##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936700_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1566792 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.380155119505332 31.0 31.0 34.0 30.0 34.0 2 31.45089903446022 31.0 31.0 34.0 28.0 34.0 3 31.407219975593442 33.0 31.0 34.0 28.0 34.0 4 35.05743583066546 37.0 35.0 37.0 32.0 37.0 5 35.084160501202454 37.0 35.0 37.0 32.0 37.0 6 35.13159308957411 37.0 35.0 37.0 32.0 37.0 7 34.9954346205495 37.0 35.0 37.0 32.0 37.0 8 34.9817021021297 37.0 35.0 37.0 32.0 37.0 9 36.48568029451261 39.0 37.0 39.0 32.0 39.0 10-11 36.367471240598626 39.0 37.0 39.0 32.0 39.0 12-13 36.27597856001307 39.0 36.0 39.0 32.0 39.0 14-15 37.29012561973765 40.0 37.0 41.0 32.0 41.0 16-17 37.19101067659268 40.0 37.0 41.0 31.0 41.0 18-19 37.05449032162534 39.5 36.0 41.0 31.0 41.0 20-21 37.03125015956171 39.0 36.0 41.0 31.0 41.0 22-23 37.01851171055252 39.0 36.0 41.0 31.0 41.0 24-25 37.136925960816754 39.0 37.0 41.0 31.0 41.0 26-27 37.061705063594914 39.0 36.0 41.0 31.0 41.0 28-29 36.95328033331802 39.0 36.0 41.0 30.5 41.0 30-31 36.76742828658814 39.0 36.0 41.0 30.0 41.0 32-33 36.594271607207595 39.0 36.0 41.0 30.0 41.0 34-35 36.4076278153067 39.0 35.0 41.0 30.0 41.0 36-37 36.202499757466214 39.0 35.0 40.0 29.5 41.0 38-39 36.00298635683613 39.0 35.0 40.0 29.0 41.0 40-41 35.77821561509122 38.5 35.0 40.0 28.0 41.0 42-43 35.51240719891345 38.0 35.0 40.0 27.0 41.0 44-45 35.37611820841567 38.0 34.5 40.0 27.0 41.0 46-47 35.27406413869869 38.0 34.5 40.0 26.0 41.0 48-49 35.05958480768347 38.0 34.0 40.0 25.5 41.0 50-51 34.07559586722424 37.5 33.0 39.5 24.0 40.5 52-53 34.18678803568055 37.5 33.5 39.5 24.0 40.5 54-55 34.63090186827607 38.0 34.0 40.0 24.5 41.0 56-57 34.45090509780494 38.0 34.0 40.0 24.0 41.0 58-59 34.21525097141165 38.0 33.5 40.0 23.0 41.0 60-61 33.90690819202549 37.0 33.0 40.0 23.0 41.0 62-63 33.57249239209799 37.0 33.0 40.0 22.0 41.0 64-65 33.209785344831985 36.0 32.5 39.0 22.0 41.0 66-67 32.82272407569097 36.0 32.0 39.0 20.5 41.0 68-69 32.37489149804186 35.0 31.5 39.0 19.5 40.0 70-71 31.961170978662132 35.0 31.0 38.0 18.0 40.0 72-73 31.547932016502507 35.0 31.0 37.0 17.0 39.5 74-75 31.138410203779443 35.0 31.0 37.0 13.5 39.0 76-77 30.68229701198372 34.5 30.0 36.0 9.5 39.0 78-79 30.216232914132824 34.0 30.0 36.0 7.0 38.5 80-81 29.777050176411418 34.0 29.0 35.0 6.5 37.0 82-83 29.38812586482443 34.0 29.0 35.0 2.0 37.0 84-85 29.017645928751232 34.0 29.0 35.0 2.0 36.0 86-87 28.690539012198172 34.0 29.0 35.0 2.0 36.0 88-89 28.323537840377025 34.0 28.5 35.0 2.0 35.5 90-91 27.96545744425552 34.0 27.0 35.0 2.0 35.0 92-93 27.536245717363887 33.5 26.5 35.0 2.0 35.0 94-95 27.050103013035553 33.0 25.5 35.0 2.0 35.0 96-97 26.46323411148385 33.0 25.0 35.0 2.0 35.0 98-99 25.71825902863941 33.0 23.5 35.0 2.0 35.0 100-101 23.16433387456663 29.5 9.