##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936697_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 275292 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.65819929384072 31.0 31.0 34.0 30.0 34.0 2 31.52334975226305 31.0 31.0 34.0 30.0 34.0 3 31.514540923819073 31.0 31.0 34.0 28.0 34.0 4 35.21460122342821 37.0 35.0 37.0 33.0 37.0 5 35.233446667538466 37.0 35.0 37.0 33.0 37.0 6 35.48884457230868 37.0 35.0 37.0 33.0 37.0 7 35.421810295976634 37.0 35.0 37.0 33.0 37.0 8 35.37412274966218 37.0 35.0 37.0 33.0 37.0 9 36.77774871772518 39.0 37.0 39.0 32.0 39.0 10-11 36.68671083794662 39.0 37.0 39.0 32.0 39.0 12-13 36.58586700666928 39.0 37.0 39.0 32.0 39.0 14-15 37.3070648620374 39.0 36.5 41.0 32.0 41.0 16-17 37.33923978902402 39.0 36.5 41.0 32.0 41.0 18-19 37.62792780029932 39.5 37.0 41.0 32.0 41.0 20-21 37.70032002382924 40.0 37.0 41.0 32.5 41.0 22-23 37.563403586010494 40.0 36.5 41.0 32.0 41.0 24-25 37.534350798424946 40.0 36.5 41.0 32.0 41.0 26-27 37.40281228659023 39.5 36.0 41.0 32.0 41.0 28-29 37.22928563125699 39.0 36.0 41.0 32.0 41.0 30-31 36.82359639945948 38.5 35.0 40.0 32.0 41.0 32-33 36.32977710939657 38.0 35.0 40.0 31.0 41.0 34-35 35.90822108888017 38.0 35.0 40.0 30.0 41.0 36-37 35.51854031355796 38.0 35.0 40.0 30.0 41.0 38-39 35.10659408918531 37.0 35.0 40.0 29.5 41.0 40-41 34.769877075977504 37.0 35.0 40.0 27.5 41.0 42-43 34.247193888670935 37.0 34.5 40.0 24.5 41.0 44-45 33.49575178356073 36.5 33.0 40.0 20.5 41.0 46-47 32.382704546445225 36.0 33.0 40.0 5.5 41.0 48-49 31.608546561469275 36.0 31.5 40.0 2.0 41.0 50-51 30.48060967990352 35.5 29.5 39.0 2.0 40.0 52-53 30.557538904145417 35.0 30.5 39.0 2.0 40.0 54-55 30.89765594350726 35.0 31.0 39.5 2.0 41.0 56-57 30.687375586649814 35.0 31.0 39.5 2.0 41.0 58-59 30.40750366883164 35.0 30.5 39.0 2.0 41.0 60-61 29.89686587332723 35.0 28.5 39.0 2.0 41.0 62-63 29.4908478996847 35.0 28.0 39.0 2.0 40.5 64-65 29.01215981575927 35.0 28.0 38.0 2.0 40.0 66-67 28.460576406143296 35.0 26.0 37.5 2.0 40.0 68-69 27.97900411199744 35.0 26.0 37.0 2.0 39.0 70-71 27.394582842944946 34.0 24.5 36.0 2.0 39.0 72-73 26.912387573921507 34.0 23.0 35.5 2.0 38.5 74-75 26.46355869404124 34.0 20.5 35.0 2.0 37.0 76-77 25.96271595251587 33.5 19.0 35.0 2.0 37.0 78-79 25.464492248230968 33.0 11.0 35.0 2.0 36.0 80-81 24.878254725891054 33.0 4.5 35.0 2.0 36.0 82-83 24.357950830391005 32.5 2.0 35.0 2.0 35.5 84-85 23.943171250889964 32.0 2.0 35.0 2.0 35.0 86-87 23.56902488993505 32.0 2.0 35.0 2.0 35.0 88-89 22.96964314255409 31.5 2.0 35.0 2.0 35.0 90-91 22.346831727765426 31.0 2.0 35.0 2.0 35.0 92-93 21.973255670342763 31.0 2.0 35.0 2.0 35.0 94-95 21.49954230417157 31.0 2.0 35.0 2.0 35.0 96-97 20.854516295424496 30.0 2.0 34.0 2.0 35.0 98-99 20.1759150284062 29.0 2.0 34.0 2.0 35.0 100-101 18.27696046379844 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5968408853719431 1101 2 0.7064673977691172 1101 3 0.7502298184026479 1101 4 0.8525427850633704 1101 5 0.7142757084123375 1101 6 0.3407176531349023 1101 7 0.4708473734390779 1101 8 0.629597731296208 1101 9 0.6568239204098418 1101 10-11 0.7562089614200218 1101 12-13 0.9662745325545856 1101 14-15 1.1396884208822584 1101 16-17 1.1597275794727295 1101 18-19 0.9255885282331491 1101 20-21 0.4677667476453493 1101 22-23 0.670098641074695 1101 24-25 0.6136108758950485 1101 26-27 0.80292205566424 1101 28-29 0.5846315332264851 1101 30-31 0.6972954795895632 1101 32-33 0.7806350386163388 1101 34-35 0.6913711636650461 1101 36-37 0.7837231215849059 1101 38-39 0.8095873381342784 1101 40-41 0.6838074064964061 1101 42-43 0.48775301316789665 1101 44-45 0.8542578679655932 1101 46-47 1.1772903073515977 1101 48-49 1.890815455756023 1101 50-51 1.758015230862135 1101 52-53 1.2236660510257629 1101 54-55 1.51347761051721 