##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936693_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2175207 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.911911831839454 31.0 31.0 34.0 30.0 34.0 2 32.05597995960844 31.0 31.0 34.0 30.0 34.0 3 32.15734272646235 33.0 31.0 34.0 30.0 34.0 4 35.90395166988705 37.0 35.0 37.0 35.0 37.0 5 35.689046605679366 37.0 35.0 37.0 33.0 37.0 6 35.79182762835905 37.0 35.0 37.0 35.0 37.0 7 35.73782771019034 37.0 35.0 37.0 33.0 37.0 8 35.709673608075 37.0 35.0 37.0 33.0 37.0 9 37.3481025024285 39.0 37.0 39.0 34.0 39.0 10-11 37.220678077994414 39.0 37.0 39.0 33.0 39.0 12-13 37.120283724721375 39.0 37.0 39.0 33.0 39.0 14-15 38.23623751670531 40.0 38.0 41.0 33.0 41.0 16-17 38.160817568167076 40.0 37.0 41.0 33.0 41.0 18-19 38.30661035938189 40.0 38.0 41.0 33.5 41.0 20-21 38.33842572224161 40.0 38.0 41.0 34.0 41.0 22-23 38.24950315073462 40.0 38.0 41.0 33.5 41.0 24-25 38.131018565129665 40.0 37.0 41.0 33.0 41.0 26-27 37.921347025823295 40.0 37.0 41.0 33.0 41.0 28-29 37.831465465125845 40.0 37.0 41.0 33.0 41.0 30-31 37.78733909002683 40.0 37.0 41.0 33.0 41.0 32-33 37.62979178533354 40.0 36.5 41.0 33.0 41.0 34-35 37.45764311166707 40.0 36.0 41.0 32.5 41.0 36-37 37.186332381240035 39.0 36.0 41.0 31.5 41.0 38-39 37.01032039709324 39.0 36.0 41.0 31.0 41.0 40-41 36.77541057012046 39.0 35.0 41.0 31.0 41.0 42-43 36.50619389327085 39.0 35.0 41.0 30.0 41.0 44-45 36.230432322073256 39.0 35.0 40.0 30.0 41.0 46-47 35.831210087131936 39.0 35.0 40.0 29.0 41.0 48-49 35.44171451268775 38.0 35.0 40.0 27.5 41.0 50-51 35.102318308096656 38.0 34.5 40.0 26.5 41.0 52-53 34.812728397803056 38.0 34.0 40.0 26.0 41.0 54-55 34.52802009188091 38.0 34.0 40.0 25.5 41.0 56-57 34.21200212209689 38.0 33.5 40.0 24.5 41.0 58-59 33.869522073071664 37.0 33.0 40.0 23.5 41.0 60-61 33.51266638071687 37.0 33.0 39.5 23.0 41.0 62-63 33.109913447317886 36.0 32.0 39.0 22.0 40.5 64-65 32.701401751649385 36.0 31.5 39.0 21.5 40.0 66-67 32.252336904027985 35.0 31.0 38.5 19.5 40.0 68-69 31.97450748365558 35.0 31.0 38.0 19.0 40.0 70-71 31.555593329738272 35.0 31.0 37.5 18.0 39.5 72-73 31.125303476864502 34.5 30.0 37.0 16.0 39.0 74-75 30.603326028281444 34.0 30.0 36.0 12.0 39.0 76-77 29.19042003818487 32.5 28.0 35.0 10.0 37.0 78-79 29.965381225786786 34.0 29.5 35.0 8.0 37.5 80-81 29.776704010239023 34.0 29.0 35.0 7.0 37.0 82-83 29.447571886261862 34.0 29.0 35.0 4.5 36.5 84-85 29.037359432918336 34.0 29.0 35.0 2.0 36.0 86-87 28.64431316191976 34.0 29.0 35.0 2.0 36.0 88-89 28.251247812277178 33.0 28.5 35.0 2.0 35.0 90-91 27.784046070098157 33.0 27.0 35.0 2.0 35.0 92-93 27.300222001860053 33.0 26.0 35.0 2.0 35.0 94-95 26.709979785831877 32.5 25.0 34.0 2.0 35.0 96-97 25.952126165463795 32.0 24.5 34.0 2.0 35.0 98-99 25.00482413857624 32.0 20.5 34.0 2.0 35.0 100-101 21.824440386593093 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2801422427196911 1101 2 0.3027818593585181 1101 3 0.24847629480770905 1101 4 0.11497728863156453 1101 5 0.08871785633225926 1101 6 0.13269500691361458 1101 7 0.24971103575321507 1101 8 0.2169962647368351 1101 9 0.24379401610232776 1101 10-11 0.22514362299414614 1101 12-13 0.20128813653110456 1101 14-15 0.38976963967559186 1101 16-17 0.37605260619186964 1101 18-19 0.35689823094532613 1101 20-21 0.2607185273463628 1101 22-23 0.13941770876541426 1101 24-25 0.050427494330975264 1101 26-27 0.2097140718035604 1101 28-29 0.10531762004291068 1101 