##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936699_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 268448 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.846592263678627 31.0 31.0 34.0 30.0 34.0 2 31.84709887948504 31.0 31.0 34.0 30.0 34.0 3 31.906078644653714 31.0 31.0 34.0 30.0 34.0 4 35.69455909524377 37.0 35.0 37.0 33.0 37.0 5 35.620202050303966 37.0 35.0 37.0 33.0 37.0 6 35.79154994635832 37.0 35.0 37.0 35.0 37.0 7 35.72372675527476 37.0 35.0 37.0 33.0 37.0 8 35.75535299201335 37.0 35.0 37.0 33.0 37.0 9 37.233196000715225 39.0 37.0 39.0 34.0 39.0 10-11 37.14964909405174 39.0 37.0 39.0 33.0 39.0 12-13 37.00060533138634 39.0 37.0 39.0 33.0 39.0 14-15 37.67026947490761 39.0 37.0 41.0 32.0 41.0 16-17 37.790862662415066 39.0 36.5 41.0 32.5 41.0 18-19 38.22927904100608 40.0 37.5 41.0 33.5 41.0 20-21 38.349920655024434 40.0 37.5 41.0 34.0 41.0 22-23 38.228934467755394 40.0 37.0 41.0 33.5 41.0 24-25 38.00862364405769 40.0 37.0 41.0 33.0 41.0 26-27 37.625273796042435 39.5 36.0 41.0 33.0 41.0 28-29 37.432517657050894 39.0 36.0 41.0 33.0 41.0 30-31 37.02178075455954 38.5 35.0 40.0 33.0 41.0 32-33 36.431239942186195 38.0 35.0 40.0 31.5 41.0 34-35 36.009703927762544 38.0 35.0 40.0 31.0 41.0 36-37 35.562188952795324 38.0 35.0 40.0 30.0 41.0 38-39 35.14109250208607 37.0 35.0 40.0 29.5 41.0 40-41 34.69519236500179 37.0 34.5 40.0 26.5 41.0 42-43 34.09137896352366 37.0 33.5 40.0 23.5 41.0 44-45 33.24062388246513 37.0 33.0 40.0 17.5 41.0 46-47 32.06405896113959 36.0 32.5 40.0 2.0 41.0 48-49 31.056811747526524 36.0 31.0 40.0 2.0 41.0 50-51 30.518402074144714 35.0 30.0 40.0 2.0 41.0 52-53 30.170153996304684 35.0 29.0 39.0 2.0 41.0 54-55 29.870960111455478 35.0 28.0 39.0 2.0 41.0 56-57 29.498431726069853 35.0 27.5 39.0 2.0 40.0 58-59 29.16294589641197 35.0 27.0 39.0 2.0 40.0 60-61 28.6214834902849 35.0 26.0 38.0 2.0 40.0 62-63 28.267332965788533 35.0 26.0 38.0 2.0 40.0 64-65 27.927017522946716 34.0 25.5 37.5 2.0 39.5 66-67 27.39107573906306 34.0 23.0 37.0 2.0 39.0 68-69 26.924702363213733 34.0 22.0 36.0 2.0 39.0 70-71 26.319553507569438 33.0 20.5 35.5 2.0 38.5 72-73 25.7086437596853 33.0 16.5 35.0 2.0 37.0 74-75 25.167585528668496 33.0 10.5 35.0 2.0 37.0 76-77 23.74938163070688 30.5 7.0 34.0 2.0 35.0 78-79 24.371645532840624 32.0 2.0 34.5 2.0 36.0 80-81 24.105240120991773 32.0 2.0 35.0 2.0 36.0 82-83 23.652962584932652 31.5 2.0 35.0 2.0 35.0 84-85 23.182830566813685 31.0 2.0 34.0 2.0 35.0 86-87 22.757349654309216 31.0 2.0 34.0 2.0 35.0 88-89 22.121358698891406 30.5 2.0 34.0 2.0 35.0 90-91 21.39681800572178 29.0 2.0 34.0 2.0 35.0 92-93 20.874666602097985 29.0 2.0 34.0 2.0 35.0 94-95 20.209798545714627 29.0 2.0 34.0 2.0 35.0 96-97 19.401571254023125 27.0 2.0 34.0 2.0 35.0 98-99 18.570188639885565 25.5 2.0 34.0 2.0 35.0 100-101 15.7823693974252 11.0 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11794527274174627 1101 2 0.20170725806954337 1101 3 -0.04614980683866676 1101 4 0.050661221627656516 1101 5 -0.07001944347771172 1101 6 0.0633775248816093 1101 7 0.17906565785607143 1101 8 -0.07039742768623114 1101 9 0.108593131689112 1101 10-11 -0.050190263836057625 1101 12-13 0.043076293951692435 1101 14-15 -0.2346550819727753 1101 16-17 -0.17380244870877704 1101 18-19 0.18946806075189215 1101 20-21 0.01117781213198299 1101 22-23 0.23318796284981858 1101 24-25 0.3950699632703163 1101 26-27 -0.13375093458282095 1101 28-29 0.02544944384261072 1101 30-31 0.26291011598284797 1101 32-33 0.32717695563204785 1101 34-35 0.6617915350709467 1101 36-37 0.9163209123593106 1101 38-39 0.9164357634605125 1101 40-41 0.7871376424872807 1101 42-43 0.5543721744628627 1101 44-45 0.15662245767816074 1101 46-47 0.549030251666899 1101 48-49 0.532676811626061 1101 50-51 