##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936697_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 275292 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.778584194237393 31.0 31.0 34.0 30.0 34.0 2 31.808548740973222 31.0 31.0 34.0 30.0 34.0 3 31.844176365459223 31.0 31.0 34.0 30.0 34.0 4 35.65600889237609 37.0 35.0 37.0 33.0 37.0 5 35.47801243770251 37.0 35.0 37.0 33.0 37.0 6 35.71362407915959 37.0 35.0 37.0 33.0 37.0 7 35.66131961698851 37.0 35.0 37.0 33.0 37.0 8 35.69857097191346 37.0 35.0 37.0 33.0 37.0 9 37.20925780625663 39.0 37.0 39.0 33.0 39.0 10-11 37.08663528180986 39.0 37.0 39.0 33.0 39.0 12-13 36.96348604390974 39.0 37.0 39.0 33.0 39.0 14-15 37.62673088938291 39.0 36.5 41.0 32.0 41.0 16-17 37.69405213373436 39.0 36.5 41.0 32.5 41.0 18-19 38.139531842552636 40.0 37.0 41.0 33.5 41.0 20-21 38.28859356610435 40.0 37.5 41.0 34.0 41.0 22-23 38.17005216279441 40.0 37.0 41.0 33.5 41.0 24-25 37.95766131961699 40.0 36.5 41.0 33.0 41.0 26-27 37.53464866396408 39.5 36.0 41.0 32.5 41.0 28-29 37.289705113116256 39.0 35.0 41.0 32.5 41.0 30-31 36.91798345030004 38.5 35.0 40.0 32.0 41.0 32-33 36.35336660709356 38.0 35.0 40.0 31.0 41.0 34-35 35.94387958967206 38.0 35.0 40.0 31.0 41.0 36-37 35.417460369353265 37.5 35.0 40.0 30.0 41.0 38-39 35.017159961059534 37.0 35.0 40.0 28.5 41.0 40-41 34.59007708178952 37.0 34.0 40.0 26.0 41.0 42-43 33.98330136727547 37.0 33.0 40.0 23.0 41.0 44-45 33.17098208447757 36.5 33.0 40.0 16.5 41.0 46-47 32.005621303924556 36.0 32.5 40.0 2.0 41.0 48-49 31.028627784316292 36.0 31.0 40.0 2.0 41.0 50-51 30.488704720805544 35.0 30.0 40.0 2.0 41.0 52-53 30.152706943899567 35.0 29.0 39.0 2.0 41.0 54-55 29.91792351394156 35.0 28.0 39.0 2.0 41.0 56-57 29.567388445723086 35.0 27.5 39.0 2.0 40.5 58-59 29.26336217543554 35.0 27.0 39.0 2.0 40.0 60-61 28.750355985644333 35.0 26.0 38.5 2.0 40.0 62-63 28.297246196765617 35.0 26.0 38.0 2.0 40.0 64-65 27.795825886694857 34.0 24.0 37.5 2.0 39.5 66-67 27.223838687648026 34.0 22.0 36.5 2.0 39.0 68-69 26.97797248013019 34.0 22.0 36.0 2.0 39.0 70-71 26.46182598840504 33.5 20.5 35.5 2.0 38.5 72-73 25.953794516368074 33.0 19.0 35.0 2.0 37.5 74-75 25.423357743777515 33.0 13.5 35.0 2.0 37.0 76-77 23.993290760356278 30.5 7.5 34.0 2.0 35.5 78-79 24.603622698807087 32.0 4.0 35.0 2.0 36.0 80-81 24.305580983101578 32.0 2.0 35.0 2.0 36.0 82-83 23.912690888220506 32.0 2.0 35.0 2.0 35.0 84-85 23.45902350958255 31.5 2.0 35.0 2.0 35.0 86-87 23.030869040872965 31.0 2.0 34.0 2.0 35.0 88-89 22.447335919678014 31.0 2.0 34.0 2.0 35.0 90-91 21.807231957339845 30.0 2.0 34.0 2.0 35.0 92-93 21.33658805922439 30.0 2.0 34.0 2.0 35.0 94-95 20.758937782427385 29.0 2.0 34.0 2.0 35.0 96-97 19.961691585661768 29.0 2.0 34.0 2.0 35.0 98-99 19.154968179242402 27.0 2.0 34.0 2.0 35.0 100-101 16.733039826802084 21.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.45482819247050443 1101 2 0.43323488981540237 1101 3 0.4129831071721135 1101 4 0.21182867240166559 1101 5 0.14351702106749542 1101 6 0.27008871026973225 1101 7 0.262513050244884 1101 8 0.23071262106214618 1101 9 0.4358007820443248 1101 10-11 0.39109770376595776 1101 12-13 0.5597174005020662 1101 14-15 0.5246742957244734 1101 16-17 0.6951342156681832 1101 18-19 0.8047319635931771 1101 20-21 0.7715162642492643 1101 22-23 0.4367906947444169 1101 24-25 0.43260343154629055 1101 26-27 0.3557796980848025 1101 28-29 0.5915909180784169 1101 30-31 0.8280565456802194 1101 32-33 0.9560774825297358 1101 34-35 1.041054418793081 1101 36-37 1.1199028812890433 1101 38-39 0.8571877779906387 1101 40-41 0.6240667760748835 1101 42-43 0.6906073727813364 1101 44-45 0.3689969015377059 1101 46-47 0.3538629946342553 1101 48-49 0.5340137254295882 1101 50-51 0.6647414977754842 1101 52-53 0.6067096283122773 1101 54-55 0.6351457038331567 1101 56-57 0.3273609741658099 