##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936700_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1566792 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9695658389882 31.0 31.0 34.0 30.0 34.0 2 32.14123316943155 33.0 31.0 34.0 30.0 34.0 3 32.225353461084815 34.0 31.0 34.0 30.0 34.0 4 35.898121767279896 37.0 35.0 37.0 35.0 37.0 5 35.739345745957344 37.0 35.0 37.0 33.0 37.0 6 35.82829373650108 37.0 35.0 37.0 35.0 37.0 7 35.77561412108308 37.0 35.0 37.0 33.0 37.0 8 35.74117240833499 37.0 35.0 37.0 33.0 37.0 9 37.389593513369995 39.0 37.0 39.0 34.0 39.0 10-11 37.2804900076079 39.0 37.0 39.0 34.0 39.0 12-13 37.192300573400935 39.0 37.0 39.0 33.0 39.0 14-15 38.354440474549264 40.0 38.0 41.0 33.0 41.0 16-17 38.25401489157463 40.0 38.0 41.0 33.0 41.0 18-19 38.373113342421966 40.0 38.0 41.0 33.5 41.0 20-21 38.378489614447865 40.0 38.0 41.0 34.0 41.0 22-23 38.293795538910075 40.0 38.0 41.0 34.0 41.0 24-25 38.157110835388494 40.0 37.5 41.0 33.0 41.0 26-27 37.96203101624211 40.0 37.0 41.0 33.0 41.0 28-29 37.89801837129625 40.0 37.0 41.0 33.0 41.0 30-31 37.89923614621469 40.0 37.0 41.0 33.0 41.0 32-33 37.77221513768261 40.0 37.0 41.0 33.0 41.0 34-35 37.61200497577215 40.0 37.0 41.0 33.0 41.0 36-37 37.42181125509959 40.0 36.5 41.0 32.0 41.0 38-39 37.24389995608862 39.0 36.0 41.0 31.5 41.0 40-41 37.06694602729654 39.0 36.0 41.0 31.0 41.0 42-43 36.81666456045218 39.0 36.0 41.0 31.0 41.0 44-45 36.580885337683625 39.0 35.0 40.5 30.0 41.0 46-47 36.26148046454156 39.0 35.0 40.0 30.0 41.0 48-49 35.91042716582673 39.0 35.0 40.0 29.0 41.0 50-51 35.623506183335124 38.0 35.0 40.0 28.0 41.0 52-53 35.26313894888409 38.0 34.0 40.0 27.0 41.0 54-55 35.0772052065622 38.0 34.0 40.0 27.0 41.0 56-57 34.74818514518839 38.0 34.0 40.0 26.0 41.0 58-59 34.367364334257516 37.0 33.5 40.0 26.0 41.0 60-61 33.92814681208482 37.0 33.0 39.5 25.0 41.0 62-63 33.59578138004279 36.0 32.5 39.0 24.5 40.5 64-65 33.162826335595284 36.0 32.0 39.0 23.0 40.0 66-67 32.73843018090468 35.0 31.5 38.5 22.5 40.0 68-69 32.36062795827397 35.0 31.0 38.0 22.0 40.0 70-71 31.97440406895108 35.0 31.0 37.5 21.5 39.5 72-73 31.5115838605252 34.5 31.0 37.0 20.0 39.0 74-75 31.023042305551726 34.0 30.0 36.0 18.5 39.0 76-77 29.55220795102349 32.5 28.5 35.0 17.5 37.0 78-79 30.32141184024427 34.0 30.0 35.0 18.0 37.0 80-81 30.09645536867689 34.0 30.0 35.0 13.5 37.0 82-83 29.808778382835754 34.0 29.5 35.0 12.0 36.5 84-85 29.448745908837928 34.0 29.0 35.0 7.5 36.0 86-87 29.09135450015063 34.0 29.0 35.0 6.0 36.0 88-89 28.630381058876992 33.0 29.0 35.0 2.0 35.0 90-91 28.155237261870113 33.0 27.5 35.0 2.0 35.0 92-93 27.535458759043955 33.0 26.5 35.0 2.0 35.0 94-95 27.00487078055032 32.5 25.5 34.0 2.0 35.0 96-97 26.29298528458149 32.0 25.0 34.0 2.0 35.0 98-99 25.4186445935389 32.0 22.0 34.0 2.0 35.0 100-101 22.17349590756144 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21448759014162633 1101 2 0.23702221928895995 1101 3 0.1953575477204481 1101 4 0.1991153891098918 1101 5 0.2784833118013452 1101 6 0.1828038654307349 1101 7 0.24068982914575088 1101 8 0.1925640897470302 1101 9 0.16541919902928015 1101 10-11 0.3214593314787848 1101 12-13 0.28598964259169435 1101 14-15 0.442748640622618 1101 16-17 0.36941579979051653 1101 18-19 0.27697805313156465 1101 20-21 0.24335194563914087 1101 22-23 0.1942221126421657 1101 24-25 0.16475804130325145 1101 26-27 0.2280028385589219 1101 28-29 0.23041291009433706 1101 