##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936698_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 271496 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.451413648819873 31.0 31.0 34.0 30.0 34.0 2 31.531201196334383 31.0 31.0 34.0 30.0 34.0 3 31.742924389309604 31.0 31.0 34.0 30.0 34.0 4 35.59771414680143 37.0 35.0 37.0 33.0 37.0 5 35.47106034711377 37.0 35.0 37.0 33.0 37.0 6 35.71062557091081 37.0 35.0 37.0 33.0 37.0 7 35.64128384948581 37.0 35.0 37.0 33.0 37.0 8 35.686547868108555 37.0 35.0 37.0 33.0 37.0 9 37.19121460353007 39.0 37.0 39.0 33.0 39.0 10-11 37.1015097828329 39.0 37.0 39.0 33.0 39.0 12-13 36.95774155052008 39.0 37.0 39.0 32.5 39.0 14-15 37.63684732003418 39.0 36.5 41.0 32.0 41.0 16-17 37.692321433833285 39.0 36.5 41.0 32.5 41.0 18-19 38.07130123464066 39.5 37.0 41.0 33.5 41.0 20-21 38.250705351091725 40.0 37.5 41.0 34.0 41.0 22-23 38.145333632908034 40.0 37.0 41.0 33.5 41.0 24-25 37.94995138049916 40.0 36.5 41.0 33.0 41.0 26-27 37.542676503521236 39.5 36.0 41.0 32.5 41.0 28-29 37.30600082505819 39.0 35.0 41.0 32.5 41.0 30-31 36.94658116510004 38.5 35.0 40.0 32.5 41.0 32-33 36.35077864867254 38.0 35.0 40.0 31.5 41.0 34-35 35.9342955328992 38.0 35.0 40.0 31.0 41.0 36-37 35.440634484485955 37.5 35.0 40.0 30.0 41.0 38-39 35.05806531219613 37.0 35.0 40.0 29.5 41.0 40-41 34.59851894687215 37.0 34.0 40.0 26.0 41.0 42-43 34.00256173203289 37.0 33.5 40.0 23.0 41.0 44-45 33.16344071367534 37.0 33.0 40.0 16.0 41.0 46-47 31.971879880366565 36.0 32.5 40.0 2.0 41.0 48-49 30.914831157733445 36.0 31.0 40.0 2.0 41.0 50-51 30.496510077496538 35.0 30.0 40.0 2.0 41.0 52-53 30.16964522497569 35.0 29.0 39.5 2.0 41.0 54-55 29.91024177151781 35.0 28.0 39.0 2.0 41.0 56-57 29.59067721071397 35.0 28.0 39.0 2.0 40.5 58-59 29.276096148746205 35.0 27.0 39.0 2.0 40.0 60-61 28.75895593305242 35.0 26.0 38.5 2.0 40.0 62-63 28.412125777175355 35.0 26.0 38.0 2.0 40.0 64-65 27.88902230603766 34.5 24.5 37.5 2.0 40.0 66-67 27.36555971358694 34.0 22.5 37.0 2.0 39.0 68-69 26.891698220231603 34.0 22.0 36.0 2.0 39.0 70-71 26.300483248371982 33.0 20.0 35.5 2.0 38.5 72-73 25.839826369449273 33.0 18.5 35.0 2.0 37.5 74-75 25.518946872145445 33.0 15.0 35.0 2.0 37.0 76-77 24.087178079971714 30.5 7.5 34.0 2.0 35.5 78-79 24.727902068538764 32.0 4.5 35.0 2.0 36.0 80-81 24.46256298435336 32.0 2.0 35.0 2.0 36.0 82-83 24.07005075581224 32.0 2.0 35.0 2.0 35.0 84-85 23.596371585585054 32.0 2.0 35.0 2.0 35.0 86-87 23.20395143943189 31.0 2.0 35.0 2.0 35.0 88-89 22.632847261101453 31.0 2.0 34.0 2.0 35.0 90-91 21.993519241535786 30.5 2.0 34.0 2.0 35.0 92-93 21.54410562218228 30.0 2.0 34.0 2.0 35.0 94-95 21.011576597813594 29.5 2.0 34.0 2.0 35.0 96-97 20.296680245749478 29.0 2.0 34.0 2.0 35.0 98-99 19.566791039278662 29.0 2.0 34.0 2.0 35.0 100-101 17.441686065356397 22.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3200391563464322 1101 2 0.37965200704267943 1101 3 -0.0498391759646708 1101 4 -0.038714772308473755 1101 5 0.16805360261820113 1101 6 0.14385980864032177 1101 7 0.4274941089018398 1101 8 0.36195959245557674 1101 9 0.302829332011342 1101 10-11 0.07639753626239809 1101 12-13 0.20920655959675827 1101 14-15 0.2916004928222904 1101 16-17 0.15918773453837787 1101 18-19 0.35057788937593415 1101 20-21 0.2617550789093599 1101 22-23 0.023304165420988454 1101 24-25 -0.027814608938385277 1101 26-27 0.019935621958190097 1101 28-29 -0.06751541466110211 1101 30-31 0.2663126419855786 1101 32-33 0.5498710725011691 1101 34-35 0.7495612050912825 1101 36-37 0.5459326403925289 1101 38-39 0.5294377111364241 1101 40-41 0.8049169976502384 1101 42-43 1.0348539502356786 1101 44-45 1.018872948547326 1101 46-47 1.446608640900056 1101 48-49 0.9629853280216061 1101 50-51 0.9761528409238132 1101 52-53 0.8617555363560889 1101 54-55 0.9230935101535813 1101 56-57 