 1107 42-43 -0.13520676493861572 1107 44-45 -0.05538102400331013 1107 46-47 -0.006441144257195219 1107 48-49 0.005894052268949679 1107 50-51 -0.1162686753972153 1107 52-53 -0.153837644329748 1107 54-55 -0.06406404911690089 1107 56-57 0.13926292912903193 1107 58-59 -0.012274245814396068 1107 60-61 -0.18932563836549576 1107 62-63 -0.42328858393875635 1107 64-65 -0.09937126709654365 1107 66-67 -0.1137226938208471 1107 68-69 -0.15594268595665994 1107 70-71 -0.24990213353853008 1107 72-73 -0.16067542218792497 1107 74-75 -0.3315402331100792 1107 76-77 -0.49791584855908866 1107 78-79 -0.14372898228696585 1107 80-81 0.004681687873354434 1107 82-83 0.03480135163902176 1107 84-85 -0.19253071373363184 1107 86-87 0.11759953070827223 1107 88-89 -0.0036819334763329437 1107 90-91 -0.1039938633416746 1107 92-93 0.049917504158479176 1107 94-95 0.3289313030413794 1107 96-97 0.5034525685152893 1107 98-99 0.5468531402941892 1107 100-101 0.3980659770536974 1108 1 -0.14052313987408738 1108 2 0.07491072923006925 1108 3 -0.19429181028974796 1108 4 0.009918243224184664 1108 5 0.012457286977365811 1108 6 -0.02929755173365578 1108 7 -0.049101029130333984 1108 8 0.022645478228625393 1108 9 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-0.681929356156747 2316 90-91 -1.1444042423446135 2316 92-93 -1.0901995335426022 2316 94-95 -0.9424626906834099 2316 96-97 -0.963302249951763 2316 98-99 -0.20565296060724592 2316 100-101 -0.06492321563517933 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 13.0 5 26.0 6 98.0 7 179.0 8 460.0 9 911.0 10 1429.0 11 2219.0 12 3318.0 13 4530.0 14 5886.0 15 7629.0 16 9427.0 17 11925.0 18 11470.0 19 8921.0 20 8702.0 21 8952.0 22 9560.0 23 11011.0 24 12560.0 25 15248.0 26 18401.0 27 22777.0 28 28269.0 29 34767.0 30 42514.0 31 53182.0 32 67526.0 33 86042.0 34 115311.0 35 159448.0 36 226015.0 37 268588.0 38 129669.0 39 4708.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22757106093565 19.143639639328878 14.752532667585088 22.87625663215038 2 16.076701358672416 20.542922823641348 38.51969597126717 24.860679846419067 3 20.48380988789333 23.5145919758325 29.139902315357414 26.861695820916758 4 13.791948816408182 16.712198641082534 36.14992067233074 33.345931870178546 5 14.43060205415419 37.185393415870294 33.50234511026366 14.881659419711859 6 33.00859882977738 36.03719477100326 16.397703242291268 14.556503156928105 7 29.647846184467408 32.09041074432364 19.725974047437187 18.535769023771767 8 26.98161202009097 34.391055350765626 18.79123642817622 19.836096200967184 9 26.179761313672977 17.422492331326918 19.68777776260849 36.70996859239161 10-11 25.584015532071902 26.14933092668612 26.535441161571345 21.731212379670634 12-13 26.752621432333523 23.40017719323332 26.86712160155091 22.98007977288225 14-15 23.135620135431875 25.41690139225808 25.28213776571895 26.165340706591095 16-17 22.852643526268192 28.2768368585021 25.967970779704885 22.90254883552482 18-19 22.816840988850853 27.22483537834956 27.992291309184036 21.966032323615554 20-21 23.507485476343597 26.01791340775098 28.156097420771076 22.31850369513435 22-23 23.09585399152116 25.864841560681178 28.18362434432708 22.855680103470576 24-25 23.390719073818723 26.154978145323714 27.994320586508664 22.4599821943489 26-27 22.95698385821354 26.226082732718332 28.21893535129915 22.59799805776898 28-29 22.7298905188293 27.092790438747414 27.792833299980853 22.38448574244243 30-31 22.993126352669986 26.27632407170552 27.806260293225797 22.92428928239869 32-33 22.924339676534466 26.406184037265184 27.860144181626456 