0.7060882236033628 1101 52-53 1.204319913545664 1101 54-55 1.4135052943557405 1101 56-57 1.7845435512867631 1101 58-59 1.5102212130948587 1101 60-61 1.342334461263821 1101 62-63 1.3256094338918167 1101 64-65 1.3795441925413172 1101 66-67 1.5322517060992809 1101 68-69 1.6946676010592725 1101 70-71 1.7648692809281528 1101 72-73 1.7366420134995089 1101 74-75 1.9003631270301682 1101 76-77 1.820229753474365 1101 78-79 2.002084266933533 1101 80-81 1.8488071207529337 1101 82-83 1.6152506743818194 1101 84-85 1.690400657836289 1101 86-87 1.8641902234090146 1101 88-89 1.9500641147157936 1101 90-91 2.2199407717884263 1101 92-93 1.9652893322382106 1101 94-95 1.94204896864483 1101 96-97 2.3663104943686513 1101 98-99 1.9814460937182723 1101 100-101 1.274685805027536 1102 1 0.23257433332120314 1102 2 0.41433895253836894 1102 3 0.198747032494083 1102 4 0.08374945692177249 1102 5 0.15018468294898213 1102 6 0.07687620793691963 1102 7 0.1036157017542294 1102 8 0.21373338881947035 1102 9 0.1000329912150093 1102 10-11 0.5292653990261016 1102 12-13 0.4854050911422121 1102 14-15 0.7131884211000994 1102 16-17 0.5547423274106293 1102 18-19 0.3966124856860489 1102 20-21 0.08646315014778594 1102 22-23 0.42708063185772716 1102 24-25 0.3285642565099991 1102 26-27 0.5227459925462341 1102 28-29 0.1676794701815041 1102 30-31 0.19105432142622192 1102 32-33 0.3820222146311707 1102 34-35 0.464002904693416 1102 36-37 0.7309883838255118 1102 38-39 0.7644932700452358 1102 40-41 0.9557065625926384 1102 42-43 0.43025013758840913 1102 44-45 0.4414666192233767 1102 46-47 0.6143561954772636 1102 48-49 1.4643603059192998 1102 50-51 1.433672727554196 1102 52-53 1.159379176056639 1102 54-55 0.9271137228017423 1102 56-57 0.9673244994869385 1102 58-59 0.764336665245871 1102 60-61 0.7850199309740944 1102 62-63 0.7605029808628458 1102 64-65 0.7894980994772247 1102 66-67 0.5927762890667978 1102 68-69 0.2123091726132671 1102 70-71 0.03325163386933028 1102 72-73 0.005518220698807141 1102 74-75 0.10539495319347125 1102 76-77 -0.2895979462622478 1102 78-79 -0.2079135381586532 1102 80-81 -0.586333353347154 1102 82-83 -0.3959982281642773 1102 84-85 -0.5187191841303509 1102 86-87 0.14140708030102545 1102 88-89 0.12859242902659318 1102 90-91 0.038174968452167946 1102 92-93 0.39041553943750884 1102 94-95 0.6458187228151537 1102 96-97 0.6062962274678618 1102 98-99 -0.2551100959217649 1102 100-101 -0.2766201651217184 1103 1 0.36200964837249217 1103 2 0.29945251753732904 1103 3 0.40220893326862495 1103 4 0.1832234992077275 1103 5 0.2330426481991381 1103 6 0.20637694061433365 1103 7 0.2593227354573884 1103 8 0.18644115520004334 1103 9 0.32098756521543237 1103 10-11 0.16952078943849358 1103 12-13 0.3149398409852111 1103 14-15 0.26541131203583035 1103 16-17 0.2760501565921274 1103 18-19 0.3518014117902979 1103 20-21 0.299619943114088 1103 22-23 0.479722195600651 1103 24-25 0.4887851064157971 1103 26-27 0.5218301457804841 1103 28-29 0.3621268220760001 1103 30-31 0.40385131744883296 1103 32-33 0.47480403376452074 1103 34-35 0.344726946979371 1103 36-37 0.5276769435910538 1103 38-39 0.5229583108658389 1103 40-41 0.6377245655233494 1103 42-43 0.7057106776763291 1103 44-45 0.30093647478125973 1103 46-47 0.3755360677820576 1103 48-49 0.40361349829945325 1103 50-51 0.49989499048071906 1103 52-53 0.5976380205196925 1103 54-55 0.5341631261929933 1103 56-57 0.36266938121187664 1103 58-59 0.6125808561606689 1103 60-61 0.6388965794982724 1103 62-63 0.7680325496298543 1103 64-65 0.5246482983468077 1103 66-67 0.5838664083885483 1103 68-69 0.6051485592576036 1103 70-71 1.0513461492555543 1103 72-73 1.1941564873933856 1103 74-75 0.6579187652093772 1103 76-77 0.7273910112424602 1103 78-79 0.8966107448641658 1103 80-81 0.834877657747672 1103 