FastQCFastQC Report
Thu 26 May 2016
SRR936700_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936700_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566792
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT71860.45864415953106735No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37500.2393425547232817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34610.22089722183927413No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22510.14366935751522858No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21450.13690394130171712No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC18520.1182033096926714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18010.11494825094843476No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA17550.11201231561049584No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC16970.10831048409744241No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC16440.1049277759906867No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15740.10046004830251878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT1751.5279511E-1029.8518476
TAAGGTG9800.026.6534335
GTAAGGT10350.023.4016464
GTACATG50100.023.0443971
TAGACTG6450.022.0891045
TACATGG52100.021.5166192
GAGTACT50800.021.1755612-13
ACATGGG50850.021.1087133
GGTAAGG11400.020.4143493
GTACTTT52750.020.30273414-15
AGGTAAG11100.019.6849652
AGAGTAC78950.019.67035510-11
AGTACTT52350.019.36919812-13
ACTATAC4001.8189894E-1218.9978433
TATACAG8400.018.6574045
AAGGTAA10500.018.5520271
CTAGACT6550.018.1265544
CCTATAC4201.8189894E-1218.0931843
GGTGTGC15350.017.9446988
GTATAGG5050.017.8755321
Produced by FastQC (version 0.11.5)