##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936698_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 271496 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.681870082800483 31.0 31.0 34.0 30.0 34.0 2 31.56306538586204 31.0 31.0 34.0 30.0 34.0 3 31.535134955947786 31.0 31.0 34.0 28.0 34.0 4 35.24301647169756 37.0 35.0 37.0 33.0 37.0 5 35.226172761292986 37.0 35.0 37.0 33.0 37.0 6 35.47975660783216 37.0 35.0 37.0 33.0 37.0 7 35.37757094027168 37.0 35.0 37.0 33.0 37.0 8 35.38559315791024 37.0 35.0 37.0 33.0 37.0 9 36.822067360108434 39.0 37.0 39.0 33.0 39.0 10-11 36.70561813065386 39.0 37.0 39.0 32.0 39.0 12-13 36.61359099213248 39.0 37.0 39.0 32.0 39.0 14-15 37.34053540383652 39.0 36.5 41.0 32.0 41.0 16-17 37.42951277366886 39.0 36.5 41.0 32.0 41.0 18-19 37.71377663022659 40.0 37.0 41.0 32.5 41.0 20-21 37.78111648053746 40.0 37.0 41.0 33.0 41.0 22-23 37.665930621445625 40.0 37.0 41.0 32.5 41.0 24-25 37.436299245661075 39.5 36.0 41.0 32.0 41.0 26-27 37.14767252556207 39.0 35.5 41.0 31.5 41.0 28-29 37.1078561010107 39.0 36.0 41.0 32.0 41.0 30-31 36.817575581224034 38.5 35.0 40.0 32.0 41.0 32-33 36.335881559949314 38.0 35.0 40.0 31.0 41.0 34-35 35.96089997642691 38.0 35.0 40.0 30.5 41.0 36-37 35.53205019595132 38.0 35.0 40.0 30.0 41.0 38-39 35.139816056221825 37.5 35.0 40.0 29.5 41.0 40-41 34.837533517989215 37.0 35.0 40.0 27.5 41.0 42-43 34.282293293455524 37.0 34.5 40.0 24.5 41.0 44-45 33.49967955328992 37.0 33.0 40.0 20.0 41.0 46-47 32.46728128591214 36.0 33.0 40.0 5.5 41.0 48-49 31.58609703273713 36.0 31.5 40.0 2.0 41.0 50-51 30.56288122108613 35.5 29.5 39.0 2.0 40.0 52-53 30.641561938297436 35.0 30.5 39.0 2.0 40.0 54-55 30.932829581282967 35.0 31.0 39.5 2.0 41.0 56-57 30.736412322833488 35.0 31.0 40.0 2.0 41.0 58-59 30.459984677490645 35.0 31.0 39.0 2.0 41.0 60-61 29.978515337242538 35.0 29.0 39.0 2.0 41.0 62-63 29.58613570734007 35.0 28.0 39.0 2.0 40.5 64-65 29.144495683177652 35.0 28.0 38.0 2.0 40.0 66-67 28.59679516456964 35.0 26.5 37.5 2.0 40.0 68-69 28.146479137814186 35.0 26.0 37.0 2.0 39.0 70-71 27.59955579456051 34.5 25.0 36.0 2.0 39.0 72-73 27.087546409523526 34.0 23.5 36.0 2.0 38.5 74-75 26.644749830568408 34.0 22.0 35.0 2.0 37.5 76-77 26.144033429590124 34.0 19.5 35.0 2.0 37.0 78-79 25.638665026372394 33.5 15.0 35.0 2.0 36.5 80-81 25.106436190588443 33.0 7.0 35.0 2.0 36.0 82-83 24.666024545481335 33.0 2.0 35.0 2.0 36.0 84-85 24.200780121990746 32.5 2.0 35.0 2.0 35.0 86-87 23.82555175766862 32.0 2.0 35.0 2.0 35.0 88-89 23.263164098181925 31.5 2.0 35.0 2.0 35.0 90-91 22.69526070365678 31.0 2.0 35.0 2.0 35.0 92-93 22.268532501399655 31.0 2.0 35.0 2.0 35.0 94-95 21.798157247252263 31.0 2.0 35.0 2.0 35.0 96-97 21.168752025812537 30.5 2.0 34.0 2.0 35.0 98-99 20.524458923888382 29.5 2.0 34.0 2.0 35.0 100-101 18.919939888617144 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22864382987692977 1101 2 0.018598584255897777 1101 3 0.1389776564690237 1101 4 0.0545214784637551 1101 5 0.025600769283407487 1101 6 0.27410375117966623 1101 7 0.31471408009868185 1101 8 0.17726212189665347 1101 9 0.28772432679452464 1101 10-11 0.026553224576808532 1101 12-13 0.2433697615568633 1101 14-15 0.31366505099816067 1101 16-17 0.05511637397830782 1101 18-19 0.3127523469956657 1101 20-21 0.25652348753681764 1101 22-23 0.2901041986951398 1101 24-25 0.21917624823382198 1101 26-27 0.5968871226097718 1101 28-29 0.6560204393431377 1101 30-31 0.44856729697171005 1101 32-33 0.37652729548186414 1101 34-35 0.5600574729141812 1101 36-37 0.544674917567896 1101 38-39 0.6697151161747641 1101 40-41 0.7383759976608459 1101 42-43 0.8226391486038693 1101 44-45 0.47745350252254326 1101 46-47 0.9882232571534857 1101 48-49 0.9328145713589642 1101 50-51 