82-83 0.4842220984005152 1103 84-85 0.3634898382686451 1103 86-87 0.2474098016742694 1103 88-89 0.33509624941595817 1103 90-91 0.5249017321113669 1103 92-93 0.40367569225308486 1103 94-95 0.49278727002051426 1103 96-97 -0.07044257303454415 1103 98-99 -0.4593570082098104 1103 100-101 -0.25993867045979613 1104 1 0.3236409323746159 1104 2 0.28709926355933035 1104 3 0.3520459957575497 1104 4 0.3159868921257427 1104 5 0.20462743706211484 1104 6 0.15082820252786178 1104 7 0.3367828191759088 1104 8 0.13555556596619311 1104 9 0.19795951814762702 1104 10-11 0.27808216711265743 1104 12-13 0.25697090137435197 1104 14-15 0.3590287974805335 1104 16-17 0.2211695304757626 1104 18-19 0.05689815167657741 1104 20-21 -0.07631575451752326 1104 22-23 0.09174003009977838 1104 24-25 0.1547700261741909 1104 26-27 0.36579873088295045 1104 28-29 0.12764679044294525 1104 30-31 0.3063137872815318 1104 32-33 0.5585702763846072 1104 34-35 0.37404206403531504 1104 36-37 0.41188904868915444 1104 38-39 0.6197868242485285 1104 40-41 0.7728127153985866 1104 42-43 0.5843801804101432 1104 44-45 0.3373281247451203 1104 46-47 0.29387642310855 1104 48-49 0.7477866074744028 1104 50-51 0.7618153351719599 1104 52-53 0.9960509137172195 1104 54-55 0.9016178922856284 1104 56-57 1.115467952270354 1104 58-59 1.022351652850105 1104 60-61 1.2514598115240183 1104 62-63 1.650765263936993 1104 64-65 1.374704883340197 1104 66-67 1.4889195164727056 1104 68-69 1.6596201372289947 1104 70-71 1.6269382464453948 1104 72-73 1.797522953626462 1104 74-75 1.7874506777821573 1104 76-77 1.6507967460974555 1104 78-79 1.4118886930786694 1104 80-81 1.2525499011040502 1104 82-83 1.2109574851555358 1104 84-85 1.048795465407629 1104 86-87 1.4376376414910155 1104 88-89 1.3366369974286663 1104 90-91 1.702299095114327 1104 92-93 1.3772239122119068 1104 94-95 1.7356527886253872 1104 96-97 1.5555510730842705 1104 98-99 1.4214872671613996 1104 100-101 0.7308032065257937 1105 1 0.23364337796033396 1105 2 0.3734284393969425 1105 3 0.0926508706250786 1105 4 0.29356902304609633 1105 5 0.3932228556648738 1105 6 0.3766780435647874 1105 7 0.1424402741363764 1105 8 0.25404419399260547 1105 9 0.23395038796094525 1105 10-11 0.31733381788301074 1105 12-13 0.36700187879343815 1105 14-15 0.1451215666987622 1105 16-17 0.3113912635808447 1105 18-19 0.0891187435083225 1105 20-21 0.1642687404690193 1105 22-23 0.10494619297578112 1105 24-25 0.21222091066102422 1105 26-27 0.2606996846613754 1105 28-29 0.18911251148313113 1105 30-31 0.007035235068272527 1105 32-33 -0.012494368597870675 1105 34-35 -0.18499845116807023 1105 36-37 0.0025387592948433735 1105 38-39 -0.4564339306222607 1105 40-41 -0.4700339463769012 1105 42-43 -0.7068483188154246 1105 44-45 -1.1565986707223885 1105 46-47 -1.1541956401506042 1105 48-49 -1.383235647355626 1105 50-51 -1.0087683446193552 1105 52-53 -1.1317920796797019 1105 54-55 -0.8180364649329235 1105 56-57 -0.9255363682644138 1105 58-59 -0.8774812321258452 1105 60-61 -0.6454791303525624 1105 62-63 -0.9021612869719497 1105 64-65 -0.7994411996466795 1105 66-67 -1.0293816166486778 1105 68-69 -0.8486133346875278 1105 70-71 -1.1198824921214907 1105 72-73 -1.0551305068773296 1105 74-75 -0.945593261241239 1105 76-77 -0.9193304243598703 1105 78-79 -1.0809579686968256 1105 80-81 -1.1219507918119014 1105 82-83 -1.1541073228659862 1105 84-85 -0.746060652632643 1105 86-87 -0.42424790509739196 1105 88-89 -0.7584147674690236 1105 90-91 -0.8360558408928469 1105 92-93 -1.189071840620894 1105 94-95 -0.6918559747234383 1105 96-97 -0.32441142793472366 1105 98-99 -0.21784997910907933 1105 100-101 0.3222273526087793 1106 1 0.03275749322776633 1106 2 0.02708847472992204 1106 3 -0.02673873943201599 1106 4 0.0010222324579842734 1106 5 0.25223660480074983 