FastQCFastQC Report
Thu 26 May 2016
SRR936695_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936695_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2124415
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91090.42877686327765524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79680.3750679598854273No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44770.21074036852498215No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.10732366322022768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60700.051.0404741
GTATCAA104450.039.6246531
ATCAACG135150.030.4378853
TCAACGC137650.029.9167674
TATCAAC138450.029.7845152
CAACGCA140750.029.3253445
AACGCAG144100.028.6086166
ACGCAGA163500.025.2140757
CGCAGAG165850.024.8568068
TAAGGTG10200.024.6801325
GTACATG104000.024.2157731
GCAGAGT174500.023.1886279
TACATGG107950.023.0166872
TATAACG1452.3326344E-522.934742
GTGGTAT30550.022.7089731
GTAAGGT9000.022.6933024
GAGTACT103250.022.51956412-13
TGGTATC30650.021.855082
ACATGGG110050.021.841033
GTACTTT106950.021.47425714-15
Produced by FastQC (version 0.11.5)