##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936702_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1489520 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.578041248187336 31.0 31.0 34.0 30.0 34.0 2 31.817001450131585 31.0 31.0 34.0 30.0 34.0 3 32.09345091036038 33.0 31.0 34.0 30.0 34.0 4 35.87310878672324 37.0 35.0 37.0 35.0 37.0 5 35.6884848810355 37.0 35.0 37.0 33.0 37.0 6 35.79250429668618 37.0 35.0 37.0 35.0 37.0 7 35.73742615070627 37.0 35.0 37.0 33.0 37.0 8 35.70695660346957 37.0 35.0 37.0 33.0 37.0 9 37.365210269079974 39.0 37.0 39.0 34.0 39.0 10-11 37.250503853590416 39.0 37.0 39.0 33.5 39.0 12-13 37.13762151565605 39.0 37.0 39.0 33.0 39.0 14-15 38.3036323782158 40.0 38.0 41.0 33.0 41.0 16-17 38.21741769160535 40.0 38.0 41.0 33.0 41.0 18-19 38.24771604275203 40.0 37.5 41.0 33.5 41.0 20-21 38.30400867393523 40.0 38.0 41.0 34.0 41.0 22-23 38.22474186315054 40.0 38.0 41.0 33.5 41.0 24-25 38.113104557172775 40.0 37.0 41.0 33.0 41.0 26-27 37.91636869595575 40.0 37.0 41.0 33.0 41.0 28-29 37.8524084940115 40.0 37.0 41.0 33.0 41.0 30-31 37.87878343358935 40.0 37.0 41.0 33.0 41.0 32-33 37.737949473655945 40.0 37.0 41.0 33.0 41.0 34-35 37.59030493044739 40.0 37.0 41.0 32.5 41.0 36-37 37.37681467855417 40.0 36.5 41.0 31.5 41.0 38-39 37.218886621193406 39.0 36.0 41.0 31.0 41.0 40-41 37.00230544067888 39.0 36.0 41.0 31.0 41.0 42-43 36.787011251946936 39.0 35.5 41.0 31.0 41.0 44-45 36.55125678070788 39.0 35.0 40.5 30.0 41.0 46-47 36.224330992534505 39.0 35.0 40.0 30.0 41.0 48-49 35.80665751382996 39.0 35.0 40.0 28.5 41.0 50-51 35.616344527095976 38.0 35.0 40.0 28.0 41.0 52-53 35.355134875664646 38.0 34.0 40.0 27.5 41.0 54-55 35.083808878027824 38.0 34.0 40.0 27.0 41.0 56-57 34.78141817498255 38.0 34.0 40.0 26.0 41.0 58-59 34.39932696439121 37.5 33.5 40.0 26.0 41.0 60-61 34.00159648745905 37.0 33.0 40.0 25.0 41.0 62-63 33.70243803372899 36.0 33.0 39.0 25.0 40.5 64-65 33.263654734411084 36.0 32.0 39.0 23.5 40.0 66-67 32.84098333691391 35.0 32.0 38.5 23.0 40.0 68-69 32.372619367312964 35.0 31.0 38.0 22.0 40.0 70-71 31.887503356786077 35.0 31.0 37.0 21.0 39.5 72-73 31.462104906278533 34.0 31.0 37.0 20.0 39.0 74-75 31.090183750469947 34.0 30.0 36.0 19.5 39.0 76-77 29.69633573231645 32.5 28.5 35.0 18.0 37.0 78-79 30.50248267898383 34.0 30.0 35.0 18.0 37.0 80-81 30.351864694666737 34.0 30.0 35.0 18.0 37.0 82-83 30.01473998335034 34.0 30.0 35.0 15.0 36.5 84-85 29.581730691766474 34.0 29.0 35.0 9.5 36.0 86-87 29.216247515978303 34.0 29.0 35.0 7.0 36.0 88-89 28.80565014232773 33.5 29.0 35.0 2.0 35.0 90-91 28.353060046189377 33.0 28.5 35.0 2.0 35.0 92-93 27.895899685804824 33.0 27.0 35.0 2.0 35.0 94-95 27.366135399323273 33.0 26.5 34.5 2.0 35.0 96-97 26.677595802674688 32.5 25.0 34.0 2.0 35.0 98-99 25.817003464203232 32.0 24.5 34.0 2.0 35.0 100-101 22.962237499328644 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.39815857051943837 1101 2 0.3314178071275826 1101 3 0.1671064236722941 1101 4 0.15756757219537576 1101 5 0.20079496522006224 1101 6 0.19847858441355726 1101 7 0.10890587117051354 1101 8 0.041831608735527936 1101 9 0.23020977840702983 1101 10-11 0.21734585906209958 1101 12-13 0.22197790606647771 1101 14-15 0.3206144407559677 1101 16-17 0.27160879012259187 1101 18-19 0.3517304518863682 1101 20-21 0.21317062552331834 1101 22-23 0.08908667279860794 1101 24-25 0.005865711185855105 1101 26-27 0.2019398521872091 1101 