##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936687_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1008438 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.59848795860529 31.0 31.0 34.0 30.0 34.0 2 31.67293080982668 33.0 31.0 34.0 30.0 34.0 3 31.683813977656534 33.0 31.0 34.0 30.0 34.0 4 35.29828407894189 37.0 35.0 37.0 33.0 37.0 5 35.30750427889468 37.0 35.0 37.0 33.0 37.0 6 35.29666573453202 37.0 35.0 37.0 33.0 37.0 7 35.229626412332735 37.0 35.0 37.0 32.0 37.0 8 35.18712008075856 37.0 35.0 37.0 32.0 37.0 9 36.716801627864086 39.0 37.0 39.0 32.0 39.0 10-11 36.59449118339452 39.0 37.0 39.0 32.0 39.0 12-13 36.50711248485281 39.0 37.0 39.0 32.0 39.0 14-15 37.63074527139993 40.0 37.0 41.0 32.0 41.0 16-17 37.54486195482518 40.0 37.0 41.0 32.0 41.0 18-19 37.53910899827258 40.0 37.0 41.0 32.0 41.0 20-21 37.41091023939994 40.0 37.0 41.0 32.0 41.0 22-23 37.52232859134622 40.0 37.0 41.0 32.0 41.0 24-25 37.557724917149095 40.0 37.0 41.0 32.0 41.0 26-27 37.47524339622267 40.0 37.0 41.0 32.0 41.0 28-29 37.34212316473596 40.0 37.0 41.0 31.5 41.0 30-31 37.11901822422399 39.5 36.5 41.0 31.0 41.0 32-33 36.90912480489628 39.0 36.0 41.0 30.5 41.0 34-35 36.69688171211319 39.0 35.5 41.0 30.0 41.0 36-37 36.48204599588671 39.0 35.0 41.0 30.0 41.0 38-39 36.278044857492475 39.0 35.0 40.0 30.0 41.0 40-41 36.065921752254475 39.0 35.0 40.0 29.5 41.0 42-43 35.80604657896668 38.5 35.0 40.0 28.5 41.0 44-45 35.48574329805105 38.0 35.0 40.0 27.0 41.0 46-47 35.23469315912332 38.0 35.0 40.0 26.0 41.0 48-49 35.022032589013904 38.5 34.5 40.0 25.0 41.0 50-51 34.030871506230426 37.5 33.0 39.5 23.5 40.5 52-53 34.12398084959115 38.0 33.5 39.5 24.0 40.5 54-55 34.55050831087286 38.0 34.0 40.0 24.0 41.0 56-57 34.4022860106422 38.0 34.0 40.0 23.0 41.0 58-59 34.157318050291636 38.0 34.0 40.0 23.0 41.0 60-61 33.84740410416902 37.5 33.5 40.0 22.0 41.0 62-63 33.49215717773428 37.0 33.0 40.0 21.0 41.0 64-65 33.14411842869864 36.5 32.5 39.5 20.0 41.0 66-67 32.64946927823029 36.0 32.0 39.0 18.0 41.0 68-69 32.25432996376574 35.5 31.5 39.0 17.0 40.5 70-71 31.828381120108524 35.0 31.0 38.5 13.0 40.0 72-73 31.44158788145627 35.0 31.0 37.5 10.0 40.0 74-75 31.027169245903067 35.0 31.0 37.0 7.5 39.0 76-77 30.559307066968916 35.0 30.0 36.5 6.5 39.0 78-79 30.062535326911522 34.0 30.0 36.0 2.0 38.5 80-81 29.578582421527152 34.0 29.0 35.5 2.0 37.0 82-83 29.168129324757693 34.0 29.0 35.0 2.0 37.0 84-85 28.80536483155137 34.0 29.0 35.0 2.0 36.5 86-87 28.47244500901394 34.0 29.0 35.0 2.0 36.0 88-89 28.056167062328075 34.0 27.5 35.0 2.0 36.0 90-91 27.637026768130514 34.0 26.5 35.0 2.0 35.0 92-93 27.181873848466637 33.0 25.5 35.0 2.0 35.0 94-95 26.69565109605152 33.0 25.0 35.0 2.0 35.0 96-97 26.134357788976615 33.0 24.5 35.0 2.0 35.0 98-99 25.387570182797553 33.0 20.5 35.0 2.0 35.0 100-101 22.51789797687116 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5266907837948764 1101 2 0.4655197439062846 1101 3 0.4215335230434505 1101 4 0.4446263564348101 1101 5 0.4661103602615597 1101 6 0.558896904810176 1101 7 0.5532387836366297 1101 8 0.4059398543182766 1101 9 0.5332818511539159 1101 10-11 0.5881882157715239 1101 12-13 0.5549873461665271 1101 14-15 0.680040542704262 1101 16-17 0.8694475774467492 1101 18-19 0.9099894311579817 1101 20-21 0.7883242150231027 1101 22-23 0.7664886393362522 1101 24-25 0.7705686785559536 1101 26-27 0.6941908999194126 1101 28-29 0.6691531413850171 