1106 6 0.0724432072998411 1106 7 0.02049030358052306 1106 8 -0.020260028041853673 1106 9 0.0544819188004837 1106 10-11 0.2083904786608457 1106 12-13 0.08488215633651919 1106 14-15 0.03864897535790135 1106 16-17 0.06354196639980358 1106 18-19 -0.0171298162889002 1106 20-21 0.1702919885423526 1106 22-23 0.1502901774087988 1106 24-25 0.23414399743163727 1106 26-27 0.17365382860484146 1106 28-29 -0.07050804309095327 1106 30-31 -0.11741748787511597 1106 32-33 -0.08052954795112299 1106 34-35 0.054222431132153304 1106 36-37 -0.10100221927493891 1106 38-39 0.1249599296332562 1106 40-41 0.12527547318936882 1106 42-43 -0.031233192205597504 1106 44-45 0.31573663408853037 1106 46-47 -0.17848065948132685 1106 48-49 -0.433693951884905 1106 50-51 -0.21753373878685167 1106 52-53 -0.5817946400401972 1106 54-55 -0.5135583944205955 1106 56-57 -0.5214602743503924 1106 58-59 -0.606588690455979 1106 60-61 -0.5135373529503049 1106 62-63 -0.2176041013202088 1106 64-65 -0.6212977170443281 1106 66-67 -0.835831164745052 1106 68-69 -0.7564275549297363 1106 70-71 -0.437296784053796 1106 72-73 -0.3917795070565546 1106 74-75 -0.22019832563430697 1106 76-77 -0.9368624928751181 1106 78-79 -0.9239758656167112 1106 80-81 -0.46547435993029396 1106 82-83 -0.5102342655395127 1106 84-85 -0.45393632152682173 1106 86-87 -0.620352316598531 1106 88-89 -0.42670726305240336 1106 90-91 -0.5449172485790506 1106 92-93 -0.4569047514947471 1106 94-95 -0.5477268813470886 1106 96-97 -0.40265604477677996 1106 98-99 -0.11249754548642699 1106 100-101 -0.09453532757659211 1107 1 0.12076335018668871 1107 2 0.25856457985223713 1107 3 0.07536673631966906 1107 4 0.055905976872409724 1107 5 0.12414087381693406 1107 6 0.23128797264280365 1107 7 0.11315395761601366 1107 8 0.11063795484168537 1107 9 0.24853155147827977 1107 10-11 0.23566194365794502 1107 12-13 0.33734359381600854 1107 14-15 0.4381087213880477 1107 16-17 0.5097248448931495 1107 18-19 0.0899742709596012 1107 20-21 0.24072065734647197 1107 22-23 0.09708525958293279 1107 24-25 0.3665630224499452 1107 26-27 0.5701030472562252 1107 28-29 0.3325807453768945 1107 30-31 0.5681496525656726 1107 32-33 0.09861260953038453 1107 34-35 -0.14098218374755334 1107 36-37 -0.25185046849863824 1107 38-39 -0.16915029227480005 1107 40-41 -0.05961634978909558 1107 42-43 -0.2985137455872362 1107 44-45 -0.2974011306737836 1107 46-47 0.3233622629392059 1107 48-49 0.06809847951041093 1107 50-51 0.0026386880642768062 1107 52-53 0.3721259880681558 1107 54-55 0.15094136574703398 1107 56-57 0.1515850918357664 1107 58-59 0.20877825306980924 1107 60-61 -0.04281583619991025 1107 62-63 -0.09900699445595151 1107 64-65 0.2013399341131752 1107 66-67 0.22134992131690012 1107 68-69 0.7142245366609821 1107 70-71 0.43270144876031935 1107 72-73 0.3497695575972024 1107 74-75 -0.0761789875716481 1107 76-77 0.13865945809809688 1107 78-79 0.2606950591264159 1107 80-81 0.4515625742546554 1107 82-83 0.5802204061425975 1107 84-85 0.6975241313776728 1107 86-87 0.367908520037755 1107 88-89 0.37757690043753556 1107 90-91 0.2803206903773017 1107 92-93 0.3380322609512909 1107 94-95 0.8027628815975447 1107 96-97 0.5970536318282065 1107 98-99 0.7942265967972837 1107 100-101 0.5280127020111482 1108 1 -0.07357997771828195 1108 2 0.04422412578575319 1108 3 -0.2356304856364595 1108 4 -0.24043466878330122 1108 5 0.088792660386531 1108 6 0.022383863053761388 1108 7 0.11011247854713702 1108 8 0.14775836867443104 1108 9 -0.14472929726202466 1108 10-11 -0.07015039468863904 1108 12-13 0.02725642071514045 1108 14-15 -0.42513248945591187 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-1.0769520146047604 2316 82-83 -1.0052781470283705 2316 84-85 -1.0900934234812603 2316 86-87 -1.444546266554962 2316 88-89 -1.6557001673222906 2316 90-91 -1.8728924757669034 2316 92-93 -1.8502665092440331 2316 94-95 -2.8007456788075658 2316 96-97 -2.913797585919564 2316 98-99 -2.3004954914592872 2316 100-101 -2.179637406501028 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 15.0 6 54.0 7 125.0 8 234.0 9 437.0 10 756.0 11 1374.0 12 2108.0 13 3042.0 14 3781.0 15 5523.0 16 7641.0 17 10271.0 18 8101.0 19 3894.0 20 3129.0 21 3283.0 22 3649.0 23 4027.0 24 4452.0 25 4717.0 26 5096.0 27 5932.0 28 6844.0 29 7835.0 30 9101.0 31 10514.0 32 12609.0 33 15533.0 34 20809.0 35 27491.0 36 31446.0 37 30874.0 38 13227.0 39 517.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.0450753864323 18.895971566629534 14.8943621305655 13.16459091637266 2 15.392152114711898 26.65285792518692 36.86822213695242 21.086767823148765 3 25.00214202960099 32.67992117331068 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24.977369834641475 38.10957097676636 18.640923534254433 24-25 19.63292567992937 25.587546145049117 38.734834064079685 16.044694110941823 26-27 19.5161065012712 22.894793209100474 42.322428035276914 15.266672254351409 28-29 16.474729695750565 27.015151938460964 41.144544091489024 15.365574274299442 30-31 17.043869239752578 27.010510513307167 39.45176953981348 16.49385070712677 32-33 16.47627473806752 27.451827706635623 39.337462165308494 16.73443538998836 34-35 18.504011071189762 25.32143515698776 39.353013378929354 16.82154039289313 36-37 18.609095379296985 25.417778009566238 40.46188404285437 15.511242568282398 38-39 18.782561724083337 26.338278710662827 39.957717489499224 14.921442075754612 40-41 16.74070348950912 28.11797465053688 39.258327978468785 15.882993881485207 42-43 15.565700364319083 30.20532993600203 36.15084598019713 18.07812371948176 44-45 17.583553591086126 28.238999685213024 34.921796856228035 19.25564986747282 46-47 18.805876743354393 29.78323548694719 33.164523483132676 18.246364286565743 48-49 19.023032822610386 29.457091449581473 32.34988433298689 19.16999139482125 50-51 17.594237882902515 31.015522848425924 33.360527188267156 18.029712080404405 52-53 17.835736663102956 32.753765175248745 30.269103398436194 19.141394763212105 54-55 15.789895212090283 30.6732800143045 31.381427246346576 22.15539752725864 56-57 19.12184654015882 30.927051530183615 28.70866250272432 21.24243942693324 58-59 20.80825803759851 29.475323301352436 28.95900969112451 20.757408969924544 60-61 19.073420777159804 30.01110121852637 31.863849887683983 19.05162811662985 62-63 20.719321282559903 29.725551551048156 30.900269140148446 18.65485802624349 64-65 19.80721769499418 32.35222351571595 30.49033760186263 17.35022118742724 66-67 19.660119129660604 30.272828528536362 29.841644719441828 20.22540762236121 68-69 18.87856594720647 31.71746597030271 29.853562408266964 19.550405674223857 70-71 20.414218818103585 31.684815205284 28.24820346231648 19.652762514295933 72-73 19.246278984434227 29.191462571919768 30.723826234021196 20.838432209624816 74-75 17.892134948664072 33.305206610338715 29.513873366414828 19.288785074582385 76-77 18.55392827940226 29.897241329213305 30.553242849080537 20.995587542303902 78-79 17.68770967099001 29.701054106058827 32.27252152823957 20.338714694711587 80-81 18.17656897842713 31.331699194230346 30.4868145090691 20.004917318273424 82-83 17.198070332290268 29.98007003427209 31.019594695276414 21.80226493816123 84-85 17.090683549113503 29.80727859948705 30.192274380737917 22.909763470661535 86-87 17.448541888234715 30.19316698113032 29.64706189128933 22.71122923934564 88-89 17.839454778787932 28.687628403208894 29.403613077709668 24.06930374029351 90-91 17.419763434851447 27.36909751327186 30.78215516438484 24.42898388749185 92-93 17.51327087485332 30.13187058801617 