0.6745018489244359 1101 52-53 1.0561614142114415 1101 54-55 1.0536152029412413 1101 56-57 0.6814466028516151 1101 58-59 1.115229728641669 1101 60-61 0.8437533932003625 1101 62-63 1.3741822670226043 1101 64-65 1.2746871197111567 1101 66-67 1.1552651162206118 1101 68-69 1.3136878195898802 1101 70-71 1.269956393192711 1101 72-73 1.5378137830089607 1101 74-75 0.9923677127175985 1101 76-77 1.333926160589133 1101 78-79 1.1124185225133267 1101 80-81 1.3066296625328455 1101 82-83 1.4980004231069408 1101 84-85 1.3208017860336305 1101 86-87 1.5815747101326423 1101 88-89 1.8596108802773124 1101 90-91 1.6605574258060116 1101 92-93 1.9158777466418364 1101 94-95 1.6991289486628496 1101 96-97 1.0343622252994003 1101 98-99 0.6914042285878885 1101 100-101 0.8829710805810471 1102 1 0.045276377827853764 1102 2 0.08881359736009387 1102 3 0.32926881733116176 1102 4 0.04969704859401958 1102 5 0.23788872255163795 1102 6 0.18596011355954545 1102 7 0.09786247695367933 1102 8 0.23506400733599975 1102 9 0.5240953124125127 1102 10-11 0.27134740396736845 1102 12-13 0.3577152689695424 1102 14-15 0.2839687292999642 1102 16-17 0.4919815376829533 1102 18-19 0.5036889383403818 1102 20-21 0.26308862385955933 1102 22-23 -0.2531266046376359 1102 24-25 0.10028012994401792 1102 26-27 0.22291557370701298 1102 28-29 0.5014887350154922 1102 30-31 0.6782883927692396 1102 32-33 0.49591237625847384 1102 34-35 0.6983360641806584 1102 36-37 0.8482163098722779 1102 38-39 0.6029423989776248 1102 40-41 0.5899791426675591 1102 42-43 0.8970136025957558 1102 44-45 0.5794445577687881 1102 46-47 1.1281773598747193 1102 48-49 1.0362594750659575 1102 50-51 0.9712238444129468 1102 52-53 0.9697584830443198 1102 54-55 0.15972006257640814 1102 56-57 -0.17079545313784905 1102 58-59 -0.6010677170182994 1102 60-61 -0.6174295048477028 1102 62-63 -0.34905192645006977 1102 64-65 0.02266933451563702 1102 66-67 -0.032190062218095505 1102 68-69 -0.2364340950568291 1102 70-71 -0.256975660580121 1102 72-73 -0.3758028443233883 1102 74-75 0.24825156130873438 1102 76-77 -0.32328505816616016 1102 78-79 0.21033489036958386 1102 80-81 0.42564965131930776 1102 82-83 0.23657499966542517 1102 84-85 0.5057208790408119 1102 86-87 0.5452741351127557 1102 88-89 0.10411168217579458 1102 90-91 0.4025025837734937 1102 92-93 0.44401590788210754 1102 94-95 0.7071022969475713 1102 96-97 0.41928001440662044 1102 98-99 0.1303882297353205 1102 100-101 0.41678120666817264 1103 1 0.26441634707054007 1103 2 0.4012468293430018 1103 3 0.5231337903891671 1103 4 0.35215176373670687 1103 5 0.2538191312012472 1103 6 0.2031349019562967 1103 7 -0.09698860835677436 1103 8 0.21511871308715058 1103 9 0.2619185870095535 1103 10-11 0.4640677824466479 1103 12-13 0.45316858711987607 1103 14-15 0.4606413048138549 1103 16-17 0.6675153883339036 1103 18-19 0.5065650990766812 1103 20-21 0.4056757597904337 1103 22-23 0.34546701145948333 1103 24-25 0.5285815515891841 1103 26-27 0.5882995118167287 1103 28-29 0.2604018984401222 1103 30-31 0.34566920819015223 1103 32-33 0.6372081747159086 1103 34-35 0.4681375503926901 1103 36-37 0.591204934363077 1103 38-39 0.9878490117994332 1103 40-41 0.5988717620869295 1103 42-43 0.7449644848140409 1103 44-45 0.5726650360332286 1103 46-47 0.6435971032507481 1103 48-49 0.4641676257533014 1103 50-51 -0.5375220321117169 1103 52-53 0.0017425643751032283 1103 54-55 0.33374539626975164 1103 56-57 0.27792084001923456 1103 58-59 0.15582626417513268 1103 60-61 0.6426341228714456 1103 62-63 0.654287941833509 1103 64-65 0.4663728903894615 1103 66-67 0.4530241237110211 1103 68-69 0.04969694047843376 1103 70-71 0.19585530334460444 1103 72-73 0.02989430182079289 1103 74-75 -0.03159300861718961 1103 76-77 0.3896506214882258 1103 78-79 0.5443980250326028 