1101 30-31 0.6637417607816545 1101 32-33 0.6745690069481114 1101 34-35 0.7526144015781142 1101 36-37 0.6855947576945951 1101 38-39 0.6921928320425152 1101 40-41 0.5106446828342186 1101 42-43 0.80057708000394 1101 44-45 0.5383730918210645 1101 46-47 0.6715196912819152 1101 48-49 0.8662265702231977 1101 50-51 0.6809516354452896 1101 52-53 0.9194672290201567 1101 54-55 0.7445207443337765 1101 56-57 0.798244753386733 1101 58-59 0.7097605646524912 1101 60-61 0.8086661675481608 1101 62-63 0.9103600525289224 1101 64-65 0.7751675327559582 1101 66-67 0.9129938773762518 1101 68-69 0.9427979079218076 1101 70-71 0.8341689252700597 1101 72-73 0.8047013456042365 1101 74-75 0.7665294969850258 1101 76-77 0.4700134029260177 1101 78-79 0.4596870168833185 1101 80-81 0.5739330927601642 1101 82-83 0.47644028790790927 1101 84-85 0.5089718473772287 1101 86-87 0.6092255355265799 1101 88-89 0.8225613429972434 1101 90-91 0.8444320754881147 1101 92-93 0.7649389385435406 1101 94-95 0.6884358707464706 1101 96-97 0.7238577196906135 1101 98-99 0.6474746566286811 1101 100-101 0.9762778717662002 1102 1 0.4773153825031855 1102 2 0.5744043418592284 1102 3 0.43035249800285413 1102 4 0.4444162684339048 1102 5 0.4785763684951192 1102 6 0.6047031706257897 1102 7 0.6117589143160913 1102 8 0.5418431855113823 1102 9 0.7926319474639243 1102 10-11 0.7544834909159874 1102 12-13 0.6952576751312876 1102 14-15 0.8917514144042258 1102 16-17 0.7830848852988481 1102 18-19 0.6687281477799516 1102 20-21 0.5549376908700694 1102 22-23 0.6394337898442402 1102 24-25 0.7726140858906163 1102 26-27 0.5391978871158543 1102 28-29 0.40167098706239557 1102 30-31 0.7663874532694805 1102 32-33 0.7859475147095552 1102 34-35 0.8526977286166755 1102 36-37 0.7145774596623369 1102 38-39 0.5602705504396184 1102 40-41 0.6091145812366108 1102 42-43 0.6702280978685096 1102 44-45 0.6909677966592298 1102 46-47 0.958096248293657 1102 48-49 1.1040450069796321 1102 50-51 1.0303504060764013 1102 52-53 1.4101808672722314 1102 54-55 1.2734137458067565 1102 56-57 1.2025827165245389 1102 58-59 1.1896133614194966 1102 60-61 1.1064682693723853 1102 62-63 1.074336097775749 1102 64-65 1.2035865379302635 1102 66-67 1.2916834003584654 1102 68-69 1.2782057619082892 1102 70-71 1.1287276460465812 1102 72-73 0.7188839380429641 1102 74-75 0.9123447328501193 1102 76-77 1.1246889231888844 1102 78-79 1.0792589566801247 1102 80-81 1.306635060505755 1102 82-83 1.4420696549056764 1102 84-85 0.9086760717984248 1102 86-87 1.0240655381703867 1102 88-89 1.037530939250594 1102 90-91 1.0383739873361328 1102 92-93 1.219018186570132 1102 94-95 0.7618958548836439 1102 96-97 0.9798511290647873 1102 98-99 1.2290088655647367 1102 100-101 1.5686717419176546 1103 1 0.4287357385559112 1103 2 0.32165018213251173 1103 3 0.4778702388160596 1103 4 0.6722963170196081 1103 5 0.5884366724709551 1103 6 0.5652581968460737 1103 7 0.6197583421491046 1103 8 0.6055567674464228 1103 9 0.4131464371079474 1103 10-11 0.6817503995414 1103 12-13 0.37476355075284573 1103 14-15 0.7025880995208524 1103 16-17 0.8933937100048226 1103 18-19 0.8991956760913098 1103 20-21 0.703763233277094 1103 22-23 0.6039303435079333 1103 24-25 0.6323740788112673 1103 26-27 0.5127109196736086 1103 28-29 0.5694631983180827 1103 30-31 0.5275298598135194 1103 32-33 0.48926893193809207 1103 34-35 0.7742132680416489 1103 36-37 0.8222457700968633 1103 38-39 0.8023250663160368 