##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936694_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2093413 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56867135152022 31.0 31.0 34.0 30.0 34.0 2 31.784587178927428 31.0 31.0 34.0 30.0 34.0 3 32.051844523751406 33.0 31.0 34.0 30.0 34.0 4 35.850776220459124 37.0 35.0 37.0 35.0 37.0 5 35.66859525569011 37.0 35.0 37.0 33.0 37.0 6 35.77173782717505 37.0 35.0 37.0 33.0 37.0 7 35.71213372612093 37.0 35.0 37.0 33.0 37.0 8 35.6836505744447 37.0 35.0 37.0 33.0 37.0 9 37.33051194389258 39.0 37.0 39.0 34.0 39.0 10-11 37.221789728066085 39.0 37.0 39.0 33.5 39.0 12-13 37.097423919694776 39.0 37.0 39.0 33.0 39.0 14-15 38.23947329074578 40.0 38.0 41.0 33.0 41.0 16-17 38.15195401003051 40.0 37.0 41.0 33.0 41.0 18-19 38.211626659431275 40.0 37.5 41.0 33.5 41.0 20-21 38.28249179688862 40.0 38.0 41.0 34.0 41.0 22-23 38.2006730157881 40.0 38.0 41.0 33.0 41.0 24-25 38.095153703545364 40.0 37.0 41.0 33.0 41.0 26-27 37.90424393084403 40.0 37.0 41.0 33.0 41.0 28-29 37.81467727581705 40.0 37.0 41.0 33.0 41.0 30-31 37.78525928710675 40.0 37.0 41.0 33.0 41.0 32-33 37.609016949832636 40.0 36.5 41.0 33.0 41.0 34-35 37.44290352644222 40.0 36.0 41.0 32.0 41.0 36-37 37.20854174498773 39.0 36.0 41.0 31.5 41.0 38-39 37.03896961564679 39.0 36.0 41.0 31.0 41.0 40-41 36.78736207332237 39.0 35.0 41.0 31.0 41.0 42-43 36.52289013204752 39.0 35.0 41.0 30.0 41.0 44-45 36.22620428935905 39.0 35.0 40.0 30.0 41.0 46-47 35.79275183635527 39.0 35.0 40.0 28.5 41.0 48-49 35.31986545416504 38.0 35.0 40.0 27.0 41.0 50-51 35.10893120468823 38.0 34.5 40.0 26.5 41.0 52-53 34.825922070800175 38.0 34.0 40.0 26.0 41.0 54-55 34.538454189402664 38.0 34.0 40.0 25.5 41.0 56-57 34.25627121834057 38.0 33.5 40.0 24.5 41.0 58-59 33.88016483130658 37.0 33.0 40.0 23.5 41.0 60-61 33.52227749612714 37.0 33.0 40.0 23.0 41.0 62-63 33.230208277105376 36.0 32.0 39.0 22.0 40.5 64-65 32.80017727032363 36.0 32.0 39.0 21.5 40.0 66-67 32.411736957781386 35.0 31.0 39.0 20.0 40.0 68-69 31.970981359148915 35.0 31.0 38.0 19.0 40.0 70-71 31.513553226238685 35.0 31.0 37.5 18.0 39.5 72-73 31.109974477085984 34.5 30.0 37.0 17.0 39.0 74-75 30.744614894433155 34.0 30.0 36.5 13.5 39.0 76-77 29.33622462457241 32.5 28.5 35.0 11.5 37.0 78-79 30.134775842129578 34.0 30.0 35.0 10.5 37.5 80-81 29.989221190467433 34.0 30.0 35.0 7.5 37.0 82-83 29.661581828334874 34.0 29.5 35.0 7.0 37.0 84-85 29.22206033878647 34.0 29.0 35.0 2.0 36.0 86-87 28.82627627706525 34.0 29.0 35.0 2.0 36.0 88-89 28.423028805113944 33.5 29.0 35.0 2.0 35.0 90-91 27.991936373759025 33.0 27.5 35.0 2.0 35.0 92-93 27.5514275014056 33.0 27.0 35.0 2.0 35.0 94-95 27.033725547706066 33.0 25.5 34.5 2.0 35.0 96-97 26.37239283409437 32.5 25.0 34.0 2.0 35.0 98-99 25.526941410987703 32.0 23.5 34.0 2.0 35.0 100-101 22.75580021715734 29.0 8.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.38281954727745315 1101 2 0.4038667876471784 1101 3 0.17145692451165218 1101 4 0.12114211737877412 1101 5 0.2280820237658574 1101 6 0.16570154070935672 1101 7 0.19460390617113887 1101 8 0.07105406627908906 1101 9 0.19835084391835522 1101 10-11 0.2032563995984802 1101 12-13 0.17499466734803093 1101 14-15 0.3982225708606748 1101 16-17 0.3190647674666067 1101 18-19 0.31747303335283306 1101 20-21 0.09221311574889057 1101 22-23 0.15760452536071767 1101 24-25 0.037422705313453264 1101 26-27 0.16326878088377583 1101 28-29 