##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936701_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1543273 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.421579331719016 31.0 31.0 34.0 30.0 34.0 2 31.483793858895996 33.0 31.0 34.0 28.0 34.0 3 31.529949658939152 33.0 31.0 34.0 28.0 34.0 4 35.139724468710334 37.0 35.0 37.0 32.0 37.0 5 35.1085679591362 37.0 35.0 37.0 32.0 37.0 6 35.22473081561072 37.0 35.0 37.0 32.0 37.0 7 35.08740903262093 37.0 35.0 37.0 32.0 37.0 8 35.03137098880107 37.0 35.0 37.0 32.0 37.0 9 36.54532606998244 39.0 37.0 39.0 32.0 39.0 10-11 36.44912468500388 39.0 37.0 39.0 32.0 39.0 12-13 36.345375380765425 39.0 37.0 39.0 32.0 39.0 14-15 37.43005126118321 40.0 37.0 41.0 32.0 41.0 16-17 37.300097260821644 40.0 37.0 41.0 32.0 41.0 18-19 37.23444944607986 40.0 37.0 41.0 31.0 41.0 20-21 37.13221089204567 39.5 36.5 41.0 31.0 41.0 22-23 36.98882018929898 39.0 36.0 41.0 31.0 41.0 24-25 37.12454471762287 39.0 36.5 41.0 31.0 41.0 26-27 37.097714727076806 39.0 36.5 41.0 31.0 41.0 28-29 36.99986198164551 39.0 36.0 41.0 30.5 41.0 30-31 36.82063056892721 39.0 36.0 41.0 30.0 41.0 32-33 36.64141114371858 39.0 36.0 41.0 30.0 41.0 34-35 36.422741796169575 39.0 35.0 41.0 30.0 41.0 36-37 36.232635444279786 39.0 35.0 40.0 30.0 41.0 38-39 36.02301180672506 39.0 35.0 40.0 29.0 41.0 40-41 35.84158927163243 38.0 35.0 40.0 28.5 41.0 42-43 35.621497298274505 38.0 35.0 40.0 27.0 41.0 44-45 35.35674439972708 38.0 34.5 40.0 26.5 41.0 46-47 35.1081824149065 38.0 34.0 40.0 26.0 41.0 48-49 35.03088468469286 38.0 34.0 40.0 25.5 41.0 50-51 34.12139232656827 37.5 33.0 39.5 24.0 40.5 52-53 34.27541173855825 38.0 33.5 39.5 24.0 40.5 54-55 34.690243722270786 38.0 34.0 40.0 25.0 41.0 56-57 34.57068062487972 38.0 34.0 40.0 24.5 41.0 58-59 34.29535733470358 38.0 34.0 40.0 23.5 41.0 60-61 33.95825106769833 37.5 33.0 40.0 23.0 41.0 62-63 33.62617437096353 37.0 33.0 40.0 22.0 41.0 64-65 33.27156957971791 36.0 32.5 39.0 22.0 41.0 66-67 32.864113478302286 36.0 32.0 39.0 20.5 41.0 68-69 32.434113407025194 35.0 31.5 39.0 19.5 40.0 70-71 31.983334769674585 35.0 31.0 38.0 18.0 40.0 72-73 31.59457464751862 35.0 31.0 37.0 17.5 39.5 74-75 31.167931402933895 35.0 31.0 37.0 14.0 39.0 76-77 30.727783742733784 34.5 30.0 36.0 10.0 39.0 78-79 30.287873888806452 34.0 30.0 36.0 7.5 38.5 80-81 29.824721549589736 34.0 29.5 35.0 6.5 37.0 82-83 29.348714712173415 34.0 29.0 35.0 2.0 37.0 84-85 29.017746698089063 34.0 29.0 35.0 2.0 36.0 86-87 28.718690730674354 34.0 29.0 35.0 2.0 36.0 88-89 28.30835892288662 34.0 28.0 35.0 2.0 35.5 90-91 27.890286423724124 34.0 27.0 35.0 2.0 35.0 92-93 27.51402635826584 33.0 26.5 35.0 2.0 35.0 94-95 27.056060398905444 33.0 25.5 35.0 2.0 35.0 96-97 26.467404665279574 33.0 25.0 35.0 2.0 35.0 98-99 25.748152789558297 33.0 23.5 35.0 2.0 35.0 100-101 23.27464745382055 29.5 9.