##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936694_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2093413 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.43684643211827 31.0 31.0 34.0 30.0 34.0 2 31.490825269547862 31.0 31.0 34.0 28.0 34.0 3 31.530717063474814 33.0 31.0 34.0 28.0 34.0 4 35.176477837865725 37.0 35.0 37.0 33.0 37.0 5 35.10488852414693 37.0 35.0 37.0 32.0 37.0 6 35.2164556157815 37.0 35.0 37.0 32.0 37.0 7 35.06556326916858 37.0 35.0 37.0 32.0 37.0 8 35.04666446611347 37.0 35.0 37.0 32.0 37.0 9 36.570342307036405 39.0 37.0 39.0 32.0 39.0 10-11 36.44042264952019 39.0 37.0 39.0 32.0 39.0 12-13 36.353369593099885 39.0 37.0 39.0 32.0 39.0 14-15 37.44917175922764 40.0 37.0 41.0 32.0 41.0 16-17 37.38578388497635 40.0 37.0 41.0 32.0 41.0 18-19 37.34308447496982 40.0 37.0 41.0 32.0 41.0 20-21 37.25668441917577 39.5 37.0 41.0 31.0 41.0 22-23 37.153648133454794 39.0 36.0 41.0 31.0 41.0 24-25 37.01443480096856 39.0 36.0 41.0 31.0 41.0 26-27 36.822246971811104 39.0 36.0 41.0 30.5 41.0 28-29 36.924758277511415 39.0 36.0 41.0 30.0 41.0 30-31 36.84664588401811 39.0 36.0 41.0 30.0 41.0 32-33 36.699010658670794 39.0 36.0 41.0 30.0 41.0 34-35 36.5365484498281 39.0 35.0 41.0 30.0 41.0 36-37 36.30210832740601 39.0 35.0 40.5 30.0 41.0 38-39 36.123917258562926 39.0 35.0 40.0 29.5 41.0 40-41 35.96242881839369 38.5 35.0 40.0 29.0 41.0 42-43 35.691469624006345 38.0 35.0 40.0 28.0 41.0 44-45 35.42724608092144 38.0 34.5 40.0 27.0 41.0 46-47 35.07078703533416 38.0 34.0 40.0 26.0 41.0 48-49 34.82037132663263 38.0 34.0 40.0 24.5 41.0 50-51 34.124250446519625 37.5 33.5 39.5 24.0 40.5 52-53 34.30235385946299 38.0 33.5 39.5 24.0 40.5 54-55 34.646436465236434 38.0 34.0 40.0 24.0 41.0 56-57 34.580673283293834 38.0 34.0 40.0 24.5 41.0 58-59 34.3470734155181 38.0 34.0 40.0 24.0 41.0 60-61 34.04397292841881 37.5 33.5 40.0 23.0 41.0 62-63 33.723888453926676 37.0 33.0 40.0 22.5 41.0 64-65 33.41294813780176 36.5 33.0 39.5 22.0 41.0 66-67 33.06345570606469 36.0 32.5 39.0 21.5 41.0 68-69 32.69341572828677 35.5 32.0 39.0 20.5 40.5 70-71 32.309884862662074 35.0 32.0 38.5 19.5 40.0 72-73 31.912315677795064 35.0 31.0 37.5 18.5 40.0 74-75 31.483673312432856 35.0 31.0 37.0 17.5 39.0 76-77 31.068852634429994 35.0 31.0 36.5 14.0 39.0 78-79 30.631362038928774 35.0 30.5 36.0 9.5 38.5 80-81 30.233636410970984 34.0 30.0 35.5 7.5 37.0 82-83 29.83866967483244 34.0 30.0 35.0 6.5 37.0 84-85 29.46231202347554 34.0 29.0 35.0 2.0 36.5 86-87 29.122749787070205 34.0 29.0 35.0 2.0 36.0 88-89 28.75797608976346 34.0 29.0 35.0 2.0 36.0 90-91 28.425510876258052 34.0 29.0 35.0 2.0 35.0 92-93 28.08191073619969 34.0 28.5 35.0 2.0 35.0 94-95 27.6797325706872 34.0 27.0 35.0 2.0 35.0 96-97 27.108887496160577 33.0 26.0 35.0 2.0 35.0 98-99 26.46961469141541 33.0 25.0 35.0 2.0 35.0 100-101 24.374610265628426 31.0 13.