##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936693_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2175207 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42584498854592 31.0 31.0 34.0 30.0 34.0 2 31.47128618103932 31.0 31.0 34.0 28.0 34.0 3 31.517767734289198 33.0 31.0 34.0 28.0 34.0 4 35.14526801357296 37.0 35.0 37.0 32.0 37.0 5 35.11746468267158 37.0 35.0 37.0 32.0 37.0 6 35.24229739974172 37.0 35.0 37.0 32.0 37.0 7 35.10759527713914 37.0 35.0 37.0 32.0 37.0 8 35.055321631458526 37.0 35.0 37.0 32.0 37.0 9 36.55243064223313 39.0 37.0 39.0 32.0 39.0 10-11 36.456909848120205 39.0 37.0 39.0 32.0 39.0 12-13 36.34659666873084 39.0 37.0 39.0 32.0 39.0 14-15 37.4267184686331 40.0 37.0 41.0 32.0 41.0 16-17 37.30161796095728 40.0 37.0 41.0 32.0 41.0 18-19 37.24981783342919 39.5 37.0 41.0 31.5 41.0 20-21 37.1673847592436 39.5 36.5 41.0 31.0 41.0 22-23 37.03263942236302 39.0 36.0 41.0 31.0 41.0 24-25 37.16634072067624 39.0 36.5 41.0 31.0 41.0 26-27 37.151268132182366 39.0 36.5 41.0 31.0 41.0 28-29 37.04920520207962 39.0 36.0 41.0 31.0 41.0 30-31 36.85266965396856 39.0 36.0 41.0 30.0 41.0 32-33 36.67834233707413 39.0 35.5 41.0 30.0 41.0 34-35 36.46939440706103 39.0 35.0 41.0 30.0 41.0 36-37 36.27756277908263 39.0 35.0 40.0 30.0 41.0 38-39 36.07345852601615 39.0 35.0 40.0 29.5 41.0 40-41 35.87453056191893 38.0 35.0 40.0 29.0 41.0 42-43 35.633454884983365 38.0 35.0 40.0 27.5 41.0 44-45 35.36973676528257 38.0 34.5 40.0 27.0 41.0 46-47 35.07449911663579 38.0 34.0 40.0 26.0 41.0 48-49 34.9700065327116 38.0 34.0 40.0 25.0 41.0 50-51 34.058215149178906 37.5 33.0 39.5 23.5 40.5 52-53 34.19651761878295 38.0 33.5 39.5 24.0 40.5 54-55 34.59959442940373 38.0 34.0 40.0 24.5 41.0 56-57 34.51245973371729 38.0 34.0 40.0 24.0 41.0 58-59 34.256696948842105 38.0 34.0 40.0 23.5 41.0 60-61 33.95304584805032 37.5 33.0 40.0 23.0 41.0 62-63 33.62182449762253 37.0 33.0 40.0 22.0 41.0 64-65 33.28087947491893 36.0 33.0 39.5 22.0 41.0 66-67 32.915001882579446 36.0 32.0 39.0 20.5 41.0 68-69 32.5098075723368 35.5 32.0 39.0 19.5 40.5 70-71 32.0827063815076 35.0 31.0 38.5 18.0 40.0 72-73 31.699549973864556 35.0 31.0 37.5 17.5 40.0 74-75 31.279509260497967 35.0 31.0 37.0 14.0 39.0 76-77 30.849566730890437 35.0 30.5 36.5 11.0 39.0 78-79 30.412123535829004 34.0 30.0 36.0 7.5 38.5 80-81 29.95374233348826 34.0 29.5 35.5 7.0 37.0 82-83 29.485503448637303 34.0 29.0 35.0 2.0 37.0 84-85 29.1691517175147 34.0 29.0 35.0 2.0 36.5 86-87 28.838532148894338 34.0 29.0 35.0 2.0 36.0 88-89 28.446600944186002 34.0 29.0 35.0 2.0 36.0 90-91 28.058938988335363 34.0 27.5 35.0 2.0 35.0 92-93 27.698496281043596 34.0 27.0 35.0 2.0 35.0 94-95 27.243300752526082 33.0 26.0 35.0 2.0 35.0 96-97 26.66534173529232 33.0 25.0 35.0 2.0 35.0 98-99 25.95369521153619 33.0 24.5 35.0 2.0 35.0 100-101 23.44886785487542 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3772334175509151 1101 2 0.437084735868428 1101 3 0.45450743181002906 1101 4 0.4601523465936097 1101 5 0.3589328136895773 1101 6 0.3062848022038338 1101 7 0.38402593517531614 1101 8 0.4991890297740653 1101 9 0.5843012736118993 1101 10-11 0.4348726948001982 1101 12-13 0.5978643343394552 1101 14-15 0.9267987517333509 1101 16-17 1.0061546356302387 1101 18-19 0.8821463776693079 1101 20-21 0.8287788797647124 1101 22-23 