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.42845958668932127 1101 2 0.3279721591552338 1101 3 0.4108956419416643 1101 4 0.5119058553407925 1101 5 0.4371393639104113 1101 6 0.4398971665596676 1101 7 0.36793992145240395 1101 8 0.3552889666416519 1101 9 0.4889595088087475 1101 10-11 0.6119090206053883 1101 12-13 0.6579409627711215 1101 14-15 0.7925130326852496 1101 16-17 0.804020923934722 1101 18-19 0.8891365165064258 1101 20-21 0.7782769793301227 1101 22-23 0.6333021949689055 1101 24-25 0.5771970981712897 1101 26-27 0.6019839025558298 1101 28-29 0.6745778132676179 1101 30-31 0.45844632712586275 1101 32-33 0.4049446870390909 1101 34-35 0.4470737976961061 1101 36-37 0.5991791157270967 1101 38-39 0.7253328919569739 1101 40-41 0.6014853373556122 1101 42-43 0.7068999954785085 1101 44-45 0.5410985178491785 1101 46-47 0.757002432735078 1101 48-49 0.751437106541367 1101 50-51 0.7668343599211553 1101 52-53 0.6781084063747755 1101 54-55 0.75328135237541 1101 56-57 0.8434920132903656 1101 58-59 0.8442796265116925 1101 60-61 0.7161119109617857 1101 62-63 0.6755892366326108 1101 64-65 0.5607017400133785 1101 66-67 0.5627273516101639 1101 68-69 0.6656381560925979 1101 70-71 0.48954647912200855 1101 72-73 0.7029582210869734 1101 74-75 0.596114838450795 1101 76-77 0.4709679214472722 1101 78-79 0.3435363177763229 1101 80-81 0.4542457966188458 1101 82-83 0.6773489731087352 1101 84-85 0.6436775187318702 1101 86-87 0.6382291591428775 1101 88-89 0.7475243149664017 1101 90-91 0.5248904871292872 1101 92-93 0.5721641340635593 1101 94-95 0.7285395879075232 1101 96-97 0.6474618468934565 1101 98-99 0.5960540915836248 1101 100-101 0.9701254142487628 1102 1 0.23668056153582384 1102 2 0.36348708032785737 1102 3 0.46121812930294936 1102 4 0.33128040026955574 1102 5 0.3774665504470178 1102 6 0.2338910008285353 1102 7 0.3450926343224694 1102 8 0.2647399893577784 1102 9 0.38747849399343437 1102 10-11 0.47733496728266545 1102 12-13 0.38290339518820105 1102 14-15 0.5174824364063753 1102 16-17 0.49877979427363783 1102 18-19 0.510552104342274 1102 20-21 0.5027181131223557 1102 22-23 0.19546298938773532 1102 24-25 0.3773063152680223 1102 26-27 0.19905776223122018 1102 28-29 0.09383100586667581 1102 30-31 0.0711635348862174 1102 32-33 0.13013020118567198 1102 34-35 0.0013004735201391782 1102 36-37 7.078968849540956E-4 1102 38-39 -0.0782712722353125 1102 40-41 0.034663838886721976 1102 42-43 -0.08936390194521238 1102 44-45 0.08802778751470441 1102 46-47 0.09055557730959407 1102 48-49 0.22385294740973194 1102 50-51 0.18032254226980626 1102 52-53 0.045630649998607 1102 54-55 0.3123344943679669 1102 56-57 0.12313012515139832 1102 58-59 -0.08640056620613024 1102 60-61 0.13933306654579525 1102 62-63 0.07537209342136464 1102 64-65 0.12025655770086985 1102 66-67 -0.020875366682020058 1102 68-69 0.07749556968715154 1102 70-71 -0.193647772392314 1102 72-73 0.1991188399322148 1102 74-75 0.042687538824147 1102 76-77 0.1915423052614571 1102 78-79 0.12802155152156303 1102 80-81 0.22964277365485586 1102 82-83 0.025705552728773284 1102 84-85 0.1626141624879054 1102 86-87 -0.051568982875494385 1102 88-89 -0.02008700643137118 1102 90-91 0.24289865646484543 1102 92-93 0.27794127954692627 1102 94-95 0.1561619601131916 1102 96-97 0.08289827634644098 1102 98-99 0.1717732977844726 1102 100-101 0.8124804756736985 1103 1 0.3355777223607568 1103 2 0.32899997305798934 1103 3 0.39898401296150965 1103 4 0.29706495673777766 1103 5 0.3427734621610341 1103 6 0.2580433258917978 1103 7 0.2900468350740866 1103 8 0.19792121902240467 1103 9 0.43041134838587425 1103 10-11 0.2065025097189519 1103 12-13 0.2730479917596682 1103 14-15 0.49949325251156296 1103 16-17 0.6631973093859145 1103 18-19 0.6924556736990226 1103 20-21 0.836844017967536 1103 22-23 