##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936696_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 279776 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80744238247741 31.0 31.0 34.0 30.0 34.0 2 31.855548724694042 31.0 31.0 34.0 30.0 34.0 3 31.85953048152808 31.0 31.0 34.0 30.0 34.0 4 35.63009336040261 37.0 35.0 37.0 33.0 37.0 5 35.51949774104998 37.0 35.0 37.0 33.0 37.0 6 35.723993480498685 37.0 35.0 37.0 33.0 37.0 7 35.68013696671623 37.0 35.0 37.0 33.0 37.0 8 35.71225551870067 37.0 35.0 37.0 33.0 37.0 9 37.211022389340044 39.0 37.0 39.0 33.0 39.0 10-11 37.10066624728354 39.0 37.0 39.0 33.0 39.0 12-13 36.979353125357434 39.0 37.0 39.0 33.0 39.0 14-15 37.66880111231843 39.0 37.0 41.0 32.0 41.0 16-17 37.72180065480956 39.0 36.5 41.0 32.5 41.0 18-19 38.17092245224751 40.0 37.0 41.0 33.5 41.0 20-21 38.30035278222578 40.0 37.5 41.0 34.0 41.0 22-23 38.183157240077776 40.0 37.0 41.0 33.5 41.0 24-25 37.93961776564109 40.0 36.5 41.0 33.0 41.0 26-27 37.55482242937207 39.5 36.0 41.0 32.5 41.0 28-29 37.312217631247854 39.0 35.0 41.0 32.5 41.0 30-31 36.947706379389224 38.5 35.0 40.0 32.0 41.0 32-33 36.389323959167335 38.0 35.0 40.0 31.5 41.0 34-35 35.960291447443666 38.0 35.0 40.0 31.0 41.0 36-37 35.48933432460254 37.5 35.0 40.0 30.0 41.0 38-39 35.0908190838385 37.0 35.0 40.0 29.5 41.0 40-41 34.67939887624385 37.0 34.5 40.0 26.5 41.0 42-43 34.060419764382935 37.0 33.5 40.0 23.5 41.0 44-45 33.22026371096877 36.5 33.0 40.0 17.5 41.0 46-47 32.038151950131535 36.0 32.5 40.0 2.0 41.0 48-49 31.046208395287657 36.0 31.0 40.0 2.0 41.0 50-51 30.558209067253806 35.0 30.0 40.0 2.0 41.0 52-53 30.14181345076061 35.0 29.0 39.0 2.0 41.0 54-55 29.962564694612833 35.0 28.5 39.0 2.0 41.0 56-57 29.62726788573716 35.0 28.0 39.0 2.0 40.5 58-59 29.284298867665562 35.0 27.0 39.0 2.0 40.0 60-61 28.72984816424568 35.0 26.0 38.5 2.0 40.0 62-63 28.366341287315567 35.0 26.0 38.0 2.0 40.0 64-65 27.87638682374471 34.0 24.5 37.5 2.0 39.5 66-67 27.313720619352626 34.0 22.5 36.5 2.0 39.0 68-69 26.968832208624043 34.0 22.0 36.0 2.0 39.0 70-71 26.550145473521674 33.5 21.5 35.5 2.0 38.5 72-73 26.02576346791719 33.0 19.0 35.0 2.0 37.5 74-75 25.534247040489532 33.0 15.5 35.0 2.0 37.0 76-77 24.06355977639254 30.5 9.0 34.0 2.0 35.5 78-79 24.659423967745624 32.0 4.5 35.0 2.0 36.0 80-81 24.344900920736592 32.0 2.0 35.0 2.0 36.0 82-83 23.978627544893058 32.0 2.0 35.0 2.0 35.0 84-85 23.538482571771702 32.0 2.0 35.0 2.0 35.0 86-87 23.138392857142854 31.0 2.0 34.0 2.0 35.0 88-89 22.507809819284 31.0 2.0 34.0 2.0 35.0 90-91 21.846475394601395 30.0 2.0 34.0 2.0 35.0 92-93 21.290024877044495 29.5 2.0 34.0 2.0 35.0 94-95 20.751513710968773 29.0 2.0 34.0 2.0 35.0 96-97 19.999458495367723 29.0 2.0 34.0 2.0 35.0 98-99 19.27939673167105 28.0 2.0 34.0 2.0 35.0 100-101 16.83930001143772 21.5 2.0 31.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2599415655810695 1101 2 0.26235059601469146 1101 3 0.11010898734057761 1101 4 0.1353197602374152 1101 5 -0.18296821530433505 1101 6 -0.08141336262464449 1101 7 0.13543561477213473 1101 8 -0.1822761823398693 1101 9 0.16298073891962872 1101 10-11 0.0871735968666556 1101 12-13 0.08386838919084028 1101 14-15 0.32366286386298526 1101 16-17 0.24181474972139938 1101 18-19 0.28008160881363864 1101 20-21 0.10997743812810512 1101 22-23 0.18650249274723052 1101 24-25 0.27357552324150447 1101 26-27 0.5388632306069496 1101 28-29 0.3528351308361479 1101 30-31 0.3659374686775436 1101 32-33 0.5801661938536924 1101 34-35 0.7248956287767854 1101 36-37 0.9320217308327372 1101 38-39 0.5376415178764589 1101 40-41 0.7523678926509447 1101 42-43 0.7876098138768413 1101 44-45 1.025098535454724 1101 46-47 0.6588517905662812 1101 48-49 0.7232384085352663 1101 50-51 1.054831480289252 1101 52-53 0.9468269673431244 1101 54-55 0.6307198056182877 1101 56-57 0.9854342470108115 1101 58-59 1.0932334898559652 1101 