FastQCFastQC Report
Thu 26 May 2016
SRR936692_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936692_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190266
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72190.32959467023640054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57350.26184034267983886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32480.14829249050115373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52150.046.739541
GTATCAA91550.040.48161
ATCAACG114950.031.8304543
TCAACGC116300.031.5426854
CAACGCA118350.030.9977365
TATCAAC118200.030.9912032
AACGCAG123300.029.9838586
GTACATG111800.026.4769331
ACGCAGA140200.026.1322847
CGCAGAG142100.025.7224568
TACATGG114150.025.43122
ACATGGG115300.023.9446163
GCAGAGT151450.023.6966869
GAGTACT76100.023.35702112-13
GTCCGTA1252.2523172E-422.806711
CATGGGG51100.021.5738754
GTACTTT83300.021.16313714-15
GTATAGG6850.020.1154081
AGTACTT80950.020.1082512-13
GTGGTAT30050.019.9226671
Produced by FastQC (version 0.11.5)