1101 58-59 0.4988489319038081 1101 60-61 0.43002974924381476 1101 62-63 0.8035251865780637 1101 64-65 0.891850707223437 1101 66-67 0.7867915822304745 1101 68-69 0.42429444068286415 1101 70-71 0.5998598476152672 1101 72-73 0.4115446360449795 1101 74-75 0.15582366883152687 1101 76-77 -0.16323057476451552 1101 78-79 -0.46161684434736117 1101 80-81 -0.39874018803994105 1101 82-83 -0.02520849283674309 1101 84-85 -0.18420366622718376 1101 86-87 0.005321850306810205 1101 88-89 -0.0506917532522273 1101 90-91 -0.3119466375455424 1101 92-93 -0.3147714927456704 1101 94-95 0.06449424171330875 1101 96-97 -0.014333737756420106 1101 98-99 0.427143647530535 1101 100-101 0.5524628694124232 1102 1 0.1591982084194683 1102 2 0.23585051979944893 1102 3 0.2773531231210775 1102 4 0.25176487654839974 1102 5 0.037105537813907574 1102 6 0.004315185548840361 1102 7 -0.10724771530535548 1102 8 0.10669348230616293 1102 9 0.0025631424908922895 1102 10-11 -0.05939671409687719 1102 12-13 -0.011797751013084223 1102 14-15 0.02901241374680552 1102 16-17 -0.13950693265717717 1102 18-19 0.034907402189666925 1102 20-21 -0.04931308184324479 1102 22-23 -0.177084903341715 1102 24-25 0.12226850331662575 1102 26-27 -0.23369398612572212 1102 28-29 0.1988636453511461 1102 30-31 0.1311506445637889 1102 32-33 0.22625292112623185 1102 34-35 0.41062379678351135 1102 36-37 0.3304610950051554 1102 38-39 0.18621489122827484 1102 40-41 0.3991544953731321 1102 42-43 0.7283745179168974 1102 44-45 0.4328246527338777 1102 46-47 0.3048677793232457 1102 48-49 -0.1592239141238423 1102 50-51 -0.07538609393105489 1102 52-53 0.03940500311291473 1102 54-55 0.20095112648068536 1102 56-57 0.16984901244331851 1102 58-59 -0.3063185322110229 1102 60-61 -0.08209146287739699 1102 62-63 -0.20818135249690073 1102 64-65 0.13622072317239642 1102 66-67 -0.054069661788663126 1102 68-69 -0.5178746183282996 1102 70-71 -0.18785945063034504 1102 72-73 -0.06131022838883027 1102 74-75 -0.036457032922857735 1102 76-77 -0.506547959134533 1102 78-79 -0.1813297630076498 1102 80-81 -0.1344658658708866 1102 82-83 -0.2561813795991057 1102 84-85 -0.26098197563706904 1102 86-87 -0.17726188175060642 1102 88-89 -0.5311702221517471 1102 90-91 -0.5877361112297557 1102 92-93 -0.09952428381425094 1102 94-95 -0.16029044728191266 1102 96-97 -0.3144613294629579 1102 98-99 -0.5127287607629256 1102 100-101 0.3544724387904168 1103 1 0.1318126714062018 1103 2 0.10673504819360957 1103 3 0.12313831781512974 1103 4 -0.0646236557579769 1103 5 0.13099880757367544 1103 6 0.06557809892984778 1103 7 -0.16695796654320816 1103 8 0.15331002365954305 1103 9 0.05333641714093318 1103 10-11 0.13022979119073597 1103 12-13 0.24104777744219774 1103 14-15 0.03472180918662815 1103 16-17 0.034342324632383736 1103 18-19 -0.2920624614939271 1103 20-21 0.14774052779059588 1103 22-23 -0.008315418654383677 1103 24-25 0.03108933588585927 1103 26-27 0.1979399768304475 1103 28-29 0.31558838402710165 1103 30-31 0.11850317222440054 1103 32-33 0.12471859751231307 1103 34-35 0.38562838141582745 1103 36-37 0.4035645854385521 1103 38-39 0.4025789721623241 1103 40-41 0.36650580046513426 1103 42-43 0.866622577089224 1103 44-45 1.0471470440780948 1103 46-47 0.5428652119291542 1103 48-49 0.11140998770389743 1103 50-51 0.3224988623621172 1103 52-53 0.03312711326634599 1103 54-55 0.08847481374763078 1103 56-57 -0.11399474328747061 1103 58-59 0.07039153564153366 1103 60-61 0.10513269507847056 1103 62-63 -0.5571146613788613 1103 64-65 -0.4782728277781487 1103 66-67 0.03705449005435568 1103 68-69 -0.6029592812050062 1103 70-71 -0.5541471210258493 1103 72-73 -0.16516743237440323 1103 74-75 -0.3335839966736991 1103 76-77 -0.4768701059650233 1103 78-79 -0.5537486150157171 1103 80-81 -0.05385580413117097 1103 82-83 0.07516527973215048 1103 84-85 0.6831894284829829 1103 86-87 