1103 40-41 0.585867266906007 1103 42-43 0.5904383813578491 1103 44-45 0.8297776893294255 1103 46-47 0.9846556457727331 1103 48-49 0.9835814304995765 1103 50-51 0.9350885427030917 1103 52-53 0.8064886324995086 1103 54-55 0.993663486998166 1103 56-57 0.9843613194188379 1103 58-59 0.9039753588641375 1103 60-61 0.9703182864646678 1103 62-63 1.1586819363519183 1103 64-65 1.2005675085291259 1103 66-67 1.026397025933349 1103 68-69 0.952745179607156 1103 70-71 0.8323096901858698 1103 72-73 0.8887841967303274 1103 74-75 0.950301708802133 1103 76-77 0.7682064651983076 1103 78-79 0.9157534519973289 1103 80-81 0.8628998647203971 1103 82-83 0.7032608532318498 1103 84-85 0.5800616008225639 1103 86-87 0.6501225924316962 1103 88-89 0.5127874446195086 1103 90-91 0.6715328218610743 1103 92-93 0.637881695650858 1103 94-95 0.5205380614116812 1103 96-97 0.5440532349302281 1103 98-99 0.6558454367328537 1103 100-101 0.8208597957498363 1104 1 0.3948721616800732 1104 2 0.4235920791892269 1104 3 0.4242365249438578 1104 4 0.4129986972529096 1104 5 0.4249091849631199 1104 6 0.5176357653057551 1104 7 0.42850055045889945 1104 8 0.36899613764453676 1104 9 0.5408869268933216 1104 10-11 0.7037530785918662 1104 12-13 0.7220810656078456 1104 14-15 0.5114863488999433 1104 16-17 0.38266507223447377 1104 18-19 0.41937382939411094 1104 20-21 0.2894635349367576 1104 22-23 0.3725128510375768 1104 24-25 0.40947518513356584 1104 26-27 0.35482578248183216 1104 28-29 0.30902887709969207 1104 30-31 0.37354959856464376 1104 32-33 0.3714505222897131 1104 34-35 0.363736723945685 1104 36-37 0.2677687830456108 1104 38-39 0.21065315880131408 1104 40-41 0.04460402710256517 1104 42-43 0.1891658916056329 1104 44-45 0.28041630295042097 1104 46-47 0.2414598654548712 1104 48-49 0.28066053118188705 1104 50-51 0.06027078801268004 1104 52-53 0.18861426125322822 1104 54-55 0.10912617985841422 1104 56-57 -0.1254421458180559 1104 58-59 0.05818834929797134 1104 60-61 0.043556917377493676 1104 62-63 0.0696417044044253 1104 64-65 -0.005746321743195892 1104 66-67 0.09634250926982446 1104 68-69 0.12866445988770892 1104 70-71 0.0027231190743748357 1104 72-73 0.05017981384166248 1104 74-75 0.25320821799464 1104 76-77 -0.0038785277369122184 1104 78-79 0.14961845012009434 1104 80-81 0.3182111237201468 1104 82-83 0.15953234218935464 1104 84-85 -0.006599819074182278 1104 86-87 0.2495746425211749 1104 88-89 0.2481065560085547 1104 90-91 0.08635305188989051 1104 92-93 -0.2830214712426198 1104 94-95 -0.16868557060853817 1104 96-97 0.058353051707808135 1104 98-99 0.09861478375279376 1104 100-101 -0.3483233662640224 1105 1 0.2671106987488052 1105 2 0.2506124677428083 1105 3 0.3966528237757956 1105 4 0.33043015988390323 1105 5 0.18030419442172985 1105 6 0.3567217335368511 1105 7 0.5244997096937993 1105 8 0.6042938585705784 1105 9 0.5631359735257604 1105 10-11 0.5087646391119449 1105 12-13 0.40042025426951966 1105 14-15 0.5077416412159508 1105 16-17 0.5616717683279191 1105 18-19 0.435072114833865 1105 20-21 0.37913923982821274 1105 22-23 0.24653441065682813 1105 24-25 0.36416335136483013 1105 26-27 0.21796363793028917 1105 28-29 0.08845505492151062 1105 30-31 0.22268931570724249 1105 32-33 -0.21672351608520302 1105 34-35 0.06522277625328599 1105 36-37 0.1295848356534819 1105 38-39 0.06594637562862715 1105 40-41 -0.05787232637910478 1105 42-43 0.02857495385227793 1105 44-45 0.03652806465357372 1105 46-47 