28-29 0.14935381712069074 1101 30-31 0.05480791332575308 1101 32-33 0.1482001877459851 1101 34-35 0.21946774118508472 1101 36-37 0.19313289788474464 1101 38-39 0.1984297070464649 1101 40-41 0.052312027924969584 1101 42-43 0.13857850458200005 1101 44-45 0.1714274363666135 1101 46-47 0.2460623686178991 1101 48-49 0.19750959488992947 1101 50-51 0.25263084866632113 1101 52-53 0.1466777805851649 1101 54-55 0.17596383994653308 1101 56-57 0.26209769090510804 1101 58-59 0.17174524673971092 1101 60-61 0.3373422010538363 1101 62-63 0.008811058634258018 1101 64-65 0.03438888018717279 1101 66-67 -0.0988663435713093 1101 68-69 -0.14486827388091683 1101 70-71 -0.27860251600637653 1101 72-73 -0.21608781433308266 1101 74-75 -0.10269906184559474 1101 76-77 0.23273907271282823 1101 78-79 0.22270503271988318 1101 80-81 0.15780866566905516 1101 82-83 0.27716209752167487 1101 84-85 0.20135277691819553 1101 86-87 0.16191356977754978 1101 88-89 0.05568584427787826 1101 90-91 -0.062097694653228785 1101 92-93 -0.09869298737644527 1101 94-95 -0.10149927683692894 1101 96-97 -0.12538524821115615 1101 98-99 -0.3242559118419166 1101 100-101 -0.5038066077437655 1102 1 0.3338196611734787 1102 2 0.416879353682031 1102 3 0.1761197850610401 1102 4 0.04483476490096905 1102 5 0.03739128294757421 1102 6 0.04256597701394327 1102 7 -0.00442283302026425 1102 8 -0.11125161062270905 1102 9 0.027755771743869673 1102 10-11 0.06564801582170077 1102 12-13 -0.04096875358634122 1102 14-15 0.10528019562182322 1102 16-17 0.24748268105718552 1102 18-19 0.09178095162334898 1102 20-21 0.1263774289601045 1102 22-23 -0.09183158075215658 1102 24-25 0.038913019620018474 1102 26-27 -0.08049718095324465 1102 28-29 -0.04076955654229408 1102 30-31 0.04217722772168031 1102 32-33 0.12008333696569196 1102 34-35 0.2766842237381226 1102 36-37 0.18742390261909492 1102 38-39 -0.06759126437209062 1102 40-41 -0.09019261875221218 1102 42-43 -0.15239607020320278 1102 44-45 -0.18755709808893783 1102 46-47 -0.17813864839068572 1102 48-49 -0.08376440125245921 1102 50-51 -0.08779671577345738 1102 52-53 -0.2557205360902657 1102 54-55 -0.003446856178314306 1102 56-57 -0.05775179182327861 1102 58-59 -0.08914004170495815 1102 60-61 -0.1153878006996294 1102 62-63 -0.23461675129034631 1102 64-65 -0.2209492927042831 1102 66-67 -0.13439122521255342 1102 68-69 -0.1913050264672762 1102 70-71 -0.13019178481226845 1102 72-73 -0.11324944856068342 1102 74-75 -0.6360336576723498 1102 76-77 -0.39751237389421945 1102 78-79 -0.10201360659275593 1102 80-81 0.11082342982826887 1102 82-83 0.11783697039560082 1102 84-85 0.21716536678317766 1102 86-87 0.08163091152750468 1102 88-89 -0.11091503245366496 1102 90-91 0.03495143738254214 1102 92-93 0.18463270085781858 1102 94-95 -0.06867837555690315 1102 96-97 -0.11510434342419984 1102 98-99 -0.431736045105108 1102 100-101 -0.5284044685187936 1103 1 0.4525756099230591 1103 2 0.45745997965154217 1103 3 0.36429662601521073 1103 4 0.08033903119431329 1103 5 0.04764736138619696 1103 6 0.1580474875019462 1103 7 0.1155759457179002 1103 8 0.2456688824309481 1103 9 0.20746473048370717 1103 10-11 0.2360823872836093 1103 12-13 0.29574531820704664 1103 14-15 0.2890536313842915 1103 16-17 0.18544734177329047 1103 18-19 0.4240456808533537 1103 20-21 0.22864368196633933 1103 22-23 0.21589348856005586 1103 24-25 0.2102047953179138 1103 26-27 0.1847522057548332 1103 28-29 0.21728054768511385 1103 