##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936692_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2190266 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.392934465494147 31.0 31.0 34.0 30.0 34.0 2 31.445213503747947 31.0 31.0 34.0 28.0 34.0 3 31.402148414850068 33.0 31.0 34.0 28.0 34.0 4 35.062327132868795 37.0 35.0 37.0 32.0 37.0 5 35.09614357342898 37.0 35.0 37.0 32.0 37.0 6 35.15698275917172 37.0 35.0 37.0 32.0 37.0 7 35.03345073155498 37.0 35.0 37.0 32.0 37.0 8 35.0104644823962 37.0 35.0 37.0 32.0 37.0 9 36.51184787601141 39.0 37.0 39.0 32.0 39.0 10-11 36.38518015620021 39.0 37.0 39.0 32.0 39.0 12-13 36.28852203339686 39.0 36.0 39.0 32.0 39.0 14-15 37.29192367502395 40.0 37.0 41.0 32.0 41.0 16-17 37.204234782441944 39.5 37.0 41.0 31.5 41.0 18-19 37.07995558530334 39.0 36.0 41.0 31.0 41.0 20-21 37.07279937687934 39.0 36.0 41.0 31.0 41.0 22-23 37.07180155287075 39.0 36.0 41.0 31.0 41.0 24-25 37.183826530658834 39.0 37.0 41.0 31.0 41.0 26-27 37.1144676034783 39.0 36.0 41.0 31.0 41.0 28-29 37.00052893118918 39.0 36.0 41.0 30.5 41.0 30-31 36.796892477899945 39.0 36.0 41.0 30.0 41.0 32-33 36.63109731877316 39.0 35.5 41.0 30.0 41.0 34-35 36.44809100812412 39.0 35.0 41.0 30.0 41.0 36-37 36.240897680920945 39.0 35.0 40.0 30.0 41.0 38-39 36.047356576781084 39.0 35.0 40.0 29.0 41.0 40-41 35.795787360987205 38.0 35.0 40.0 28.0 41.0 42-43 35.51767935949332 38.0 35.0 40.0 27.0 41.0 44-45 35.364691092314814 38.0 35.0 40.0 27.0 41.0 46-47 35.20670320408571 38.0 34.5 40.0 26.0 41.0 48-49 34.9731932103224 38.0 34.0 40.0 25.0 41.0 50-51 33.993970595352344 37.5 33.0 39.5 23.5 40.5 52-53 34.09473050305306 37.5 33.5 39.5 24.0 40.5 54-55 34.520794962803606 38.0 34.0 40.0 24.0 41.0 56-57 34.38057614919832 38.0 34.0 40.0 23.5 41.0 58-59 34.15660928855216 38.0 33.5 40.0 23.0 41.0 60-61 33.87480333438952 37.0 33.0 40.0 23.0 41.0 62-63 33.53660742576473 37.0 33.0 40.0 22.0 41.0 64-65 33.18288098340567 36.0 32.5 39.5 21.5 41.0 66-67 32.83685566045403 36.0 32.0 39.0 20.0 41.0 68-69 32.40995340292001 35.0 31.5 39.0 18.5 40.0 70-71 32.02637236755719 35.0 31.0 38.0 18.0 40.0 72-73 31.622007098681166 35.0 31.0 37.5 16.0 40.0 74-75 31.21191284528911 35.0 31.0 37.0 13.0 39.0 76-77 30.754002710173104 35.0 30.5 36.5 9.0 39.0 78-79 30.293329212068308 34.0 30.0 36.0 7.0 38.5 80-81 29.864408706522404 34.0 29.5 35.5 6.0 37.0 82-83 29.47584882384149 34.0 29.0 35.0 2.0 37.0 84-85 29.112512818077803 34.0 29.0 35.0 2.0 36.5 86-87 28.756315671247236 34.0 29.0 35.0 2.0 36.0 88-89 28.41111901476807 34.0 28.5 35.0 2.0 36.0 90-91 28.072336647694847 34.0 27.5 35.0 2.0 35.0 92-93 27.654771612215136 34.0 27.0 35.0 2.0 35.0 94-95 27.176474683896842 33.0 26.0 35.0 2.0 35.0 96-97 26.589206288186 33.0 25.0 35.0 2.0 35.0 98-99 25.85390975342721 33.0 23.5 35.0 2.0 35.0 100-101 23.242494062365026 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3829351626685913 1101 2 0.3846049609298845 1101 3 0.5724950586236268 1101 4 0.4867400969384761 1101 5 0.36209131888745816 1101 6 0.3670840346621276 1101 7 0.4472035609529357 1101 8 0.3911310289337493 1101 9 0.6859981719185626 1101 10-11 0.6472780194003391 1101 12-13 0.6212887406166345 1101 14-15 0.8344146562076631 1101 16-17 0.8553001294787848 1101 18-19 0.9817738603252835 1101 20-21 0.9133737376579774 1101 22-23 0.7615420826694006 1101 24-25 0.5669069538055567 1101 26-27 0.6045624449845874 1101 