##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936701_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1543273 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.943816162143705 31.0 31.0 34.0 30.0 34.0 2 32.096719763774786 33.0 31.0 34.0 30.0 34.0 3 32.20713963116053 33.0 31.0 34.0 30.0 34.0 4 35.92895877787015 37.0 35.0 37.0 35.0 37.0 5 35.70638700994574 37.0 35.0 37.0 33.0 37.0 6 35.81176629151161 37.0 35.0 37.0 35.0 37.0 7 35.761515946951704 37.0 35.0 37.0 33.0 37.0 8 35.733752226598924 37.0 35.0 37.0 33.0 37.0 9 37.38523968215604 39.0 37.0 39.0 34.0 39.0 10-11 37.25197712912751 39.0 37.0 39.0 33.5 39.0 12-13 37.16791228771578 39.0 37.0 39.0 33.0 39.0 14-15 38.30474938653109 40.0 38.0 41.0 33.0 41.0 16-17 38.230193556162774 40.0 38.0 41.0 33.0 41.0 18-19 38.3494177634158 40.0 38.0 41.0 33.5 41.0 20-21 38.36204676683905 40.0 38.0 41.0 34.0 41.0 22-23 38.274362021495875 40.0 38.0 41.0 34.0 41.0 24-25 38.147970255424674 40.0 37.5 41.0 33.0 41.0 26-27 37.931414921404055 40.0 37.0 41.0 33.0 41.0 28-29 37.86684468658494 40.0 37.0 41.0 33.0 41.0 30-31 37.87579579244891 40.0 37.0 41.0 33.0 41.0 32-33 37.75757108431237 40.0 37.0 41.0 33.0 41.0 34-35 37.6012526623611 40.0 37.0 41.0 32.5 41.0 36-37 37.355417350008715 40.0 36.5 41.0 31.5 41.0 38-39 37.189879885153175 39.0 36.0 41.0 31.0 41.0 40-41 36.98660379595833 39.0 36.0 41.0 31.0 41.0 42-43 36.76112197906657 39.0 35.5 41.0 31.0 41.0 44-45 36.53695004059554 39.0 35.0 40.0 30.0 41.0 46-47 36.23422071143602 39.0 35.0 40.0 30.0 41.0 48-49 35.90766928469558 39.0 35.0 40.0 29.0 41.0 50-51 35.59192475990962 38.0 35.0 40.0 28.0 41.0 52-53 35.31539170321777 38.0 34.0 40.0 27.5 41.0 54-55 35.04312328408518 38.0 34.0 40.0 27.0 41.0 56-57 34.707479817245556 38.0 34.0 40.0 26.0 41.0 58-59 34.34754933184213 37.0 33.0 40.0 26.0 41.0 60-61 33.96210780594231 37.0 33.0 39.5 25.5 41.0 62-63 33.55068157092102 36.0 32.5 39.0 24.5 40.0 64-65 33.13310412350893 36.0 32.0 39.0 23.0 40.0 66-67 32.65472699904683 35.0 31.5 38.5 22.0 40.0 68-69 32.35156709149969 35.0 31.0 38.0 22.0 40.0 70-71 31.90153491961565 35.0 31.0 37.5 20.5 39.5 72-73 31.441286473618085 34.5 30.5 37.0 20.0 39.0 74-75 30.91774721646786 34.0 30.0 36.0 18.0 39.0 76-77 29.527123198552687 32.5 28.5 35.0 17.5 37.0 78-79 30.31270261321231 34.0 30.0 35.0 18.0 37.0 80-81 30.114017416231604 34.0 30.0 35.0 15.5 37.0 82-83 29.784842992782224 34.0 29.5 35.0 11.5 36.5 84-85 29.369253852040437 34.0 29.0 35.0 7.0 36.0 86-87 29.0067923821644 34.0 29.0 35.0 4.5 36.0 88-89 28.601046282802848 33.0 29.0 35.0 2.0 35.0 90-91 28.11244122070431 33.0 27.5 35.0 2.0 35.0 92-93 27.603238053150672 33.0 27.0 35.0 2.0 35.0 94-95 27.006013194036314 32.5 25.5 34.0 2.0 35.0 96-97 26.21316092486553 32.0 25.0 34.0 2.0 35.0 98-99 25.23550629085068 32.0 21.5 34.0 2.0 35.0 100-101 21.95176679693094 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30473390269323275 1101 2 0.3369625416024249 1101 3 0.24758362937944867 1101 4 0.14524883366149055 1101 5 0.11121326019090105 1101 6 0.16646997459965007 1101 7 0.14799111428829548 1101 8 0.15907239101657922 1101 9 0.2900483737926578 1101 10-11 0.28950021901604117 1101 12-13 0.244680174516283 1101 14-15 0.32108940175584166 1101 16-17 0.39191923616306923 1101 18-19 0.31469480025081253 1101 20-21 0.27104698573828045 1101 22-23 0.19741472218903056 1101 24-25 0.16264994007016043 1101 26-27 0.20108589243237418 1101 28-29 