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4218665658319978 1101 2 0.362671528722295 1101 3 0.4228006689944017 1101 4 0.39634370185882517 1101 5 0.42253846477326107 1101 6 0.27595263914366797 1101 7 0.4327866168863679 1101 8 0.5144377580679773 1101 9 0.6060196580790631 1101 10-11 0.532521312181359 1101 12-13 0.6439431881204101 1101 14-15 0.9649430756964534 1101 16-17 0.883204036004166 1101 18-19 0.8096768838101767 1101 20-21 0.7503853559423703 1101 22-23 0.7272759342477499 1101 24-25 0.5917732871430275 1101 26-27 0.5945115200307072 1101 28-29 0.5605073669328817 1101 30-31 0.5384863972759462 1101 32-33 0.5157164953255275 1101 34-35 0.5883468524648165 1101 36-37 0.48545933700429345 1101 38-39 0.4221990855578355 1101 40-41 0.422282147714661 1101 42-43 0.3598463742388063 1101 44-45 0.5748829942906113 1101 46-47 0.5409033843043574 1101 48-49 0.6825307959749267 1101 50-51 0.6683105549271389 1101 52-53 0.7495736742801782 1101 54-55 0.8672200363924034 1101 56-57 0.6849787876102837 1101 58-59 0.6276504841160744 1101 60-61 0.6183439396070654 1101 62-63 0.569411818125424 1101 64-65 0.664175291631814 1101 66-67 0.7673561873568602 1101 68-69 0.7381542198946462 1101 70-71 0.7455470736642802 1101 72-73 0.7958847338045594 1101 74-75 0.7174619638452135 1101 76-77 0.6237564030636733 1101 78-79 0.6019411244169355 1101 80-81 0.7168694227609897 1101 82-83 0.803026489750053 1101 84-85 0.6756483067306647 1101 86-87 0.4546475321091954 1101 88-89 0.637186943914454 1101 90-91 0.4286711426397858 1101 92-93 0.3279290430950752 1101 94-95 0.08304587255832274 1101 96-97 0.0742366464544233 1101 98-99 0.18774433105584976 1101 100-101 0.5389139895525901 1102 1 0.2561054019313289 1102 2 0.3103522040071347 1102 3 0.235348663245599 1102 4 0.2693578513050383 1102 5 0.2712468434850166 1102 6 0.21427857810656548 1102 7 0.31520723540529616 1102 8 0.2595146593408728 1102 9 0.40746343293166376 1102 10-11 0.4175257391037306 1102 12-13 0.3547486239391944 1102 14-15 0.2564239849642149 1102 16-17 0.2877742203721496 1102 18-19 0.374173613060897 1102 20-21 0.4574904181537036 1102 22-23 0.3206163664641082 1102 24-25 0.16908411098169296 1102 26-27 0.1526891746079002 1102 28-29 0.21611287991968453 1102 30-31 -0.010015616259707372 1102 32-33 0.040623928729040415 1102 34-35 -0.2356968595540465 1102 36-37 0.005189992592967485 1102 38-39 -0.08479467040692157 1102 40-41 0.0316310371580002 1102 42-43 0.16471919519876366 1102 44-45 -0.29248029517945184 1102 46-47 -0.07268085466646568 1102 48-49 0.18163409496167304 1102 50-51 0.19313688646617777 1102 52-53 0.07674209833353274 1102 54-55 0.2861860711471138 1102 56-57 0.3326522149211968 1102 58-59 0.30636032858910767 1102 60-61 0.18421791862620296 1102 62-63 0.2688093740762767 1102 64-65 0.39561657145696216 1102 66-67 0.493591724415559 1102 68-69 0.1601863631771181 1102 70-71 0.09216000652864054 1102 72-73 0.017381604781459004 1102 74-75 -0.07844546363059024 1102 76-77 0.32327911454958524 1102 78-79 0.10072756030524133 1102 80-81 0.2941025127528043 1102 82-83 0.252852457543181 1102 84-85 0.27881674910603493 1102 86-87 0.3525656122205909 1102 88-89 0.6295947585775643 1102 90-91 0.5626898181628128 1102 92-93 0.5116568296716117 1102 94-95 0.7282056809531454 1102 96-97 0.28641394321341807 1102 98-99 0.47785189745564693 1102 100-101 0.2061287898614772 1103 1 0.2837700537030159 1103 2 0.3799228332884681 1103 3 0.35865570671963454 1103 4 0.4944605651104652 1103 5 0.39832929119769744 1103 6 0.33913158099494467 1103 7 0.35944033749045445 1103 8 0.37710934730863244 1103 9 0.6018567789215297 1103 10-11 0.5459480092138875 1103 12-13 0.5347014648101478 1103 14-15 0.3372126425443014 1103 16-17 0.55578917683372 1103 18-19 0.5104416408412433 1103 20-21 0.45053305509225794 1103 22-23 0.5907422150910477 