30-31 0.18919357052940455 1101 32-33 0.3138070900789529 1101 34-35 0.16160897471028335 1101 36-37 0.2213994488030977 1101 38-39 0.36590871742559017 1101 40-41 0.38244466507772756 1101 42-43 0.5250146441541332 1101 44-45 0.40119318642304336 1101 46-47 0.36639476491372136 1101 48-49 0.41727530642867805 1101 50-51 0.31406320323762316 1101 52-53 0.20846339023412241 1101 54-55 0.3852640629593864 1101 56-57 0.3416732025120268 1101 58-59 0.22841891953369498 1101 60-61 0.29756064939744675 1101 62-63 0.3060408187210939 1101 64-65 0.27588022392173883 1101 66-67 0.04767230894862706 1101 68-69 -0.08281093360914227 1101 70-71 0.004263580167979342 1101 72-73 0.11355219857409793 1101 74-75 0.09416996254593002 1101 76-77 -0.09235926972276332 1101 78-79 -0.1917298673451313 1101 80-81 0.07724967212753597 1101 82-83 0.1438189311250575 1101 84-85 0.08875400650313026 1101 86-87 0.11068924366908561 1101 88-89 -0.14309490958158477 1101 90-91 -0.19734654155834974 1101 92-93 -0.23641654011949598 1101 94-95 -0.08294860642034863 1101 96-97 -0.03163117654523262 1101 98-99 0.11884729043874742 1101 100-101 0.8496104159044044 1102 1 0.28521467451150784 1102 2 0.36524119921428877 1102 3 0.27814716827586494 1102 4 0.1402370092427958 1102 5 0.10545722795967549 1102 6 0.15829104050078513 1102 7 0.23887904725152254 1102 8 0.2370543236404643 1102 9 0.2547662663418748 1102 10-11 0.3664812787004408 1102 12-13 0.26201075239676186 1102 14-15 0.36420055525378103 1102 16-17 0.3680830603247074 1102 18-19 0.30808824935271417 1102 20-21 0.14277195918861452 1102 22-23 0.15983178032727352 1102 24-25 0.2374187697356902 1102 26-27 0.11978833172589987 1102 28-29 0.08798442119274341 1102 30-31 0.21868635886971077 1102 32-33 0.30021052696432093 1102 34-35 0.3479539429386378 1102 36-37 0.5509001170163188 1102 38-39 0.4614931518398322 1102 40-41 0.5017383763087508 1102 42-43 0.5337327779480745 1102 44-45 0.6074392464865923 1102 46-47 0.7159296948143634 1102 48-49 0.7108238968770166 1102 50-51 0.5258365213575473 1102 52-53 0.4344967567679916 1102 54-55 0.5700061074397027 1102 56-57 0.5343326281008132 1102 58-59 0.29730744769951656 1102 60-61 0.3772083560392261 1102 62-63 0.3353126554249073 1102 64-65 0.6156017176934867 1102 66-67 0.34949051082467264 1102 68-69 0.3293475526987919 1102 70-71 0.43189136018714436 1102 72-73 0.36281511526177823 1102 74-75 0.14327543937279685 1102 76-77 0.10174973728717163 1102 78-79 0.19652675805201625 1102 80-81 0.4536751411375697 1102 82-83 0.5391077127075903 1102 84-85 0.44465445534069303 1102 86-87 0.44691081308761227 1102 88-89 0.26088505662952244 1102 90-91 0.404348103910408 1102 92-93 0.25922226541784354 1102 94-95 0.34840572309702367 1102 96-97 0.6236934111798149 1102 98-99 0.6067028303046023 1102 100-101 0.8487097464304014 1103 1 0.09718357235066222 1103 2 0.10919258011187338 1103 3 0.11861593825413053 1103 4 0.08034249447486275 1103 5 -0.073461708237744 1103 6 0.08569754222691017 1103 7 0.11750933749573989 1103 8 0.15100890106023002 1103 9 0.21104823156370855 1103 10-11 0.04492912744100863 1103 12-13 0.11853309608682139 1103 14-15 0.19550145986956835 1103 16-17 0.3078852755145789 1103 18-19 0.1452418262610351 1103 20-21 0.11658636911062814 1103 22-23 0.10161216327063016 1103 24-25 0.173424395614731 1103 26-27 0.16327477847183047 1103 28-29 0.23106714718686305 1103 30-31 0.13462517743511881 1103 32-33 0.13030594315151234 1103 34-35 0.13504774488212234 1103 36-37 0.2622811744258584 