0.16390127789281905 1101 30-31 0.08651416430546988 1101 32-33 0.2703185732083213 1101 34-35 0.18419659260806753 1101 36-37 0.2264162759019399 1101 38-39 0.25944774047830776 1101 40-41 0.2883313285653415 1101 42-43 0.40396828294817055 1101 44-45 0.5000739405117898 1101 46-47 0.46398912928466274 1101 48-49 0.4175215924428457 1101 50-51 0.4156457159415723 1101 52-53 0.3882692395600813 1101 54-55 0.46247201141041216 1101 56-57 0.3521906180553813 1101 58-59 0.3748356492563687 1101 60-61 0.17029776781127737 1101 62-63 0.21061629112710278 1101 64-65 0.2732353953434341 1101 66-67 0.22868948360702746 1101 68-69 0.13085415535451972 1101 70-71 0.2439499278366526 1101 72-73 0.15388311819963718 1101 74-75 0.32046117105385363 1101 76-77 0.05813264312947197 1101 78-79 0.020882359133270967 1101 80-81 0.07948562553357519 1101 82-83 0.31187577051616344 1101 84-85 0.37585322977330904 1101 86-87 0.3653453233913453 1101 88-89 0.0915508239841678 1101 90-91 0.011800724410466756 1101 92-93 -0.05930808454936454 1101 94-95 0.07045773029552649 1101 96-97 0.10584664728776616 1101 98-99 0.21934374102036003 1101 100-101 0.6863052942976076 1102 1 0.2902751377372148 1102 2 0.3325763048852082 1102 3 0.2762195930177924 1102 4 0.15380036513951723 1102 5 0.06558739898879651 1102 6 0.19001160949468243 1102 7 0.16096795148802556 1102 8 0.1487767652900942 1102 9 0.2109780975451585 1102 10-11 0.20242712872244795 1102 12-13 0.19783859367119305 1102 14-15 0.3372949553744178 1102 16-17 0.3032398709023667 1102 18-19 0.25066326159486607 1102 20-21 0.17219768916897493 1102 22-23 0.1716128780242414 1102 24-25 0.20693330944577326 1102 26-27 -0.04979598930331974 1102 28-29 0.04605757438829983 1102 30-31 0.2646922661554072 1102 32-33 0.18873642941391466 1102 34-35 0.07091938212421667 1102 36-37 0.317941616058242 1102 38-39 0.2345099949161522 1102 40-41 0.20256772352549746 1102 42-43 0.28301387836249603 1102 44-45 0.28418306751623135 1102 46-47 0.3151554872612863 1102 48-49 0.3056937073046342 1102 50-51 0.1296433588681154 1102 52-53 0.04247177112379319 1102 54-55 0.19643661004241153 1102 56-57 0.3838951151941856 1102 58-59 0.13387823770455753 1102 60-61 0.26791935459485217 1102 62-63 0.23256283287354762 1102 64-65 0.1492499096133244 1102 66-67 0.06436498130572943 1102 68-69 -0.027728819936093885 1102 70-71 -0.021338177780066303 1102 72-73 0.01859214033748202 1102 74-75 -0.11375376559238859 1102 76-77 -0.21051014276537572 1102 78-79 -0.19942816131436558 1102 80-81 0.2625473529440079 1102 82-83 0.0564955644365952 1102 84-85 0.11783842558446267 1102 86-87 0.12693440260765954 1102 88-89 -0.010389773884767806 1102 90-91 0.14836117547694272 1102 92-93 0.19706555200973952 1102 94-95 0.34430666311893177 1102 96-97 0.3670444975764191 1102 98-99 0.31937979228105107 1102 100-101 0.5989218074994263 1103 1 0.11248925939161225 1103 2 0.082090498189622 1103 3 0.1637465436251233 1103 4 0.1375031262022972 1103 5 -0.07638275248030624 1103 6 0.047279392257095765 1103 7 0.06167812147807439 1103 8 0.11535037243960033 1103 9 0.16315649242128671 1103 10-11 0.09923065436120027 1103 12-13 0.12439719961197682 1103 14-15 0.1616508010709623 1103 16-17 0.2707247919318334 1103 18-19 0.10397460516074375 1103 20-21 0.17718044787434195 1103 22-23 0.032607361573930405 1103 24-25 0.028896956207411506 1103 26-27 0.01854695830049735 1103 28-29 0.02910140640314296 1103 30-31 0.0019522891074146287 1103 32-33 