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2634194256670952 1101 2 0.2873803501727359 1101 3 0.2823505515501701 1101 4 0.3563710495123473 1101 5 0.3040381625037867 1101 6 0.21011288940426454 1101 7 0.3005874417872221 1101 8 0.25744150772468544 1101 9 0.4536619926212282 1101 10-11 0.3739481311314137 1101 12-13 0.5707969269222701 1101 14-15 0.5013087410899004 1101 16-17 0.5627095008861858 1101 18-19 0.7068511748023809 1101 20-21 0.5851406814021161 1101 22-23 0.5706047845706621 1101 24-25 0.5911027881589916 1101 26-27 0.5421858337668866 1101 28-29 0.617615588589409 1101 30-31 0.49077323345176893 1101 32-33 0.43757812395951845 1101 34-35 0.4538009254773172 1101 36-37 0.4807804099666768 1101 38-39 0.3871921867668462 1101 40-41 0.3231591997107799 1101 42-43 0.2075794819163974 1101 44-45 0.3536037499328444 1101 46-47 0.4325336917766549 1101 48-49 0.37188572927257013 1101 50-51 0.47119489683954896 1101 52-53 0.4059995055653758 1101 54-55 0.4293380527017945 1101 56-57 0.35447666726241067 1101 58-59 0.39608153742700125 1101 60-61 0.33298104077528734 1101 62-63 0.43319010482680653 1101 64-65 0.4715841342703655 1101 66-67 0.37999227244960565 1101 68-69 0.32488867326652837 1101 70-71 0.3486661807335736 1101 72-73 0.3127162153827534 1101 74-75 0.3226452242533533 1101 76-77 0.21008252695977347 1101 78-79 0.31618751039673754 1101 80-81 0.1774281181918944 1101 82-83 0.09747282971228444 1101 84-85 0.1428908466718788 1101 86-87 0.08574215147178421 1101 88-89 0.11969781145068126 1101 90-91 0.23934090282552134 1101 92-93 0.151968078562696 1101 94-95 -0.02501340221838788 1101 96-97 -0.06768118011424917 1101 98-99 -0.1952947514665695 1101 100-101 0.3774051289977223 1102 1 0.15211506067837988 1102 2 0.2561054160005689 1102 3 0.25301275786485533 1102 4 0.2897505006159733 1102 5 0.18724803696984083 1102 6 0.2369905114356996 1102 7 0.16327906079921917 1102 8 0.24950835605826427 1102 9 0.41886432301384957 1102 10-11 0.4524595905776465 1102 12-13 0.42442294200395025 1102 14-15 0.3784503700998343 1102 16-17 0.34547021242858733 1102 18-19 0.4610542713798509 1102 20-21 0.13320567639667757 1102 22-23 -0.11158284141465202 1102 24-25 -0.017372953742842867 1102 26-27 0.06720680031165926 1102 28-29 0.12743556750638874 1102 30-31 0.2108179297248327 1102 32-33 0.35595557547599554 1102 34-35 0.1940192881584224 1102 36-37 -0.017421902953820734 1102 38-39 0.07078243577044674 1102 40-41 -0.3119447011666381 1102 42-43 0.09597817176619117 1102 44-45 0.08117173104224662 1102 46-47 0.26047043169896256 1102 48-49 0.2062505918025792 1102 50-51 0.3094416439513026 1102 52-53 0.03715583962973312 1102 54-55 0.1504627039873867 1102 56-57 0.23062545919469102 1102 58-59 0.19686304273431432 1102 60-61 -0.18102990130203978 1102 62-63 -0.01852099818816555 1102 64-65 0.09436978564719567 1102 66-67 -0.03678081776671149 1102 68-69 0.08171744130730474 1102 70-71 0.08855516158477883 1102 72-73 -0.13106553776983176 1102 74-75 -0.1494134574816144 1102 76-77 0.03961958370987162 1102 78-79 -0.07933398063521224 1102 80-81 0.1062010770437567 1102 82-83 -0.006990391150203834 1102 84-85 -0.13017429392368385 1102 86-87 -0.2950602103706572 1102 88-89 -0.4616854660473635 1102 90-91 -0.580439026108241 1102 92-93 -0.5396904883739673 1102 94-95 -0.4053817921754117 1102 96-97 -0.5176873762459202 1102 98-99 -0.563520988250044 1102 100-101 -0.23386048994567687 1103 1 0.1975052862912321 1103 2 0.2820273497429078 1103 3 0.3020250305886698 1103 4 0.19179544734286225 1103 5 