0.44349733336034447 1103 88-89 0.038772928407574625 1103 90-91 -0.2569640591483342 1103 92-93 -0.37674171764272657 1103 94-95 0.03043025691761514 1103 96-97 -0.23399797595724436 1103 98-99 0.44060579513902454 1103 100-101 1.3147878614935138 1104 1 0.14512456276687402 1104 2 0.1198682564487683 1104 3 -0.06126264707364015 1104 4 0.2511093916823839 1104 5 0.15181538936586492 1104 6 0.18565314583416637 1104 7 0.0019869097187452667 1104 8 0.06906093941046265 1104 9 0.01929728554082999 1104 10-11 -0.07235217968392504 1104 12-13 -0.12315306236839518 1104 14-15 -0.023484212434034646 1104 16-17 0.3664695470035113 1104 18-19 0.1925707914320114 1104 20-21 0.06853924127224076 1104 22-23 0.07888193683565703 1104 24-25 0.09666603560889797 1104 26-27 0.0491862914914023 1104 28-29 0.1256035720910731 1104 30-31 0.02777682540050108 1104 32-33 0.38318370963596493 1104 34-35 0.5426461603848196 1104 36-37 0.6990171004032675 1104 38-39 0.609002629805488 1104 40-41 1.0043598163679235 1104 42-43 0.5120259541999204 1104 44-45 0.7556702282110308 1104 46-47 1.0270964278676828 1104 48-49 1.1172171886330489 1104 50-51 1.2207388337728204 1104 52-53 0.9965864181890645 1104 54-55 0.7895379782254324 1104 56-57 0.7789294057342389 1104 58-59 0.8101842632391403 1104 60-61 1.119740038954106 1104 62-63 0.9231855262383988 1104 64-65 1.1642716196443494 1104 66-67 0.8986197540586467 1104 68-69 1.0444542808427038 1104 70-71 1.2690906168460323 1104 72-73 1.516865981366326 1104 74-75 1.5868126798205395 1104 76-77 1.0242019926680506 1104 78-79 1.3857224829919694 1104 80-81 1.8021256128258578 1104 82-83 1.6373023296740783 1104 84-85 1.0197390577155403 1104 86-87 0.84307742806239 1104 88-89 1.3206901981297214 1104 90-91 1.5108372452383385 1104 92-93 1.46592780795363 1104 94-95 1.4608778580969215 1104 96-97 2.0754364920138073 1104 98-99 1.6351023554892414 1104 100-101 1.2641312010807582 1105 1 0.18605234737696819 1105 2 0.29081283533558633 1105 3 0.19807007796305598 1105 4 0.22365669996976578 1105 5 0.22198108408029782 1105 6 0.32442412215026906 1105 7 0.18353700823634966 1105 8 0.16981337590296874 1105 9 0.22535115946473638 1105 10-11 0.29731365388082764 1105 12-13 0.33561500470292316 1105 14-15 0.6551698651370188 1105 16-17 0.7464896472402174 1105 18-19 0.7961399058314456 1105 20-21 0.49683296920495224 1105 22-23 0.32382712401911107 1105 24-25 0.11567530552068916 1105 26-27 0.30957031180321337 1105 28-29 0.09597555870855246 1105 30-31 0.2810398079053513 1105 32-33 0.3058969771145712 1105 34-35 0.5340705001846118 1105 36-37 0.30366395775769917 1105 38-39 0.32922584822365053 1105 40-41 -0.3319621047915362 1105 42-43 -0.43844415330990216 1105 44-45 -0.3731035251114534 1105 46-47 -0.8710204973250519 1105 48-49 0.07114533028682501 1105 50-51 0.6709246287239559 1105 52-53 0.41018845994995345 1105 54-55 0.5678204658942008 1105 56-57 0.2505444333059472 1105 58-59 -0.16178119609093855 1105 60-61 -0.08365584314213592 1105 62-63 0.22891409369982085 1105 64-65 0.5656019379421728 1105 66-67 0.4148454056633568 1105 68-69 0.5278914213195556 1105 70-71 0.3662858403950473 1105 72-73 0.2012459816308052 1105 74-75 0.21661789265824183 1105 76-77 0.28381241834345516 1105 78-79 0.14371298827489198 1105 80-81 -0.015477962900462217 1105 82-83 0.21173932468868983 1105 84-85 0.19061090548269632 1105 86-87 0.15800317620113447 1105 88-89 0.5760689950247659 1105 90-91 0.8807609436818922 1105 92-93 0.7623732397781247 1105 94-95 1.060116164916458 1105 96-97 1.2924992126866357 1105 98-99 1.0452926465459598 1105 100-101 1.042407076829555 1106 1 0.428661903243718 1106 2 0.16863443688754742 1106 3 0.2898773161659669 1106 4 0.250366362469606 1106 5 0.06594989068897661 1106 6 0.3702763518096859 1106 7 0.08233834950078744 1106 8 0.07552659712167298 1106 9 0.08746043911323653 1106 10-11 0.5052096416501399 1106 12-13 