0.9916525919140184 1101 58-59 0.7423923946649147 1101 60-61 0.810169363815195 1101 62-63 0.6539694847657103 1101 64-65 0.5006150544655483 1101 66-67 0.06694487250727832 1101 68-69 0.17652680574080648 1101 70-71 0.5718249742998687 1101 72-73 0.3714482754131545 1101 74-75 0.5584509436036846 1101 76-77 0.7220572462169947 1101 78-79 0.6847227934794624 1101 80-81 0.7975085994189044 1101 82-83 1.1663972745879967 1101 84-85 1.4833210019204905 1101 86-87 1.1367360210491455 1101 88-89 0.9232798766562524 1101 90-91 0.7901072564498435 1101 92-93 1.3007220106875579 1101 94-95 1.0176605972929238 1101 96-97 1.084511203581279 1101 98-99 1.1062582893130255 1101 100-101 0.6552413827123758 1102 1 0.5290282427037276 1102 2 0.5353507018388157 1102 3 0.29914964961263024 1102 4 0.1387590518997328 1102 5 0.03882407710739244 1102 6 0.3117238901079915 1102 7 0.19083626529160114 1102 8 0.34869892981361517 1102 9 0.33055024526287724 1102 10-11 0.5557372413202231 1102 12-13 0.32752896190205405 1102 14-15 0.5845607369123584 1102 16-17 0.412561575697282 1102 18-19 0.42575424184617106 1102 20-21 0.33494298393328137 1102 22-23 0.10288118027241211 1102 24-25 0.3865061243749537 1102 26-27 0.046528661740183 1102 28-29 0.2183516136204915 1102 30-31 0.13254554459729206 1102 32-33 0.2786012043254473 1102 34-35 0.6052716349871048 1102 36-37 0.5606275929957647 1102 38-39 0.5319346795690052 1102 40-41 0.8647399280350214 1102 42-43 0.7532767528382678 1102 44-45 1.0453225593331865 1102 46-47 0.9284127168913479 1102 48-49 1.265235859360839 1102 50-51 1.1987546429135598 1102 52-53 0.7928835402156373 1102 54-55 1.1998136801821353 1102 56-57 1.2472104655139518 1102 58-59 0.9692449105398993 1102 60-61 1.1009064584652926 1102 62-63 1.0353602244681603 1102 64-65 0.9587794240951091 1102 66-67 1.0153691093998276 1102 68-69 1.1522938248950965 1102 70-71 0.7127374434952323 1102 72-73 1.1545053992706116 1102 74-75 0.5755711501414389 1102 76-77 0.9358772819438528 1102 78-79 1.3267435776449013 1102 80-81 1.3196748988204163 1102 82-83 0.872935029011483 1102 84-85 1.0158272280428235 1102 86-87 1.0272345020056832 1102 88-89 1.1716467419503438 1102 90-91 1.0943788327345132 1102 92-93 1.3433204225373991 1102 94-95 1.327271643933429 1102 96-97 1.9652499933013274 1102 98-99 1.6798178058269144 1102 100-101 1.422149705505852 1103 1 0.4923307795948908 1103 2 0.5811697032827681 1103 3 0.46647176787566025 1103 4 -0.00860603268287008 1103 5 0.24137622953217885 1103 6 0.10985262471466228 1103 7 0.30644618745917285 1103 8 0.1962389319267146 1103 9 0.20519659488662967 1103 10-11 0.24793521509433702 1103 12-13 0.5147318075402723 1103 14-15 0.19448877419679178 1103 16-17 0.01553986948830044 1103 18-19 0.2307681530725887 1103 20-21 0.04673993872473403 1103 22-23 0.17380495614318647 1103 24-25 0.2259459187001127 1103 26-27 0.10320663739259572 1103 28-29 0.17418200231306713 1103 30-31 0.7508966240380346 1103 32-33 0.46956066806821894 1103 34-35 0.470209474696901 1103 36-37 0.6452571552323647 1103 38-39 0.7786623130170014 1103 40-41 1.0319094336875594 1103 42-43 1.085631800206876 1103 44-45 1.2795476981519656 1103 46-47 1.5176265271586509 1103 48-49 1.7805675570000439 1103 50-51 1.4495441196871006 1103 52-53 1.1101710271555199 1103 54-55 1.0893045410346538 1103 56-57 1.0283874609238346 1103 58-59 1.1626520457664107 1103 60-61 1.3249417706754762 1103 62-63 0.9740158247639918 1103 64-65 0.6788585478639355 1103 66-67 0.9997028379606903 1103 68-69 1.095480845779548 1103 70-71 0.530946475813888 1103 72-73 1.1538832093970477 1103 74-75 1.1618148842198366 1103 76-77 0.9530462006248115 1103 78-79 1.2810008825061985 1103 80-81 1.520706794856011 1103 82-83 1.81465462094884 1103 84-85 2.1577334769422514 1103 86-87 2.0620771349253992 1103 88-89 1.688128903884067 1103 90-91 1.0310445993780917 1103 92-93 