60-61 1.145366405481763 1101 62-63 1.1403341301158996 1101 64-65 1.063232433345565 1101 66-67 0.974641298863375 1101 68-69 1.0676463645671888 1101 70-71 0.5912206888083062 1101 72-73 0.9163067344003117 1101 74-75 0.9541354433600056 1101 76-77 0.8279416790654928 1101 78-79 0.9435159023148998 1101 80-81 0.928325612484393 1101 82-83 1.2210743141413332 1101 84-85 1.3106864767084545 1101 86-87 0.9088241277000684 1101 88-89 1.2070616879486913 1101 90-91 0.6057308956472909 1101 92-93 0.8413757941298492 1101 94-95 0.630501938473401 1101 96-97 0.06140121247994301 1101 98-99 -0.3293617192996656 1101 100-101 1.0123303497776348 1102 1 -0.03876963704227876 1102 2 0.012789092163551175 1102 3 -0.00306114916518041 1102 4 0.2405969660636984 1102 5 0.25655744625289856 1102 6 0.23102467596302034 1102 7 0.19396531811995743 1102 8 0.15601406398057804 1102 9 0.2546073689813966 1102 10-11 0.15962078667828905 1102 12-13 -0.03402987970262217 1102 14-15 0.09482506930795864 1102 16-17 0.06285112583670838 1102 18-19 0.005468629327630481 1102 20-21 -0.02056095888249132 1102 22-23 0.20743591759281088 1102 24-25 0.2980169172779483 1102 26-27 0.17986834004032914 1102 28-29 0.16353824987832155 1102 30-31 0.337797454950703 1102 32-33 -0.011574826507775526 1102 34-35 -0.546323771818038 1102 36-37 -0.5386752873934526 1102 38-39 -0.47427413834255816 1102 40-41 -0.4296270741758619 1102 42-43 -0.4289119584101968 1102 44-45 -0.30015121669962497 1102 46-47 -0.4371598009840909 1102 48-49 -0.8305250323249496 1102 50-51 -0.9738423548361936 1102 52-53 -0.7997794537656979 1102 54-55 -0.9778175237495148 1102 56-57 -1.08232294051302 1102 58-59 -0.7381552083824232 1102 60-61 -0.8628696960968227 1102 62-63 -1.1404749906039946 1102 64-65 -0.7594054069655769 1102 66-67 -0.9706992029287385 1102 68-69 -0.9852210189453601 1102 70-71 -1.519528186353746 1102 72-73 -1.1074483178379246 1102 74-75 -1.508001367438439 1102 76-77 -1.2929688677201412 1102 78-79 -1.5445112463162296 1102 80-81 -1.3343335806826921 1102 82-83 -1.2426639608529513 1102 84-85 -1.35365425386426 1102 86-87 -1.546754305915428 1102 88-89 -1.9641233955562285 1102 90-91 -1.7915275501698353 1102 92-93 -1.481872127779706 1102 94-95 -1.4864049478226384 1102 96-97 -1.2081023336193475 1102 98-99 -1.1844673396763916 1102 100-101 -0.276810197770601 1103 1 0.2024408321533535 1103 2 0.42515879509885934 1103 3 0.45550880111371583 1103 4 0.15118730956840665 1103 5 0.14549260690002797 1103 6 0.19167685536283585 1103 7 0.09392998332275226 1103 8 0.15911847830631132 1103 9 0.27054588643426314 1103 10-11 0.1943624640715882 1103 12-13 0.06726741213756071 1103 14-15 0.2613087522033908 1103 16-17 0.17605498237742978 1103 18-19 -0.00981367046288284 1103 20-21 -0.30060815607590285 1103 22-23 -0.027843353500806245 1103 24-25 0.04081745234773848 1103 26-27 -0.2179257590485335 1103 28-29 -0.11782138263979647 1103 30-31 -0.0384171787031633 1103 32-33 -0.03518604278198012 1103 34-35 0.4052423168722967 1103 36-37 0.2991947815094065 1103 38-39 0.4051143172744105 1103 40-41 0.9876642612270743 1103 42-43 0.8651682011011701 1103 44-45 0.6621793655673542 1103 46-47 0.6841773686986556 1103 48-49 -0.4669425886713299 1103 50-51 -0.6215291875764066 1103 52-53 -0.36822169399183835 1103 54-55 -0.4033431288579159 1103 56-57 -0.44898455935151205 1103 58-59 -0.38411315997375084 1103 60-61 -0.46334671585907117 1103 62-63 0.1037073874278569 1103 64-65 -0.06899501853520107 1103 66-67 -0.21891984349813143 1103 68-69 0.04829025033103562 1103 70-71 -0.1922459370415126 1103 72-73 0.06648722139631857 1103 74-75 0.2855172211888046 1103 76-77 0.28619369758613544 1103 78-79 0.06421897250712405 1103 80-81 0.10846821634413573 1103 82-83 0.08341311948323593 1103 84-85 0.4154454659812785 1103 86-87 0.770097868436558 1103 88-89 0.5302545858172856 1103 90-91 0.7776718642820981 1103 92-93 0.823212297146469 1103 94-95 0.7883483395754567 