0.09702755763686355 1103 34-35 0.11173546643220789 1103 36-37 0.08321580966824627 1103 38-39 0.22111586366840186 1103 40-41 0.29287579127268515 1103 42-43 0.11831735952694089 1103 44-45 0.041535515157967495 1103 46-47 0.07475170427186129 1103 48-49 0.011307537229463094 1103 50-51 0.09208835943837101 1103 52-53 -0.018934959174771393 1103 54-55 -0.05584290247560375 1103 56-57 -0.09815554840056961 1103 58-59 -0.02939510352682362 1103 60-61 -0.15364994427736178 1103 62-63 0.0031118694728959895 1103 64-65 -0.08945730160795762 1103 66-67 0.17459480702649444 1103 68-69 0.08146704651877457 1103 70-71 0.09399746130470987 1103 72-73 0.0034572358699165306 1103 74-75 -0.12750996675591963 1103 76-77 -0.2985597073265076 1103 78-79 -0.15942025416579142 1103 80-81 0.07817941460339384 1103 82-83 0.047758001551848395 1103 84-85 0.07682138838048402 1103 86-87 -0.0930368500778549 1103 88-89 -0.04968936641286703 1103 90-91 -0.03721329878498736 1103 92-93 0.016392664034228233 1103 94-95 -0.10927853845332791 1103 96-97 -0.015067365929500198 1103 98-99 0.2292037831919238 1103 100-101 0.18286309269210577 1104 1 0.18902832665959224 1104 2 0.26379106666105656 1104 3 0.2145581068872886 1104 4 0.15512985510290633 1104 5 0.07626965706599265 1104 6 0.16973618545647184 1104 7 0.1972472443588842 1104 8 0.19340033483865682 1104 9 0.1947487153114622 1104 10-11 0.20419500775065558 1104 12-13 0.18714734875857886 1104 14-15 0.3070590284778234 1104 16-17 0.3075440033862904 1104 18-19 0.2845551454714723 1104 20-21 0.16427326620806326 1104 22-23 0.2422280606086531 1104 24-25 0.26653807404122887 1104 26-27 0.4105536716121492 1104 28-29 0.38786385528756995 1104 30-31 0.17579294567249804 1104 32-33 0.3591495767047732 1104 34-35 0.254369338369564 1104 36-37 0.33557729761422905 1104 38-39 0.3064514378199732 1104 40-41 0.34027412585937356 1104 42-43 0.43418101552744304 1104 44-45 0.4322896255470354 1104 46-47 0.3634543916537112 1104 48-49 0.4200398139266994 1104 50-51 0.2749252421942785 1104 52-53 0.34842970599395784 1104 54-55 0.23607529432476326 1104 56-57 0.2857126745718972 1104 58-59 0.10016243963425353 1104 60-61 0.20099382962324341 1104 62-63 0.33745141858155847 1104 64-65 0.3966654923365809 1104 66-67 0.25943351154249683 1104 68-69 0.35976175792865916 1104 70-71 0.21684483575297975 1104 72-73 0.12892267614384423 1104 74-75 0.14045305631437444 1104 76-77 0.09039578641162294 1104 78-79 0.11784728856099846 1104 80-81 0.2633617849029939 1104 82-83 0.11612714911898436 1104 84-85 0.29277138940908287 1104 86-87 0.2166032208725035 1104 88-89 0.5023479419537438 1104 90-91 0.6280057124358684 1104 92-93 0.5173378306555563 1104 94-95 0.5116436447329882 1104 96-97 0.7754599387570202 1104 98-99 1.025036078403918 1104 100-101 1.391778059696989 1105 1 0.09259299417928091 1105 2 0.14353035393207136 1105 3 0.10238554768174879 1105 4 0.03280028793256662 1105 5 -0.027508926295993774 1105 6 0.017694238266265927 1105 7 0.03222398652350478 1105 8 0.12431772510372241 1105 9 0.14692379267779643 1105 10-11 0.11681514766836187 1105 12-13 -0.0536242949612884 1105 14-15 0.21954367344910963 1105 16-17 0.1771262826925053 1105 18-19 0.18365327321252778 1105 20-21 0.20413466510390066 1105 22-23 0.2558024812079722 1105 24-25 0.15069964153880733 1105 26-27 0.2537216265460174 1105 28-29 0.20244269608995324 1105 30-31 0.2676880789752403 1105 32-33 0.35687012988206135 1105 34-35 0.2675538914343676 1105 