##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936686_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1078389 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.485562260000798 31.0 31.0 34.0 30.0 34.0 2 31.537476736131396 33.0 31.0 34.0 30.0 34.0 3 31.57356019024675 33.0 31.0 34.0 28.0 34.0 4 35.20743905956014 37.0 35.0 37.0 33.0 37.0 5 35.140562450099175 37.0 35.0 37.0 32.0 37.0 6 35.26181275958861 37.0 35.0 37.0 32.0 37.0 7 35.09716159938575 37.0 35.0 37.0 32.0 37.0 8 35.091566215901686 37.0 35.0 37.0 32.0 37.0 9 36.62155029400337 39.0 37.0 39.0 32.0 39.0 10-11 36.485568287510354 39.0 37.0 39.0 32.0 39.0 12-13 36.410532748386714 39.0 37.0 39.0 32.0 39.0 14-15 37.49437447896817 40.0 37.0 41.0 32.0 41.0 16-17 37.445853490716246 40.0 37.0 41.0 32.0 41.0 18-19 37.42631833225302 40.0 37.0 41.0 32.0 41.0 20-21 37.33467514969088 40.0 37.0 41.0 31.5 41.0 22-23 37.24083099883252 39.5 37.0 41.0 31.0 41.0 24-25 37.08633990146413 39.0 36.0 41.0 31.0 41.0 26-27 36.89537263455024 39.0 36.0 41.0 30.5 41.0 28-29 37.00539369374131 39.0 36.0 41.0 31.0 41.0 30-31 36.9298657534526 39.0 36.0 41.0 30.5 41.0 32-33 36.75372662369516 39.0 36.0 41.0 30.0 41.0 34-35 36.566384208295894 39.0 35.0 41.0 30.0 41.0 36-37 36.32057912311791 39.0 35.0 40.5 30.0 41.0 38-39 36.12923861426628 39.0 35.0 40.0 30.0 41.0 40-41 35.983579209357664 39.0 35.0 40.0 29.0 41.0 42-43 35.70253081216518 38.0 35.0 40.0 28.0 41.0 44-45 35.3983655248709 38.0 35.0 40.0 27.0 41.0 46-47 35.00122219347564 38.0 34.0 40.0 25.5 41.0 48-49 34.706909102374006 38.0 34.0 40.0 24.0 41.0 50-51 33.98569347424723 37.5 33.5 39.5 23.5 40.5 52-53 34.15477299935367 38.0 33.5 39.5 24.0 40.5 54-55 34.50125511295089 38.0 34.0 40.0 23.5 41.0 56-57 34.3928563811389 38.0 34.0 40.0 23.0 41.0 58-59 34.15070721233246 38.0 34.0 40.0 23.0 41.0 60-61 33.809729605921426 37.5 33.0 40.0 22.0 41.0 62-63 33.49174602114822 37.0 33.0 40.0 21.5 41.0 64-65 33.185486869765924 36.5 33.0 39.5 20.5 41.0 66-67 32.78466073003341 36.0 32.0 39.0 19.5 41.0 68-69 32.394966473137245 35.5 32.0 39.0 18.0 40.5 70-71 31.969981147804738 35.0 31.0 38.5 16.0 40.0 72-73 31.5634094005039 35.0 31.0 37.5 13.0 40.0 74-75 31.15060938121587 35.0 31.0 37.0 9.5 39.0 76-77 30.742443125810816 35.0 30.5 36.5 7.5 39.0 78-79 30.28645090037083 34.5 30.0 36.0 6.0 38.5 80-81 29.84932107059697 34.0 29.5 35.5 2.0 37.5 82-83 29.448396636093285 34.0 29.0 35.0 2.0 37.0 84-85 29.05724604015805 34.0 29.0 35.0 2.0 36.5 86-87 28.765954585961097 34.0 29.0 35.0 2.0 36.0 88-89 28.376432344914498 34.0 28.5 35.0 2.0 36.0 90-91 28.00589444068884 34.0 27.5 35.0 2.0 35.5 92-93 27.639256335144367 34.0 27.0 35.0 2.0 35.0 94-95 27.232810701889576 33.5 26.0 35.0 2.0 35.0 96-97 26.68896520643293 33.0 25.0 35.0 2.0 35.0 98-99 26.05027128429537 33.0 24.5 35.0 2.0 35.0 100-101 23.99047699855989 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17938175548512447 1101 2 0.27869148078159967 1101 3 0.32983974972733066 1101 4 0.2945100918562886 1101 5 0.29869400144908553 1101 6 0.13016814995778248 1101 7 0.17817045449412916 1101 8 0.19734178483368225 1101 9 0.3141781544251785 1101 10-11 0.19697636592594137 1101 12-13 0.30754209674142174 1101 14-15 0.405719345432594 1101 16-17 0.5604121622692801 1101 18-19 0.6065363769543026 1101 20-21 0.4838503892854291 1101 22-23 0.4879924477620321 1101 24-25 0.37739797964463406 1101 26-27 0.35144682699493757 1101 28-29 0.514617434007306 1101 30-31 0.4940442525065052 