0.8547009895324038 1101 24-25 0.8663117752229041 1101 26-27 0.821066500118917 1101 28-29 0.81430087609548 1101 30-31 0.7160590312860649 1101 32-33 0.6076056773206062 1101 34-35 0.6264783166150991 1101 36-37 0.6056074577137807 1101 38-39 0.5309198877969976 1101 40-41 0.5549991494009419 1101 42-43 0.4250560260407923 1101 44-45 0.4299797717094336 1101 46-47 0.6327867289894087 1101 48-49 0.6967849309966923 1101 50-51 0.5607906939842593 1101 52-53 0.6933865329047819 1101 54-55 0.7829785489538708 1101 56-57 0.6872980589170865 1101 58-59 0.7561151221736111 1101 60-61 0.5683814447133742 1101 62-63 0.6363669514027706 1101 64-65 0.674953250743215 1101 66-67 0.6477106673930635 1101 68-69 0.7297081117735402 1101 70-71 0.638785638202215 1101 72-73 0.5566768453789912 1101 74-75 0.6034034762475855 1101 76-77 0.6762208440673412 1101 78-79 0.4844575673250233 1101 80-81 0.683379363267651 1101 82-83 0.8465707912976477 1101 84-85 0.8843273790520314 1101 86-87 0.7174434513736152 1101 88-89 0.7329076864031485 1101 90-91 0.5751448339547274 1101 92-93 0.6462663874473726 1101 94-95 0.5465252607720963 1101 96-97 0.3291714368393457 1101 98-99 0.49704416988198474 1101 100-101 0.8881780736810825 1102 1 0.3219847918007517 1102 2 0.45588957978390354 1102 3 0.3841997385325051 1102 4 0.34027331840257347 1102 5 0.47779533827478105 1102 6 0.39247075459679337 1102 7 0.4813461479949197 1102 8 0.39083983162993263 1102 9 0.5328302589364284 1102 10-11 0.5444700648138152 1102 12-13 0.40236185060351914 1102 14-15 0.49348264905545847 1102 16-17 0.4578391948616627 1102 18-19 0.5105567866662355 1102 20-21 0.5761816736524423 1102 22-23 0.5485873113338045 1102 24-25 0.37478894250315165 1102 26-27 0.32345930232779807 1102 28-29 0.41477256528808226 1102 30-31 0.36278415610324544 1102 32-33 0.3925406210898288 1102 34-35 0.2630049947128299 1102 36-37 0.3140149669174619 1102 38-39 0.3499494656879705 1102 40-41 0.26711902433160617 1102 42-43 0.3910461793515978 1102 44-45 0.058800722085145196 1102 46-47 0.10227066035256627 1102 48-49 0.17508281540840898 1102 50-51 0.29520304458186786 1102 52-53 0.30882808946336127 1102 54-55 0.3750241324426966 1102 56-57 0.44598104847782594 1102 58-59 0.5765337640617645 1102 60-61 0.3705633815231053 1102 62-63 0.4016453945920375 1102 64-65 0.48059981007550334 1102 66-67 0.6148684602721843 1102 68-69 0.44433315590082856 1102 70-71 0.28965526097694294 1102 72-73 0.3988404693640639 1102 74-75 0.26460707955587637 1102 76-77 0.3822552507444321 1102 78-79 0.2732897386397646 1102 80-81 0.514954329226601 1102 82-83 0.7089004284452614 1102 84-85 0.6431766339312475 1102 86-87 0.592688673018678 1102 88-89 0.6034829801648094 1102 90-91 0.5638934786165883 1102 92-93 0.5904029740125019 1102 94-95 0.6340347166195919 1102 96-97 0.5795451004129006 1102 98-99 0.692933555377973 1102 100-101 0.8937675827334814 1103 1 0.31888096876021876 1103 2 0.4076155419370835 1103 3 0.3157380648334893 1103 4 0.32946539742060565 1103 5 0.42180697361553143 1103 6 0.3235522978777041 1103 7 0.30705865278970634 1103 8 0.29446932274360194 1103 9 0.4655842226239315 1103 10-11 0.4302447419650761 1103 12-13 0.37365692156630814 1103 14-15 0.3361210584660128 1103 16-17 0.5455699038502857 1103 18-19 0.4743516978029092 1103 20-21 0.5423995486045001 1103 22-23 0.5380890534383909 1103 