1101 56-57 1.5628894309160835 1101 58-59 1.0611437159403643 1101 60-61 0.8521625007458589 1101 62-63 0.764644625726774 1101 64-65 0.5797951859040964 1101 66-67 0.8844569143008449 1101 68-69 0.7008576679764822 1101 70-71 0.6688740419915966 1101 72-73 0.6740946665892054 1101 74-75 0.894335579141579 1101 76-77 0.930251737714272 1101 78-79 0.8599495534801065 1101 80-81 0.8364777116530462 1101 82-83 0.7023530603070931 1101 84-85 0.5750853325719163 1101 86-87 0.3862352484393661 1101 88-89 0.545953759322007 1101 90-91 -0.22639350042829065 1101 92-93 -0.4715187292244849 1101 94-95 -0.41570759226934584 1101 96-97 -0.4461221186457607 1101 98-99 -0.774599886372318 1101 100-101 -0.520218609936471 1102 1 0.3461869778759308 1102 2 0.4326555955362643 1102 3 0.2924307753404456 1102 4 0.29311694774279573 1102 5 0.3383905409482182 1102 6 0.23169053989726507 1102 7 0.2404805472827789 1102 8 0.27636009174278087 1102 9 0.5093279076506718 1102 10-11 0.32080226285543034 1102 12-13 0.26770993925314457 1102 14-15 0.6029739073256408 1102 16-17 0.5608121089783111 1102 18-19 0.400708145458033 1102 20-21 0.31969657220674463 1102 22-23 0.25673340981472137 1102 24-25 0.15826797318372599 1102 26-27 0.34202891371846533 1102 28-29 0.48488671663956495 1102 30-31 0.3328297698607017 1102 32-33 0.5262809716306975 1102 34-35 0.5109564906187885 1102 36-37 0.6053818137699167 1102 38-39 0.7315012137323649 1102 40-41 0.4570131481232025 1102 42-43 0.68025700040873 1102 44-45 0.44858003702459825 1102 46-47 1.0583748368571833 1102 48-49 1.3003848337464596 1102 50-51 1.2147935402720265 1102 52-53 0.8275257001485663 1102 54-55 0.9933819167373024 1102 56-57 0.827737915764569 1102 58-59 1.2194850237553538 1102 60-61 1.0587015757059852 1102 62-63 1.314086412010667 1102 64-65 1.7112784394926095 1102 66-67 1.5922719063263635 1102 68-69 1.404653521246022 1102 70-71 1.6050462907954248 1102 72-73 1.26844873357485 1102 74-75 0.48821117722770424 1102 76-77 0.5559933645085309 1102 78-79 0.4624694896842527 1102 80-81 0.3877057818284868 1102 82-83 0.8661489135766267 1102 84-85 0.6888971348047725 1102 86-87 0.3433325371156002 1102 88-89 -0.11143061069394022 1102 90-91 -0.1416088114330769 1102 92-93 -0.2833847579326161 1102 94-95 -0.26948749657556803 1102 96-97 0.13457963574020582 1102 98-99 0.3773299381890851 1102 100-101 0.6472455048960661 1103 1 0.13717664717112044 1103 2 0.2763501978958196 1103 3 0.2520860109905456 1103 4 0.44653708344790743 1103 5 0.15694913254917253 1103 6 0.4707271558273334 1103 7 0.19772732275804827 1103 8 0.2755654221419448 1103 9 0.12426769614629762 1103 10-11 0.5039124774162289 1103 12-13 0.45369930290618044 1103 14-15 0.4907115441005132 1103 16-17 1.009452001392276 1103 18-19 0.6551735773303946 1103 20-21 0.812923542070429 1103 22-23 0.32769129232588057 1103 24-25 0.7947005179603082 1103 26-27 0.44847796317136357 1103 28-29 0.06890774481977502 1103 30-31 0.6786638672994201 1103 32-33 0.6078507497344887 1103 34-35 0.5432812048433746 1103 36-37 0.8802641415405574 1103 38-39 1.1165369738643989 1103 40-41 0.9752233076684078 1103 42-43 0.7823048028416366 1103 44-45 1.113345609492356 1103 46-47 1.2735652519192229 1103 48-49 1.2579678155913108 1103 50-51 1.5378695229115529 1103 52-53 1.5620632635693177 1103 54-55 1.82541932733697 1103 56-57 1.6154266497947667 1103 58-59 2.055404089712926 1103 60-61 2.3470627224753464 1103 62-63 1.8960351863856317 1103 64-65 1.7430957877412823 1103 66-67 1.4456859291883966 1103 68-69 1.405729381628685 1103 70-71 1.4367169308100962 1103 72-73 1.5116176314292424 1103 74-75 1.2278040618783557 1103 76-77 1.5198114462983732 1103 78-79 1.6253217422669266 1103 80-81 1.3201710914439886 1103 82-83 1.0167021258756712 1103 84-85 1.258094518507928 1103 86-87 1.6180375924368313 1103 88-89 