0.3777593414218998 1103 24-25 0.35889912653055944 1103 26-27 0.40025418614856534 1103 28-29 0.3370985268641249 1103 30-31 0.24776309307795685 1103 32-33 0.34842357490249753 1103 34-35 0.5655820516710222 1103 36-37 0.628206730359814 1103 38-39 0.33882504701004024 1103 40-41 0.5687353058919271 1103 42-43 0.7508741742761771 1103 44-45 0.6783583450086326 1103 46-47 0.8541132793181134 1103 48-49 0.8968612370947042 1103 50-51 0.8245828182002981 1103 52-53 0.8275992189770349 1103 54-55 0.7271124762584478 1103 56-57 0.9085914385536071 1103 58-59 0.7245240993899955 1103 60-61 0.6757961728235955 1103 62-63 0.7368089266103794 1103 64-65 0.7066059540503815 1103 66-67 0.7779468632936855 1103 68-69 0.9983082173419149 1103 70-71 0.9331884537832487 1103 72-73 0.7875863727000052 1103 74-75 0.7771202082540398 1103 76-77 0.7208483594218507 1103 78-79 0.6262236840556348 1103 80-81 0.7528815834208764 1103 82-83 0.5918049658930613 1103 84-85 0.690655955615707 1103 86-87 0.6249457132394518 1103 88-89 0.588664401806188 1103 90-91 0.8573463330830542 1103 92-93 0.6648768810535337 1103 94-95 0.7409349711352782 1103 96-97 0.9104154484372735 1103 98-99 1.1894650703141707 1103 100-101 1.4539040462074837 1104 1 0.3060229866421622 1104 2 0.4273909551326689 1104 3 0.5033247016245568 1104 4 0.4875048737978389 1104 5 0.4813603613632722 1104 6 0.4278898490377827 1104 7 0.35312427169844085 1104 8 0.5251189586348701 1104 9 0.6362759423978304 1104 10-11 0.6647040082065487 1104 12-13 0.6308911367695558 1104 14-15 0.7996197612295433 1104 16-17 0.6760672906970129 1104 18-19 0.7854144009548776 1104 20-21 0.7915827423968409 1104 22-23 0.6833646426592495 1104 24-25 0.6585730462231041 1104 26-27 0.520541749587494 1104 28-29 0.4064269126993665 1104 30-31 0.43478914680751046 1104 32-33 0.5255243104354648 1104 34-35 0.6323987200251722 1104 36-37 0.6063206957832179 1104 38-39 0.6003656057366626 1104 40-41 0.7582513243530187 1104 42-43 0.8278796986835459 1104 44-45 1.0405743200169653 1104 46-47 0.9366119662430492 1104 48-49 0.8398068072329536 1104 50-51 0.8258051908900228 1104 52-53 0.5366505248802795 1104 54-55 0.7025007562461312 1104 56-57 0.7720848932171904 1104 58-59 0.6852201768287287 1104 60-61 0.750897606553913 1104 62-63 0.7183666818742722 1104 64-65 0.6220126750962862 1104 66-67 0.6342571645521247 1104 68-69 0.6402206505280077 1104 70-71 0.6389519304685187 1104 72-73 0.720182511942923 1104 74-75 0.6641446008645389 1104 76-77 0.6419278020452168 1104 78-79 0.35448293147905474 1104 80-81 0.5875708381426712 1104 82-83 0.5888547657150305 1104 84-85 0.5600281897206436 1104 86-87 0.5911930923627189 1104 88-89 0.5898721408280387 1104 90-91 0.536585825318511 1104 92-93 0.5681616517030896 1104 94-95 0.8349409944057129 1104 96-97 0.8119269085767442 1104 98-99 0.6506104409071973 1104 100-101 1.2241798499781709 1105 1 0.4147953234098871 1105 2 0.3307861359373092 1105 3 0.43679547378301464 1105 4 0.5715169198138099 1105 5 0.5160695819936123 1105 6 0.34069114736918493 1105 7 0.47296114396317535 1105 8 0.5578186690034599 1105 9 0.524657785640251 1105 10-11 0.6584565378462273 1105 12-13 0.6055205766077236 1105 14-15 0.9146028811855516 1105 16-17 0.8610124532369596 1105 18-19 0.7743617000294947 1105 20-21 0.6775282418276021 1105 22-23 0.5364494815473364 1105 24-25 0.3913298781240542 1105 26-27 