1.0104840584741481 1103 94-95 1.1008825756921112 1103 96-97 1.045882279086051 1103 98-99 1.0092262558697627 1103 100-101 0.9038714105408943 1104 1 0.32251620533837766 1104 2 0.2952025120286983 1104 3 0.08388316471894441 1104 4 -0.011594916334829009 1104 5 0.24639985320873592 1104 6 0.18281587294144686 1104 7 0.0072129510729581625 1104 8 -0.0640928341586573 1104 9 -0.12358060682852567 1104 10-11 -0.04131974817688189 1104 12-13 -0.007133025944426663 1104 14-15 0.256519605681234 1104 16-17 0.40333027977006 1104 18-19 0.21273138980366468 1104 20-21 0.1356937144894843 1104 22-23 0.32185241041901236 1104 24-25 0.5314558107554319 1104 26-27 0.469896678662181 1104 28-29 -0.028240143015651142 1104 30-31 -0.028788163693747038 1104 32-33 0.12978782322576166 1104 34-35 0.044540711979777825 1104 36-37 -0.49303619220237493 1104 38-39 -0.4677596483804223 1104 40-41 -0.7871424004523888 1104 42-43 -0.6867014833117366 1104 44-45 -0.23379881692465432 1104 46-47 0.17334161757237965 1104 48-49 -0.1527401025891031 1104 50-51 -0.2505099101327666 1104 52-53 0.29692452472417585 1104 54-55 0.6504904381020964 1104 56-57 0.7225671847969686 1104 58-59 0.7589975607165762 1104 60-61 0.45434893709881763 1104 62-63 -0.16471435951217828 1104 64-65 0.38293607490758674 1104 66-67 0.4920524453823134 1104 68-69 0.1435271504847364 1104 70-71 0.3947223555228163 1104 72-73 0.490506230698589 1104 74-75 0.47287145459080193 1104 76-77 0.8224607243002175 1104 78-79 1.159767163300323 1104 80-81 0.7262615201661049 1104 82-83 0.6029899277117643 1104 84-85 0.5310680364842639 1104 86-87 0.5900806756029553 1104 88-89 0.46975646598961873 1104 90-91 0.338250108120576 1104 92-93 0.2577567021417373 1104 94-95 -0.04313563350675409 1104 96-97 -0.44447282328321336 1104 98-99 -0.17237141739926898 1104 100-101 0.012689766884800946 1105 1 0.21060320484406247 1105 2 0.40035574979439303 1105 3 0.12452824628097758 1105 4 0.11099529622078563 1105 5 -0.23437127877347308 1105 6 0.08360677753858425 1105 7 0.060599011379437684 1105 8 -0.011485320564979418 1105 9 0.01491420286005507 1105 10-11 0.15403986625712918 1105 12-13 0.04309559491568393 1105 14-15 0.438674820708421 1105 16-17 0.03161129805972962 1105 18-19 0.17943924941810963 1105 20-21 0.09491269619982035 1105 22-23 0.10059067042890035 1105 24-25 0.03134458979645416 1105 26-27 0.1905405244357894 1105 28-29 -0.07581799768693287 1105 30-31 0.11223383334035475 1105 32-33 0.055399538500104484 1105 34-35 0.19554170061052645 1105 36-37 0.22403622499980003 1105 38-39 0.37463407381434166 1105 40-41 -0.06937794172772982 1105 42-43 0.12944409256567013 1105 44-45 -0.12580944470517608 1105 46-47 -0.2212023764958353 1105 48-49 -0.306558688847133 1105 50-51 -0.20867847167502873 1105 52-53 0.3595481035674766 1105 54-55 0.09848228189843766 1105 56-57 0.44296387288396843 1105 58-59 0.5447118464308609 1105 60-61 0.04707632217049351 1105 62-63 0.34129157227230067 1105 64-65 0.3747057239436735 1105 66-67 0.5434736020802902 1105 68-69 0.7639609122247286 1105 70-71 0.6652488013952826 1105 72-73 0.4677121130515296 1105 74-75 0.23125010016667957 1105 76-77 0.7354797554420855 1105 78-79 0.8263081914763006 1105 80-81 1.0571685888759426 1105 82-83 1.3943888402179425 1105 84-85 1.1231404536864389 1105 86-87 0.6912299588456676 1105 88-89 0.8776222593657934 1105 90-91 1.1354881209727772 1105 92-93 1.0535488425273023 1105 94-95 1.0818211138980907 1105 96-97 0.9463390897172808 1105 98-99 1.0571083156704297 1105 100-101 0.898638852401362 1106 1 0.3677460619869173 1106 2 0.3539271783658222 1106 3 0.035242531995265836 1106 4 0.2502810105064981 1106 5 -0.11294270734489942 1106 6 0.07646392039572447 1106 7 0.13202758280800708 1106 8 0.18494325086358998 1106 9 -0.04580008285423531 1106 10-11 -0.12631727660001246 1106 12-13 -0.02119011937003279 1106 14-15 0.02260339213699325 1106 