36-37 0.16402040315839628 1105 38-39 0.3495300427844583 1105 40-41 0.4693790794779247 1105 42-43 0.3141473527027756 1105 44-45 0.3083425874537866 1105 46-47 0.28363796174102873 1105 48-49 0.234546433366134 1105 50-51 0.2858659797725167 1105 52-53 0.2556073590425214 1105 54-55 0.41642545928347374 1105 56-57 0.2284224392836407 1105 58-59 0.45687397728459445 1105 60-61 0.42151632881474654 1105 62-63 0.5948418050872775 1105 64-65 0.49002933972836615 1105 66-67 0.6363296418835205 1105 68-69 0.5840616580970064 1105 70-71 0.5517185186717981 1105 72-73 0.6203957618622908 1105 74-75 0.47144883551896655 1105 76-77 0.12544583645527396 1105 78-79 0.42335164293396943 1105 80-81 0.4803077288106401 1105 82-83 0.37992494464177184 1105 84-85 0.5141352994353277 1105 86-87 0.36197891256781034 1105 88-89 0.3494661817465641 1105 90-91 0.48295230058363714 1105 92-93 0.5376646291272777 1105 94-95 0.4581670808848308 1105 96-97 0.45892488966566347 1105 98-99 0.8853776982332171 1105 100-101 0.8975238123778091 1106 1 0.033477016218032674 1106 2 0.1321914155424011 1106 3 0.12861717038720855 1106 4 0.016978624592383085 1106 5 -0.07494011663870737 1106 6 0.042487496903447663 1106 7 0.07612157418245147 1106 8 0.04819607084506572 1106 9 0.0425398037558935 1106 10-11 0.1339430490634328 1106 12-13 0.017946181463948108 1106 14-15 0.24049759595293096 1106 16-17 0.15473058201153123 1106 18-19 -0.01964274163474755 1106 20-21 0.006342876581996393 1106 22-23 -0.03997559761833713 1106 24-25 0.06572110604129477 1106 26-27 -0.10786408363301803 1106 28-29 -0.07727848729480513 1106 30-31 0.13620289381786677 1106 32-33 -0.002992650254107332 1106 34-35 -0.07174485743129821 1106 36-37 0.004377867185645812 1106 38-39 0.23719578923497409 1106 40-41 0.1837199072354494 1106 42-43 0.269060289512403 1106 44-45 0.37116406848055306 1106 46-47 0.3066543474151544 1106 48-49 0.31881363927991657 1106 50-51 0.24415273897064083 1106 52-53 0.09787672403876257 1106 54-55 0.20162577529339387 1106 56-57 0.3464360629436882 1106 58-59 0.2566173728616832 1106 60-61 0.25698325258657206 1106 62-63 0.15424064196867704 1106 64-65 0.11848006491923257 1106 66-67 0.19514244697682415 1106 68-69 0.30002179769309123 1106 70-71 0.17771979145158667 1106 72-73 0.15635435145294707 1106 74-75 0.20191040255396686 1106 76-77 0.021815341361691054 1106 78-79 0.1866313315126149 1106 80-81 0.18599583903992922 1106 82-83 0.44827199017437636 1106 84-85 0.4013399704825211 1106 86-87 0.18019093009448284 1106 88-89 0.12383933428690241 1106 90-91 0.1635096675136225 1106 92-93 0.19308869217631042 1106 94-95 0.47758089887595645 1106 96-97 0.30990757262263813 1106 98-99 0.7760744905413652 1106 100-101 0.9264915019342546 1107 1 -0.012135852061739172 1107 2 0.00645622729717843 1107 3 -0.03497275604254213 1107 4 -0.0029443083441904605 1107 5 -0.05558353062734511 1107 6 -0.006801826839925695 1107 7 -0.02909759384979793 1107 8 -0.050305783838261675 1107 9 0.058414658912326445 1107 10-11 -0.04685429335705038 1107 12-13 0.089036090752451 1107 14-15 -0.04120240473271508 1107 16-17 -0.003600408684199863 1107 18-19 -0.03707265106037738 1107 20-21 0.09331484035225657 1107 22-23 0.016087948426495302 1107 24-25 -0.025127523001472696 1107 26-27 -0.06455671358083492 1107 28-29 -0.11305227763673997 1107 30-31 -0.08655383069481104 1107 32-33 0.006061358398802952 1107 34-35 0.048219214507106756 1107 36-37 0.1399192221672294 1107 38-39 -0.09161008267187754 1107 40-41 -0.03185874710779757 1107 42-43 -0.05023253104612735 1107 44-45 0.07850155228207001 1107 46-47 0.1296880745401623 1107 48-49 0.023372869157803677 1107 50-51 0.13374354303243052 1107 52-53 0.055264603117514355 1107 54-55 0.06847013959296788 1107 56-57 -0.26101523876289434 1107 58-59 -0.030128669223515203 1107 60-61 -0.07254837987758833 1107 62-63 0.014865132016460336 1107 64-65 -0.08649466887714397 1107 66-67 -0.0628251492027303 1107 68-69 -0.1251350447513886 1107 70-71 -0.3112145027059867 1107 72-73 -0.12111518854444725 1107 74-75 -0.25867637674214095 1107 76-77 -0.2748181169885022 1107 78-79 -0.08931812236713554 1107 80-81 0.0953837592576896 1107 82-83 0.00801576275797089 1107 84-85 -0.03426720690199758 1107 86-87 -0.06784433974308612 1107 88-89 0.05641422441148336 1107 90-91 -0.03195270058445132 1107 92-93 0.00898000293347323 1107 94-95 0.46926567813092745 1107 96-97 0.5432531333815156 1107 98-99 0.9697809089113001 1107 100-101 1.1969629548952305 1108 1 -0.03405607063286098 1108 2 -0.01657837732221168 1108 3 -0.04602455531941274 1108 4 -0.04186021340235868 1108 5 -0.0896120250554091 1108 6 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-0.6881075273931003 2316 96-97 -0.36024688430427076 2316 98-99 -0.7596277793589756 2316 100-101 -1.1236508430522036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 12.0 5 54.0 6 86.0 7 215.0 8 579.0 9 1014.0 10 1681.0 11 2659.0 12 3672.0 13 5115.0 14 6545.0 15 8462.0 16 10565.0 17 13196.0 18 12266.0 19 9484.0 20 8910.0 21 9518.0 22 10145.0 23 11335.0 24 13328.0 25 15871.0 26 19642.0 27 24613.0 28 30273.0 29 37567.0 30 46234.0 31 57398.0 32 72281.0 33 93014.0 34 122704.0 35 168976.0 36 241171.0 37 305394.0 38 170585.0 39 8701.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22356693265572 19.02675231903129 14.814834021534093 22.934846726778897 2 16.1365910304463 20.42949086818354 38.62171262383552 24.812205477534643 3 20.488058703383068 23.39931675151113 29.196236949331784 26.916387595774022 4 13.75315240126796 16.711096193272976 36.21237529709681 33.323376108362254 5 14.387086030277485 37.191294526712625 33.475845596882955 14.945773846126931 6 33.07438102211538 35.913158423347824 16.42888153077556 14.583579023761233 7 29.698180425627868 32.024794057823854 19.76325640376006 18.51376911278821 8 27.015310965720257 34.26969823226351 18.791425755520898 19.923565046495337 9 26.127500309410152 17.40278203464227 19.65405677206483 36.81566088388274 10-11 25.620146650216686 26.063265909138032 26.560718062496115 21.75586937814917 12-13 26.745638354294996 23.296788246389852 26.920204619655813 23.037368779659335 14-15 23.05929275076827 25.30260346383322 25.369216887280434 26.268886898118073 16-17 22.89738142441362 28.204418734928066 26.01313029957728 22.88506954108103 18-19 22.847675038700217 27.07207344390785 28.075039218595805 22.00521229879613 20-21 23.52522852405245 25.833310114283087 28.19864016282278 22.442821198841685 22-23 23.097257339400198 25.732870844959262 28.21436115823401 22.95551065740653 24-25 23.4611127886588 26.040201962342007 28.06659677124628 22.43208847775291 26-27 22.95623475772251 26.072260909882356 28.35411105304272 22.61739327935241 28-29 22.672742436230664 26.95131284186734 27.907358770434275 22.468585951467716 30-31 22.970564498231173 26.177097700283593 27.8614267047809 22.990911096704338 32-33 22.922231164297017 26.215344600347574 