0.2201506907102555 1101 30-31 0.04656823884749173 1101 32-33 0.25712468672799815 1101 34-35 0.29821633077944654 1101 36-37 0.2718530133265702 1101 38-39 0.4289941804886155 1101 40-41 0.4331524733919778 1101 42-43 0.4578418177151775 1101 44-45 0.3841449659176419 1101 46-47 0.4485906117720049 1101 48-49 0.46812402665658936 1101 50-51 0.4448715343146432 1101 52-53 0.378061207902725 1101 54-55 0.4319500453121421 1101 56-57 0.37785999619737964 1101 58-59 0.4648782559011053 1101 60-61 0.5662403905715934 1101 62-63 0.4368556438481619 1101 64-65 0.4398255610838362 1101 66-67 0.2415795955814275 1101 68-69 0.038788631757391556 1101 70-71 0.14208409848199466 1101 72-73 0.17799250299466962 1101 74-75 0.15099947009540315 1101 76-77 0.41630325474426755 1101 78-79 0.4270435676072353 1101 80-81 0.32265373672553466 1101 82-83 0.2783644319719336 1101 84-85 0.486982812894464 1101 86-87 0.29254868461388384 1101 88-89 0.35408994534675386 1101 90-91 0.3249869141323849 1101 92-93 0.4022427362685441 1101 94-95 0.0536464998249464 1101 96-97 -0.03349049898104539 1101 98-99 0.11068946045393702 1101 100-101 0.11931380571556005 1102 1 0.4231540995608327 1102 2 0.4756820568569644 1102 3 0.2640774449349408 1102 4 0.1349156489279224 1102 5 0.1519249325762715 1102 6 0.18599573239404776 1102 7 0.11439198876120571 1102 8 0.059718704454120086 1102 9 0.1039437991593104 1102 10-11 0.22174109434435962 1102 12-13 0.25611173767460826 1102 14-15 0.37228666377612285 1102 16-17 0.4441401564316081 1102 18-19 0.2973335022916075 1102 20-21 0.3211085735799273 1102 22-23 0.12492801973307621 1102 24-25 0.2372853226759517 1102 26-27 -0.026567751249721994 1102 28-29 0.07510070426732796 1102 30-31 0.11679249351593768 1102 32-33 0.14772462111048412 1102 34-35 0.4002830399831794 1102 36-37 0.298128898758641 1102 38-39 0.038957398439087854 1102 40-41 0.17967673472572443 1102 42-43 0.04743310922197708 1102 44-45 -0.026739210747500408 1102 46-47 0.06821761111647362 1102 48-49 0.027217893359839707 1102 50-51 -0.10653743043632602 1102 52-53 -0.28878395481124386 1102 54-55 -0.03058139641606772 1102 56-57 -0.17785366227702326 1102 58-59 -0.11354964474595164 1102 60-61 -0.11171396707235459 1102 62-63 -0.1479420581149924 1102 64-65 -0.07709529797503478 1102 66-67 -0.10910762498597393 1102 68-69 -0.17575469230708762 1102 70-71 -0.2840655347005381 1102 72-73 -0.28454929258281325 1102 74-75 -0.8575386537482821 1102 76-77 -0.4650539278180368 1102 78-79 -0.28877651549534633 1102 80-81 -0.30066667478820364 1102 82-83 -0.17823247455674718 1102 84-85 -0.08360568695573178 1102 86-87 -0.20592201949316546 1102 88-89 -0.27534352437697507 1102 90-91 -0.4530662742384308 1102 92-93 -0.3368536088873739 1102 94-95 -0.46254853822221165 1102 96-97 -0.5087140612358283 1102 98-99 -0.7477198712115616 1102 100-101 -0.7289513421276972 1103 1 0.4302340164069385 1103 2 0.4232480976713582 1103 3 0.23421035083222108 1103 4 0.11751876491644708 1103 5 0.009937371844593201 1103 6 0.06543019121317428 1103 7 0.15432868494090712 1103 8 0.14611895063563907 1103 9 -0.06668772577121729 1103 10-11 0.13205045287747907 1103 12-13 0.2751546224621677 1103 14-15 0.2446609036526226 1103 16-17 0.16098194845049107 1103 18-19 0.2579529971291521 1103 20-21 0.17403827742659672 1103 22-23 0.08215829160641164 1103 24-25 0.1111592239683219 1103 26-27 0.188731010149489 1103 28-29 0.19245827701219298 1103 30-31 0.1692058248541244 1103 32-33 0.08463959126351028 