1103 24-25 0.5647628188150051 1103 26-27 0.5592589018522034 1103 28-29 0.17382068540492668 1103 30-31 0.2358448632223542 1103 32-33 0.597333981939947 1103 34-35 0.38222238166527944 1103 36-37 0.5161409022424763 1103 38-39 0.37390957767999566 1103 40-41 0.5344666148518726 1103 42-43 0.6591657739245491 1103 44-45 0.5453831867271433 1103 46-47 0.7543999286705656 1103 48-49 0.8215108165672831 1103 50-51 0.8021326185704538 1103 52-53 0.7038943952537835 1103 54-55 0.6567024205001104 1103 56-57 0.6978416072276303 1103 58-59 0.6872378256300635 1103 60-61 0.5436826772453429 1103 62-63 0.6950188704034659 1103 64-65 0.6871284193559504 1103 66-67 0.6994976386243579 1103 68-69 0.6258337862051988 1103 70-71 0.7326428610038214 1103 72-73 0.872951178684918 1103 74-75 0.8644900529997948 1103 76-77 0.9530983688110659 1103 78-79 0.7662802776304041 1103 80-81 0.8251242920631405 1103 82-83 0.7335829438300898 1103 84-85 0.7203012575886234 1103 86-87 0.659242105070053 1103 88-89 0.7468258759629762 1103 90-91 0.8588923404831803 1103 92-93 0.8076925051543746 1103 94-95 0.9411546326420535 1103 96-97 0.973215417031696 1103 98-99 1.2281233816803656 1103 100-101 1.7156554305129532 1104 1 0.2696982713267708 1104 2 0.3214105049785019 1104 3 0.457546764551946 1104 4 0.43944775335977226 1104 5 0.44526932854721224 1104 6 0.2572266645284742 1104 7 0.43119414158610425 1104 8 0.4178486573255711 1104 9 0.7137047339558293 1104 10-11 0.5392038396462056 1104 12-13 0.41361099167824733 1104 14-15 0.4551653224973933 1104 16-17 0.3475296548800273 1104 18-19 0.5942714628481909 1104 20-21 0.6345291098501988 1104 22-23 0.3234889241511638 1104 24-25 0.6169961321097404 1104 26-27 0.16394981979527756 1104 28-29 0.5774396906827732 1104 30-31 0.532808901175315 1104 32-33 0.28243692167101386 1104 34-35 0.21179923959770974 1104 36-37 0.26156250141411164 1104 38-39 0.30635102192202623 1104 40-41 0.3751074652504016 1104 42-43 0.332922142656102 1104 44-45 0.3150025371565235 1104 46-47 0.4865126217897142 1104 48-49 0.2780694386126399 1104 50-51 0.23954504494003004 1104 52-53 0.5346752834589736 1104 54-55 0.7857650940758987 1104 56-57 0.6432050906523514 1104 58-59 0.6276854711708637 1104 60-61 0.627875936961594 1104 62-63 0.7742285762475021 1104 64-65 0.5514238644985667 1104 66-67 0.686575027998721 1104 68-69 0.6276072593668047 1104 70-71 0.7778954273540641 1104 72-73 0.6852607453254542 1104 74-75 0.3554461113235483 1104 76-77 0.4013707825174677 1104 78-79 0.5048565618341243 1104 80-81 0.545581549274182 1104 82-83 0.6503759946267955 1104 84-85 0.7138488096413589 1104 86-87 0.6843338788060755 1104 88-89 0.7687258551624545 1104 90-91 0.9277597862932474 1104 92-93 0.753048845542363 1104 94-95 0.7754910388145504 1104 96-97 1.080730977422931 1104 98-99 1.322480325512096 1104 100-101 1.6623438043920693 1105 1 0.368902621992806 1105 2 0.3130047052834932 1105 3 0.3398575797330068 1105 4 0.35111207869080374 1105 5 0.5301418929935906 1105 6 0.41697520635997876 1105 7 0.3409449203624817 1105 8 0.45928901973620384 1105 9 0.7063742656162688 1105 10-11 0.6158514773685084 1105 12-13 0.43261000358774737 1105 14-15 0.33604449296153405 1105 16-17 0.5151276362609778 1105 18-19 0.5588933808175014 1105 20-21 0.5727207252695266 1105 22-23 0.47889092231814345 1105 24-25 0.6034527091750519 1105 26-27 