16-17 0.14411129805972678 1106 18-19 -0.02234646486760994 1106 20-21 -0.18723016094303802 1106 22-23 -0.24940932957110817 1106 24-25 -0.23472683877496792 1106 26-27 -0.47374518984992164 1106 28-29 -0.30438942625836063 1106 30-31 -0.3574090238025036 1106 32-33 -0.2213861757856037 1106 34-35 -0.4705297279609013 1106 36-37 -0.5866780607144833 1106 38-39 -0.19500878332851812 1106 40-41 -0.1122350845848672 1106 42-43 -0.09377019314860746 1106 44-45 0.44919055529481966 1106 46-47 0.5287976235041647 1106 48-49 0.011298454010006509 1106 50-51 0.37882152832496985 1106 52-53 -0.056523325003951186 1106 54-55 -0.17294628953013458 1106 56-57 -0.41596469854460594 1106 58-59 -0.1874310107119932 1106 60-61 -0.13685224925808015 1106 62-63 -0.5283512848705563 1106 64-65 -0.1395799903420425 1106 66-67 0.6077593163660069 1106 68-69 0.3996751979390112 1106 70-71 0.0366773728238563 1106 72-73 0.35521211305153244 1106 74-75 0.4973215287381052 1106 76-77 0.7747654697278001 1106 78-79 0.8959510486191569 1106 80-81 0.5964543031616572 1106 82-83 0.5247459830750856 1106 84-85 0.6731404536864396 1106 86-87 0.7590871017028107 1106 88-89 0.7811936879372219 1106 90-91 0.6444166924013501 1106 92-93 0.7678345568130176 1106 94-95 0.8086068281838052 1106 96-97 0.8320533754315669 1106 98-99 0.8053226013847166 1106 100-101 0.9272102809727869 1107 1 0.5746243688652264 1107 2 0.6163610407996813 1107 3 0.2230732198259524 1107 4 0.2596725448980308 1107 5 0.2296498852476887 1107 6 0.2730247669565742 1107 7 0.031498482278905726 1107 8 0.08071044663078197 1107 9 0.3788030917489422 1107 10-11 0.20675150646876972 1107 12-13 0.14871728803737483 1107 14-15 0.7005795826131802 1107 16-17 0.48458748853592226 1107 18-19 0.23367205365090626 1107 20-21 0.3692513205384458 1107 22-23 0.4777732101114367 1107 24-25 0.2478789813308495 1107 26-27 0.312828884224146 1107 28-29 0.22233015046121096 1107 30-31 0.2838608174673425 1107 32-33 0.6218677924683647 1107 34-35 0.5485840286528543 1107 36-37 0.6628589763225534 1107 38-39 0.8614726981529657 1107 40-41 0.9601855503357726 1107 42-43 0.28876948939107194 1107 44-45 -0.48235706375280074 1107 46-47 0.2610066182131554 1107 48-49 0.0030973958089504094 1107 50-51 0.4351045971080403 1107 52-53 0.4356724421918159 1107 54-55 0.377781223697383 1107 56-57 0.6287442961643919 1107 58-59 0.3275160792350924 1107 60-61 0.20838584598001475 1107 62-63 -0.08489890391817667 1107 64-65 -0.23137893214098426 1107 66-67 0.25894979255647854 1107 68-69 -0.12922691846310386 1107 70-71 -0.11424855310207249 1107 72-73 -0.17997307213365232 1107 74-75 0.12886914778572844 1107 76-77 0.10446123692356579 1107 78-79 0.5618240644921713 1107 80-81 0.4665601232674774 1107 82-83 0.5520607978899008 1107 84-85 0.7295557976017797 1107 86-87 0.9709918636075727 1107 88-89 1.0843021535456892 1107 90-91 0.7019563749410302 1107 92-93 0.47385307533153664 1107 94-95 0.4822840768610561 1107 96-97 0.667304698182889 1107 98-99 0.1734442945064103 1107 100-101 0.2053848841473922 1108 1 0.2677460619869194 1108 2 0.4217843212229653 1108 3 0.09595681770954911 1108 4 0.2324238676493593 1108 5 0.14062872122652692 1108 6 0.05860677753857857 1108 7 0.2784561542365793 1108 8 0.2956575365778775 1108 9 0.41491420286005365 1108 10-11 0.30225415197141103 1108 12-13 0.1288098806299729 1108 14-15 0.3208176778512808 1108 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0.3537844803107433 2316 52-53 0.007925400544358752 2316 54-55 0.7549212267709926 2316 56-57 0.24245261028764276 2316 58-59 0.42289797565433673 2316 60-61 0.5883996772386588 2316 62-63 0.5301771680043679 2316 64-65 1.0746760855322925 2316 66-67 0.5202222683517803 2316 68-69 0.08272943623184403 2316 70-71 0.286781107263689 2316 72-73 0.7406892914747694 2316 74-75 0.77997564847729 2316 76-77 -0.11415717401849435 2316 78-79 0.07069912212242002 