24-25 0.5989030263798938 1103 26-27 0.37883944872852027 1103 28-29 0.2529712451244208 1103 30-31 0.19935332884330847 1103 32-33 0.4525092951724261 1103 34-35 0.3113968244021308 1103 36-37 0.4478095584019357 1103 38-39 0.4543264635143345 1103 40-41 0.3548450184499998 1103 42-43 0.4989660425404523 1103 44-45 0.6440857719508912 1103 46-47 0.6946511903359465 1103 48-49 0.6150049800932322 1103 50-51 0.7233091051879228 1103 52-53 0.7994411025051704 1103 54-55 0.9689562542941204 1103 56-57 1.0408161079331393 1103 58-59 0.858345393009472 1103 60-61 0.8046203275658073 1103 62-63 0.7458767908329307 1103 64-65 0.9596983109066031 1103 66-67 0.8073263460924096 1103 68-69 0.7695728950646128 1103 70-71 0.9323334670886894 1103 72-73 0.9438791472824839 1103 74-75 0.7794125711809841 1103 76-77 0.7052348888981186 1103 78-79 0.8722606982357242 1103 80-81 0.5302311480477222 1103 82-83 0.6796139721097525 1103 84-85 0.726448993603924 1103 86-87 0.8276410448381384 1103 88-89 0.7679469233945753 1103 90-91 0.9900049892929736 1103 92-93 0.9204593127159946 1103 94-95 0.9490654032325594 1103 96-97 0.8849837431695775 1103 98-99 0.811650391460315 1103 100-101 1.44066831473706 1104 1 0.2422427270465981 1104 2 0.25986263599322257 1104 3 0.26373918152807363 1104 4 0.40619297470094295 1104 5 0.4091583770975902 1104 6 0.30704958227951096 1104 7 0.3505457854225682 1104 8 0.3062946108000446 1104 9 0.45556696461672885 1104 10-11 0.5702350977845825 1104 12-13 0.4446467190385235 1104 14-15 0.4818103635774307 1104 16-17 0.4192910347573502 1104 18-19 0.5852260189033629 1104 20-21 0.6000430692379695 1104 22-23 0.3376098899441544 1104 24-25 0.42078567193087935 1104 26-27 0.09200502408001654 1104 28-29 0.28345040861865556 1104 30-31 0.37080553939100014 1104 32-33 0.1372796114000323 1104 34-35 0.13750539757100455 1104 36-37 0.24449145272201633 1104 38-39 0.5069382598698482 1104 40-41 0.639259261381838 1104 42-43 0.5545502768432797 1104 44-45 0.5117673617179079 1104 46-47 0.5772228872041225 1104 48-49 0.41538440245758323 1104 50-51 0.2283060596572426 1104 52-53 0.3327401736183049 1104 54-55 0.5507216469137859 1104 56-57 0.396795246047958 1104 58-59 0.4286854772691484 1104 60-61 0.42356606636079164 1104 62-63 0.5751321585807574 1104 64-65 0.3768588611324759 1104 66-67 0.5916680038762792 1104 68-69 0.46431174080849047 1104 70-71 0.5096072095383093 1104 72-73 0.5934408446582502 1104 74-75 0.27237866427270774 1104 76-77 0.34837077032545594 1104 78-79 0.38643358286087093 1104 80-81 0.4541306962940048 1104 82-83 0.371946341025037 1104 84-85 0.3685714746962532 1104 86-87 0.5120733071598487 1104 88-89 0.4731517353330581 1104 90-91 0.5798232726288433 1104 92-93 0.6285893568761871 1104 94-95 0.7688331815179268 1104 96-97 0.8926079754420542 1104 98-99 1.1565123274026554 1104 100-101 1.4917306973572337 1105 1 0.3157829068438538 1105 2 0.34133694073178233 1105 3 0.3944625823318866 1105 4 0.43645999914643596 1105 5 0.4589506897641371 1105 6 0.27485618526333155 1105 7 0.47898116534305046 1105 8 0.4872648030912785 1105 9 0.466174757021669 1105 10-11 0.49326409587737174 1105 12-13 0.4576338745703623 1105 14-15 0.2432527934076134 1105 16-17 0.32649698900659985 1105 18-19 0.4415157416173301 1105 20-21 0.41845571219509736 1105 22-23 0.40390240687502654 1105 24-25 0.44907888536961593 