0.5550145991622131 1105 28-29 0.3678991527678406 1105 30-31 0.25942611644738633 1105 32-33 0.46951357985432907 1105 34-35 0.44999104675516577 1105 36-37 0.4418545058061838 1105 38-39 0.5168505700591624 1105 40-41 0.40706845421011195 1105 42-43 0.554168453294885 1105 44-45 0.32058882762445506 1105 46-47 0.44648920417255056 1105 48-49 0.5592474968677905 1105 50-51 0.6155756373980736 1105 52-53 0.63021264235568 1105 54-55 0.7481361661604922 1105 56-57 0.9283879193522324 1105 58-59 0.7465557628812576 1105 60-61 0.48895894423358044 1105 62-63 0.7418237135128223 1105 64-65 0.821023975451034 1105 66-67 0.7694065400328469 1105 68-69 0.6114001144370675 1105 70-71 0.7274331467184645 1105 72-73 0.5709221843584835 1105 74-75 0.7409923476957623 1105 76-77 0.41871285740526787 1105 78-79 0.6058213881129859 1105 80-81 0.5248084803008624 1105 82-83 0.7020550134899928 1105 84-85 0.7304765002066915 1105 86-87 0.8818748053102929 1105 88-89 0.8678956929647761 1105 90-91 0.9783166577404998 1105 92-93 0.8229444249558213 1105 94-95 0.8679075400538139 1105 96-97 0.9653038232130626 1105 98-99 1.0923154754577062 1105 100-101 1.4971965558981708 1106 1 -0.11910019942676442 1106 2 2.2419728793465765E-5 1106 3 0.04421726019972638 1106 4 0.11226341179789756 1106 5 0.2511883719889738 1106 6 0.106270131423706 1106 7 0.08116913172040086 1106 8 0.2744013678168997 1106 9 0.460581642686158 1106 10-11 0.3384028955551912 1106 12-13 0.2918575613049583 1106 14-15 0.31993672729777956 1106 16-17 0.5595523623299457 1106 18-19 0.4418730921341165 1106 20-21 0.2241335518034333 1106 22-23 0.32080539069701075 1106 24-25 0.25249870473260927 1106 26-27 0.08219363707998895 1106 28-29 0.3270548026741835 1106 30-31 0.2058514078927871 1106 32-33 0.012345453428515896 1106 34-35 -0.01994670038364177 1106 36-37 0.03499071304532464 1106 38-39 0.09765091911765467 1106 40-41 -0.08106085994321433 1106 42-43 -0.10594130347717368 1106 44-45 0.1450315938447133 1106 46-47 0.11685989445756917 1106 48-49 0.12593750465878628 1106 50-51 -0.019771758422102437 1106 52-53 0.04638090602868061 1106 54-55 -0.04182392973500271 1106 56-57 -0.07132017433910676 1106 58-59 -0.018473315084008846 1106 60-61 0.09515480073449112 1106 62-63 0.11945112634892752 1106 64-65 -0.061134984092461764 1106 66-67 0.28411161906605287 1106 68-69 0.2036790389381551 1106 70-71 0.34653996803971054 1106 72-73 0.3040764590617755 1106 74-75 0.264092549742724 1106 76-77 0.42802074242627697 1106 78-79 0.4155631722306872 1106 80-81 0.4303127749084119 1106 82-83 0.487664510448365 1106 84-85 0.25114210121617475 1106 86-87 0.28447608314296247 1106 88-89 0.4677817641516775 1106 90-91 0.3312814016038068 1106 92-93 0.5250091414379803 1106 94-95 0.5265888336733227 1106 96-97 0.5650944700569589 1106 98-99 0.955881880899522 1106 100-101 1.4403052112787016 1107 1 0.038216029223807624 1107 2 0.02668460681525886 1107 3 -0.0651020950149821 1107 4 0.23747750601992124 1107 5 0.2342699574860987 1107 6 0.18571502915899885 1107 7 0.1329590211250533 1107 8 0.3018479385382662 1107 9 0.2797606453396213 1107 10-11 0.2805552881309694 1107 12-13 0.354617165547765 1107 14-15 0.10862384461935903 1107 16-17 0.13881179173198888 1107 18-19 0.3676638180318079 1107 20-21 0.5355983059636102 1107 22-23 0.5334714037106494 1107 24-25 0.4818698950896305 1107 26-27 