1103 38-39 0.3927367587954649 1103 40-41 0.4067856848370752 1103 42-43 0.32033719234520674 1103 44-45 0.2943772179736115 1103 46-47 0.2989570410616551 1103 48-49 0.3055541104046924 1103 50-51 0.42109567939360915 1103 52-53 0.3004626658588947 1103 54-55 0.2564093806784129 1103 56-57 0.17820505490045235 1103 58-59 0.3076724106198512 1103 60-61 0.1888910852298693 1103 62-63 0.19215816750776327 1103 64-65 0.07949284414790014 1103 66-67 0.21444280273107097 1103 68-69 0.3027607191736621 1103 70-71 0.1957449275058991 1103 72-73 0.0485177907508465 1103 74-75 0.06631693023074448 1103 76-77 -0.06991852764058848 1103 78-79 -0.03809229641672118 1103 80-81 0.1639135217794312 1103 82-83 0.24255851823118135 1103 84-85 0.02713623516424235 1103 86-87 0.11588824564099554 1103 88-89 0.22293994091542046 1103 90-91 0.12479841397306046 1103 92-93 0.2571385325192388 1103 94-95 0.4651382381206801 1103 96-97 0.4220361742535914 1103 98-99 0.5676697782652234 1103 100-101 0.9718032606868903 1104 1 0.13714905633624852 1104 2 0.25980168624861477 1104 3 0.18582572455939328 1104 4 0.0596892281431991 1104 5 -0.05837617958437136 1104 6 0.03362962689266169 1104 7 0.07668755179957998 1104 8 -0.015234944429849406 1104 9 0.08657231663553233 1104 10-11 0.18711099638166928 1104 12-13 0.08769176823544456 1104 14-15 0.11615421861277753 1104 16-17 0.27157036764188547 1104 18-19 0.15242166484790687 1104 20-21 0.12584528997758326 1104 22-23 0.08490844264731123 1104 24-25 0.13413070493913182 1104 26-27 0.15105357142650178 1104 28-29 0.24690136879997482 1104 30-31 0.11751462467129414 1104 32-33 0.03845806740915947 1104 34-35 0.13109134672973966 1104 36-37 0.3986217155151479 1104 38-39 0.3006805179955023 1104 40-41 0.30227322354069486 1104 42-43 0.2194169344902761 1104 44-45 0.2267425801379659 1104 46-47 0.3381245960081003 1104 48-49 0.27931508700486063 1104 50-51 0.16393106846015826 1104 52-53 0.28225115375291665 1104 54-55 0.28282123794453895 1104 56-57 0.3155879288662433 1104 58-59 0.06583925494248177 1104 60-61 0.24292108370710963 1104 62-63 0.23445830455664662 1104 64-65 0.30145848041002665 1104 66-67 0.018997393868581014 1104 68-69 0.1506882355433916 1104 70-71 0.014819847560723076 1104 72-73 -0.0402183140154051 1104 74-75 0.17222424458027064 1104 76-77 0.22563525135540274 1104 78-79 0.23939437938653896 1104 80-81 0.22594133762634172 1104 82-83 -0.0032447404866537966 1104 84-85 -0.028833766358520307 1104 86-87 -0.053071959424734416 1104 88-89 -0.0415428264567943 1104 90-91 -0.018192804746803404 1104 92-93 -0.2214793041387999 1104 94-95 -0.013005764721818025 1104 96-97 0.03368177599969613 1104 98-99 0.1687390133294322 1104 100-101 0.7296071792697063 1105 1 0.0934305377846556 1105 2 0.07879800800250791 1105 3 0.06882617029465621 1105 4 0.01505003186564835 1105 5 -0.026394860282401567 1105 6 -0.0785704598731698 1105 7 0.06451596530201442 1105 8 0.05904043810998161 1105 9 -0.19260715840886888 1105 10-11 -0.014407014969322063 1105 12-13 -0.13632117743470928 1105 14-15 0.0306307177975782 1105 16-17 0.10089282771360786 1105 18-19 0.022785813445182157 1105 20-21 0.1675686037179105 1105 22-23 0.10165290834913776 1105 24-25 0.091781668925357 1105 26-27 0.06838325999968475 1105 28-29 0.0910140170404361 1105 30-31 0.049824348174048794 1105 32-33 0.10171312918330955 1105 34-35 0.09316431709020634 1105 36-37 -0.06831676408856424 1105 38-39 0.3205313641111758 1105 40-41 0.29302031007658513 1105 42-43 0.11849863798776994 