FastQCFastQC Report
Thu 26 May 2016
SRR936701_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936701_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543273
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40010.2592541954663886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38010.24629472556054566No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35830.23216890336317683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30760.1993166471518649No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25710.1665939856396114No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22620.146571604635084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16820.1089891419081394No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT16060.10406454334391906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG46800.023.8595871
TACATGG46650.023.2233092
GTATAGA4850.022.5334131
ACATGGG48250.021.1729833
GAGTACT46250.021.01289212-13
GTACTTT46700.020.50545914-15
GTATCAA94450.020.2742141
TAACGCA1908.223178E-620.0087324
AGTACTT47100.019.72559712-13
AGAGTAC69300.019.64601710-11
CCGTATG1250.00601912519.0070619
ACCGTAT1250.00601912519.0070618
CGTACAC1507.705097E-419.0064453
GGTATCA74850.018.9175721
GTAAGGT6650.018.5795354
GTATTCG1300.007549489418.2754291
GTCCTAA5500.018.1425171
GTGTAGC9000.017.9505331
TAAGGTG7700.017.896265
TCAACGC106750.017.8063644
Produced by FastQC (version 0.11.5)