27.98201994989846 22.880404285456947 34-35 23.5406894214341 26.1764933646419 27.786657611504378 22.49615960241962 36-37 23.700323993502632 25.856392366856944 27.96583341244966 22.47745022719076 38-39 23.181450277648352 26.35977705184044 27.862655276031518 22.596117394479695 40-41 23.375305525244226 26.32305714456042 27.62628592756557 22.675351402629783 42-43 23.164865940155487 26.48898504859175 27.23522710926049 23.110921901992267 44-45 23.31575363014715 26.261666073764772 27.404055189770727 23.01852510631735 46-47 23.976477266173905 26.7230749193435 26.846092687424715 22.45435512705788 48-49 23.85783364787261 26.725233069552328 26.63289621823896 22.784037064336108 50-51 23.235276774669707 27.09179081411481 27.075137744215326 22.59779466700016 52-53 23.79725829094671 27.058719851066055 26.444360853778214 22.69966100420902 54-55 23.296168809793496 26.970966052974678 26.69634687810226 23.036518259129565 56-57 23.66156953475239 26.988696283533457 26.57194686420604 22.777787317508107 58-59 23.941063664966045 26.61342866408965 26.37364381923054 23.07186385171376 60-61 23.313320934934325 26.954951043603913 27.177279825817614 22.554448195644145 62-63 23.116466373530525 27.036057948550297 27.09771568299954 22.749759994919636 64-65 23.020095759513172 27.410666522371805 26.706030218324823 22.8632074997902 66-67 23.262071588764094 26.958277795093537 26.531608035349212 23.248042580793154 68-69 23.265913651918492 26.87428031925475 26.724658003821883 23.135148025004877 70-71 23.552832194951897 26.951064393662044 26.384703160101942 23.111400251284124 72-73 23.487028058893742 26.77193773675623 26.723727915171448 23.01730628917858 74-75 23.303654454909815 27.644773633750972 26.185000502208094 22.866571409131115 76-77 23.582750791194606 26.8936507442802 26.289919364245 23.23367910028019 78-79 23.533210170701853 27.19594846350189 26.3345984394936 22.936242926302654 80-81 23.795351019327715 27.23444197230622 26.12878382139433 22.841423186971735 82-83 23.62749071817766 27.317312773517706 26.276232346393495 22.778964161911137 84-85 23.329139651547486 27.71802258237701 26.087962340456762 22.86487542561874 86-87 23.406279949378867 27.636727925667543 25.862201915593864 23.094790209359726 88-89 23.20666150617461 27.658962974387517 25.639599140580422 23.49477637885745 90-91 23.39279158483583 27.393934759342 25.907354260641842 23.30591939518033 92-93 23.58409935344576 27.374359957959072 26.163966102881048 22.877574585714118 94-95 23.260794412795672 27.302290213353007 26.175732168770843 23.261183205080478 96-97 23.19265241800453 27.33503061060511 26.433657150855076 23.038659820535283 98-99 23.071813896223816 27.63359199079849 25.727680668729448 23.566913444248247 100-101 22.81381189997055 27.528122469755324 25.865641354958886 23.792424275315245 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 121.0 1 91.0 2 76.0 3 96.5 4 95.0 5 106.0 6 162.5 7 293.0 8 480.0 9 616.0 10 629.0 11 633.5 12 718.0 13 823.5 14 923.5 15 1095.0 16 1267.5 17 1402.5 18 1570.5 19 1715.0 20 1948.5 21 2311.5 22 2739.0 23 3237.5 24 3953.0 25 4992.5 26 6352.5 27 8219.5 28 10213.0 29 12624.0 30 15639.5 31 18573.0 32 21918.5 33 24463.0 34 26415.5 35 28630.0 36 31798.5 37 35090.0 38 37071.0 39 38630.5 40 40346.5 41 43335.5 42 45572.5 43 47330.5 44 50039.5 45 53261.0 46 55827.0 47 60912.0 48 69840.0 49 68918.5 50 60676.0 51 56922.5 52 55721.5 53 56299.5 54 56785.0 55 53881.5 56 48800.5 57 44728.5 58 41676.0 59 37195.0 60 30820.5 61 25480.0 62 21802.0 63 17191.5 64 12371.0 65 9169.0 66 7161.5 67 5702.0 68 4276.0 69 3212.0 70 2488.5 71 1794.5 72 1364.0 73 1172.0 74 930.5 75 712.0 76 561.5 77 383.0 78 276.0 79 201.0 80 148.0 81 112.0 82 73.5 83 52.5 84 31.0 85 19.0 86 9.5 87 3.5 88 4.5 89 3.5 90 2.