1103 80-81 0.8156218288664725 1103 82-83 0.41757355571534305 1103 84-85 0.3086099531518478 1103 86-87 0.4281439565560028 1103 88-89 0.47666841297888496 1103 90-91 0.1785846658790753 1103 92-93 0.18965764390924633 1103 94-95 0.5974383968736099 1103 96-97 1.0927653342474315 1103 98-99 0.9458267562018676 1103 100-101 1.0471593337592573 1104 1 0.19632727833771213 1104 2 0.27917392357330684 1104 3 0.5361354342751738 1104 4 0.4349783280149495 1104 5 0.08655660375524832 1104 6 0.40068861564422775 1104 7 0.31279883603274783 1104 8 0.21891229618386632 1104 9 0.3927052381953331 1104 10-11 0.6162249517439093 1104 12-13 0.46755546362150113 1104 14-15 0.5511872359085928 1104 16-17 0.16307229782820798 1104 18-19 0.26535644051065077 1104 20-21 0.4763743538655518 1104 22-23 0.5769503024502924 1104 24-25 0.6630468288081346 1104 26-27 0.6029392938581282 1104 28-29 0.16000414700028642 1104 30-31 0.265233750833481 1104 32-33 0.10032994873331802 1104 34-35 0.20261547288864534 1104 36-37 0.7907071702779618 1104 38-39 0.6096977512952364 1104 40-41 0.520402070516738 1104 42-43 0.7260023171836494 1104 44-45 0.34179676533233305 1104 46-47 0.5458721035381373 1104 48-49 0.28804765656179754 1104 50-51 -0.00816774593759817 1104 52-53 0.3003550326182136 1104 54-55 0.2743470815403022 1104 56-57 0.5628419219951191 1104 58-59 0.4765852107086026 1104 60-61 0.59281173153461 1104 62-63 0.3222344638075505 1104 64-65 0.726308054605628 1104 66-67 0.6403006759187697 1104 68-69 0.6708616854461873 1104 70-71 0.9440648470800177 1104 72-73 0.8255983563564264 1104 74-75 0.6960794557173813 1104 76-77 0.7362231766388803 1104 78-79 0.7467282047104966 1104 80-81 0.7980621374112324 1104 82-83 0.28095858140824603 1104 84-85 0.34501685517394165 1104 86-87 0.4192577755446081 1104 88-89 0.5483326463877098 1104 90-91 0.1396429606059968 1104 92-93 -0.06874284350870496 1104 94-95 0.06810710211220439 1104 96-97 0.16809095012990127 1104 98-99 0.12340668010029177 1104 100-101 0.17657799588895529 1105 1 -0.011248104756699462 1105 2 0.17450264329648846 1105 3 0.23858229537255582 1105 4 -0.015207040249997306 1105 5 0.16062976243048155 1105 6 0.09300201159084764 1105 7 0.042297105928938095 1105 8 -0.23338627030155124 1105 9 0.31690197571387557 1105 10-11 0.44783024091840673 1105 12-13 0.03406559708665213 1105 14-15 0.008564892853719641 1105 16-17 0.3322579132508494 1105 18-19 0.1990069595417907 1105 20-21 0.047660809624325395 1105 22-23 -0.3663270084740802 1105 24-25 -0.06157625571978542 1105 26-27 0.09822476098615596 1105 28-29 0.23843345990191267 1105 30-31 0.08341837762537097 1105 32-33 0.3027397361777062 1105 34-35 0.36398472647243096 1105 36-37 0.14843084798433637 1105 38-39 0.3449918689422944 1105 40-41 -0.14107840402602534 1105 42-43 0.13080335524593778 1105 44-45 0.36548065065116475 1105 46-47 -0.3963115346230026 1105 48-49 -0.2954669257417173 1105 50-51 -0.14030442413829292 1105 52-53 -0.22317437914649574 1105 54-55 -0.01014995256745621 1105 56-57 0.06666668719531543 1105 58-59 -0.08935646007735798 1105 60-61 -0.13128614290928553 1105 62-63 -0.4088765099937355 1105 64-65 -0.7645656478165179 1105 66-67 -0.7524576038637321 1105 68-69 -0.04752066749498951 1105 70-71 -0.6651912082833036 1105 72-73 -0.2743831068170799 1105 74-75 -0.0748239056271558 1105 76-77 0.16386158494337977 1105 78-79 0.09601762636150823 1105 80-81 -0.11386322096741708 1105 82-83 -0.24347295591256568 1105 84-85 -0.4229515086421749 1105 86-87 -0.41915670789582293 1105 88-89 0.18825479171642812 1105 90-91 0.42297835358671776 1105 92-93 0.7711360492248609 1105 94-95 0.2324051742822526 1105 96-97 0.4971257993637117 1105 98-99 0.34113406517196765 1105 100-101 0.4755646493738759 1106 1 -0.08267667618527241 1106 2 0.09235978615363294 1106 