0.2617491054519192 1106 14-15 0.6134352145209832 1106 16-17 0.45732768056627293 1106 18-19 0.19569281768570335 1106 20-21 0.24033541662713276 1106 22-23 0.42300874715463266 1106 24-25 0.31385868874460954 1106 26-27 0.20489001836950393 1106 28-29 0.27537610086716313 1106 30-31 0.2032490400186191 1106 32-33 0.11337285619521964 1106 34-35 0.2214685242605654 1106 36-37 0.15444186543657423 1106 38-39 0.37414022056585594 1106 40-41 0.48719844867275697 1106 42-43 0.4691062404616986 1106 44-45 0.2206500882086857 1106 46-47 0.564260924106911 1106 48-49 0.4036093255589961 1106 50-51 -0.07142155625233926 1106 52-53 0.21415058593116854 1106 54-55 0.379105587365995 1106 56-57 0.28524515404287243 1106 58-59 0.32452994128588486 1106 60-61 0.7169595454262812 1106 62-63 0.15405252400931602 1106 64-65 -0.017803126487340393 1106 66-67 0.15124968640387948 1106 68-69 -0.14122480875097665 1106 70-71 0.18313386896757677 1106 72-73 -0.0021855483613606452 1106 74-75 0.08649982541515655 1106 76-77 0.13923464684661369 1106 78-79 -0.032687695204689504 1106 80-81 -0.03578321618617508 1106 82-83 -0.030889502218819587 1106 84-85 -0.12914705024530093 1106 86-87 -0.16770953112314757 1106 88-89 -0.21140996742240148 1106 90-91 0.21880069341530728 1106 92-93 0.6535817909812778 1106 94-95 0.6761021355987786 1106 96-97 0.521175806080528 1106 98-99 0.05412520689643685 1106 100-101 0.43197758956771537 1107 1 0.14878756642987767 1107 2 0.2553993919799069 1107 3 0.3746347888237942 1107 4 -0.1518210112480176 1107 5 0.19210842965890151 1107 6 0.24059820077921756 1107 7 0.26206016979200086 1107 8 0.16063603098555035 1107 9 0.3526306188741586 1107 10-11 0.34156723423549096 1107 12-13 0.15706671785138582 1107 14-15 0.31534362639380475 1107 16-17 0.3829530634870295 1107 18-19 0.2237063225675442 1107 20-21 0.09601176874476636 1107 22-23 0.21990757786129933 1107 24-25 -0.19087975193689033 1107 26-27 0.2176844599895631 1107 28-29 0.09813104461854749 1107 30-31 -0.23962244199876892 1107 32-33 0.015051841504103436 1107 34-35 0.01517363291229401 1107 36-37 -0.028089126702958822 1107 38-39 0.09251911332197693 1107 40-41 0.1984990105071205 1107 42-43 -0.31947587730191884 1107 44-45 -0.2809598084190057 1107 46-47 -0.32638342561216405 1107 48-49 -0.08607951466365193 1107 50-51 0.015610628644616042 1107 52-53 0.0057439273465753615 1107 54-55 -0.04379535510790333 1107 56-57 -0.1954295686247356 1107 58-59 0.0024919555468336796 1107 60-61 -0.1018716826576167 1107 62-63 0.6612807643336396 1107 64-65 0.5030994584721888 1107 66-67 0.2063120617509533 1107 68-69 0.31551655190497385 1107 70-71 0.2452810930365139 1107 72-73 -0.06188859738825414 1107 74-75 -0.31062321761535827 1107 76-77 -0.38239141392535814 1107 78-79 -0.15823356096407792 1107 80-81 -0.058680247980003486 1107 82-83 0.0860202783920272 1107 84-85 -0.01139647341998895 1107 86-87 0.293626878611839 1107 88-89 0.003840381279903937 1107 90-91 0.06211929652038961 1107 92-93 -0.02125167922585902 1107 94-95 0.07443097165003465 1107 96-97 0.1377075542848729 1107 98-99 0.6094613298482194 1107 100-101 0.9198088567584151 1108 1 0.10022235550304259 1108 2 0.22992056576306652 1108 3 0.1106281927138788 1108 4 -0.181354823069654 1108 5 0.2746273867166096 1108 6 0.1959122594143139 1108 7 0.06233861213686964 1108 8 -0.13925718772685514 1108 9 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90-91 0.533791067372487 2316 92-93 0.7181346175374905 2316 94-95 0.009360113546389215 2316 96-97 -0.20239633090098152 2316 98-99 -0.33421998883310167 2316 100-101 0.05932576061957917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 8.0 5 20.0 6 66.0 7 114.0 8 256.0 9 511.0 10 847.0 11 1409.0 12 2350.0 13 3148.0 14 3976.0 15 5699.0 16 7968.0 17 10423.0 18 8124.0 19 3920.0 20 3136.0 21 3285.0 22 3622.0 23 3947.0 24 4327.0 25 4641.0 26 5042.0 27 6050.0 28 6970.0 29 7792.0 30 9043.0 31 10590.0 32 12729.0 33 15659.0 34 20388.0 35 27468.0 36 31359.0 37 33073.0 38 16451.0 39 880.