##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936699_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 268448 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.765056919775898 31.0 31.0 34.0 30.0 34.0 2 31.663588478960545 31.0 31.0 34.0 30.0 34.0 3 31.61587719036834 33.0 31.0 34.0 30.0 34.0 4 35.30686017403743 37.0 35.0 37.0 33.0 37.0 5 35.35989837882942 37.0 35.0 37.0 33.0 37.0 6 35.49389453450948 37.0 35.0 37.0 33.0 37.0 7 35.47411789247825 37.0 35.0 37.0 33.0 37.0 8 35.45110039933246 37.0 35.0 37.0 33.0 37.0 9 36.885609131004884 39.0 37.0 39.0 33.0 39.0 10-11 36.76686173858624 39.0 37.0 39.0 32.0 39.0 12-13 36.65866573787102 39.0 37.0 39.0 32.0 39.0 14-15 37.44822833472404 39.0 37.0 41.0 32.0 41.0 16-17 37.49211206639647 39.0 37.0 41.0 32.5 41.0 18-19 37.78512039575635 40.0 37.0 41.0 33.0 41.0 20-21 37.8285664262725 40.0 37.0 41.0 33.0 41.0 22-23 37.85994680534033 40.0 37.0 41.0 33.0 41.0 24-25 37.80014751460246 40.0 37.0 41.0 33.0 41.0 26-27 37.58328614852783 39.5 36.5 41.0 33.0 41.0 28-29 37.38719230539993 39.0 36.0 41.0 33.0 41.0 30-31 36.94221785969722 38.5 35.5 40.5 32.0 41.0 32-33 36.416823742400766 38.0 35.0 40.0 31.0 41.0 34-35 36.037891882226724 38.0 35.0 40.0 31.0 41.0 36-37 35.64593515317678 38.0 35.0 40.0 30.0 41.0 38-39 35.24406216473954 37.5 35.0 40.0 30.0 41.0 40-41 34.87425683931339 37.0 35.0 40.0 28.0 41.0 42-43 34.34367363511741 37.0 34.5 40.0 25.0 41.0 44-45 33.54786960603171 37.0 33.5 40.0 20.5 41.0 46-47 32.376678164858745 37.0 33.0 40.0 4.5 41.0 48-49 31.658218723924186 36.0 32.0 40.0 2.0 41.0 50-51 30.4866771218262 35.5 29.5 39.0 2.0 40.0 52-53 30.497945598402673 35.0 30.5 39.0 2.0 40.0 54-55 30.85284114614376 35.0 31.0 39.5 2.0 41.0 56-57 30.617840326618193 35.0 31.0 40.0 2.0 41.0 58-59 30.350678716175942 35.0 30.5 39.0 2.0 41.0 60-61 29.895950053641673 35.0 29.0 39.0 2.0 41.0 62-63 29.449884148885445 35.0 28.0 39.0 2.0 40.5 64-65 28.964762263082605 35.0 27.0 38.0 2.0 40.0 66-67 28.347650196686136 35.0 26.0 37.5 2.0 40.0 68-69 27.8928954583383 35.0 25.5 37.0 2.0 39.0 70-71 27.3421891763023 34.0 24.0 36.0 2.0 39.0 72-73 26.85525502145667 34.0 23.0 35.5 2.0 38.5 74-75 26.408628486708785 34.0 20.0 35.0 2.0 37.5 76-77 25.88342621289784 33.5 17.5 35.0 2.0 37.0 78-79 25.327564370008346 33.0 9.0 35.0 2.0 36.0 80-81 24.747137248182142 33.0 2.0 35.0 2.0 36.0 82-83 24.29675579628084 32.5 2.0 35.0 2.0 35.5 84-85 23.917116164024318 32.0 2.0 35.0 2.0 35.0 86-87 23.51976546668256 32.0 2.0 35.0 2.0 35.0 88-89 22.94783347240434 31.5 2.0 35.0 2.0 35.0 90-91 22.344753918822267 31.0 2.0 35.0 2.0 35.0 92-93 21.82746751698653 31.0 2.0 35.0 2.0 35.0 94-95 21.31351881928716 30.0 2.0 34.0 2.0 35.0 96-97 20.691821134819406 29.5 2.0 34.0 2.0 35.0 98-99 20.01749500834426 29.0 2.0 34.0 2.0 35.0 100-101 17.79090736380975 22.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18391987103340668 1101 2 0.47402147916819004 1101 3 0.5034526461668101 1101 4 0.48004593045991584 1101 5 0.32926893820432923 1101 6 0.46711715777117746 1101 7 0.5404262926749936 1101 8 0.46443465234421666 1101 9 0.42966053052073505 1101 10-11 0.5691817550124654 1101 12-13 0.599223633011313 1101 14-15 0.5842459201071861 1101 16-17 0.9786092835122417 1101 18-19 0.9013963234628832 1101 20-21 0.8845997173339697 1101 22-23 0.8049926309311246 1101 24-25 0.7933053613098693 1101 26-27 0.5167151381579345 1101 28-29 0.6893130740711584 1101 30-31 0.8403782964508508 1101 32-33 0.4833152461877148 1101 34-35 0.4925429109447279 1101 36-37 0.5798374216100441 1101 38-39 0.9167700758111081 1101 40-41 1.0654481120388866 1101 42-43 0.9122971535277316 1101 44-45 0.6049754361226789 1101 46-47 1.017708458884421 1101 48-49 0.8536734094294616 1101 50-51 0.9565610402571565 1101 