3 -0.23998913319887194 1106 4 -0.015207040249997306 1106 5 0.05348690528762745 1106 6 0.20371629730512808 1106 7 0.2637256773575132 1106 8 0.2773280154127349 1106 9 0.02761626142815743 1106 10-11 0.1514016694898288 1106 12-13 0.2644227399437966 1106 14-15 0.5192791785680058 1106 16-17 0.11975791325085083 1106 18-19 0.1454355309703672 1106 20-21 0.1905179524814642 1106 22-23 0.29617299152592125 1106 24-25 0.24913802999449786 1106 26-27 0.28572476098615596 1106 28-29 0.1884334599019155 1106 30-31 0.2584183776253681 1106 32-33 0.4241683076062799 1106 34-35 0.3246990121867199 1106 36-37 0.07700227655576697 1106 38-39 0.1235632975137193 1106 40-41 0.1392787388311163 1106 42-43 -0.3049109304683526 1106 44-45 -0.004162206491692189 1106 46-47 0.06797417966270558 1106 48-49 0.2741759314011425 1106 50-51 0.4936241472902765 1106 52-53 0.591111335139221 1106 54-55 0.7594929045753993 1106 56-57 0.38988097290960155 1106 58-59 0.32492925420835306 1106 60-61 0.70085671423357 1106 62-63 0.6732663471491236 1106 64-65 0.5265057807549098 1106 66-67 0.4171852532791256 1106 68-69 0.7131936182192931 1106 70-71 1.1080230774309818 1106 72-73 1.17025975032578 1106 74-75 0.5394618086585581 1106 76-77 0.006718727800521407 1106 78-79 0.23530334064722425 1106 80-81 0.02363677903258221 1106 82-83 0.17974132980172186 1106 84-85 0.29847706278639663 1106 86-87 0.5987004349613194 1106 88-89 0.22754050600213915 1106 90-91 0.7694069250152928 1106 92-93 0.4318503349391456 1106 94-95 0.6538337457108234 1106 96-97 1.2578400850779978 1106 98-99 1.0679197794576787 1106 100-101 1.071993220802451 1107 1 0.22089475238615464 1107 2 0.2467248655187113 1107 3 0.25630716309742496 1107 4 0.25926385922090134 1107 5 0.17835463015535424 1107 6 -0.004617036028200516 1107 7 0.29877858741042473 1107 8 0.19783066091537904 1107 9 0.41028821910011715 1107 10-11 0.4385048440930035 1107 12-13 0.5890920521131093 1107 14-15 0.8790675383563666 1107 16-17 1.1884087069016402 1107 18-19 1.0277106632455002 1107 20-21 0.9270920265555418 1107 22-23 0.906688864541799 1107 24-25 0.6531724215288932 1107 26-27 0.6885025387639416 1107 28-29 0.45516626413471784 1107 30-31 0.5772675839745816 1107 32-33 0.7642476726856415 1107 34-35 0.5970535095412117 1107 36-37 0.8078223823758748 1107 38-39 0.3449918689422944 1107 40-41 0.2553501674025398 1107 42-43 -0.012252200309625039 1107 44-45 -0.8061463334758159 1107 46-47 -0.3898961907076668 1107 48-49 0.2325754023006148 1107 50-51 -0.14724886858273578 1107 52-53 -0.2324336384057517 1107 54-55 -0.13018963510713988 1107 56-57 0.05125663428526295 1107 58-59 -0.03671095743185404 1107 60-61 -0.15562476724791097 1107 62-63 -0.27838709200431566 1107 64-65 -0.47243601818688674 1107 66-67 -0.3924046938108212 1107 68-69 -0.3424280749024007 1107 70-71 -0.21056157865367098 1107 72-73 -0.08053390046787356 1107 74-75 -0.09433448763773811 1107 76-77 0.18932454790634168 1107 78-79 0.1058721237160043 1107 80-81 0.18018439808020403 1107 82-83 0.49290270546309856 1107 84-85 0.7744029887123212 1107 86-87 1.1773380010988852 1107 88-89 0.8137177546793914 1107 90-91 0.8877270308353964 1107 92-93 0.8665064195952326 1107 94-95 1.1343893012663777 1107 96-97 0.6425623073002207 1107 98-99 1.1237398852777893 1107 100-101 1.9025487763580067 1108 1 0.3851804666718728 1108 2 0.3066455004393447 1108 3 0.45643943822969746 1108 4 0.5633643883214248 1108 5 0.36420119100191073 1108 6 0.5251448687337046 1108 7 0.6708685345003715 1108 8 0.634470872555589 1108 9 0.15618768999959087 1108 10-11 0.32640166948982596 1108 12-13 0.6644227399437952 1108 14-15 0.7460648928537168 1108 16-17 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2316 80-81 -2.148584619900433 2316 82-83 -2.133914568242144 2316 84-85 -1.6473735596202417 