0.5004987190850656 1105 28-29 0.5634626049784117 1105 30-31 0.44884546201107156 1105 32-33 0.4878564468991229 1105 34-35 0.3824395492972812 1105 36-37 0.4652612807108696 1105 38-39 0.3154684126603584 1105 40-41 0.2999318966413469 1105 42-43 0.25942577501022157 1105 44-45 0.30032383736369184 1105 46-47 0.4629786028499083 1105 48-49 0.6334974815116112 1105 50-51 0.507253632000797 1105 52-53 0.6639936714404016 1105 54-55 0.674179837378432 1105 56-57 0.6632095956763351 1105 58-59 0.5830353571126494 1105 60-61 0.41440286465408604 1105 62-63 0.6315036875540798 1105 64-65 0.6543336791729004 1105 66-67 0.6960316560008621 1105 68-69 0.6460140678236854 1105 70-71 0.833552525510779 1105 72-73 0.6701274022877257 1105 74-75 0.6632704205072457 1105 76-77 0.72835173375816 1105 78-79 0.9105302792332104 1105 80-81 0.9241689353013491 1105 82-83 0.6219911717765427 1105 84-85 0.8189291682819224 1105 86-87 1.017009263594769 1105 88-89 0.8944084110286674 1105 90-91 0.9121287362917663 1105 92-93 0.9670222191471289 1105 94-95 0.9722879043596109 1105 96-97 1.0609049933170098 1105 98-99 1.3429699625695868 1105 100-101 1.4933133936512917 1106 1 0.06355811818726664 1106 2 0.023829473319576522 1106 3 -0.00402737991735691 1106 4 0.1705573728277514 1106 5 0.33696536953780054 1106 6 0.011407808692958099 1106 7 0.2172991989316273 1106 8 0.3015678122177263 1106 9 0.41667242331899246 1106 10-11 0.5494463471008686 1106 12-13 0.4436403757360523 1106 14-15 0.3507124521815044 1106 16-17 0.13105262173837673 1106 18-19 0.4177626402200829 1106 20-21 0.09277572662288947 1106 22-23 -0.07364334165743003 1106 24-25 -0.3245144996247902 1106 26-27 -0.28641359882227846 1106 28-29 -0.09835090912775968 1106 30-31 -0.09195835347951231 1106 32-33 -0.10350906206938504 1106 34-35 -0.0866420532957406 1106 36-37 -0.38558017899481456 1106 38-39 -0.21308916718543713 1106 40-41 -0.30092315231575384 1106 42-43 -0.23201922776463846 1106 44-45 -0.24504411051086095 1106 46-47 -0.3032564426760729 1106 48-49 -0.4406913462411097 1106 50-51 -0.47187128914839604 1106 52-53 -0.24104808667198085 1106 54-55 0.012630153616889572 1106 56-57 0.08651186066646943 1106 58-59 -0.08107626513108812 1106 60-61 -0.11926825106720429 1106 62-63 -0.12822270314339335 1106 64-65 -0.3474571852970527 1106 66-67 -0.32035287129073 1106 68-69 -0.24864192259675377 1106 70-71 -0.17409444456343337 1106 72-73 -0.010088874957396854 1106 74-75 0.15973708256019492 1106 76-77 -0.07689241802462732 1106 78-79 5.802160862735661E-4 1106 80-81 0.0905842339612768 1106 82-83 0.23427948769839801 1106 84-85 0.3778765567990163 1106 86-87 0.4162959405866147 1106 88-89 0.6382417413127044 1106 90-91 0.6905247697682562 1106 92-93 0.6377280090697646 1106 94-95 0.8146243487697618 1106 96-97 0.7241263538702114 1106 98-99 0.9402804044443194 1106 100-101 1.3370697491121675 1107 1 0.16085816171574407 1107 2 0.2804860891853771 1107 3 0.2542568570839663 1107 4 0.2753067400771343 1107 5 0.3998747390099098 1107 6 0.3280458753404787 1107 7 0.42694452782303216 1107 8 0.4679519186336165 1107 9 0.425618650431808 1107 10-11 0.5383467171590937 1107 12-13 0.3715532446862184 1107 14-15 0.1908094585070259 1107 16-17 0.35426494413490417 1107 18-19 0.6708943753922867 1107 20-21 0.3963723168479021 1107 22-23 0.4211174964708704 1107 24-25 0.3204963295728973 