1103 34-35 0.11094670715792887 1103 36-37 0.1295099415977674 1103 38-39 0.3171691500561309 1103 40-41 0.4643073821273376 1103 42-43 0.3095303695225837 1103 44-45 0.2560382274598396 1103 46-47 0.29330200352914915 1103 48-49 0.22874004251100644 1103 50-51 0.18321433370960705 1103 52-53 0.2148075732303667 1103 54-55 0.307151670784215 1103 56-57 0.29308009443442984 1103 58-59 0.25454769840421676 1103 60-61 0.256753493766432 1103 62-63 0.26503793033679557 1103 64-65 0.2575654035239481 1103 66-67 0.33695131486101815 1103 68-69 0.279457178511862 1103 70-71 0.15074767760481578 1103 72-73 0.09423614990565099 1103 74-75 0.2678100963647907 1103 76-77 0.13277879555660732 1103 78-79 0.3538746025086219 1103 80-81 0.27928990528377184 1103 82-83 0.2841729353498579 1103 84-85 0.5469636839033747 1103 86-87 0.4191752230539656 1103 88-89 0.28189063635284484 1103 90-91 0.32575822589556935 1103 92-93 0.439804544565213 1103 94-95 0.35025315461392026 1103 96-97 -0.1396252287635349 1103 98-99 -0.10658946517267154 1103 100-101 0.17025516206930646 1104 1 0.260452688366982 1104 2 0.3013846369127826 1104 3 0.09188201205610369 1104 4 0.013520984719654905 1104 5 -0.023141964775753365 1104 6 0.097203792630431 1104 7 0.08805155104835904 1104 8 -0.006970097623735683 1104 9 0.13167410215134367 1104 10-11 -0.0531913242457378 1104 12-13 -0.021548756334439645 1104 14-15 0.13780539363149558 1104 16-17 0.006405471961194564 1104 18-19 0.06552540753661873 1104 20-21 0.0064842230841080095 1104 22-23 0.00802059977485925 1104 24-25 -0.057374798784657344 1104 26-27 0.16201279709107297 1104 28-29 0.128019684452795 1104 30-31 0.040874786028915366 1104 32-33 0.04966781904946771 1104 34-35 0.09078465085176646 1104 36-37 0.07505192576032726 1104 38-39 0.022168776531550805 1104 40-41 0.1945764799054004 1104 42-43 0.14340984914949217 1104 44-45 0.27340872115261305 1104 46-47 0.37338407221498215 1104 48-49 0.20946590748720695 1104 50-51 0.15336518427842805 1104 52-53 0.06371501383996048 1104 54-55 0.06022070879834729 1104 56-57 0.012285661017848781 1104 58-59 0.05067318131813181 1104 60-61 -0.09937417693429751 1104 62-63 -0.17809988956032186 1104 64-65 -0.1559767300484438 1104 66-67 -0.03657486387582054 1104 68-69 -0.0310175178660117 1104 70-71 -0.06644699273306287 1104 72-73 -0.021336890157531485 1104 74-75 -0.10303737754184894 1104 76-77 -0.14358777079202767 1104 78-79 -0.10013571744185867 1104 80-81 -0.04996534006851405 1104 82-83 -0.049526985676376256 1104 84-85 -0.017740911516145985 1104 86-87 0.10896946437219057 1104 88-89 -0.042731837020188124 1104 90-91 -0.13991940104942913 1104 92-93 -0.09445086086903487 1104 94-95 0.009977066590188599 1104 96-97 -0.14643271419608084 1104 98-99 -0.15251164399489525 1104 100-101 -0.09525758045486654 1105 1 0.3024405594426014 1105 2 0.33444025906574026 1105 3 0.08019493584613002 1105 4 0.052022481624412364 1105 5 -0.03466997564444796 1105 6 0.05023187419483577 1105 7 0.0633848966765953 1105 8 0.07334406795175852 1105 9 0.004764549624432846 1105 10-11 0.06054308427709287 1105 12-13 0.03533362445107002 1105 14-15 0.04237041523978746 1105 16-17 0.05124230319517409 1105 18-19 0.08857528182120689 1105 20-21 0.046823808376949216 1105 22-23 0.011542321799346666 1105 24-25 -0.09380268301583072 1105 26-27 0.05296774240948565 1105 28-29 0.09558303395393608 1105 30-31 0.14554861755181747 1105 32-33 0.15149153776661706 1105 34-35 0.1521750356208429 1105 36-37 