0.36483944086850073 1105 48-49 0.30682514097680524 1105 50-51 0.23229613107486813 1105 52-53 0.25915775583330003 1105 54-55 -0.028375546712759103 1105 56-57 -0.04684472216252544 1105 58-59 0.08510724549350357 1105 60-61 8.035370455417024E-5 1105 62-63 0.0668362514422256 1105 64-65 0.05317839975336369 1105 66-67 0.22607856908425816 1105 68-69 0.3201351982596279 1105 70-71 0.2125462581309172 1105 72-73 0.10718008408758806 1105 74-75 0.1773468841808672 1105 76-77 0.015366285042716754 1105 78-79 0.012634394629596812 1105 80-81 0.008422816360447172 1105 82-83 -0.01752528024706379 1105 84-85 0.10797934796782727 1105 86-87 -0.05720282497424378 1105 88-89 0.013216906427434338 1105 90-91 -0.19136877497255256 1105 92-93 -0.37095138746638057 1105 94-95 -0.37431749566033545 1105 96-97 -0.2899534071697687 1105 98-99 -0.10395075088372252 1105 100-101 -0.3411819675912291 1106 1 0.14073582652503447 1106 2 0.06544194455723229 1106 3 0.1035982749420441 1106 4 0.06580419575417551 1106 5 0.06232816988869416 1106 6 0.42716500089118625 1106 7 0.3230110004660318 1106 8 0.26478637785317005 1106 9 0.3652435843426005 1106 10-11 0.3418331271706734 1106 12-13 0.3282141395961702 1106 14-15 0.0776719450910619 1106 16-17 0.12232877046526625 1106 18-19 0.1733771050764048 1106 20-21 -0.03702933186587387 1106 22-23 0.06836974194575163 1106 24-25 -0.025764164665275757 1106 26-27 -0.160831083749855 1106 28-29 9.743147508345373E-6 1106 30-31 0.12053338224381349 1106 32-33 0.12239645417778178 1106 34-35 0.052298326852678656 1106 36-37 0.029620148356762854 1106 38-39 -0.13849187360471404 1106 40-41 -0.17649606023283582 1106 42-43 -0.07193429250029482 1106 44-45 -0.05161244273421772 1106 46-47 -0.1327602245901005 1106 48-49 -0.07767165300145251 1106 50-51 -0.09460378528978453 1106 52-53 -0.31971223766173296 1106 54-55 -0.1521893186670411 1106 56-57 -0.13880736503958957 1106 58-59 -0.17843796405950485 1106 60-61 -0.07279391448613381 1106 62-63 -0.14653518243938635 1106 64-65 -0.09238799742161774 1106 66-67 -0.021872364843815717 1106 68-69 0.24195008535190965 1106 70-71 -0.1669379905448558 1106 72-73 -0.4941683036087241 1106 74-75 -0.21223865619641913 1106 76-77 -0.2385524957397358 1106 78-79 -0.4677103688682145 1106 80-81 -0.1943176352704441 1106 82-83 -0.2963469510955008 1106 84-85 -0.24802474087150017 1106 86-87 -0.20079356932886228 1106 88-89 -0.29620043396844054 1106 90-91 -0.573174369248175 1106 92-93 -0.5038191506854126 1106 94-95 -0.6328278571509038 1106 96-97 -0.6184962935186213 1106 98-99 -0.4737834801839753 1106 100-101 -0.4875168807033958 1107 1 0.17870862527573905 1107 2 0.3055955644297619 1107 3 0.22425706087293307 1107 4 0.2234479647069776 1107 5 0.3794297296933067 1107 6 0.3863994437013716 1107 7 0.40211070811615457 1107 8 0.5471200925124293 1107 9 0.5356309025318424 1107 10-11 0.5272455947125678 1107 12-13 0.4474520324980631 1107 14-15 0.21417391527500484 1107 16-17 0.354157682233712 1107 18-19 0.45833107358978253 1107 20-21 0.39949533628978173 1107 22-23 0.43211926528834255 1107 24-25 0.4400209127802057 1107 26-27 0.4078847558027192 1107 28-29 0.39604576936820735 1107 30-31 0.4972216573795407 1107 32-33 0.26699070523550716 1107 34-35 0.24035011454492405 1107 36-37 0.24300831998974814 1107 38-39 0.36229075245982045 1107 40-41 0.28638730850934024 1107 42-43 0.42358058828995837 1107 44-45 0.3196337667045057 1107 46-47 0.2540123054166159 1107 48-49 