1105 44-45 0.22445641958546503 1105 46-47 0.14666046355849716 1105 48-49 0.0661097332704017 1105 50-51 0.11392984064180922 1105 52-53 0.0867693578341786 1105 54-55 0.16075339837649238 1105 56-57 -0.019996776833146157 1105 58-59 -0.21039596751960232 1105 60-61 -0.13184097998020405 1105 62-63 0.011522490434948907 1105 64-65 0.04290160972459489 1105 66-67 0.04180895649438554 1105 68-69 0.06634269620744249 1105 70-71 0.012429229432317612 1105 72-73 -0.04131460697137612 1105 74-75 -0.284696446540071 1105 76-77 -0.2476670479390819 1105 78-79 -0.14469649926259365 1105 80-81 0.002459981489817409 1105 82-83 0.13902796615331425 1105 84-85 -0.15648343555449173 1105 86-87 -0.1737089255376958 1105 88-89 -0.1439264125346753 1105 90-91 -0.131160464011586 1105 92-93 -0.0187971907732134 1105 94-95 0.06228042857251026 1105 96-97 0.10773689536968334 1105 98-99 0.4337223968544883 1105 100-101 0.5920635266068643 1106 1 0.06490283136143304 1106 2 0.0855092523695049 1106 3 0.12294520881955862 1106 4 0.06180620911200663 1106 5 -0.015537447176953378 1106 6 -0.03309313204346864 1106 7 0.026132655547101535 1106 8 0.03783174201591066 1106 9 -0.002524767802647432 1106 10-11 0.042380579228257886 1106 12-13 0.009116233736378376 1106 14-15 0.2115566613306683 1106 16-17 0.13518232348506132 1106 18-19 0.08865147626662662 1106 20-21 0.14873031782283164 1106 22-23 0.11126794619209335 1106 24-25 0.05524071377791273 1106 26-27 0.01151902909615643 1106 28-29 0.012166424088718486 1106 30-31 0.09989578014114642 1106 32-33 0.04907928543038764 1106 34-35 0.19158060111977449 1106 36-37 0.06915032720829117 1106 38-39 0.346484569000836 1106 40-41 0.3967825166235741 1106 42-43 0.3581560637157395 1106 44-45 0.35395575239532207 1106 46-47 0.3443183105834322 1106 48-49 0.2442006309608047 1106 50-51 0.3620816460714593 1106 52-53 0.28187913684178767 1106 54-55 0.2498720516687598 1106 56-57 0.17870386216124956 1106 58-59 0.21649374201498972 1106 60-61 0.27581773176905955 1106 62-63 0.17228396422027714 1106 64-65 0.24013170508197135 1106 66-67 0.23405059653628513 1106 68-69 0.33656341401879786 1106 70-71 0.41855438741868056 1106 72-73 0.085601822954807 1106 74-75 0.28737371579859783 1106 76-77 0.036587305599564246 1106 78-79 0.13910858695575 1106 80-81 0.2502348531745646 1106 82-83 0.4913693199755578 1106 84-85 0.2314614056886768 1106 86-87 0.43799808946670993 1106 88-89 0.4454817799701729 1106 90-91 0.6418613868755152 1106 92-93 0.667401214691516 1106 94-95 0.7845277979253709 1106 96-97 0.7317218688378091 1106 98-99 0.9135072116395548 1106 100-101 0.977498310819211 1107 1 -0.038156569398317686 1107 2 0.03371961845106597 1107 3 -0.014279312596926275 1107 4 0.029200689686646797 1107 5 -0.13322787822490767 1107 6 -0.005449285163010131 1107 7 -0.03554719467681622 1107 8 -0.12004305496409984 1107 9 -0.08092955737540564 1107 10-11 -0.0722509607335553 1107 12-13 -0.13588930471679106 1107 14-15 -0.08133067362003032 1107 16-17 -0.04348673185189966 1107 18-19 -0.12795003712420083 1107 20-21 -0.16794178536228088 1107 22-23 -0.17685733837566175 1107 24-25 -0.05416922010043379 1107 26-27 -0.1307988305465244 1107 28-29 -0.2798472453573666 1107 30-31 -0.34481312827390553 1107 32-33 -0.17586516014666387 1107 34-35 -0.24347254084811 1107 36-37 -0.07580381915637702 1107 38-39 -0.2164643684995866 1107 40-41 -0.19252928809667935 1107 42-43 -0.4092582091838892 1107 44-45 -0.38829508516830913 1107 46-47 -0.212565086289203 1107 