0.22272181061853047 1105 38-39 0.07882946480036424 1105 40-41 -0.03583965654346599 1105 42-43 0.1554584724274335 1105 44-45 0.06511073567289571 1105 46-47 0.19966012973055314 1105 48-49 0.3045051436047217 1105 50-51 0.14040904328044945 1105 52-53 0.03951979225502811 1105 54-55 0.10784464144685302 1105 56-57 0.36627674106383523 1105 58-59 0.32354170574503627 1105 60-61 0.33841782707383317 1105 62-63 0.3822173804788349 1105 64-65 0.4347600575386181 1105 66-67 0.292429894252642 1105 68-69 0.11420913871882732 1105 70-71 0.23228117612516996 1105 72-73 0.12072190715480247 1105 74-75 0.1449378051911836 1105 76-77 0.28705282452730074 1105 78-79 0.39343596800527436 1105 80-81 0.1668733784334755 1105 82-83 0.31630255160485277 1105 84-85 0.32888552007192473 1105 86-87 0.17264785423135365 1105 88-89 0.42923616241424867 1105 90-91 0.2728417120083755 1105 92-93 0.3495950237186811 1105 94-95 0.6156586961569772 1105 96-97 0.48775350137049855 1105 98-99 0.679017411940773 1105 100-101 0.6459411882103403 1106 1 0.27551895892290545 1106 2 0.30333387517953625 1106 3 0.1530304259587396 1106 4 0.059267521114101385 1106 5 -0.10822541746226477 1106 6 -0.06346389976452826 1106 7 -0.019790543517174797 1106 8 0.03741333095580046 1106 9 0.01350854865984985 1106 10-11 -0.06709803733015463 1106 12-13 0.04754491582010445 1106 14-15 0.113690438796624 1106 16-17 0.045784634550948056 1106 18-19 -0.05950295361428459 1106 20-21 0.0034879856634617568 1106 22-23 -0.03555453600414893 1106 24-25 -0.04468643347413348 1106 26-27 0.015003362281539978 1106 28-29 0.014526335142690527 1106 30-31 0.029055571821864135 1106 32-33 0.08275316956965639 1106 34-35 -0.06839378569251409 1106 36-37 0.11538000519045966 1106 38-39 -0.016114345747553216 1106 40-41 -0.048259079778283365 1106 42-43 -0.26613610064181614 1106 44-45 -0.16538023406986468 1106 46-47 -0.007973552444092036 1106 48-49 -0.09814061226869342 1106 50-51 -0.09110823186946249 1106 52-53 -0.05225580017233966 1106 54-55 -0.13667687672234052 1106 56-57 -0.21531966520628743 1106 58-59 -0.39291471697877967 1106 60-61 -0.14230557367371688 1106 62-63 -0.4681552883417339 1106 64-65 -0.25185189607934433 1106 66-67 -0.34717614390910256 1106 68-69 -0.2731165739123753 1106 70-71 -0.20801020028468997 1106 72-73 -0.1249960103946286 1106 74-75 -0.3360803635928882 1106 76-77 -0.18662936241981498 1106 78-79 -0.05964987910987318 1106 80-81 -0.1557048688952598 1106 82-83 -0.08108290204879509 1106 84-85 -0.0482049235790889 1106 86-87 -0.2652925548472176 1106 88-89 -0.05490911075728988 1106 90-91 -0.16906550588169722 1106 92-93 0.03834786476991425 1106 94-95 -0.006949222778633413 1106 96-97 0.04697644976446114 1106 98-99 -0.0864821578516839 1106 100-101 -0.13253801787834973 1107 1 0.5828640145577992 1107 2 0.5830715637685309 1107 3 0.40844768824435107 1107 4 0.24474438435099444 1107 5 0.024973323151336047 1107 6 0.26105297131216787 1107 7 0.22194044289407344 1107 8 0.23643491810364026 1107 9 0.3428142788868982 1107 10-11 0.33649276671577155 1107 12-13 0.4336929910092877 1107 14-15 0.5949976498749834 1107 16-17 0.5929229769774551 1107 18-19 0.44287076392611624 1107 20-21 0.32887559068235106 1107 22-23 0.433274561777246 1107 24-25 0.4663478734668658 1107 26-27 0.35334983651328145 1107 28-29 0.5229626427952283 1107 30-31 0.4871656590088804 1107 32-33 0.43357633684454555 1107 34-35 0.3842244167064379 1107 36-37 0.42807563035896834 1107 38-39 0.5060725042644165 