1.8915096668291334 1103 90-91 1.8171478362837767 1103 92-93 1.9631217522452644 1103 94-95 2.074213218627076 1103 96-97 2.452484153131241 1103 98-99 2.2904175352304676 1103 100-101 2.2908361889232083 1104 1 0.33488950342056256 1104 2 0.36729989860161893 1104 3 0.6165102046830349 1104 4 0.47191008443066806 1104 5 0.3546619887986182 1104 6 0.2440676364848855 1104 7 -0.028306805715104133 1104 8 0.3399806816791582 1104 9 0.7147260183119357 1104 10-11 0.4093891146001809 1104 12-13 0.4498576161376633 1104 14-15 0.9099914511852987 1104 16-17 0.46902494877009815 1104 18-19 0.49525219789682495 1104 20-21 0.5730414729200746 1104 22-23 0.1260027369787835 1104 24-25 0.12553228781646197 1104 26-27 0.1376140303562181 1104 28-29 0.2058236744186317 1104 30-31 0.20086524315933474 1104 32-33 0.385390283371585 1104 34-35 0.6294064617003343 1104 36-37 0.5821313799931644 1104 38-39 0.4932903018372343 1104 40-41 0.4520391747281778 1104 42-43 -0.10994928453440167 1104 44-45 0.22980232351005014 1104 46-47 0.5783092883705834 1104 48-49 0.7675720325126072 1104 50-51 0.4901164629633712 1104 52-53 0.754728322088031 1104 54-55 0.5070507040903003 1104 56-57 0.40685879488544785 1104 58-59 0.9156292304258749 1104 60-61 0.7984162469996043 1104 62-63 1.3631128241949781 1104 64-65 1.2560056421145518 1104 66-67 1.388686018529949 1104 68-69 0.9985999257187395 1104 70-71 1.5349479680655236 1104 72-73 1.0734419859365225 1104 74-75 1.1600831648891656 1104 76-77 1.3648038522663875 1104 78-79 1.2942219477524972 1104 80-81 1.4382359534112865 1104 82-83 1.4293260872801206 1104 84-85 1.4960110734976588 1104 86-87 1.663691125895241 1104 88-89 1.5824772358454844 1104 90-91 1.5489444949097013 1104 92-93 1.5371858994801428 1104 94-95 1.1101731936114412 1104 96-97 1.0340976615740196 1104 98-99 1.1874513956789663 1104 100-101 1.1774170876992258 1105 1 0.28100235567059784 1105 2 0.3945277850352795 1105 3 0.35133254239345746 1105 4 0.28905082575913355 1105 5 0.30696359813993723 1105 6 0.4215315684614538 1105 7 0.31658416372460607 1105 8 0.38434666467330203 1105 9 0.6033680621886361 1105 10-11 0.437370759910344 1105 12-13 0.43010781118930197 1105 14-15 -3.575259506831685E-4 1105 16-17 0.4198785476381346 1105 18-19 0.2648730704057769 1105 20-21 0.1690663866345119 1105 22-23 0.3146408137035053 1105 24-25 0.4646774988028284 1105 26-27 0.5470703982963343 1105 28-29 0.642084765914376 1105 30-31 0.3381815970821833 1105 32-33 0.1810026132799436 1105 34-35 0.5863760865399996 1105 36-37 0.6845698560778501 1105 38-39 0.6772735869035529 1105 40-41 0.7587992017048109 1105 42-43 1.3638278408672733 1105 44-45 1.4624757872304386 1105 46-47 1.213470156383437 1105 48-49 0.8299687212630822 1105 50-51 0.778363114025904 1105 52-53 0.5829440293651125 1105 54-55 0.8447214569235086 1105 56-57 1.0668474223830984 1105 58-59 0.8218854291008455 1105 60-61 0.18852613710949484 1105 62-63 0.7903420330126316 1105 64-65 0.7382592489168935 1105 66-67 0.8171119848620485 1105 68-69 0.9056098832702126 1105 70-71 0.5012271762219882 1105 72-73 0.33466572008444473 1105 74-75 0.5620283917441391 1105 76-77 0.3214693287874617 1105 78-79 0.8146197208578521 1105 80-81 0.8805932351187522 1105 82-83 1.0905709795719396 1105 84-85 1.2198047286992555 1105 86-87 1.4900422717409718 1105 88-89 1.3601382030371347 1105 90-91 1.5708365619281217 1105 92-93 1.7581694886947794 1105 94-95 1.5894712721874988 1105 96-97 0.7809536936286072 1105 98-99 0.594930799548262 1105 100-101 0.6965519840904406 1106 1 0.3033824576786408 1106 2 0.299984059043787 1106 3 0.26808164629006015 1106 4 0.3960950335266489 1106 5 0.24282957447510256 1106 6 0.4160072034422484 1106 7 0.35628250769983083 1106 8 0.39542108943273035 1106 9 0.5200914713642248 1106 10-11 0.4396447427205743 1106 12-13 0.4838740149227405 1106 14-15 