2316 80-81 0.33202039681903983 2316 82-83 0.11400946855226479 2316 84-85 -0.13700329260876387 2316 86-87 0.04326019002527204 2316 88-89 -0.07348177145815527 2316 90-91 -0.006665109614061748 2316 92-93 -0.3284828705728735 2316 94-95 -0.2688531599608268 2316 96-97 -0.1016825463230262 2316 98-99 -0.8679880091665595 2316 100-101 -1.2735454985174286 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 7.0 5 22.0 6 41.0 7 125.0 8 248.0 9 474.0 10 905.0 11 1597.0 12 2206.0 13 3148.0 14 3941.0 15 5662.0 16 7802.0 17 10396.0 18 7880.0 19 3893.0 20 3039.0 21 3210.0 22 3543.0 23 3809.0 24 4103.0 25 4503.0 26 4933.0 27 5738.0 28 6598.0 29 7524.0 30 8747.0 31 10220.0 32 12434.0 33 15131.0 34 19767.0 35 26863.0 36 31390.0 37 32940.0 38 17528.0 39 1128.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.846438882109595 18.926158516622518 15.040254174231377 13.187148427036519 2 15.319526714407802 26.66136209589485 36.79714437273451 21.22196681696284 3 24.866290462789703 32.59602775854194 30.229405784502955 12.308275994165403 4 11.017870101930678 18.50629376811968 37.587996714052586 32.887839415897055 5 9.69406547426113 44.54356601938887 29.642057341544625 16.120311164805376 6 23.288004243156436 38.70001767981849 16.9240062468692 21.087971830155876 7 22.340660635884138 41.782936028523444 19.007646521495715 16.86875681409671 8 27.48622447476206 27.57720187406076 18.744659221498658 26.19191442967852 9 28.657620226308346 19.500309405940595 21.955666548797737 29.886403818953323 10-11 24.925873404666753 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16.73235234534706 36-37 18.451474360981322 25.081172269379593 41.06165777439726 15.405695595241822 38-39 18.595854082564752 25.902223237174766 40.60925391165984 14.892668768600641 40-41 16.62664004773727 27.6583322896945 39.850673699564624 15.864353963003616 42-43 15.432460308689727 29.72483147309095 36.78638523593767 18.056322982281653 44-45 17.392713624292337 27.850257649368494 35.49244363574752 19.264585090591655 46-47 18.604681143379125 29.480263800054146 33.679075189645424 18.235979866921305 48-49 18.878568705348016 29.053189157729364 32.7667976181925 19.301444518730122 50-51 17.478590765944123 30.701117884307262 33.79712333560471 18.023168014143906 52-53 17.721278868466612 32.17061401893256 30.72765111053814 19.38045600206269 54-55 15.733230191181347 30.28124654657973 31.879028990312 22.106494271926916 56-57 19.104281349508177 30.394690073169876 29.093544365109036 21.407484212212903 58-59 20.868708447669388 29.118584135987774 29.276229764820716 20.73647765152213 60-61 19.167561454916484 29.66135564563807 32.13262879885958 19.038454100585863 62-63 20.690233085808583 29.418058993399338 31.285913189533236 18.60579473125884 64-65 19.825239356509503 32.14376494251391 30.72843949502131 17.302556205955273 66-67 19.766437499654685 30.07213880412022 30.055747807013507 20.105675889211593 68-69 18.914090815334294 31.424035713233344 30.204496567168576 19.457376904263782 70-71 20.494852575553875 31.492292675739886 28.577874362327115 19.434980386379124 72-73 19.390893420160886 28.99950643840057 31.10248401449745 20.507116126941096 74-75 17.888477178300967 33.22258891475381 29.872631640981815 19.016302265963404 76-77 18.342292966181763 29.862373409504023 30.987253932385624 20.808079691928597 78-79 17.69690651775124 29.60209817514753 32.599622799973474 20.101372507127753 80-81 18.174601274227918 31.178798969271547 30.876358633087563 19.770241123412973 82-83 17.116818436330416 29.78737512156308 31.550408157251052 21.545398284855448 84-85 17.029776277329916 29.662454484675354 30.593296976327427 22.7144722616673 86-87 17.379508752283847 30.189117109683504 30.029063770849294 22.402310367183357 88-89 17.654058993007197 28.73643256626213 29.865302002048473 23.7442064386822 90-91 17.23622792765909 