##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936687_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1008438 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.997009236066074 31.0 31.0 34.0 30.0 34.0 2 32.11677465545725 33.0 31.0 34.0 30.0 34.0 3 32.23954075510839 34.0 31.0 34.0 30.0 34.0 4 35.93613786866421 37.0 35.0 37.0 35.0 37.0 5 35.81994331828035 37.0 35.0 37.0 35.0 37.0 6 35.882033402152636 37.0 35.0 37.0 35.0 37.0 7 35.81310402820996 37.0 35.0 37.0 35.0 37.0 8 35.77933100497998 37.0 35.0 37.0 33.0 37.0 9 37.39595889881183 39.0 37.0 39.0 34.0 39.0 10-11 37.30050136944463 39.0 37.0 39.0 34.0 39.0 12-13 37.19152838350003 39.0 37.0 39.0 33.0 39.0 14-15 38.28137128906289 40.0 38.0 41.0 33.0 41.0 16-17 38.27971427098146 40.0 38.0 41.0 33.5 41.0 18-19 38.43372968888519 40.0 38.0 41.0 34.0 41.0 20-21 38.434929068519835 40.0 38.0 41.0 34.0 41.0 22-23 38.343508475483866 40.0 38.0 41.0 34.0 41.0 24-25 38.219660008845366 40.0 38.0 41.0 33.5 41.0 26-27 38.01452741764987 40.0 37.0 41.0 33.0 41.0 28-29 38.05467763015673 40.0 37.0 41.0 33.0 41.0 30-31 37.9538236361581 40.0 37.0 41.0 33.0 41.0 32-33 37.77104591457284 40.0 37.0 41.0 33.0 41.0 34-35 37.58495861917143 40.0 37.0 41.0 33.0 41.0 36-37 37.394633581836466 39.5 36.0 41.0 32.0 41.0 38-39 37.19497480261553 39.0 36.0 41.0 31.5 41.0 40-41 36.99351273950406 39.0 36.0 41.0 31.0 41.0 42-43 36.724126322094165 39.0 35.0 41.0 31.0 41.0 44-45 36.437458227476554 39.0 35.0 40.0 30.0 41.0 46-47 36.04721063664796 39.0 35.0 40.0 29.5 41.0 48-49 35.62776888613877 38.0 35.0 40.0 28.0 41.0 50-51 35.286297224023684 38.0 34.5 40.0 27.0 41.0 52-53 35.03464565992158 38.0 34.0 40.0 26.5 41.0 54-55 34.70510829619669 38.0 34.0 40.0 26.0 41.0 56-57 34.33583720565865 38.0 33.5 40.0 25.5 41.0 58-59 33.973843210985706 37.0 33.0 40.0 24.5 41.0 60-61 33.536732550736886 36.5 32.5 39.0 23.0 40.5 62-63 33.244381409665245 36.0 32.0 39.0 23.0 40.0 64-65 32.92415101374601 36.0 32.0 39.0 22.0 40.0 66-67 32.45140058189001 35.0 31.0 38.5 21.5 40.0 68-69 31.987495512862466 35.0 31.0 38.0 20.0 40.0 70-71 31.452445266838417 35.0 30.5 37.0 18.0 39.5 72-73 30.877352896261346 34.0 30.0 36.5 15.5 39.0 74-75 30.348093288828863 34.0 29.0 36.0 12.5 39.0 76-77 28.99355934623646 32.5 28.0 34.5 11.0 36.5 78-79 29.768165717674265 34.0 29.0 35.0 8.0 37.0 80-81 29.593420715998406 34.0 29.0 35.0 7.0 37.0 82-83 29.202805725290006 34.0 29.0 35.0 4.5 36.5 84-85 28.771927475957867 33.5 29.0 35.0 2.0 36.0 86-87 28.390906034877702 33.0 28.0 35.0 2.0 35.5 88-89 27.876887324753728 33.0 27.0 35.0 2.0 35.0 90-91 27.290742712987807 32.5 26.0 34.5 2.0 35.0 92-93 26.717586505070216 32.0 25.0 34.0 2.0 35.0 94-95 25.994032850804906 32.0 24.5 34.0 2.0 35.0 96-97 25.14937903966332 31.5 21.5 34.0 2.0 35.0 98-99 24.028228309524234 31.0 9.5 34.0 2.0 35.0 100-101 20.2208098068498 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23614734891893363 1101 2 0.3553847531325047 1101 3 0.2005326826323639 1101 4 0.13081721352413922 1101 5 0.1642454518627119 1101 6 0.16207499350368693 1101 7 0.18335419276534282 1101 8 0.07468191354449516 1101 9 0.2568566481481298 1101 10-11 0.2302102574394027 1101 12-13 0.25067520709345104 1101 14-15 0.29996149491518764 1101 16-17 0.32272290442044493 1101 18-19 0.41185203955033245 1101 20-21 0.22419606142040038 1101 22-23 0.2344796987257638 1101 24-25 0.29037225549902246 1101 26-27 0.15220522404759151 1101 28-29 0.22012066701288546 1101 30-31 0.32196092576781155 1101 32-33 0.2551347043891212 1101 34-35 0.2928722534641466 1101 36-37 0.4157440007347759 1101 38-39 0.4052664527540344 1101 40-41 0.4225804753593465 1101 42-43 