52-53 1.282244544616038 1101 54-55 1.204914518256519 1101 56-57 1.1159248956366312 1101 58-59 0.8840177434627314 1101 60-61 0.844997744106486 1101 62-63 1.075002885467999 1101 64-65 1.178183090552622 1101 66-67 1.4786383978147981 1101 68-69 1.688385028139738 1101 70-71 1.690372720009016 1101 72-73 1.2357986685724462 1101 74-75 1.5834256177647532 1101 76-77 1.5699997036295876 1101 78-79 1.3049467763510378 1101 80-81 1.6733324232701463 1101 82-83 1.5863699275909653 1101 84-85 1.7276176569660286 1101 86-87 1.789533064189765 1101 88-89 1.8574193631500329 1101 90-91 1.7195720600595088 1101 92-93 1.9279873071935931 1101 94-95 2.2114649322255993 1101 96-97 1.6518032961432034 1101 98-99 2.0023690615926135 1101 100-101 2.405534566326434 1102 1 0.3207175005329681 1102 2 0.3618616898793441 1102 3 0.39162209305004225 1102 4 0.24080097874789175 1102 5 0.40010958789704176 1102 6 0.33525807085283077 1102 7 0.45965368512451477 1102 8 0.48775554786660535 1102 9 0.6087650081326785 1102 10-11 0.6155766189281806 1102 12-13 0.5398239402984117 1102 14-15 0.21917787796494537 1102 16-17 0.5279124002813305 1102 18-19 0.06936164391942867 1102 20-21 0.1482355821276471 1102 22-23 -0.03765991516194589 1102 24-25 -0.1090761136681806 1102 26-27 -0.2719701120615525 1102 28-29 -0.39637063971286324 1102 30-31 0.3325863737115995 1102 32-33 0.5580792935977215 1102 34-35 0.4460108652906456 1102 36-37 0.21800686849327633 1102 38-39 -0.11700406817484321 1102 40-41 0.12737194873774627 1102 42-43 0.19939109558216472 1102 44-45 0.6459653395467271 1102 46-47 0.6998858074357841 1102 48-49 0.8997116008254267 1102 50-51 0.7831084502659316 1102 52-53 0.9108112698135784 1102 54-55 0.8050846236120925 1102 56-57 0.7265208131080954 1102 58-59 0.9485205968428936 1102 60-61 0.6748375158360744 1102 62-63 0.9200972665039941 1102 64-65 0.6430371291829999 1102 66-67 0.9996820764802941 1102 68-69 1.10604854877187 1102 70-71 0.5505022195700349 1102 72-73 0.5511520487304828 1102 74-75 0.7789095949377121 1102 76-77 0.9240932067024552 1102 78-79 1.297950507694324 1102 80-81 1.411864029942663 1102 82-83 0.9985187423407424 1102 84-85 0.6835000099072062 1102 86-87 1.289258700713031 1102 88-89 1.3352233578822528 1102 90-91 1.4877897949146437 1102 92-93 1.3006003449460053 1102 94-95 1.2933898663783658 1102 96-97 1.0135515402169517 1102 98-99 1.5460202907409943 1102 100-101 1.4770062520156344 1103 1 0.3286853673950141 1103 2 0.29377502460825156 1103 3 0.19256934027633577 1103 4 0.47295505034094276 1103 5 0.47561461197073385 1103 6 0.6252544074657678 1103 7 0.5585651358257877 1103 8 0.7459850852331513 1103 9 0.5358112714778756 1103 10-11 0.8343527859786306 1103 12-13 0.46306943956573576 1103 14-15 0.6430710117309104 1103 16-17 0.6110254942733775 1103 18-19 0.6179669401304366 1103 20-21 0.6476926158308061 1103 22-23 0.6225020588803432 1103 24-25 0.7080554542614763 1103 26-27 0.47152737590160143 1103 28-29 0.7355963374694809 1103 30-31 0.7740506818547956 1103 32-33 0.4178042778974884 1103 34-35 0.6117267957909647 1103 36-37 0.25961247870889537 1103 38-39 0.06541298227522674 1103 40-41 -0.23129327001879574 1103 42-43 -0.1622898757387432 1103 44-45 -0.05388550826570082 1103 46-47 0.5045226087755381 1103 48-49 0.6119394635007396 1103 50-51 -0.004140346049741339 1103 52-53 0.05147224950794538 1103 54-55 0.3443286575245885 1103 56-57 0.22096685665006888 1103 58-59 0.07047867722807055 1103 60-61 0.053016812465759955 1103 62-63 0.0574350307912006 1103 64-65 0.13256376724035945 1103 66-67 0.5350533869261795 1103 68-69 0.5432410859290826 1103 70-71 0.4015986189838934 1103 72-73 0.8417502275037698 1103 74-75 0.7651261011131361 1103 76-77 0.7729130727271603 1103 78-79 0.622349973886493 1103 80-81 0.7382325666812051 1103 