27.361229309195135 31.25679255223127 24.145750210914503 92-93 17.3832009782977 30.039190841719094 30.989310918922104 21.588297261061097 94-95 16.67341992234984 29.38531866771772 30.362944517707035 23.578316892225406 96-97 14.531962545216118 28.89392722691676 31.632126833508917 24.941983394358207 98-99 17.10909455917753 28.897082793843275 28.95362564946008 25.040196997519114 100-101 16.920566333475158 27.802059561896446 29.9767373754676 25.300636729160797 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1647.0 1 1224.5 2 1336.5 3 1682.0 4 1277.5 5 1137.5 6 1654.5 7 2862.0 8 3914.0 9 4129.0 10 3415.0 11 2496.0 12 2178.5 13 2074.5 14 1968.5 15 1844.5 16 1722.0 17 1651.5 18 1583.5 19 1497.5 20 1505.5 21 1553.0 22 1578.0 23 1669.0 24 1770.0 25 1857.5 26 2036.0 27 2236.0 28 2442.0 29 2813.5 30 3280.5 31 3509.0 32 3859.5 33 4500.0 34 4994.5 35 5335.0 36 5954.0 37 6490.0 38 6396.0 39 6204.0 40 5874.5 41 5441.0 42 5263.5 43 5154.0 44 5176.0 45 5274.5 46 6061.5 47 12026.0 48 29699.5 49 28312.0 50 11540.0 51 6462.5 52 3446.0 53 2473.0 54 2298.5 55 2194.5 56 2368.5 57 2603.5 58 2689.5 59 3005.5 60 3131.5 61 3037.5 62 2584.5 63 1463.0 64 673.0 65 553.0 66 446.0 67 312.0 68 255.0 69 202.5 70 168.0 71 146.0 72 124.5 73 127.0 74 107.5 75 84.5 76 63.5 77 43.0 78 43.0 79 35.0 80 19.5 81 16.0 82 13.5 83 7.0 84 4.0 85 2.5 86 4.5 87 4.0 88 0.5 89 2.5 90 2.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.99596310811209 2 0.011786545658131243 3 0.0044199546217992165 4 0.013628193417214251 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0029466364145328107 10-11 3.6832955181660134E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0022099773108996083 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.003314965966349412 34-35 3.6832955181660134E-4 36-37 0.0020258125349913074 38-39 0.0 40-41 0.0036832955181660136 42-43 0.009576568347231636 44-45 0.0011049886554498041 46-47 9.208238795415034E-4 48-49 0.00791908536405693 50-51 3.6832955181660134E-4 52-53 0.0022099773108996083 54-55 0.009576568347231636 56-57 0.001657482983174706 58-59 3.6832955181660134E-4 60-61 0.006077437604973922 62-63 0.0029466364145328107 64-65 0.014364852520847453 66-67 0.0020258125349913074 68-69 0.0 70-71 3.6832955181660134E-4 72-73 0.0 74-75 0.0 76-77 1.8416477590830067E-4 78-79 0.00810325013996523 80-81 0.013812358193122551 82-83 0.011786545658131243 84-85 0.00791908536405693 86-87 0.008839909243598433 88-89 0.027256386834428502 90-91 0.02173144355717948 92-93 0.0014733182072664054 94-95 0.00810325013996523 96-97 0.00810325013996523 98-99 0.007550755812240328 100-101 0.011234051330406341 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 271496.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.49407726080679 #Duplication Level Percentage of deduplicated Percentage of total 1 79.5651948321486 28.240931726434276 2 10.607585741711203 7.530129357338598 3 3.7264567010844187 3.9680142617202465 4 1.7112021999688685 2.4295017237823022 5 0.9515903076843252 1.6887909950791171 6 0.595651948321486 1.268526976456375 7 0.43791833134436775 1.0880454960662402 8 0.3154672339542365 0.8957774700179744 9 0.24905307943755514 0.795591831923859 >10 1.510922015254501 10.010460559271591 >50 0.15773361697711824 3.9952706485546745 >100 0.12037565506148498 8.267525120075433 >500 0.023867586779432366 5.846126646433096 >1k 0.023867586779432366 16.547205115360818 >5k 0.003113163492969439 7.4281020714854 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7306 2.6910157055720894 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6582 2.42434511005687 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6279 2.31274125585644 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4405 1.622491675752129 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3656 1.3466128414414946 