0.31820477175067197 1103 6 0.2266304286875993 1103 7 0.29770414831962455 1103 8 0.06157852117404161 1103 9 0.3591972598529054 1103 10-11 0.42490425257261677 1103 12-13 0.4293188432337516 1103 14-15 0.5204146822329818 1103 16-17 0.561762238497451 1103 18-19 0.45496838006663154 1103 20-21 0.43632090405963453 1103 22-23 0.2647312327796598 1103 24-25 0.3653712601036787 1103 26-27 0.3161409577444019 1103 28-29 0.36197089565401086 1103 30-31 0.18823052133488005 1103 32-33 0.25967940917835364 1103 34-35 0.2387733475750693 1103 36-37 0.41508200492423697 1103 38-39 0.1923999971699928 1103 40-41 0.2268430070034242 1103 42-43 0.40709658152823636 1103 44-45 0.34217089662891453 1103 46-47 0.2795855139525045 1103 48-49 0.31698823179084457 1103 50-51 -0.2150139372763391 1103 52-53 0.050347348057989905 1103 54-55 0.39128276237094894 1103 56-57 0.39850722443119224 1103 58-59 0.42893131414606245 1103 60-61 0.46506057209860074 1103 62-63 0.25623102380584584 1103 64-65 0.39973885926240627 1103 66-67 0.2852547957046241 1103 68-69 0.36594390125588916 1103 70-71 0.5407501251774747 1103 72-73 0.3546014053438107 1103 74-75 0.15411897684954923 1103 76-77 0.09601738667504023 1103 78-79 0.2584047507690812 1103 80-81 0.5000400293520002 1103 82-83 0.39951828649487453 1103 84-85 0.28906734524622735 1103 86-87 0.3207883169785717 1103 88-89 0.41377906208639814 1103 90-91 0.48209108082685503 1103 92-93 0.3191204345784868 1103 94-95 0.3254687599153989 1103 96-97 0.29753563699225083 1103 98-99 0.22193544937142917 1103 100-101 0.30447010850706135 1104 1 0.24071300864507705 1104 2 0.28874865771925684 1104 3 0.38671906059870054 1104 4 0.35220077487056045 1104 5 0.32702368703530027 1104 6 0.3773486630902809 1104 7 0.3982703048584426 1104 8 0.3779458345718325 1104 9 0.6164511071560597 1104 10-11 0.5164556602266686 1104 12-13 0.510026031981063 1104 14-15 0.44146332580515235 1104 16-17 0.24140808383693013 1104 18-19 0.41208028803016816 1104 20-21 0.406500302578344 1104 22-23 0.3884439070021912 1104 24-25 0.4183090309090005 1104 26-27 0.31023660138060905 1104 28-29 0.14339525159092403 1104 30-31 0.20322646592517657 1104 32-33 0.271108194049539 1104 34-35 0.19825553578876765 1104 36-37 0.26809368023533153 1104 38-39 0.2873843624983792 1104 40-41 0.27752682378428517 1104 42-43 0.18916553416532622 1104 44-45 -0.009130836525130803 1104 46-47 0.30884209332114665 1104 48-49 0.28148178582384276 1104 50-51 0.1868953069233612 1104 52-53 0.3082135088656557 1104 54-55 0.41171615760264046 1104 56-57 0.5403152968309186 1104 58-59 0.42849749202913756 1104 60-61 0.46218603366826017 1104 62-63 0.4023674456538444 1104 64-65 0.3531683484942221 1104 66-67 0.3778029803751721 1104 68-69 0.4073533695703304 1104 70-71 0.4131287830503112 1104 72-73 0.2392367386344496 1104 74-75 0.19569759843713186 1104 76-77 0.21941016377691724 1104 78-79 0.3438685082128927 1104 80-81 0.45853771349962 1104 82-83 0.19825310538881524 1104 84-85 0.3551854643898942 1104 86-87 0.29824984394704757 1104 88-89 0.2460334429960902 1104 90-91 0.14768199670912097 1104 92-93 0.08456327464593372 1104 94-95 0.11563465818865026 1104 96-97 0.35117750162694605 1104 98-99 0.37547806318970345 1104 