##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936686_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1078389 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.5716239687163 31.0 31.0 34.0 30.0 34.0 2 31.795010891246108 31.0 31.0 34.0 30.0 34.0 3 32.067549835912644 33.0 31.0 34.0 30.0 34.0 4 35.84207646776812 37.0 35.0 37.0 35.0 37.0 5 35.669556162015745 37.0 35.0 37.0 33.0 37.0 6 35.786276566248354 37.0 35.0 37.0 35.0 37.0 7 35.7223988746176 37.0 35.0 37.0 33.0 37.0 8 35.70105592694288 37.0 35.0 37.0 33.0 37.0 9 37.34069431346202 39.0 37.0 39.0 34.0 39.0 10-11 37.22266733062003 39.0 37.0 39.0 33.0 39.0 12-13 37.11781323808014 39.0 37.0 39.0 33.0 39.0 14-15 38.227812505505895 40.0 38.0 41.0 33.0 41.0 16-17 38.16740665937802 40.0 37.5 41.0 33.0 41.0 18-19 38.23522587860225 40.0 37.5 41.0 33.5 41.0 20-21 38.30056640043621 40.0 38.0 41.0 34.0 41.0 22-23 38.22423494675854 40.0 38.0 41.0 33.5 41.0 24-25 38.10492085879956 40.0 37.0 41.0 33.0 41.0 26-27 37.89853707706588 40.0 37.0 41.0 33.0 41.0 28-29 37.84289713637658 40.0 37.0 41.0 33.0 41.0 30-31 37.85236218099406 40.0 37.0 41.0 33.0 41.0 32-33 37.69250752743213 40.0 37.0 41.0 33.0 41.0 34-35 37.52008180721428 40.0 36.0 41.0 32.5 41.0 36-37 37.286997549121885 39.5 36.0 41.0 31.5 41.0 38-39 37.11939337289235 39.0 36.0 41.0 31.0 41.0 40-41 36.91980213077099 39.0 35.5 41.0 31.0 41.0 42-43 36.67051546334393 39.0 35.0 41.0 30.5 41.0 44-45 36.40167230934291 39.0 35.0 40.0 30.0 41.0 46-47 35.99812034432844 39.0 35.0 40.0 29.5 41.0 48-49 35.51732816265745 38.0 35.0 40.0 28.0 41.0 50-51 35.29487504045386 38.0 34.5 40.0 27.0 41.0 52-53 35.04252176162777 38.0 34.0 40.0 26.5 41.0 54-55 34.77947521719899 38.0 34.0 40.0 26.0 41.0 56-57 34.46289743311551 38.0 34.0 40.0 25.5 41.0 58-59 34.08596480490806 37.0 33.0 40.0 24.5 41.0 60-61 33.678408255277084 37.0 33.0 39.5 23.0 41.0 62-63 33.41070847347293 36.0 32.5 39.0 23.0 40.5 64-65 32.97713209240821 36.0 32.0 39.0 22.0 40.0 66-67 32.54929807332975 35.0 31.5 38.5 22.0 40.0 68-69 32.08471525581214 35.0 31.0 38.0 20.5 40.0 70-71 31.584895617444168 35.0 31.0 37.0 19.5 39.5 72-73 31.15227714674389 34.0 30.0 37.0 18.0 39.0 74-75 30.787966123541693 34.0 30.0 36.0 17.0 39.0 76-77 29.383667210997146 32.5 28.5 35.0 13.5 37.0 78-79 30.189806739497527 34.0 30.0 35.0 13.5 37.0 80-81 30.021396267951545 34.0 30.0 35.0 9.5 37.0 82-83 29.678329433998307 34.0 29.5 35.0 7.0 36.5 84-85 29.248974164239435 34.0 29.0 35.0 4.5 36.0 86-87 28.900673133720765 34.0 29.0 35.0 2.0 36.0 88-89 28.473469684872526 33.0 29.0 35.0 2.0 35.0 90-91 28.015708617205853 33.0 27.0 35.0 2.0 35.0 92-93 27.56799077141922 33.0 27.0 35.0 2.0 35.0 94-95 27.042369682925177 33.0 25.5 34.5 2.0 35.0 96-97 26.348758657590167 32.0 25.0 34.0 2.0 35.0 98-99 25.517849774061123 32.0 23.5 34.0 2.0 35.0 100-101 22.689575839516166 28.5 8.