FastQCFastQC Report
Thu 26 May 2016
SRR936694_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936694_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093413
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73070.34904722575048497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57280.27362015999709566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32680.15610870860169493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48800.048.110191
GTATCAA88450.041.9646071
ATCAACG110350.033.5071263
TCAACGC111100.033.3664864
TATCAAC112500.032.8667682
CAACGCA113200.032.7062955
AACGCAG116750.031.7118026
ACGCAGA132400.027.995277
CGCAGAG135050.027.3755638
GTACATG108050.026.610961
TACATGG111200.025.6007082
GCAGAGT143700.025.2316599
ACATGGG111150.024.71433
GAGTACT71950.023.80706212-13
TGGTATC26000.022.8489692
GTGGTAT26700.022.783931
TAAGGTG8500.021.8065015
GTACTTT78100.021.71761514-15
GTATAGG6250.021.2915841
ATGGGCG2709.949872E-1021.1231065
Produced by FastQC (version 0.11.5)