1107 26-27 0.23549035538946583 1107 28-29 0.3064922626562563 1107 30-31 0.2094811293651091 1107 32-33 0.4287066433764366 1107 34-35 0.3676060749134962 1107 36-37 0.4254824054325752 1107 38-39 0.5360469446496765 1107 40-41 0.5452158451003228 1107 42-43 0.4341017231937201 1107 44-45 0.5101953821243583 1107 46-47 0.6927526969815432 1107 48-49 0.7852723994308448 1107 50-51 0.6756960774579852 1107 52-53 0.7670824775973486 1107 54-55 0.8776337854003486 1107 56-57 0.9241106762684552 1107 58-59 1.0764863781722056 1107 60-61 0.9705231959173517 1107 62-63 0.9814796493790752 1107 64-65 0.961552548411845 1107 66-67 0.7697230323221262 1107 68-69 0.832063847374414 1107 70-71 0.7133899930128926 1107 72-73 0.7907852668766466 1107 74-75 0.8968279776988979 1107 76-77 0.9434269143093559 1107 78-79 0.9598537006270504 1107 80-81 0.92092464254684 1107 82-83 0.8278278790630864 1107 84-85 0.8544405059566955 1107 86-87 0.9646329277543124 1107 88-89 0.950211147010041 1107 90-91 0.7691194746411973 1107 92-93 1.1132241212753335 1107 94-95 1.0482876076901206 1107 96-97 1.1515364622680337 1107 98-99 1.2780076885979632 1107 100-101 1.7911731352044882 1108 1 0.12197207125510445 1108 2 0.05256858299023648 1108 3 0.3198480588255528 1108 4 0.21048819402465568 1108 5 0.3378361901783009 1108 6 0.24091410989894513 1108 7 0.4036878521241931 1108 8 0.43837706743279625 1108 9 0.03241883107517651 1108 10-11 0.3347296521506422 1108 12-13 0.06807209644286871 1108 14-15 0.49144750014763616 1108 16-17 0.6217028190277887 1108 18-19 0.6145575276190627 1108 20-21 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86-87 -4.470055529672347 2316 88-89 -6.132147660923028 2316 90-91 -5.197833546876883 2316 92-93 -4.573078808240133 2316 94-95 -4.172355043419401 2316 96-97 -5.8355414855695145 2316 98-99 -7.415925294057136 2316 100-101 -7.5787735010411765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4395.0 3 1707.0 4 1639.0 5 1744.0 6 2029.0 7 2214.0 8 2859.0 9 3496.0 10 4406.0 11 5365.0 12 6548.0 13 7542.0 14 8546.0 15 10154.0 16 11777.0 17 13980.0 18 11837.0 19 9938.0 20 10304.0 21 11111.0 22 12410.0 23 13789.0 24 15982.0 25 18188.0 26 21366.0 27 25275.0 28 30028.0 29 36212.0 30 43651.0 31 53509.0 32 66854.0 33 85656.0 34 113983.0 35 156469.0 36 219797.0 37 283477.0 38 209290.0 39 29258.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.49765594062568 17.62489940344647 14.480283075508094 23.39716158041976 2 15.702376453042962 18.902199227767678 40.209516537620786 25.185907781568574 3 19.944719816924287 22.732156022890848 29.407589215895925 27.91553494428894 4 13.007896534334284 15.773917817313645 37.00167889129338 34.21650675705868 5 14.328778404466192 36.1411102280018 34.35693150370108 15.17317986383093 6 32.998327310800974 34.82979429753438 17.2954147886155 14.876463603049148 7 29.182991715583263 31.580745916874786 20.43766247868216 18.79859988885979 8 26.597358808654114 33.37115890571918 19.80395412398784 20.227528161638865 9 25.74612805364841 17.02826121666933 20.47236148810474 36.75324924157752 10-11 25.275073703691525 25.346981301789356 27.291040894413015 22.086904100106107 12-13 26.47007181079799 22.76936072619124 27.547521100447643 23.213046362563123 14-15 23.083202988933575 24.39941802382884 26.00633379596416 26.511045191273425 16-17 22.690533703515076 27.46386656853338 