1101 32-33 0.38284946206972137 1101 34-35 0.3440351617052926 1101 36-37 0.4009862264836457 1101 38-39 0.3049570060766129 1101 40-41 0.4288144086670158 1101 42-43 0.24417719058321552 1101 44-45 0.3222804539950701 1101 46-47 0.4964291289025837 1101 48-49 0.6116507240907012 1101 50-51 0.5613442933480073 1101 52-53 0.5961977767526676 1101 54-55 0.660924597115816 1101 56-57 0.5475527216215994 1101 58-59 0.5890414253752425 1101 60-61 0.5506790123600851 1101 62-63 0.6546057340774638 1101 64-65 0.7051315510971108 1101 66-67 0.6658345168229189 1101 68-69 0.7261421856008425 1101 70-71 0.6681231101046059 1101 72-73 0.768542193209683 1101 74-75 0.5619291431998903 1101 76-77 0.47329604619222465 1101 78-79 0.6059908085650925 1101 80-81 0.5551602172049073 1101 82-83 0.6452212082565083 1101 84-85 0.579294219069471 1101 86-87 0.4340830143223151 1101 88-89 0.5669054316464859 1101 90-91 0.39585778466189225 1101 92-93 0.17640814255022264 1101 94-95 -0.09906736606535915 1101 96-97 0.07386213177397849 1101 98-99 0.07835943290996639 1101 100-101 0.07915074929319488 1102 1 0.0050332446031085 1102 2 0.11069319899465668 1102 3 0.17266621020842976 1102 4 0.008169885670717747 1102 5 0.0428449178293846 1102 6 0.06416729085125183 1102 7 -0.04739512397093648 1102 8 0.021310856998624672 1102 9 0.29450461490628044 1102 10-11 0.2888613888354499 1102 12-13 0.2987541528697122 1102 14-15 0.2924604679984597 1102 16-17 0.3205424600928737 1102 18-19 0.3545031581685052 1102 20-21 0.08594446145038148 1102 22-23 -0.28547905853807976 1102 24-25 0.014389674948183995 1102 26-27 0.07502214200066248 1102 28-29 0.13091540078852404 1102 30-31 0.22240550680203341 1102 32-33 0.17054634064933083 1102 34-35 0.022099308326147593 1102 36-37 -0.11560526836171192 1102 38-39 -0.1425444257676034 1102 40-41 -0.26177121561706684 1102 42-43 0.08234657775388854 1102 44-45 0.21725826040972862 1102 46-47 0.2472703373790992 1102 48-49 0.2518748077104007 1102 50-51 0.20975852587950783 1102 52-53 0.15087990733686496 1102 54-55 0.0611644310218864 1102 56-57 0.2496754306708553 1102 58-59 0.19618274839930194 1102 60-61 -0.3246753690832165 1102 62-63 0.06367288757117251 1102 64-65 0.26325368740868527 1102 66-67 0.19800089712074254 1102 68-69 0.08738574230187623 1102 70-71 0.1909001719602763 1102 72-73 0.023313814057331683 1102 74-75 0.023197757174685307 1102 76-77 0.2625321573033368 1102 78-79 0.09160435381136978 1102 80-81 0.33866895145462195 1102 82-83 0.25779638007781713 1102 84-85 0.11933216294117699 1102 86-87 0.18505666838875356 1102 88-89 -0.006938354149621517 1102 90-91 -0.21999631041256507 1102 92-93 -0.07684930303970106 1102 94-95 -0.06241215415241541 1102 96-97 -0.2505185956028839 1102 98-99 -0.3236737858758296 1102 100-101 -0.23939521290611765 1103 1 0.17588910575339867 1103 2 0.47568497144411737 1103 3 0.4238121904623071 1103 4 0.31669127730212665 1103 5 0.3436568987101225 1103 6 0.2678491197181998 1103 7 0.2768007309107432 1103 8 0.2027518537076105 1103 9 0.5879813426918901 1103 10-11 0.4499680923301952 1103 12-13 0.514207745566786 1103 14-15 0.6982941931199136 1103 16-17 0.6817211546548592 1103 18-19 0.4046402840108527 1103 20-21 0.49983873742593943 1103 22-23 0.5623430892444006 1103 24-25 0.5002666926570143 1103 26-27 0.5820811844704963 1103 28-29 0.4599326982340557 1103 30-31 0.44322561728628784 1103 32-33 0.4639021038525968 1103 34-35 0.4537792957889195 1103 36-37 0.6398440146660249 1103 38-39 0.44781660698588155 1103 