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.11338273076461 18.719906389566216 14.972945276416057 13.193765603253107 2 15.514948959203693 26.655647182766014 36.666908853126024 21.162495004904276 3 24.714741878180888 32.64130312376261 30.31964167002692 12.324313328029584 4 11.15010043990134 18.489659957353908 37.74578715459027 32.61445244815449 5 9.707147319936649 44.363802798483064 29.765485375528527 16.163564506051756 6 23.149601150778082 38.769016171919276 16.939467910436917 21.141914766865728 7 22.31339813725063 41.80215916190808 18.944611539746887 16.9398311610944 8 27.328436714470456 27.5612803859175 18.678348807811343 26.431934091800706 9 28.639461218796313 19.614367498764928 21.79426636831246 29.9519049141263 10-11 24.90782481083653 28.62516391745462 22.327282766236458 24.13972850547239 12-13 33.0922537910091 27.881012534077758 23.77733258987357 15.249401085039569 14-15 26.89922734059132 26.698888053385062 32.31456355822917 14.087321047794452 16-17 16.66993602946786 30.74763062012547 32.04375133953059 20.53868201087608 18-19 15.840089795562529 28.406201415224565 39.47699170335498 16.276717085857925 20-21 20.011478720776484 23.668504714993535 38.373981081905754 17.946035482324223 22-23 18.01957575570445 24.768471009609883 38.62715432299452 18.58479891169115 24-25 19.43760818196183 25.199200111882625 39.395397918910525 15.967793787245018 26-27 19.411543540292683 22.62845580582644 42.73166000374165 15.228340650139222 28-29 16.348778791087636 26.589605874735046 41.819344268845775 15.242271065331545 30-31 16.751486619516218 26.68413921369906 40.142541111341174 16.421833055443553 32-33 16.30710775143394 27.020498309062045 39.915398614556956 16.756995324947056 34-35 18.390355720886976 24.99128135080938 39.810442035513965 16.807920892789678 36-37 18.452515021833428 25.057580667427143 41.02722457550151 15.462679735237916 38-39 18.764270054364708 25.88108787538076 40.4482136661938 14.906428404060733 40-41 16.69621126811726 27.59163064404809 39.978386428856844 15.733771658977805 42-43 15.493604948944933 29.6745988790044 36.766548851942126 18.06524732010854 44-45 17.359306179902827 27.831812196340188 35.50742405666803 19.301457567088953 46-47 18.62571129148557 29.342714904019925 33.705181598414775 18.326392206079735 48-49 18.84739729013041 28.972901304079336 32.82683715354717 19.35286425224309 50-51 17.55333930280668 30.63792843533807 33.74211511881129 18.06661714304396 52-53 17.594684723301924 32.312138098386384 30.741748461577583 19.351428716734112 54-55 15.730300344371631 30.276914023335898 31.831853649322156 22.160931982970315 56-57 19.091893187836696 30.58753228448855 28.904016535470255 21.4165579922045 58-59 20.70177414198948 29.162731394030978 29.278246781552408 20.85724768242713 60-61 19.119519473702372 29.609690444145357 32.266788723542994 19.00400135860928 62-63 20.81271751055312 29.487892063907235 31.072131766894078 18.62725865864557 64-65 19.732250710698164 31.996040071569347 30.928675876224986 17.343033341507496 66-67 19.70985747161511 30.08317031660664 29.997620591510056 20.2093516202682 68-69 18.95636979869551 31.37647372942773 30.23381690245732 19.433339569419445 70-71 20.51476250672014 31.347260363541256 28.516811240428346 19.62116588931026 72-73 19.29832846263243 28.996805135032538 31.051036106151614 20.65383029618342 74-75 17.895864931384924 33.10706857874613 29.821537231965273 19.175529257903676 76-77 18.44964028123632 29.762118826159845 30.831557897375262 20.956682995228572 78-79 17.663555306113924 29.574635418653266 32.56847990148267 20.193329373750142 80-81 18.140057833706297 31.226023714725798 30.747951117440202 19.8859673341277 82-83 16.97439092393565 29.832148753875693 31.47142750755158 