FastQCFastQC Report
Thu 26 May 2016
SRR936686_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936686_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078389
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT113891.0561124047073922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40770.3780639453851996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34830.32298178115689236No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT31640.2934006188861348No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31400.29117507689711225No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29270.27142339174453745No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC26630.2469424298652898No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21040.19510584770430708No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20230.1875946434913561No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT18710.17349954422754682No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG18300.1696975766629667No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA16700.15486063006948328No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA15530.14401111287299853No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA15280.14169283996776671No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC13940.12926689719572437No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC13370.12398123497179589No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12700.1177682635857747No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA10810.10024212042222241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGCG504.959054E-528.49666294-95
GTCCTAC2006.366463E-1026.3986071
TAAGGTG6300.024.878045
GTAAGGT5800.022.929414
GAGTACT42500.022.34721212-13
GTACATG49000.022.2355611
GGTAAGG6100.021.8027463
GTACTTT43900.021.47228614-15
TACATGG50950.021.2536412
ACATGGG50200.020.6269873
AGGTAAG6750.020.4050182
GGACCGA1200.004766540619.7893496
GTTCGAC857.973668E-519.55381210-11
GTGTAGC3950.019.4420131
AGTACTT44100.019.27510612-13
AAGGTGT8500.018.9977746
AAGGTAA6650.018.7659281
TACCTGG8200.018.5344142
ACTTTTT52900.018.31288716-17
CCGACCA1300.007567943518.2670929
Produced by FastQC (version 0.11.5)