1107 40-41 0.49464382906722193 1107 42-43 0.3992813344413406 1107 44-45 0.37900719507427283 1107 46-47 0.6380853497423189 1107 48-49 0.5573145707937428 1107 50-51 0.5701193361092081 1107 52-53 0.5274851786713199 1107 54-55 0.5421232549825348 1107 56-57 0.6322403505774687 1107 58-59 0.45423259671765237 1107 60-61 0.5188950094824989 1107 62-63 0.387307966496536 1107 64-65 0.4404464462240796 1107 66-67 0.47428327577045337 1107 68-69 0.5483391767781569 1107 70-71 0.5202534660484517 1107 72-73 0.6922587478698894 1107 74-75 0.7506064848804677 1107 76-77 0.6416484591096712 1107 78-79 0.8442559845736923 1107 80-81 0.95938847610309 1107 82-83 0.7304049156057673 1107 84-85 0.7646498883710215 1107 86-87 0.9271861596905246 1107 88-89 1.054318704345551 1107 90-91 0.8708414958985919 1107 92-93 0.9418258769921444 1107 94-95 1.1749448135045881 1107 96-97 1.131921238581004 1107 98-99 1.223337926762305 1107 100-101 1.336718102882994 1108 1 0.3438949137640108 1108 2 0.29217610176615594 1108 3 0.1379267619237723 1108 4 0.0377319424554301 1108 5 -0.12123359506210107 1108 6 -0.046297072698500585 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-1.3544364427220543 2316 56-57 -1.3562926981608712 2316 58-59 -1.4203518266231896 2316 60-61 -1.330519676371189 2316 62-63 -1.2474940389726648 2316 64-65 -1.1482682342789978 2316 66-67 -0.9826078131380029 2316 68-69 -1.1911188779976953 2316 70-71 -0.9887602994990701 2316 72-73 -1.109064300709381 2316 74-75 -1.0381575080682879 2316 76-77 -1.2119538801223584 2316 78-79 -1.1137785457071878 2316 80-81 -1.0561260002088595 2316 82-83 -0.8045495101500784 2316 84-85 -0.9615127501485041 2316 86-87 -1.0889845625994248 2316 88-89 -1.0501761033865264 2316 90-91 -1.2008037032239045 2316 92-93 -1.2888399567135629 2316 94-95 -1.563021720553536 2316 96-97 -1.5500868046982603 2316 98-99 -2.0092444402061638 2316 100-101 -2.7532620709108393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 36.0 5 74.0 6 148.0 7 358.0 8 895.0 9 1740.0 10 2789.0 11 4402.0 12 6035.0 13 8648.0 14 11531.0 15 15096.0 16 19452.0 17 24429.0 18 20907.0 19 14019.0 20 12399.0 21 12606.0 22 13035.0 23 14749.0 24 17030.0 25 21026.0 26 25401.0 27 32090.0 28 39621.0 29 49633.0 30 60727.0 31 76073.0 32 95141.0 33 121491.0 34 161044.0 35 219904.0 36 309568.0 37 405516.0 38 260057.0 39 15736.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.246464651338975 19.301206156489094 15.04667552966894 22.405653662502985 2 15.869122569314722 20.58047884177506 39.44630396333918 24.104094625571033 3 19.463975028877876 24.62592521466127 29.988324643696213 25.921775112764646 4 13.280063827855889 17.09310936253362 36.50217616679327 33.124650642817215 5 13.823454808009695 37.56898423770178 33.52181342143189 15.08574753285663 6 32.11067285815078 36.225866563358494 16.97806405138403 14.685396527106692 7 29.090246406227532 33.146015621380016 19.909879225933917 17.853858746458535 8 27.25926513306261 34.22774196969255 19.1718022196289 19.341190677615934 9 27.053018476019773 16.545725712604163 19.974891415883377 36.42636439549268 10-11 25.427365737940942 26.685506776994046 26.467608290030697 21.41951919503431 12-13 26.468339582704875 23.97047783986944 27.435684496662265 22.12549808076342 14-15 23.072471383446885 25.338938216906605 26.12485594692876 25.463734452717752 16-17 22.256621125406216 28.811061171398094 26.63112821024805 22.30118949294764 18-19 22.12461710245831 26.42221731264144 