21.72203281463708 84-85 17.104271299735714 29.555250801493095 30.56953690501058 22.770940993760615 86-87 17.433080677357772 30.02900730359222 30.064971274232544 22.472940744817464 88-89 17.771625995880242 28.57127287391148 29.737265629825 23.919835500383275 90-91 17.26174364973687 27.21598433392856 31.280915694954846 24.24135632137972 92-93 17.408953944345253 29.9271849509511 31.007332361012978 21.656528743690664 94-95 16.643841587898955 29.491575911247374 30.27858704891711 23.585995451936558 96-97 14.563320165281752 28.830767833628478 31.40734686464151 25.19856513644826 98-99 16.99370623654742 28.934782806128474 28.820532381549192 25.250978575774912 100-101 17.045504083234036 27.769987503269494 29.68899851782964 25.495509895666828 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1694.0 1 1243.5 2 1291.5 3 1622.5 4 1257.5 5 1135.5 6 1617.0 7 2832.5 8 3916.0 9 4144.5 10 3430.5 11 2541.5 12 2272.5 13 2167.5 14 2062.0 15 1911.0 16 1691.0 17 1567.5 18 1563.5 19 1542.5 20 1545.5 21 1590.0 22 1600.5 23 1696.5 24 1821.5 25 1918.5 26 2079.5 27 2312.0 28 2604.0 29 2891.0 30 3234.5 31 3587.5 32 3944.5 33 4432.0 34 4987.5 35 5252.0 36 5846.0 37 6514.5 38 6518.5 39 6346.5 40 5954.5 41 5528.0 42 5342.0 43 5251.0 44 5318.0 45 5387.0 46 6141.5 47 12043.0 48 30004.5 49 28872.5 50 11850.5 51 6588.5 52 3557.5 53 2566.0 54 2301.0 55 2193.0 56 2428.5 57 2676.5 58 2745.5 59 2947.0 60 3113.5 61 3198.0 62 2742.5 63 1547.5 64 733.5 65 574.5 66 462.5 67 358.5 68 270.0 69 195.5 70 167.5 71 140.0 72 115.5 73 127.5 74 106.5 75 88.5 76 74.5 77 48.0 78 38.0 79 32.5 80 28.0 81 19.5 82 10.0 83 11.0 84 7.0 85 1.5 86 1.5 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.039594321665722215 2 0.007991514464641181 3 0.0054487598622553505 4 0.00108975197245107 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0014530026299347603 10-11 3.6325065748369007E-4 12-13 9.081266437092251E-4 14-15 0.0032692559173532103 16-17 0.0032692559173532103 18-19 0.0 20-21 0.0 22-23 9.081266437092251E-4 24-25 0.001271377301192915 26-27 0.004540633218546126 28-29 0.0041773825610624355 30-31 0.00108975197245107 32-33 3.6325065748369007E-4 34-35 0.007265013149673801 36-37 0.009444517094575942 38-39 0.007809889135899336 40-41 7.265013149673801E-4 42-43 3.6325065748369007E-4 44-45 0.0016346279586766052 46-47 1.8162532874184504E-4 48-49 7.265013149673801E-4 50-51 9.081266437092251E-4 52-53 0.0036325065748369005 54-55 0.0029060052598695206 56-57 0.0018162532874184502 58-59 0.0014530026299347603 60-61 0.0038141319035787454 62-63 0.006538511834706421 64-65 0.012532147683187307 66-67 0.004903883876029816 68-69 0.004540633218546126 70-71 0.0 72-73 0.0023611292736439855 74-75 0.008899641108350405 76-77 0.0027243799311276753 78-79 0.0038141319035787454 80-81 0.007265013149673801 82-83 0.006720137163448266 84-85 0.008899641108350405 86-87 0.005993635848480886 88-89 0.011987271696961772 90-91 0.017254406230475278 92-93 0.0019978786161602953 94-95 0.0036325065748369005 96-97 0.0016346279586766052 98-99 0.007083387820931956 100-101 0.007265013149673801 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 275292.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.83028929282362 #Duplication Level Percentage of deduplicated Percentage of total 1 79.15836679355478 27.57108815366956 2 10.889085884132033 7.585400229574415 3 3.6856651196746104 3.851183470642082 4 1.7927725921677007 2.4977115208578526 5 0.9959847734265006 1.73452188948462 6 0.6069771079939511 1.2684712959330455 7 0.43906763310215363 1.0704996876044346 8 0.33790478176982847 0.9415457041977247 9 0.23048443447880274 0.7225055577350594 >10 1.5320435938885122 9.86879386251689 >50 0.1491369870156959 3.7037037037037033 >100 0.13349324711894459 9.152463565959055 >500 0.019815403869218335 4.835592752422882 >1k 0.02607289982791886 17.72336282928672 >5k 0.003128747979350263 7.473155776411955 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7493 2.7218371765252893 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6585 2.392005579530099 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6495 2.359313020356567 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4403 1.5993926449006872 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3826 1.389797015532598 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3613 1.312424625488572 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3467 1.2593900294959535 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3177 1.1540473388256831 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2671 0.9702425061389361 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2094 0.760646876770847 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2081 0.7559246182235589 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1789 0.6498554262383215 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1763 0.6404109091437455 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1761 0.6396844078287782 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1726 0.626970634816849 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1558 0.5659445243595891 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1532 0.5565000072650131 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1485 0.5394272263632798 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1443 0.5241706987489647 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1416 0.5143629309969051 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1255 0.455879575142031 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1253 0.4551530738270636 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1181 0.42899902648823796 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1171 0.4253665199134011 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1036 0.3763276811531029 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1035 0.37596443049561923 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1032 0.37487467852316814 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1023 0.3716054226058149 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 994 0.3610711535387879 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 935 0.3396393647472502 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 923 0.33528035685744595 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 871 0.316391322668294 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 754 0.2738909957427023 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 733 0.26626273193554484 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 728 0.2644464786481263 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 686 0.2491899510338114 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 680 0.24701044708890924 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 671 0.24374119117155602 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 668 0.24265143919910498 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 645 0.23429667407698007 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 614 0.22303590369498566 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 612 0.22230940238001834 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 594 0.21577089054531187 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 593 0.21540763988782818 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 553 0.20087761358848058 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 534 0.19397585109629048 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 524 0.19034334452145357 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 498 0.18089882742687763 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 