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.323312029566587 1101 2 0.38124127026125265 1101 3 0.1563932106018342 1101 4 0.10115808704777152 1101 5 0.18513389280888504 1101 6 0.11604937023956552 1101 7 0.17919433738392598 1101 8 0.12180539000035395 1101 9 0.1003407340236464 1101 10-11 0.2565142146750219 1101 12-13 0.26908583314587986 1101 14-15 0.29916176402757344 1101 16-17 0.24516477469928333 1101 18-19 0.16544033258086444 1101 20-21 0.0489908628314808 1101 22-23 -0.006598548994737996 1101 24-25 -0.0497385123832359 1101 26-27 -0.012682927473882444 1101 28-29 0.14884843400874814 1101 30-31 0.020865666006784522 1101 32-33 0.05723202585875953 1101 34-35 0.1707490123425046 1101 36-37 0.23565818799674076 1101 38-39 0.26409768394466226 1101 40-41 0.37383839925802675 1101 42-43 0.5382622603334042 1101 44-45 0.4441895755902152 1101 46-47 0.5518504143998655 1101 48-49 0.4218618249547248 1101 50-51 0.37864153684265744 1101 52-53 0.34789727894185063 1101 54-55 0.2833483002369377 1101 56-57 0.26138510049093355 1101 58-59 0.46735780499837887 1101 60-61 0.3228501935809689 1101 62-63 0.27510269986795777 1101 64-65 0.3506120377514108 1101 66-67 0.31793185177832584 1101 68-69 0.11324190799930989 1101 70-71 0.22552228833475851 1101 72-73 0.4765218988352977 1101 74-75 0.2049332497584544 1101 76-77 0.7039442459967766 1101 78-79 0.35946260299536803 1101 80-81 0.37552051849804613 1101 82-83 0.24349419343316825 1101 84-85 0.21499510603452165 1101 86-87 0.21164753265183478 1101 88-89 -0.01144467703233687 1101 90-91 0.11946665808581258 1101 92-93 0.1260476750062729 1101 94-95 0.1239139760222372 1101 96-97 -0.084155343302033 1101 98-99 -0.09148532701416556 1101 100-101 -0.04585643615468982 1102 1 0.3953337935986596 1102 2 0.45321291974578415 1102 3 0.12289521518373903 1102 4 0.07675230239714637 1102 5 0.11184494664622946 1102 6 -0.026190034113234617 1102 7 0.10412990439424163 1102 8 -0.05109695822415716 1102 9 -0.05303841832458289 1102 10-11 0.03474302338063495 1102 12-13 0.06891759145057819 1102 14-15 0.32325254295083283 1102 16-17 0.25909303930409777 1102 18-19 0.061230710587736326 1102 20-21 0.08046279868485584 1102 22-23 -0.03241112058694995 1102 24-25 0.005280101591566222 1102 26-27 -0.3281647430523478 1102 28-29 -0.19357711009205048 1102 30-31 -0.06901477500123576 1102 32-33 0.19561976927227676 1102 34-35 0.22032661829897648 1102 36-37 0.15081425901620804 1102 38-39 0.1189301581714659 1102 40-41 0.17640855962458346 1102 42-43 0.07183900718334968 1102 44-45 -0.019219645486529657 1102 46-47 0.25290281989814645 1102 48-49 0.23601560502344654 1102 50-51 0.32371957235238824 1102 52-53 0.29500137401630866 1102 54-55 0.4722765648417493 1102 56-57 0.4111452665848603 1102 58-59 0.46117223798807316 1102 60-61 0.5130420607058213 1102 62-63 0.432942046946998 1102 64-65 0.31471928288313933 1102 66-67 0.1870398128321611 1102 68-69 0.2743494394998791 1102 70-71 0.276213153168662 1102 72-73 0.23900042117206866 1102 74-75 -0.12646351427362035 1102 76-77 0.2196408382075461 1102 78-79 0.46056870265172734 1102 80-81 0.4203086055541725 1102 82-83 0.4753885233300714 1102 84-85 0.3469717669738124 1102 86-87 0.2503359633505724 1102 88-89 0.32150921758393025 1102 90-91 0.4415600143286511 1102 92-93 0.4766597884083339 1102 94-95 0.18237617533495154 1102 96-97 0.21876919850781817 1102 98-99 -0.14921728577705196 1102 100-101 -0.2511399413093294 1103 1 0.4688371629764987 1103 2 0.5547555854721615 1103 3 0.34173532645156257 1103 4 0.26136776235640014 1103 5 0.15251353307468918 1103 6 0.014849771559852343 1103 7 0.16497557905338311 1103 8 0.24638286468761095 1103 9 0.05741448874327659 1103 10-11 0.2194211694392436 1103 12-13 0.2630696640097412 1103 14-15 0.22704407249165115 1103 16-17 0.17514117047475253 1103 18-19 0.22179723621068348 1103 