48-49 -0.36358981503757093 1107 50-51 -0.32008546910797975 1107 52-53 -0.3660039338397425 1107 54-55 -0.28207853717414366 1107 56-57 -0.5081463039923406 1107 58-59 -0.1864257533401812 1107 60-61 -0.09064013861074471 1107 62-63 -0.10529872182758027 1107 64-65 -0.07366414005880273 1107 66-67 -0.0768362865038128 1107 68-69 -4.223066415676158E-6 1107 70-71 -0.044450953960698314 1107 72-73 0.04134049291056385 1107 74-75 0.013704898309320868 1107 76-77 -0.09724873579952487 1107 78-79 -0.17383313016860313 1107 80-81 0.21138073535161794 1107 82-83 0.11657743669761089 1107 84-85 0.1638481052462808 1107 86-87 0.04062511711819994 1107 88-89 -0.03495949543705379 1107 90-91 0.017575354650553265 1107 92-93 -0.012672840883293901 1107 94-95 0.2945645453922907 1107 96-97 0.3560486749511753 1107 98-99 0.6729705763008234 1107 100-101 0.7261090255175198 1108 1 0.008049543945453763 1108 2 0.041826061159930816 1108 3 0.01242004654001505 1108 4 -0.07803579863130494 1108 5 -0.060461856430507055 1108 6 0.006976206771071247 1108 7 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-0.1807561879775612 2316 90-91 -0.42152272770240984 2316 92-93 -0.39191669921315864 2316 94-95 -0.350867033947587 2316 96-97 -0.16222511126753858 2316 98-99 -0.4116090618797621 2316 100-101 -0.5507868700922174 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 10.0 4 28.0 5 74.0 6 156.0 7 362.0 8 875.0 9 1758.0 10 2852.0 11 4409.0 12 6478.0 13 8991.0 14 11730.0 15 15810.0 16 20054.0 17 25333.0 18 22123.0 19 14932.0 20 13406.0 21 13841.0 22 14186.0 23 16168.0 24 18650.0 25 22396.0 26 27256.0 27 33579.0 28 42021.0 29 52588.0 30 64674.0 31 80145.0 32 100762.0 33 127249.0 34 169360.0 35 229764.0 36 324739.0 37 419774.0 38 254975.0 39 13698.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.218400002575436 19.316397096934267 15.053640306642183 22.411562593848117 2 16.00581680289167 20.502207277164313 39.38254168356261 24.109434236381407 3 19.516586257088132 24.604641411898413 29.889283963966946 25.98948836704651 4 13.263788546579544 17.011272103211073 36.51059928935042 33.214340060858966 5 13.853930337280108 37.54265709511102 33.52012118453748 15.083291383071403 6 32.12667145396152 36.16165303268386 17.031843479364213 14.679832033990406 7 29.083117147011755 33.17868138526586 19.866614993423614 17.871586474298766 8 27.23887887451631 34.20465270661597 19.198632589909835 19.357835828957885 9 26.975902124476654 16.561548494348262 20.011897915152417 36.45065146602267 10-11 25.39883178370322 26.66287850316052 26.483331505912222 21.45495820722404 12-13 26.473598119884922 23.913265348342623 27.44898597177437 22.164150559998088 14-15 23.07555623392777 25.3263983055815 26.077774710288963 25.520270750201767 16-17 22.37924674204579 28.73415387897228 26.584681063914108 22.301918315067827 18-19 22.151914737310058 26.484766737142717 29.76245479165891 21.600863733888314 20-21 22.94800516640604 25.54435636340734 30.046549603451446 21.46108886673518 22-23 22.5810025687736 25.50830110394051 29.99677035623775 21.91392597104814 24-25 22.403965992902698 25.836374059681138 30.125648620605162 21.634011326811002 26-27 22.174099540621356 26.28558897197018 29.806304227092674 21.734007260315792 28-29 21.900503477817505 26.223189955638233 29.986009652098712 21.89029691444555 30-31 22.082425362498736 25.94753077906196 29.996643955902503 21.9733999025368 32-33 21.910582934902536 26.12320706141964 29.95781997057742 22.008390033100405 34-35 