1105 26-27 0.4528797181486155 1105 28-29 0.3339578324548498 1105 30-31 0.42913546831891836 1105 32-33 0.33071085766541586 1105 34-35 0.1706286809507347 1105 36-37 0.21841575362829957 1105 38-39 0.2576153272740953 1105 40-41 0.1673834637649918 1105 42-43 0.08981033621015655 1105 44-45 0.05021584208807184 1105 46-47 0.21508242404127742 1105 48-49 0.25276014166003336 1105 50-51 0.3036715030447752 1105 52-53 0.3005342193690552 1105 54-55 0.2687989280328438 1105 56-57 0.41794357989846986 1105 58-59 0.425749689192088 1105 60-61 0.3136125151008642 1105 62-63 0.44679463206174574 1105 64-65 0.4061419566039177 1105 66-67 0.41841753339861754 1105 68-69 0.47885227258033325 1105 70-71 0.4449289016654703 1105 72-73 0.4000842906755473 1105 74-75 0.475463260212571 1105 76-77 0.4329477348162065 1105 78-79 0.5335976573425647 1105 80-81 0.4719579675533083 1105 82-83 0.5235525134538896 1105 84-85 0.5604079523607517 1105 86-87 0.5872652746978169 1105 88-89 0.4042287106137259 1105 90-91 0.47606164649134186 1105 92-93 0.48360242756057303 1105 94-95 0.5075304625328023 1105 96-97 0.6295278963854329 1105 98-99 0.4097998110224417 1105 100-101 0.7588838777416065 1106 1 0.0901955909973502 1106 2 0.1034895961321709 1106 3 0.1048852563054048 1106 4 0.21570100068572629 1106 5 0.3113291324734817 1106 6 0.26860783479671113 1106 7 0.3602784895335276 1106 8 0.3613793827532916 1106 9 0.4418915766065652 1106 10-11 0.42118225361291906 1106 12-13 0.490795745227274 1106 14-15 0.406060303313005 1106 16-17 0.46611185472434613 1106 18-19 0.4087567259441087 1106 20-21 0.24194723953596053 1106 22-23 0.21044462017588472 1106 24-25 0.18703350086645543 1106 26-27 -0.11939986321005591 1106 28-29 0.3132140793709439 1106 30-31 0.23266364603739476 1106 32-33 0.06793998584297611 1106 34-35 0.11141862916563383 1106 36-37 0.0616824198578243 1106 38-39 0.11896016212404703 1106 40-41 0.12163114540218345 1106 42-43 -0.11319999048351548 1106 44-45 0.11216247999069395 1106 46-47 0.09138811679611791 1106 48-49 0.3415709162071394 1106 50-51 0.16282297078112862 1106 52-53 0.33965551601293953 1106 54-55 0.3970775036884362 1106 56-57 0.37915167765247304 1106 58-59 0.3089987904525344 1106 60-61 0.3825406749510947 1106 62-63 0.4124950956523392 1106 64-65 0.3747615819938588 1106 66-67 0.4475155070749324 1106 68-69 0.4855337023999624 1106 70-71 0.56149333141461 1106 72-73 0.4889631981229918 1106 74-75 0.2687948627311769 1106 76-77 0.3716799669852655 1106 78-79 0.4029599415917957 1106 80-81 0.48402107685610574 1106 82-83 0.6519595682929875 1106 84-85 0.3794623373737238 1106 86-87 0.528200607251943 1106 88-89 0.6824129862370221 1106 90-91 0.8481730164277863 1106 92-93 0.8567052592663664 1106 94-95 0.7754209326969459 1106 96-97 0.9283652519846655 1106 98-99 1.118142433653464 1106 100-101 1.5424791351179934 1107 1 0.0722907572528797 1107 2 0.1992071486115634 1107 3 0.05961774561924926 1107 4 0.13409121359337917 1107 5 0.13569390927042235 1107 6 0.1986831645555327 1107 7 0.24762788098324506 1107 8 0.23549170122453944 1107 9 0.19618302836086343 1107 10-11 0.15333475814901476 1107 12-13 0.19081078059861767 1107 14-15 0.09528172591397066 1107 16-17 0.13933462286362897 1107 18-19 0.36810218611141465 1107 20-21 0.35160011783269596 1107 22-23 0.43570973539609525 1107 24-25 0.38768392933206286 1107 