0.3941349508816856 1101 44-45 0.409274599083588 1101 46-47 0.11208233612632768 1101 48-49 0.07116599310339922 1101 50-51 0.0332585036530233 1101 52-53 0.00247720391769235 1101 54-55 0.22057667858661745 1101 56-57 0.30337931413952646 1101 58-59 0.37583673504385473 1101 60-61 0.3957746641681297 1101 62-63 0.18962785154593575 1101 64-65 0.19375392782284706 1101 66-67 0.20157334816058636 1101 68-69 0.11548744850034254 1101 70-71 0.05344045692043764 1101 72-73 0.2036801413260001 1101 74-75 0.5112453389418619 1101 76-77 0.48864554996319853 1101 78-79 0.43596697758664504 1101 80-81 0.6755200881478665 1101 82-83 0.5477250228117221 1101 84-85 0.3008236195800471 1101 86-87 0.5277902447027607 1101 88-89 0.4926274327099769 1101 90-91 0.6282697121833678 1101 92-93 0.47839750329168496 1101 94-95 0.8318609717121603 1101 96-97 0.5546636329834378 1101 98-99 0.017269326308404942 1101 100-101 0.1345131201336578 1102 1 0.43607086744444246 1102 2 0.47727828336629585 1102 3 0.38879513683789924 1102 4 0.2829752185851362 1102 5 0.25083947686554353 1102 6 0.2017202516522687 1102 7 0.37642270505903497 1102 8 0.2921772868873944 1102 9 0.4392366383282891 1102 10-11 0.44074912135904043 1102 12-13 0.3678287743404951 1102 14-15 0.6184808702220508 1102 16-17 0.6132875926404182 1102 18-19 0.45097325796631793 1102 20-21 0.47446516290470697 1102 22-23 0.4311315097315429 1102 24-25 0.41144785885288115 1102 26-27 0.49210872519953597 1102 28-29 0.5874241285077701 1102 30-31 0.6586930423218007 1102 32-33 0.5344254567402231 1102 34-35 0.5346859030468067 1102 36-37 0.695878645898496 1102 38-39 0.6301632971850495 1102 40-41 0.9014843353132704 1102 42-43 0.9341708782903808 1102 44-45 0.7920112667574131 1102 46-47 0.5380782476721961 1102 48-49 0.6387541356422091 1102 50-51 0.6085691506296413 1102 52-53 0.6460888763125965 1102 54-55 0.7946681069961841 1102 56-57 0.6411406258440309 1102 58-59 0.6238586030802722 1102 60-61 0.6811485263881707 1102 62-63 0.5833445679412961 1102 64-65 0.6213207782968411 1102 66-67 0.9161640778505102 1102 68-69 0.7410079474239346 1102 70-71 0.5795444811678969 1102 72-73 0.5535628225210019 1102 74-75 0.6177493329744195 1102 76-77 0.8699529110690669 1102 78-79 0.9319410777490518 1102 80-81 0.9848392613925689 1102 82-83 0.9764674218750606 1102 84-85 0.8615449531903501 1102 86-87 0.9023688884312797 1102 88-89 0.6466597060037778 1102 90-91 0.7911138788185603 1102 92-93 0.8050560182291768 1102 94-95 1.067901922348561 1102 96-97 1.2036868418061921 1102 98-99 1.2975762908436614 1102 100-101 1.6892953367745172 1103 1 0.3465879512353922 1103 2 0.40681204000622273 1103 3 0.3732199514291281 1103 4 0.2525879391179302 1103 5 0.27895021057272373 1103 6 0.24900100380941126 1103 7 0.28071929121016836 1103 8 0.3566788106456613 1103 9 0.20917520311044058 1103 10-11 0.3626640866774764 1103 12-13 0.30143043217009335 1103 14-15 0.4564115410393583 1103 16-17 0.43552882648457114 1103 18-19 0.3252638072708649 1103 20-21 0.3636156888861848 1103 22-23 0.3355114358832125 1103 24-25 0.3506857251549036 1103 26-27 0.26809686485676565 1103 28-29 0.4177420690945297 1103 30-31 0.5150636731843932 1103 32-33 0.504095454582469 1103 34-35 0.4180120236819107 1103 36-37 0.3688615754419615 1103 38-39 0.23665727124843272 1103 40-41 0.37836824360169174 1103 42-43 0.45760548033127435 1103 44-45 0.6563746582529717 1103 46-47 0.4364543601854436 1103 48-49 0.44823313638651285 1103 50-51 0.36039535733223715 1103 52-53 0.4494876936725305 1103 54-55 0.46998061684545434 1103 56-57 0.6351795428967861 1103 58-59 0.4576256074709235 1103 60-61 0.3158962759051036 1103 62-63 0.6359124097322777 1103 64-65 