26.680801265727798 23.16479846222374 18-19 22.583584694868332 26.275573980752526 28.59582693554768 22.54501438883147 20-21 23.27908964571586 25.2478916522131 28.570274961363186 22.902743740707855 22-23 22.82535495908627 25.081051890444783 28.86861199714794 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23.262107430196604 25.825684662544184 27.60114643099793 23.311061476261276 50-51 22.788152076960756 26.16965768801788 28.014916013652268 23.027274221369087 52-53 23.253381193349593 26.11649348365753 27.396880823006764 23.23324449998612 54-55 22.825816741815032 25.80831694366091 27.734006386946046 23.63185992757802 56-57 23.118980296541203 25.982300715502994 27.42055108410511 23.478167903850693 58-59 23.454816603866075 25.735456266756618 27.37632453295284 23.433402596424465 60-61 23.019374934177563 25.846532394213366 28.11838783697099 23.015704834638083 62-63 22.776879063561417 26.02003708964672 27.989822207769038 23.21326163902283 64-65 22.570642157918673 26.269707921463503 27.69064088142138 23.469009039196447 66-67 22.669585166836708 26.059230150374667 27.413211691573803 23.857972991214822 68-69 22.76018577529079 25.844664513351525 27.791583944026144 23.603565767331546 70-71 23.004912085415143 25.789501566301666 27.458180894438673 23.747405453844525 72-73 22.924907746191828 25.78389004807955 27.769856552948944 23.52134565277968 74-75 22.703740964209203 26.459897813536514 27.3706582881597 23.465702934094583 76-77 22.902982392412795 25.98869912226998 27.376666770937934 23.731651714379286 78-79 22.878799195395036 26.043404237390384 27.481466444257613 23.596330122956967 80-81 23.201973965158786 26.05667202649316 27.18932102977608 23.552032978571972 82-83 23.033527497679856 26.081371584092565 27.501158475637546 23.383942442590033 84-85 22.943485319305857 26.25582062563887 27.193152381593688 23.607541673461586 86-87 22.90561948066205 26.43247483347843 27.205565101061808 23.45634058479771 88-89 22.821359331743004 26.276796898062088 26.901652292545936 24.000191477648976 90-91 22.84231267341508 26.010841973103787 27.201282449803376 23.945562903677757 92-93 23.1129510170264 25.96720595726102 27.488341186728675 23.431501838983905 94-95 22.770748409615205 25.871950884781203 27.509922750002875 23.84737795560072 96-97 22.512101779097968 25.805170851556085 27.921717519086613 23.761009850259335 98-99 22.60520976837955 26.18264832020592 27.179142285510732 24.032999625903802 100-101 22.505275327236266 25.83539446935682 27.395889090932883 24.26344111247403 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 60.0 1 57.0 2 77.5 3 104.5 4 88.0 5 86.0 6 162.5 7 319.0 8 555.0 9 749.5 10 707.5 11 631.5 12 716.5 13 809.5 14 899.5 15 1002.0 16 1116.5 17 1265.5 18 1412.5 19 1571.5 20 1772.0 21 2083.0 22 2547.0 23 3156.5 24 3888.0 25 4887.0 26 6353.0 27 8145.5 28 10043.5 29 12443.0 30 15301.5 31 18347.0 32 22205.0 33 24934.0 34 26626.0 35 28831.5 36 31779.0 37 34818.5 38 37082.5 39 38924.0 40 40943.0 41 43841.0 42 46293.5 43 48462.5 44 51227.5 45 54549.5 46 57579.5 47 62880.0 48 71261.0 49 70409.0 50 62445.5 51 58347.5 52 57281.0 53 57770.5 54 57557.0 55 54586.0 56 50271.0 57 45918.0 58 42038.5 59 37195.0 60 30888.0 61 26074.5 62 22290.0 63 17474.0 64 12606.0 65 9424.5 66 7281.5 67 5712.5 68 4312.0 69 3279.0 70 2466.5 71 1772.5 72 1338.0 73 