0.270473135458424 1107 28-29 0.3614919182816365 1107 30-31 0.4529843622902021 1107 32-33 0.1762470077971301 1107 34-35 0.22384919531527459 1107 36-37 0.422199911726139 1107 38-39 0.19193259786318606 1107 40-41 0.18270767831949541 1107 42-43 0.31618530865745953 1107 44-45 0.34949277576824755 1107 46-47 0.3781374661594228 1107 48-49 0.33178884201917214 1107 50-51 0.04677880483939845 1107 52-53 0.3364022983096291 1107 54-55 0.34674457013129256 1107 56-57 0.20361691727058684 1107 58-59 0.2358963325604364 1107 60-61 0.14329360372666144 1107 62-63 0.1831521280836128 1107 64-65 0.1753954210581412 1107 66-67 0.21793404942115302 1107 68-69 0.36905336957230617 1107 70-71 0.4219230749942575 1107 72-73 0.2862972109456017 1107 74-75 0.17253495856840928 1107 76-77 0.08502671443804388 1107 78-79 0.12483178934314054 1107 80-81 0.17388412022233624 1107 82-83 0.3827480569045818 1107 84-85 0.3930620584316138 1107 86-87 0.41190482696353925 1107 88-89 0.39118025709290905 1107 90-91 0.31698182739247827 1107 92-93 0.1788443248836451 1107 94-95 0.2515907192109097 1107 96-97 0.26527993839021136 1107 98-99 0.5543209986050606 1107 100-101 0.9659756055960358 1108 1 0.012331981797437663 1108 2 0.06414832059400055 1108 3 0.1388958855779201 1108 4 0.21906600727834302 1108 5 0.1803993034803284 1108 6 0.042673445706263635 1108 7 0.037834552889883355 1108 8 0.040865556380325074 1108 9 0.13614367935029748 1108 10-11 -0.010381508881494028 1108 12-13 0.15628688854747708 1108 14-15 0.16575301985488977 1108 16-17 0.12676959206311977 1108 18-19 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-1.4146945619538087 2316 62-63 -1.4834439638622783 2316 64-65 -1.7249742119364662 2316 66-67 -1.8177541046372312 2316 68-69 -2.478659099919703 2316 70-71 -2.4956512937493933 2316 72-73 -1.9248718702523853 2316 74-75 -1.5447233398565423 2316 76-77 -1.3183201948439631 2316 78-79 -2.3626297440653126 2316 80-81 -1.7670910741950152 2316 82-83 -1.571304262713003 2316 84-85 -1.5381210198052635 2316 86-87 -2.335108548546188 2316 88-89 -2.7725727179094903 2316 90-91 -2.6500210377053754 2316 92-93 -2.7179050270585066 2316 94-95 -3.1796921574079704 2316 96-97 -4.3870485220871025 2316 98-99 -5.495620385636393 2316 100-101 -5.979325532863481 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3912.0 3 1470.0 4 1523.0 5 1759.0 6 1878.0 7 2080.0 8 2662.0 9 3352.0 10 4149.0 11 5261.0 12 6217.0 13 7252.0 14 8542.0 15 9963.0 16 11370.0 17 13755.0 18 12022.0 19 9901.0 20 10093.0 21 10893.0 22 12154.0 23 13623.0 24 15589.0 25 17903.0 26 20865.0 27 24701.0 28 29356.0 29 35292.0 30 42787.0 31 52259.0 32 65718.0 33 84484.0 34 112013.0 35 154816.0 36 217847.0 37 279976.0 38 206613.0 39 29214.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.39341023467094 17.60077419890119 14.500672821884992 23.505142744542884 2 15.67533655189131 19.031814207671523 40.14182135642192 25.151027884015253 3 19.93052202458302 22.75961074186534 29.369934572746097 27.939932660805546 4 13.006246333075094 15.823806081665861 37.0363791365344 34.13356844872464 5 14.30192234007999 36.14966647065726 34.4014657267724 15.146945462490349 6 32.97590885546156 34.84162573248788 17.32402300914674 14.858442402903826 7 29.25571848495968 31.52366294229844 20.47321994691576 18.74739862582612 8 26.595141936613096 33.47493501189558 