40-41 0.5555703060879935 1103 42-43 0.5333010715636419 1103 44-45 0.7498976390337688 1103 46-47 0.7898621137464303 1103 48-49 0.6731523308259 1103 50-51 0.2763189200183618 1103 52-53 0.757197490787739 1103 54-55 0.9813420089877241 1103 56-57 0.8315723708056311 1103 58-59 0.9013822664999083 1103 60-61 1.1650005241936938 1103 62-63 0.9508961872107236 1103 64-65 0.8585987547962404 1103 66-67 1.107222610018404 1103 68-69 0.8222191344034258 1103 70-71 0.8386008654252528 1103 72-73 0.864674396254479 1103 74-75 0.870292648257589 1103 76-77 0.7694408510817397 1103 78-79 0.7527228109497486 1103 80-81 1.0316926742253507 1103 82-83 0.8932938530444332 1103 84-85 0.777188983972362 1103 86-87 0.9721354961586961 1103 88-89 0.9884429536391259 1103 90-91 1.095448467051785 1103 92-93 0.9480321810537049 1103 94-95 0.6773308328402194 1103 96-97 0.8775525335304799 1103 98-99 0.9189175007563577 1103 100-101 0.9634888373994777 1104 1 0.2950876015717334 1104 2 0.26128302993081576 1104 3 0.3390647719041162 1104 4 0.31993496263261534 1104 5 0.30840184019459116 1104 6 0.2923924359433556 1104 7 0.4510377761888762 1104 8 0.3763007636350153 1104 9 0.4195424650198305 1104 10-11 0.5703618829897081 1104 12-13 0.5134355285110601 1104 14-15 0.6187179328819639 1104 16-17 0.3930259532378031 1104 18-19 0.6275139244230274 1104 20-21 0.6007351818642093 1104 22-23 0.5838762804307223 1104 24-25 0.3562229872536804 1104 26-27 0.3994202200560153 1104 28-29 0.3229371751177297 1104 30-31 0.36099290902257763 1104 32-33 0.48088073588862557 1104 34-35 0.5759684310446218 1104 36-37 0.5026771250271338 1104 38-39 0.34094609067616233 1104 40-41 0.15266492283023325 1104 42-43 0.2232165520999274 1104 44-45 0.33991444469773313 1104 46-47 0.6232431378669432 1104 48-49 0.19197568877693527 1104 50-51 0.20557897966030936 1104 52-53 0.07429335674387261 1104 54-55 0.4690890356984738 1104 56-57 0.2763237505463749 1104 58-59 0.2926240491815406 1104 60-61 0.3165189597080982 1104 62-63 0.05998539282812487 1104 64-65 0.17940596051828095 1104 66-67 -0.058196533277104834 1104 68-69 0.08097724495138436 1104 70-71 0.11109872734761339 1104 72-73 -0.10835753130392689 1104 74-75 -0.4320707683531104 1104 76-77 -0.11650965655821466 1104 78-79 -0.10046946442397697 1104 80-81 0.006300533168811739 1104 82-83 -0.08228294745183362 1104 84-85 -0.030207590191970723 1104 86-87 -0.16128028173320885 1104 88-89 -0.11807548721379746 1104 90-91 -0.17399869707250204 1104 92-93 -0.44504311664891816 1104 94-95 -0.3110285222237401 1104 96-97 -0.17191153235113887 1104 98-99 -0.2958939053139673 1104 100-101 -0.21709597621673993 1105 1 0.3426636897403732 1105 2 0.2725176988647533 1105 3 0.17027922657631578 1105 4 0.3439908781362959 1105 5 0.44986568079811917 1105 6 0.4769027416176854 1105 7 0.4014969042615064 1105 8 0.10483255094510469 1105 9 0.3315467037605231 1105 10-11 0.47022045396058587 1105 12-13 0.3637853839253964 1105 14-15 0.3289742811100851 1105 16-17 0.3311095987436019 1105 18-19 0.23503273294983273 1105 20-21 0.08697638532671448 1105 22-23 -0.07495495054465806 1105 24-25 0.013516612680923856 1105 26-27 0.008949244278312563 1105 28-29 0.11842000155061783 1105 30-31 0.20799673179553935 1105 32-33 0.16888546553025208 1105 34-35 -0.06295741636425589 1105 36-37 -0.0641933756623061 1105 38-39 -0.18923133578752527 1105 40-41 -0.16374196559541332 1105 42-43 0.03151383981330014 1105 44-45 0.18804315042445552 1105 46-47 0.13476898421377115 1105 48-49 