0.6294457809599052 1106 16-17 0.7760459230508587 1106 18-19 0.5748606084660892 1106 20-21 0.6609340074318268 1106 22-23 0.4089404398453169 1106 24-25 0.3208577215760613 1106 26-27 0.22956715433780772 1106 28-29 0.6993775701077567 1106 30-31 0.5694533572990466 1106 32-33 0.6746046277460707 1106 34-35 0.8203714871849357 1106 36-37 0.6737716495693746 1106 38-39 0.7474779884092726 1106 40-41 0.6066479111874443 1106 42-43 0.48603835771658055 1106 44-45 0.809521536681217 1106 46-47 0.45688267228204893 1106 48-49 0.1183469989459347 1106 50-51 0.12246039423968469 1106 52-53 0.031708755652093856 1106 54-55 0.38630193921342126 1106 56-57 0.11551964767441447 1106 58-59 0.4923763267459229 1106 60-61 0.31130193782123783 1106 62-63 0.4794294849956202 1106 64-65 0.35624940783874237 1106 66-67 -0.019328268487900147 1106 68-69 0.26722453953908243 1106 70-71 0.47736320407506483 1106 72-73 0.5902318390253107 1106 74-75 0.46498585413349147 1106 76-77 0.7046354323114876 1106 78-79 0.918066667682627 1106 80-81 1.3459439038238656 1106 82-83 1.2667982236846385 1106 84-85 1.1293978527919073 1106 86-87 1.2893086874558612 1106 88-89 1.2686842672482435 1106 90-91 1.2131018085332066 1106 92-93 0.8305129757253695 1106 94-95 0.9430242097364818 1106 96-97 0.6044116891834186 1106 98-99 0.9730157719906707 1106 100-101 1.2542880222214094 1107 1 0.23232540085646036 1107 2 0.27938781068953134 1107 3 0.2721878603606882 1107 4 0.22282583534641986 1107 5 0.2008158349524649 1107 6 -0.0636246712074211 1107 7 0.14019136278306377 1107 8 -0.011667669969192218 1107 9 -0.24498094139502058 1107 10-11 0.0833817885928525 1107 12-13 -0.11058194430189872 1107 14-15 0.14259218378602867 1107 16-17 0.08074656049171125 1107 18-19 -0.004747802103175047 1107 20-21 -0.05150065162205664 1107 22-23 0.041753652729703106 1107 24-25 -0.07226990998573513 1107 26-27 -0.3550599623177817 1107 28-29 -0.2227477541527989 1107 30-31 0.15690919920328383 1107 32-33 0.20224010022140249 1107 34-35 0.2795896118834875 1107 36-37 0.31656361442539804 1107 38-39 0.5189313274782705 1107 40-41 0.18830291099191498 1107 42-43 0.43950126491614583 1107 44-45 0.5814506751584005 1107 46-47 1.0783092883705834 1107 48-49 1.2217844867250598 1107 50-51 0.682424155271061 1107 52-53 0.8792704466301551 1107 54-55 0.8477100447496433 1107 56-57 1.0680309560576084 1107 58-59 0.8057391205357654 1107 60-61 0.9284528770362357 1107 62-63 0.49314945423160594 1107 64-65 0.8237712098801211 1107 66-67 0.9399680698120001 1107 68-69 0.3429222700410861 1107 70-71 0.36461829773585563 1107 72-73 0.4342478467423838 1107 74-75 0.6124641172701182 1107 76-77 0.5314705189330517 1107 78-79 0.4645516180821687 1107 80-81 0.7001407153160493 1107 82-83 1.365223523177555 1107 84-85 1.4483920258786078 1107 86-87 1.0483065105106242 1107 88-89 1.2125138658821122 1107 90-91 1.6588346047998108 1107 92-93 1.1763800386742815 1107 94-95 1.3464369298751784 1107 96-97 1.3106544381307934 1107 98-99 1.7350704432980102 1107 100-101 1.5308969411790798 1108 1 -0.008419094500581537 1108 2 0.1928679345017592 1108 3 0.2594146557055623 1108 4 0.0450687830506169 1108 5 0.15579197812049017 1108 6 0.03528994431236043 1108 7 -0.4130573565173137 1108 8 0.053468915462595135 1108 9 0.32329825788048794 1108 10-11 0.06483022945088379 1108 12-13 0.22286628029024058 1108 14-15 0.13262367750756 1108 16-17 0.40281379215304014 1108 18-19 -0.17390828491679855 1108 20-21 -0.021236942391560376 1108 22-23 0.2694478527486268 1108 24-25 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-0.5016336689348861 2316 58-59 -0.959325732643844 2316 60-61 -0.639342715349521 2316 62-63 -0.6906087274773967 2316 64-65 -1.0712927425608818 2316 66-67 -1.3096190916604087 2316 68-69 -2.2012064154810425 2316 70-71 -2.4170201457878377 2316 72-73 -1.9741095062870997 2316 74-75 -1.2613574163874794 2316 76-77 -0.806193865862479 2316 78-79 -1.9530683302754852 2316 80-81 -1.4244239276912154 2316 82-83 -0.9802847936422374 2316 84-85 -0.6435733857589341 2316 86-87 -0.9024756308452844 2316 88-89 -1.3431893107227033 2316 90-91 -0.8797019952121978 2316 92-93 -1.8687169408489268 2316 94-95 -2.218926668275305 2316 96-97 -3.2153844137277297 2316 98-99 -4.309763820798548 2316 100-101 -4.765933960160016 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 453.0 3 167.0 4 129.0 5 147.0 6 175.0 7 236.0 8 284.0 9 437.0 10 644.0 11 1126.0 12 2006.0 13 2824.0 14 3512.0 15 4869.0 16 6698.0 17 9393.0 18 6990.0 19 3516.0 20 3215.0 21 3502.0 22 3862.0 23 4326.0 24 4587.0 25 4755.0 26 5187.0 27 5738.0 28 6452.0 29 7406.0 30 8593.0 31 10065.0 32 12058.0 33 15241.0 34 20323.0 35 28162.0 36 32001.0 37 31964.0 38 21125.0 39 3124.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.204126723404876 17.878712997216386 13.942409023846036 13.974751255532702 2 15.277429575289855 24.744661601683 38.13562093284355 21.842287890183595 3 24.069586196121747 33.5366806308974 29.46622242730546 12.927510745675388 4 10.844227351526978 17.718271058437004 38.71256146624944 32.72494012378658 5 10.093270426077918 43.309682531605084 29.91632559593478 16.680721446382226 6 23.243465240019773 37.83800482656354 18.089073357950745 20.829456575465937 7 23.17284592263603 41.89670023733286 18.563936309019738 16.366517531011375 8 26.60946545577445 27.15058745716269 19.091903521108847 27.148043565954016 9 29.856300743572785 19.16208142231009 21.59631920569273 29.385298628424394 10-11 24.808694909494655 28.00259495292559 22.30151245050418 24.88719768707557 12-13 33.03756709708134 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38.40517319673769 16.257969592937716 38-39 19.200210709874302 27.562123083630024 37.535784349342435 15.701881857153237 40-41 16.69248834391931 29.34807681479773 37.692141431350365 16.2672934099326 42-43 15.777830248651398 31.159162322683763 34.42722996167608 18.635777466988756 44-45 17.134180452329577 29.66896246085889 33.38364464593187 19.813212440879667 46-47 18.42087100559045 30.634128976246956 31.971622755556844 18.97337726260575 48-49 18.63347494183396 30.50080006683873 31.159924370526298 19.70580062080101 50-51 17.44042224333116 31.93832719135898 32.37495527468157 18.246295290628296 52-53 17.399367196666702 33.5215033256685 29.331633264676714 19.74749621298809 54-55 15.589586356841467 31.424383385977944 30.894546696285698 22.091483560894893 56-57 18.991133498880107 31.773173066617744 27.803683567090946 21.432009867411203 58-59 20.06165046691964 30.486646334485574 28.382102110175232 21.069601088419557 60-61 19.03354597767961 30.331608928156246 31.32665368518949 19.308191408974658 62-63 20.57528031110764 30.706077024933293 29.91395861222162 18.804684051737446 64-65 19.469145489153338 33.004046612324366 30.11166325211049 17.41514464641181 66-67 19.254228544857448 30.89889273155279 29.26232962306373 20.584549100526026 68-69 18.42803216090626 32.1532765830672 29.792618105587465 19.62607315043907 70-71 20.128380477013028 31.536587891040067 28.03954583680713 20.29548579513978 72-73 18.904260864669098 29.67366488124104 30.395281971923595 21.02679228216627 74-75 17.52113310495897 33.43026529448305 29.521322004221172 19.527279596336808 76-77 18.02301715024721 30.178404710959665 30.489005460034218 21.309572678758904 78-79 17.262605347282765 30.098445219412962 32.32000145306597 20.318947980238303 80-81 17.89781138573486 31.634185904154354 30.416815765143106 20.05118694496768 82-83 16.76496284604221 30.243557770726206 31.01787858257333 21.973600800658254 84-85 17.147543902035835 29.96237876643772 30.232139905211202 22.657937426315243 86-87 17.47158755501307 