30-31 0.35954018169635305 1103 32-33 0.15800295664907082 1103 34-35 0.2807912768613292 1103 36-37 0.40607304697953595 1103 38-39 0.4012105377173967 1103 40-41 0.40262938681740934 1103 42-43 0.24016103919328202 1103 44-45 0.46691795819835136 1103 46-47 0.5736670544538924 1103 48-49 0.5204778590006853 1103 50-51 0.25848899563116845 1103 52-53 0.36502836631466806 1103 54-55 0.49481410618721355 1103 56-57 0.34699566747592314 1103 58-59 0.37482895280999884 1103 60-61 0.23753432032965804 1103 62-63 0.22284258153094783 1103 64-65 0.2115168887068748 1103 66-67 0.11139009945318179 1103 68-69 0.18814088479852842 1103 70-71 0.2697076011395261 1103 72-73 0.19062528470844953 1103 74-75 0.3318444951299142 1103 76-77 0.3580191579098475 1103 78-79 0.49554294539294474 1103 80-81 0.29144189250611063 1103 82-83 0.5283062934748166 1103 84-85 0.5294673669075429 1103 86-87 0.5508635165294109 1103 88-89 0.4356087409764946 1103 90-91 0.24631082504858526 1103 92-93 0.41469700197393067 1103 94-95 0.5005974217786182 1103 96-97 0.18602689236392678 1103 98-99 0.05754430115063158 1103 100-101 0.1725823166438829 1104 1 0.2944080328850305 1104 2 0.37736619422435425 1104 3 0.05548491829595292 1104 4 -0.033198019202473006 1104 5 -0.04164804553262513 1104 6 0.004203315596349455 1104 7 -0.00971348366820024 1104 8 -0.13291677836124904 1104 9 -0.08453430761447578 1104 10-11 -0.1253616678196252 1104 12-13 -0.08456833049266521 1104 14-15 0.15171981710005156 1104 16-17 -6.729303075161397E-4 1104 18-19 0.0425691615637831 1104 20-21 -0.11870679383152094 1104 22-23 -0.08119074655623137 1104 24-25 -0.07759880494317173 1104 26-27 0.04623662638076098 1104 28-29 -0.11883543019114029 1104 30-31 0.012906838056927938 1104 32-33 0.0030130909717911436 1104 34-35 -0.017805377094489927 1104 36-37 0.10253719896001456 1104 38-39 -0.02636169080299311 1104 40-41 -0.006817004333093735 1104 42-43 -0.029625796493270684 1104 44-45 -0.06172095073016237 1104 46-47 0.10320860517704489 1104 48-49 0.04257841209422253 1104 50-51 0.04886310673082761 1104 52-53 -0.1655867355438616 1104 54-55 0.04316599048416947 1104 56-57 0.2103815618728504 1104 58-59 0.008328042438641603 1104 60-61 -0.14346640109670972 1104 62-63 -0.3066491564195104 1104 64-65 -0.12783262518916416 1104 66-67 -0.0761824726035698 1104 68-69 -0.28059730613898637 1104 70-71 -0.39829713966229363 1104 72-73 -0.2483652336879203 1104 74-75 -0.16069045969505424 1104 76-77 0.16291111572357764 1104 78-79 -0.018396042548932456 1104 80-81 -0.05714617304062486 1104 82-83 -0.04528091323101435 1104 84-85 0.08065600272464835 1104 86-87 -0.09278535495363371 1104 88-89 -0.02333351056082833 1104 90-91 -0.1343858666962383 1104 92-93 -0.12005427769902255 1104 94-95 -0.2150412123208021 1104 96-97 -0.20477234498534713 1104 98-99 -0.09689247098170028 1104 100-101 -0.1362216615072036 1105 1 0.33554224324004167 1105 2 0.3637022320037495 1105 3 0.0818476454664605 1105 4 0.09960418932984538 1105 5 -0.0801719032444339 1105 6 -0.02626608628906979 1105 7 0.05872586750145814 1105 8 -0.12305028449507205 1105 9 -0.05698860721028609 1105 10-11 -0.11981385475188233 1105 12-13 -0.07705742689995532 1105 14-15 0.0458446003597075 1105 16-17 -0.016586109895747825 1105 18-19 -0.0389068623678952 1105 20-21 0.017997776504792284 1105 22-23 -0.023150641455657706 1105 24-25 -0.19729525010564686 1105 26-27 -0.05624264643689969 1105 28-29 -0.11607476479088774 1105 30-31 -0.12326172527267687 1105 32-33 0.01808519967040212 1105 34-35 -0.12718512984775288 1105 36-37 -0.12695383849389685 1105 38-39 -0.13824231643545914 1105 40-41 -0.06821044501577234 1105 42-43 0.014605655563471487 1105 44-45 -0.059999751305369387 1105 46-47 -0.018203417476392758 1105 48-49 -0.001260392268392252 1105 50-51 -0.13960778644651128 1105 52-53 -0.18337527390024633 1105 54-55 -0.14323381186047612 1105 56-57 -0.2168836336225013 1105 58-59 -0.2929749146985543 1105 60-61 -0.2310904535049687 1105 62-63 -0.3020804475114076 1105 64-65 -0.2903205676205616 1105 66-67 -0.3881660683924437 1105 68-69 -0.45016814546411155 1105 70-71 -0.5420644862870603 1105 72-73 -0.5736034261474252 1105 74-75 -0.4515749378316478 1105 76-77 -0.6323330241501317 1105 78-79 -0.4394848040073178 1105 80-81 -0.40068805052981205 1105 82-83 -0.40090255116664153 1105 84-85 -0.43954863566665026 1105 86-87 -0.6383953643629745 1105 88-89 -0.6171671368265059 1105 90-91 -0.6564087568436499 1105 92-93 -0.5175136074461584 1105 94-95 -0.40543998496388056 1105 96-97 -0.39409851183250666 1105 98-99 -0.45986523123285394 1105 100-101 -0.2313771819503323 1106 1 0.32732489743525406 1106 2 0.3130930053155758 1106 3 0.046829186979202575 1106 4 0.03768021847699288 1106 5 -0.12147366445150709 1106 6 0.008468467388262013 1106 7 0.004078087101049732 1106 8 0.019012129686828416 1106 9 -0.03574779048689436 1106 10-11 -0.07897441273858163 1106 12-13 -0.03931631816908521 1106 14-15 0.017020782771815846 1106 16-17 0.055380175551057675 1106 18-19 0.019044155170625743 1106 20-21 0.01831113137458118 1106 22-23 -0.014463497076818044 1106 24-25 -0.012465583642374156 1106 26-27 -0.09018064611403531 1106 28-29 -0.069735952603736 1106 30-31 -0.06633304552664754 1106 32-33 0.09730430250627364 1106 34-35 -0.21948099418676037 1106 36-37 -0.09929337618093115 1106 38-39 0.10657587541188462 1106 40-41 0.023024749706777925 1106 42-43 -0.18949775060863772 1106 44-45 -0.2698713178802734 1106 46-47 -0.13631483032509095 1106 48-49 -0.21797730582732555 1106 50-51 -0.2780740210958541 1106 52-53 -0.07289594534915267 1106 54-55 -0.2170688516351973 1106 56-57 -0.2819350761768078 1106 58-59 -0.4222790643210672 1106 60-61 -0.1917493806751267 1106 62-63 -0.4003397152426871 1106 64-65 -0.28638454374638656 1106 66-67 -0.25051119068343297 1106 68-69 -0.18868495942263053 1106 70-71 -0.11951939029856362 1106 72-73 -0.064801442117151 1106 74-75 -0.07220362960701365 1106 76-77 -0.12854518549781702 1106 78-79 -0.13421576758211629 1106 80-81 -0.19420885037473923 1106 82-83 -0.16568788360327957 1106 84-85 0.12552929635949184 1106 86-87 -0.1060803902073495 1106 88-89 -0.18383412552204348 1106 90-91 -0.26714503327157146 1106 92-93 0.054202897863266486 1106 94-95 0.17098120636427794 1106 96-97 0.10202358531093125 1106 98-99 -0.1734947189389331 1106 100-101 -0.020731032054822407 1107 1 0.4855632863298105 1107 2 0.5599533246257202 1107 3 0.24158088383610732 1107 4 0.2292296223318857 1107 5 -0.08658195212915132 1107 6 0.15195269709660408 1107 7 0.19204193470984876 1107 8 0.09246332876531227 1107 9 0.09108999682351282 1107 10-11 0.23134675258406645 1107 12-13 0.2540636841751862 1107 14-15 0.317705133235485 1107 16-17 0.1506255188393979 1107 18-19 0.028236781000295252 1107 20-21 0.16413394148261062 1107 22-23 0.2666288917114841 1107 24-25 0.1641279102426978 1107 26-27 0.11100135628252161 1107 28-29 0.19568670369303476 1107 30-31 