0.32054659475465286 1105 50-51 0.20653718050841263 1105 52-53 0.31247058023891583 1105 54-55 0.29417489910883887 1105 56-57 0.2671553496974397 1105 58-59 0.34075682608181523 1105 60-61 0.30751075068814515 1105 62-63 0.14848739566851066 1105 64-65 0.1898387852696004 1105 66-67 -0.10831621987934881 1105 68-69 0.25455497456999865 1105 70-71 0.23197983550925372 1105 72-73 0.19539774278340616 1105 74-75 0.19466545441206762 1105 76-77 0.16809989134681302 1105 78-79 0.13258280059408278 1105 80-81 0.4401923449767473 1105 82-83 0.2385503096699182 1105 84-85 -0.06606312371336642 1105 86-87 0.1367930501329262 1105 88-89 0.059286368722119676 1105 90-91 0.1714232682658796 1105 92-93 0.1713001080627521 1105 94-95 0.14526949476072204 1105 96-97 0.20025243799891612 1105 98-99 -0.06072020485911622 1105 100-101 0.05158702273950411 1106 1 -0.14066052178329613 1106 2 -0.07283338088058144 1106 3 -0.09253924137822622 1106 4 0.05569497381826238 1106 5 0.23769450195378283 1106 6 0.06646760275795316 1106 7 0.28801250192184114 1106 8 0.2664763031608217 1106 9 0.18960225526429042 1106 10-11 0.23099111263980632 1106 12-13 -0.01767886629672688 1106 14-15 0.15909775214998234 1106 16-17 0.026010433151363088 1106 18-19 0.1273017297262271 1106 20-21 0.06193527376391472 1106 22-23 -0.0037388790787176163 1106 24-25 0.14501812174322737 1106 26-27 0.14549892981369794 1106 28-29 -0.05009586627593876 1106 30-31 0.013511928011695318 1106 32-33 -0.08069422304291862 1106 34-35 -0.07051889979247505 1106 36-37 0.07044453500145664 1106 38-39 0.1933287999805131 1106 40-41 0.2637650152842994 1106 42-43 0.0955384794806804 1106 44-45 0.1877308103600015 1106 46-47 0.24586025917640342 1106 48-49 0.33625206790387807 1106 50-51 0.5989047943000827 1106 52-53 0.40274795724193524 1106 54-55 0.48248963895968444 1106 56-57 0.5362868921119528 1106 58-59 0.6540304226602203 1106 60-61 0.4799532246244027 1106 62-63 0.43235536429117616 1106 64-65 0.4619944771202782 1106 66-67 0.281469277014061 1106 68-69 0.5368339725703848 1106 70-71 0.5932691372437056 1106 72-73 0.6760212433132722 1106 74-75 0.5041725786194036 1106 76-77 0.4933394419068975 1106 78-79 0.61659955090024 1106 80-81 0.5243266112502987 1106 82-83 0.6095262756568758 1106 84-85 0.8912252444889539 1106 86-87 0.8788546975000457 1106 88-89 0.8336414419809266 1106 90-91 0.6825647713160343 1106 92-93 0.464333117175471 1106 94-95 0.5187559138342017 1106 96-97 0.4966197169366673 1106 98-99 0.5540474870651302 1106 100-101 0.9081934431828422 1107 1 0.3480819244710922 1107 2 0.347340613736133 1107 3 0.46976467005441336 1107 4 0.4691984885310063 1107 5 0.36471647998559575 1107 6 0.33313731285345227 1107 7 0.38164090463192224 1107 8 0.42722394830775556 1107 9 0.5322108855333383 1107 10-11 0.6197985450510686 1107 12-13 0.6218117711315401 1107 14-15 0.7329170136530223 1107 16-17 0.7205768385307181 1107 18-19 0.7063458424369315 1107 20-21 0.8589470742116561 1107 22-23 0.6360865355213292 1107 24-25 0.6958321942001149 1107 26-27 0.7608238471711815 1107 28-29 0.5024878705354894 1107 30-31 0.6284595497063208 1107 32-33 0.5198347069859679 1107 34-35 0.30697279567488067 1107 36-37 0.6080711617813037 1107 38-39 0.7159195456295322 1107 40-41 0.5892807645809555 1107 42-43 0.7781558461807307 1107 44-45 0.748965493633051 1107 46-47 0.9303045783031862 1107 48-49 1.0152652092505576 1107 50-51 0.8185284653734541 1107 52-53 0.984997069053037 1107 54-55 1.0647432264014185 1107 56-57 1.1579640720349929 