FastQCFastQC Report
Thu 26 May 2016
SRR936692_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936692_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2190266
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92620.4228710120140659No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82720.37767102260638663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46670.213079141985494No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24710.11281734729936913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58500.053.8406681
GTATCAA104600.040.421381
ATCAACG136750.030.803523
TCAACGC138100.030.4652274
TATCAAC138950.030.3185772
CAACGCA141050.029.7943955
AACGCAG145100.028.8966676
ACGCAGA164850.025.3770667
CGCAGAG167250.025.012918
GTAAGGT8950.024.4061454
GTACATG110350.023.8501011
TAAGGTG11050.023.6355025
GCAGAGT178150.023.2426229
TACATGG112650.023.1886372
GAGTACT102650.022.9922612-13
ACATGGG111400.022.2104113
GTGGTAT29700.022.0737171
GTACTTT108300.021.79275514-15
TGGTATC29250.021.5958082
CATGGGG49850.021.051914
Produced by FastQC (version 0.11.5)