28-29 0.6457895256858919 1101 30-31 0.4817154841049387 1101 32-33 0.5321555841937737 1101 34-35 0.4077689296383298 1101 36-37 0.5278184922219609 1101 38-39 0.5864657290362274 1101 40-41 0.5510940445937962 1101 42-43 0.493836537747427 1101 44-45 0.5180017410994466 1101 46-47 0.6084038989645535 1101 48-49 0.5401886423163091 1101 50-51 0.5343663902978761 1101 52-53 0.5816650191637436 1101 54-55 0.44697862751309714 1101 56-57 0.5598873437270626 1101 58-59 0.47135734580540856 1101 60-61 0.43952073968670646 1101 62-63 0.625985952395169 1101 64-65 0.48393273803171866 1101 66-67 0.45911978167158196 1101 68-69 0.40168516440514423 1101 70-71 0.44866361851481784 1101 72-73 0.5100425653993668 1101 74-75 0.33311767530708636 1101 76-77 0.3905601474625122 1101 78-79 0.3742216239402154 1101 80-81 0.5166454047987514 1101 82-83 0.4803393265751197 1101 84-85 0.3995395090233629 1101 86-87 0.4001916487186037 1101 88-89 0.44152629012467415 1101 90-91 0.40775732470395454 1101 92-93 0.4281778757926631 1101 94-95 0.4586952497604848 1101 96-97 0.5756147998258392 1101 98-99 0.4924498660100909 1101 100-101 0.8312965062588873 1102 1 0.28702970445098686 1102 2 0.42576273972424517 1102 3 0.5755371971076109 1102 4 0.35848398300005613 1102 5 0.35260626270191153 1102 6 0.3389388470410637 1102 7 0.42187959032543176 1102 8 0.3457775440361601 1102 9 0.545146829704116 1102 10-11 0.5339489743368588 1102 12-13 0.4487043015995198 1102 14-15 0.5920754956132086 1102 16-17 0.5083974457707754 1102 18-19 0.5792711998057669 1102 20-21 0.5682749207218407 1102 22-23 0.3163616254054702 1102 24-25 0.49917003690837447 1102 26-27 0.35641970236818565 1102 28-29 0.23753397043405045 1102 30-31 0.17564437832304236 1102 32-33 0.43256429646027783 1102 34-35 0.17206221966244328 1102 36-37 0.3647023638117659 1102 38-39 0.27722940852194 1102 40-41 0.40338565074858934 1102 42-43 0.28153218693071125 1102 44-45 0.490398945023351 1102 46-47 0.46341407096493015 1102 48-49 0.681888151843566 1102 50-51 0.5458330566842022 1102 52-53 0.3720837397294403 1102 54-55 0.7095130310415954 1102 56-57 0.6217928193660711 1102 58-59 0.44179404045946313 1102 60-61 0.4667791625625739 1102 62-63 0.3999580366895543 1102 64-65 0.5886893984917592 1102 66-67 0.4503676261476599 1102 68-69 0.3792047131786376 1102 70-71 0.22776007515037477 1102 72-73 0.5196172817683191 1102 74-75 0.46079257499130577 1102 76-77 0.5142031173081563 1102 78-79 0.32766223210358447 1102 80-81 0.5570307092994504 1102 82-83 0.3748673370471849 1102 84-85 0.4985836743981906 1102 86-87 0.3478849940707107 1102 88-89 0.4771208572882628 1102 90-91 0.6091932693363127 1102 92-93 0.659072972777345 1102 94-95 0.6190744898085789 1102 96-97 0.4860080821304642 1102 98-99 0.4164631609482399 1102 100-101 1.0650825058738178 1103 1 0.37153463304456835 1103 2 0.5074684443131368 1103 3 0.5373423908350041 1103 4 0.45930659044663713 1103 5 0.5038445418093787 1103 6 0.5300036691068826 1103 7 0.5738104995365987 1103 8 0.27826077271578953 1103 9 0.44582897675760336 1103 10-11 0.4777880607639915 1103 12-13 0.34883874047865504 1103 14-15 0.6531639107819345 1103 16-17 0.8592411129164645 1103 18-19 0.8522414036075006 1103 20-21 0.8817430818887573 1103 22-23 0.5838499115328304 1103 24-25 0.6458127776769018 1103 26-27 0.6125639865786567 1103 28-29 0.4406567962151158 1103 30-31 0.4231387421235766 1103 32-33 0.5994856554879036 1103 34-35 0.5479262133177016 