19.768117257339927 20.161805794151395 9 25.74594453030193 16.922510449835602 20.49140157447647 36.840143445386 10-11 25.319172040901993 25.347697763486103 27.319732830675527 22.013397364936377 12-13 26.450108714395547 22.7580330019824 27.56269715423701 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22.730315620161896 25.517905966538546 28.628257994192168 23.123520419107386 40-41 22.920635599480573 25.642383925724904 28.475359569113213 22.96162090568131 42-43 22.732681954215114 25.598020007924895 28.097536446893983 23.571761590966013 44-45 22.849564179766006 25.477672130605782 28.242213862462695 23.430549827165517 46-47 23.41777319299046 25.781393769585065 27.87299419154677 22.92783884587771 48-49 23.225275318068466 25.855226645145134 27.60716677450113 23.312331262285273 50-51 22.764314829208267 26.248316478953598 28.00241824413574 22.984950447702392 52-53 23.26380541427421 26.108220684453322 27.450222489772987 23.177751411499482 54-55 22.78925726275926 25.906684547684026 27.72518919738576 23.578868992170953 56-57 23.05877427514406 25.952754139869803 27.516026269374834 23.472445315611303 58-59 23.44041466894302 25.727548351203623 27.40688369368234 23.425153286171017 60-61 22.978901684845336 25.866513642263765 28.128278689321363 23.026305983569532 62-63 22.786763185912733 25.982175778473167 28.01981750682519 23.21124352878891 64-65 22.56175673390256 26.248758320789644 27.735598303087013 23.453886642220787 66-67 22.66653015149296 26.00277091241817 27.501888963914634 23.828809972174234 68-69 22.776814117023374 25.843566805669283 27.84163357942057 23.53798549788677 70-71 22.983548788240302 25.803233476048316 27.490475767722135 23.722741967989247 72-73 22.895590059676802 25.787609684755246 27.85110704532281 23.46569321024514 74-75 22.684869884597465 26.47441334830648 27.38223064047791 23.45848612661814 76-77 22.870779135568263 25.95524421597995 27.423828817955453 23.75014783049634 78-79 22.83198703457759 26.0203352619248 27.51896001863682 23.62871768486079 80-81 23.18207371860819 26.09073632741936 27.2250457278299 23.502144226142548 82-83 23.06836672470763 26.07336360449285 27.51987314366661 23.338396527132907 84-85 22.93010616801013 26.26692615678543 27.24490708516901 23.558060590035424 86-87 22.927607822324866 26.387888531514253 27.25611773209992 23.42838591406096 88-89 22.783970989717965 26.262962706157495 26.93226376494725 24.020802539177293 90-91 22.84773940952681 26.062740935573608 27.18200392300948 23.9075157318901 92-93 23.021419780649936 26.040642561015364 27.528413564888954 23.409524093445746 94-95 22.74216281895504 25.845313892264077 27.602916160388823 23.809607128392063 96-97 22.461915817066316 25.865620306811977 27.890177577679097 23.782286298442614 98-99 22.547015820414845 26.187632926451066 27.243979969039056 24.021371284095036 100-101 22.485181785653168 25.89074581122917 27.408316350078042 24.215756053039613 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 73.0 1 57.5 2 60.0 3 99.5 4 90.0 5 77.0 6 163.5 7 359.0 8 591.5 9 746.0 10 736.0 11 680.5 12 699.0 13 795.5 14 912.5 15 1021.0 16 1159.5 17 1269.5 18 1425.5 19 1638.5 20 1842.0 21 2178.0 22 2599.0 23 3148.5 24 3906.5 25 4904.5 26 6417.5 27 8223.0 28 10135.5 29 12242.5 30 14879.5 31 18243.5 32 22284.0 33 24859.0 34 26264.0 35 28283.0 36 31260.5 37 34385.5 38 