1107 58-59 1.099954063030765 1107 60-61 0.9423453954932413 1107 62-63 0.9847090536877801 1107 64-65 0.7535692814680885 1107 66-67 0.4765418762186542 1107 68-69 0.8094670129289341 1107 70-71 0.9133183512782104 1107 72-73 0.9414896941453392 1107 74-75 0.6943336207610464 1107 76-77 0.9565743740250099 1107 78-79 0.5734491007860676 1107 80-81 0.7757254696064386 1107 82-83 0.9064315560954164 1107 84-85 0.893187360960976 1107 86-87 1.021580320753987 1107 88-89 1.0718604632321167 1107 90-91 1.1554746741858999 1107 92-93 1.1175057574663505 1107 94-95 1.106124699532952 1107 96-97 1.5075912778844618 1107 98-99 1.5270137828810455 1107 100-101 1.719959160031177 1108 1 0.16829006810855418 1108 2 -0.06783949272547574 1108 3 -0.18027833607937893 1108 4 -0.09241825976160811 1108 5 0.1239396066979026 1108 6 -0.007932082812054375 1108 7 0.16744918298496714 1108 8 -0.004025226105554225 1108 9 0.3604303710932655 1108 10-11 0.24637774742593166 1108 12-13 0.24180493451753904 1108 14-15 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-1.8893081613730551 2316 72-73 -1.9388087704837318 2316 74-75 -1.927647653453331 2316 76-77 -2.1681894610541512 2316 78-79 -2.3290327234833192 2316 80-81 -2.5904826185936862 2316 82-83 -2.5130822431105884 2316 84-85 -2.455652740440467 2316 86-87 -2.490909515186125 2316 88-89 -2.5872070739563853 2316 90-91 -2.683417204132372 2316 92-93 -2.979536501107333 2316 94-95 -2.8947793039108696 2316 96-97 -2.903160503815446 2316 98-99 -3.3972848969869425 2316 100-101 -3.9065600679785817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2450.0 3 975.0 4 945.0 5 1048.0 6 1185.0 7 1342.0 8 1654.0 9 2157.0 10 2541.0 11 3266.0 12 4089.0 13 5042.0 14 6024.0 15 7617.0 16 9663.0 17 12151.0 18 10417.0 19 7202.0 20 6980.0 21 7313.0 22 8207.0 23 9352.0 24 10515.0 25 11978.0 26 13877.0 27 16717.0 28 19444.0 29 23459.0 30 28502.0 31 35537.0 32 44735.0 33 57493.0 34 76114.0 35 105905.0 36 147964.0 37 193708.0 38 154710.0 39 26080.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.981942896148006 17.710522459624244 14.597132750782288 22.710401893445468 2 15.436054895538415 19.538289906977305 39.68687026992439 25.33878492755989 3 20.10763368194182 23.657245241758456 29.949076637296006 26.286044439003714 4 12.419852171783512 15.909343900972905 37.898234521450675 33.77256940579291 5 13.567179521434507 36.768847969362305 34.30801715948225 15.355955349720935 6 31.687254635424544 35.530043155401195 17.313379102245175 15.469323106929082 7 28.117060501356583 32.7733228207685 20.55144586415602 18.558170813718895 8 26.53771092741277 32.30732699128834 19.94488431456939 21.210077766729494 9 25.968520251489263 16.956419718850658 21.072474279296284 36.0025857503638 10-11 25.210999675759773 25.420433813941774 26.948676807639266 22.419889702659187 12-13 26.962365042757718 23.23337978020918 27.36674972679218 22.437505450240923 14-15 23.244671082775138 24.52159855459323 26.988593677829364 25.245136684802265 16-17 21.83773611330749 28.024599237499693 27.455483328827214 22.6821813203656 18-19 21.642660131558625 26.616203240128584 30.038091059569634 21.703045568743164 20-21 22.65480544885128 25.08984260327204 29.601752284383547 22.653599663493132 22-23 21.90090462738144 24.95743039080571 30.242043934804503 22.89962104700835 24-25 22.529068621746024 25.158262109781255 30.00644037414447 22.30622889432825 26-27 21.886998917329908 25.0930933994218 30.829112001873227 22.19079568137506 28-29 21.335137280503275 