30-31 0.24496860105126217 1101 32-33 0.12841236640075948 1101 34-35 0.19792974598988877 1101 36-37 0.22423285431720785 1101 38-39 0.16268618366126475 1101 40-41 0.2895821474133342 1101 42-43 0.25490750920716465 1101 44-45 0.22474171227458584 1101 46-47 0.1519431875982633 1101 48-49 0.0778541938352788 1101 50-51 0.05724031521700823 1101 52-53 0.26680095227253986 1101 54-55 0.1886528728042336 1101 56-57 0.34722917756505467 1101 58-59 0.026531195053394185 1101 60-61 0.2786984711256011 1101 62-63 0.09182916592089896 1101 64-65 0.10866849274389523 1101 66-67 -0.1374229616892535 1101 68-69 -0.22903371185038424 1101 70-71 -0.2224691576171729 1101 72-73 0.16585687891424072 1101 74-75 0.031048916366550827 1101 76-77 -0.15638673234742484 1101 78-79 -0.001274951848770911 1101 80-81 0.003070363340427207 1101 82-83 0.04017325165350982 1101 84-85 -0.022208944395725183 1101 86-87 -0.2399764651060785 1101 88-89 -0.06551929591968886 1101 90-91 -0.05953988828089862 1101 92-93 0.02385449395532646 1101 94-95 -0.10300030570802221 1101 96-97 -0.042316811529548914 1101 98-99 -0.342625609607186 1101 100-101 0.39472837193875066 1102 1 0.28142811717220795 1102 2 0.2128039090297804 1102 3 0.2587132752922159 1102 4 0.19210897034623997 1102 5 0.198574093671823 1102 6 0.17158574623692857 1102 7 0.1490992873979664 1102 8 0.24525991884164 1102 9 0.20819925180463628 1102 10-11 0.27557653200963017 1102 12-13 0.31231586863751915 1102 14-15 0.22634051466406646 1102 16-17 0.3627112126724583 1102 18-19 0.27568965286510405 1102 20-21 0.18352989462984937 1102 22-23 0.2734966839069699 1102 24-25 0.19944673384808453 1102 26-27 0.16090868464285535 1102 28-29 0.25272030845530224 1102 30-31 0.3385253087367417 1102 32-33 0.27997703686462927 1102 34-35 0.19821350388424008 1102 36-37 0.32440352216814006 1102 38-39 0.23514385235391444 1102 40-41 0.4064550269750171 1102 42-43 0.3129624637387636 1102 44-45 0.22959864562375998 1102 46-47 0.16770014211954276 1102 48-49 0.29849596692191227 1102 50-51 0.2818490185028182 1102 52-53 0.4059355294242195 1102 54-55 0.18838254112507968 1102 56-57 0.17952438905376766 1102 58-59 0.35406913702116327 1102 60-61 0.3069869901624216 1102 62-63 0.3556150487720018 1102 64-65 0.19107498690090807 1102 66-67 0.12086720980482113 1102 68-69 0.177093289817563 1102 70-71 0.27825301784280043 1102 72-73 0.2402063529680838 1102 74-75 0.1455528435344995 1102 76-77 0.1566945324020459 1102 78-79 0.21370328219081358 1102 80-81 0.2125371360397672 1102 82-83 0.2333586728493735 1102 84-85 0.32474382114641287 1102 86-87 0.3125654789066026 1102 88-89 0.28855013826861864 1102 90-91 0.29486391030522086 1102 92-93 0.3339786348066518 1102 94-95 0.018056389067631073 1102 96-97 0.3680957347521634 1102 98-99 0.4560116295498595 1102 100-101 1.0533611701169185 1103 1 0.18150367857557015 1103 2 0.18784237283176708 1103 3 0.1892819090075264 1103 4 0.16549748417219234 1103 5 0.22483509684140301 1103 6 0.17326564753418694 1103 7 0.08337085024739821 1103 8 0.08698557735034029 1103 9 0.2573083839099297 1103 10-11 0.18044824577168583 1103 12-13 0.26323011958124454 1103 14-15 0.26498717735123734 1103 16-17 0.26105301294653316 1103 18-19 0.19018546178198648 1103 20-21 0.0999189423249689 1103 22-23 0.025191823595719143 1103 24-25 0.13954251502057957 1103 26-27 0.01004808334644025 1103 28-29 0.1338369462566007 1103 30-31 0.1544087571111632 1103 32-33 0.1005441887777323 1103 34-35 0.10365902190547871 1103 