0.6785929358270195 1103 66-67 0.45104717152830176 1103 68-69 0.4945439878829312 1103 70-71 0.31589369213507723 1103 72-73 0.5681836891600263 1103 74-75 0.4741442082008014 1103 76-77 0.37069812220110876 1103 78-79 0.43889254295717706 1103 80-81 0.7142076108605266 1103 82-83 0.7045477895165959 1103 84-85 0.7868923143125741 1103 86-87 0.7040675697803351 1103 88-89 0.6341302187963187 1103 90-91 0.6951404433679684 1103 92-93 0.9078187847050394 1103 94-95 0.6906135574611838 1103 96-97 0.7077568643776964 1103 98-99 0.5735413983552924 1103 100-101 0.2614923246547569 1104 1 0.25320255288948346 1104 2 0.2789004084028974 1104 3 0.2388013776267357 1104 4 0.17515959068977338 1104 5 0.11749182172193429 1104 6 0.16532805167819475 1104 7 0.15295925927948417 1104 8 0.2010149639697758 1104 9 0.2227208707352588 1104 10-11 0.14433805500024732 1104 12-13 0.3133700907628878 1104 14-15 0.36863088918445186 1104 16-17 0.35730783679927924 1104 18-19 0.3225042194798462 1104 20-21 0.2974607895519199 1104 22-23 0.03771638205940775 1104 24-25 0.15282416881845506 1104 26-27 0.22986117710945564 1104 28-29 0.3443394025984148 1104 30-31 0.39777586239025453 1104 32-33 0.30514427559892 1104 34-35 0.4492981100297655 1104 36-37 0.28195232561161987 1104 38-39 0.3333837842546217 1104 40-41 0.32490685591987756 1104 42-43 0.21317854485943855 1104 44-45 0.22218224036848255 1104 46-47 0.4008613034504478 1104 48-49 0.3716921790108856 1104 50-51 0.34946359968800067 1104 52-53 0.3830648300731596 1104 54-55 0.40231031297101794 1104 56-57 0.3572819461069088 1104 58-59 0.5146261961040253 1104 60-61 0.31369808065182525 1104 62-63 0.41298183409173816 1104 64-65 0.4555288221935925 1104 66-67 0.37279479597327736 1104 68-69 0.5007891837722092 1104 70-71 0.21128873364141043 1104 72-73 0.4151583281929163 1104 74-75 0.7009876542982347 1104 76-77 0.587789445607033 1104 78-79 0.5873391200615998 1104 80-81 0.5959643075984111 1104 82-83 0.467042030505592 1104 84-85 0.48343596021363666 1104 86-87 0.42887063825245875 1104 88-89 0.5080001794396196 1104 90-91 0.5814791809927549 1104 92-93 0.5695442495416181 1104 94-95 0.6580057114675029 1104 96-97 0.7249631311291616 1104 98-99 0.13334273172178968 1104 100-101 0.34121953999380494 1105 1 0.15716435813225615 1105 2 0.2682869501558969 1105 3 0.19632172113933422 1105 4 0.07214664891275646 1105 5 0.1489088111823449 1105 6 0.1805837343494261 1105 7 0.1906669732992441 1105 8 0.1235774958738105 1105 9 0.1473090459747297 1105 10-11 0.15973246391235563 1105 12-13 0.16745290612487196 1105 14-15 0.2599136565027109 1105 16-17 0.36375320208168205 1105 18-19 0.3445622923261382 1105 20-21 0.26416889392690024 1105 22-23 0.21014589291427654 1105 24-25 0.20916714092307132 1105 26-27 0.2242262493251772 1105 28-29 0.23023376646953153 1105 30-31 0.2932357308705704 1105 32-33 0.26952195929108314 1105 34-35 0.03472554899922642 1105 36-37 0.22491362038986296 1105 38-39 0.04957752055464937 1105 40-41 0.1499682617992235 1105 42-43 0.13030462959654443 1105 44-45 0.117219023841443 1105 46-47 -0.0034719398009102065 1105 48-49 0.0730491138215612 1105 50-51 0.17707612708793619 1105 52-53 0.11008912217896949 1105 54-55 -0.11675883995815184 1105 56-57 0.13127708168028107 1105 58-59 0.1709607605576835 1105 60-61 -6.84407412308019E-4 1105 62-63 0.24080845631796421 1105 64-65 -0.13831257347647608 1105 66-67 0.13403702640296444 1105 68-69 0.08544522076587313 1105 70-71 -0.0134243552694997 1105 72-73 0.08756952237962423 1105 74-75 0.19844974480059463 1105 76-77 -0.155327991260517 1105 78-79 -0.1914955194603678 1105 80-81 -0.22614451142690228 1105 82-83 -0.2281650078993529 1105 84-85 -0.24860379596448112 