2316 86-87 -1.69360098768243 2316 88-89 -1.736797075503489 2316 90-91 -1.2478500400113823 2316 92-93 -1.5007519175801143 2316 94-95 -1.580502353874241 2316 96-97 -2.192108047702085 2316 98-99 -2.4811317913781217 2316 100-101 -2.990143708658131 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 431.0 3 144.0 4 134.0 5 136.0 6 144.0 7 233.0 8 339.0 9 405.0 10 665.0 11 1122.0 12 1838.0 13 2821.0 14 3438.0 15 4738.0 16 6632.0 17 8859.0 18 7119.0 19 3342.0 20 3214.0 21 3249.0 22 3794.0 23 4173.0 24 4427.0 25 4428.0 26 4905.0 27 5463.0 28 6283.0 29 7034.0 30 8146.0 31 9799.0 32 11916.0 33 14867.0 34 19470.0 35 28308.0 36 32522.0 37 32006.0 38 21497.0 39 3455.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.1078783145579 17.873378178254878 13.899224177945435 14.119519329241786 2 15.133331123119936 24.822906651637215 38.070925747880956 21.97283647736189 3 24.225423710083213 33.44703485823532 29.468341001432936 12.859200430248535 4 10.899771915796766 17.494316318521985 38.91212309914477 32.69378866653647 5 10.110107159176334 43.35708916174108 29.83469186209336 16.698111816989226 6 23.244715351278046 37.786343937026444 18.092735634894897 20.876205076800613 7 23.00284058462253 41.88185968027765 18.736068792991016 16.379230942108812 8 26.593843830014553 27.07314826754103 19.046917309853185 27.28609059259123 9 29.722381857565978 19.189027894434375 21.80422321441728 29.284367033582363 10-11 24.71557148252895 28.060542970585256 22.32554283240136 24.898342714484436 12-13 33.016491848041376 27.55691815614101 23.473476885434074 15.953113110383544 14-15 27.638438102115025 25.8897947605171 31.41976650370801 15.052000633659866 16-17 17.349369182506948 31.034673317773965 30.661837982415584 20.9541195173035 18-19 16.51256316585516 29.407516713735937 37.38810685737551 16.6918132630334 20-21 21.226752240037726 24.42470083706673 35.39996463098326 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18.460818782379366 30.760234779742053 31.81479141842104 18.964155019457547 48-49 18.525421325293884 30.353101075712402 31.232124407676764 19.889353191316953 50-51 17.458092842172725 31.942444188247947 32.374499526691295 18.224963442888033 52-53 17.439491994268728 33.50620457986025 29.25637125082415 19.79793217504687 54-55 15.57717312431166 31.311628181091542 30.920265058732095 22.190933635864702 56-57 19.046285996331303 31.748414282872783 27.637152560353016 21.568147160442898 58-59 20.08442330996836 30.47453119647573 28.460453278033366 20.980592215522545 60-61 19.152505851868085 30.262859836939604 31.36472377893475 19.219910532257558 62-63 20.57180111457754 30.618468984467373 30.154184454111153 18.65554544684393 64-65 19.455776048621022 32.9512408490262 30.302684285648514 17.29029881670427 66-67 19.211346357850072 31.08314179632249 29.301479255068365 20.404032590759076 68-69 18.2682992415452 32.14850095589617 29.927101406033014 19.65609839652562 70-71 19.990864434469817 31.592111733860346 28.130358626202494 20.28666520546734 72-73 18.704233030307496 29.694199289829236 30.624088344064475 20.977479335798797 74-75 17.421188240255926 33.31025045536502 29.71816174344486 19.550399560934192 76-77 17.926707086184923 30.192532948291994 30.60323552595714 21.277524439565944 78-79 17.231354255330906 30.092597710297763 32.29570837432615 20.380339660045173 80-81 17.914420770684654 31.645856437047982 30.30034522015614 20.139377572111222 82-83 16.653375447772877 30.281878205777534 31.20219535329165 21.86255099315794 84-85 17.073309991325026 29.98373672277467 30.298688071079543 22.644265214820763 86-87 17.319281546531503 30.731346142511935 30.298704838804742 21.650667472151824 88-89 17.717338853751883 28.83503943243811 29.741930255601762 23.705691458208246 90-91 17.08364261940591 