1103 96-97 1.4350666929069895 1103 98-99 1.8654430613735578 1103 100-101 0.9867369110858135 1104 1 0.09488162243436804 1104 2 0.2231094638925768 1104 3 0.307748339707409 1104 4 0.2888483722754813 1104 5 -0.08939510938417783 1104 6 0.01879674737412529 1104 7 0.07975646509965628 1104 8 0.01125984534521507 1104 9 0.13450465438125292 1104 10-11 0.20927840467780356 1104 12-13 0.10143111320517306 1104 14-15 0.002565348104731413 1104 16-17 0.4973950241003422 1104 18-19 0.18600176701411186 1104 20-21 0.03649455591330053 1104 22-23 -0.2118475257918817 1104 24-25 -0.013416195952672183 1104 26-27 0.13587225297747807 1104 28-29 0.029043263329036506 1104 30-31 0.011006063412438039 1104 32-33 0.04731487757634767 1104 34-35 0.017053582058217387 1104 36-37 0.118111212973389 1104 38-39 -0.13415430464238653 1104 40-41 0.1253007810454605 1104 42-43 0.225942529239596 1104 44-45 0.6815068904170261 1104 46-47 0.8601989664407057 1104 48-49 1.1390151975601128 1104 50-51 1.439950748612084 1104 52-53 1.4359383256424714 1104 54-55 1.1138430289528536 1104 56-57 0.7771781600005525 1104 58-59 1.015684361194488 1104 60-61 1.1504832019222526 1104 62-63 1.1615181617559962 1104 64-65 1.1645857889258373 1104 66-67 1.0086111418988466 1104 68-69 0.8188019695603899 1104 70-71 0.808987343115561 1104 72-73 0.8149410194083444 1104 74-75 0.5512062627890018 1104 76-77 0.7330399363884403 1104 78-79 0.26091795397724127 1104 80-81 0.19698214443346984 1104 82-83 0.5227799129548281 1104 84-85 0.49828998678137637 1104 86-87 0.3643364253147041 1104 88-89 0.3035948729690823 1104 90-91 0.12895423021656427 1104 92-93 0.13250791624083647 1104 94-95 -0.12311318944650651 1104 96-97 -0.13859878752005983 1104 98-99 0.14760651701228866 1104 100-101 0.3250729032320905 1105 1 -0.05946130919260284 1105 2 0.016627653251507724 1105 3 0.1937656403354424 1105 4 0.055432090725538785 1105 5 0.166808232243973 1105 6 0.18810590722718246 1105 7 -0.09473103341912292 1105 8 0.06030594085415686 1105 9 -0.12445967788560353 1105 10-11 0.24944311613650427 1105 12-13 -0.008878165145340233 1105 14-15 -0.0565056318692001 1105 16-17 0.2766224207113197 1105 18-19 0.16749246496410564 1105 20-21 0.1778560916402867 1105 22-23 0.24874614644416937 1105 24-25 0.19821432899104252 1105 26-27 0.20571465136590916 1105 28-29 0.0589283208003053 1105 30-31 0.09019198591509792 1105 32-33 0.12145873348345049 1105 34-35 0.27322729932328826 1105 36-37 0.34457761894565664 1105 38-39 0.26808529720617 1105 40-41 0.715287746325707 1105 42-43 0.7833604697538732 1105 44-45 0.9825496679973114 1105 46-47 0.3536934563091805 1105 48-49 -0.26523293610501497 1105 50-51 0.2426524905246339 1105 52-53 0.11633884703126185 1105 54-55 -0.20065513433663185 1105 56-57 -0.10882729088225318 1105 58-59 -0.12087802771414857 1105 60-61 0.16326907702594085 1105 62-63 0.053555132961111696 1105 64-65 0.0600177121394907 1105 66-67 0.04218739500940316 1105 68-69 0.7097961631852243 1105 70-71 0.6309982448811731 1105 72-73 0.48058268311257635 1105 74-75 0.25554326954335593 1105 76-77 0.17197227434317952 1105 78-79 -0.34512541609030123 1105 80-81 -0.5417072737440378 1105 82-83 -0.3548596177952632 1105 84-85 -0.5479951180888669 1105 86-87 -0.7076448520878351 1105 88-89 -1.023511419245633 1105 90-91 -1.0527142139118837 1105 92-93 -1.7569872846123467 1105 94-95 -1.7951714901930416 1105 96-97 -1.6052654541867248 1105 98-99 -1.2092900347118523 1105 100-101 -0.9177402262856269 1106 1 0.18108851898609046 1106 2 0.09594693594153725 1106 3 -0.02503970313124171 1106 4 0.320341840558406 1106 5 0.23710836242940303 1106 6 0.05226738406942388 1106 7 -0.09542301000920617 1106 8 -0.06103625916225752 1106 9 0.3833924971492877 1106 10-11 0.008130940867047798 1106 12-13 0.36832531115739897 1106 14-15 -0.37992258881418195 1106 16-17 0.013659588064093953 1106 18-19 0.2929689318958353 1106 20-21 0.10444206679949986 1106 22-23 -0.0050274716551328424 1106 24-25 0.08130547051785442 1106 26-27 0.040214962168960255 1106 28-29 -0.11399545621903684 1106 30-31 0.21963852192720168 1106 32-33 0.33307455746336956 1106 34-35 0.19329813896064962 1106 36-37 -0.20883359107509847 1106 38-39 0.31688723943199193 1106 40-41 0.398538394320731 1106 42-43 0.726372092614902 1106 44-45 0.03410192395828204 1106 46-47 -0.09263144429248982 1106 48-49 -0.28729408807011225 1106 50-51 0.010799283389061998 1106 52-53 -0.31153725397386367 1106 54-55 -0.3539279490393774 1106 56-57 -0.5487015622543616 1106 58-59 -0.6301141559291992 1106 60-61 -0.16807628966300925 1106 62-63 -0.34275393427790135 1106 64-65 -0.5462945217720545 1106 66-67 -0.16097224047139846 1106 68-69 -0.391648777762196 1106 70-71 0.04828944974142857 1106 72-73 0.09686993549637535 1106 74-75 -0.03109337237635401 1106 76-77 0.1039885064720032 1106 78-79 -0.05333413499533535 1106 80-81 0.17692800767932226 1106 82-83 0.05117817116360612 1106 84-85 0.34843709752634666 1106 86-87 0.42461063974332447 1106 88-89 0.33921332654093206 1106 90-91 0.25319808098742413 1106 92-93 0.4054454235963725 1106 94-95 0.9922216346090416 1106 96-97 1.2815377312512766 1106 98-99 1.2446348799639146 1106 100-101 1.4446092101639465 1107 1 0.15695058795160932 1107 2 0.2575922225132672 1107 3 0.07671385694878552 1107 4 0.20608975158582865 1107 5 0.11060489061581791 1107 6 0.01534847151205554 1107 7 0.03837715475482639 1107 8 0.0836736384486585 1107 9 -0.23790913872220187 1107 10-11 0.06617495640194448 1107 12-13 -0.18305164541551022 1107 14-15 -0.11295189327457678 1107 16-17 0.01636054134172582 1107 18-19 0.027381077358931805 1107 20-21 -0.07040199581083328 1107 22-23 0.13642833627708484 1107 24-25 -0.29272654078025084 1107 26-27 -0.1020587815052778 1107 28-29 -0.005439495291646779 1107 30-31 -0.4545111779668787 1107 32-33 -0.4354437431133036 1107 34-35 -0.6415671075969556 1107 36-37 -0.23188878702661242 1107 38-39 -0.561740511538936 1107 40-41 -0.052285425851088974 1107 42-43 -0.39991953972592853 1107 44-45 -0.8081482819967647 1107 46-47 -1.7932493094213626 1107 48-49 -2.086846871405406 1107 50-51 -2.006600975525849 1107 52-53 -1.551992708840288 1107 54-55 -1.3102949020816261 1107 56-57 -1.0021321848270333 1107 58-59 -1.2756949491503384 1107 60-61 -1.0529650739398164 1107 62-63 -1.1281370106577988 1107 64-65 -1.3250693834879534 1107 66-67 -0.9362164443080481 1107 68-69 -1.0570600994051276 1107 70-71 -1.1685988637809892 1107 72-73 -1.3229900150744136 1107 74-75 -1.2436213234178979 1107 76-77 -1.4117876498184536 1107 78-79 -1.5494268736089687 1107 80-81 -1.2375006141872191 1107 82-83 -1.1323925008382751 1107 84-85 -1.1655031166669012 1107 86-87 -1.0856635746852987 1107 88-89 -1.03606029944471 1107 90-91 -1.0658733559903304 1107 92-93 -0.8588713941039892 1107 94-95 -0.840354568756851 1107 96-97 -1.0989436151062648 1107 98-99 -0.509290034711853 1107 100-101 -0.18009951056101414 1108 1 -0.05667371877614613 1108 2 0.23130816174644764 1108 3 0.14577582945903345 1108 4 0.09897617550673488 1108 5 0.14409898273060406 1108 6 0.07447555132878847 1108 7 -0.0037979115581663336 1108 8 0.1088484636234881 1108 9 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2316 88-89 -0.601278310274008 2316 90-91 -0.42457954008836296 2316 92-93 -0.6320831353408103 2316 94-95 -0.7065415166052418 2316 96-97 -1.0976896966109635 2316 98-99 -2.08945532396805 2316 100-101 -2.901729604604901 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 9.0 5 24.0 6 55.0 7 117.0 8 274.0 9 437.0 10 812.0 11 1473.0 12 2206.0 13 3291.0 14 4026.0 15 5763.0 16 7779.0 17 10785.0 18 8069.0 19 4075.0 20 3162.0 21 3265.0 22 3615.0 23 3973.0 24 4358.0 25 4714.0 26 5380.0 27 6056.0 28 6903.0 29 7953.0 30 9482.0 31 10766.0 32 12774.0 33 15921.0 34 20834.0 35 27930.0 36 32078.0 37 33489.0 38 17019.0 39 908.