1105 86-87 -0.19588238700051974 1105 88-89 0.05838753193038926 1105 90-91 0.2107029746449811 1105 92-93 0.01989466842279697 1105 94-95 -0.040756261928287074 1105 96-97 6.989507249919313E-4 1105 98-99 -0.0916658260657961 1105 100-101 -0.41713376151056636 1106 1 0.009484774450442046 1106 2 0.13238136517308874 1106 3 0.047534433712513646 1106 4 0.10203978152124904 1106 5 0.12002116133567853 1106 6 0.1104954525912234 1106 7 0.16916711269148976 1106 8 0.14079708513131095 1106 9 0.17298323982288366 1106 10-11 0.03912006729493811 1106 12-13 0.09943041750857162 1106 14-15 0.04523286475473043 1106 16-17 -0.01196002159640841 1106 18-19 0.00291653449693996 1106 20-21 -4.7751439946353E-4 1106 22-23 -0.06813121887829965 1106 24-25 0.11754647369883742 1106 26-27 -0.01105207053358015 1106 28-29 0.07543588523173383 1106 30-31 0.09938757549468136 1106 32-33 -0.15871798123488645 1106 34-35 -0.027316082819375254 1106 36-37 0.04358474760852715 1106 38-39 0.04132549936702645 1106 40-41 0.0011447323874591575 1106 42-43 -0.15571432774942906 1106 44-45 -0.06233399167756204 1106 46-47 -0.1880393591857299 1106 48-49 0.09354163310415942 1106 50-51 -0.18016390636620372 1106 52-53 -0.06599395560190402 1106 54-55 -0.0463220775754678 1106 56-57 -0.12407279286669137 1106 58-59 -0.047900869402361934 1106 60-61 -0.17735850833230415 1106 62-63 -0.193776619418486 1106 64-65 -0.16211147692410321 1106 66-67 -0.11394643238338631 1106 68-69 -0.040133257070756656 1106 70-71 -0.043956833663703065 1106 72-73 0.014135826626443304 1106 74-75 0.031991237226943525 1106 76-77 -0.2061383248726365 1106 78-79 -0.10187931278812101 1106 80-81 -0.18739067814003363 1106 82-83 -0.16339314654817727 1106 84-85 -0.23671456633898558 1106 86-87 -0.138471365719969 1106 88-89 -0.21627095705762756 1106 90-91 -0.07597856052833407 1106 92-93 -0.23586594862022636 1106 94-95 0.01348497959201822 1106 96-97 0.07920373652557089 1106 98-99 0.1334080518265921 1106 100-101 -0.1787702265231097 1107 1 0.15144878454649557 1107 2 0.24927410363797975 1107 3 0.11270666592184142 1107 4 0.13154714474327278 1107 5 0.08830029191256727 1107 6 0.06878747561604825 1107 7 -0.030230029179911355 1107 8 -0.011288404509329553 1107 9 0.020863249265239858 1107 10-11 0.1684049903942153 1107 12-13 0.08882320136940791 1107 14-15 0.2449350673659083 1107 16-17 0.16005701096155178 1107 18-19 0.1609697748593817 1107 20-21 -0.018864687321695328 1107 22-23 0.1257239862205708 1107 24-25 0.2354551733774315 1107 26-27 0.18824247650569959 1107 28-29 0.20475258549139852 1107 30-31 0.26619042999160314 1107 32-33 0.25573336339294883 1107 34-35 0.09333271600396387 1107 36-37 0.27437046059721126 1107 38-39 0.2176415277667303 1107 40-41 0.3220259584410954 1107 42-43 0.34869543419424787 1107 44-45 0.4364121723652161 1107 46-47 0.5467511839313417 1107 48-49 0.3528868420162894 1107 50-51 0.39052665672507914 1107 52-53 0.34539225492631687 1107 54-55 0.46709060786695744 1107 56-57 0.565435296690417 1107 58-59 0.5287610760861909 1107 60-61 0.5591352905818283 1107 62-63 0.29581409075564835 1107 64-65 0.4438826034448695 1107 66-67 0.43505122518593 1107 68-69 0.37160704181613724 1107 70-71 0.14009829516998806 1107 72-73 0.2716488552622067 1107 74-75 0.3657204231868896 1107 76-77 0.38509571845325397 1107 78-79 0.3270124814411446 1107 80-81 0.4205672307412307 1107 82-83 0.3452674915372036 1107 84-85 0.33874801832444845 1107 86-87 0.617189508424314 1107 88-89 0.6396586610716994 1107 90-91 0.5816833668018404 1107 92-93 0.4846275960766171 1107 94-95 0.4473167603840871 1107 96-97 0.4228265143941421 1107 98-99 0.7805090668798869 1107 100-101 0.6300738111736806 