100-101 0.5958819780509366 1105 1 0.22082369353279319 1105 2 0.2126600975141777 1105 3 0.1814125793392094 1105 4 0.3632984640430479 1105 5 0.42729709499870694 1105 6 0.43492570604252734 1105 7 0.4751357494114927 1105 8 0.20838755979298895 1105 9 0.4490989995018637 1105 10-11 0.40779174761885884 1105 12-13 0.2735946129645299 1105 14-15 0.330494886827843 1105 16-17 0.4285891847227816 1105 18-19 0.3082136939034541 1105 20-21 0.14888437698711243 1105 22-23 -0.019288589662814104 1105 24-25 -0.021671466296986353 1105 26-27 0.03434199331146459 1105 28-29 0.18731534392565408 1105 30-31 0.07673724548367034 1105 32-33 0.013807325414454397 1105 34-35 0.1242355934518784 1105 36-37 -0.028617739887735638 1105 38-39 -0.02560205470465604 1105 40-41 -0.13087420837304364 1105 42-43 -0.005907359256291045 1105 44-45 0.26076193652935586 1105 46-47 0.24701024459766074 1105 48-49 0.25434577650430157 1105 50-51 0.23348762014837376 1105 52-53 0.2998971578706886 1105 54-55 0.20719655030192996 1105 56-57 0.42184850229723736 1105 58-59 0.38263672568766793 1105 60-61 0.49448321383166416 1105 62-63 0.26579140781012 1105 64-65 0.3392835579072013 1105 66-67 0.0742763403884581 1105 68-69 0.2949418160475332 1105 70-71 0.4201126736502374 1105 72-73 0.19245923339375182 1105 74-75 0.29164550492208363 1105 76-77 0.4287762691811423 1105 78-79 0.37605003942043425 1105 80-81 0.40842521511332563 1105 82-83 0.2744277299331337 1105 84-85 0.1462950181462972 1105 86-87 0.303091731460956 1105 88-89 0.4560388834918214 1105 90-91 0.4137230063667694 1105 92-93 0.465354559402094 1105 94-95 0.3718447381670096 1105 96-97 0.3802480375004045 1105 98-99 -0.009730550621164014 1105 100-101 0.13916734588377722 1106 1 0.06763494930409664 1106 2 0.058964249734646756 1106 3 0.03198251205622782 1106 4 0.11038769376239088 1106 5 0.17950493322426553 1106 6 0.2650503226902927 1106 7 0.5046039407107159 1106 8 0.36561877850656543 1106 9 0.41180176915258926 1106 10-11 0.33890717447578567 1106 12-13 0.46879984943924313 1106 14-15 0.40654247705044355 1106 16-17 0.40768567096475294 1106 18-19 0.4266945527436974 1106 20-21 0.406424081985314 1106 22-23 0.39545649698975893 1106 24-25 0.24300018979290172 1106 26-27 0.31461308147817846 1106 28-29 0.07389772846476461 1106 30-31 0.07721728163915031 1106 32-33 0.1704651049529886 1106 34-35 0.07044837270742477 1106 36-37 0.22448250216039156 1106 38-39 0.26362864890818827 1106 40-41 0.1623814374345045 1106 42-43 0.03448529432452574 1106 44-45 0.07756949719107809 1106 46-47 0.2685976202004241 1106 48-49 0.18226827598961393 1106 50-51 0.43285856977922776 1106 52-53 0.30644418664989104 1106 54-55 0.5135120093116399 1106 56-57 0.4039867896200988 1106 58-59 0.4074009317640517 1106 60-61 0.34524887252492675 1106 62-63 0.35366396384987553 1106 64-65 0.2170330516238792 1106 66-67 0.2240802248057534 1106 68-69 0.13418999043373958 1106 70-71 0.2536389647034767 1106 72-73 0.4059164041619461 1106 74-75 0.4074986733247563 1106 76-77 0.13792562296758604 1106 78-79 0.39895797582682135 1106 80-81 0.31084879278321864 1106 82-83 0.29569788386459095 1106 84-85 0.38848381897046025 1106 86-87 0.31757678656771304 1106 88-89 0.3923096238835093 1106 