30.51452090723657 30.331613238373922 21.682278299376442 88-89 17.850513123240397 28.757061120697486 29.71846335482699 23.67396240123513 90-91 17.184409413636587 27.83786778261525 31.302503074125944 23.675219729622224 92-93 17.303231174191296 30.480979361685506 31.02795555159942 21.187833912523775 94-95 16.608237272988184 29.887452272570396 30.596597411184295 22.90771304325713 96-97 14.587972869385782 29.319445907702146 31.826339365184314 24.266241857727756 98-99 16.763009980707118 29.753915801635717 29.229447264297992 24.253626953359177 100-101 16.960278786160984 28.39080209162297 30.800565421358826 23.848353700857217 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 790.0 1 630.0 2 884.5 3 1229.5 4 1003.0 5 955.5 6 1432.5 7 2622.0 8 3959.0 9 4350.5 10 3433.5 11 2463.5 12 2294.0 13 2270.0 14 2187.5 15 1992.5 16 1752.5 17 1652.0 18 1607.0 19 1543.5 20 1527.0 21 1536.5 22 1610.0 23 1688.5 24 1775.0 25 1940.5 26 2159.0 27 2450.5 28 2711.5 29 2950.5 30 3299.0 31 3739.0 32 4139.5 33 4618.5 34 5125.5 35 5453.0 36 6065.5 37 6687.5 38 6601.5 39 6265.0 40 5827.0 41 5378.5 42 5203.0 43 5043.5 44 5093.0 45 5282.5 46 6292.5 47 12071.5 48 28991.0 49 28443.5 50 12265.0 51 6694.5 52 3832.5 53 2877.5 54 2554.5 55 2406.5 56 2563.5 57 2642.5 58 2660.0 59 2896.5 60 3219.5 61 3574.5 62 3147.0 63 1690.5 64 771.5 65 629.0 66 503.5 67 353.5 68 239.0 69 177.5 70 144.0 71 128.0 72 130.0 73 146.5 74 123.5 75 83.5 76 56.5 77 38.5 78 30.5 79 23.0 80 18.5 81 12.0 82 8.0 83 8.0 84 5.5 85 1.5 86 2.0 87 1.5 88 1.0 89 1.0 90 0.5 91 0.5 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0399575723232059 2 0.025064295366374615 3 0.023611292736439853 4 0.051218342705200294 5 0.06465861703209683 6 0.05376109730758613 7 0.054850849280037195 8 0.044679830870493875 9 0.048675588102814464 10-11 0.05176321869142583 12-13 0.04758583613036339 14-15 0.04141057495314067 16-17 0.04704096014413786 18-19 0.05303459599261875 20-21 0.012895398340670997 22-23 0.019252284846635572 24-25 0.005812010519739041 26-27 0.013077023669412841 28-29 0.005630385190997195 30-31 0.009989393080801476 32-33 0.003995757232320591 34-35 0.006538511834706421 36-37 0.009807767752059632 38-39 0.011624021039478082 40-41 0.0023611292736439855 42-43 0.00254275460238583 44-45 0.00217950394490214 46-47 3.6325065748369007E-4 48-49 9.081266437092251E-4 50-51 1.8162532874184504E-4 52-53 0.0032692559173532103 54-55 0.006720137163448266 56-57 0.015438152943056827 58-59 0.011079145053252547 60-61 0.01017101840954332 62-63 0.006356886505964576 64-65 0.0 66-67 0.007265013149673801 68-69 0.018525783531668193 70-71 0.005993635848480886 72-73 0.0027243799311276753 74-75 0.004722258547287971 76-77 0.006901762492190111 78-79 0.00435900788980428 80-81 0.009626142423317786 82-83 0.006720137163448266 84-85 0.01761765688795897 86-87 0.005993635848480886 88-89 0.006175261177222731 90-91 0.0038141319035787454 92-93 0.018707408860410037 94-95 0.01126077038199439 96-97 0.01126077038199439 98-99 0.022158290106505094 100-101 0.035961815090885314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 275292.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.84461752974023 #Duplication Level Percentage of deduplicated Percentage of total 1 79.49883319294342 29.29104103054618 2 10.729977486009671 7.906838331495 3 3.6631982574958504 4.0490741619913635 4 1.7640000971400676 2.599756356062016 5 0.9589980668847897 1.7666958493065161 6 0.6239986926761165 1.379459590242566 7 0.47099901179139686 1.2147644912437738 8 0.3449992761128123 1.0169093101131053 9 0.23099951530928925 0.7659979912013523 >10 1.413997033105097 9.767119167269387 >50 0.14799968946220238 4.018634001939234 >100 0.10399978178425032 7.853413748174088 >500 0.022999951740747662 5.780539915322696 >1k 0.021999953838976027 