26-27 0.11660662323602367 1107 28-29 0.272597340330627 1107 30-31 0.28234875269508564 1107 32-33 0.23079854122411092 1107 34-35 0.325216513000818 1107 36-37 0.34934814872504916 1107 38-39 0.29549198614394356 1107 40-41 0.06413571547550845 1107 42-43 0.1315270113445095 1107 44-45 0.18867977831285287 1107 46-47 0.1806914269562867 1107 48-49 0.1513755922925739 1107 50-51 -0.029037286909847637 1107 52-53 0.24299124481221668 1107 54-55 0.3479305274120392 1107 56-57 0.3242097124869687 1107 58-59 0.31982510727923597 1107 60-61 0.22546691498698124 1107 62-63 0.2092419339770757 1107 64-65 0.34947285189661415 1107 66-67 0.44788162261453834 1107 68-69 0.3251814669715358 1107 70-71 0.43723915378996026 1107 72-73 0.30249486244516177 1107 74-75 0.21170929532853933 1107 76-77 0.15841893934736362 1107 78-79 0.23142164471711268 1107 80-81 0.511600365079925 1107 82-83 0.6465086090872632 1107 84-85 0.4324046830955268 1107 86-87 0.46888776619535477 1107 88-89 0.44512254703428766 1107 90-91 0.4517531337206755 1107 92-93 0.4112110287715751 1107 94-95 0.35554204813461965 1107 96-97 0.48808073518922157 1107 98-99 0.721634207521479 1107 100-101 1.1200935058749586 1108 1 -0.05794550707241086 1108 2 0.23027392893053644 1108 3 0.03480206020605081 1108 4 0.28918717745473543 1108 5 0.21636743865398955 1108 6 0.1880589832835824 1108 7 -0.036285436470144816 1108 8 0.11747763587969473 1108 9 0.28173340971491 1108 10-11 0.11114460667942438 1108 12-13 0.34440481699002135 1108 14-15 0.31775089205987683 1108 16-17 0.3146457068399542 1108 18-19 0.32363975107582377 1108 20-21 0.261276056226869 1108 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-2.1318052397320884 2316 68-69 -2.601899989006693 2316 70-71 -2.6623215192198373 2316 72-73 -2.076194593499917 2316 74-75 -1.612245289768211 2316 76-77 -1.708726206874001 2316 78-79 -2.2981564087168174 2316 80-81 -1.9134569136718333 2316 82-83 -1.7175007067372086 2316 84-85 -1.695678581358294 2316 86-87 -2.390286259703341 2316 88-89 -2.6061155233450357 2316 90-91 -2.4819234060212523 2316 92-93 -2.9697496384378397 2316 94-95 -3.23849842401631 2316 96-97 -4.201526693025897 2316 98-99 -5.4746320050583 2316 100-101 -5.9210446529601555 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4894.0 3 1993.0 4 1947.0 5 2220.0 6 2428.0 7 2852.0 8 3510.0 9 4454.0 10 5408.0 11 6889.0 12 8656.0 13 10367.0 14 11941.0 15 14651.0 16 17797.0 17 21337.0 18 18138.0 19 13609.0 20 13767.0 21 14797.0 22 16105.0 23 18292.0 24 21104.0 25 24627.0 26 28669.0 27 34153.0 28 40888.0 29 48604.0 30 59473.0 31 73503.0 32 92166.0 33 118558.0 34 158773.0 35 216749.0 36 301965.0 37 391497.0 38 304702.0 39 43702.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.47572187319351 18.392262492486594 14.32272205300934 22.80929358131056 2 15.834574130055115 19.69592739706752 40.15044985970337 24.319048613173997 3 19.355446872143308 24.37058448091613 29.469043314161482 26.804925332779085 4 12.859990690332113 16.357371656292948 37.040197158895424 33.74244049447952 5 13.965661978633609 37.262572016697526 33.66404857461562 15.107717430053244 6 32.28800906663184 35.54246626000517 17.50685102887321 14.662673644489784 7 29.23335351033903 32.838168761947514 20.07334556219332 17.855132165520136 8 27.136704571168906 33.859295863006594 19.628228030443797 