1119.0 74 935.0 75 702.5 76 527.5 77 375.5 78 252.5 79 170.0 80 115.0 81 83.5 82 65.0 83 38.0 84 22.0 85 20.0 86 17.5 87 9.5 88 7.0 89 5.5 90 5.0 91 6.5 92 3.5 93 2.0 94 1.5 95 1.5 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0722495391858013 2 0.02706166485404572 3 0.014488202645915986 4 0.017998560115190784 5 0.02278541120965642 6 0.028848755929312887 7 0.07671726687396924 8 0.05310213480793877 9 0.06650531787244254 10-11 0.05702735270540058 12-13 0.03784803598690828 14-15 0.04777277392276703 16-17 0.057091177386660134 18-19 0.05236815097345404 20-21 0.017934735433931242 22-23 0.012956410295686985 24-25 0.021094057156278562 26-27 0.02326409631910298 28-29 0.006382468125954178 30-31 0.0011807566033015231 32-33 0.009797088573339665 34-35 0.010786371132862562 36-37 0.004052867259980903 38-39 0.009382228145152643 40-41 0.00462728939131678 42-43 0.0022657761847137336 44-45 4.148604281870216E-4 46-47 6.06334471965647E-4 48-49 0.0010531072407824395 50-51 0.0012445812845610648 52-53 3.191234062977089E-5 54-55 1.5956170314885448E-4 56-57 0.003957130238091591 58-59 0.003861393216202278 60-61 0.004914500456984718 62-63 0.003031672359828235 64-65 2.8721106566793805E-4 66-67 3.5103574692747986E-4 68-69 0.003861393216202278 70-71 0.002808285975419839 72-73 0.020774933749980854 74-75 0.015573222227328197 76-77 0.02329600865973275 78-79 0.019721826509198414 80-81 0.012701111570648816 82-83 0.004978325138244259 84-85 0.0036699191724236533 86-87 0.00178709107526717 88-89 0.0018828280971564827 90-91 0.006573942169732804 92-93 0.010594897089083937 94-95 0.01212668943931294 96-97 0.011711829011125918 98-99 0.022338638440839626 100-101 0.019243141399751848 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1566792.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.48327121117195 #Duplication Level Percentage of deduplicated Percentage of total 1 82.13622358792684 53.78548605469673 2 10.90739703425068 14.2850407640354 3 3.1035289726136126 6.096876882761611 4 1.3278176284631353 3.477993675345066 5 0.7106885127352767 2.3269104313054276 6 0.4140604091623664 1.6268418042592838 7 0.2655097319535622 1.2170512050704478 8 0.1970520000572742 1.0322887649963486 9 0.15662962652177384 0.92309582819069 >10 0.7214503304946885 8.184970641479412 >50 0.037351053190422256 1.6816274633037092 >100 0.018185136953721866 2.2619701058397395 >500 0.0028350784433749062 1.2734324101735095 >1k 0.001270897233237027 1.8264139685426415 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4077 0.26021322549515186 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3720 0.23742781428549545 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3692 0.23564072321022828 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2897 0.18490010160889256 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2615 0.16690154149370176 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2347 0.14979652691614456 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1581 0.10090682107133557 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1575 0.10052387298377832 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.382468125954178E-5 2 0.0 0.0 0.0 0.0 6.382468125954178E-5 3 0.0 0.0 0.0 0.0 6.382468125954178E-5 4 0.0 0.0 0.0 0.0 6.382468125954178E-5 5 0.0 0.0 0.0 0.0 6.382468125954178E-5 