22.809332104573894 34-35 23.488955123497686 26.354867405434845 27.706501322855875 22.449676148211594 36-37 23.69682396143097 25.914492955130342 27.93998504691969 22.448698036518998 38-39 23.193052133787813 26.501351121695087 27.73864876333112 22.566947981185976 40-41 23.425135957870218 26.388527120976907 27.54491900011461 22.641417921038272 42-43 23.135128959428833 26.67330373836477 27.133359872039364 23.058207430167034 44-45 23.331115291696428 26.407570180897345 27.292475377582065 22.96883914982416 46-47 23.994988866142943 26.844092229580923 26.756393794356693 22.404525109919444 48-49 23.851708299920386 26.886897524998638 26.552265038701538 22.709129136379445 50-51 23.210784049551442 27.196240528567888 27.025907443135477 22.56706797874519 52-53 23.84350599682186 27.12389312173532 26.391692723013044 22.64090815842977 54-55 23.24877973070365 27.092780705050867 26.73049024179763 22.92794932244785 56-57 23.647619489068955 27.1813849402649 26.46357198988012 22.707423580786028 58-59 23.879090719325646 26.782038721063124 26.29722603358623 23.041644526025 60-61 23.268545987135468 27.14385851958333 27.114433133774845 22.473162359506357 62-63 23.09550301627614 27.117276394796434 27.119863160114665 22.667357428812757 64-65 23.042091894018462 27.544816163459757 26.6582474495341 22.754844492987676 66-67 23.247281525004652 26.994656102943132 26.612061241045897 23.146001131006315 68-69 23.245790050611728 27.025519994452722 26.589965246877227 23.13872470805832 70-71 23.56721744507276 27.07269462966802 26.339457178652616 23.020630746606603 72-73 23.495742875271166 26.909239063655317 26.622352904825654 22.972665156247867 74-75 23.255887484586044 27.734198718593618 26.123781614779002 22.886132182041337 76-77 23.57560617911264 27.01714500047963 26.199074002551576 23.20817481785615 78-79 23.52182005015773 27.39826531858782 26.19742600297497 22.882488628279475 80-81 23.776711192356515 27.383173507196577 26.12137876225552 22.718736538191386 82-83 23.612147054638896 27.412718464322104 26.217083942324415 22.758050538714585 84-85 23.306194407927475 27.88257818953032 26.008278563865694 22.802948838676507 86-87 23.37616845094428 27.763594413423075 25.766638188163626 23.09359894746902 88-89 23.093477852377447 27.773316648109564 25.61842933983784 23.51477615967515 90-91 23.459231174968274 27.532376913115904 25.83139428763605 23.176997624279775 92-93 23.53265799954371 27.602912354512515 26.021524692677616 22.84290495326616 94-95 23.19617444914295 27.51741581309123 26.05596053733038 23.23044920043544 96-97 23.12073498546024 27.60388186830361 26.319554272074246 22.955828874161906 98-99 23.10119624169716 27.828176061944742 25.558528754925657 23.512098941432445 100-101 22.827436597938885 27.910703225036897 25.582320284779335 23.67953989224488 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 94.0 1 70.0 2 59.0 3 81.5 4 82.0 5 90.0 6 141.5 7 254.0 8 417.5 9 539.5 10 548.5 11 562.0 12 656.0 13 755.5 14 871.0 15 975.0 16 1080.0 17 1230.0 18 1403.5 19 1529.5 20 1711.5 21 2043.0 22 2391.5 23 2900.0 24 3603.0 25 4549.0 26 5835.5 27 7529.0 28 9367.5 29 11559.5 30 14203.5 31 16770.5 32 20090.0 33 22543.0 34 24004.0 35 25967.5 36 28851.5 37 31813.0 38 33525.0 39 34876.0 40 36383.5 41 39203.5 42 41622.5 43 43204.0 44 45530.0 45 48623.0 46 50796.5 47 54658.0 48 62638.5 49 62135.0 50 54736.5 51 51173.0 52 49991.0 53 50391.5 54 50904.5 55 48700.5 56 43979.0 57 40122.0 58 37757.0 59 33445.0 60 27210.0 61 22725.0 62 19397.5 63 15102.0 64 10818.5 65 8068.0 66 6281.5 67 4957.5 68 3799.0 69 2838.0 70 2147.0 71 1590.5 72 1241.0 73 1023.0 74 830.0 75 634.0 76 463.5 77 310.5 78 216.5 79 163.0 80 127.0 81 86.0 82 56.0 83 37.0 84 23.0 85 14.5 86 5.5 87 4.0 88 3.5 89 2.5 90 1.