FastQCFastQC Report
Thu 26 May 2016
SRR936702_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936702_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1489520
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT67570.45363607068048767No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34770.2334309039153553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31570.2119474730114399No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20580.13816531500080564No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20250.13594983618883935No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC18020.12097857027767334No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17140.11507062677909663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16750.11245233363768192No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA16700.11211665502980825No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC15130.10157634674257479No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGG10400.027.400423
AGGTAAG9850.027.0071372
TAAGGTG10950.026.8870985
GTAAGGT10250.026.4104424
AAGGTAA10300.024.7099821
GAGTACT47650.022.57132712-13
GTACTTT48050.022.23519514-15
AGTACTT48450.021.41457612-13
GTACATG48700.020.8059371
GTGTAGG7250.020.533241
AGAGTAC75900.019.92596810-11
TACATGG49500.019.7691862
ACTTTTT56600.019.00218816-17
TAGCCCT8000.018.9969844
TACTTTT53450.018.83388714-15
GGTGTGC17400.018.8306888
AAGGTGT17200.018.4974866
ACATGGG50600.017.9275993
GTATAGC4153.274181E-1117.3570921
TACACCG2202.9254736E-517.2676685
Produced by FastQC (version 0.11.5)