FastQCFastQC Report
Thu 26 May 2016
SRR936694_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936694_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093413
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91620.43765850312384613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81950.39146599357126377No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45750.2185426382658367No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23320.11139703441222538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58700.047.6514131
GTATCAA102950.040.451881
ATCAACG133600.031.1417333
TCAACGC135250.030.7259644
TATCAAC135750.030.6135252
CAACGCA136850.030.3999795
AACGCAG141550.029.4576856
ACGCAGA162400.025.6172267
CGCAGAG164550.025.253658
GTGGTAT32550.025.0578211
TGGTATC31900.024.1195642
GTACATG104450.024.0236281
GCAGAGT174200.023.5002849
TACATGG110850.022.5369512
GAGTACT100450.022.5266112-13
ACATGGG109400.021.8805543
GTAAGGT8600.021.5377794
AGTACTT103650.021.4417112-13
GTACTTT105900.021.23278214-15
AGAGTAC160650.020.57365210-11
Produced by FastQC (version 0.11.5)