0.39960404564211416 1107 50-51 0.3081717226908225 1107 52-53 0.4228854998044511 1107 54-55 0.6578268422888129 1107 56-57 0.5233333260682613 1107 58-59 0.5594322398590705 1107 60-61 0.5449304776621844 1107 62-63 0.7320424718614262 1107 64-65 0.6383305295708084 1107 66-67 0.622517604468527 1107 68-69 0.7085958698845971 1107 70-71 0.40520796649242286 1107 72-73 0.40183412961384235 1107 74-75 0.467935119474987 1107 76-77 0.4907460461425579 1107 78-79 0.35699319562126064 1107 80-81 0.2667099473047827 1107 82-83 0.35549703212615924 1107 84-85 0.044508534355024665 1107 86-87 0.23318257056327596 1107 88-89 0.03294194586849741 1107 90-91 0.03204344048834784 1107 92-93 0.07237067699158573 1107 94-95 0.3208909785339422 1107 96-97 0.25956203118948196 1107 98-99 0.3705275001868209 1107 100-101 0.09613604007160603 1108 1 0.27902331491189614 1108 2 0.12616542838116018 1108 3 0.23862820449880218 1108 4 0.3019321303991589 1108 5 0.28180178601419925 1108 6 0.21197011576815328 1108 7 0.3312646588496406 1108 8 0.24974123851511365 1108 9 0.3553337062168538 1108 10-11 0.333447167210025 1108 12-13 0.1315796957135973 1108 14-15 0.37559353631642267 1108 16-17 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-2.724691811708503 2316 86-87 -2.6279594390770633 2316 88-89 -2.742388668394266 2316 90-91 -2.8149099645295728 2316 92-93 -3.4190518912941634 2316 94-95 -3.3391634028775066 2316 96-97 -3.7323796323873317 2316 98-99 -4.739137559756326 2316 100-101 -5.781085564180032 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2207.0 3 880.0 4 854.0 5 989.0 6 1037.0 7 1262.0 8 1523.0 9 1920.0 10 2456.0 11 2979.0 12 3895.0 13 4815.0 14 5777.0 15 7582.0 16 9257.0 17 12256.0 18 9576.0 19 7029.0 20 6980.0 21 7171.0 22 7674.0 23 8765.0 24 10017.0 25 11373.0 26 13226.0 27 15261.0 28 18345.0 29 21808.0 30 26374.0 31 32171.0 32 40857.0 33 53394.0 34 71275.0 35 98648.0 36 139818.0 37 183586.0 38 143204.0 39 22187.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.894283608926216 17.736293663363785 14.656093209337199 22.713329518372806 2 15.51767056921696 19.524652514343234 39.64366294263201 25.314013973807786 3 20.089000583155148 23.560062996624048 30.089317946500476 26.261618473720333 4 12.346015750530638 15.928963916605504 37.88835769970839 33.83666263315546 5 13.558129937741306 36.678464999821436 34.413105673120036 15.350299389317218 6 31.56220080265501 35.54362762363517 17.315818455112336 15.578353118597487 7 28.066121701774026 32.8269119967314 20.520576011297294 18.586390290197276 8 26.456038860106197 32.143803025208086 20.06995136535106 21.33020674933466 9 25.875097095228224 16.877953158186603 21.12871528752588 36.118234459059295 10-11 25.117190871562773 25.342098135759862 27.11070060547377 22.4300103872036 12-13 26.941430357691097 23.253418219891962 27.38087503056521 22.424276391851723 14-15 23.2252477543273 24.45483136437605 27.173644028328386 25.146276852968263 16-17 21.75860736659741 27.908713828774694 27.6611204493185 22.671558355309397 18-19 21.537428440923435 26.48857509716858 30.21403621708828 21.759960244819702 20-21 22.531006290556228 25.040382592820432 29.792431488500387 22.636179628122953 22-23 21.746639789945032 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29.193664185572366 22.221015721530385 48-49 22.295305979976835 25.93977581036698 28.910774350675105 22.85414385898107 50-51 21.589948408241792 26.386717539462364 29.67505373624984 