30.460802026485872 21.89405651064464 94-95 16.82926920125463 29.812513503646915 29.761292773965714 23.596924521132742 96-97 14.546260528148947 29.096059929893052 30.930686504226163 25.426993037731844 98-99 16.984762326944104 29.203584326858422 28.393367020989285 25.41828632520819 100-101 16.932367014787207 28.081723578507795 29.423170411430423 25.562738995274575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1677.0 1 1208.0 2 1205.0 3 1550.5 4 1241.0 5 1119.5 6 1553.0 7 2644.5 8 3649.5 9 3854.0 10 3193.0 11 2420.5 12 2189.5 13 2084.0 14 1964.5 15 1798.5 16 1670.5 17 1647.0 18 1594.0 19 1538.0 20 1511.0 21 1502.0 22 1533.0 23 1648.0 24 1735.0 25 1828.0 26 2005.0 27 2244.5 28 2557.5 29 2824.0 30 3180.0 31 3475.5 32 3804.5 33 4372.0 34 4857.0 35 5150.5 36 5751.0 37 6320.0 38 6369.0 39 6241.5 40 5923.0 41 5534.0 42 5225.0 43 5093.5 44 5199.0 45 5290.5 46 6106.5 47 11897.0 48 29123.0 49 28025.5 50 11684.5 51 6470.0 52 3422.5 53 2479.0 54 2257.0 55 2171.0 56 2388.0 57 2632.0 58 2748.0 59 2959.0 60 3094.5 61 3128.0 62 2673.5 63 1539.5 64 745.5 65 534.0 66 419.0 67 327.5 68 256.0 69 192.5 70 163.5 71 152.5 72 129.5 73 138.5 74 123.0 75 90.0 76 71.5 77 49.0 78 35.0 79 28.5 80 18.5 81 10.5 82 9.5 83 6.0 84 2.5 85 3.5 86 3.5 87 1.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011547860293241148 2 0.007077720824889736 3 0.004097627845988795 4 0.0 5 0.005587674335439266 6 0.0 7 7.450232447252353E-4 8 0.002607581356538324 9 0.0 10-11 0.0 12-13 0.0 14-15 7.450232447252353E-4 16-17 0.004470139468351413 18-19 0.0011175348670878532 20-21 7.450232447252353E-4 22-23 3.7251162236261767E-4 24-25 3.7251162236261767E-4 26-27 1.8625581118130884E-4 28-29 1.8625581118130884E-4 30-31 9.312790559065443E-4 32-33 0.003911372034807486 34-35 0.001303790678269162 36-37 0.0014900464894504707 38-39 0.005773930146620574 40-41 1.8625581118130884E-4 42-43 7.450232447252353E-4 44-45 0.004656395279532721 46-47 0.0 48-49 0.0018625581118130886 50-51 0.0018625581118130886 52-53 3.7251162236261767E-4 54-55 3.7251162236261767E-4 56-57 0.012106627726785075 58-59 0.0024213255453570154 60-61 0.0033526046012635593 62-63 1.8625581118130884E-4 64-65 0.003911372034807486 66-67 3.7251162236261767E-4 68-69 0.0022350697341757065 70-71 0.005587674335439266 72-73 0.0024213255453570154 74-75 0.007450232447252354 76-77 0.001303790678269162 78-79 0.00800899988079628 80-81 0.0033526046012635593 82-83 0.0029800929789009414 84-85 0.001303790678269162 86-87 5.587674335439266E-4 88-89 0.0024213255453570154 90-91 0.008567767314340207 92-93 0.0011175348670878532 94-95 7.450232447252353E-4 96-97 3.7251162236261767E-4 98-99 5.587674335439266E-4 100-101 0.0024213255453570154 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 268448.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.08016450113243 #Duplication Level Percentage of deduplicated Percentage of total 1 79.2932081722805 27.816187865061394 2 10.720808732956717 7.521754678745977 3 3.7378413965934674 3.9337227321492434 4 1.7468037208512084 2.4511264751460247 5 1.0002973282929108 1.7545297413279295 6 0.6774837531325659 1.4259744904041007 7 0.4757252686573504 1.168196447729169 8 0.32812300896232427 0.920848730480391 9 0.23042942700590408 0.7275151984741924 >10 1.4654037293463025 9.798173203003934 >50 0.13804527885146328 3.438282274406961 >100 0.1337977318098798 8.856836333293598 >500 0.02442339548910504 6.023512933603528 >1k 0.02442339548910504 16.751102634402194 >5k 0.0031856602811876143 7.412236261771367 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7096 2.643342472285135 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6758 2.5174335439265705 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6044 2.2514602455596613 