FastQCFastQC Report
Thu 26 May 2016
SRR936687_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936687_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008438
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58330.5784192979637816No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51720.5128723828336497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45090.44712714118270036No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42040.4168823467580555No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35970.35669024768999186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35780.3548061457422271No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25890.25673368119805084No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20850.20675539795208037No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC19660.19495496996344844No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT17280.17135411398618458No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14290.14170429912399174No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC13220.1310938302602639No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA11040.10947623949117348No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG10770.10679883146013935No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC10770.10679883146013935No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT10720.10630301515809598No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA10170.10084903583561905No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10090.10005572975234968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT41100.025.25770812-13
GTACTTT44650.022.87882214-15
GTACATG43000.022.869921
GCTCCGT1050.002193371322.6237726
GTACACG1903.281075E-722.5035821
TACATGG43400.022.0045362
AGTACTT43600.021.79354112-13
ACCGTCC1100.00287281121.596498
ACATGGG42750.021.5600593
TAGACTG4500.021.115525
ATTCCGA1150.00371846220.6564886
ACTTTTT52750.019.68239616-17
AGAGTAC72850.019.59792310-11
TACCTGG7350.019.3927672
AGGTGCG1250.00602164219.0049137
CTCCTAA8200.018.5395371
TACTTTT50650.018.43315714-15
CTACACT4151.8189894E-1218.3170784
CATGGGA21600.017.8162214
GTATAGG3208.250936E-917.8153361
Produced by FastQC (version 0.11.5)