1103 36-37 0.5092377306904368 1103 38-39 0.3102923561185449 1103 40-41 0.4741649594538302 1103 42-43 0.5108071030507091 1103 44-45 0.47391668852183244 1103 46-47 0.5261857020838647 1103 48-49 0.5910827852706433 1103 50-51 0.5832107466317638 1103 52-53 0.73887381103291 1103 54-55 0.6717512745815668 1103 56-57 0.7351676314667586 1103 58-59 0.5887252211067633 1103 60-61 0.5558905493390753 1103 62-63 0.6787535092598418 1103 64-65 0.6812800035571911 1103 66-67 0.5567730818265062 1103 68-69 0.6733133949537873 1103 70-71 0.6560555436647171 1103 72-73 0.6291676525126206 1103 74-75 0.6427918985849743 1103 76-77 0.61730516418611 1103 78-79 0.506529900442338 1103 80-81 0.8593575096541279 1103 82-83 0.6545847564446987 1103 84-85 0.6989403477020417 1103 86-87 0.7321847231961769 1103 88-89 0.7212765567916257 1103 90-91 0.7688462242148937 1103 92-93 0.6910587557644803 1103 94-95 0.7847151071354368 1103 96-97 1.0393661837499089 1103 98-99 1.4202473548796775 1103 100-101 1.6884294754923133 1104 1 0.2805362143746777 1104 2 0.40330904933481193 1104 3 0.40552165470035106 1104 4 0.5076939369669731 1104 5 0.4954165878156971 1104 6 0.3877846068208015 1104 7 0.39192237460958523 1104 8 0.44977799153613063 1104 9 0.539870375218598 1104 10-11 0.5200342850238258 1104 12-13 0.6575524758565194 1104 14-15 0.593189821111288 1104 16-17 0.5942596069301871 1104 18-19 0.6042643577334346 1104 20-21 0.7496092733606687 1104 22-23 0.505757140166331 1104 24-25 0.6338389280122954 1104 26-27 0.39835891948341384 1104 28-29 0.35417913645463983 1104 30-31 0.4511446080776196 1104 32-33 0.48793720362363047 1104 34-35 0.4930067669574356 1104 36-37 0.5211546434385639 1104 38-39 0.43590954906727575 1104 40-41 0.5752913748926716 1104 42-43 0.5771239329580382 1104 44-45 0.6124872719107017 1104 46-47 0.7948897210882109 1104 48-49 0.7326865094933837 1104 50-51 0.5348585622864732 1104 52-53 0.5616924174365536 1104 54-55 0.7118993815339394 1104 56-57 0.8589504234208789 1104 58-59 0.749374424538324 1104 60-61 0.7213521319208311 1104 62-63 0.7302512566225161 1104 64-65 0.6443505259767832 1104 66-67 0.7184384398580974 1104 68-69 0.5896475801365142 1104 70-71 0.7406317039681092 1104 72-73 0.8014777945097364 1104 74-75 0.6331977862169857 1104 76-77 0.7475794097961597 1104 78-79 0.5929159449944272 1104 80-81 0.7937308584029026 1104 82-83 0.7280141846690213 1104 84-85 0.700637971061596 1104 86-87 0.6514468852763855 1104 88-89 0.6351314860446884 1104 90-91 0.7795774619686178 1104 92-93 0.7326935130261134 1104 94-95 0.8044175383579031 1104 96-97 0.8983121507774676 1104 98-99 0.9137626755877477 1104 100-101 1.3434780541339677 1105 1 0.34918959336295075 1105 2 0.2522671057106294 1105 3 0.5284312214383888 1105 4 0.45506365110306035 1105 5 0.4966567965611759 1105 6 0.34869127513884024 1105 7 0.46609964671575455 1105 8 0.4275464504530788 1105 9 0.4532768803069871 1105 10-11 0.6233670641616698 1105 12-13 0.6019976869006669 1105 14-15 0.8426011847216515 1105 16-17 0.819239531366641 1105 18-19 0.7598249017963568 1105 20-21 0.6353663593783168 1105 22-23 0.5449115567566523 1105 24-25 0.4793782488558165 1105 26-27 0.533227666021098 1105 28-29 0.6790732727746231 1105 30-31 0.4598503013256945 1105 32-33 0.561796093783208 1105 34-35 0.4123809142087467 1105 36-37 0.3337352065236274 1105 38-39 0.28475115707784227 1105 40-41 0.385533378827688 1105 42-43 0.21978731573593535 1105 44-45 