22.55659251572586 26.116591697335238 29.496659885825316 21.830155901113585 36-37 22.734488167399213 25.880476490319637 29.32998979973972 22.05504554254143 38-39 22.192076773882242 26.022081272711095 29.54817919269815 22.237662760708513 40-41 22.47115619787749 26.0186818097394 29.537060194081917 21.973101798301187 42-43 22.475997565276877 26.116979803199342 29.168451946399315 22.23857068512447 44-45 22.400899973151596 26.31410056013858 29.0943353549322 22.19066411177762 46-47 22.83895279851527 26.574137541852338 28.354864617482384 22.232045042150013 48-49 23.036843456627572 26.78801088364945 27.883639563357583 22.291506096365396 50-51 22.92626548031383 26.87292373695626 27.804615889612915 22.396194893116988 52-53 23.12917601566241 26.852078103316813 27.707584484616998 22.31116139640378 54-55 22.76311397705508 26.897272888601194 27.737938377709387 22.601674756634342 56-57 22.86026838778608 27.18109612865365 27.4653493158132 22.49328616774708 58-59 23.16744211598938 26.707660777297438 27.514785486604502 22.610111620108675 60-61 22.862031940015868 26.86739535062274 27.97986859046342 22.290704118897978 62-63 22.789973617080555 26.892716433843972 28.015501947858045 22.301808001217427 64-65 22.709087694865794 26.786002053802072 27.942351932111887 22.562558319220244 66-67 22.642216824885857 27.1492915191479 27.628040771497282 22.58045088446896 68-69 22.828031363570535 26.766085783181275 27.87636169709006 22.529521156158125 70-71 22.73626352660797 26.89116213724371 27.72094656890723 22.651627767241088 72-73 22.85019762709566 26.978517894475523 27.56950278884645 22.601781689582367 74-75 22.801153622817935 27.30773987488167 27.3037169992014 22.587389503098994 76-77 22.904863299509433 27.11572159657879 27.42117341033686 22.558241693574914 78-79 22.862512025212432 27.072445511564318 27.31978847119753 22.74525399202572 80-81 22.759516885358313 27.472484528833114 27.173417063028754 22.594581522779812 82-83 22.867169341682082 27.47675792775171 27.002939869564408 22.653132861001797 84-85 22.8511857623464 27.305601754420245 26.949338586785988 22.893873896447367 86-87 22.80748333659751 27.195321098639802 27.037812981868637 22.959382582894047 88-89 22.80885139987623 27.34269036284505 26.762500586212827 23.085957651065897 90-91 22.79180042797488 27.309767740404823 26.95995758873766 22.93847424288264 92-93 22.78735115647528 26.944924735206676 27.170560508131025 23.097163600187017 94-95 22.587544930716415 26.987393309302853 27.145474034143323 23.279587725837406 96-97 22.877074194251332 26.834811983548963 27.04884005108538 23.239273771114323 98-99 22.41284983621631 27.090397103614734 26.85321533245216 23.643537727716797 100-101 22.285999592662595 27.430542305851546 26.831075890269002 23.452382211216857 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 368.0 1 285.0 2 245.5 3 305.0 4 293.0 5 283.0 6 474.5 7 939.0 8 1457.5 9 1844.0 10 1889.5 11 1747.5 12 1879.5 13 2129.0 14 2365.0 15 2641.5 16 2890.0 17 3205.0 18 3544.5 19 3803.5 20 4222.5 21 4782.0 22 5437.5 23 6339.5 24 7467.5 25 9101.5 26 11546.0 27 14390.5 28 17400.5 29 20904.0 30 25131.5 31 29850.5 32 34548.5 33 38217.5 34 42073.0 35 45782.0 36 50369.0 37 55511.5 38 58269.0 39 59693.0 40 62131.0 41 66757.5 42 70480.0 43 73059.0 44 75393.0 45 77928.5 46 78509.5 47 78580.0 48 81704.0 49 81932.0 50 78591.5 51 74896.5 52 72253.0 53 72222.0 54 71816.5 55 67394.0 56 61499.0 57 56307.0 58 52283.5 59 47482.5 60 40842.5 61 34293.5 62 