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.039072801766116556 2 0.0062853429043338415 3 0.0036934489231652467 4 3.239867476460743E-4 5 9.07162893409008E-4 6 1.9439204858764456E-4 7 0.0 8 0.0 9 9.07162893409008E-4 10-11 5.831761457629338E-4 12-13 0.0012635483158196898 14-15 0.0026242926559332017 16-17 0.0031426714521669208 18-19 0.0 20-21 0.0 22-23 0.001749528437288801 24-25 9.719602429382229E-4 26-27 0.004762605190397292 28-29 0.0060909508557461965 30-31 0.00113395361676126 32-33 4.53581446704504E-4 34-35 0.008974432909796257 36-37 0.01208470568719857 38-39 0.008682844836914791 40-41 0.001879123136347231 42-43 4.53581446704504E-4 44-45 0.0010367575924674379 46-47 0.0 48-49 6.80372170056756E-4 50-51 9.07162893409008E-4 52-53 0.002494697956874772 54-55 0.0029158807288146684 56-57 0.0012635483158196898 58-59 9.395615681736155E-4 60-61 0.004082233020340536 62-63 0.004956997238984936 64-65 0.008844838210737829 66-67 0.002721488680227024 68-69 0.003499056874577602 70-71 0.0 72-73 0.0012311496410550822 74-75 0.005702166758570907 76-77 0.0023975019325809498 78-79 0.003628651573636032 80-81 0.004827402539926507 82-83 0.004438618442751218 84-85 0.005086591938043367 86-87 0.0028510833792854537 88-89 0.008974432909796257 90-91 0.013056665930136793 92-93 9.719602429382229E-4 94-95 0.0021059138596994827 96-97 0.0011015549419966526 98-99 0.003110272777402313 100-101 0.004373821093222003 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1543273.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.706152039098114 #Duplication Level Percentage of deduplicated Percentage of total 1 80.44582464658777 50.444481111995586 2 11.740986130550219 14.72464122782449 3 3.5215269215592966 6.624642076592233 4 1.4968570010973279 3.7544857076639 5 0.7691739208914504 2.411596841396426 6 0.45195190492162485 1.7004098918625254 7 0.29750706540502425 1.3058866293195353 8 0.22575358413304075 1.1324910856014225 9 0.1616289335262765 0.912161563165438 >10 0.8203571212356524 9.100437787583532 >50 0.04326979522376072 1.8737606009703713 >100 0.020917566685121104 2.5551427534978686 >500 0.0020709308211917573 0.8668811171499667 >1k 0.0020709308211917573 2.139899706232601 >5k 1.0354654105958788E-4 0.45308189914406916 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6978 0.45215590501486125 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3780 0.24493398122043217 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3260 0.21123935946524042 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2200 0.14255416896427267 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2053 0.1330289585834781 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1872 0.12130063831869022 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1798 0.11650563445352831 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1792 0.11611685035635302 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1649 0.1068508293736753 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1598 0.10354616454768535 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.479734952921485E-5 