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.99194188432635 18.71956756447565 15.098778055041148 13.189712496156844 2 15.49419633441411 26.631443105488366 36.81243453672557 21.061926023371953 3 24.838608205780794 32.53251070583441 30.284106750931176 12.34477433745362 4 11.02453391284456 18.468346105455794 37.70480670250486 32.802313279194784 5 9.726422040647094 44.30969282349324 29.812991914902742 16.150893220956917 6 23.133864234244538 38.58372412215487 17.054000343131648 21.228411300468945 7 22.49835582751916 41.718374699759806 18.96409985130962 16.819169621411415 8 27.41693354683747 27.46911815166419 18.887610088070456 26.226338213427887 9 28.58429600823516 19.524190781196385 22.016899233672653 29.8746139768958 10-11 24.88669507034199 28.52603511380533 22.428478497083383 24.158791318769303 12-13 33.13996297011159 27.737209318950296 23.866049025284696 15.25677868565342 14-15 26.844916727876456 26.600077562188257 32.328777895133776 14.22622781480151 16-17 16.68173824774105 30.629324888482213 32.30655953334096 20.382377330435776 18-19 15.95260434817575 28.32551448051542 39.43882976346853 16.283051407840297 20-21 19.984737790232185 23.690023447329292 38.346927542033626 17.978311220404898 22-23 18.031031968431886 24.679028937435664 38.62232643257463 18.66761266155782 24-25 19.489520187578634 25.199087841701935 39.28035285371154 16.031039117007893 26-27 19.393251599528185 22.66915680737749 42.76137541551989 15.176216177574437 28-29 16.336746912587348 26.630386217003554 41.74557217664826 15.287294693760836 30-31 16.80252206719436 26.656134224301702 40.08839387998378 16.45294982852016 32-33 16.460678073340876 26.960466275958584 39.86688043010061 16.711975220599932 34-35 18.372690277854726 24.839822925025512 39.905850730157255 16.881636066962507 36-37 18.504790482300862 24.931775168127075 41.08519927727251 15.478235072299556 38-39 18.766352601404 25.847118367813792 40.59001615601275 14.796512874769455 40-41 16.674083327525103 27.52385394718265 39.92218761113821 15.879875114154027 42-43 15.450853952344525 29.696916620793683 36.7991263878066 18.05310303905518 44-45 17.47360783213384 27.78169964310033 35.41742249450889 19.327270030256944 46-47 18.65492393915132 29.409420393457626 33.69517042205193 18.240485245339126 48-49 18.866366661543466 28.89842836871321 32.91114582179124 19.324059147952088 50-51 17.62409927942354 30.64773247169164 33.74556788287773 17.98260036600709 52-53 17.675958044932454 32.26479802555978 30.685496049511123 19.37374787999664 54-55 15.737636361036845 30.145082424259755 31.907946470697567 22.209334744005833 56-57 19.097157054633527 30.496286564603054 29.00662251655629 21.399933864207128 58-59 20.85052449427597 29.01361468440961 29.36578023439567 20.77008058691875 60-61 19.128474723514703 29.468586944281082 32.35007588040034 19.052862451803882 62-63 20.810201156102426 29.315811863455508 31.24854772229233 18.625439258149736 64-65 19.82360743179714 31.994301383183714 30.86905693969262 17.313034245326524 66-67 19.66042928886744 29.996783014315586 30.208567905206156 20.134219791610818 68-69 19.068631130460272 31.247341575788766 30.32426520261214 19.359762091138823 70-71 20.572886880933318 31.310762896031108 28.615928457051353 19.500421765984218 72-73 19.333681230698847 28.811978153951735 31.150456079149038 20.70388453620039 74-75 17.86505106827869 33.031123502131194 29.89125092709255 19.212574502497564 76-77 18.494378448126415 29.565377459819885 30.96687832749226 20.973365764561436 78-79 17.783632295010474 29.287282034416933 32.7184302903348 20.21065538023779 80-81 18.238188782851637 30.988994489200945 30.906591687863195 19.866225040084228 82-83 17.165619782710507 29.712675935492406 31.491782824906423 21.629921456890667 84-85 17.122436684704397 29.48820396758571 30.515585922346585 22.873773425363304 86-87 17.413304354353816 29.96732715385193 30.040608701558213 22.578759790236038 88-89 17.80932867042846 28.543307086614174 