1108 1 -0.09047053701687346 1108 2 0.06033482637834453 1108 3 -0.04465526518869467 1108 4 0.09436433002986888 1108 5 0.10821986066417111 1108 6 0.010994866544237425 1108 7 0.08098566156859732 1108 8 0.17750636270590547 1108 9 0.21680936223026492 1108 10-11 0.06411722526106445 1108 12-13 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-1.9197418446905345 2316 54-55 -1.4941502770669217 2316 56-57 -1.632673713320699 2316 58-59 -1.4881218802850924 2316 60-61 -1.285531623273073 2316 62-63 -1.2709885294396273 2316 64-65 -1.2110055438793168 2316 66-67 -1.4630577848251924 2316 68-69 -1.6517769651051353 2316 70-71 -1.5375806073142542 2316 72-73 -1.5135445695307759 2316 74-75 -1.5355649556290807 2316 76-77 -1.5843270970207293 2316 78-79 -1.689734521401519 2316 80-81 -1.6949438309322353 2316 82-83 -1.7184081975000005 2316 84-85 -1.5553071046516962 2316 86-87 -1.8939957591569474 2316 88-89 -2.125005780900864 2316 90-91 -2.7995550462497008 2316 92-93 -2.551931543708328 2316 94-95 -2.3651044483663775 2316 96-97 -2.3794105391221123 2316 98-99 -1.6968114126652871 2316 100-101 -1.7459736489672153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 10.0 5 36.0 6 75.0 7 136.0 8 377.0 9 681.0 10 1056.0 11 1647.0 12 2474.0 13 3412.0 14 4597.0 15 6572.0 16 8289.0 17 11152.0 18 10126.0 19 6939.0 20 6498.0 21 6845.0 22 7265.0 23 7968.0 24 9194.0 25 11176.0 26 13453.0 27 16514.0 28 20346.0 29 25340.0 30 31089.0 31 38730.0 32 48199.0 33 61598.0 34 82838.0 35 114698.0 36 160591.0 37 190230.0 38 94756.0 39 3528.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.263792455393755 19.29104066851594 14.64114177699011 21.804025099100187 2 16.118593624640624 21.73875961842652 37.65494356627801 24.48770319065484 3 21.344773487719884 24.231290250283127 29.133717713000486 25.290218548996506 4 13.227439772954899 16.945184315483214 36.84346223947229 32.9839136720896 5 13.924749843807332 38.57820046213195 32.72707438737765 14.769975306683063 6 32.09637082299556 36.81902109995855 16.047887921716555 15.03672015532933 7 29.35578396227818 33.15464060648431 19.37636040002578 18.11321503121173 8 27.403921957507993 33.28150333440762 18.51094528596574 20.80362942211865 9 26.494043262947255 17.900753442452586 19.875490610230873 35.72971268436929 10-11 25.75438450355897 26.440495102326565 26.104579557692194 21.70054083642227 12-13 27.65202223636952 23.908708319202567 26.278660661339615 22.1606087830883 14-15 23.202418805838573 26.01614487449073 25.915394352516756 24.866041967153944 16-17 22.298945639756283 28.85612028750952 26.398128094059402 22.446805978674792 18-19 22.14324813718121 27.900979554113476 28.714481494776965 21.24129081392835 20-21 23.371070192740103 25.917502495214094 28.77992087704606 21.931506434999733 22-23 22.56169212690952 26.121167948355623 28.790650859995935 22.52648906473893 24-25 23.311350349527952 26.52649188368273 28.726244668081076 21.43591309870824 26-27 22.728347750895075 26.15856557862707 29.445541579547307 21.667545090930545 28-29 21.81834678978777 27.950553231829822 28.687286675036045 21.54381330334636 30-31 22.35938337475934 26.40862057595784 28.694711403778868 22.537284645503952 32-33 22.17539840736223 26.934767302333423 28.802149961820326 22.08768432848402 34-35 23.249247221300823 26.336272058411563 28.824247184114327 21.590233536173294 36-37 23.28804343784072 25.95382225529896 29.111851646545446 21.646282660314874 38-39 22.458943234559086 26.920445079138407 28.965895116823358 21.654716569479156 40-41 22.673194917880384 26.727437248218163 28.651899597149054 21.947468236752403 42-43 22.222145094018178 27.36646959308702 27.80096031225023 