19.375771535380704 9 27.147504430297946 16.490044995177612 20.16334035956588 36.199110214958566 10-11 25.284989870374368 26.373019065406634 26.88158169809277 21.46040936612623 12-13 26.28705994903826 23.707788683549662 27.48728256170142 22.51786880571066 14-15 23.135746903930436 25.137803668233616 26.001718262843625 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22.549086539931366 26.127897971807634 28.96991421564402 22.35310127261698 42-43 22.475063949201072 26.250227569047667 28.620853416110233 22.653855065641025 44-45 22.437548071331072 26.430646487856258 28.54465869757208 22.58714674324059 46-47 22.69081834886679 26.636897715471285 27.992820880131774 22.679463055530142 48-49 22.807134089435866 26.84245973914922 27.691351100363647 22.659055071051267 50-51 22.7445574532359 26.873581020959225 27.593560958431627 22.788300567373245 52-53 23.007839339401812 26.79547031783771 27.55547453829548 22.641215804464995 54-55 22.644204664626255 26.775027344186412 27.635995661710922 22.94477232947641 56-57 22.7293303522677 27.152557374976293 27.336387201342628 22.78172507141338 58-59 22.888927254741624 26.70218138992026 27.506515617596865 22.90237573774125 60-61 22.70752316592198 26.717409315657008 27.933798383603943 22.64126913481707 62-63 22.60108649476706 26.791070440096586 27.926667120292333 22.68117594484402 64-65 22.422831482245137 26.79521994918185 27.85364795166621 22.928300616906803 66-67 22.48008875292123 27.0978475209733 27.45188910298585 22.97017462311962 68-69 22.552515277704654 26.847193525715586 27.7725116957982 22.82777950078156 70-71 22.48655331251256 26.767241161323007 27.708474803811 23.03773072235343 72-73 22.50971485841765 26.89210731686012 27.616701855851154 22.981475968871077 74-75 22.452568872399432 27.14599488995473 27.45349410551795 22.94794213212789 76-77 22.519394305946253 27.095891903597074 27.405239905041633 22.979473885415036 78-79 22.607090519346922 27.059463125046694 27.370600033331993 22.96284632227439 80-81 22.51347897422552 27.388392852011407 27.265747002877983 22.832381170885093 82-83 22.61347414203319 27.338067313240355 27.232184933254562 22.816273611471892 84-85 22.541269103384156 27.216319027262596 27.098676620635082 23.143735248718166 86-87 22.65445266884281 27.08726035584571 27.167670451933247 23.090616523378234 88-89 22.58393762909171 27.117575322894506 27.029991230096744 23.26849581791704 90-91 22.56324755812716 27.042234613344945 27.295093818810752 23.099424009717147 92-93 22.53829975657733 26.791966040376714 27.41236278162127 23.25737142142469 94-95 22.34662185306687 26.780087680781268 27.473372767462017 23.399917698689844 96-97 22.471224322352878 26.629924405871254 27.432785869969273 23.4660654018066 98-99 22.15084359543194 26.774748535379096 27.290219682215028 23.78418818697394 100-101 22.115898025043496 26.831409640554515 27.4219236130625 23.630768721339493 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 200.0 1 160.5 2 170.0 3 242.5 4 231.0 5 239.0 6 413.0 7 812.5 8 1310.0 9 1622.5 10 1582.0 11 1495.5 12 1594.0 13 1706.0 14 1905.5 15 2172.0 16 2369.5 17 2596.0 18 2749.0 19 2932.0 20 3277.0 21 3790.0 22 4502.0 23 5465.5 24 6713.5 25 8322.0 26 10448.5 27 13356.5 28 16451.0 29 19670.5 30 23943.5 31 28911.0 32 34474.0 33 38341.5 34 41646.0 35 45612.5 36 50506.5 37 55878.5 38 