22.348280316046004 52-53 22.371204255458405 26.64907442391355 28.397043233878655 22.582678086749393 54-55 21.532382544262365 25.987204361802295 29.094879414274526 23.385533679660806 56-57 22.40522396200034 26.06883968148508 28.336919766369505 23.189016590145076 58-59 22.901222110546783 25.831371850946844 28.347044289421085 22.920361749085288 60-61 22.16925093811235 25.910048313777757 29.663687713703187 22.257013034406707 62-63 21.838529358974487 26.224402066180037 29.377468562549147 22.55960001229633 64-65 21.477930308533903 26.743389851853944 28.951920831430623 22.826759008181526 66-67 21.73766643243348 26.400278897837797 28.29409638141957 23.56795828830915 68-69 21.74989000501488 26.02637597898017 29.073844705434176 23.149889310570778 70-71 22.322125284558368 26.070383457441874 28.393927503282747 23.213563754717008 72-73 21.976817128051277 25.806131683671573 29.318770687923827 22.898280500353323 74-75 21.631412327718795 27.04050533340806 28.468947101380632 22.859135237492517 76-77 21.786999903782345 26.228386985421537 28.51603611038645 23.468577000409667 78-79 21.959123089615375 26.182891903248358 28.790941774289486 23.067043232846785 80-81 22.474729680777685 26.400772127966814 28.147389105850014 22.97710908540549 82-83 22.138386868903336 26.087081616940893 28.962237382035227 22.81229413212055 84-85 21.928449930324476 26.500205803153005 28.311471914068502 23.259872352454018 86-87 21.898209496453113 26.759259993969497 28.41343254566518 22.92909796391221 88-89 21.72988655384043 26.57407476287893 27.725943834852146 23.97009484842849 90-91 21.74009438758429 26.06947382517713 28.359476329944872 23.830955457293708 92-93 22.270426823976333 26.1892328178119 28.70564197172691 22.83469838648486 94-95 21.7775699479373 25.856302194726094 28.741098290346113 23.625029566990495 96-97 21.056878233408447 25.936597422794932 29.512489288094706 23.494035055701907 98-99 21.33867134983356 26.565909124733967 28.21027617786112 23.885143347571354 100-101 21.124339687615056 26.13710680214793 28.511154974808147 24.22739853542887 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 52.0 1 49.5 2 77.0 3 108.0 4 97.5 5 103.5 6 199.0 7 494.0 8 886.5 9 1077.5 10 987.0 11 891.0 12 954.5 13 1044.0 14 1163.0 15 1259.0 16 1314.0 17 1397.5 18 1522.0 19 1676.0 20 1792.0 21 2075.0 22 2554.5 23 3041.5 24 3574.0 25 4333.0 26 5565.0 27 7033.0 28 8392.0 29 10016.5 30 11911.0 31 14231.0 32 17182.5 33 19110.5 34 20245.0 35 21860.0 36 23865.0 37 25612.5 38 26541.0 39 26992.0 40 27438.5 41 28489.5 42 29092.0 43 29856.5 44 31315.5 45 32985.0 46 34584.5 47 40395.0 48 53109.0 49 51971.0 50 39120.5 51 33613.0 52 32043.0 53 31870.0 54 31471.0 55 29541.0 56 27303.5 57 25007.5 58 23456.0 59 21140.5 60 17470.0 61 14832.5 62 12585.5 63 9703.0 64 7001.0 65 5328.0 66 3962.5 67 3046.5 68 2356.0 69 1683.5 70 1243.5 71 923.0 72 744.0 73 654.0 74 532.0 75 423.5 76 302.0 77 182.0 78 124.0 79 92.0 80 69.0 81 43.5 82 25.0 83 15.0 84 9.5 85 7.0 86 5.5 87 7.0 88 4.5 89 1.5 90 0.0 91 0.0 92 0.5 93 2.5 94 2.