22.61042500064457 44-45 22.782136301294468 26.61937247178364 27.946218100715114 22.652273126206776 46-47 23.61905243554884 27.652369307780944 27.194433371213698 21.53414488545652 48-49 23.575564473823825 27.496363111688915 27.01798958169091 21.910082832796355 50-51 22.431449625602056 27.839419923662685 27.89138245114382 21.83774799959144 52-53 23.452052295048155 28.038628286290002 26.65722626810732 21.852093150554524 54-55 22.40872637124264 27.75308335915711 27.17813449023861 22.660055779361638 56-57 23.228078596903252 27.78175593384404 26.848437543390553 22.14172792586216 58-59 23.945550303098816 27.030682743349228 26.54690470311389 22.476862250438064 60-61 22.666958878896708 27.742278729808966 27.992130103198186 21.598632288096137 62-63 22.365375690673478 27.686734706020015 27.890267701668726 22.057621901637784 64-65 22.175305260506484 28.560265211954373 27.15065732778924 22.1137721997499 66-67 22.68020075028485 27.674513618869973 26.822138063916018 22.82314756692916 68-69 22.550218834275494 27.765599658472755 27.033904313863932 22.65027719338782 70-71 23.221523299321582 27.863190056893405 26.577063935413044 22.33822270837197 72-73 23.03142090727436 27.321165603105896 27.416365448406143 22.2310480412136 74-75 22.519873933412406 29.083180940064818 26.425339860128766 21.97160526639401 76-77 23.141063399793342 27.528544484176333 26.57096306521252 22.759429050817808 78-79 23.018881958011445 28.146513680494262 26.625891289903507 22.208713071590786 80-81 23.391472455027174 28.142048730953395 26.52411285240345 21.94236596161598 82-83 23.014282370861665 28.011872555475335 26.921059208073178 22.05278586558982 84-85 22.410831780548055 28.70454570246221 26.579998274499385 22.304624242490352 86-87 22.581344106494043 28.555172783092956 26.198643041870945 22.664840068542052 88-89 22.287998564038382 28.693250137472496 25.80779038927154 23.210960909217572 90-91 22.770668342054908 27.877957818101034 26.24460304654211 23.10677079330195 92-93 23.140611836993543 28.495090438763988 26.374778793313496 21.989518930928973 94-95 22.621676644212645 28.258105909080545 26.410731125877724 22.70948632082909 96-97 21.929519677719245 28.262881933684536 27.22330337775023 22.584295010845988 98-99 22.251861312855254 28.94579484107938 25.7775964173942 23.024747428671162 100-101 21.788928847144547 28.95905300752122 25.947738700443818 23.304279444890412 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 124.0 1 90.0 2 68.0 3 88.5 4 98.0 5 111.0 6 178.0 7 380.0 8 610.5 9 749.0 10 774.5 11 772.5 12 841.5 13 977.5 14 1122.5 15 1196.0 16 1280.0 17 1426.5 18 1562.5 19 1630.0 20 1764.0 21 2089.5 22 2437.5 23 2801.0 24 3359.5 25 4084.5 26 5155.5 27 6679.0 28 8238.5 29 9951.5 30 12049.5 31 14128.0 32 16684.5 33 18618.5 34 20070.5 35 21638.0 36 23803.0 37 26039.0 38 26735.0 39 26968.0 40 27166.5 41 28249.5 42 29044.5 43 30120.0 44 31818.5 45 32883.5 46 33335.0 47 38640.0 48 53527.0 49 53300.0 50 39525.5 51 33581.0 52 31582.0 53 31843.5 54 31956.5 55 30059.5 56 27043.0 57 25116.5 58 24406.5 59 22169.0 60 18355.0 61 15052.5 62 12748.5 63 10068.5 64 7161.5 65 5342.5 66 4010.5 67 3228.0 68 2471.0 69 1584.0 70 1154.5 71 945.0 72 789.5 73 719.5 74 592.5 75 469.5 76 343.0 77 215.0 78 151.0 79 129.5 80 94.0 81 56.5 82 32.0 83 21.0 84 12.0 85 5.5 86 2.5 87 0.0 88 0.