82-83 0.6307277647397385 1103 84-85 0.41222619789129666 1103 86-87 0.711267806846589 1103 88-89 0.6034290832328928 1103 90-91 0.34716806579594817 1103 92-93 0.31727529784531683 1103 94-95 0.34463803468483434 1103 96-97 0.32230311023998226 1103 98-99 0.1881793340736273 1103 100-101 0.22646328571879337 1104 1 0.1633617421376563 1104 2 0.39313281902065356 1104 3 0.37981794610390907 1104 4 0.638592279176109 1104 5 0.46252507133179677 1104 6 0.4049138067103897 1104 7 0.5900345731118932 1104 8 0.27056206127656424 1104 9 0.15857343725145512 1104 10-11 0.46158039400143025 1104 12-13 0.4460598542042007 1104 14-15 0.1935271004125525 1104 16-17 0.19568482534330656 1104 18-19 0.40876608385631386 1104 20-21 0.14660863824664716 1104 22-23 0.34226317476367996 1104 24-25 0.7235106566721399 1104 26-27 0.3420568205505674 1104 28-29 0.5764220693247779 1104 30-31 0.5681903854312367 1104 32-33 0.5711018876928264 1104 34-35 0.5070036532104112 1104 36-37 0.3790954418536572 1104 38-39 0.04397885947897606 1104 40-41 0.10720770487927922 1104 42-43 0.6693595427311365 1104 44-45 1.0038789072726715 1104 46-47 0.4488716086528157 1104 48-49 0.36501755079161313 1104 50-51 0.7360186373292663 1104 52-53 1.020950440424354 1104 54-55 0.8866924104049687 1104 56-57 0.8672901955737267 1104 58-59 1.2084301641180808 1104 60-61 0.9019757849368162 1104 62-63 1.3326085353793609 1104 64-65 1.6781044607133104 1104 66-67 1.2668882405551187 1104 68-69 1.6278186073700205 1104 70-71 1.5814775407329833 1104 72-73 1.601284458016032 1104 74-75 1.4596693988592797 1104 76-77 1.3246031233127802 1104 78-79 1.4666526149740164 1104 80-81 1.2761445122505322 1104 82-83 1.011435690729293 1104 84-85 0.28269428531852014 1104 86-87 0.4691522098408214 1104 88-89 0.5789339168184746 1104 90-91 0.9650830110516715 1104 92-93 0.8231451387255895 1104 94-95 0.1923221404374118 1104 96-97 0.42740803108916126 1104 98-99 0.6952695019197144 1104 100-101 1.2828505725024506 1105 1 0.17232888976951344 1105 2 0.08829055929945184 1105 3 0.20039347394282458 1105 4 0.1794743204249869 1105 5 0.2776387910676732 1105 6 0.16736591399008205 1105 7 0.4060450076109561 1105 8 0.5821704414836262 1105 9 0.43146004359367396 1105 10-11 0.4421176076641018 1105 12-13 0.40047006044859046 1105 14-15 0.21883891259157195 1105 16-17 0.14593753586747482 1105 18-19 0.14307357550057276 1105 20-21 0.1017321403253959 1105 22-23 -0.02818192183695345 1105 24-25 0.28242889258964254 1105 26-27 -0.4223925150653116 1105 28-29 -0.29852698097276686 1105 30-31 -0.31003982570432953 1105 32-33 -0.680956480286298 1105 34-35 -0.4562106616179946 1105 36-37 -0.5485763605430165 1105 38-39 -0.17508448119111364 1105 40-41 -0.7033409215167339 1105 42-43 -0.5422651413807031 1105 44-45 -0.6210090033819355 1105 46-47 -0.34245878997765544 1105 48-49 -0.2258359586238896 1105 50-51 -0.26995266779831084 1105 52-53 0.015916609821921668 1105 54-55 -0.19894384947927435 1105 56-57 -0.19865909928239134 1105 58-59 -0.02854709193474747 1105 60-61 -0.037067991047532445 1105 62-63 -0.1092884239423988 1105 64-65 -0.17273519038312557 1105 66-67 0.09045961089831422 1105 68-69 -0.045247341924831375 1105 70-71 -0.056636912678609264 1105 72-73 -0.7563416934472436 1105 74-75 -0.35770648766554203 1105 76-77 -0.6051881867059699 1105 78-79 -0.48551580436241437 1105 80-81 -0.13577364214744847 1105 82-83 -0.3883971942466573 1105 84-85 -0.17575113736021208 1105 86-87 -0.3284144323699856 1105 88-89 -0.015384172363884119 1105 90-91 -0.22779218255719869 1105 92-93 -0.3513474022379697 1105 94-95 0.009310809223588734 1105 96-97 -0.04807497209009526 1105 98-99 -0.21108494497031316 1105 100-101 0.06348153361763664 1106 1 0.16756661924182126 1106 2 0.31466483279715973 1106 3 0.4603468753817488 1106 4 