36-37 0.052591341123431334 1103 38-39 0.2155478063486811 1103 40-41 0.2861279585472758 1103 42-43 0.22709570403034007 1103 44-45 0.29856103727354366 1103 46-47 0.3813153296463412 1103 48-49 0.2535487548119093 1103 50-51 0.3183126502423903 1103 52-53 0.2713608276763253 1103 54-55 0.36439279393524515 1103 56-57 0.1160748796248825 1103 58-59 0.276395114590251 1103 60-61 0.4278355794026467 1103 62-63 0.5226793148490287 1103 64-65 0.5741100856456924 1103 66-67 0.24741022830948367 1103 68-69 0.27240653860059183 1103 70-71 0.2730464798372054 1103 72-73 0.38626186905561966 1103 74-75 0.31435218439524704 1103 76-77 0.2438720565102308 1103 78-79 0.14244987719850855 1103 80-81 0.3417321601269343 1103 82-83 0.5128341884324676 1103 84-85 0.6684819982564534 1103 86-87 0.5552344989414095 1103 88-89 0.3757794203617202 1103 90-91 0.3889063297834596 1103 92-93 0.31297356304959223 1103 94-95 0.029865617017154733 1103 96-97 0.24172454224818907 1103 98-99 0.3547023364430757 1103 100-101 0.8404477367917131 1104 1 0.23677649233195908 1104 2 0.20987250289475412 1104 3 0.21898324507458256 1104 4 0.24486311598805344 1104 5 0.1716537110778802 1104 6 0.08471771871234779 1104 7 0.13288775463865932 1104 8 0.15984331384095896 1104 9 0.20053423493022393 1104 10-11 0.27057094182592323 1104 12-13 0.2537449979345965 1104 14-15 0.39086730678140924 1104 16-17 0.4566422672464583 1104 18-19 0.20872091375402135 1104 20-21 0.17626265580671685 1104 22-23 0.21213838091329507 1104 24-25 0.1605693819074574 1104 26-27 0.21862583848377426 1104 28-29 0.23908057177813902 1104 30-31 0.24863850411482957 1104 32-33 0.2116484005319137 1104 34-35 0.13688285321594407 1104 36-37 0.3981469511707303 1104 38-39 0.37784309320021237 1104 40-41 0.2934097244170317 1104 42-43 0.2512699960244831 1104 44-45 0.17593314165299034 1104 46-47 0.3671371511609465 1104 48-49 0.47137116483726516 1104 50-51 0.35948480440267616 1104 52-53 0.5440373439879878 1104 54-55 0.3952192085758739 1104 56-57 0.4142552423500021 1104 58-59 0.3128808036540818 1104 60-61 0.5490873829033305 1104 62-63 0.40507999574964515 1104 64-65 0.5552130106917801 1104 66-67 0.6233329403948318 1104 68-69 0.5699584446160628 1104 70-71 0.5460502102360039 1104 72-73 0.8316789470470098 1104 74-75 0.7517306326674706 1104 76-77 0.4141461200609591 1104 78-79 0.45847738216604483 1104 80-81 0.626529835825874 1104 82-83 0.5251563959594989 1104 84-85 0.6270637084391666 1104 86-87 0.6416304875761902 1104 88-89 0.4246677362084803 1104 90-91 0.5903882061835439 1104 92-93 0.7239878107050366 1104 94-95 0.8176827061881369 1104 96-97 1.2531887685991272 1104 98-99 1.2205863523645064 1104 100-101 1.7148652904432211 1105 1 -0.022304371718007587 1105 2 0.05930677092255365 1105 3 -0.04818142036602069 1105 4 0.10309993838978926 1105 5 0.11942301267247046 1105 6 0.12180568823912097 1105 7 -0.04085874725921457 1105 8 0.14212617114836945 1105 9 0.01975484160192309 1105 10-11 0.06805104593859568 1105 12-13 0.12047746481949417 1105 14-15 0.19960169402279604 1105 16-17 0.16684556151781038 1105 18-19 0.1409068459198366 1105 20-21 0.22548816199106625 1105 22-23 0.21376879039736707 1105 24-25 0.15738312074055472 1105 26-27 0.10785779845452481 1105 28-29 -0.017869674893780996 1105 30-31 0.0845541409765147 1105 32-33 0.09631655519871885 1105 34-35 0.228156197894684 1105 36-37 0.28562266071246967 1105 38-39 0.2337966322499625 1105 40-41 0.42699718545170384 1105 42-43 0.384730432657058 1105 