29.786986776516695 23.860377466440674 90-91 17.386873216469628 27.04976362635084 31.26980210411884 24.2935610530607 92-93 17.44591615820138 29.939770774970814 30.93876427293189 21.675548793895913 94-95 16.68838749237983 29.354757397147868 30.255054337818727 23.70180077265357 96-97 14.515347493281505 28.766782828544713 31.472776459607672 25.24509321856611 98-99 17.138667858739385 28.821099687080913 28.835940992400538 25.204291461779167 100-101 17.10827731610882 27.74663226055654 29.7546686981293 25.390421725205336 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1759.0 1 1283.0 2 1387.5 3 1748.5 4 1303.0 5 1150.0 6 1630.5 7 2847.0 8 3928.5 9 4184.5 10 3499.0 11 2565.0 12 2259.5 13 2201.5 14 2024.5 15 1817.0 16 1713.5 17 1642.0 18 1604.5 19 1588.0 20 1603.5 21 1642.5 22 1653.0 23 1672.5 24 1756.0 25 1925.0 26 2149.5 27 2336.5 28 2593.5 29 2964.5 30 3350.5 31 3602.5 32 3946.0 33 4443.5 34 4939.5 35 5305.5 36 5962.0 37 6461.5 38 6481.0 39 6463.0 40 6056.5 41 5624.0 42 5410.0 43 5253.0 44 5349.5 45 5428.0 46 6217.0 47 12432.5 48 30790.0 49 29502.5 50 12079.5 51 6766.5 52 3714.0 53 2637.5 54 2340.0 55 2242.5 56 2473.0 57 2714.5 58 2788.5 59 3074.0 60 3255.0 61 3212.0 62 2741.0 63 1576.0 64 747.0 65 589.0 66 466.0 67 337.5 68 253.5 69 190.5 70 161.5 71 139.0 72 122.0 73 132.5 74 108.5 75 85.0 76 69.0 77 45.0 78 38.5 79 29.5 80 17.5 81 19.0 82 16.5 83 10.0 84 7.0 85 3.0 86 1.0 87 0.0 88 0.5 89 0.5 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020730870410614204 2 0.013939723207137138 3 0.007863433604026079 4 0.0 5 7.148576003660071E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.148576003660071E-4 14-15 1.7871440009150177E-4 16-17 0.0 18-19 0.0012510008006405124 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0021445728010980212 28-29 0.003931716802013039 30-31 0.0019658584010065197 32-33 0.009829292005032597 34-35 0.0026807160013725263 36-37 8.935720004575088E-4 38-39 0.0014297152007320141 40-41 1.7871440009150177E-4 42-43 0.006433718403294064 44-45 0.0019658584010065197 46-47 0.0 48-49 0.0010722864005490106 50-51 0.0 52-53 1.7871440009150177E-4 54-55 0.0014297152007320141 56-57 0.01733529680887567 58-59 0.027879446414274277 60-61 0.021267013610888712 62-63 0.015369438407869153 64-65 0.02108829921079721 66-67 0.003931716802013039 68-69 0.0017871440009150177 70-71 0.0 72-73 0.0 74-75 0.0012510008006405124 76-77 0.001608429600823516 78-79 0.010722864005490105 80-81 0.018765012009607687 82-83 0.0203734416104312 84-85 0.0302027336154638 86-87 0.011795150406039118 88-89 0.043606313622326434 90-91 0.04789545922452247 92-93 0.03377702161729384 94-95 0.03234730641656182 96-97 0.04914646002516299 98-99 0.05397174882763354 100-101 0.06237132563193412 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 279776.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.455421480041174 #Duplication Level Percentage of deduplicated Percentage of total 1 78.97881698790431 27.21248427313279 2 10.853959625718376 7.479555072629532 3 3.8092076599099562 3.937435662815967 4 1.7998298719890455 2.4805558732700446 5 1.0373659204547812 1.7871440009150177 6 0.6234569181933236 1.2888882534599106 7 0.42220792962509596 1.018314651721377 8 0.3444054855909874 0.9493308932860575 9 0.28216353036370045 0.8749857028479926 >10 1.5145542438639807 9.776392542605512 >50 0.15145542438639806 3.7726609859316023 >100 0.12863337413639286 8.382062793091617 >500 0.02489678209091475 5.829306302184604 >1k 0.025934148011369527 17.78958881390827 >5k 0.003112097761364344 7.421294178199703 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7389 2.641041404552213 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6858 2.4512467116550383 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6516 2.3290060619924513 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4437 1.5859115864119866 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3735 