0.18218681627933364 1107 32-33 0.15368363276955677 1107 34-35 0.0835533207333512 1107 36-37 0.19086156504781826 1107 38-39 0.3021733655225134 1107 40-41 0.40446591226351813 1107 42-43 0.38523921443555764 1107 44-45 0.11064351976199305 1107 46-47 0.48594045250968065 1107 48-49 0.5209643479303807 1107 50-51 0.49205720756430793 1107 52-53 0.2423102452165864 1107 54-55 0.45406619785172353 1107 56-57 0.46233874327542424 1107 58-59 0.1280325636918107 1107 60-61 0.3392420273134533 1107 62-63 0.31694250564343207 1107 64-65 0.42185852526039724 1107 66-67 0.4534118396006974 1107 68-69 0.5501879683628133 1107 70-71 0.40272168354686144 1107 72-73 0.624088308774386 1107 74-75 0.6810612757071475 1107 76-77 0.4885514331000209 1107 78-79 0.5114200488529228 1107 80-81 0.4876864517201831 1107 82-83 0.3419023308301874 1107 84-85 0.6656182522222416 1107 86-87 0.8108746022918183 1107 88-89 0.886426375427046 1107 90-91 0.7513253879975572 1107 92-93 1.1011370448896258 1107 94-95 1.1231462431432142 1107 96-97 0.9038716393539978 1107 98-99 0.8297362450208325 1107 100-101 0.87793357592464 1108 1 0.2505144719461576 1108 2 0.24549095887855898 1108 3 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-1.1189446615377605 2316 52-53 -1.0394609949250366 2316 54-55 -0.6362865001895202 2316 56-57 -0.42986421385679563 2316 58-59 -0.847378562784094 2316 60-61 -0.764295894184265 2316 62-63 -0.6528977849031676 2316 64-65 -0.6432056776359545 2316 66-67 -0.703503078265193 2316 68-69 -0.7686179337448635 2316 70-71 -0.4853127200880074 2316 72-73 -0.5955925762378449 2316 74-75 -0.6714936196687198 2316 76-77 -0.7295466415728882 2316 78-79 -0.4693330625182064 2316 80-81 -0.45853963365067685 2316 82-83 -0.3498365419341063 2316 84-85 -0.7839479033539156 2316 86-87 -0.5279367703585045 2316 88-89 -0.45720667272892257 2316 90-91 -0.8161874905715933 2316 92-93 -1.2538385656077367 2316 94-95 -1.31399995710904 2316 96-97 -1.0013852482111538 2316 98-99 -0.9503838029983775 2316 100-101 -2.068285519308386 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 22.0 5 46.0 6 99.0 7 250.0 8 549.0 9 995.0 10 1587.0 11 2532.0 12 3608.0 13 4669.0 14 6454.0 15 8266.0 16 10079.0 17 12399.0 18 11306.0 19 8767.0 20 8354.0 21 8751.0 22 9242.0 23 10516.0 24 12482.0 25 14585.0 26 18175.0 27 22969.0 28 28801.0 29 35379.0 30 43869.0 31 54734.0 32 69157.0 33 88466.0 34 117041.0 35 161250.0 36 231320.0 37 297067.0 38 175569.0 39 10156.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.15037632759636 19.037385764964213 14.840715578849656 22.97152232858977 2 16.05195237698776 20.514595491766954 38.684096030251844 24.749356100993445 3 20.49681059121387 23.43042214599778 29.15302918304004 26.919738079748313 4 13.798632303028471 16.76728001530854 36.15513994219013 33.27894773947286 5 14.39020624093668 37.24031902894892 33.454737096514314 14.914737633600087 6 32.95726139964552 36.01583060314732 16.470540845373005 14.556367151834149 7 29.655593748321607 32.071204146302165 19.76468929588055 18.508512809495674 8 26.94666738278103 34.312933025404156 18.826199043987323 19.914200547827487 9 26.126460612147888 17.320087227180075 19.680095391300377 36.873356769371654 10-11 25.59784520609379 26.09119338093716 26.593437062777436 21.717524350191606 12-13 26.75247966120584 23.29290414751618 26.92881187068569 23.0258043205923 14-15 23.072489532691268 25.35060826703394 25.391158283700406 26.185743916574385 16-17 22.849609270100434 