FastQCFastQC Report
Thu 26 May 2016
SRR936701_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936701_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543273
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69780.45215590501486125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37800.24493398122043217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32600.21123935946524042No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22000.14255416896427267No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20530.1330289585834781No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC18720.12130063831869022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17980.11650563445352831No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17920.11611685035635302No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16490.1068508293736753No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15980.10354616454768535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT9350.027.9392434
TAAGGTG10100.026.805945
GGTAAGG10350.025.23983
AGGTAAG9500.024.9982682
AAGGTAA9950.023.8785251
CCGTATG1000.001653008923.7522039
GTACATG52050.022.3669071
GTACCGT1754.0497507E-621.7148936
TACATGG52000.021.55622
GAGTACT49750.020.86306812-13
GTACTTT51600.020.16044814-15
ACATGGG50800.020.1019543
GGTGTGC15850.020.0794268
AGTACTT50550.019.828112-13
AGAGTAC76100.019.82015610-11
AAGGTGT14400.019.7922216
GTATAGC3850.019.7478491
TAGAGCG1250.00603334418.99935
TAGCCCT8300.018.3119854
TACCGTA1300.00756305118.2697437
Produced by FastQC (version 0.11.5)