1.3349965686835183 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3695 1.320699416676198 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3616 1.2924625414617408 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3261 1.1655753173967747 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2644 0.9450417476838613 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2176 0.7777650691982158 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2047 0.7316567539746083 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1914 0.6841187235502688 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1806 0.6455164131305045 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1795 0.6415846963284914 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1733 0.6194241107171451 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1601 0.5722435090929887 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1565 0.5593760722864005 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1503 0.5372154866750544 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1397 0.49932803385565594 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1389 0.4964686034541919 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1331 0.4757377330435777 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1319 0.47144858744138163 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1307 0.4671594418391856 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1183 0.4228382706164932 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1137 0.40639654580807505 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1091 0.3899548209996569 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1085 0.38781024819855886 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1004 0.3588585153837356 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 999 0.3570713713828205 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 980 0.35028022417934346 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 955 0.3413445041747684 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 917 0.3277622097678142 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 792 0.2830836097449388 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 756 0.2702161729383507 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 720 0.25734873613176257 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 719 0.25699130733157954 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 695 0.24841301612718744 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 691 0.24698330092645546 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 690 0.24662587212627243 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 626 0.22375042891456023 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 611 0.21838899691181518 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 602 0.2151721377101681 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 601 0.2148147089099851 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 600 0.2144572801098021 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 589 0.21052556330778907 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 572 0.20444927370467803 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 560 0.20016012810248196 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 547 0.19551355370010293 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 537 0.1919392656982729 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 535 0.1912244080979069 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 508 0.1815738304929658 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 507 0.1812164016927828 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 475 0.1697786800869267 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 394 0.1408269472721034 