0.6066960732477824 1106 5 0.24210098734491226 1106 6 0.37434545670969754 1106 7 0.5334485460291631 1106 8 0.5033241399243664 1106 9 0.4047938889991016 1106 10-11 0.7456931511006175 1106 12-13 0.8010192577755788 1106 14-15 0.7912075020889731 1106 16-17 0.41470497832337827 1106 18-19 0.255799847359647 1106 20-21 0.18322841500086895 1106 22-23 0.25958339551973353 1106 24-25 0.3510401530551164 1106 26-27 0.21824808713147803 1106 28-29 0.49368696275050894 1106 30-31 0.41612321805222763 1106 32-33 0.40481559642209675 1106 34-35 0.8649161530375125 1106 36-37 0.5308652248322048 1106 38-39 0.6133593316977439 1106 40-41 0.44910294257932293 1106 42-43 0.7207862962617142 1106 44-45 0.6598881469368365 1106 46-47 0.37598476687515614 1106 48-49 0.8231693413223411 1106 50-51 0.3815502638144572 1106 52-53 0.6890417858467082 1106 54-55 1.0512289748543928 1106 56-57 0.8593714714219622 1106 58-59 0.7083886686203407 1106 60-61 0.6671527632346717 1106 62-63 0.5955724204644959 1106 64-65 0.5039616885374265 1106 66-67 0.32386847486636583 1106 68-69 0.696002626070662 1106 70-71 0.15210287788390175 1106 72-73 -0.4393050546885071 1106 74-75 -0.04694913303001158 1106 76-77 -0.3967684412058041 1106 78-79 -0.21457205548257008 1106 80-81 -0.2712295143342516 1106 82-83 -0.31666229397269774 1106 84-85 -2.704296765720926E-4 1106 86-87 -0.05493592659637514 1106 88-89 -0.1798873344091021 1106 90-91 -0.1490226918129025 1106 92-93 -0.07909146860251326 1106 94-95 -0.3060871988159448 1106 96-97 -0.8878540129790551 1106 98-99 -0.898932193862958 1106 100-101 -0.9870983880353315 1107 1 0.27811671896159496 1107 2 0.38911384948444194 1107 3 0.2696044048558832 1107 4 0.5610395636923542 1107 5 0.342237313113003 1107 6 0.4027971494213176 1107 7 0.5234134547666329 1107 8 0.42088491849597887 1107 9 0.5423314416991118 1107 10-11 0.46529124253986964 1107 12-13 0.6684361369253082 1107 14-15 0.5305980754145594 1107 16-17 0.3792981468876633 1107 18-19 0.3116744781440275 1107 20-21 0.2602650967306914 1107 22-23 0.391857258840524 1107 24-25 0.44884758028492655 1107 26-27 0.23576847288290992 1107 28-29 0.05512822906542425 1107 30-31 0.27031775997240715 1107 32-33 0.3347529875508286 1107 34-35 0.4865805896839035 1107 36-37 0.8171430264529604 1107 38-39 0.7119115015495439 1107 40-41 0.836583692875962 1107 42-43 0.5098600384040495 1107 44-45 0.41763851930814866 1107 46-47 -0.17639967179087535 1107 48-49 0.2229067879911959 1107 50-51 0.04202967609892383 1107 52-53 0.26254409581111204 1107 54-55 0.34952364047758167 1107 56-57 0.44763609488514433 1107 58-59 0.2675893956911004 1107 60-61 0.4428085154247192 1107 62-63 0.07114107563603866 1107 64-65 -0.20999403083345314 1107 66-67 -0.01871313125315055 1107 68-69 -0.027628436008054535 1107 70-71 -0.09180033082074956 1107 72-73 -0.040451200961005185 1107 74-75 0.05815424734412744 1107 76-77 -0.6873064230795265 1107 78-79 -0.46537991188609595 1107 80-81 -0.35914070062088754 1107 82-83 -0.18209108900438054 1107 84-85 -0.16136835701998464 1107 86-87 -0.2483544214589095 1107 88-89 -0.3267341698840944 1107 90-91 -0.12029329846257042 1107 92-93 0.14504450372839983 1107 94-95 0.4309618532150594 1107 96-97 0.2620475295217659 1107 98-99 0.35614215417167827 1107 100-101 0.5855392631221754 1108 1 0.016980594811773386 1108 2 0.10058046506064855 1108 3 -0.035956472622800106 1108 4 -0.04888475856073171 1108 5 0.23547421319921824 1108 6 0.15823349389876284 1108 7 0.2533684312969555 1108 8 0.12559801362002787 1108 9 -0.09671444392211725 1108 10-11 0.1486900355277001 1108 12-13 0.12168836415013828 1108 14-15 0.3109889174492366 1108 16-17 0.7935499909340322 1108 18-19 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-3.363803772872238 2316 84-85 -3.5159168257925906 2316 