6 0.0 0.0 0.0 0.0 6.382468125954178E-5 7 0.0 0.0 0.0 0.0 6.382468125954178E-5 8 0.0 0.0 0.0 0.0 6.382468125954178E-5 9 0.0 0.0 0.0 0.0 6.382468125954178E-5 10-11 0.0 0.0 0.0 0.0 6.382468125954178E-5 12-13 0.0 0.0 0.0 0.0 6.382468125954178E-5 14-15 0.0 0.0 0.0 0.0 6.382468125954178E-5 16-17 0.0 0.0 0.0 3.191234062977089E-5 6.382468125954178E-5 18-19 0.0 0.0 0.0 6.382468125954178E-5 6.382468125954178E-5 20-21 0.0 0.0 0.0 6.382468125954178E-5 6.382468125954178E-5 22-23 0.0 0.0 0.0 6.382468125954178E-5 6.382468125954178E-5 24-25 0.0 0.0 0.0 1.9147404377862538E-4 6.382468125954178E-5 26-27 0.0 0.0 0.0 3.5103574692747986E-4 6.382468125954178E-5 28-29 0.0 0.0 0.0 8.297208563740432E-4 6.382468125954178E-5 30-31 0.0 0.0 0.0 0.0020743021409351083 6.382468125954178E-5 32-33 0.0 0.0 0.0 0.0036060944911641113 1.2764936251908357E-4 34-35 0.0 0.0 0.0 0.004818763435095405 1.2764936251908357E-4 36-37 0.0 0.0 0.0 0.00606334471965647 1.2764936251908357E-4 38-39 0.0 0.0 0.0 0.007946172816812952 1.2764936251908357E-4 40-41 0.0 0.0 0.0 0.010818283473492334 1.2764936251908357E-4 42-43 0.0 0.0 0.0 0.015637046908587738 1.2764936251908357E-4 44-45 0.0 0.0 0.0 0.0239023431316984 1.9147404377862535E-4 46-47 0.0 0.0 0.0 0.03315692191433196 2.5529872503816714E-4 48-49 0.0 0.0 0.0 0.051251219051412056 2.5529872503816714E-4 50-51 0.0 0.0 0.0 0.07030288640738527 2.5529872503816714E-4 52-53 0.0 0.0 0.0 0.08769511205061042 2.5529872503816714E-4 54-55 0.0 0.0 0.0 0.11596944584858743 2.5529872503816714E-4 56-57 0.0 0.0 0.0 0.14784987413772854 2.5529872503816714E-4 58-59 0.0 0.0 0.0 0.18563408544337728 2.5529872503816714E-4 60-61 0.0 0.0 0.0 0.22852427124978938 2.5529872503816714E-4 62-63 0.0 0.0 0.0 0.28686002992101056 2.5529872503816714E-4 64-65 0.0 0.0 0.0 0.33747300215982723 2.5529872503816714E-4 66-67 0.0 0.0 0.0 0.4110309473114491 2.5529872503816714E-4 68-69 0.0 0.0 0.0 0.5094805181542923 2.5529872503816714E-4 70-71 0.0 0.0 0.0 0.5725073908980899 2.5529872503816714E-4 72-73 0.0 0.0 0.0 0.6612875225301125 2.8721106566793805E-4 74-75 0.0 0.0 0.0 0.7693746202431465 3.1912340629770895E-4 76-77 0.0 6.382468125954178E-5 0.0 0.853718936527631 3.1912340629770895E-4 78-79 0.0 6.382468125954178E-5 0.0 0.9394673957998254 3.1912340629770895E-4 80-81 0.0 6.382468125954178E-5 0.0 1.0646914204310463 3.1912340629770895E-4 82-83 0.0 6.382468125954178E-5 0.0 1.2224341201640039 3.1912340629770895E-4 84-85 0.0 6.382468125954178E-5 0.0 1.426641187853908 3.1912340629770895E-4 86-87 0.0 6.382468125954178E-5 0.0 1.7027467589826857 3.8294808755725076E-4 88-89 0.0 1.2764936251908357E-4 0.0 2.0221254640054327 3.8294808755725076E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4680 0.0 24.675718 1 TACATGG 4850 0.0 23.224358 2 ACATGGG 4890 0.0 22.061764 3 GTGTAGC 915 0.0 21.814116 1 GTACTTT 4940 0.0 21.019049 14-15 GAGTACT 4920 0.0 20.769417 12-13 GTCTTAG 595 0.0 20.766617 1 CGTACAC 185 6.5246822E-6 20.551405 3 GTATCAA 10025 0.0 20.194569 1 AGAGTAC 7020 0.0 19.839787 10-11 GTATAGA 445 0.0 19.22303 1 AGTACTT 4915 0.0 18.808193 12-13 CATGGGG 3185 0.0 18.20603 4 TCAACGC 11140 0.0 18.00496 4 CAACGCA 11220 0.0 17.750631 5 TATCAAC 11390 0.0 17.692093 2 ATCAACG 11345 0.0 17.59414 3 AACGCAG 11370 0.0 17.516453 6 ACTTTTT 6185 0.0 17.094294 16-17 GAGTACA 3820 0.0 17.043806 1 >>END_MODULE