FastQCFastQC Report
Thu 26 May 2016
SRR936687_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936687_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008438
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT114471.1351218418980642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38290.3796961241048037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31630.3136533926726284No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28840.285986843018609No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28740.28499521041452225No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26780.2655592113744226No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25930.2571303342396855No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20300.2013014186296034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19880.19713656169243918No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG18480.18325370523522516No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA16400.16262774707022148No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA15500.15370305363344103No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15440.15310807407098898No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA15320.15191811494608493No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC13270.13158964656230723No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC13240.13129215678108125No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA12170.12068168791735337No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11750.11651683098018917No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA11170.11076536187648621No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA11120.11026954557444284No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA10820.1072946477621827No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT10570.10481556625196592No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG10100.10015489301275833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC600.00395264331.6635278
TAAGGTG6600.030.2272725
GGTAAGG5700.030.001493
AGGTAAG5100.029.8083552
GTAAGGT5800.029.4827584
GCGACAG1901.1912562E-824.9987587
GTACATG48650.024.3150251
GTACCGG1452.3312929E-522.9344461
AAGGTAA6500.022.6572171
GGTGTGC10300.022.5948478
TACATGG50700.022.5822542
ACATGGG47500.022.5011143
GAGTACT42750.022.22167212-13
AGTACTT43500.022.00232712-13
TCGCGTA1100.002879608221.5876989
GTACTTT44600.021.24719614-15
GTAAGCG1351.0040822E-921.10901894-95
TGCACCG2056.827522E-720.8536595
GGGCGCG3451.8189894E-1220.651157
AAGGTGT9250.020.5395226
Produced by FastQC (version 0.11.5)