29.880876320362088 21.572289264538163 20-21 22.907233307522215 25.47810680453403 30.131345319819836 21.48331456812392 22-23 22.544640031489678 25.48786426955965 30.08749706515931 21.879998633791356 24-25 22.37484911004183 25.82084853777062 30.22227338800323 21.582028964184325 26-27 22.139491825072263 26.265433528883214 29.861833283733308 21.73324136231121 28-29 21.91452427208582 26.232353577626583 30.046889935239722 21.806232215047867 30-31 22.038556175967187 25.964991141260708 30.033992336915837 21.962460345856265 32-33 21.89884444355272 26.04832848099467 30.04081314829717 22.012013927155444 34-35 22.55247444348906 26.076287379382823 29.55932932072227 21.81190885640585 36-37 22.668649774738732 25.889233844173948 29.42147192988071 22.020644451206607 38-39 22.160780735737383 25.97623016991416 29.65474990565632 22.208239188692133 40-41 22.423930030747815 26.033460252961866 29.59789463576869 21.944715080521632 42-43 22.418727133570997 26.074895348315014 29.321892434980274 22.184485083133712 44-45 22.3999682808634 26.322799060941588 29.13768135499948 22.13955130319554 46-47 22.85560467597521 26.528895951359083 28.426997181354665 22.188502191311045 48-49 23.0264732565789 26.781019592780137 27.961980136338727 22.230527014302236 50-51 22.86910902227355 26.88587120728097 27.880137718379526 22.364882052065955 52-53 23.074007266779468 26.91178114439834 27.7657901500075 22.24842143881469 54-55 22.719363831154403 26.944731756606654 27.77854482826164 22.557359583977295 56-57 22.864646940422624 27.17465634407082 27.5309296904726 22.429767025033957 58-59 23.1494901108056 26.74756711104827 27.512138171659828 22.5908046064863 60-61 22.870689737538576 26.842771562061092 28.03614935555067 22.250389344849662 62-63 22.750504580034157 26.889087934267252 28.10397334384293 22.25643414185566 64-65 22.68175509258264 26.810694722156526 27.9968896246766 22.510660560584235 66-67 22.596333099815915 27.18754187370139 27.724767280746615 22.491357745736078 68-69 22.804904816850392 26.790050594448346 27.89313042371268 22.511914164988582 70-71 22.683867768737166 26.899320863306603 27.796118550124127 22.620692817832104 72-73 22.801000758331195 26.982307595851275 27.626853283812913 22.58983836200462 74-75 22.732399196909544 27.347995832642674 27.38888599621766 22.530718974230123 76-77 22.816947697602004 27.218318008534947 27.450190324943684 22.514543968919366 78-79 22.85700362824369 27.079205307378334 27.368217884764267 22.69557317961371 80-81 22.750645651933468 27.47430232444252 27.24661909151738 22.528432932106632 82-83 22.808570266929816 27.52366469399173 27.074378301626727 22.59338673745173 84-85 22.78632285088863 27.303491186281004 27.046541825155003 22.863644137675365 86-87 22.761229504163303 27.244909512800923 27.091386314244097 22.902474668791676 88-89 22.79698983686468 27.33615089139181 26.86310409394897 23.00375517779454 90-91 22.763965420687573 27.34454886535437 27.053696175289517 22.83778953866855 92-93 22.75005684630098 26.958020678677958 27.260189818875265 23.031732656145802 94-95 22.56575295215874 26.99145906725256 27.20983138847724 23.23295659211146 96-97 22.850341826034196 26.83179101554102 27.165805930717525 23.152061227707257 98-99 22.418614183791345 27.043782229700486 26.940112116599735 23.597491469908434 100-101 22.217521617935788 27.483437934386203 26.90764443270222 23.39139601497579 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 377.0 1 276.5 