44-45 0.3087722632753085 1105 46-47 0.26848968490328673 1105 48-49 0.45390649300599506 1105 50-51 0.5045407543124583 1105 52-53 0.3130553033449104 1105 54-55 0.19768008939937687 1105 56-57 0.14636353972596083 1105 58-59 0.1318568291788793 1105 60-61 0.37244417235762484 1105 62-63 0.3179353386247996 1105 64-65 0.39477409009938214 1105 66-67 0.28744255061927504 1105 68-69 0.3298180234788859 1105 70-71 0.46231627665319763 1105 72-73 0.449404137248294 1105 74-75 0.2648622733414747 1105 76-77 0.16123501268431184 1105 78-79 0.2850811835882432 1105 80-81 0.29738239047594917 1105 82-83 0.48939705434039027 1105 84-85 0.748613437703618 1105 86-87 0.4990274498783478 1105 88-89 0.31764849398399164 1105 90-91 0.2234943946388377 1105 92-93 0.30823339226772006 1105 94-95 0.5744116463706881 1105 96-97 0.6106019048158338 1105 98-99 0.3677689747951227 1105 100-101 0.7873469133259157 1106 1 0.07762925970384416 1106 2 0.13015701790222778 1106 3 0.11327426554912279 1106 4 0.10931678890246133 1106 5 0.1268182170057628 1106 6 -0.017077787045543857 1106 7 -0.03694902019236679 1106 8 0.05169944744131527 1106 9 0.09141646632487266 1106 10-11 -4.7205830779972757E-4 1106 12-13 0.09626354003951576 1106 14-15 0.1898052358192288 1106 16-17 0.1525613358943474 1106 18-19 0.11828133890818293 1106 20-21 -0.0159730815964636 1106 22-23 -0.009064970235066028 1106 24-25 -4.059862745933174E-4 1106 26-27 -0.18012787144784426 1106 28-29 -0.2282050364063153 1106 30-31 -0.06387376161466563 1106 32-33 0.07718434755920356 1106 34-35 0.07139377539650837 1106 36-37 -0.2727100959062909 1106 38-39 -0.019094829006888858 1106 40-41 0.0014125891134568747 1106 42-43 0.03767553499803711 1106 44-45 -0.010163622769077563 1106 46-47 0.04977008671026795 1106 48-49 -0.10509657829740604 1106 50-51 -0.027978523200737015 1106 52-53 -0.07068725869432768 1106 54-55 -0.09233654384436107 1106 56-57 -0.29895013068974663 1106 58-59 -0.24098436908286658 1106 60-61 -0.21659087942604316 1106 62-63 -0.2045833104683794 1106 64-65 -0.3023838074564864 1106 66-67 -0.17421415015442676 1106 68-69 -0.24318277225464158 1106 70-71 -0.11145082028231812 1106 72-73 -0.04025238746173443 1106 74-75 -0.0665968039205147 1106 76-77 -0.19897812511229063 1106 78-79 -0.27649695142554265 1106 80-81 -0.03830698760831197 1106 82-83 -0.0880860731124713 1106 84-85 0.30504873709007185 1106 86-87 0.3728685518884163 1106 88-89 0.10338365127327975 1106 90-91 0.10785105826416341 1106 92-93 0.048495467278801385 1106 94-95 0.21795073290253342 1106 96-97 0.488244019881396 1106 98-99 0.5829098933436647 1106 100-101 1.182498082810266 1107 1 0.033398503194746354 1107 2 0.13762248288296774 1107 3 0.14020767756007757 1107 4 0.0471403913935049 1107 5 0.02417250575172858 1107 6 1.0842090944862548E-4 1107 7 0.03790382311701279 1107 8 0.03395226455423028 1107 9 0.017943568426275647 1107 10-11 0.0370583553979813 1107 12-13 0.06310198322822913 1107 14-15 0.13169987573021302 1107 16-17 0.12699543258578672 1107 18-19 0.028607246820968157 1107 20-21 -0.04151358226659596 1107 22-23 0.012518407971853662 1107 24-25 -0.0231662210902428 1107 26-27 -0.11019480475400911 1107 28-29 0.0037944820020001657 1107 30-31 -0.08526119551157763 1107 32-33 -0.012602601041471928 1107 34-35 0.05197173834304891 1107 36-37 0.08244472126747837 1107 38-39 -0.07557931553620989 1107 40-41 0.14688317960573016 1107 42-43 -0.02331858138044396 1107 44-45 0.12117079635063988 1107 46-47 -0.07569764598517281 1107 48-49 -0.014878049454161157 1107 50-51 0.12671560654059277 1107 52-53 0.17155216911123716 1107 54-55 0.07839749979260802 1107 56-57 -0.008900688158583137 1107 58-59 0.016247113782924316 1107 60-61 0.033117585606298405 1107 62-63 0.17422969832860247 1107 64-65 0.14713625467413038 1107 66-67 0.22754643791665785 1107 68-69 0.3487480531855738 1107 70-71 0.19745306909977955 1107 72-73 0.21171506011555152 1107 74-75 0.36252295496611353 1107 76-77 0.15260351989377696 1107 78-79 0.4132083647380753 1107 80-81 0.48299219858111897 1107 82-83 0.4405027441697875 1107 84-85 0.5099540924160948 1107 86-87 0.6271747621719861 1107 88-89 0.47336408330336255 1107 90-91 0.6400033894193129 1107 92-93 0.657672170413246 1107 94-95 0.5509118470199184 1107 96-97 0.8085542231421954 1107 98-99 0.6816700593604956 1107 100-101 1.02876462264015 1108 1 0.04745278884286108 1108 2 0.1369433332963652 1108 3 0.05207600475616658 1108 4 0.1607882294629377 1108 5 0.07863879723601741 1108 6 0.07150944918708291 1108 7 -0.0010048477646620313 1108 8 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-1.2991378710968533 2316 50-51 -0.9776496426957095 2316 52-53 -1.1526083210912148 2316 54-55 -0.8860859072399307 2316 56-57 -0.9512378009575926 2316 58-59 -1.5779182847194875 2316 60-61 -1.1562769005126157 2316 62-63 -1.0218790731844294 2316 64-65 -0.8616473610171838 2316 66-67 -0.9164886328950956 2316 68-69 -1.4289467491713133 2316 70-71 -1.3037697475472037 2316 72-73 -0.8345164188964205 2316 74-75 -1.075660929224906 2316 76-77 -1.024930361943948 2316 78-79 -1.2122269387136484 2316 80-81 -0.8538370844709 2316 82-83 -1.0705528092690422 2316 84-85 -0.9096719354693263 2316 86-87 -0.8694858008705317 2316 88-89 -1.0636191724459607 2316 90-91 -1.0564393257894729 2316 92-93 -1.0153909001205896 2316 94-95 -1.0798069387697709 2316 96-97 -1.4623500919034242 2316 98-99 -1.9669873300962983 2316 100-101 -2.888897861138929 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 9.0 5 49.0 6 130.0 7 257.0 8 565.0 9 1053.0 10 1689.0 11 2592.0 12 3825.0 13 4963.0 14 6495.0 15 8684.0 16 10711.0 17 13407.0 18 12503.0 19 9599.0 20 9068.0 21 9278.0 22 10077.0 23 11115.0 24 13144.0 25 15988.0 26 19656.0 27 24444.0 28 30316.0 29 37816.0 30 46542.0 31 57887.0 32 72879.0 33 93357.0 34 124829.0 35 171289.0 36 245759.0 37 311290.0 38 176247.0 39 9276.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.22640200145935 19.03314294615173 14.806726261876324 22.933728790512603 2 16.228725869317376 20.457954747110573 38.5049796562435 24.80833972732855 3 20.462704096613177 23.352801501282986 29.18795399129358 26.996540410810262 4 13.74643220031759 16.69430275365205 36.1505547641295 33.40871028190085 5 14.40946525303161 37.1421951681867 33.473433069026726 14.974906509754963 6 33.02863430500028 35.960357213976074 16.41545272122911 14.595555759794534 7 29.650585399976514 32.08415667172158 19.797905529259786 18.46735239904212 8 27.039198566242362 34.24098412552527 18.801857553523377 19.917959754708985 9 26.157332945279272 17.32903920877819 19.650725814275283 36.862902031667254 10-11 25.622035343555496 26.047331107128453 26.556556326557708 21.77407722275835 12-13 26.742993035718786 23.284084784052393 26.95776466454618 23.01515751568264 14-15 23.174035032180488 25.251629448272517 25.389427286907996 26.184908232639003 16-17 22.888264683506172 28.17818191565951 26.004026060893853 22.929527339940464 18-19 22.819307260088138 