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01322123070975734 2 0.008766250796686931 3 0.005748361178155365 4 3.592725736347103E-4 5 0.005892070207609249 6 0.0 7 5.748361178155365E-4 8 0.002227489956535204 9 0.0 10-11 0.0 12-13 0.0 14-15 3.592725736347103E-4 16-17 0.004706470714614705 18-19 0.0017963628681735516 20-21 4.311270883616524E-4 22-23 7.185451472694207E-5 24-25 5.748361178155365E-4 26-27 1.7963628681735516E-4 28-29 3.592725736347103E-5 30-31 6.466906325424786E-4 32-33 0.0038442165378914004 34-35 0.0010778177209041309 36-37 0.0010418904635406598 38-39 0.0057124339207918935 40-41 1.077817720904131E-4 42-43 0.0016526538387196675 44-45 0.0059639247223361915 46-47 0.0 48-49 0.0017963628681735516 50-51 0.001149672235631073 52-53 3.951998309981814E-4 54-55 0.0 56-57 0.013113448937666927 58-59 0.002119708184444791 60-61 0.003269380420075864 62-63 1.077817720904131E-4 64-65 0.00312567139062198 66-67 0.0014370902945388413 68-69 0.001975999154990907 70-71 0.004634616199887763 72-73 0.0024430535007160304 74-75 0.007544724046328917 76-77 8.981814340867758E-4 78-79 0.006538760840151728 80-81 0.002335271728625617 82-83 0.003951998309981814 84-85 0.002478980758079501 86-87 5.748361178155365E-4 88-89 0.0036286529937105743 90-91 0.008838105311413874 92-93 0.00312567139062198 94-95 4.311270883616524E-4 96-97 0.0 98-99 6.466906325424786E-4 100-101 0.0019400718976274357 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1391701.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.83796904685251 #Duplication Level Percentage of deduplicated Percentage of total 1 81.17990810750031 51.823604609929355 2 11.507758841541557 14.692639054499464 3 3.2309084678303455 6.187639042877019 4 1.4015581739930139 3.578905093149166 5 0.735731865369409 2.348381402411769 6 0.4477363438834851 1.7149547317190899 7 0.28320658675301585 1.2655533323302641 8 0.21154546309850508 1.0803706180227557 9 0.15402756231370796 0.884952607981017 >10 0.783803289762059 8.783197232778479 >50 0.039522992696725365 1.7341629334567197 >100 0.020018731612815532 2.4888310396272653 >500 0.002474174215572245 1.1239312059917812 >1k 0.001686936965162894 1.8172434804256763 >5k 1.1246246434419296E-4 0.47563361480009897 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6625 0.4760361600659912 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3188 0.22907219294949133 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2961 0.21276121810647544 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1879 0.13501463317192414 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1820 0.13077521680303456 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1725 0.12394903790397506 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1656 0.11899107638781606 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1615 0.11604504128401144 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1555 0.11173377040039491 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1554 0.11166191588566797 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.185451472694207E-5 2 0.0 0.0 0.0 0.0 7.185451472694207E-5 3 0.0 0.0 0.0 7.185451472694207E-5 7.185451472694207E-5 4 0.0 0.0 0.0 7.185451472694207E-5 7.185451472694207E-5 5 0.0 0.0 0.0 7.185451472694207E-5 7.185451472694207E-5 6 0.0 0.0 0.0 7.185451472694207E-5 7.185451472694207E-5 7 0.0 0.0 0.0 7.185451472694207E-5 