86-87 -3.5437788246419366 2316 88-89 -3.6368227881623696 2316 90-91 -2.9467943835843435 2316 92-93 -3.7364812980560203 2316 94-95 -3.4870360971104795 2316 96-97 -3.7148333482211804 2316 98-99 -4.665123218386796 2316 100-101 -4.844304599911343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 421.0 3 161.0 4 149.0 5 143.0 6 152.0 7 212.0 8 295.0 9 413.0 10 593.0 11 1044.0 12 1947.0 13 2754.0 14 3415.0 15 4712.0 16 6946.0 17 9846.0 18 6477.0 19 3491.0 20 3274.0 21 3365.0 22 3646.0 23 4165.0 24 4392.0 25 4605.0 26 4770.0 27 5332.0 28 6149.0 29 7002.0 30 8076.0 31 9771.0 32 11635.0 33 14748.0 34 19136.0 35 27807.0 36 31794.0 37 31637.0 38 20949.0 39 3024.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.312101602345585 17.72191983428893 14.035891647014557 13.930086916350929 2 15.263046126677917 24.49247987243539 38.38972031906055 21.854753681826143 3 23.920762126174054 33.59301359502547 29.563312432239098 12.92291184656138 4 10.81309657757278 17.6784423597088 38.72417159079901 32.7842894719194 5 9.978760666244364 42.89488392890413 30.206058799418713 16.920296605432796 6 23.151315936584602 37.9534595976536 18.094407549026183 20.800816916735613 7 22.811651324539085 41.83911814121215 18.85870962214035 16.490520912108423 8 26.475026547683367 26.97132850196546 19.14190435382007 27.411740596531104 9 29.702173329755837 19.10234624213696 21.75453895000447 29.440941478102733 10-11 24.683198774718505 27.897326777133298 22.35244357575001 25.06703087239819 12-13 33.15410683911162 27.402836697090454 23.359434821732982 16.083621642064944 14-15 27.479138293690347 25.794365709111705 31.702527624643924 15.023968372554025 16-17 17.26272095865502 30.932348645184366 30.804542880138314 21.000387516022297 18-19 16.256371467747705 29.069542148563254 37.90706599499225 16.767020388696793 20-21 21.05783978099265 24.030614812067768 35.80375063128607 19.10779477565351 22-23 18.861418783739982 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19.01662916660458 48-49 18.333109824694723 30.164353352257066 31.606170328483195 19.896366494565015 50-51 17.346642278936525 31.459426838672183 32.75282091781807 18.441109964573222 52-53 17.349476135040746 33.122793946449356 29.64302677532014 19.884703143189757 54-55 15.49739136589609 30.84316344149712 31.379232627577203 22.28021256502959 56-57 18.90365083415442 31.33559698337279 27.968433071144794 21.792319111328 58-59 20.00406052794314 30.083482964408915 28.6688173805497 21.24363912709825 60-61 19.145583471942572 29.828583987885583 31.648354104540854 19.377478435630987 62-63 20.637747024530164 30.158132950883793 30.314962096518837 18.889157928067203 64-65 19.477923988861882 32.4664971735628 30.74660780972071 17.308971027854607 66-67 19.182923012726892 30.55125033531042 29.677021549283182 20.5888051026795 68-69 18.243648655195674 31.796600441994716 30.170797789281067 19.78895311352854 70-71 20.123737467854347 31.010025716521934 28.41638403339421 20.44985278222951 72-73 18.81338062276002 29.292744897883153 30.80605622573747 21.087818253619357 74-75 17.41815763144804 32.91860198646254 30.020885617243582 19.642354764845834 76-77 17.9178302689994 29.80678767509925 30.906706261748578 21.368675794152768 78-79 17.151472120192246 29.587575264579108 32.78973252305691 20.471220092171738 80-81 17.84646470671568 31.115464624698962 30.70519464058038 20.332876028004982 82-83 16.64141738827427 29.744395312392268 31.42500964317459 22.18917765615887 84-85 17.021546119425786 29.26349007641551 30.622091571939002 23.0928722322197 86-87 17.358525012528155 30.00609175638191 30.74138724382488 21.893995987265065 88-89 17.68974118707817 28.216625651782707 30.10318612063778 23.990447040501344 90-91 16.940843716286842 27.36609297238479 31.66255820323199 