0.2550061038999516 1105 46-47 0.504387061286387 1105 48-49 0.5438054100054188 1105 50-51 0.5783082744301566 1105 52-53 0.7678072018690827 1105 54-55 0.4889081663178416 1105 56-57 0.48871698146971454 1105 58-59 0.5338542469983238 1105 60-61 0.5181694230895388 1105 62-63 0.7230156398466789 1105 64-65 0.8018851390754094 1105 66-67 0.6520563088260829 1105 68-69 0.6255032433974534 1105 70-71 0.6773543017493999 1105 72-73 0.6233223971953237 1105 74-75 0.5676913738161069 1105 76-77 0.7217889576197543 1105 78-79 0.8125611207675156 1105 80-81 0.7330893625327839 1105 82-83 0.5586373449699593 1105 84-85 0.6945792905090826 1105 86-87 0.8163135384359386 1105 88-89 0.6969637169328529 1105 90-91 0.7258755818996647 1105 92-93 0.7779193992957758 1105 94-95 0.7730237658549264 1105 96-97 0.9675427232524392 1105 98-99 1.423935921138309 1105 100-101 1.7729603233558606 1106 1 0.1923949001570513 1106 2 0.10448059576146562 1106 3 0.10526795199575645 1106 4 0.20038539038323222 1106 5 0.2790035483883315 1106 6 0.24930242748531128 1106 7 0.2590030903131293 1106 8 0.39528624505911125 1106 9 0.4462168438086067 1106 10-11 0.46130371109634183 1106 12-13 0.49264380221244153 1106 14-15 0.314482562476293 1106 16-17 0.2400927733691205 1106 18-19 0.5337753043224751 1106 20-21 0.21094085729242096 1106 22-23 -0.03192427694648359 1106 24-25 -0.22194776806029637 1106 26-27 -0.2531258717639133 1106 28-29 -0.14478536362668848 1106 30-31 -0.15266364924184472 1106 32-33 -0.07028371835094305 1106 34-35 0.0441282624798518 1106 36-37 -0.08271143190528818 1106 38-39 0.060026213301632936 1106 40-41 0.14769669526548057 1106 42-43 0.037567522593569436 1106 44-45 -0.13809900633957994 1106 46-47 -0.06882126611276362 1106 48-49 0.13007606052249798 1106 50-51 -0.07935807140185602 1106 52-53 -0.03810340831106629 1106 54-55 0.08546555548190327 1106 56-57 0.2040003820252636 1106 58-59 0.0215065373308434 1106 60-61 0.06822509980013791 1106 62-63 0.016740960660406756 1106 64-65 0.09546364485169079 1106 66-67 0.037204707708106355 1106 68-69 0.18363347811138198 1106 70-71 0.011346404409788846 1106 72-73 -0.06863732257001942 1106 74-75 0.08271368216822594 1106 76-77 0.03330607264776475 1106 78-79 0.3271356298026049 1106 80-81 0.17806224345457267 1106 82-83 0.2478691032239766 1106 84-85 0.39799901362506773 1106 86-87 0.4965656333977364 1106 88-89 0.42349627040957927 1106 90-91 0.6628248461383883 1106 92-93 0.7253827547287912 1106 94-95 0.8151654214693629 1106 96-97 0.8322824866479444 1106 98-99 0.9891565957090123 1106 100-101 1.6003705025078716 1107 1 0.1200977397082319 1107 2 0.22920192498516556 1107 3 0.21537835239051617 1107 4 0.27012268310630816 1107 5 0.27177688879200446 1107 6 0.1725371443219288 1107 7 0.2760468417170756 1107 8 0.2119597242918374 1107 9 0.3302213069599844 1107 10-11 0.2814986425471986 1107 12-13 0.22000315954810645 1107 14-15 -0.029052956770136973 1107 16-17 0.22115798251490304 1107 18-19 0.30818150633530905 1107 20-21 0.2501960596992774 1107 22-23 0.06415904994253196 1107 24-25 0.10369914952975279 1107 26-27 0.11330392013981339 1107 28-29 0.1453711228568082 1107 30-31 0.053448051064165725 1107 32-33 0.14379435733849988 1107 34-35 0.19100129466094273 1107 36-37 0.14151718308162486 1107 38-39 0.2302171860992459 1107 40-41 0.23040161541115367 1107 42-43 0.20745855836138816 1107 44-45 0.13386222095339662 1107 46-47 0.11693678836567756 1107 48-49 