7.185451472694207E-5 8 0.0 0.0 0.0 7.185451472694207E-5 7.185451472694207E-5 9 0.0 0.0 0.0 7.185451472694207E-5 7.185451472694207E-5 10-11 0.0 0.0 0.0 7.185451472694207E-5 7.185451472694207E-5 12-13 0.0 0.0 0.0 7.185451472694207E-5 7.185451472694207E-5 14-15 0.0 0.0 0.0 7.185451472694207E-5 7.185451472694207E-5 16-17 0.0 0.0 0.0 1.077817720904131E-4 7.185451472694207E-5 18-19 0.0 0.0 0.0 1.4370902945388413E-4 7.185451472694207E-5 20-21 0.0 0.0 0.0 1.4370902945388413E-4 1.4370902945388413E-4 22-23 0.0 0.0 0.0 1.7963628681735518E-4 2.155635441808262E-4 24-25 0.0 0.0 0.0 4.311270883616524E-4 2.155635441808262E-4 26-27 0.0 0.0 0.0 0.001077817720904131 2.8741805890776826E-4 28-29 0.0 0.0 0.0 0.0019041446402639647 3.233453162712393E-4 30-31 0.0 0.0 0.0 0.0029460351038046247 3.592725736347103E-4 32-33 0.0 0.0 0.0 0.004670543457251234 3.592725736347103E-4 34-35 0.0 0.0 0.0 0.006466906325424786 3.592725736347103E-4 36-37 0.0 0.0 0.0 0.008011778392054041 3.592725736347103E-4 38-39 0.0 0.0 0.0 0.010167413833862302 3.592725736347103E-4 40-41 0.0 0.0 0.0 0.013364939739211225 3.592725736347103E-4 42-43 0.0 0.0 0.0 0.019041446402639647 3.592725736347103E-4 44-45 0.0 0.0 0.0 0.02910107846441154 3.592725736347103E-4 46-47 0.0 0.0 0.0 0.039340346813000776 3.592725736347103E-4 48-49 0.0 0.0 0.0 0.05935182916445414 3.592725736347103E-4 50-51 0.0 0.0 0.0 0.07939923877327099 3.592725736347103E-4 52-53 0.0 0.0 0.0 0.09865624872009146 3.592725736347103E-4 54-55 0.0 0.0 0.0 0.13214045258284646 3.592725736347103E-4 56-57 0.0 0.0 0.0 0.16824734623313486 3.592725736347103E-4 58-59 0.0 0.0 0.0 0.21179118215766174 3.592725736347103E-4 60-61 0.0 0.0 0.0 0.262987523900608 3.592725736347103E-4 62-63 0.0 0.0 0.0 0.33333309381828424 3.592725736347103E-4 64-65 0.0 0.0 0.0 0.3885532883859392 3.592725736347103E-4 66-67 0.0 0.0 0.0 0.47513797863190443 3.592725736347103E-4 68-69 3.592725736347103E-5 0.0 0.0 0.5816263694572326 3.592725736347103E-4 70-71 7.185451472694207E-5 0.0 0.0 0.6518641576028184 3.592725736347103E-4 72-73 7.185451472694207E-5 3.592725736347103E-5 0.0 0.7564124765305191 3.592725736347103E-4 74-75 7.185451472694207E-5 7.185451472694207E-5 0.0 0.8750442803447005 3.592725736347103E-4 76-77 7.185451472694207E-5 7.185451472694207E-5 0.0 0.9737364563221553 3.592725736347103E-4 78-79 7.185451472694207E-5 7.185451472694207E-5 0.0 1.0729675411600623 3.592725736347103E-4 80-81 7.185451472694207E-5 7.185451472694207E-5 0.0 1.216029879981404 3.592725736347103E-4 82-83 7.185451472694207E-5 7.185451472694207E-5 0.0 1.3874747521198878 3.592725736347103E-4 84-85 7.185451472694207E-5 7.185451472694207E-5 0.0 1.6099722569718637 4.311270883616524E-4 86-87 7.185451472694207E-5 7.185451472694207E-5 0.0 1.9079170022871292 4.311270883616524E-4 88-89 7.185451472694207E-5 7.185451472694207E-5 0.0 2.2599681971917818 4.311270883616524E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCGTA 70 0.008395266 27.140802 9 TAAGGTG 895 0.0 25.47568 5 GTAAGGT 940 0.0 23.750763 4 GAGTACT 4690 0.0 21.824432 12-13 AAGGTAA 990 0.0 21.59781 1 TATTCCG 155 3.9062135E-5 21.452303 5 GTACTTT 4810 0.0 21.182352 14-15 AGGTAAG 1005 0.0 20.328419 2 GGGTATA 260 1.48520485E-8 20.10258 1 GTACATG 4945 0.0 19.986164 1 GGTAAGG 1070 0.0 19.977997 3 TACCGTG 145 6.145601E-4 19.655804 5 AGAGTAC 7395 0.0 19.332546 10-11 TAAGGTA 445 0.0 19.214102 4 ACATGGG 4970 0.0 18.829248 3 GTATAGG 530 0.0 18.826773 1 TACATGG 5050 0.0 18.628376 2 ACTTTTT 5585 0.0 18.370565 16-17 CCTACAC 545 0.0 18.303999 3 ACCGTCG 130 0.0075640734 18.269161 8 >>END_MODULE