26.236071648478827 30.376927898988846 22.05186317202905 30-31 21.56273590067457 25.326991952251387 30.158886929039223 22.95138521803482 32-33 21.325879446030953 25.528451213343185 30.394482009081354 22.75118733154451 34-35 22.62391113942562 25.352536505547523 29.695506199903836 22.32804615512302 36-37 22.21046525592345 24.941471482736347 30.504010635057018 22.344052626283187 38-39 21.92788849424573 25.520758951341428 30.210325225116613 22.341027329296224 40-41 21.93613660147902 25.701802339410683 30.32665228356139 22.035408775548905 42-43 21.555160414604348 25.894549914107074 29.348303353881178 23.201986317407403 44-45 21.858140125781965 25.48420121405004 29.467976787274175 23.189681872893818 46-47 22.59741042552945 26.12488843037476 29.02122431000707 22.256476834088723 48-49 22.427338237571483 26.051686453935368 28.661695662132423 22.859279646360722 50-51 21.69240976854623 26.50198747287017 29.44770370727559 22.357899051308006 52-53 22.45490116438385 26.688189662007368 28.20300607901749 22.653903094591293 54-55 21.637862364366928 26.0562479077199 28.902722537461667 23.40316719045151 56-57 22.469808516024692 26.162892595934405 28.1995832587607 23.1677156292802 58-59 22.977959245747734 25.910497920001184 28.210459157651485 22.901083676599594 60-61 22.23976906174844 26.0052198498957 29.526369793306372 22.22864129504949 62-63 21.97466478668707 26.312836702815005 29.185431301845682 22.527067208652234 64-65 21.562359740197117 26.851197563698815 28.791419531212327 22.79502316489174 66-67 21.844277782285644 26.369657698593933 28.175868504329483 23.610196014790944 68-69 21.815261446409387 26.1493241560819 28.837444595451156 23.197969802057557 70-71 22.452656081908223 26.095865637867714 28.253143895834103 23.198334384389955 72-73 22.150527363700814 25.851833682559832 29.074949308448065 22.922689645291285 74-75 21.716358186975878 27.1922088592511 28.266207818775484 22.825225134997538 76-77 21.85301496761522 26.378491813737785 28.338131507847784 23.430361710799207 78-79 22.0371237877024 26.332611327300643 28.598210600038033 23.03205428495893 80-81 22.553377585286576 26.537722911910233 27.96573186618306 22.94316763662014 82-83 22.226008897915086 26.242543533246625 28.773140259240453 22.758307309597832 84-85 22.034937288064075 26.606562994555798 28.192619239388538 23.165880477991585 86-87 22.013869954799308 26.905495862940064 28.16237937052885 22.918254811731778 88-89 21.892082251220753 26.724088764993663 27.477846541482414 23.905982442303173 90-91 21.88269455307949 26.262822516250434 28.065880908373437 23.78860202229664 92-93 22.390300091723805 26.3655856736506 28.456869871837082 22.787244362788513 94-95 21.930182081888393 26.00600834528465 28.515065144503073 23.54874442832389 96-97 21.102134894864204 26.082087943220316 29.311671095527537 23.504106066387937 98-99 21.44865592856477 26.745683588197455 27.989047636935844 23.816612846301933 100-101 21.22044952211314 26.278512601787234 28.42078164246502 24.080256233634607 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 52.0 1 46.0 2 78.5 3 114.5 4 98.5 5 112.0 6 206.5 7 478.5 8 908.5 9 1179.5 10 1082.5 11 963.0 12 1010.0 13 1095.5 14 1213.0 15 1296.5 16 1395.0 17 1494.5 18 1567.5 19 1704.0 20 1923.5 21 2246.0 22 2708.0 23 3201.0 24 3730.0 25 4669.0 26 5990.0 27 7435.0 28 8822.5 29 10421.0 30 12589.5 31 15208.5 32 18248.5 33 20233.5 34 21504.5 35 23179.0 36 25189.0 37 27273.0 38 28370.0 39 28762.5 40 28953.0 41 29888.0 42 30809.0 43 31831.0 44 33354.5 45 35209.0 46 36951.5 47 42924.0 48 56443.0 49 55419.5 50 41754.5 51 35891.0 52 34385.5 53 34443.0 54 34188.0 55 31978.0 56 29509.0 57 27058.5 58 25395.5 59 22961.5 60 19064.5 61 16243.5 62 13783.0 63 10504.5 64 7651.5 65 5978.5 66 4363.5 67 3337.0 68 2560.5 69 1772.0 70 1304.5 71 1008.5 72 839.0 73 757.0 74 618.0 75 487.5 76 339.5 77 201.5 78 137.0 79 100.0 80 69.5 81 43.0 82 25.0 83 19.0 84 12.0 85 10.0 86 10.0 87 5.5 88 2.5 89 3.0 90 3.0 91 4.0 92 4.0 93 2.0 94 1.0 95 1.