27.052196593200634 28.07544269978175 22.05305344692948 20-21 23.517161180297066 25.821295998447784 28.20562652860112 22.455916292654035 22-23 23.08149145705551 25.765641853726407 28.226692792269542 22.926173896948537 24-25 23.398351536132427 25.979230172224522 28.150194792927202 22.47222349871585 26-27 22.968164501175345 26.0530402298227 28.3601708735088 22.618624395493157 28-29 22.722479972988825 26.974729361695065 27.87186797714262 22.430922688173485 30-31 23.01198067620517 26.104355895844517 27.897563973849433 22.98609945410088 32-33 22.917753767152252 26.21475201342978 27.99346383326392 22.87403038615405 34-35 23.54752133369927 26.10676742597829 27.80685742004251 22.53885382027994 36-37 23.577211124457648 25.828194349947854 28.03845716889778 22.55613735669672 38-39 23.210629998528823 26.339033689298418 27.854153775258517 22.596182536914238 40-41 23.37563864857016 26.28114259454871 27.651847573215214 22.691371183665915 42-43 23.162735270989288 26.48671989093026 27.20651124909364 23.144033588986815 44-45 23.365069659502005 26.255326264836317 27.37313979642116 23.006464279240525 46-47 24.011249763449246 26.670319574084182 26.83495570716788 22.48347495529869 48-49 23.87994257024024 26.721592357846507 26.586251386997628 22.812213684915626 50-51 23.253300297646685 27.077232086868026 27.028852932156532 22.640614683328756 52-53 23.84100465410764 27.00304699806228 26.405199682407577 22.750748665422506 54-55 23.281301310124498 26.9511051313236 26.705726054317847 23.06186750423406 56-57 23.658363761713865 26.89991190664658 26.603203278604802 22.838521053034754 58-59 23.925680841317646 26.60224949364846 26.43900399175792 23.03306567327597 60-61 23.36311642281177 26.955089799334413 27.12592335344479 22.555870424409026 62-63 23.11598014482836 26.986538520462876 27.15145568844539 22.74602564626337 64-65 23.05162865669736 27.371263505641647 26.65909127180453 22.918016565856462 66-67 23.29523296477588 26.898641062742424 26.575134722503236 23.23099124997846 68-69 23.34386025826283 26.833350778924363 26.629365014526645 23.193423948286163 70-71 23.596709323933226 26.901203447429634 26.41489082299133 23.08719640564581 72-73 23.50640672150483 26.742222324341714 26.71736261099112 23.034008343162334 74-75 23.325625375372024 27.609509701112923 26.13434951579004 22.93051540772502 76-77 23.641923440361687 26.863847844492362 26.271193742777715 23.223034972368232 78-79 23.573965615023457 27.143826914036286 26.27352681066241 23.00868066027785 80-81 23.853349219607527 27.202999709872287 26.102652865226585 22.8409982052936 82-83 23.662989890194634 27.23541476885718 26.289292237020263 22.81230310392793 84-85 23.359112431173447 27.668305819738283 26.07929355099039 22.893288198097885 86-87 23.440958435026783 27.550347233858393 25.883388532649303 23.125305798465526 88-89 23.253533034156707 27.522929921664087 25.671742622963382 23.55179442121583 90-91 23.51321450522434 27.296354536674144 25.861732612808215 23.328698345293304 92-93 23.660450069623078 27.31331534178515 26.080881522016025 22.945353066575752 94-95 23.293078845803926 27.251011150588816 26.136846103997673 23.319063899609585 96-97 23.185340507388467 27.289476062162798 26.500674842688376 23.024508587760362 98-99 23.11789587897517 27.54306657585398 25.70011824169522 23.638919303475628 100-101 22.85947677979622 27.549981574054737 25.83161049148415 23.758931154664893 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 