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3407 1.2548987830391607 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3363 1.2386922827592304 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3116 1.1477148834605297 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2589 0.9536052096531809 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2024 0.7454990128768011 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1883 0.6935645460706604 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1766 0.650469988508118 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1720 0.6335268291245543 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1654 0.6092170787046586 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1640 0.6040604649792263 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1496 0.5510210095176357 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1421 0.5233962931313906 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1361 0.5012965200223944 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1340 0.4935615994342458 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1315 0.4843533606388307 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1223 0.45046704187170344 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1201 0.4423637917317382 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1157 0.42615729145180775 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1119 0.41216076848277694 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1063 0.39153431358104723 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1006 0.37053952912750093 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 989 0.36427792674661874 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 957 0.3524913810884875 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 930 0.34254648318943925 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 888 0.327076642013142 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 883 0.325234994254059 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 875 0.3222883578395262 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 716 0.2637239591006866 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 683 0.25156908389073873 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 678 0.2497274361316557 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 673 0.24788578837257272 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 662 0.24383416330259008 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 653 0.2405191973362407 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 642 0.23646757226625806 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 620 0.22836432212629282 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 617 0.22725933347084304 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 604 0.22247104929722725 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 595 0.21915608333087777 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 586 0.21584111736452838 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 551 0.20294958305094735 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 543 0.20000294663641452 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 518 0.1907947078409995 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 506 0.18637475321920027 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 503 0.18526976456375047 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 499 0.18379644635648407 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 495 0.18232312814921767 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 449 0.165379968765654 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 445 0.1639066505583876 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 391 0.14401685476029114 