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013260521017925813 2 0.004636545810463571 3 0.009458553453345685 4 0.03533047907573241 5 0.04645818902084498 6 0.06083148103328206 7 0.02911750768971123 8 0.027170158449316525 9 0.015949717587994683 10-11 0.044928128903392005 12-13 0.042192566875218496 14-15 0.019937146984993357 16-17 0.020910821605190706 18-19 0.029163873147815864 20-21 0.023507287259050304 22-23 0.01437329201243707 24-25 0.0032919475254291355 26-27 0.0033846784416384067 28-29 0.0012055019107205284 30-31 0.006954818715695357 32-33 0.0023646383633364213 34-35 0.0023646383633364213 36-37 0.006352067760335093 38-39 0.005471124056347014 40-41 0.0037556021064754927 42-43 0.0010664055364066212 44-45 0.0013909637431390714 46-47 0.0024573692795456926 48-49 1.8546183241854283E-4 50-51 8.809437039880785E-4 52-53 6.954818715695357E-4 54-55 0.0018546183241854284 56-57 0.002040080156603971 58-59 0.0021328110728132425 60-61 5.100200391509928E-4 62-63 7.882127877788071E-4 64-65 0.0030601202349059566 66-67 0.0024573692795456926 68-69 0.0065375295927536355 70-71 0.010107669866810585 72-73 0.004961104017196021 74-75 0.0033383129835337713 76-77 0.002550100195754964 78-79 0.03468136266226751 80-81 0.022672709013166863 82-83 0.004311987603731121 84-85 0.007928493335892706 86-87 0.008067589710206612 88-89 0.005424758598242378 90-91 0.010200400783019856 92-93 0.014095099263809256 94-95 0.005146565849614564 96-97 0.00421925668752185 98-99 0.024620058253561564 100-101 0.043258972411625116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1078389.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.97210592116831 #Duplication Level Percentage of deduplicated Percentage of total 1 82.80215420016305 50.48621716393252 2 10.320671318284154 12.585461295919703 3 2.984634121664426 5.459382834061694 4 1.2513132406867344 3.051808138068478 5 0.6459490409324586 1.9692436671705478 6 0.41504970646731076 1.518387279916481 7 0.2742544633513124 1.1705310532166573 8 0.1922160212244171 0.9375852484672555 9 0.14235038891934773 0.7811462691998975 >10 0.8675446591301137 9.762929243350689 >50 0.06385707109669396 2.6545244232839242 >100 0.03435610043600094 4.036283274933532 >500 0.003053874402182671 1.3169076582689718 >1k 0.002290405801637003 3.177988187699636 >5k 3.0538744021826707E-4 1.091604262509982 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5875 0.5447941327294696 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5850 0.5424758598242378 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4986 0.4623563482194273 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4258 0.3948482412190777 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3778 0.35033740143862746 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3489 0.323538166654148 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2756 0.255566405072752 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2187 