2 239.0 3 316.5 4 292.0 5 279.5 6 457.5 7 915.0 8 1486.0 9 1844.0 10 1864.0 11 1801.0 12 1885.0 13 2075.5 14 2396.0 15 2682.5 16 2884.0 17 3157.5 18 3482.0 19 3805.0 20 4158.0 21 4643.5 22 5267.0 23 6158.0 24 7377.0 25 8857.0 26 11079.5 27 13981.5 28 16862.5 29 20186.0 30 24387.0 31 28849.5 32 33509.5 33 37223.5 34 40898.0 35 44459.0 36 48885.5 37 53785.0 38 56514.5 39 57797.5 40 59771.5 41 64163.0 42 67805.0 43 70283.0 44 72496.5 45 74716.0 46 75685.5 47 76371.5 48 79079.5 49 78607.0 50 75057.0 51 71555.0 52 69249.5 53 69143.0 54 68761.0 55 64666.5 56 58794.0 57 53912.0 58 50237.5 59 45363.5 60 38853.0 61 32710.0 62 27389.0 63 21937.0 64 16498.5 65 12928.0 66 9844.5 67 7722.5 68 5679.5 69 3576.5 70 2653.5 71 2125.0 72 1834.5 73 1714.5 74 1398.5 75 1122.5 76 850.0 77 540.0 78 334.5 79 238.5 80 175.5 81 122.0 82 90.0 83 63.5 84 33.5 85 17.5 86 14.0 87 10.0 88 5.5 89 3.0 90 1.5 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0092609532853765 2 0.01332751826801496 3 0.0054934215083215785 4 0.013518593798739188 5 0.0 6 0.0 7 0.0 8 0.0 9 0.005015732681511007 10-11 3.343821787674004E-4 12-13 1.1942220670264301E-4 14-15 2.38844413405286E-5 16-17 0.0 18-19 2.38844413405286E-5 20-21 0.0 22-23 0.0013614131564101302 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.004418621647997791 34-35 6.209954748537436E-4 36-37 0.0031527462569497754 38-39 1.433066480431716E-4 40-41 0.004466390530678848 42-43 0.01332751826801496 44-45 0.0018868708659017595 46-47 0.0015047198044533018 48-49 0.011655607374177956 50-51 5.015732681511006E-4 52-53 0.002436213016733917 54-55 0.012324371731712759 56-57 0.003654319525100876 58-59 8.120710055779724E-4 60-61 0.007308639050201752 62-63 0.0042036616759330335 64-65 0.01996739296068191 66-67 0.0030810929329281897 68-69 7.16533240215858E-5 70-71 9.553776536211441E-4 72-73 2.38844413405286E-5 74-75 0.0 76-77 9.314932122806154E-4 78-79 0.012013873994285886 80-81 0.017841677681374863 82-83 0.015094966927214076 84-85 0.009506007653530383 86-87 0.011655607374177956 88-89 0.03355764008344268 90-91 0.02870909849131538 92-93 0.0023406752513718027 94-95 0.009506007653530383 96-97 0.0116078384914969 98-99 0.011225687430048441 100-101 0.014402318128338747 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2093413.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.55092154478517 #Duplication Level Percentage of deduplicated Percentage of total 1 76.32806696212259 40.87438325553735 2 13.475727741114357 14.432752780466002 3 4.383521659236534 7.042248733909264 4 1.9464382869314425 4.169342559809269 5 1.023256399111179 2.7398161574501065 6 0.6181631073813346 1.9861922439155073 7 0.38463739166473826 1.4418380748960444 8 0.28585152566614697 1.2246090099523983 9 0.21690374486288988 1.0453855881530443 >10 1.159351127284696 11.632035722240929 >50 0.10890028120983304 4.0432278112743765 >100 0.06434438554820489 6.3106029236863685 >500 0.0036728315281252337 1.3412376439615443 >1k 9.853938246189627E-4 0.8836846324766824 >5k 1.7916251356708414E-4 0.8326428622712 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9162 0.43765850312384613 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8195 0.39146599357126377 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4575 0.2185426382658367 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2332 0.11139703441222538 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.77688826810572E-5 0.0 