28.180856920350177 26.082899189000486 22.8866346205489 18-19 22.76001664965895 27.151901283634995 28.047088995112517 22.040993071593533 20-21 23.48646660886279 25.885965239125774 28.21171525448307 22.415852897528364 22-23 23.08168202583213 25.84488837372772 28.19483565474758 22.878593945692568 24-25 23.388172028572964 26.046142381438315 28.12056904237607 22.445116547612653 26-27 22.948164509372145 26.086457382243943 28.350408185187177 22.614969923196735 28-29 22.706240936677588 26.995642891669803 27.867064557709863 22.431051613942746 30-31 22.979046941296524 26.135567162575864 27.94242440517751 22.942961490950104 32-33 22.899465173143984 26.309875967972157 27.941984109613323 22.84867474927054 34-35 23.532608640921435 26.200003961046132 27.82732255772134 22.4400648403111 36-37 23.636794538215273 25.846262092755406 28.009236802326186 22.507706566703135 38-39 23.14200864239014 26.415012636633918 27.81029574322172 22.63268297775422 40-41 23.376288555971367 26.261331303064605 27.687454094039577 22.67492604692445 42-43 23.130632944369356 26.50400411994298 27.25309381800407 23.112269117683592 44-45 23.306333040168113 26.300780804167868 27.384003920805107 23.008882234858913 46-47 23.970693481965064 26.705977450120777 26.87022741890221 22.45310164901195 48-49 23.856858312974893 26.789178765628886 26.597786483293547 22.756176438102674 50-51 23.22359724285723 27.139340173600363 27.0567964160098 22.58026616753261 52-53 23.80751448404766 27.00305172900539 26.500696382336564 22.68873740461039 54-55 23.2508000198746 27.0128070323862 26.73530118750747 23.001091760231727 56-57 23.664766871729938 26.998594791800244 26.562094022057675 22.774544314412147 58-59 23.9332194252453 26.700011077505614 26.3436846469129 23.023084850336186 60-61 23.352199674370983 26.947379022105842 27.15631871359753 22.544102589925643 62-63 23.139186115796807 27.036061666540785 27.09017514707487 22.734577070587545 64-65 23.019794583810793 27.42948408244447 26.72525660236345 22.825464731381285 66-67 23.260588535636515 26.931475588906572 26.606260656009006 23.201675219447907 68-69 23.27468458745869 26.925866933419712 26.68985090809608 23.109597571025517 70-71 23.561287827697942 26.990759653991386 26.367866274633677 23.08008624367699 72-73 23.45928450728054 26.812984594355626 26.69432212680738 23.033408771556456 74-75 23.293376389709437 27.67764111928675 26.181555131854555 22.847427359149254 76-77 23.62813265046138 26.911429959244536 26.261041085442805 23.19939630485128 78-79 23.51086023072073 27.248707978672332 26.24020282905166 23.000228961555276 80-81 23.78772465242455 27.233598232661514 26.17168430983485 22.806992805079084 82-83 23.654996332212576 27.339804977783448 26.286839441556282 22.718359248447694 84-85 23.385880643593207 27.739581626815845 26.092092859617505 22.78244486997344 86-87 23.449464777005808 27.6401676590016 25.843255283826817 23.06711228016578 88-89 23.169329584138946 27.65459550539915 25.66403923088237 23.512035679579537 90-91 23.459198139023886 27.399233502405124 25.937085952625427 23.204482405945562 92-93 23.57791860987774 27.422044128808672 26.165968604489954 22.834068656823636 94-95 23.249078639132037 27.37843374359546 26.145364525877667 23.227123091394837 96-97 23.16531194061318 27.398791690894175 26.474196195128897 22.961700173363752 98-99 23.104405657558573 27.635790470243432 25.745416578540087 23.514387293657908 100-101 22.76466462793342 