FastQCFastQC Report
Thu 26 May 2016
SRR936693_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936693_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175207
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92960.4273616258130835No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82440.3789984125648731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46710.2147381835383943No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23560.10831153081063088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61350.050.9676321
GTATCAA103500.038.751211
ATCAACG136700.029.187993
TATCAAC139000.028.9448972
TCAACGC138050.028.9005644
CAACGCA140150.028.467525
AACGCAG143700.027.7985826
GTGGTAT31100.024.6007651
TGGTATC30150.024.262572
ACGCAGA164500.024.254757
CGCAGAG166550.023.9847288
GTACATG108250.023.924991
TAAGGTG10950.022.9892715
GTAAGGT9600.022.7588354
TACATGG111950.022.6579482
GAGTACT104750.022.53594612-13
ACATGGG111600.021.877233
GCAGAGT181600.021.8144179
TATCCCG1100.002879343521.5894095
GTACTTT110750.021.03578414-15
Produced by FastQC (version 0.11.5)