0.1456151554454692 1107 50-51 0.12101164839914702 1107 52-53 0.275538294904095 1107 54-55 0.26993750796333416 1107 56-57 0.39325540030871764 1107 58-59 0.40592684319664585 1107 60-61 0.4968684417136231 1107 62-63 0.45540015559922153 1107 64-65 0.43358575181266445 1107 66-67 0.3532123967015721 1107 68-69 0.3629072585699973 1107 70-71 0.4974694835423499 1107 72-73 0.5228244147447967 1107 74-75 0.6351243178726982 1107 76-77 0.6403493453308471 1107 78-79 0.5482148139149601 1107 80-81 0.48720452297600403 1107 82-83 0.36151973642436985 1107 84-85 0.3758392092377747 1107 86-87 0.47332069771204033 1107 88-89 0.5636857149829062 1107 90-91 0.5309609096536008 1107 92-93 0.7611940208718124 1107 94-95 0.6001461069239831 1107 96-97 0.6978913643421301 1107 98-99 0.8253334388763776 1107 100-101 1.320066367464399 1108 1 0.19492094245021718 1108 2 0.16686181162982194 1108 3 0.3113645714731845 1108 4 0.38954987182283674 1108 5 0.33018269618258245 1108 6 0.35762918144441613 1108 7 0.4326876346522823 1108 8 0.3659841544583955 1108 9 -0.07793365144878095 1108 10-11 0.159019556489433 1108 12-13 0.15424927158609592 1108 14-15 0.43306657908036783 1108 16-17 0.543333762177987 1108 18-19 0.41730288411151406 1108 20-21 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-4.4744740128185185 2316 88-89 -5.794635019576031 2316 90-91 -5.021307877638769 2316 92-93 -4.504538320686002 2316 94-95 -3.894484295430477 2316 96-97 -5.300971722256808 2316 98-99 -7.06160163207992 2316 100-101 -7.761027911684122 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5359.0 3 2028.0 4 2007.0 5 2236.0 6 2398.0 7 2936.0 8 3582.0 9 4557.0 10 5776.0 11 7159.0 12 8894.0 13 10785.0 14 12504.0 15 15015.0 16 18068.0 17 22252.0 18 17885.0 19 14036.0 20 13960.0 21 15072.0 22 16787.0 23 18843.0 24 21952.0 25 25119.0 26 29533.0 27 34651.0 28 41385.0 29 50011.0 30 60766.0 31 74341.0 32 93191.0 33 119438.0 34 158899.0 35 216960.0 36 302099.0 37 390595.0 38 305944.0 39 43226.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4922888251133 18.37924825126098 14.300936179078919 22.8275267445468 2 15.844006969357208 19.667288550113376 40.16605886503607 24.322645615493347 3 19.36783818907957 24.345339588677913 29.455722918799548 26.83109930344297 4 12.812367708527894 16.31758082024252 37.034625145049674 33.835426326179906 5 14.025192791255684 37.24257583548967 33.58043252100541 15.15179885224924 6 32.222039533779096 35.487230077825274 17.59001973035186 14.700710658043773 7 29.231736533239268 32.78479456755622 20.04554757386516 17.937921325339357 8 27.127430673887005 33.833271965946125 19.597697946370083 19.44159941379679 9 27.044092974292806 16.508639123330934 20.187805439705244 36.259462462671024 10-11 25.268401859944937 26.365331708150553 26.864033785283993 21.502232646620513 12-13 26.32250137597603 23.689785347739402 27.51732816286075 22.470385113423816 14-15 23.096084588366654 25.040075878671775 26.00829175011288 25.85554778284869 16-17 22.51407476574035 28.451492537313435 26.451468782434546 22.582963914511673 18-19 22.370668409832074 26.48375763605575 29.171548763179377 21.9740251909328 20-21 23.142610616367435 25.72408349504499 29.29413803519823 21.839167853389345 22-23 22.787193543666167 25.799170629233643 29.217385507176232 22.196250319923962 24-25 22.47900128643236 26.074116222793464 29.31540517596084 22.131477314813335 26-27 22.3222226662176 26.388053226165535 