27.625495736822792 25.86284981066907 23.746989824574715 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 120.0 1 87.5 2 65.0 3 83.5 4 85.5 5 95.5 6 163.5 7 319.0 8 478.0 9 574.5 10 616.0 11 649.5 12 690.0 13 774.0 14 901.0 15 1027.0 16 1173.5 17 1308.5 18 1523.0 19 1717.5 20 1900.0 21 2189.0 22 2548.5 23 3099.0 24 3893.5 25 4906.5 26 6212.0 27 7951.5 28 9938.5 29 12408.0 30 15345.5 31 18041.5 32 21413.5 33 24104.0 34 25683.5 35 27597.0 36 30576.0 37 33745.5 38 35718.0 39 37220.0 40 39025.5 41 41982.0 42 44074.5 43 45880.5 44 48743.0 45 51796.5 46 54060.0 47 58674.0 48 67414.0 49 66525.5 50 58309.5 51 54588.0 52 53561.0 53 54295.5 54 54580.0 55 51910.5 56 47065.0 57 42855.0 58 40002.5 59 35791.0 60 29695.0 61 24672.5 62 21033.0 63 16413.5 64 11703.0 65 8793.5 66 6794.0 67 5396.0 68 4113.5 69 3070.5 70 2394.5 71 1728.5 72 1332.0 73 1121.5 74 871.0 75 660.5 76 515.0 77 360.5 78 256.0 79 197.0 80 140.0 81 89.0 82 58.5 83 42.0 84 23.0 85 11.5 86 8.0 87 4.0 88 2.0 89 0.5 90 1.0 91 1.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0610129437671196 2 0.010473172565658736 3 0.003961007572909393 4 0.010406036844084 5 0.0 6 0.0 7 0.0 8 0.0 9 0.005102314839679897 10-11 2.6854288629894193E-4 12-13 1.3427144314947097E-4 14-15 1.0070358236210323E-4 16-17 0.0 18-19 0.0 20-21 3.356786078736774E-5 22-23 0.0012420108491326064 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.004430957623932542 34-35 9.734679628336646E-4 36-37 0.0029204038885009935 38-39 1.0070358236210323E-4 40-41 0.004162414737633601 42-43 0.012017294161877651 44-45 0.0020140716472420643 46-47 0.0014769858746441808 48-49 0.0114466405284924 50-51 4.699500510231484E-4 52-53 0.0024504538374778453 54-55 0.012218701326601857 56-57 0.004095279016058865 58-59 0.0010070358236210321 60-61 0.006377893549599872 62-63 0.0038603039905472903 64-65 0.020107148611633277 66-67 0.0029204038885009935 68-69 1.678393039368387E-4 70-71 0.001309146570707342 72-73 6.713572157473548E-5 74-75 0.0 76-77 0.0017790966217304904 78-79 0.012554379934475536 80-81 0.01846232343305226 82-83 0.013594983618883937 84-85 0.008861915247865084 86-87 0.012587947795262905 88-89 0.03282936785004565 90-91 0.02745851012406681 92-93 0.0026518610022020516 94-95 0.009868951071486116 96-97 0.01114452978140609 98-99 0.012420108491326064 100-101 0.013192169289435524 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1489520.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.60097154477333 #Duplication Level Percentage of deduplicated Percentage of total 1 80.97143957660542 51.49862224451015 2 11.505541787651843 14.63527271687291 3 3.3957843968412775 6.479255603870622 4 1.3956291986056208 3.550534917902836 5 0.7681403731332334 2.4427237007019174 6 0.44789807087049677 1.7092051476236003 7 0.29884926333534784 1.3304972445497552 8 0.2101694204959796 1.069358346603704 9 0.15913712160235668 0.9109147988473759 >10 0.7819283058038035 8.742196898352208 >50 0.04222356974629028 1.8444146021296464 >100 0.0189244160212692 2.3769296315172763 >500 0.002431548380535797 1.0443503360619906 >1k 0.0017972314117003715 1.911392415866225 >5k 1.0571949480590421E-4 0.45433139458993504 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6757 0.45363607068048767 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3477 0.2334309039153553 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3157 0.2119474730114399 