7 0.0 0.0 0.0 0.0 6.479734952921485E-5 8 0.0 0.0 0.0 0.0 6.479734952921485E-5 9 0.0 0.0 0.0 0.0 1.295946990584297E-4 10-11 0.0 0.0 0.0 0.0 1.295946990584297E-4 12-13 0.0 0.0 0.0 0.0 1.295946990584297E-4 14-15 0.0 0.0 0.0 0.0 1.295946990584297E-4 16-17 0.0 0.0 0.0 0.0 1.295946990584297E-4 18-19 0.0 0.0 0.0 0.0 1.295946990584297E-4 20-21 0.0 0.0 0.0 6.479734952921485E-5 1.295946990584297E-4 22-23 0.0 0.0 0.0 1.6199337382303712E-4 1.295946990584297E-4 24-25 0.0 0.0 0.0 3.239867476460743E-4 1.295946990584297E-4 26-27 0.0 0.0 0.0 5.507774709983263E-4 1.295946990584297E-4 28-29 0.0 0.0 0.0 0.0012311496410550822 1.295946990584297E-4 30-31 0.0 0.0 0.0 0.0026890900054624164 1.295946990584297E-4 32-33 0.0 0.0 0.0 0.003920239646517498 1.295946990584297E-4 34-35 0.0 0.0 0.0 0.005021794588514152 1.295946990584297E-4 36-37 0.0 0.0 0.0 0.006252944229569233 1.295946990584297E-4 38-39 0.0 0.0 0.0 0.00842365543879793 1.295946990584297E-4 40-41 0.0 0.0 0.0 0.010529569298497413 1.295946990584297E-4 42-43 0.0 0.0 0.0 0.01639372943089136 1.295946990584297E-4 44-45 0.0 0.0 0.0 0.024428600772514 1.295946990584297E-4 46-47 0.0 0.0 0.0 0.0344397912747777 1.295946990584297E-4 48-49 0.0 0.0 0.0 0.05109271010378591 1.295946990584297E-4 50-51 0.0 0.0 0.0 0.06865279182620315 1.295946990584297E-4 52-53 0.0 0.0 0.0 0.08673125234485408 1.295946990584297E-4 54-55 0.0 3.239867476460743E-5 0.0 0.11640843842923448 1.295946990584297E-4 56-57 0.0 6.479734952921485E-5 0.0 0.1492282959657818 1.295946990584297E-4 58-59 0.0 6.479734952921485E-5 0.0 0.18794471230948767 1.295946990584297E-4 60-61 0.0 1.9439204858764456E-4 0.0 0.2293178199838914 1.295946990584297E-4 62-63 0.0 1.9439204858764456E-4 0.0 0.2892877669731797 1.295946990584297E-4 64-65 0.0 1.9439204858764456E-4 0.0 0.33937611815926283 1.295946990584297E-4 66-67 0.0 1.9439204858764456E-4 0.0 0.4138282727683307 1.295946990584297E-4 68-69 0.0 1.9439204858764456E-4 0.0 0.5145881512862598 1.295946990584297E-4 70-71 0.0 1.9439204858764456E-4 0.0 0.5777655670772442 1.295946990584297E-4 72-73 0.0 1.9439204858764456E-4 0.0 0.6709117570254906 1.295946990584297E-4 74-75 0.0 1.9439204858764456E-4 0.0 0.7805164737541576 1.295946990584297E-4 76-77 0.0 1.9439204858764456E-4 0.0 0.8668589420018363 1.6199337382303712E-4 78-79 0.0 1.9439204858764456E-4 0.0 0.9491515759039393 1.9439204858764456E-4 80-81 0.0 1.9439204858764456E-4 0.0 1.0728821148299752 1.9439204858764456E-4 82-83 0.0 1.9439204858764456E-4 0.0 1.2324779867204312 1.9439204858764456E-4 84-85 0.0 1.9439204858764456E-4 0.0 1.4367192324365163 1.9439204858764456E-4 86-87 0.0 1.9439204858764456E-4 0.0 1.7074749574443406 1.9439204858764456E-4 88-89 0.0 1.9439204858764456E-4 0.0 2.0344747818435236 1.9439204858764456E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 935 0.0 27.939243 4 TAAGGTG 1010 0.0 26.80594 5 GGTAAGG 1035 0.0 25.2398 3 AGGTAAG 950 0.0 24.998268 2 AAGGTAA 995 0.0 23.878525 1 CCGTATG 100 0.0016530089 23.752203 9 GTACATG 5205 0.0 22.366907 1 GTACCGT 175 4.0497507E-6 21.714893 6 TACATGG 5200 0.0 21.5562 2 GAGTACT 4975 0.0 20.863068 12-13 GTACTTT 5160 0.0 20.160448 14-15 ACATGGG 5080 0.0 20.101954 3 GGTGTGC 1585 0.0 20.079426 8 AGTACTT 5055 0.0 19.8281 12-13 AGAGTAC 7610 0.0 19.820156 10-11 AAGGTGT 1440 0.0 19.792221 6 GTATAGC 385 0.0 19.747849 1 TAGAGCG 125 0.006033344 18.9993 5 TAGCCCT 830 0.0 18.311985 4 TACCGTA 130 0.007563051 18.269743 7 >>END_MODULE