15.826940697469674 >5k 0.0029999937053149127 6.762815357623053 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7161 2.6012379582407044 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5646 2.050913212152914 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5548 2.0153146477195123 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3655 1.3276811531028871 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3653 1.3269546517879198 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3642 1.3229588945555992 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3164 1.1493250802783954 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2977 1.0813972073289453 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2729 0.9913110442729901 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2456 0.8921436147799426 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2137 0.7762666550426457 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1643 0.5968208302457028 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1570 0.5703035322493933 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1419 0.5154526829693562 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1414 0.5136364296819377 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1409 0.5118201763945193 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1404 0.5100039231071009 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1399 0.5081876698196824 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1361 0.49438414483530213 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1328 0.4823968731383404 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1205 0.43771704226784647 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1153 0.41882800807869464 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1127 0.4093834909841187 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1100 0.3995757232320591 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1011 0.36724641471601066 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 998 0.3625241561687227 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 865 0.31421181872339193 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 849 0.3083998082036528 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 843 0.30622030425875074 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 793 0.2880577713845662 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 755 0.274254246400186 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 741 0.26916873719541434 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 732 0.2658994812780611 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 724 0.2629934760181916 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 709 0.25754471615593627 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 692 0.2513694549787135 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 675 0.2451941938014908 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 638 0.23175391947459426 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 613 0.222672653037502 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 596 0.21649739186027925 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 588 0.21359138660040974 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 577 0.20959562936808918 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 576 0.20923237871060546 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 569 0.20668962410821962 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 556 0.20196736556093164 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 546 0.19833485898609476 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 531 0.19288609912383942 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 