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4225 1.57386160448206 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3731 1.3898408630349266 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3610 1.34476695672905 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3310 1.2330134700202646 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3006 1.1197699368220289 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2522 0.9394743115985218 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1982 0.7383180355227084 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1931 0.7193199427822148 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1774 0.6608356180712838 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1768 0.6586005483371081 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1709 0.6366223626177137 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1624 0.6049588747168911 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1487 0.5539247824532125 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1420 0.5289665037549172 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1349 0.5025181785671713 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1334 0.496930504231732 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1323 0.49283287638574325 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1256 0.4678745976874479 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1205 0.44887650494695436 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1171 0.43621110978662536 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1143 0.42578078436047206 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1082 0.40305757539635234 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1006 0.37474669209679345 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 977 0.3639438550482775 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 977 0.3639438550482775 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 951 0.35425855286684943 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 931 0.3468083204195971 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 920 0.3427106925736083 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 874 0.32557515794492786 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 769 0.28646143759685305 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 766 0.2853439027297652 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 685 0.2551704613183931 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 675 0.25144534509476696 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 669 0.24921027536059123 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 660 0.2458576707593277 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 659 0.2454851591369651 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 627 0.23356478722136131 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 627 0.23356478722136131 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 588 0.2190368339492192 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 586 0.21829181070449397 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 569 0.2119591131243295 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 554 0.20637143878889022 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 546 0.20339134580998927 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 528 0.19668613660746215 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 524 0.19519609011801167 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 508 0.1892359041602098 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 497 0.185138276314221 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 473 0.17619799737751818 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 