90-91 0.39825040144165413 1106 92-93 0.20739807803225574 1106 94-95 0.079583203970639 1106 96-97 0.15128042897040217 1106 98-99 0.34531893349888776 1106 100-101 0.47349622622046184 1107 1 0.448117158613055 1107 2 0.5433590926036835 1107 3 0.5937260345744697 1107 4 0.4866671353280623 1107 5 0.6138838810860392 1107 6 0.4458332470733737 1107 7 0.6195931337627414 1107 8 0.581837272246915 1107 9 0.6802884437395349 1107 10-11 0.7425743171641841 1107 12-13 0.7696761223713153 1107 14-15 0.8669108861555017 1107 16-17 0.8671344657255275 1107 18-19 1.001564416070309 1107 20-21 1.0660903176049459 1107 22-23 0.9604331089600819 1107 24-25 0.9982601589686553 1107 26-27 0.8520789036619263 1107 28-29 0.8664838615326076 1107 30-31 0.8216946838623684 1107 32-33 0.7139294874727824 1107 34-35 0.8592459787275999 1107 36-37 0.9933654275613222 1107 38-39 0.9367468185229484 1107 40-41 0.7831876350434115 1107 42-43 0.9548972294747884 1107 44-45 0.6486084625645816 1107 46-47 0.9158565185048033 1107 48-49 0.9310050193996986 1107 50-51 0.9592968312276042 1107 52-53 0.9870182153678968 1107 54-55 0.97964345332867 1107 56-57 1.024096944505878 1107 58-59 1.0115822300038602 1107 60-61 0.9664684823480698 1107 62-63 1.0662125217359915 1107 64-65 0.8433866422740621 1107 66-67 0.8806869970020088 1107 68-69 0.9508500174138277 1107 70-71 0.8494958002785609 1107 72-73 0.9630226796244337 1107 74-75 0.7827099656681362 1107 76-77 0.8055516710853112 1107 78-79 0.9405753182620877 1107 80-81 1.1063982854330519 1107 82-83 1.0938454941573603 1107 84-85 1.178063156360512 1107 86-87 1.0338654645173477 1107 88-89 1.26219591126193 1107 90-91 1.2038578948901382 1107 92-93 1.1009079205095347 1107 94-95 1.3354407449384489 1107 96-97 1.3315633056566298 1107 98-99 1.6026270270282836 1107 100-101 1.7510011574600561 1108 1 0.16466050981074076 1108 2 0.02355728147291103 1108 3 -0.06760766703093068 1108 4 0.03650214827948872 1108 5 0.1557837611067896 1108 6 0.09910019504605572 1108 7 0.25196355031484075 1108 8 -0.11524167050979628 1108 9 0.09224927561228924 1108 10-11 0.06461851629335058 1108 12-13 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2316 82-83 -2.495208299791816 2316 84-85 -2.864823776419829 2316 86-87 -2.80046366207392 2316 88-89 -3.1393935112907805 2316 90-91 -3.036761677667844 2316 92-93 -3.241785666992577 2316 94-95 -3.1556419452362263 2316 96-97 -3.568997063044282 2316 98-99 -3.931200587652075 2316 100-101 -4.573827297411825 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4418.0 3 1797.0 4 1761.0 5 1993.0 6 2158.0 7 2618.0 8 3158.0 9 3975.0 10 5088.0 11 6446.0 12 8210.0 13 9725.0 14 11026.0 15 13463.0 16 16389.0 17 20235.0 18 17647.0 19 12481.0 20 12793.0 21 13373.0 22 15008.0 23 16889.0 24 19699.0 25 22542.0 26 26626.0 27 31642.0 28 38093.0 29 45478.0 30 56191.0 31 69978.0 32 87677.0 33 113389.0 34 151214.0 35 206362.0 36 289417.0 37 379829.0 38 306849.0 39 47757.