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011403774946997238 2 0.008627203655554432 3 0.005949795624520298 4 3.9665304163468654E-4 5 0.006743101707789671 6 0.0 7 2.974897812260149E-4 8 0.0012891223853127311 9 0.0 10-11 0.0 12-13 0.0 14-15 6.445611926563656E-4 16-17 0.005354816062068268 18-19 0.0019832652081734326 20-21 7.437244530650373E-4 22-23 2.974897812260149E-4 24-25 6.445611926563656E-4 26-27 3.470714114303507E-4 28-29 0.0 30-31 0.001338704015517067 32-33 0.004759836499616238 34-35 7.437244530650373E-4 36-37 8.428877134737089E-4 38-39 0.007734734311876388 40-41 4.958163020433582E-5 42-43 0.0015866121665387462 44-45 0.0068422649681983425 46-47 0.0 48-49 0.002082428468582104 50-51 0.0018841019477647608 52-53 5.949795624520298E-4 54-55 4.958163020433582E-5 56-57 0.015271142102935432 58-59 0.002082428468582104 60-61 0.003817785525733858 62-63 1.9832652081734327E-4 64-65 0.00366904063512085 66-67 0.002082428468582104 68-69 0.0026278264008297984 70-71 0.006048958884928969 72-73 0.0032723875934861637 74-75 0.008131387353511075 76-77 0.0011899591249040595 78-79 0.006743101707789671 80-81 0.0023303366196037833 82-83 0.00614812214533764 84-85 0.003470714114303507 86-87 5.949795624520298E-4 88-89 0.005404397692272604 90-91 0.011354193316792901 92-93 0.004809418129820574 94-95 6.941428228607014E-4 96-97 4.958163020433582E-5 98-99 9.916326040867163E-4 100-101 0.0024294998800124547 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1008438.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.42987175319614 #Duplication Level Percentage of deduplicated Percentage of total 1 81.47921903298253 47.60820318647747 2 10.896544095544716 12.733673481114488 3 3.263369277314967 5.720347450705017 4 1.4052142175509945 3.2842594606908992 5 0.7746453572846578 2.2631214440175675 6 0.4532942859221704 1.5891556195733423 7 0.29360342510000764 1.2008647332424804 8 0.22589995677054728 1.0559444402524505 9 0.1617581728565423 0.8506358365535189 >10 0.9326314510444293 10.285803895328744 >50 0.06874006101779948 2.8088414997273286 >100 0.038276038132498594 4.255841749209075 >500 0.002721851391260094 1.073005645165821 >1k 0.003912661374936385 4.132488160979065 >5k 0.0 0.0 >10k+ 1.7011571195375588E-4 1.1378133969627746 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11447 1.1351218418980642 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3829 0.3796961241048037 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3163 0.3136533926726284 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2884 0.285986843018609 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2874 0.28499521041452225 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2678 0.2655592113744226 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2593 0.2571303342396855 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2030 0.2013014186296034 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1988 0.19713656169243918 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1848 0.18325370523522516 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1640 0.16262774707022148 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1550 0.15370305363344103 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1544 0.15310807407098898 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1532 0.15191811494608493 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1327 0.13158964656230723 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1324 0.13129215678108125 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 1217 0.12068168791735337 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1175 0.11651683098018917 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1117 0.11076536187648621 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1112 0.11026954557444284 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 1082 0.1072946477621827 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1057 