451 0.16800274168554058 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 450 0.16763023006317795 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 376 0.14006437000834426 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 339 0.12628143998092742 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 321 0.1195762307784003 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 314 0.11696864942186197 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 310 0.11547860293241148 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 309 0.11510609131004887 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 301 0.11212599833114792 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 298 0.11100846346406008 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 297 0.11063595184169746 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 296 0.11026344021933485 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 295 0.10989092859697222 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 292 0.10877339372988439 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 288 0.1072833472404339 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 282 0.1050482775062582 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 277 0.10318571939444511 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 276 0.1028132077720825 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 273 0.10169567290499464 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.725116223626177E-4 0.0 20-21 0.0 0.0 0.0 7.450232447252355E-4 0.0 22-23 0.0 0.0 0.0 9.312790559065443E-4 0.0 24-25 0.0 0.0 0.0 0.0016763023006317796 0.0 26-27 0.0 0.0 0.0 0.0031663487900822507 0.0 28-29 0.0 0.0 0.0 0.00800899988079628 0.0 30-31 0.0 0.0 0.0 0.015459232328048635 0.0 32-33 0.0 0.0 0.0 0.020115627607581357 0.0 34-35 0.0 0.0 0.0 0.02626206937656455 0.0 36-37 0.0 0.0 0.0 0.03278102276791036 0.0 38-39 0.0 0.0 0.0 0.03874120872571224 0.0 40-41 0.0 0.0 0.0 0.05699427822148051 0.0 42-43 0.0 0.0 0.0 0.0737573012277983 0.0 44-45 0.0 0.0 0.0 0.1070970914292526 0.0 46-47 0.0 0.0 0.0 0.1957548575515556 0.0 48-49 0.0 0.0 0.0 0.2549842055072118 0.0 50-51 0.0 0.0 0.0 0.3181249254976755 0.0 52-53 0.0 0.0 0.0 0.37679550601978784 0.0 54-55 0.0 0.0 0.0 0.4440338538562403 0.0 56-57 0.0 0.0 0.0 0.5330641316009059 0.0 58-59 0.0 0.0 0.0 0.6559929669805697 0.0 60-61 0.0 0.0 0.0 0.8158004529741327 0.0 62-63 0.0 0.0 0.0 1.017329240672309 0.0 64-65 0.0 0.0 0.0 1.1521784479675765 0.0 66-67 0.0 0.0 0.0 1.335826677792347 0.0 68-69 0.0 0.0 0.0 1.5699502324472525 0.0 70-71 0.0 0.0 0.0 1.7334828346644415 0.0 72-73 0.0 0.0 0.0 2.007465132912147 0.0 74-75 0.0 0.0 0.0 2.233020920252712 0.0 76-77 0.0 0.0 0.0 2.3814668017642147 0.0 78-79 0.0 0.0 0.0 2.6122377518178563 0.0 80-81 0.0 0.0 0.0 2.8266181904875434 0.0 82-83 0.0 0.0 0.0 3.0788085588270353 0.0 84-85 0.0 0.0 0.0 3.4805623435451185 0.0 86-87 0.0 0.0 0.0 4.059445404696627 0.0 88-89 0.0 0.0 0.0 4.645033675050661 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGTAC 25 0.0050173667 56.99922 7 TGGGGTA 110 3.45608E-11 43.181225 6 GGTGTGC 45 9.612521E-4 42.221645 8 GTAAGGT 45 9.612521E-4 42.221645 4 ATGGGGT 175 0.0 37.99948 5 TAAGGTG 50 0.0016137183 37.99948 5 GTAAGCG 40 1.07664E-5 35.62451 94-95 GTACACG 55 0.002576292 34.544983 1 TACCTGG 85 1.6277172E-5 33.528954 2 CCATGCA 60 0.0039458703 31.666233 9 AGGTGTG 60 0.0039458703 31.666233 7 ACTACCT 155 1.3478711E-9 30.644741 5 TACACTG 95 3.481312E-5 29.999588 5 CATGGGT 175 1.5097612E-10 29.856735 4 CATGGGG 1020 0.0 29.80351 4 CTTATGT 80 4.4849282E-4 29.687094 2 GGGAGTC 65 0.00583659 29.23037 7 TATTAGT 65 0.00583659 29.23037 4 GCTTATG 100 4.939628E-5 28.49961 1 GTCACTA 150 3.317109E-8 28.499609 2 >>END_MODULE