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.457736809265505 18.464257287523086 14.282561064985968 22.795444838225443 2 15.767669346062455 19.741665862497598 40.18701946917198 24.30364532226797 3 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22.6715376586277 26.643840360762322 28.04186600870855 22.64275597190143 48-49 22.788190242258484 26.82209768823942 27.754024604418305 22.63568746508379 50-51 22.690690966119156 26.93475935215791 27.616145273159848 22.758404408563088 52-53 22.977035623242536 26.84405638784281 27.585794268591034 22.59311372032362 54-55 22.603239676748878 26.830516725049762 27.661956369601555 22.904287228599802 56-57 22.662714812788128 27.14588269070326 27.426961442673477 22.76444105383513 58-59 22.824127329287496 26.716631380222267 27.5696206643533 22.889620626136935 60-61 22.66468296572049 26.734534142768666 27.97436315723728 22.62641973427356 62-63 22.58342889230335 26.862504236560458 27.948042989713546 22.606023881422644 64-65 22.402412989404 26.83796254497006 27.86878080309095 22.890843662534984 66-67 22.3711134598045 27.113650884641338 27.549050869116076 22.966184786438088 68-69 22.513770947832466 26.82490587075553 27.879979868774413 22.7813433126376 70-71 22.38041731400213 26.85383977420175 27.78999195488621 22.975750956909913 72-73 22.495640328862873 26.912636274804626 27.6531056073456 22.9386177889869 74-75 22.426121970106276 27.203323478311546 27.44547134758705 22.92508320399513 76-77 22.507780609502973 27.055119915732956 27.472609627175643 22.96448984758843 78-79 22.55782615240157 27.060536771961512 27.4337636397086 22.94787343592831 80-81 22.467574538188607 27.435170577088652 27.321694600072433 22.775560284650307 82-83 22.510074827930154 27.369590930457417 27.27264041701858 22.847693824593854 84-85 22.466451894456664 27.28021897306334 27.15223467323516 23.101094459244838 86-87 22.602218318143656 27.13635837052625 27.231377963241414 23.030045348088677 88-89 22.544573812370196 27.18865808610577 27.082127600114177 23.184640501409856 90-91 22.458494156384262 27.08835868043164 27.346439964092205 23.106707199091893 92-93 22.494234051523872 26.80922981738874 27.491827342838338 23.20470878824905 94-95 22.301784343391155 26.77582797765976 27.54355281682235 23.378834862126737 96-97 22.415511623757567 26.692830839806902 27.469778278099316 23.42187925833622 98-99 22.099942735740854 26.86496456997934 27.31882622841631 23.7162664658635 100-101 22.02140471104357 26.83084591379813 27.585530434450163 23.56221894070814 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 177.0 1 155.5 2 157.0 3 223.5 4 245.5 5 245.0 6 382.0 7 767.0 8 1312.0 9 1651.5 10 1574.0 11 1482.0 12 1594.0 13 1707.0 14 1880.5 15 2115.5 16 2292.0 17 2442.0 18 2698.0 19 2952.5 20 3260.0 21 3806.0 22 4413.5 23 5313.5 24 6545.5 25 8126.0 26 10349.0 27 13054.5 28 15939.0 29 19208.5 30 23223.5 31 27952.5 32 33482.0 33 37353.5 34 40587.0 35 44104.0 36 48762.0 37 53990.0 38 56477.0 39 57834.0 40 60215.0 41 64536.5 42 68192.0 43 71051.5 44 73912.5 45 77142.0 46 77927.5 47 77155.5 48 79530.0 49 79866.5 50 76213.0 51 72516.0 52 70454.5 53 70614.5 54 70423.0 55 66210.0 56 60427.0 57 54787.0 58 50175.5 59 44867.0 60 38142.0 61 32111.5 62 27094.0 63 21803.0 64 16663.0 65 13087.0 66 9655.5 67 7481.0 68 5507.5 69 3444.5 70 2513.5 71 2036.5 72 1740.0 73 1628.5 74 1325.0 75 1049.0 76 782.5 77 449.0 78 274.5 79 193.0 80 142.0 81 104.0 82 73.5 83 44.0 84 26.5 85 15.5 86 9.0 87 8.5 88 12.0 89 11.5 90 6.5 91 3.0 92 2.0 93 3.5 94 4.0 95 1.5 96 1.