28.019199988211824 31.23368765391395 23.66346973846831 92-93 17.463565765818867 30.45316229996169 31.05069847631959 21.032573457899854 94-95 16.6168762040881 30.0219912992548 30.671600281429694 22.68953221522741 96-97 14.550135920613522 29.560339175341277 31.763616004007634 24.125908900037572 98-99 16.713272543059777 29.918393663074518 29.173804918485768 24.194528875379937 100-101 16.90085568355186 28.394174571236945 30.945932002270034 23.759037742941164 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 771.0 1 635.0 2 858.5 3 1182.0 4 1021.0 5 984.5 6 1440.5 7 2614.0 8 3888.5 9 4289.5 10 3456.0 11 2509.0 12 2281.0 13 2202.0 14 2121.5 15 1948.0 16 1739.5 17 1642.0 18 1566.5 19 1523.0 20 1509.0 21 1516.5 22 1565.0 23 1670.5 24 1783.0 25 1901.5 26 2137.0 27 2425.0 28 2726.0 29 2936.5 30 3284.0 31 3661.5 32 4122.5 33 4651.0 34 5080.0 35 5411.0 36 5939.0 37 6636.0 38 6548.5 39 6148.0 40 5764.0 41 5249.5 42 5063.5 43 4984.5 44 5034.5 45 5186.0 46 6127.5 47 11823.5 48 28767.5 49 28021.5 50 11870.0 51 6623.0 52 3749.5 53 2792.5 54 2550.0 55 2393.0 56 2500.5 57 2641.5 58 2621.0 59 2810.5 60 3173.5 61 3503.5 62 3079.0 63 1660.0 64 751.0 65 597.5 66 474.5 67 346.5 68 252.5 69 187.5 70 156.5 71 141.5 72 124.0 73 146.5 74 117.5 75 71.5 76 55.5 77 35.0 78 28.5 79 22.0 80 15.5 81 9.5 82 8.0 83 5.0 84 3.5 85 5.0 86 3.5 87 2.5 88 3.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015469841176297256 2 0.01068155700268144 3 0.009208238795415033 4 0.03867460294074314 5 0.045672864425258565 6 0.05303945546159059 7 0.0268880572826119 8 0.022468102660812683 9 0.01620650027993046 10-11 0.04364705189026726 12-13 0.04401538144208386 14-15 0.020994784453546276 16-17 0.023020596988537586 18-19 0.032228835783952615 20-21 0.026519727730795296 22-23 0.013259863865397648 24-25 0.0020258125349913074 26-27 0.0020258125349913074 28-29 0.0011049886554498041 30-31 0.007182426260423726 32-33 0.0014733182072664054 34-35 0.002394142086807909 36-37 0.007734920588148628 38-39 0.009024074019506733 40-41 0.00534077850134072 42-43 9.208238795415034E-4 44-45 0.0012891534313581045 46-47 0.00276247163862451 48-49 5.524943277249021E-4 50-51 0.0011049886554498041 52-53 0.0011049886554498041 54-55 0.003314965966349412 56-57 0.0036832955181660136 58-59 0.002578306862716209 60-61 5.524943277249021E-4 62-63 0.0011049886554498041 64-65 0.0031308011904411116 66-67 0.0029466364145328107 68-69 0.008471579691781831 70-71 0.01086572177858974 72-73 0.0044199546217992165 74-75 0.003867460294074314 76-77 0.0036832955181660136 78-79 0.039411262044376345 80-81 0.027624716386245102 82-83 0.004972448949524118 84-85 0.009392403571323335 86-87 0.008839909243598433 88-89 0.006998261484515425 90-91 0.013812358193122551 92-93 0.017679818487196866 94-95 0.009208238795415033 96-97 0.005156613725432418 98-99 0.026151398178978696 100-101 0.049356159943424585 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 271496.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.17914119838071 #Duplication Level Percentage of deduplicated Percentage of total 1 79.77995019510618 29.661500331036333 2 10.602993380781033 7.884203760591081 3 3.5760594030539194 3.9886445243981603 4 1.7539996857033089 2.6084880790671483 5 0.9959993896655343 1.8515200970937955 6 0.624999610011723 1.3942169249735237 7 0.4209997371822127 1.0956686071225061 8 0.3159998027282343 0.939888102743478 9 0.25999983768773766 0.8699913609253618 >10 1.370999144114948 9.578456167316233 >50 0.1369999144739226 3.6654892424628627 >100 0.11399992883231516 8.314366155715286 >500 0.021999986265885382 5.544521865589682 >1k 0.021999986265885382 15.82454798934444 >5k 0.0029999981271661884 6.778496791620135 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7251 