29.035227227318654 22.254496880298213 28-29 22.068796735175443 26.44218410480094 29.230964512200856 22.25805464782276 30-31 22.26744557674045 26.087369420702757 29.256063262473518 22.38912174008328 32-33 21.937608130549787 26.289381893928983 29.19220058254085 22.580809392980377 34-35 22.743359134473458 26.278259166185507 28.79540570300858 22.182975996332452 36-37 22.78361318159508 26.042165580375997 28.68777609095164 22.486445147077287 38-39 22.35734074868068 26.15957836627204 28.86241311566732 22.620667769379956 40-41 22.63939579271159 26.078800534477715 28.951629675338804 22.330173997471892 42-43 22.519783903726896 26.14052649781815 28.671747569601784 22.667942028853165 44-45 22.451188354238045 26.423623539896706 28.534541167790433 22.59064693807482 46-47 22.74902711255315 26.59695191229711 27.99317980046647 22.660841174683263 48-49 22.86412314952462 26.791007228405185 27.64191543606021 22.702954186009986 50-51 22.750426037299523 26.860222098694553 27.59189907053671 22.797452793469215 52-53 23.029283919491878 26.77643580327207 27.535454067512884 22.658826209723166 54-55 22.66876474282397 26.741567955044616 27.629133612189055 22.960533689942363 56-57 22.712876933364047 27.132376324170743 27.384842832236362 22.76990391022885 58-59 22.958692379656988 26.650037873651307 27.474742908906446 22.916526837785256 60-61 22.751174178371922 26.71298316073088 27.87066721731426 22.665175443582935 62-63 22.657867468237484 26.76237940881641 27.908599193817736 22.671153929128373 64-65 22.46587544746635 26.799310671825594 27.7707757818348 22.964038098873253 66-67 22.447918771157624 27.05968456205928 27.4943368505013 22.998059816281792 68-69 22.58142577549363 26.781374683557598 27.792392323973097 22.84480721697567 70-71 22.498491585409365 26.73401474331155 27.709627394772244 23.057866276506832 72-73 22.516640971496628 26.840873221735922 27.643147061617263 22.99933874515019 74-75 22.46382519649982 27.136051509495353 27.397937108641422 23.002186185363406 76-77 22.52897557598362 27.034889429503462 27.425515678253852 23.010619316259067 78-79 22.66054494943487 26.98909192371265 27.370633704135926 22.979729422716556 80-81 22.54398263731211 27.329132845064812 27.273744299899978 22.8531402177231 82-83 22.600433166579194 27.290613133663644 27.206760448654098 22.902193251103064 84-85 22.582990668372457 27.158081551064612 27.12628059899935 23.132647181563577 86-87 22.620049634068028 27.039403861558803 27.19945558216616 23.14109092220701 88-89 22.584723388702695 27.097011022355865 27.03893494314868 23.279330645792758 90-91 22.54668045884958 27.05502117568847 27.288842965678512 23.10945539978344 92-93 22.53206351699613 26.774908813951235 27.43677249788587 23.256255171166764 94-95 22.355800184659053 26.72277671688626 27.48189254523553 23.43953055321916 96-97 22.49023408515467 26.594064424216274 27.397437917091892 23.51826357353717 98-99 22.149659596776992 26.758803263925294 27.273312643052467 23.818224496245243 100-101 22.126228597510007 26.74760696478677 27.441651678920582 23.684512758782645 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 178.0 1 156.0 2 187.5 3 260.5 4 239.0 5 229.0 6 390.5 7 777.0 8 1291.5 9 1613.5 10 1581.5 11 1474.5 12 1572.0 13 1750.5 14 1919.5 15 2121.5 16 2301.0 17 2489.0 18 2749.5 19 3015.5 20 3333.5 21 3888.0 22 4512.0 23 5368.5 24 6627.0 25 8264.0 26 10466.0 27 13272.0 28 16404.5 29 19921.5 30 24075.5 31 