24.030505108096374 92-93 17.2841829149698 29.84309970304076 31.34987909250448 21.522838289484962 94-95 16.645959030547335 29.257933942951237 31.020473549797984 23.075633476703445 96-97 14.484243783578362 28.878324304587892 32.17431315712997 24.463118754703768 98-99 16.76921643529302 29.114879344079007 29.662536103605703 24.45336811702227 100-101 17.03507660193089 27.847318000521863 31.05173891974503 24.065866477802214 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 668.0 1 573.5 2 862.0 3 1181.0 4 966.5 5 924.0 6 1401.0 7 2541.0 8 3875.5 9 4241.0 10 3345.5 11 2437.0 12 2218.5 13 2170.5 14 2089.0 15 1938.5 16 1739.0 17 1581.0 18 1513.5 19 1486.0 20 1445.0 21 1492.5 22 1575.5 23 1673.0 24 1768.0 25 1885.0 26 2083.0 27 2298.0 28 2615.0 29 2950.0 30 3331.0 31 3618.5 32 4052.5 33 4612.5 34 4963.5 35 5270.0 36 5851.5 37 6575.5 38 6566.5 39 6125.5 40 5685.5 41 5260.5 42 4995.0 43 4982.5 44 5183.0 45 5251.5 46 6169.0 47 11817.0 48 28270.5 49 27761.5 50 12011.5 51 6558.5 52 3728.0 53 2742.0 54 2515.0 55 2362.0 56 2445.0 57 2575.5 58 2635.5 59 2775.0 60 3030.5 61 3372.0 62 2991.0 63 1692.0 64 769.0 65 565.5 66 454.5 67 325.5 68 240.0 69 170.0 70 143.0 71 134.0 72 107.0 73 136.5 74 132.5 75 82.0 76 59.5 77 39.0 78 28.0 79 21.0 80 15.5 81 11.0 82 8.0 83 7.0 84 3.5 85 1.5 86 3.5 87 2.5 88 1.5 89 1.5 90 0.5 91 0.5 92 0.5 93 1.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010802837048515914 2 0.01303790678269162 3 0.015272976516867326 4 0.015645488139229943 5 0.02905590654428418 6 0.04544641792823936 7 0.005587674335439266 8 0.023468232208844916 9 0.038741208725712246 10-11 0.037809929669805696 12-13 0.027007092621289783 14-15 0.0273796042436524 16-17 0.026820836810108474 18-19 0.02384074383120753 20-21 0.05457295267612349 22-23 0.01303790678269162 24-25 0.002793837167719633 26-27 5.587674335439266E-4 28-29 1.8625581118130884E-4 30-31 0.002607581356538324 32-33 0.0020488139229943975 34-35 0.0 36-37 1.8625581118130884E-4 38-39 1.8625581118130884E-4 40-41 0.001303790678269162 42-43 0.003911372034807486 44-45 0.0018625581118130886 46-47 0.0014900464894504707 48-49 7.450232447252353E-4 50-51 0.002607581356538324 52-53 0.003911372034807486 54-55 0.004656395279532721 56-57 9.312790559065443E-4 58-59 0.003725116223626177 60-61 0.002793837167719633 62-63 0.0011175348670878532 64-65 1.8625581118130884E-4 66-67 0.014900464894504709 68-69 0.04432888306115151 70-71 0.05140660388604125 72-73 0.011175348670878531 74-75 0.030359697222553342 76-77 0.024026999642388844 78-79 0.05382792943139826 80-81 0.07822744069614972 82-83 0.04600518536178329 84-85 0.017508046251043033 86-87 0.01955686017403743 88-89 0.018439325306949576 90-91 0.037064906425080466 92-93 0.02272320896411968 94-95 0.010616581237334605 96-97 0.017135534628680414 98-99 0.04581892955060198 100-101 0.06630706878054594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 268448.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.35720209498908 #Duplication Level Percentage of deduplicated Percentage of total 1 79.93399999999309 29.86110592260599 2 10.496999999999094 7.842771007821329 3 3.691999999999681 4.137683704040633 4 1.7339999999998503 2.5910955373082185 5 0.9390000000085377 1.7539206383756845 6 0.610999999999982 1.369515028802259 7 0.3979999999999657 1.0407716503663063 8 0.2859999999999753 0.8547327839332761 9 0.23099999999998005 0.7766562315547558 >10 1.3859999999998802 9.660198889742391 >50 0.13399999999998843 3.6744543980628084 >100 0.1109999999999904 8.113237150989026 >500 0.021999999999998097 5.615908190939223 >1k 0.021999999999998097 15.888018050997482 >5k 0.0029999999999997407 6.819930814460616 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7376 2.747645726546668 