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 386 0.13796751687063935 No Hit CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT 350 0.12510008006405124 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 339 0.1211683632620382 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 334 0.11938121926112319 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 326 0.11652178885965915 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 314 0.11223264325746311 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 309 0.11044549925654809 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 307 0.10973064165618207 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 306 0.10937321285599907 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 304 0.10865835525563307 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 294 0.10508406725380304 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 293 0.10472663845362004 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 291 0.10401178085325403 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 291 0.10401178085325403 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 290 0.10365435205307104 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 288 0.10293949445270502 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 287 0.10258206565252202 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 286 0.10222463685233901 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 286 0.10222463685233901 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 284 0.10150977925197302 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.5742880018300354E-4 0.0 20-21 0.0 0.0 0.0 3.5742880018300354E-4 0.0 22-23 0.0 0.0 0.0 3.5742880018300354E-4 0.0 24-25 0.0 0.0 0.0 7.148576003660071E-4 0.0 26-27 0.0 0.0 0.0 0.0026807160013725267 0.0 28-29 0.0 0.0 0.0 0.006612432803385566 0.0 30-31 0.0 0.0 0.0 0.01519072400777765 0.0 32-33 0.0 0.0 0.0 0.020909584810705706 0.0 34-35 0.0 0.0 0.0 0.025913588013267756 0.0 36-37 0.0 0.0 0.0 0.03073887681573831 0.0 38-39 0.0 0.0 0.0 0.03842359601967288 0.0 40-41 0.0 0.0 0.0 0.05343560562735903 0.0 42-43 0.0 0.0 0.0 0.07291547523733272 0.0 44-45 0.0 0.0 0.0 0.10526278165389455 0.0 46-47 0.0 0.0 0.0 0.18085897289259978 0.0 48-49 0.0 0.0 0.0 0.23107771931831178 0.0 50-51 0.0 0.0 0.0 0.2941639025506119 0.0 52-53 0.0 0.0 0.0 0.35671394258263756 0.0 54-55 0.0 0.0 0.0 0.4205149834153037 0.0 56-57 0.0 0.0 0.0 0.4957537458538259 0.0 58-59 0.0 0.0 0.0 0.607628960311106 0.0 60-61 0.0 0.0 0.0 0.7641827747912615 0.0 62-63 0.0 0.0 0.0 0.9425397460825804 0.0 64-65 0.0 0.0 0.0 1.0490535285371154 0.0 66-67 0.0 0.0 0.0 1.2215129246254146 0.0 68-69 0.0 0.0 0.0 1.4209581951275307 0.0 70-71 0.0 0.0 0.0 1.56964857600366 0.0 72-73 0.0 0.0 0.0 1.8173467345304815 0.0 74-75 0.0 0.0 0.0 2.028765869838728 0.0 76-77 0.0 0.0 0.0 2.1906811163216284 0.0 78-79 0.0 0.0 0.0 2.4181845476381105 0.0 80-81 0.0 0.0 0.0 2.6174511037401347 0.0 82-83 0.0 0.0 0.0 2.8687235502687862 0.0 84-85 0.0 0.0 0.0 3.2658269472721035 0.0 86-87 0.0 0.0 0.0 3.847721033970033 0.0 88-89 0.0 0.0 0.0 4.446950417476838 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTCAA 35 3.4161712E-6 67.83736 9 GATAGTC 45 2.0461448E-7 63.314865 7 TATATGG 25 0.0050160065 57.003754 2 AGATAGT 50 4.2388456E-7 56.98338 6 CGTCACT 60 2.3348548E-8 55.44014 1 ATGGGCG 35 2.788303E-4 54.269886 5 ATGGGCA 135 0.0 42.20991 5 AGGCTTA 80 2.2595668E-7 41.55038 6 TCGACCG 35 4.314861E-6 40.70241 18-19 TGGGATA 165 0.0 40.291283 6 GCTTATG 60 8.254026E-5 39.6001 1 GATATAG 85 3.6155143E-7 39.134216 1 ATATAGG 135 7.2759576E-12 38.706257 2 CGATCCG 25 0.0016174231 37.98892 70-71 CATGGGT 155 1.8189894E-12 36.76347 4 TACCTGG 130 2.0372681E-10 36.54087 2 GACCGTC 40 1.0787742E-5 35.614613 20-21 GTTCGAC 40 1.0787742E-5 35.614613 16-17 ATAGGCT 95 8.6884756E-7 34.989796 4 CGACCGT 35 2.0555088E-4 33.91868 18-19 >>END_MODULE