28846.5 32 34606.0 33 38584.0 34 41767.0 35 45601.5 36 50581.5 37 55951.5 38 58710.5 39 60118.0 40 62657.5 41 67159.0 42 70944.0 43 74224.0 44 76958.0 45 80402.5 46 81550.5 47 80768.0 48 83607.5 49 83732.0 50 79679.0 51 76350.5 52 74221.5 53 74233.0 54 74205.0 55 69947.5 56 63856.0 57 58058.0 58 53298.5 59 47300.0 60 40582.0 61 34433.0 62 28893.5 63 23296.5 64 17795.0 65 14046.0 66 10376.5 67 7992.0 68 5778.0 69 3641.0 70 2706.0 71 2157.0 72 1825.5 73 1686.5 74 1363.0 75 1076.5 76 821.0 77 510.0 78 320.0 79 221.0 80 152.0 81 98.0 82 68.0 83 49.0 84 33.0 85 17.5 86 11.5 87 11.5 88 10.5 89 6.5 90 4.0 91 3.5 92 3.0 93 5.5 94 5.0 95 3.5 96 2.0 97 1.0 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07711392132279823 2 0.031092113925888457 3 0.016071107344952623 4 0.02278262092366863 5 0.02702868053469305 6 0.034059789998109824 7 0.08149695059869441 8 0.05770988546596623 9 0.07387230592083335 10-11 0.06318867206083645 12-13 0.04159312156605636 14-15 0.04481190869054261 16-17 0.05670544125690669 18-19 0.05227675542605327 20-21 0.0187876723649091 22-23 0.013354542324996142 24-25 0.022326055374096116 26-27 0.02527090316883885 28-29 0.006072321809314485 30-31 0.001187070428888546 32-33 0.009359593766236613 34-35 0.01125434079696256 36-37 0.0041547465011099105 38-39 0.009747674483373252 40-41 0.0041319182236312845 42-43 0.0017577773658541932 44-45 4.565655495725176E-4 46-47 3.4242416217938827E-4 48-49 8.218179892305318E-4 50-51 0.0010729290414954165 52-53 1.141413873931294E-4 54-55 2.5111105226488474E-4 56-57 0.0034014133443152567 58-59 0.004063433391195408 60-61 0.005364645207477083 62-63 0.0030361609046572423 64-65 7.989897117519061E-4 66-67 4.3373727209389175E-4 68-69 0.004405857553374796 70-71 0.0023969691352557177 72-73 0.022371711929053364 74-75 0.017281006051319794 76-77 0.026343832210334272 78-79 0.02129878288755795 80-81 0.012897976775423623 82-83 0.005273332097562579 84-85 0.003652524396580141 86-87 0.0016664642559396895 88-89 0.0016208077009824378 90-91 0.006232119751664867 92-93 0.010866260079825921 94-95 0.012966461607859503 96-97 0.011459795294270193 98-99 0.0232848430281984 100-101 0.01924423791448162 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2190266.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.202632021934036 #Duplication Level Percentage of deduplicated Percentage of total 1 77.25020236044887 42.644144945238 2 13.025684723782794 14.38104161281418 3 4.259998037098527 7.05489312168334 4 1.8536380355907545 4.093027935223083 5 0.942739191504315 2.602084234063415 6 0.5785841818041931 1.9163621809109195 7 0.3573532860675311 1.3808789366830392 8 0.2626166664268369 1.1597704959670143 9 0.19596249398032115 0.9735880900766519 >10 1.1035729876632048 11.349834400019864 >50 0.10312768113644208 3.9168303401498004 >100 0.06234974985197427 6.134964611977453 >500 0.003253071622652667 1.1798742659924226 >1k 7.507088359967436E-4 0.6162292109653514 >5k 1.6682418577705413E-4 0.5964756182354601 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7219 0.32959467023640054 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5735 0.26184034267983886 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3248 0.14829249050115373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.565655495725177E-5 0.0 14-15 0.0 0.0 0.0 4.565655495725177E-5 0.0 16-17 0.0 0.0 0.0 4.565655495725177E-5 0.0 18-19 2.2828277478625884E-5 