0.0 0.0 0.0 8 4.77688826810572E-5 0.0 0.0 0.0 0.0 9 4.77688826810572E-5 0.0 0.0 0.0 0.0 10-11 9.55377653621144E-5 0.0 0.0 0.0 0.0 12-13 9.55377653621144E-5 0.0 0.0 0.0 0.0 14-15 9.55377653621144E-5 0.0 0.0 0.0 0.0 16-17 1.1942220670264301E-4 0.0 0.0 0.0 0.0 18-19 1.6719108938370022E-4 0.0 0.0 0.0 0.0 20-21 1.910755307242288E-4 0.0 0.0 2.38844413405286E-5 0.0 22-23 1.910755307242288E-4 0.0 0.0 1.910755307242288E-4 0.0 24-25 1.910755307242288E-4 0.0 0.0 3.343821787674004E-4 0.0 26-27 1.910755307242288E-4 0.0 0.0 6.209954748537436E-4 0.0 28-29 2.3884441340528602E-4 0.0 0.0 0.00119422206702643 0.0 30-31 2.3884441340528602E-4 0.0 0.0 0.0026511729887986747 0.0 32-33 2.6272885474581465E-4 0.0 0.0 0.0047530038267651915 0.0 34-35 3.104977374268718E-4 0.0 0.0 0.007738558994331266 0.0 36-37 4.7768882681057203E-4 0.0 0.0 0.010867420809940513 0.0 38-39 4.7768882681057203E-4 0.0 0.0 0.014259011480295574 0.0 40-41 4.7768882681057203E-4 0.0 0.0 0.01722068220652112 0.0 42-43 5.493421508321578E-4 0.0 0.0 0.02032565958078984 0.0 44-45 6.687643575348008E-4 0.0 0.0 0.0243860146086797 0.0 46-47 8.359554469185011E-4 0.0 0.0 0.030285471619790266 0.0 48-49 8.598398882590296E-4 0.0 0.0 0.03690146187111669 0.0 50-51 8.598398882590296E-4 0.0 0.0 0.042418767820778795 0.0 52-53 8.598398882590296E-4 0.0 0.0 0.05259353983184398 0.0 54-55 8.598398882590296E-4 0.0 0.0 0.07143836404952104 0.0 56-57 8.598398882590296E-4 0.0 0.0 0.10984454572509103 0.0 58-59 8.598398882590296E-4 0.0 0.0 0.15336199784753415 0.0 60-61 8.598398882590296E-4 0.0 0.0 0.19702275661802043 0.0 62-63 8.598398882590296E-4 2.38844413405286E-5 0.0 0.2493535675951186 0.0 64-65 8.598398882590296E-4 4.77688826810572E-5 0.0 0.3150596657229128 0.0 66-67 9.076087709400868E-4 4.77688826810572E-5 0.0 0.4220619629284809 0.0 68-69 9.076087709400868E-4 4.77688826810572E-5 0.0 0.5476224710556398 0.0 70-71 9.076087709400868E-4 4.77688826810572E-5 0.0 0.6533827773115004 0.0 72-73 9.314932122806154E-4 4.77688826810572E-5 0.0 0.7652336161091958 4.77688826810572E-5 74-75 9.553776536211441E-4 4.77688826810572E-5 0.0 0.889767093258712 4.77688826810572E-5 76-77 9.553776536211441E-4 4.77688826810572E-5 0.0 1.0332648168326077 4.77688826810572E-5 78-79 9.553776536211441E-4 7.16533240215858E-5 0.0 1.1570101074178865 4.77688826810572E-5 80-81 9.553776536211441E-4 9.55377653621144E-5 0.0 1.2673801108524692 4.77688826810572E-5 82-83 0.0010509154189832584 9.55377653621144E-5 0.0 1.370847510739639 4.77688826810572E-5 84-85 0.0010509154189832584 9.55377653621144E-5 0.0 1.5621857703186137 4.77688826810572E-5 86-87 0.0010509154189832584 9.55377653621144E-5 0.0 1.8372389967961409 4.77688826810572E-5 88-89 0.0010509154189832584 9.55377653621144E-5 0.0 2.174224579669659 4.77688826810572E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5870 0.0 47.651413 1 GTATCAA 10295 0.0 40.45188 1 ATCAACG 13360 0.0 31.141733 3 TCAACGC 13525 0.0 30.725964 4 TATCAAC 13575 0.0 30.613525 2 CAACGCA 13685 0.0 30.399979 5 AACGCAG 14155 0.0 29.457685 6 ACGCAGA 16240 0.0 25.617226 7 CGCAGAG 16455 0.0 25.25365 8 GTGGTAT 3255 0.0 25.057821 1 TGGTATC 3190 0.0 24.119564 2 GTACATG 10445 0.0 24.023628 1 GCAGAGT 17420 0.0 23.500284 9 TACATGG 11085 0.0 22.536951 2 GAGTACT 10045 0.0 22.52661 12-13 ACATGGG 10940 0.0 21.880554 3 GTAAGGT 860 0.0 21.537779 4 AGTACTT 10365 0.0 21.44171 12-13 GTACTTT 10590 0.0 21.232782 14-15 AGAGTAC 16065 0.0 20.573652 10-11 >>END_MODULE