156.0 1 103.5 2 68.0 3 105.0 4 111.0 5 100.5 6 161.5 7 297.5 8 473.0 9 627.0 10 655.5 11 634.5 12 692.5 13 836.5 14 975.0 15 1079.5 16 1231.0 17 1408.0 18 1564.5 19 1739.5 20 1983.5 21 2288.5 22 2680.5 23 3266.5 24 3927.0 25 4918.0 26 6409.0 27 8296.0 28 10461.0 29 12940.5 30 15689.0 31 18747.5 32 22382.0 33 24813.0 34 26527.5 35 28796.0 36 32046.0 37 35230.0 38 37377.0 39 39113.5 40 40845.0 41 43832.5 42 46168.0 43 48011.5 44 50835.5 45 54310.0 46 56991.5 47 61786.5 48 70785.0 49 69998.5 50 61383.5 51 57785.5 52 56755.0 53 57061.0 54 57681.0 55 55088.5 56 49737.5 57 45345.0 58 42750.5 59 38234.5 60 31470.0 61 26157.5 62 22230.0 63 17518.5 64 12736.0 65 9448.0 66 7368.5 67 5863.5 68 4359.5 69 3173.5 70 2427.0 71 1814.0 72 1355.5 73 1137.0 74 939.0 75 725.0 76 553.5 77 387.0 78 266.0 79 191.5 80 148.5 81 108.0 82 75.5 83 55.0 84 29.0 85 16.5 86 9.5 87 3.5 88 2.0 89 2.5 90 1.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021381268221946498 2 0.013913780514580112 3 0.008424857926259516 4 0.0 5 0.0032550587442366314 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.339838344847306E-4 14-15 2.5529872503816714E-4 16-17 0.0 18-19 8.616331970038142E-4 20-21 0.0 22-23 1.2764936251908357E-4 24-25 0.0 26-27 0.001212668943931294 28-29 0.002106214481564879 30-31 0.001212668943931294 32-33 0.007946172816812954 34-35 0.0014041429877099194 36-37 6.382468125954179E-4 38-39 0.0011807566033015231 40-41 4.467727688167925E-4 42-43 0.005616571950839678 44-45 0.00178709107526717 46-47 2.2338638440839626E-4 48-49 5.425097907061052E-4 50-51 9.573702188931269E-5 52-53 2.5529872503816714E-4 54-55 0.0020743021409351083 56-57 0.017360313302595368 58-59 0.02805094741356862 60-61 0.022657761847137338 62-63 0.015828520952366365 64-65 0.02023242395927475 66-67 0.003382708106755715 68-69 0.0010211949001526686 70-71 9.573702188931269E-5 72-73 0.0 74-75 8.297208563740432E-4 76-77 0.0016913540533778574 78-79 0.009477965167041955 80-81 0.0153498358429198 82-83 0.018700631609045743 84-85 0.034210029155114396 86-87 0.012541549867499963 88-89 0.04569847178183192 90-91 0.05134695607330138 92-93 0.03197616531103043 94-95 0.032359113398587686 96-97 0.05562320971769068 98-99 0.059452690593263184 100-101 0.067973285541412 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1566792.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.73108818166824 #Duplication Level Percentage of deduplicated Percentage of total 1 80.63073474015212 50.58053731137184 2 11.710693413615619 14.692490823960059 3 3.3336410849736113 6.2736879860253545 4 1.483676565533586 3.722905818622482 5 0.7822417201330041 2.4535437162521663 6 0.46193104373100846 1.7386462222883963 7 0.31335398145858473 1.375992537006872 8 0.22981796318170228 1.15333847312662 9 0.16629917514534828 0.9388915398523381 >10 0.8185439773902844 9.11528490539478 >50 0.04370835529639305 1.8808203725239958 >100 0.02097914329201973 2.528837067055498 >500 0.002240334747143316 0.931962804117439 >1k 0.002036667951948469 2.154010130374661 >5k 1.0183339759742346E-4 0.4590502920274122 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7186 0.45864415953106735 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3750 0.2393425547232817 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3461 0.22089722183927413 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2251 0.14366935751522858 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2145 