2.6707575802221766 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5554 2.0457023307894038 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5427 1.9989244777086954 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3798 1.3989156377994518 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3666 1.3502961369596607 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3593 1.3234080796770487 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3056 1.1256151103515337 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2868 1.0563691546100127 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2811 1.0353743701564664 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2421 0.8917258449479919 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2206 0.8125349913074226 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1609 0.5926422488729116 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1510 0.5561776232430681 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1447 0.5329728614786222 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1405 0.5175030203023249 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1390 0.5119780770250759 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1351 0.49761322450422846 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1343 0.49466658808969566 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1333 0.4909832925715296 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1281 0.4718301558770664 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1188 0.43757550755812236 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1128 0.4154757344491263 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1077 0.39669092730647965 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1067 0.39300763178831366 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1015 0.3738544950938504 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 908 0.334443233049474 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 900 0.3314965966349412 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 848 0.3123434599404779 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 824 0.3035035506968795 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 777 0.28619206176149925 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 753 0.2773521525179008 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 709 0.26114565223797037 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 706 0.26004066358252054 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 698 0.25709402716798774 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 668 0.24604414061348967 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 667 0.24567581106167308 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 640 0.23573091316262487 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 633 0.23315260629990864 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 631 0.23241594719627545 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 607 0.223576037952677 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 604 0.22247104929722725 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 593 0.2184194242272446 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 574 0.2114211627427292 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 567 0.20884285588001297 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 552 0.20331791260276394 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 545 0.20073960574004773 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 509 0.18747974187465008 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 495 0.18232312814921767 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 488 