FastQCFastQC Report
Thu 26 May 2016
SRR936702_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936702_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489520
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37630.2526317202857296No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT36680.24625382673612975No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34940.23457221118212576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30970.20791932971695581No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25870.1736801117138407No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22650.15206240936677587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16400.1101025833825662No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15430.10359041838981685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG46250.024.251081
TACATGG45500.024.2330152
ACATGGG45950.023.0648253
GAGTACT45350.022.10609212-13
GTATAGC5700.021.6785031
GTACTTT48150.021.06444714-15
GTATCAA101600.019.9739991
AGAGTAC68650.019.62223810-11
AGTACTT47000.019.4598612-13
TAGACTG6650.019.29695
TAGCCCT10300.018.9187344
CATGGGG30650.018.297724
TCAACGC109600.018.2131144
ATCAACG111100.018.1377183
CAACGCA110400.018.0380825
AACGCAG112700.017.836256
TATCAAC113450.017.8039052
CTAACAC5650.017.6645153
GTGTAGG7300.017.5780931
ACTTTTT59350.017.56991216-17
Produced by FastQC (version 0.11.5)