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015047198044533017 2 0.007404176815563866 3 0.008789474413314525 4 0.034680208826447526 5 0.045858127373814915 6 0.05956779670327834 7 0.026941649832116263 8 0.024362130167339173 9 0.014951660279170905 10-11 0.0449266341615343 12-13 0.043135301060994655 14-15 0.021257152793070454 16-17 0.023502290279080143 18-19 0.030643738239898195 20-21 0.023932210223209658 22-23 0.014521740335041389 24-25 0.003176630698290304 26-27 0.003057208491587661 28-29 0.0012658753910480159 30-31 0.006544336927304837 32-33 0.0013375287150696016 34-35 0.002149599720647574 36-37 0.006209954748537437 38-39 0.007284754608861224 40-41 0.004108123910570919 42-43 8.598398882590296E-4 44-45 0.0015286042457938304 46-47 0.0032482840223118895 48-49 4.060355027889862E-4 50-51 0.0015524886871343593 52-53 8.837243295995582E-4 54-55 0.002675057430139203 56-57 0.0023645596927123315 58-59 0.003296052904992947 60-61 9.314932122806154E-4 62-63 0.0013614131564101302 64-65 0.003630435083760347 66-67 0.002412328575393389 68-69 0.007045910195455938 70-71 0.010365847541789413 72-73 0.0037498572904629906 74-75 0.003057208491587661 76-77 0.0032482840223118895 78-79 0.03429805776499907 80-81 0.021925917150605254 82-83 0.003678203966441405 84-85 0.008956665502698226 86-87 0.007666905670309682 88-89 0.005517305949662107 90-91 0.013088673854609673 92-93 0.014593393659062976 94-95 0.007165332402158581 96-97 0.004418621647997791 98-99 0.023144023658972213 100-101 0.04217992340737351 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2093413.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.805023093641196 #Duplication Level Percentage of deduplicated Percentage of total 1 77.75770573879963 43.392705644622694 2 12.819761079428291 14.308141261849167 3 4.111512641339886 6.883291736993103 4 1.7864305556247788 3.987671936473082 5 0.9098805326647922 2.538795206890664 6 0.5588209207005022 1.8711008630940815 7 0.37896624934312195 1.4803754207412456 8 0.2578298371724754 1.151056001411179 9 0.19091478079940644 0.9588603376285488 >10 1.0602070040498945 11.117887572451975 >50 0.10360383368987384 3.977741684430495 >100 0.06042084643271765 6.001927926333706 >500 0.003088158225390897 1.1325743442567384 >1k 6.862573834201932E-4 0.5738746878122357 >5k 1.715643458550483E-4 0.6239953750110521 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7307 0.34904722575048497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5728 0.27362015999709566 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3268 0.15610870860169493 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.77688826810572E-5 0.0 0.0 0.0 0.0 8 4.77688826810572E-5 0.0 0.0 0.0 0.0 9 4.77688826810572E-5 0.0 0.0 0.0 0.0 10-11 4.77688826810572E-5 0.0 0.0 0.0 0.0 12-13 4.77688826810572E-5 0.0 0.0 0.0 0.0 14-15 4.77688826810572E-5 0.0 0.0 0.0 0.0 16-17 4.77688826810572E-5 0.0 0.0 0.0 0.0 18-19 7.16533240215858E-5 0.0 0.0 0.0 0.0 20-21 9.55377653621144E-5 0.0 0.0 2.38844413405286E-5 0.0 22-23 9.55377653621144E-5 0.0 0.0 1.910755307242288E-4 2.38844413405286E-5 24-25 9.55377653621144E-5 