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5629 2.0968679222791753 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5251 1.9560585290261057 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3713 1.3831356538323996 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3680 1.3708427702944332 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3612 1.3455119799737751 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2939 1.0948116581237335 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2809 1.046385147216593 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2787 1.0381898915246155 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2338 0.8709321730838002 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2254 0.8396411968053402 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1597 0.5949010609131005 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1567 0.5837257122422219 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1436 0.5349266897127191 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1433 0.5338091548456312 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1404 0.5230063177971153 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1404 0.5230063177971153 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1360 0.5066158064131601 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1323 0.49283287638574325 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1285 0.47867743473596375 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1259 0.4689921325545357 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1137 0.4235457146262963 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1087 0.40492013350816547 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1071 0.39895994755036357 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1035 0.3855495291453093 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 915 0.3408481344617952 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 889 0.33116283228036714 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 882 0.32855525092382887 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 813 0.3028519489808082 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 812 0.30247943735844557 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 743 0.27677613541542495 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 724 0.26969841459053523 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 722 0.26895339134581 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 692 0.2577780426749315 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 680 0.25330790320658003 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 660 0.2458576707593277 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 660 0.2458576707593277 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 653 0.24325008940278936 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 633 0.23579985695553698 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 632 0.23542734533317441 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 602 0.22425199666229584 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 590 0.21978185719394444 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 573 0.21344915961377994 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 565 0.21046906663487902 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 541 0.20152878769817617 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 536 0.1996662295863631 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 516 0.19221599713911075 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 486 0.1810406484682322 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 482 0.17955060197878173 