36544.5 39 38459.5 40 40607.5 41 43407.0 42 45672.5 43 47744.0 44 50356.5 45 53909.5 46 57014.5 47 62191.0 48 70317.5 49 69276.5 50 61097.0 51 57035.0 52 56469.5 53 57053.0 54 56816.5 55 53304.5 56 48595.5 57 44446.0 58 41294.5 59 36889.5 60 30505.0 61 25563.0 62 21528.5 63 16875.5 64 12334.0 65 9257.0 66 7132.0 67 5596.0 68 4324.5 69 3188.0 70 2373.5 71 1764.5 72 1309.5 73 1120.5 74 913.5 75 664.0 76 491.0 77 338.0 78 226.0 79 161.5 80 124.0 81 99.5 82 62.5 83 35.5 84 28.5 85 22.0 86 18.0 87 13.5 88 9.5 89 4.5 90 2.0 91 3.5 92 3.5 93 2.0 94 2.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03350022970660408 2 0.02241988293710834 3 0.021901504140874623 4 0.04976436443843701 5 0.05378180010924833 6 0.04898679624408644 7 0.05306902926442697 8 0.04425658972845375 9 0.04309023743692788 10-11 0.05219426504578257 12-13 0.04529334732092119 14-15 0.041632297072520544 16-17 0.048209228049735854 18-19 0.05290703589060393 20-21 0.012927071231078365 22-23 0.020897145223171792 24-25 0.006965715074390597 26-27 0.012441091109609252 28-29 0.0073868978463304934 30-31 0.008877236885502435 32-33 0.004600611816574255 34-35 0.009525210380794584 36-37 0.01020558255085134 38-39 0.011922712313375534 40-41 0.0023975019325809498 42-43 0.0022355085587579125 44-45 0.002170711209228698 46-47 3.239867476460743E-4 48-49 8.099668691151857E-4 50-51 2.915880728814669E-4 52-53 0.003628651573636032 54-55 0.007095309773449027 56-57 0.016814912202831257 58-59 0.010529569298497415 60-61 0.010626765322791238 62-63 0.005961356156687767 64-65 0.0 66-67 0.006414937603392271 68-69 0.01662052015424361 70-71 0.0067389243510383445 72-73 0.0028186847045208463 74-75 0.00427662506892818 76-77 0.00780808061827039 78-79 0.00592895748192316 80-81 0.011371934842377208 82-83 0.007127708448213634 84-85 0.016004945333716068 86-87 0.004827402539926507 88-89 0.006220545554804626 90-91 0.00453581446704504 92-93 0.01684731087759586 94-95 0.009590007730323799 96-97 0.009428014356500761 98-99 0.02342424185481117 100-101 0.03738807067835697 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1543273.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.32997535670067 #Duplication Level Percentage of deduplicated Percentage of total 1 81.98739726609767 53.5623464295419 2 11.055749626138216 14.445437012509247 3 3.122189067924867 6.119176045994755 4 1.3177597847397065 3.4435685705238486 5 0.6896130484459229 2.2526201730315685 6 0.415859846061235 1.6300868097013093 7 0.28732239047457175 1.3139535282392472 8 0.19764338157090014 1.0329629797953517 9 0.1514176962829122 0.890290293005794 >10 0.7158744514315623 8.23061455453072 >50 0.03682398566961374 1.6481856381822308 >100 0.018277091786908117 2.32507636410921 >500 0.002781126207999601 1.2670994049330717 >1k 0.001291237167999815 1.8385821959017592 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4001 0.2592541954663886 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3801 0.24629472556054566 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3583 0.23216890336317683 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3076 0.1993166471518649 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2571 0.1665939856396114 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2262 0.146571604635084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1682 