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 365 0.1344402864130595 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 361 0.1329669682057931 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 317 0.11676046792586264 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 307 0.11307717240769662 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 303 0.1116038542004302 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 298 0.10976220644134721 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 294 0.1082888882340808 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 292 0.1075522291304476 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 291 0.10718389957863099 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 290 0.1068155700268144 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 290 0.1068155700268144 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 284 0.10460559271591478 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 283 0.10423726316409819 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 277 0.10202728585319858 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 277 0.10202728585319858 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 274 0.10092229719774878 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 272 0.10018563809411557 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.8416477590830067E-4 0.0 12-13 0.0 0.0 0.0 3.6832955181660134E-4 0.0 14-15 0.0 0.0 0.0 3.6832955181660134E-4 0.0 16-17 0.0 0.0 0.0 3.6832955181660134E-4 0.0 18-19 0.0 0.0 0.0 3.6832955181660134E-4 0.0 20-21 0.0 0.0 0.0 5.524943277249021E-4 0.0 22-23 0.0 0.0 0.0 0.0020258125349913074 0.0 24-25 0.0 0.0 0.0 0.0029466364145328107 0.0 26-27 0.0 0.0 0.0 0.005156613725432419 0.0 28-29 0.0 0.0 0.0 0.006261602380882223 0.0 30-31 0.0 0.0 0.0 0.009392403571323335 0.0 32-33 0.0 0.0 0.0 0.01602233550402216 0.0 34-35 0.0 0.0 0.0 0.020442290125821376 0.0 36-37 0.0 0.0 0.0 0.024678079971712293 0.0 38-39 0.0 0.0 0.0 0.030387188024869612 0.0 40-41 0.0 0.0 0.0 0.04198956890709256 0.0 42-43 0.0 0.0 0.0 0.054512773668857 0.0 44-45 0.0 0.0 0.0 0.0823216548310104 0.0 46-47 0.0 0.0 0.0 0.14898930370981522 0.0 48-49 0.0 0.0 0.0 0.20221292394731416 0.0 50-51 0.0 0.0 0.0 0.25359489642573 0.0 52-53 0.0 0.0 0.0 0.3075551757668621 0.0 54-55 0.0 0.0 0.0 0.3679612222647848 0.0 56-57 0.0 0.0 0.0 0.43260305860859827 0.0 58-59 0.0 0.0 0.0 0.5513893390694522 0.0 60-61 0.0 0.0 0.0 0.7059035860565165 0.0 62-63 0.0 0.0 0.0 0.8646536228894717 0.0 64-65 0.0 0.0 0.0 0.9718375224681026 0.0 66-67 0.0 0.0 0.0 1.1537923210655037 0.0 68-69 0.0 0.0 0.0 1.3703700975336655 0.0 70-71 0.0 0.0 0.0 1.506836196481716 0.0 72-73 0.0 0.0 0.0 1.7556428087338303 0.0 74-75 0.0 0.0 0.0 1.9438592097121137 0.0 76-77 0.0 0.0 0.0 2.08621858148923 0.0 78-79 0.0 0.0 0.0 2.296534755576509 0.0 80-81 0.0 0.0 0.0 2.4742537643280196 0.0 82-83 0.0 0.0 0.0 2.7184562571824262 0.0 84-85 0.0 0.0 0.0 3.092679081828093 0.0 86-87 0.0 0.0 0.0 3.6495933641747946 0.0 88-89 0.0 0.0 0.0 4.242788107375431 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATATG 45 1.5147163E-5 52.76693 3 ATGGGCG 60 1.4920424E-6 47.481487 5 GCTTATG 55 4.66985E-5 43.614876 1 AGGCTTA 45 9.630521E-4 42.20577 6 CGTCACT 70 4.0130017E-6 41.122597 1 TGGCTAA 25 0.0016166964 37.99219 50-51 GAGTACG 55 0.0024530848 34.891903 1 AGTATAT 70 2.0472538E-4 33.9341 2 CTTACTA 70 2.0539148E-4 33.915348 9 GTCACTA 100 1.2927012E-6 33.255417 2 CTTATGT 60 0.003942534 31.671827 2 CATGGAT 60 0.003949655 31.660158 4 TATATGG 60 0.003949655 31.660158 4 GGGATAG 90 2.4126468E-5 31.654325 7 GATATAG 65 0.005558427 29.523916 1 TATGTAC 65 0.005842178 29.224762 4 ACATAGA 100 4.6143454E-5 28.785818 1 GAGGGTT 210 4.1836756E-11 27.13228 9 CACTGTA 70 0.00839643 27.132278 7 ATATAGG 90 8.921374E-4 26.393188 2 >>END_MODULE