58624.5 39 59846.5 40 62264.5 41 66811.5 42 70865.5 43 73978.5 44 76730.0 45 80008.0 46 80647.0 47 80216.5 48 83064.0 49 83188.5 50 79342.0 51 75480.5 52 73259.0 53 73347.0 54 73248.0 55 69128.5 56 62967.0 57 57078.5 58 52604.0 59 47091.5 60 40261.5 61 34052.0 62 28583.0 63 22859.0 64 17417.5 65 13839.5 66 10360.0 67 8024.0 68 5756.0 69 3581.0 70 2703.0 71 2137.0 72 1781.5 73 1642.0 74 1350.0 75 1086.0 76 807.5 77 489.5 78 315.5 79 195.0 80 122.5 81 95.0 82 75.5 83 52.0 84 31.0 85 21.5 86 15.5 87 10.5 88 8.0 89 6.5 90 5.5 91 5.5 92 5.0 93 3.0 94 2.5 95 2.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.034939203487300285 2 0.0243195245326077 3 0.02257256435824269 4 0.05162727041610293 5 0.05663828775836047 6 0.049696419697067916 7 0.05125948932676293 8 0.04463942971864287 9 0.04477734762714537 10-11 0.05233984627669919 12-13 0.04721389734402289 14-15 0.04220288000176535 16-17 0.05052392714808291 18-19 0.054086806451064194 20-21 0.012711433900313856 22-23 0.021377275817887675 24-25 0.00606838797411005 26-27 0.011746008540796347 28-29 0.006895895425125057 30-31 0.009792171503677581 32-33 0.0037007972114837806 34-35 0.008711814553741322 36-37 0.009125568279248826 38-39 0.011654063268461346 40-41 0.0019998096732862665 42-43 0.0019768233552025162 44-45 0.0024825223530450208 46-47 4.597263616750038E-4 48-49 7.815348148475065E-4 50-51 3.4479477125625287E-4 52-53 0.004022605664656283 54-55 0.00717173124213006 56-57 0.01698688906389139 58-59 0.011056418998283841 60-61 0.011263295861037593 62-63 0.006390196427282552 64-65 0.0 66-67 0.008275074510150068 68-69 0.0178143965149064 70-71 0.006252278518780052 72-73 0.0027353718519662726 74-75 0.0036548245753162804 76-77 0.006826936470873807 78-79 0.0054937300220162955 80-81 0.011286282179121344 82-83 0.006987840697460058 84-85 0.01820516392233015 86-87 0.00491907206992254 88-89 0.007102772287878809 90-91 0.0038387151199862812 92-93 0.017883355469157647 94-95 0.012504557037560104 96-97 0.01087252845361384 98-99 0.02287138649333144 100-101 0.038157288019025314 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2175207.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.28686439288758 #Duplication Level Percentage of deduplicated Percentage of total 1 77.3672851167488 42.7739460069556 2 12.992643816976972 14.366450736285904 3 4.15815631472908 6.896742728905673 4 1.844650626946722 4.079397962570339 5 0.9655555329126488 2.6691268905971954 6 0.5899172886115944 1.9568806283093483 7 0.37382576313747345 1.4467357991204446 8 0.2549298863936072 1.127541924699008 9 0.19209803493949057 0.9558448207055816 >10 1.0918246483669756 11.279418906193769 >50 0.10241854412680305 3.939968081014824 >100 0.06284737720009761 6.203851724178179 >500 0.002927102431447822 1.0834770788238575 >1k 7.526834823722745E-4 0.6115336424923827 >5k 1.6726299608272766E-4 0.6090830691480537 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7359 0.33831262955663527 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5814 0.2672849066778472 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3287 0.15111205508257375 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.298631808375019E-5 0.0 12-13 0.0 0.0 0.0 4.597263616750038E-5 0.0 14-15 0.0 0.0 0.0 4.597263616750038E-5 0.0 16-17 0.0 0.0 0.0 4.597263616750038E-5 