0.13690394130171712 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1852 0.1182033096926714 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1801 0.11494825094843476 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1755 0.11201231561049584 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1697 0.10831048409744241 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1644 0.1049277759906867 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1574 0.10046004830251878 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 3.191234062977089E-5 3.191234062977089E-5 18-19 0.0 0.0 0.0 6.382468125954178E-5 6.382468125954178E-5 20-21 0.0 0.0 0.0 6.382468125954178E-5 6.382468125954178E-5 22-23 0.0 0.0 0.0 6.382468125954178E-5 6.382468125954178E-5 24-25 0.0 0.0 0.0 1.9147404377862538E-4 6.382468125954178E-5 26-27 0.0 0.0 0.0 3.5103574692747986E-4 6.382468125954178E-5 28-29 0.0 0.0 0.0 8.93545537633585E-4 6.382468125954178E-5 30-31 0.0 0.0 0.0 0.002106214481564879 9.573702188931268E-5 32-33 0.0 0.0 0.0 0.003669919172423653 1.2764936251908357E-4 34-35 0.0 0.0 0.0 0.004882588116354947 1.5956170314885448E-4 36-37 0.0 0.0 0.0 0.006127169400916012 1.9147404377862538E-4 38-39 0.0 0.0 0.0 0.008073822179332037 1.9147404377862538E-4 40-41 0.0 0.0 0.0 0.011009757517270959 1.9147404377862538E-4 42-43 0.0 0.0 0.0 0.015892345633625907 1.9147404377862538E-4 44-45 0.0 0.0 0.0 0.024093817175477025 1.9147404377862538E-4 46-47 0.0 0.0 0.0 0.03325265893622127 1.9147404377862538E-4 48-49 0.0 0.0 0.0 0.051251219051412056 1.9147404377862538E-4 50-51 0.0 0.0 0.0 0.07014332470423643 1.9147404377862538E-4 52-53 0.0 0.0 0.0 0.08763128736935089 2.5529872503816714E-4 54-55 0.0 0.0 0.0 0.11590562116732789 2.5529872503816714E-4 56-57 0.0 0.0 0.0 0.14784987413772854 2.5529872503816714E-4 58-59 0.0 0.0 0.0 0.18569791012463682 2.5529872503816714E-4 60-61 0.0 0.0 0.0 0.22874765763419777 2.5529872503816714E-4 62-63 0.0 0.0 0.0 0.2866366435366022 2.5529872503816714E-4 64-65 0.0 0.0 0.0 0.3368347553472318 2.5529872503816714E-4 66-67 0.0 0.0 0.0 0.4108394732676705 2.5529872503816714E-4 68-69 0.0 0.0 0.0 0.5092890441105137 2.5529872503816714E-4 70-71 0.0 0.0 0.0 0.5718372317448647 2.5529872503816714E-4 72-73 0.0 0.0 0.0 0.6600748535861811 2.5529872503816714E-4 74-75 0.0 0.0 0.0 0.7681619512992153 2.5529872503816714E-4 76-77 0.0 0.0 0.0 0.8528253909899974 2.5529872503816714E-4 78-79 0.0 0.0 0.0 0.9378717787683368 2.5529872503816714E-4 80-81 0.0 0.0 0.0 1.062521381268222 2.5529872503816714E-4 82-83 0.0 0.0 0.0 1.2193067107822864 2.5529872503816714E-4 84-85 0.0 0.0 0.0 1.4234499537909309 3.1912340629770895E-4 86-87 0.0 0.0 0.0 1.6970663623505864 3.1912340629770895E-4 88-89 0.0 0.0 0.0 2.0125836741571312 3.1912340629770895E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 175 1.5279511E-10 29.851847 6 TAAGGTG 980 0.0 26.653433 5 GTAAGGT 1035 0.0 23.401646 4 GTACATG 5010 0.0 23.044397 1 TAGACTG 645 0.0 22.089104 5 TACATGG 5210 0.0 21.516619 2 GAGTACT 5080 0.0 21.17556 12-13 ACATGGG 5085 0.0 21.108713 3 GGTAAGG 1140 0.0 20.414349 3 GTACTTT 5275 0.0 20.302734 14-15 AGGTAAG 1110 0.0 19.684965 2 AGAGTAC 7895 0.0 19.670355 10-11 AGTACTT 5235 0.0 19.369198 12-13 ACTATAC 400 1.8189894E-12 18.997843 3 TATACAG 840 0.0 18.657404 5 AAGGTAA 1050 0.0 18.552027 1 CTAGACT 655 0.0 18.126554 4 CCTATAC 420 1.8189894E-12 18.093184 3 GGTGTGC 1535 0.0 17.944698 8 GTATAGG 505 0.0 17.875532 1 >>END_MODULE