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012593734071901296 2 0.012197081030266609 3 0.012891223853127312 4 0.021617590769090415 5 0.038277018517747244 6 0.05285401779782198 7 0.0054539793224769395 8 0.03064144746627953 9 0.04035944698632935 10-11 0.04630924261084965 12-13 0.034459232992013394 14-15 0.03297178408588332 16-17 0.03307094734629199 18-19 0.026526172159319662 20-21 0.0573163645162122 22-23 0.012940805483331649 24-25 0.0035202957445078426 26-27 0.0013882856457214027 28-29 0.0 30-31 0.0027765712914428055 32-33 0.001090795864495388 34-35 0.0 36-37 2.479081510216791E-4 38-39 0.0 40-41 0.0011403774946997238 42-43 0.0028757345518514773 44-45 7.933060832693731E-4 46-47 0.0011899591249040595 48-49 5.949795624520298E-4 50-51 0.004016112046551201 52-53 0.003867367155938194 54-55 0.004710254869411902 56-57 7.933060832693731E-4 58-59 0.0059002139943159625 60-61 0.002974897812260149 62-63 6.941428228607014E-4 64-65 7.437244530650373E-4 66-67 0.018196458284991245 68-69 0.04338392642879384 70-71 0.051217824001078896 72-73 0.01492407069150508 74-75 0.030889355617301214 76-77 0.030443120945462192 78-79 0.055382680938243105 80-81 0.08260299592042346 82-83 0.05057326280842253 84-85 0.019138509258873622 86-87 0.022807549893994476 88-89 0.024840396732372243 90-91 0.035748355377326124 92-93 0.02335294782624217 94-95 0.013337458524966334 96-97 0.02122093772745573 98-99 0.05682054821416885 100-101 0.07645487377508582 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1008438.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.3532133620298 #Duplication Level Percentage of deduplicated Percentage of total 1 82.93456969314286 50.88302349471524 2 10.331951559466484 12.677968569482074 3 2.9112295765967993 5.3583986807638535 4 1.210779354835745 2.9714081636631304 5 0.6573489339947788 2.016523470034225 6 0.40322570034885113 1.4843515455934178 7 0.26509704685351243 1.138518897407329 8 0.19861775680176386 0.974867008843709 9 0.1384140835474291 0.7642933920175713 >10 0.8482189485850731 9.642126597165145 >50 0.06335629461851425 2.648384863601993 >100 0.031182017296253393 3.742335581397447 >500 0.0030857201169511585 1.2769057868258187 >1k 0.002598501151116765 3.324339271624642 >5k 3.248126438895956E-4 1.096554676864466 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5833 0.5784192979637816 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5172 0.5128723828336497 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4509 0.44712714118270036 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4204 0.4168823467580555 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3597 0.35669024768999186 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3578 0.3548061457422271 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2589 0.25673368119805084 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2085 0.20675539795208037 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1966 0.19495496996344844 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1728 0.17135411398618458 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1429 0.14170429912399174 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1322 0.1310938302602639 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1104 0.10947623949117348 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1077 0.10679883146013935 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1077 0.10679883146013935 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1072 0.10630301515809598 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1017 0.10084903583561905 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1009 0.10005572975234968 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.916326040867164E-5 9.916326040867164E-5 14-15 0.0 0.0 0.0 