0.2028025137496766 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1979 0.18351448317814814 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1882 0.17451958430584882 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1574 0.14595846211339322 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1541 0.14289834187848727 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1219 0.11303898685910187 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1186 0.10997886662419591 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1145 0.10617689905961579 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1119 0.10376589523817471 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.273091620927142E-5 0.0 10-11 0.0 0.0 0.0 9.273091620927142E-5 0.0 12-13 0.0 0.0 0.0 9.273091620927142E-5 0.0 14-15 0.0 0.0 0.0 1.8546183241854283E-4 0.0 16-17 0.0 0.0 0.0 1.8546183241854283E-4 0.0 18-19 0.0 0.0 0.0 2.781927486278143E-4 0.0 20-21 0.0 0.0 0.0 2.781927486278143E-4 0.0 22-23 0.0 0.0 0.0 2.781927486278143E-4 0.0 24-25 0.0 0.0 0.0 3.7092366483708567E-4 0.0 26-27 0.0 0.0 0.0 6.027509553602642E-4 0.0 28-29 0.0 0.0 0.0 0.001112770994511257 0.0 30-31 0.0 0.0 0.0 0.002689196570068871 0.0 32-33 0.0 0.0 0.0 0.004358353061835757 0.0 34-35 0.0 0.0 0.0 0.006212971386021185 0.0 36-37 0.0 0.0 0.0 0.00853124429125297 0.0 38-39 0.0 0.0 0.0 0.010988613570798663 0.0 40-41 0.0 0.0 0.0 0.013863271973286077 0.0 42-43 0.0 0.0 0.0 0.02322909451042249 0.0 44-45 0.0 0.0 0.0 0.032734013421872814 0.0 46-47 0.0 0.0 0.0 0.04279531783057876 0.0 48-49 0.0 0.0 0.0 0.054201220524319145 0.0 50-51 0.0 0.0 0.0 0.06834268524623305 0.0 52-53 0.0 0.0 0.0 0.09389005266188732 9.273091620927142E-5 54-55 0.0 0.0 0.0 0.11280715956857869 9.273091620927142E-5 56-57 0.0 0.0 0.0 0.15564884285726208 1.3909637431390714E-4 58-59 0.0 0.0 0.0 0.2090154851356978 1.8546183241854283E-4 60-61 0.0 0.0 0.0 0.2622893964979242 1.8546183241854283E-4 62-63 0.0 0.0 0.0 0.33740143862743405 1.8546183241854283E-4 64-65 0.0 0.0 0.0 0.38492603318468566 1.8546183241854283E-4 66-67 0.0 0.0 0.0 0.48957287212684847 1.8546183241854283E-4 68-69 0.0 0.0 0.0 0.6097521395340643 1.8546183241854283E-4 70-71 0.0 0.0 0.0 0.696084622524896 1.8546183241854283E-4 72-73 0.0 0.0 0.0 0.8499715779741819 1.8546183241854283E-4 74-75 0.0 0.0 0.0 0.9934726708080295 1.8546183241854283E-4 76-77 0.0 0.0 0.0 1.0805470011285352 1.8546183241854283E-4 78-79 0.0 0.0 0.0 1.184683820031547 1.8546183241854283E-4 80-81 0.0 0.0 0.0 1.378398703992715 1.8546183241854283E-4 82-83 0.0 0.0 0.0 1.5328420449392568 1.8546183241854283E-4 84-85 0.0 0.0 0.0 1.7761216036142802 1.8546183241854283E-4 86-87 0.0 0.0 0.0 2.1259953504718614 1.8546183241854283E-4 88-89 0.0 0.0 0.0 2.599108484971564 1.8546183241854283E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4885 0.0 27.145956 1 TACATGG 5020 0.0 25.847853 2 ACATGGG 4805 0.0 25.42174 3 CCGTAGT 95 0.0012217056 25.016819 4 GAGTACT 4505 0.0 24.736198 12-13 GTACTTT 4690 0.0 23.703753 14-15 CATGGGA 2365 0.0 22.71083 4 AGTACTT 4695 0.0 21.963879 12-13 ATGGGTA 450 0.0 21.126295 5 CATGGGT 760 0.0 20.638876 4 AGAGTAC 7740 0.0 20.140871 10-11 ACTTTTT 5785 0.0 19.627209 16-17 CCTTATA 350 6.366463E-11 19.0119 2 TACTTTT 5445 0.0 18.93372 14-15 TAAGGTG 505 0.0 18.82541 5 CGACCCG 155 9.57763E-4 18.40032 5 CGTCTAT 155 9.5806166E-4 18.399467 6 CATGGGG 2340 0.0 18.078394 4 GTATTGA 265 3.9791848E-7 17.935755 1 ATGGGAT 1135 0.0 17.589735 5 >>END_MODULE