FastQCFastQC Report
Thu 26 May 2016
SRR936686_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936686_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078389
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58750.5447941327294696No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58500.5424758598242378No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49860.4623563482194273No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42580.3948482412190777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37780.35033740143862746No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34890.323538166654148No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27560.255566405072752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21870.2028025137496766No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC19790.18351448317814814No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT18820.17451958430584882No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15740.14595846211339322No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC15410.14289834187848727No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA12190.11303898685910187No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11860.10997886662419591No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT11450.10617689905961579No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA11190.10376589523817471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG48850.027.1459561
TACATGG50200.025.8478532
ACATGGG48050.025.421743
CCGTAGT950.001221705625.0168194
GAGTACT45050.024.73619812-13
GTACTTT46900.023.70375314-15
CATGGGA23650.022.710834
AGTACTT46950.021.96387912-13
ATGGGTA4500.021.1262955
CATGGGT7600.020.6388764
AGAGTAC77400.020.14087110-11
ACTTTTT57850.019.62720916-17
CCTTATA3506.366463E-1119.01192
TACTTTT54450.018.9337214-15
TAAGGTG5050.018.825415
CGACCCG1559.57763E-418.400325
CGTCTAT1559.5806166E-418.3994676
CATGGGG23400.018.0783944
GTATTGA2653.9791848E-717.9357551
ATGGGAT11350.017.5897355
Produced by FastQC (version 0.11.5)