0.17974482128650146 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 484 0.17827150307923506 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 426 0.15690838907387217 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 404 0.14880513893390696 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 388 0.14291186610484133 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 382 0.14070188879394172 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 361 0.1329669682057931 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 360 0.1325986386539765 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 343 0.12633703627309428 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 342 0.12596870672127766 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 336 0.12375872941037805 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 321 0.11823378613312903 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 306 0.11270884285588001 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 291 0.10718389957863099 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 290 0.1068155700268144 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 282 0.10386893361228157 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 282 0.10386893361228157 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 280 0.10313227450864837 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 277 0.10202728585319858 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.8416477590830067E-4 0.0 22-23 0.0 0.0 0.0 0.0016574829831747062 0.0 24-25 0.0 0.0 0.0 0.0025783068627162095 0.0 26-27 0.0 0.0 0.0 0.004788284173615817 0.0 28-29 0.0 0.0 0.0 0.0058932728290656215 0.0 30-31 0.0 0.0 0.0 0.009024074019506733 0.0 32-33 0.0 0.0 0.0 0.014917346848572353 0.0 34-35 0.0 0.0 0.0 0.01933730147037157 0.0 36-37 0.0 0.0 0.0 0.023388926540354186 0.0 38-39 0.0 0.0 0.0 0.028729705041694906 0.0 40-41 0.0 0.0 0.0 0.040516250699826145 0.0 42-43 0.0 0.0 0.0 0.052855290685682295 0.0 44-45 0.0 0.0 0.0 0.0801116775201108 0.0 46-47 0.0 0.0 0.0 0.14696349117482393 0.0 48-49 0.0 0.0 0.0 0.20092377051595603 0.0 50-51 0.0 0.0 0.0 0.25248990777028024 0.0 52-53 0.0 0.0 0.0 0.30460853935232934 0.0 54-55 0.0 0.0 0.0 0.3631729380911689 0.0 56-57 0.0 0.0 0.0 0.42578896189999116 0.0 58-59 0.0 0.0 0.0 0.5453119014644783 0.0 60-61 0.0 0.0 0.0 0.6996419836756342 0.0 62-63 0.0 0.0 0.0 0.8585761852844978 0.0 64-65 0.0 0.0 0.0 0.9613401302413296 0.0 66-67 0.0 0.0 0.0 1.139243303768748 0.0 68-69 0.0 0.0 0.0 1.353058608598285 0.0 70-71 0.0 0.0 0.0 1.4887880484427027 0.0 72-73 0.0 0.0 0.0 1.7320697174175677 0.0 74-75 0.0 0.0 0.0 1.9162344933258684 0.0 76-77 0.0 0.0 0.0 2.0560155582402686 0.0 78-79 0.0 0.0 0.0 2.2597018003948492 0.0 80-81 0.0 0.0 0.0 2.434842502283643 0.0 82-83 0.0 0.0 0.0 2.672783392757168 0.0 84-85 0.0 0.0 0.0 3.0438754162123933 0.0 86-87 0.0 0.0 0.0 3.5930547779709463 0.0 88-89 0.0 0.0 0.0 4.176304623272534 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATAGTC 25 5.259514E-5 76.024925 7 GCGTATC 25 0.005003336 57.03972 1 GAGCCGA 25 0.0050106454 57.018692 9 GACCTAT 25 0.0050106454 57.018692 9 AGTGTGC 25 0.0050106454 57.018692 8 AGATAGT 35 2.7745747E-4 54.32354 6 GTTCGAC 20 5.376952E-4 47.515575 16-17 ATAGTCA 40 5.3721684E-4 47.515575 8 CGACCGT 20 5.379396E-4 47.511196 18-19 TCCGCCA 20 5.386733E-4 47.498066 36-37 CCGCCAG 20 5.386733E-4 47.498066 38-39 CGATCCG 20 5.3916295E-4 47.489315 70-71 CGTCACT 55 4.9329214E-5 43.21191 1 AGGAGTG 45 9.57915E-4 42.251644 5 TAGATAG 45 9.57915E-4 42.251644 5 TATGTAC 45 9.57915E-4 42.251644 4 CGACTTA 25 0.0016124647 38.012463 12-13 TCGACCG 25 0.0016131949 38.008957 18-19 GACCGTC 25 0.0016139256 38.005455 20-21 CGTGGGC 25 0.0016168506 37.99145 48-49 >>END_MODULE