28608.5 63 22912.0 64 17421.0 65 13717.0 66 10506.5 67 8261.5 68 6012.0 69 3746.0 70 2770.5 71 2257.5 72 1946.5 73 1774.0 74 1500.0 75 1248.0 76 914.5 77 567.5 78 364.5 79 254.0 80 194.5 81 150.0 82 108.5 83 70.0 84 44.0 85 28.0 86 13.0 87 4.5 88 3.0 89 4.5 90 3.5 91 1.5 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03751367111268031 2 0.005930470065607549 3 0.004275455163577535 4 6.436169063450054E-4 5 5.516716340100047E-4 6 2.298631808375019E-4 7 0.0 8 0.0 9 0.0016550149020300138 10-11 4.82712679758754E-4 12-13 9.65425359517508E-4 14-15 0.0030112076689712748 16-17 0.0031721118955575266 18-19 0.0 20-21 2.2986318083750193E-5 22-23 0.0020457823094537668 24-25 6.436169063450054E-4 26-27 0.004367400435912536 28-29 0.006252278518780052 30-31 0.0012412611765225104 32-33 3.218084531725027E-4 34-35 0.009493349368588828 36-37 0.01206781699396885 38-39 0.009033623006913825 40-41 0.0019768233552025162 42-43 5.056989978425042E-4 44-45 0.001425151721192512 46-47 0.0 48-49 8.045211329312567E-4 50-51 0.0011493159041875095 52-53 0.0028732897604687737 54-55 0.003907674074237533 56-57 0.0015400833116112629 58-59 7.585484967637563E-4 60-61 0.004528304662498787 62-63 0.005769565839021298 64-65 0.009953075730263833 66-67 0.002758358170050023 68-69 0.0033100298040600277 70-71 1.6090422658625135E-4 72-73 0.0011263295861037592 74-75 0.006712004880455055 76-77 0.0031491255774737763 78-79 0.004114550936991284 80-81 0.005171921568843793 82-83 0.005079976296508791 84-85 0.005953456383691299 86-87 0.003723783529567531 88-89 0.010527733682357586 90-91 0.014389435120427618 92-93 9.194527233500076E-4 94-95 0.0024135633987937702 96-97 0.0011493159041875095 98-99 0.003769756165735031 100-101 0.00487309943375504 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2175207.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.80629134529371 #Duplication Level Percentage of deduplicated Percentage of total 1 75.79750897719592 40.02585342297323 2 13.6574071178722 14.423940385752948 3 4.544946445735404 7.200052984867826 4 2.0207640659190043 4.268362240200769 5 1.0528256356955894 2.7797908627167676 6 0.6478607878910525 2.0526675309939866 7 0.40622265927970524 1.5015778467888865 8 0.2825538525530874 1.1936496846922795 9 0.2166530409362866 1.0296579240472854 >10 1.189636613615663 11.754860976199126 >50 0.11078511283270302 4.062037228170266 >100 0.0677729097017138 6.563137149278001 >500 0.00392801956425275 1.4519229243850489 >1k 9.601825601506673E-4 0.8839956139514384 >5k 1.7457864730012135E-4 0.8084932249821789 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9296 0.4273616258130835 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8244 0.3789984125648731 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4671 0.2147381835383943 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2356 0.10831153081063088 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.3791790850250114E-4 0.0 0.0 0.0 0.0 7 1.3791790850250114E-4 0.0 0.0 0.0 0.0 8 1.3791790850250114E-4 0.0 0.0 0.0 0.0 9 1.3791790850250114E-4 0.0 0.0 0.0 0.0 10-11 1.3791790850250114E-4 0.0 0.0 2.298631808375019E-5 0.0 12-13 1.8389054467000151E-4 0.0 0.0 4.597263616750038E-5 0.0 14-15 1.8389054467000151E-4 0.0 0.0 4.597263616750038E-5 0.0 16-17 2.068768627537517E-4 0.0 0.0 4.597263616750038E-5 0.0 18-19 2.298631808375019E-4 0.0 0.0 6.895895425125057E-5 0.0 20-21 2.298631808375019E-4 0.0 0.0 9.194527233500076E-5 0.0 22-23 2.298631808375019E-4 