496 0.18017232611191025 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 488 0.17726632085204075 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 479 0.17399706493468753 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 479 0.17399706493468753 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 474 0.1721808116472691 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 381 0.1383985005012859 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 354 0.12859073274922628 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 347 0.12604797814684043 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 336 0.12205222091451985 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 329 0.11950946631213402 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 313 0.11369745579239499 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 299 0.10861194658762331 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 299 0.10861194658762331 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 294 0.10679569330020487 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 293 0.10643244264272118 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 290 0.1053426906702701 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 287 0.10425293869781906 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 281 0.1020734347529169 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 279 0.10134693343794951 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 277 0.10062043212298215 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 277 0.10062043212298215 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 3.6325065748369007E-4 0.0 24-25 0.0 0.0 0.0 0.0018162532874184502 0.0 26-27 0.0 0.0 0.0 0.002361129273643985 0.0 28-29 0.0 0.0 0.0 0.005630385190997195 0.0 30-31 0.0 0.0 0.0 0.010534269067027011 0.0 32-33 0.0 0.0 0.0 0.016164654258024207 0.0 34-35 0.0 0.0 0.0 0.022339915435246938 0.0 36-37 0.0 0.0 0.0 0.028696801941211512 0.0 38-39 0.0 0.0 0.0 0.037051567063336385 0.0 40-41 0.0 0.0 0.0 0.052852970663876904 0.0 42-43 0.0 0.0 0.0 0.0693808755793848 0.0 44-45 0.0 0.0 0.0 0.1040713133690772 0.0 46-47 0.0 0.0 0.0 0.1838048326867472 0.0 48-49 0.0 0.0 0.0 0.23611292736439854 0.0 50-51 0.0 0.0 0.0 0.29877366578033504 0.0 52-53 0.0 0.0 0.0 0.36125277886752977 0.0 54-55 0.0 0.0 0.0 0.4275460238583032 0.0 56-57 0.0 0.0 0.0 0.5051000392310709 0.0 58-59 0.0 0.0 0.0 0.6242462548857214 0.0 60-61 0.0 0.0 0.0 0.7697281432079393 0.0 62-63 0.0 0.0 0.0 0.9595266117431673 0.0 64-65 0.0 0.0 0.0 1.0717710649056276 0.0 66-67 0.0 0.0 0.0 1.2592084041672116 0.0 68-69 0.0 0.0 0.0 1.485150313122067 0.0 70-71 0.0 0.0 0.0 1.6411664705113116 0.0 72-73 0.0 0.0 0.0 1.904341571858245 0.0 74-75 0.0 0.0 0.0 2.101405053543147 0.0 76-77 0.0 0.0 0.0 2.251427575083911 0.0 78-79 0.0 0.0 0.0 2.459751827150807 0.0 80-81 0.0 0.0 0.0 2.6629905700129317 0.0 82-83 0.0 0.0 0.0 2.9292533019484766 0.0 84-85 0.0 0.0 0.0 3.348262935355913 0.0 86-87 0.0 0.0 0.0 3.9272844833849145 0.0 88-89 0.0 0.0 0.0 4.558795751420311 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGCA 20 1.7424403E-5 94.98928 4 TATCACG 25 5.2711526E-5 75.991425 2 ATCACGC 25 5.2711526E-5 75.991425 3 ATGGGCG 25 0.0050194715 56.99357 5 GATAGTC 35 2.7857625E-4 54.27959 7 GCTTATG 70 7.876042E-8 47.5119 1 CGTCACT 50 2.8171724E-5 47.5119 1 GGGAGTA 70 7.898598E-8 47.49464 7 AGATAGT 55 4.9567345E-5 43.17695 6 GGAACGC 55 4.9567345E-5 43.17695 8 CACGCAG 45 9.617641E-4 42.217457 5 AGGCTTA 80 2.2562199E-7 41.55781 6 TAGATAG 70 4.301568E-6 40.709694 5 TACCTGG 95 1.9210347E-8 39.995487 2 CTTATGT 85 3.6304846E-7 39.113235 2 GACCCCG 25 0.0016145381 38.002617 56-57 GTTCGAC 25 0.0016159799 37.995712 16-17 GGAGTAA 50 0.0016145759 37.995712 8 AGTTCGA 25 0.0016159799 37.995712 14-15 TAGTCAA 50 0.0016145759 37.995712 9 >>END_MODULE