0.10481556625196592 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1010 0.10015489301275833 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 9.916326040867164E-5 0.0 14-15 0.0 0.0 0.0 9.916326040867164E-5 0.0 16-17 0.0 0.0 0.0 9.916326040867164E-5 0.0 18-19 0.0 0.0 0.0 9.916326040867164E-5 0.0 20-21 0.0 0.0 0.0 1.9832652081734327E-4 9.916326040867164E-5 22-23 0.0 0.0 0.0 2.479081510216791E-4 9.916326040867164E-5 24-25 0.0 0.0 0.0 5.45397932247694E-4 9.916326040867164E-5 26-27 0.0 0.0 0.0 0.0011403774946997236 9.916326040867164E-5 28-29 9.916326040867164E-5 0.0 0.0 0.001933683577969097 9.916326040867164E-5 30-31 9.916326040867164E-5 0.0 0.0 0.003222805963281828 9.916326040867164E-5 32-33 9.916326040867164E-5 0.0 0.0 0.004809418129820574 9.916326040867164E-5 34-35 9.916326040867164E-5 0.0 0.0 0.0068422649681983425 9.916326040867164E-5 36-37 9.916326040867164E-5 0.0 0.0 0.00877594854616744 9.916326040867164E-5 38-39 9.916326040867164E-5 0.0 0.0 0.011800427988631923 1.9832652081734327E-4 40-41 9.916326040867164E-5 0.0 0.0 0.01517197884252676 1.9832652081734327E-4 42-43 9.916326040867164E-5 0.0 0.0 0.023501692716855177 1.9832652081734327E-4 44-45 9.916326040867164E-5 0.0 0.0 0.03450881462221773 1.9832652081734327E-4 46-47 9.916326040867164E-5 0.0 0.0 0.04442514066308489 1.9832652081734327E-4 48-49 9.916326040867164E-5 0.0 0.0 0.057812180818255565 1.9832652081734327E-4 50-51 9.916326040867164E-5 0.0 0.0 0.07630612888447282 1.9832652081734327E-4 52-53 9.916326040867164E-5 0.0 0.0 0.10803837221524774 1.9832652081734327E-4 54-55 9.916326040867164E-5 0.0 0.0 0.13228378938516794 1.9832652081734327E-4 56-57 9.916326040867164E-5 0.0 0.0 0.1794359197094913 1.9832652081734327E-4 58-59 9.916326040867164E-5 0.0 0.0 0.24359454919390183 1.9832652081734327E-4 60-61 9.916326040867164E-5 0.0 0.0 0.30839773987096875 1.9832652081734327E-4 62-63 9.916326040867164E-5 0.0 0.0 0.3938764703432437 2.974897812260149E-4 64-65 9.916326040867164E-5 0.0 0.0 0.44950705943250846 2.974897812260149E-4 66-67 9.916326040867164E-5 0.0 0.0 0.5646356047669763 2.974897812260149E-4 68-69 9.916326040867164E-5 0.0 0.0 0.7049020366150422 2.974897812260149E-4 70-71 9.916326040867164E-5 0.0 0.0 0.8086268070025128 2.974897812260149E-4 72-73 9.916326040867164E-5 0.0 0.0 0.9825591657593229 2.974897812260149E-4 74-75 9.916326040867164E-5 0.0 0.0 1.1449390046785226 2.974897812260149E-4 76-77 9.916326040867164E-5 0.0 0.0 1.2509445300553925 2.974897812260149E-4 78-79 9.916326040867164E-5 0.0 0.0 1.3703370955874332 2.974897812260149E-4 80-81 9.916326040867164E-5 0.0 0.0 1.5897853908718236 2.974897812260149E-4 82-83 9.916326040867164E-5 0.0 0.0 1.7673372086335501 2.974897812260149E-4 84-85 9.916326040867164E-5 0.0 0.0 2.0458868071215086 2.974897812260149E-4 86-87 9.916326040867164E-5 0.0 0.0 2.416311166378102 2.974897812260149E-4 88-89 9.916326040867164E-5 0.0 0.0 2.922787518915392 2.974897812260149E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTACGC 60 0.003952643 31.663527 8 TAAGGTG 660 0.0 30.227272 5 GGTAAGG 570 0.0 30.00149 3 AGGTAAG 510 0.0 29.808355 2 GTAAGGT 580 0.0 29.482758 4 GCGACAG 190 1.1912562E-8 24.998758 7 GTACATG 4865 0.0 24.315025 1 GTACCGG 145 2.3312929E-5 22.934446 1 AAGGTAA 650 0.0 22.657217 1 GGTGTGC 1030 0.0 22.594847 8 TACATGG 5070 0.0 22.582254 2 ACATGGG 4750 0.0 22.501114 3 GAGTACT 4275 0.0 22.221672 12-13 AGTACTT 4350 0.0 22.002327 12-13 TCGCGTA 110 0.0028796082 21.587698 9 GTACTTT 4460 0.0 21.247196 14-15 GTAAGCG 135 1.0040822E-9 21.109018 94-95 TGCACCG 205 6.827522E-7 20.853659 5 GGGCGCG 345 1.8189894E-12 20.65115 7 AAGGTGT 925 0.0 20.539522 6 >>END_MODULE