0.0 0.0 1.3791790850250114E-4 0.0 24-25 2.298631808375019E-4 0.0 0.0 2.7583581700500227E-4 0.0 26-27 2.298631808375019E-4 0.0 0.0 6.436169063450053E-4 2.298631808375019E-5 28-29 3.2180845317250265E-4 0.0 0.0 0.0014941106754437624 4.597263616750038E-5 30-31 3.2180845317250265E-4 0.0 0.0 0.0032410708498087767 4.597263616750038E-5 32-33 3.2180845317250265E-4 0.0 0.0 0.005792552157105048 4.597263616750038E-5 34-35 3.6778108934000303E-4 0.0 0.0 0.008229101873982568 4.597263616750038E-5 36-37 4.597263616750038E-4 0.0 0.0 0.011240309542953842 4.597263616750038E-5 38-39 4.597263616750038E-4 0.0 0.0 0.015193956253358875 4.597263616750038E-5 40-41 4.597263616750038E-4 0.0 0.0 0.018228150240413903 4.597263616750038E-5 42-43 4.82712679758754E-4 0.0 0.0 0.022250755905070183 4.597263616750038E-5 44-45 5.516716340100045E-4 0.0 0.0 0.02574467625380021 4.597263616750038E-5 46-47 5.516716340100045E-4 0.0 0.0 0.03241070849808777 4.597263616750038E-5 48-49 5.516716340100045E-4 0.0 0.0 0.040157097692311586 4.597263616750038E-5 50-51 5.516716340100045E-4 0.0 0.0 0.047673623705697894 4.597263616750038E-5 52-53 5.516716340100045E-4 0.0 0.0 0.05762669943596173 4.597263616750038E-5 54-55 5.516716340100045E-4 0.0 0.0 0.07875112575492815 4.597263616750038E-5 56-57 5.516716340100045E-4 0.0 0.0 0.11968975826208725 4.597263616750038E-5 58-59 5.516716340100045E-4 0.0 0.0 0.16632899765401638 4.597263616750038E-5 60-61 5.516716340100045E-4 0.0 0.0 0.21133620846199924 4.597263616750038E-5 62-63 5.516716340100045E-4 4.597263616750038E-5 0.0 0.2638369589652847 4.597263616750038E-5 64-65 5.516716340100045E-4 4.597263616750038E-5 0.0 0.32907212968696775 4.597263616750038E-5 66-67 5.97644270177505E-4 4.597263616750038E-5 0.0 0.43474023391796734 4.597263616750038E-5 68-69 5.97644270177505E-4 4.597263616750038E-5 0.0 0.5621993676923622 4.597263616750038E-5 70-71 5.97644270177505E-4 4.597263616750038E-5 0.0 0.6709706248646681 4.597263616750038E-5 72-73 6.666032244287555E-4 4.597263616750038E-5 0.0 0.7889134229523902 4.597263616750038E-5 74-75 7.355621786800061E-4 4.597263616750038E-5 0.0 0.9106489635239313 4.597263616750038E-5 76-77 7.355621786800061E-4 4.597263616750038E-5 0.0 1.0547731779090448 4.597263616750038E-5 78-79 7.355621786800061E-4 4.597263616750038E-5 0.0 1.1803934062367398 4.597263616750038E-5 80-81 7.815348148475065E-4 4.597263616750038E-5 0.0 1.2963593809692595 4.597263616750038E-5 82-83 7.815348148475065E-4 4.597263616750038E-5 0.0 1.400211566071643 9.194527233500076E-5 84-85 7.815348148475065E-4 4.597263616750038E-5 0.0 1.592676007386883 9.194527233500076E-5 86-87 7.815348148475065E-4 4.597263616750038E-5 0.0 1.871408100470438 9.194527233500076E-5 88-89 7.815348148475065E-4 4.597263616750038E-5 0.0 2.2020662861051843 9.194527233500076E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6135 0.0 50.967632 1 GTATCAA 10350 0.0 38.75121 1 ATCAACG 13670 0.0 29.18799 3 TATCAAC 13900 0.0 28.944897 2 TCAACGC 13805 0.0 28.900564 4 CAACGCA 14015 0.0 28.46752 5 AACGCAG 14370 0.0 27.798582 6 GTGGTAT 3110 0.0 24.600765 1 TGGTATC 3015 0.0 24.26257 2 ACGCAGA 16450 0.0 24.25475 7 CGCAGAG 16655 0.0 23.984728 8 GTACATG 10825 0.0 23.92499 1 TAAGGTG 1095 0.0 22.989271 5 GTAAGGT 960 0.0 22.758835 4 TACATGG 11195 0.0 22.657948 2 GAGTACT 10475 0.0 22.535946 12-13 ACATGGG 11160 0.0 21.87723 3 GCAGAGT 18160 0.0 21.814417 9 TATCCCG 110 0.0028793435 21.589409 5 GTACTTT 11075 0.0 21.035784 14-15 >>END_MODULE