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 446 0.1661401835737275 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 417 0.15533734652521158 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 376 0.14006437000834426 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 357 0.13298664918345451 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 353 0.13149660269400407 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 351 0.13075157944927882 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 349 0.13000655620455356 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 347 0.12926153295982837 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 343 0.12777148647037787 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 316 0.11771367266658721 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 309 0.11510609131004887 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 306 0.11398855644296102 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 305 0.11361604482059841 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 304 0.11324353319823578 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 300 0.11175348670878532 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 295 0.10989092859697222 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 280 0.10430325426153296 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 270 0.10057813803790677 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.725116223626177E-4 0.0 20-21 0.0 0.0 0.0 7.450232447252355E-4 0.0 22-23 0.0 0.0 0.0 9.312790559065443E-4 0.0 24-25 0.0 0.0 0.0 0.0020488139229943975 0.0 26-27 0.0 0.0 0.0 0.0035388604124448686 0.0 28-29 0.0 0.0 0.0 0.008381511503158898 0.0 30-31 0.0 0.0 0.0 0.01620425557277387 0.0 32-33 0.0 0.0 0.0 0.020860650852306595 0.0 34-35 0.0 0.0 0.0 0.027193348432471093 0.0 36-37 0.0 0.0 0.0 0.03389855763499821 0.0 38-39 0.0 0.0 0.0 0.03967248778161879 0.0 40-41 0.0 0.0 0.0 0.057553045655024435 0.0 42-43 0.0 0.0 0.0 0.0737573012277983 0.0 44-45 0.0 0.0 0.0 0.10672457980688999 0.0 46-47 0.0 0.0 0.0 0.19258850876147338 0.0 48-49 0.0 0.0 0.0 0.2505140660388604 0.0 50-51 0.0 0.0 0.0 0.3136547860293241 0.0 52-53 0.0 0.0 0.0 0.37307038979616164 0.0 54-55 0.0 0.0 0.0 0.439004946954345 0.0 56-57 0.0 0.0 0.0 0.5248688759089284 0.0 58-59 0.0 0.0 0.0 0.6444451066873287 0.0 60-61 0.0 0.0 0.0 0.8010862438908093 0.0 62-63 0.0 0.0 0.0 0.9985174037429967 0.0 64-65 0.0 0.0 0.0 1.130572773870545 0.0 66-67 0.0 0.0 0.0 1.3106821432828704 0.0 68-69 0.0 0.0 0.0 1.5416393491476934 0.0 70-71 0.0 0.0 0.0 1.7003293002741686 0.0 72-73 0.0 0.0 0.0 1.970213970675885 0.0 74-75 0.0 0.0 0.0 2.1911133627369175 0.0 76-77 0.0 0.0 0.0 2.336020383835976 0.0 78-79 0.0 0.0 0.0 2.5602723804982714 0.0 80-81 0.0 0.0 0.0 2.770368935510788 0.0 82-83 0.0 0.0 0.0 3.0197654666825606 0.0 84-85 0.0 0.0 0.0 3.421891763023006 0.0 86-87 0.0 0.0 0.0 3.9950008940278936 0.0 88-89 0.0 0.0 0.0 4.582637978304923 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGCGGG 25 0.0050161197 57.002796 4 CGCGGAG 25 0.0050198208 56.992176 8 CGTCACT 75 2.3737812E-9 50.66915 1 GGTTAGG 40 5.379454E-4 47.50233 1 GCTTATG 60 1.487475E-6 47.502327 1 TCTATAG 55 4.951669E-5 43.183937 3 GGGAACT 110 1.6116246E-9 38.858303 7 CACGCAG 50 0.0016132203 38.001865 5 GGGTTAG 50 0.0016132203 38.001865 1 GTAGTTG 50 0.0016132203 38.001865 3 ATGGGCG 65 1.3250909E-4 36.540253 5 ATATGAG 55 0.0025754983 34.54715 5 GTCACTA 110 6.9754606E-8 34.54715 2 GGAGCTT 55 0.0025778532 34.540714 8 GGAACTT 60 0.0039482582 31.66232 8 TGGGAGT 205 1.8189894E-12 30.123426 6 GGATATA 65 0.0058347997 29.232203 1 TCACTAC 130 3.0209412E-7 29.232203 3 ACTACCT 135 4.2029933E-7 28.149529 5 GGATTGA 85 6.392458E-4 27.942547 1 >>END_MODULE