FastQCFastQC Report
Thu 26 May 2016
SRR936693_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936693_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175207
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73590.33831262955663527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58140.2672849066778472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32870.15111205508257375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53850.047.5697251
GTATCAA94050.039.061441
ATCAACG117650.030.8240813
TCAACGC118050.030.680084
TATCAAC120200.030.2887862
CAACGCA120050.030.005765
AACGCAG124950.028.9431656
GTACATG112100.026.115761
ACGCAGA141450.025.5003667
TACATGG114200.025.1794832
CGCAGAG144000.024.9810688
ACATGGG113500.024.5391373
GGACCGT2452.9649527E-1023.2762366
GTAAGGT6950.023.2520734
GAGTACT74950.023.14588412-13
CGTATAG1252.2485846E-422.8122861
TACACCG2503.8198777E-1022.8107135
GCAGAGT153950.022.7491639
TAAGGTG8900.021.8923025
GTACTTT79850.021.5769814-15
Produced by FastQC (version 0.11.5)