9.916326040867164E-5 9.916326040867164E-5 16-17 0.0 0.0 0.0 9.916326040867164E-5 9.916326040867164E-5 18-19 0.0 0.0 0.0 9.916326040867164E-5 9.916326040867164E-5 20-21 0.0 0.0 0.0 1.9832652081734327E-4 9.916326040867164E-5 22-23 0.0 0.0 0.0 3.4707141143035074E-4 9.916326040867164E-5 24-25 0.0 0.0 0.0 5.949795624520298E-4 9.916326040867164E-5 26-27 0.0 0.0 0.0 0.0011403774946997236 9.916326040867164E-5 28-29 0.0 0.0 0.0 0.001933683577969097 9.916326040867164E-5 30-31 0.0 0.0 0.0 0.003222805963281828 9.916326040867164E-5 32-33 0.0 0.0 0.0 0.004809418129820574 1.9832652081734327E-4 34-35 0.0 0.0 0.0 0.006792683337994007 1.9832652081734327E-4 36-37 0.0 0.0 0.0 0.00877594854616744 1.9832652081734327E-4 38-39 0.0 0.0 0.0 0.011750846358427589 1.9832652081734327E-4 40-41 4.958163020433582E-5 0.0 0.0 0.015320723733139766 1.9832652081734327E-4 42-43 9.916326040867164E-5 0.0 0.0 0.023650437607468183 1.9832652081734327E-4 44-45 9.916326040867164E-5 0.0 0.0 0.034409651361809054 1.9832652081734327E-4 46-47 9.916326040867164E-5 0.0 0.0 0.04427639577247189 1.9832652081734327E-4 48-49 9.916326040867164E-5 0.0 0.0 0.058010507339072905 1.9832652081734327E-4 50-51 9.916326040867164E-5 0.0 0.0 0.07685152681672051 1.9832652081734327E-4 52-53 9.916326040867164E-5 0.0 0.0 0.10863335177769977 1.9832652081734327E-4 54-55 9.916326040867164E-5 0.0 0.0 0.133176258728846 1.9832652081734327E-4 56-57 9.916326040867164E-5 0.0 0.0 0.17993173601153467 1.9832652081734327E-4 58-59 9.916326040867164E-5 0.0 0.0 0.2441895287563539 1.9832652081734327E-4 60-61 9.916326040867164E-5 0.0 0.0 0.3097364438864858 1.9832652081734327E-4 62-63 9.916326040867164E-5 0.0 0.0 0.39491768457753473 1.9832652081734327E-4 64-65 9.916326040867164E-5 0.0 0.0 0.45039952877618655 1.9832652081734327E-4 66-67 9.916326040867164E-5 0.0 0.0 0.5650818394388153 1.9832652081734327E-4 68-69 9.916326040867164E-5 0.0 0.0 0.7048028733546337 1.9832652081734327E-4 70-71 9.916326040867164E-5 0.0 0.0 0.8076847560286304 1.9832652081734327E-4 72-73 9.916326040867164E-5 0.0 0.0 0.9812204617438058 1.9832652081734327E-4 74-75 9.916326040867164E-5 0.0 0.0 1.142906157840145 1.9832652081734327E-4 76-77 9.916326040867164E-5 0.0 0.0 1.2499528974513057 1.9832652081734327E-4 78-79 9.916326040867164E-5 0.0 0.0 1.3704858404780462 1.9832652081734327E-4 80-81 9.916326040867164E-5 0.0 0.0 1.5908266051061144 1.9832652081734327E-4 82-83 9.916326040867164E-5 0.0 0.0 1.7701633615551973 1.9832652081734327E-4 84-85 9.916326040867164E-5 0.0 0.0 2.0499029191680602 1.9832652081734327E-4 86-87 9.916326040867164E-5 0.0 0.0 2.4232525946067085 1.9832652081734327E-4 88-89 9.916326040867164E-5 0.0 0.0 2.9352325080966803 1.9832652081734327E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 4110 0.0 25.257708 12-13 GTACTTT 4465 0.0 22.878822 14-15 GTACATG 4300 0.0 22.86992 1 GCTCCGT 105 0.0021933713 22.623772 6 GTACACG 190 3.281075E-7 22.503582 1 TACATGG 4340 0.0 22.004536 2 AGTACTT 4360 0.0 21.793541 12-13 ACCGTCC 110 0.002872811 21.59649 8 ACATGGG 4275 0.0 21.560059 3 TAGACTG 450 0.0 21.11552 5 ATTCCGA 115 0.003718462 20.656488 6 ACTTTTT 5275 0.0 19.682396 16-17 AGAGTAC 7285 0.0 19.597923 10-11 TACCTGG 735 0.0 19.392767 2 AGGTGCG 125 0.006021642 19.004913 7 CTCCTAA 820 0.0 18.539537 1 TACTTTT 5065 0.0 18.433157 14-15 CTACACT 415 1.8189894E-12 18.317078 4 CATGGGA 2160 0.0 17.816221 4 GTATAGG 320 8.250936E-9 17.815336 1 >>END_MODULE