0.0 18-19 2.298631808375019E-5 0.0 0.0 4.597263616750038E-5 0.0 20-21 4.597263616750038E-5 0.0 0.0 4.597263616750038E-5 0.0 22-23 4.597263616750038E-5 0.0 0.0 1.1493159041875095E-4 0.0 24-25 9.194527233500076E-5 0.0 0.0 2.988221350887525E-4 0.0 26-27 9.194527233500076E-5 0.0 0.0 6.436169063450053E-4 0.0 28-29 1.1493159041875095E-4 0.0 0.0 0.0014941106754437624 0.0 30-31 1.3791790850250114E-4 0.0 0.0 0.0032410708498087767 0.0 32-33 1.3791790850250114E-4 0.0 0.0 0.005815538475188798 0.0 34-35 1.3791790850250114E-4 0.0 0.0 0.008183129237815067 0.0 36-37 1.3791790850250114E-4 0.0 0.0 0.011194336906786343 0.0 38-39 1.3791790850250114E-4 0.0 0.0 0.015193956253358875 4.597263616750038E-5 40-41 1.3791790850250114E-4 0.0 0.0 0.018251136558497652 4.597263616750038E-5 42-43 1.6090422658625132E-4 0.0 0.0 0.022089851678483933 4.597263616750038E-5 44-45 1.8389054467000151E-4 0.0 0.0 0.025606758345297714 4.597263616750038E-5 46-47 1.8389054467000151E-4 0.0 0.0 0.03229577690766902 4.597263616750038E-5 48-49 1.8389054467000151E-4 0.0 0.0 0.04034098823698158 4.597263616750038E-5 50-51 1.8389054467000151E-4 0.0 0.0 0.04804140479503789 4.597263616750038E-5 52-53 1.8389054467000151E-4 0.0 0.0 0.05810941211572048 4.597263616750038E-5 54-55 1.8389054467000151E-4 0.0 0.0 0.07948668793360816 4.597263616750038E-5 56-57 1.8389054467000151E-4 0.0 0.0 0.12079310153010725 4.597263616750038E-5 58-59 1.8389054467000151E-4 0.0 0.0 0.1674323409220364 6.895895425125057E-5 60-61 1.8389054467000151E-4 0.0 0.0 0.21292226440977802 9.194527233500076E-5 62-63 1.8389054467000151E-4 0.0 0.0 0.26551496018539844 9.194527233500076E-5 64-65 1.8389054467000151E-4 0.0 0.0 0.33086506249750025 9.194527233500076E-5 66-67 1.8389054467000151E-4 0.0 0.0 0.4365101804104161 9.194527233500076E-5 68-69 1.8389054467000151E-4 0.0 0.0 0.5632797246422985 9.194527233500076E-5 70-71 1.8389054467000151E-4 0.0 0.0 0.6717751459975994 9.194527233500076E-5 72-73 2.068768627537517E-4 0.0 0.0 0.7887755050438878 1.3791790850250114E-4 74-75 2.298631808375019E-4 0.0 0.0 0.9098214560729163 1.3791790850250114E-4 76-77 2.298631808375019E-4 0.0 0.0 1.0530721903708473 1.3791790850250114E-4 78-79 2.5284949892125205E-4 0.0 0.0 1.1781867197006997 1.3791790850250114E-4 80-81 4.137537255075034E-4 0.0 0.0 1.294474502886392 1.3791790850250114E-4 82-83 4.137537255075034E-4 0.0 0.0 1.3989013459408692 1.3791790850250114E-4 84-85 4.597263616750038E-4 0.0 0.0 1.5910669651210205 1.3791790850250114E-4 86-87 4.597263616750038E-4 0.0 0.0 1.8701668392939155 1.3791790850250114E-4 88-89 4.597263616750038E-4 0.0 0.0 2.2017904502881795 1.3791790850250114E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5385 0.0 47.569725 1 GTATCAA 9405 0.0 39.06144 1 ATCAACG 11765 0.0 30.824081 3 TCAACGC 11805 0.0 30.68008 4 TATCAAC 12020 0.0 30.288786 2 CAACGCA 12005 0.0 30.00576 5 AACGCAG 12495 0.0 28.943165 6 GTACATG 11210 0.0 26.11576 1 ACGCAGA 14145 0.0 25.500366 7 TACATGG 11420 0.0 25.179483 2 CGCAGAG 14400 0.0 24.981068 8 ACATGGG 11350 0.0 24.539137 3 GGACCGT 245 2.9649527E-10 23.276236 6 GTAAGGT 695 0.0 23.252073 4 GAGTACT 7495 0.0 23.145884 12-13 CGTATAG 125 2.2485846E-4 22.812286 1 TACACCG 250 3.8198777E-10 22.810713 5 GCAGAGT 15395 0.0 22.749163 9 TAAGGTG 890 0.0 21.892302 5 GTACTTT 7985 0.0 21.57698 14-15 >>END_MODULE