0.1089891419081394 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1606 0.10406454334391906 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 6.479734952921485E-5 0.0 10-11 0.0 0.0 0.0 6.479734952921485E-5 0.0 12-13 0.0 0.0 0.0 6.479734952921485E-5 6.479734952921485E-5 14-15 0.0 0.0 0.0 6.479734952921485E-5 9.719602429382228E-5 16-17 0.0 0.0 0.0 6.479734952921485E-5 1.295946990584297E-4 18-19 0.0 0.0 0.0 6.479734952921485E-5 1.295946990584297E-4 20-21 0.0 0.0 0.0 1.295946990584297E-4 1.295946990584297E-4 22-23 0.0 0.0 0.0 2.26790723352252E-4 1.295946990584297E-4 24-25 0.0 0.0 0.0 3.239867476460743E-4 1.295946990584297E-4 26-27 0.0 0.0 0.0 5.507774709983263E-4 1.295946990584297E-4 28-29 0.0 0.0 0.0 0.0012635483158196896 1.295946990584297E-4 30-31 0.0 0.0 0.0 0.0027214886802270242 1.295946990584297E-4 32-33 0.0 0.0 0.0 0.003855442296988284 2.26790723352252E-4 34-35 0.0 0.0 0.0 0.004956997238984937 3.239867476460743E-4 36-37 0.0 0.0 0.0 0.006220545554804627 3.239867476460743E-4 38-39 0.0 0.0 0.0 0.00832645941450411 3.239867476460743E-4 40-41 0.0 0.0 0.0 0.0104647719489682 3.239867476460743E-4 42-43 0.0 0.0 0.0 0.016296533406597535 3.239867476460743E-4 44-45 0.0 0.0 0.0 0.02416941137439714 3.563854224106817E-4 46-47 0.0 0.0 0.0 0.03424539922619005 4.2118277193989653E-4 48-49 0.0 0.0 0.0 0.05122230480284434 4.859801214691114E-4 50-51 0.0 0.0 0.0 0.06852319712714472 5.183787962337188E-4 52-53 0.0 0.0 0.0 0.0863748669224434 5.183787962337188E-4 54-55 0.0 0.0 0.0 0.11627884373017607 5.183787962337188E-4 56-57 0.0 0.0 0.0 0.1488395118686065 5.183787962337188E-4 58-59 0.0 0.0 0.0 0.18742633351325397 5.183787962337188E-4 60-61 0.0 0.0 0.0 0.22896143456148071 5.183787962337188E-4 62-63 0.0 0.0 0.0 0.28912577359935665 5.183787962337188E-4 64-65 0.0 0.0 0.0 0.33937611815926283 5.183787962337188E-4 66-67 0.0 0.0 0.0 0.4139254687926245 5.507774709983263E-4 68-69 0.0 0.0 0.0 0.5141669685143199 5.831761457629338E-4 70-71 0.0 0.0 0.0 0.5769879988828936 5.831761457629338E-4 72-73 0.0 0.0 0.0 0.6692918232872602 6.155748205275411E-4 74-75 0.0 0.0 0.0 0.7781513704963412 6.479734952921486E-4 76-77 0.0 0.0 0.0 0.8642346493459032 6.479734952921486E-4 78-79 0.0 0.0 0.0 0.9465272832480061 6.479734952921486E-4 80-81 0.0 0.0 0.0 1.0693830579553976 6.803721700567561E-4 82-83 0.0 6.479734952921485E-5 0.0 1.2296917006906751 7.127708448213634E-4 84-85 0.0 6.479734952921485E-5 0.0 1.4343865278534647 7.127708448213634E-4 86-87 0.0 6.479734952921485E-5 0.0 1.7073777614200467 7.127708448213634E-4 88-89 0.0 6.479734952921485E-5 0.0 2.0330816388286452 7.127708448213634E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4680 0.0 23.859587 1 TACATGG 4665 0.0 23.223309 2 GTATAGA 485 0.0 22.533413 1 ACATGGG 4825 0.0 21.172983 3 GAGTACT 4625 0.0 21.012892 12-13 GTACTTT 4670 0.0 20.505459 14-15 GTATCAA 9445 0.0 20.274214 1 TAACGCA 190 8.223178E-6 20.008732 4 AGTACTT 4710 0.0 19.725597 12-13 AGAGTAC 6930 0.0 19.646017 10-11 CCGTATG 125 0.006019125 19.007061 9 ACCGTAT 125 0.006019125 19.007061 8 CGTACAC 150 7.705097E-4 19.006445 3 GGTATCA 7485 0.0 18.917572 1 GTAAGGT 665 0.0 18.579535 4 GTATTCG 130 0.0075494894 18.275429 1 GTCCTAA 550 0.0 18.142517 1 GTGTAGC 900 0.0 17.950533 1 TAAGGTG 770 0.0 17.89626 5 TCAACGC 10675 0.0 17.806364 4 >>END_MODULE