0.0 0.0 4.565655495725177E-5 0.0 20-21 4.565655495725177E-5 0.0 0.0 4.565655495725177E-5 0.0 22-23 4.565655495725177E-5 0.0 0.0 1.1414138739312942E-4 0.0 24-25 4.565655495725177E-5 0.0 0.0 2.0545449730763294E-4 0.0 26-27 4.565655495725177E-5 0.0 0.0 3.6525243965801414E-4 2.2828277478625884E-5 28-29 6.848483243587765E-5 0.0 0.0 8.903028216664095E-4 4.565655495725177E-5 30-31 9.131310991450354E-5 0.0 0.0 0.0024426256902129696 4.565655495725177E-5 32-33 9.131310991450354E-5 0.0 0.0 0.004679796883118306 4.565655495725177E-5 34-35 9.131310991450354E-5 0.0 0.0 0.007601816400382419 4.565655495725177E-5 36-37 9.131310991450354E-5 0.0 0.0 0.011094542854612179 4.565655495725177E-5 38-39 9.131310991450354E-5 0.0 0.0 0.014062218926833545 4.565655495725177E-5 40-41 9.131310991450354E-5 0.0 0.0 0.01648201633956789 4.565655495725177E-5 42-43 9.131310991450354E-5 0.0 0.0 0.02013454073614803 4.565655495725177E-5 44-45 9.131310991450354E-5 0.0 0.0 0.02401534790751443 4.565655495725177E-5 46-47 1.1414138739312942E-4 0.0 0.0 0.02999635660691441 4.565655495725177E-5 48-49 1.369696648717553E-4 0.0 0.0 0.037050294347809806 4.565655495725177E-5 50-51 1.369696648717553E-4 0.0 0.0 0.04373897964904719 4.565655495725177E-5 52-53 1.369696648717553E-4 0.0 0.0 0.05677392608934257 4.565655495725177E-5 54-55 1.8262621982900707E-4 0.0 0.0 0.07688563854801198 4.565655495725177E-5 56-57 2.2828277478625884E-4 0.0 0.0 0.1166753261932569 4.565655495725177E-5 58-59 2.2828277478625884E-4 0.0 0.0 0.1646375371758499 4.565655495725177E-5 60-61 2.2828277478625884E-4 0.0 0.0 0.21084197079258865 4.565655495725177E-5 62-63 2.2828277478625884E-4 0.0 0.0 0.2645112511448381 4.565655495725177E-5 64-65 2.2828277478625884E-4 0.0 0.0 0.3309415386076394 4.565655495725177E-5 66-67 2.2828277478625884E-4 0.0 0.0 0.4336459589839773 4.565655495725177E-5 68-69 2.2828277478625884E-4 0.0 0.0 0.5601602727705219 4.565655495725177E-5 70-71 2.2828277478625884E-4 0.0 0.0 0.6675444900299781 4.565655495725177E-5 72-73 2.5111105226488474E-4 0.0 0.0 0.7792889082878518 4.565655495725177E-5 74-75 2.739393297435106E-4 0.0 0.0 0.901739788683201 4.565655495725177E-5 76-77 2.739393297435106E-4 0.0 0.0 1.0452611691913218 4.565655495725177E-5 78-79 2.739393297435106E-4 0.0 0.0 1.1676435647542354 4.565655495725177E-5 80-81 3.6525243965801414E-4 0.0 0.0 1.2817164673149288 4.565655495725177E-5 82-83 4.109089946152659E-4 0.0 0.0 1.3955839153783147 4.565655495725177E-5 84-85 5.250503820083953E-4 2.2828277478625884E-5 0.0 1.5983218476659913 4.565655495725177E-5 86-87 5.93535214444273E-4 4.565655495725177E-5 0.0 1.876530065298005 4.565655495725177E-5 88-89 5.93535214444273E-4 4.565655495725177E-5 0.0 2.2071291797434647 4.565655495725177E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5215 0.0 46.73954 1 GTATCAA 9155 0.0 40.4816 1 ATCAACG 11495 0.0 31.830454 3 TCAACGC 11630 0.0 31.542685 4 CAACGCA 11835 0.0 30.997736 5 TATCAAC 11820 0.0 30.991203 2 AACGCAG 12330 0.0 29.983858 6 GTACATG 11180 0.0 26.476933 1 ACGCAGA 14020 0.0 26.132284 7 CGCAGAG 14210 0.0 25.722456 8 TACATGG 11415 0.0 25.4312 2 ACATGGG 11530 0.0 23.944616 3 GCAGAGT 15145 0.0 23.696686 9 GAGTACT 7610 0.0 23.357021 12-13 GTCCGTA 125 2.2523172E-4 22.80671 1 CATGGGG 5110 0.0 21.573875 4 GTACTTT 8330 0.0 21.163137 14-15 GTATAGG 685 0.0 20.115408 1 AGTACTT 8095 0.0 20.10825 12-13 GTGGTAT 3005 0.0 19.922667 1 >>END_MODULE