FastQCFastQC Report
Thu 26 May 2016
SRR936695_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936695_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124415
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73300.34503616289660916No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58840.2769703659595701No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32710.15397179929533542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52200.048.3205681
GTATCAA94000.040.477411
ATCAACG115050.032.741973
TCAACGC115500.032.6151734
CAACGCA117900.031.8311255
TATCAAC119350.031.6021292
AACGCAG121250.030.9146756
ACGCAGA136600.027.473577
CGCAGAG139600.026.8856968
GTACATG106150.026.7601911
TACATGG109700.025.245282
GCAGAGT147550.024.92259
ACATGGG110500.024.1167553
GAGTACT74200.023.14506712-13
CCTATAC5300.021.5106563
GTACTTT79450.021.49758714-15
ATTCGCG3650.021.4806580-81
GTATAGG7300.021.4733161
GTATAGC7250.020.9662131
CAGAGTA145950.020.91381810-11
Produced by FastQC (version 0.11.5)