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2058 0.13816531500080564 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2025 0.13594983618883935 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1802 0.12097857027767334 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1714 0.11507062677909663 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1675 0.11245233363768192 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1670 0.11211665502980825 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1513 0.10157634674257479 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.356786078736774E-5 0.0 18-19 0.0 0.0 0.0 1.3427144314947097E-4 0.0 20-21 0.0 0.0 0.0 2.0140716472420646E-4 0.0 22-23 0.0 0.0 0.0 2.349750255115742E-4 3.356786078736774E-5 24-25 0.0 0.0 0.0 3.356786078736774E-4 6.713572157473548E-5 26-27 0.0 0.0 0.0 5.035179118105162E-4 6.713572157473548E-5 28-29 0.0 0.0 0.0 9.06332241258929E-4 6.713572157473548E-5 30-31 0.0 0.0 0.0 0.0016783930393683873 1.0070358236210323E-4 32-33 0.0 0.0 0.0 0.0028532681669262584 1.3427144314947097E-4 34-35 0.0 0.0 0.0 0.0043302540415704385 1.3427144314947097E-4 36-37 0.0 0.0 0.0 0.005907943498576722 1.3427144314947097E-4 38-39 0.0 0.0 0.0 0.0077206079810945814 1.3427144314947097E-4 40-41 0.0 0.0 0.0 0.01010392609699769 1.3427144314947097E-4 42-43 0.0 0.0 0.0 0.014836994468016541 1.3427144314947097E-4 44-45 0.0 0.0 0.0 0.02396745260218057 1.3427144314947097E-4 46-47 0.0 0.0 0.0 0.03373570009130458 1.3427144314947097E-4 48-49 0.0 0.0 0.0 0.05172807347333369 1.3427144314947097E-4 50-51 0.0 0.0 0.0 0.06867984317095441 1.3427144314947097E-4 52-53 0.0 0.0 0.0 0.0834497019173962 1.3427144314947097E-4 54-55 0.0 0.0 0.0 0.11127745851012406 2.0140716472420646E-4 56-57 0.0 0.0 0.0 0.14041436167355925 2.0140716472420646E-4 58-59 0.0 0.0 0.0 0.17737257640045115 2.0140716472420646E-4 60-61 0.0 0.0 0.0 0.22369622428701863 2.0140716472420646E-4 62-63 0.0 0.0 0.0 0.28818008485955204 2.0140716472420646E-4 64-65 0.0 0.0 0.0 0.3364171008109995 2.0140716472420646E-4 66-67 0.0 0.0 0.0 0.4121461947473012 2.0140716472420646E-4 68-69 0.0 0.0 0.0 0.5097279660561791 2.0140716472420646E-4 70-71 0.0 0.0 0.0 0.5683038831301359 2.0140716472420646E-4 72-73 0.0 0.0 0.0 0.6566209248617004 2.0140716472420646E-4 74-75 0.0 0.0 0.0 0.7681333583973361 2.0140716472420646E-4 76-77 0.0 0.0 0.0 0.8577931145603953 2.0140716472420646E-4 78-79 0.0 0.0 0.0 0.9411756807562168 2.0140716472420646E-4 80-81 0.0 0.0 0.0 1.0664173693538859 2.349750255115742E-4 82-83 0.0 0.0 0.0 1.2276773725764003 2.6854288629894193E-4 84-85 0.0 0.0 0.0 1.4331798163166658 3.0211074708630966E-4 86-87 0.0 0.0 0.0 1.7167611042483486 3.6924646866104513E-4 88-89 0.0 0.0 0.0 2.0470352865352597 4.028143294484129E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTAAGG 1040 0.0 27.40042 3 AGGTAAG 985 0.0 27.007137 2 TAAGGTG 1095 0.0 26.887098 5 GTAAGGT 1025 0.0 26.410442 4 AAGGTAA 1030 0.0 24.709982 1 GAGTACT 4765 0.0 22.571327 12-13 GTACTTT 4805 0.0 22.235195 14-15 AGTACTT 4845 0.0 21.414576 12-13 GTACATG 4870 0.0 20.805937 1 GTGTAGG 725 0.0 20.53324 1 AGAGTAC 7590 0.0 19.925968 10-11 TACATGG 4950 0.0 19.769186 2 ACTTTTT 5660 0.0 19.002188 16-17 TAGCCCT 800 0.0 18.996984 4 TACTTTT 5345 0.0 18.833887 14-15 GGTGTGC 1740 0.0 18.830688 8 AAGGTGT 1720 0.0 18.497486 6 ACATGGG 5060 0.0 17.927599 3 GTATAGC 415 3.274181E-11 17.357092 1 TACACCG 220 2.9254736E-5 17.267668 5 >>END_MODULE