FastQCFastQC Report
Thu 26 May 2016
SRR936700_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936700_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566792
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT40770.26021322549515186No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT37200.23742781428549545No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36920.23564072321022828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28970.18490010160889256No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26150.16690154149370176No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23470.14979652691614456No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15810.10090682107133557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15750.10052387298377832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG46800.024.6757181
TACATGG48500.023.2243582
ACATGGG48900.022.0617643
GTGTAGC9150.021.8141161
GTACTTT49400.021.01904914-15
GAGTACT49200.020.76941712-13
GTCTTAG5950.020.7666171
CGTACAC1856.5246822E-620.5514053
GTATCAA100250.020.1945691
AGAGTAC70200.019.83978710-11
GTATAGA4450.019.223031
AGTACTT49150.018.80819312-13
CATGGGG31850.018.206034
TCAACGC111400.018.004964
CAACGCA112200.017.7506315
TATCAAC113900.017.6920932
ATCAACG113450.017.594143
AACGCAG113700.017.5164536
ACTTTTT61850.017.09429416-17
GAGTACA38200.017.0438061
Produced by FastQC (version 0.11.5)