0.0 0.0 3.343821787674004E-4 4.77688826810572E-5 26-27 9.55377653621144E-5 0.0 0.0 5.97111033513215E-4 7.16533240215858E-5 28-29 9.55377653621144E-5 0.0 0.0 0.001146453184345373 9.55377653621144E-5 30-31 9.55377653621144E-5 0.0 0.0 0.002579519664777089 9.55377653621144E-5 32-33 9.55377653621144E-5 0.0 0.0 0.004633581620062549 9.55377653621144E-5 34-35 9.55377653621144E-5 0.0 0.0 0.007595252346288096 9.55377653621144E-5 36-37 9.55377653621144E-5 0.0 0.0 0.010676345279216284 9.55377653621144E-5 38-39 1.910755307242288E-4 0.0 0.0 0.01385297597750659 9.55377653621144E-5 40-41 1.910755307242288E-4 0.0 0.0 0.016838531145072666 9.55377653621144E-5 42-43 1.910755307242288E-4 0.0 0.0 0.01996739296068191 9.55377653621144E-5 44-45 2.3884441340528602E-4 0.0 0.0 0.023812788016507016 1.1942220670264301E-4 46-47 2.6272885474581465E-4 0.0 0.0 0.029831667234320222 1.4330664804317161E-4 48-49 2.8661329608634323E-4 0.0 0.0 0.036519310809668235 1.4330664804317161E-4 50-51 2.8661329608634323E-4 0.0 0.0 0.042179923407373504 1.4330664804317161E-4 52-53 2.8661329608634323E-4 0.0 0.0 0.05240246430111975 1.6719108938370022E-4 54-55 2.8661329608634323E-4 0.0 0.0 0.07136671072549947 1.910755307242288E-4 56-57 2.8661329608634323E-4 0.0 0.0 0.11001173681447474 1.910755307242288E-4 58-59 2.8661329608634323E-4 0.0 0.0 0.15422183773579318 1.910755307242288E-4 60-61 2.8661329608634323E-4 0.0 0.0 0.19814532536102525 1.910755307242288E-4 62-63 2.8661329608634323E-4 0.0 0.0 0.2502134074833776 1.910755307242288E-4 64-65 2.8661329608634323E-4 0.0 0.0 0.31627777223127973 1.910755307242288E-4 66-67 3.821510614484576E-4 0.0 0.0 0.4234950294089126 1.910755307242288E-4 68-69 3.821510614484576E-4 0.0 0.0 0.5486733864746229 1.910755307242288E-4 70-71 3.821510614484576E-4 0.0 0.0 0.6540515416690352 1.910755307242288E-4 72-73 4.0603550278898624E-4 0.0 0.0 0.7659740337907522 1.910755307242288E-4 74-75 4.299199441295148E-4 0.0 0.0 0.8895760177279877 2.3884441340528602E-4 76-77 4.299199441295148E-4 0.0 0.0 1.0322855547376462 2.3884441340528602E-4 78-79 4.299199441295148E-4 0.0 0.0 1.1551948898760063 2.3884441340528602E-4 80-81 5.493421508321578E-4 0.0 0.0 1.2649916667184162 2.3884441340528602E-4 82-83 5.732265921726865E-4 0.0 0.0 1.3688412176670346 2.3884441340528602E-4 84-85 5.97111033513215E-4 0.0 0.0 1.5601555928046689 2.3884441340528602E-4 86-87 6.209954748537436E-4 0.0 0.0 1.8348983215447694 2.3884441340528602E-4 88-89 6.209954748537436E-4 0.0 0.0 2.172409362127779 2.3884441340528602E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4880 0.0 48.11019 1 GTATCAA 8845 0.0 41.964607 1 ATCAACG 11035 0.0 33.507126 3 TCAACGC 11110 0.0 33.366486 4 TATCAAC 11250 0.0 32.866768 2 CAACGCA 11320 0.0 32.706295 5 AACGCAG 11675 0.0 31.711802 6 ACGCAGA 13240 0.0 27.99527 7 CGCAGAG 13505 0.0 27.375563 8 GTACATG 10805 0.0 26.61096 1 TACATGG 11120 0.0 25.600708 2 GCAGAGT 14370 0.0 25.231659 9 ACATGGG 11115 0.0 24.7143 3 GAGTACT 7195 0.0 23.807062 12-13 TGGTATC 2600 0.0 22.848969 2 GTGGTAT 2670 0.0 22.78393 1 TAAGGTG 850 0.0 21.806501 5 GTACTTT 7810 0.0 21.717615 14-15 GTATAGG 625 0.0 21.291584 1 ATGGGCG 270 9.949872E-10 21.123106 5 >>END_MODULE