523 0.18998009386396988 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 491 0.1783560728244918 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 486 0.17653981953707334 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 457 0.16600555047004634 No Hit GTACATGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 420 0.1525652761431498 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 382 0.1387617511587696 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 375 0.13621899655638375 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 366 0.13294974063903056 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 352 0.1278642314342589 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 342 0.12423172485942201 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 333 0.12096246894206879 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 328 0.11914621565465033 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 321 0.1166034610522645 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 307 0.11151795184749283 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 303 0.11006494921755809 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 302 0.10970169856007439 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 302 0.10970169856007439 No Hit ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGC 299 0.10861194658762331 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 297 0.10788544527265594 No Hit GTACATGGGAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCA 288 0.10461618935530273 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.6325065748369007E-4 0.0 24-25 0.0 0.0 0.0 0.0014530026299347603 0.0 26-27 0.0 0.0 0.0 0.00217950394490214 0.0 28-29 0.0 0.0 0.0 0.005630385190997195 0.0 30-31 0.0 0.0 0.0 0.010715894395768855 0.0 32-33 0.0 0.0 0.0 0.016527904915507895 0.0 34-35 0.0 0.0 0.0 0.022339915435246938 0.0 36-37 0.0 0.0 0.0 0.028696801941211512 0.0 38-39 0.0 0.0 0.0 0.03741481772082007 0.0 40-41 0.0 0.0 0.0 0.052308094677651365 0.0 42-43 0.0 0.0 0.0 0.06774624762070819 0.0 44-45 0.0 0.0 0.0 0.10298156139662613 0.0 46-47 0.0 0.0 0.0 0.1838048326867472 0.0 48-49 0.0 0.0 0.0 0.23574967670691482 0.0 50-51 0.0 0.0 0.0 0.29695741249291663 0.0 52-53 0.0 0.0 0.0 0.35707539630646734 0.0 54-55 0.0 0.0 0.0 0.42118913735233865 0.0 56-57 0.0 0.0 0.0 0.4983799020676227 0.0 58-59 0.0 0.0 0.0 0.6148017377911454 0.0 60-61 0.0 0.0 0.0 0.7595571247983959 0.0 62-63 0.0 0.0 0.0 0.9479025907036892 0.0 64-65 0.0 0.0 0.0 1.0590572918936982 0.0 66-67 0.0 0.0 0.0 1.2454048791828312 0.0 68-69 0.0 0.0 0.0 1.4675326562341078 0.0 70-71 0.0 0.0 0.0 1.6181000537610974 0.0 72-73 0.0 0.0 0.0 1.8756447699170335 0.0 74-75 0.0 0.0 0.0 2.0701654969995493 0.0 76-77 0.0 0.0 0.0 2.2152841346642838 0.0 78-79 0.0 0.0 0.0 2.41670662423899 0.0 80-81 0.0 0.0 0.0 2.6124987286226986 0.0 82-83 0.0 0.0 0.0 2.8705883207648606 0.0 84-85 0.0 0.0 0.0 3.2796085610914956 0.0 86-87 0.0 0.0 0.0 3.8513650959708237 0.0 88-89 0.0 0.0 0.0 4.47615622684277 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGTT 35 3.4012883E-6 67.88674 5 CAGTCCG 40 5.3646206E-4 47.52936 9 CATTAGC 40 5.374248E-4 47.512085 3 ATGGGTA 115 1.8189894E-12 45.45461 5 GGGTACC 55 4.9353923E-5 43.208515 7 CATGGGT 230 0.0 41.32237 4 AGTACTC 70 4.285359E-6 40.732048 5 TGGGTAC 60 8.253066E-5 39.6006 6 GCAGTCC 50 0.001608787 38.02349 8 ATTAGCA 50 0.0016102251 38.01658 4 GTATTAT 50 0.0016116641 38.00967 1 TACCTGG 120 3.76167E-9 35.634064 2 GTATATG 55 0.0025730177 34.554245 1 ATTATCA 55 0.0025730177 34.554245 3 TAGCAGT 70 2.0440436E-4 33.94337 6 CATGTAG 60 0.003933855 31.686243 8 GCATTAG 60 0.003940865 31.674723 2 TATTATC 60 0.003940865 31.674723 2 GGCATTA 60 0.003940865 31.674723 1 TGCTCCG 30 0.0039477744 31.668968 20-21 >>END_MODULE