20-21 0.07147909707069289 1103 22-23 0.15311965436995223 1103 24-25 0.17562076136275806 1103 26-27 0.05490658197508225 1103 28-29 0.07623757412672205 1103 30-31 0.19471005261199537 1103 32-33 0.09819577616774922 1103 34-35 0.03844975730540767 1103 36-37 0.15495839786591858 1103 38-39 0.10010963630968917 1103 40-41 0.32966529838025593 1103 42-43 0.2738273318973441 1103 44-45 0.25488805706792306 1103 46-47 0.17070665158596654 1103 48-49 0.4916837996637895 1103 50-51 0.10411204610258551 1103 52-53 0.3968491447418998 1103 54-55 0.3007247900415635 1103 56-57 0.3143104248672586 1103 58-59 0.33634854264251857 1103 60-61 0.04461430643532793 1103 62-63 0.06751832809415248 1103 64-65 0.2170734397551044 1103 66-67 0.1274321886353249 1103 68-69 0.07315301652543127 1103 70-71 0.08825427133196229 1103 72-73 0.268569650133049 1103 74-75 0.1582092446983303 1103 76-77 0.13940801811665082 1103 78-79 0.1699201953644689 1103 80-81 0.10263896129778516 1103 82-83 0.21501887123792685 1103 84-85 0.314182529393495 1103 86-87 0.27828221002037523 1103 88-89 0.24766597201113427 1103 90-91 0.06815655092166395 1103 92-93 0.022997059212283233 1103 94-95 0.21922972493532455 1103 96-97 0.038144590295939196 1103 98-99 0.20997406354123527 1103 100-101 0.5283323200917067 1104 1 0.3509839744380905 1104 2 0.3998963035459404 1104 3 0.2204975156295248 1104 4 0.11840056970305568 1104 5 -0.05154715950231292 1104 6 0.16048462313295886 1104 7 0.16579877898419682 1104 8 -5.381433532676283E-4 1104 9 0.06309414118420875 1104 10-11 0.0017088110835530301 1104 12-13 0.04726075529951146 1104 14-15 0.10062816327212687 1104 16-17 0.052992074126194666 1104 18-19 0.1772350002672738 1104 20-21 0.06757904058914477 1104 22-23 0.04168336801595984 1104 24-25 0.103195716866189 1104 26-27 0.15840608922960087 1104 28-29 0.06459746304277303 1104 30-31 0.1577402611666514 1104 32-33 0.08356555949853828 1104 34-35 0.06845251198220836 1104 36-37 0.1254646711618932 1104 38-39 0.04779560101779623 1104 40-41 0.018447944770507263 1104 42-43 -0.1203565096809811 1104 44-45 0.1231477739768394 1104 46-47 0.24044855545201926 1104 48-49 0.16148400017863906 1104 50-51 0.03729698634852241 1104 52-53 -0.2553221918849431 1104 54-55 -0.09136288128492254 1104 56-57 -0.1971956893407878 1104 58-59 -0.27084895807551135 1104 60-61 -0.3713752578239067 1104 62-63 -0.6617289724827273 1104 64-65 -0.5566231870470517 1104 66-67 -0.442624687966898 1104 68-69 -0.3361999654122201 1104 70-71 -0.44276894162484126 1104 72-73 -0.43140867547188577 1104 74-75 -0.427432109193294 1104 76-77 -0.2382301454748479 1104 78-79 -0.15959200262205542 1104 80-81 0.03569257392829783 1104 82-83 -0.13617626344762002 1104 84-85 -0.07078755887360799 1104 86-87 -0.23057706549962376 1104 88-89 -0.11201073783704629 1104 90-91 -0.30351421394959033 1104 92-93 -0.16746161583087726 1104 94-95 -0.37036480372132274 1104 96-97 -0.5401069159677512 1104 98-99 -0.34439770465164443 1104 100-101 -0.24118851562171528 1105 1 0.412756067782432 1105 2 0.42784756510821964 1105 3 0.21460139427787084 1105 4 0.2008294436459508 1105 5 0.07283427287814703 1105 6 0.14105037465094483 1105 7 0.16516686204557374 1105 8 0.13025155221145468 1105 9 0.12632418668104606 1105 10-11 0.17993387381797987 1105 12-13 0.19019936377239333 1105 14-15 0.23131578448542456 1105 16-17 0.34042341732435233 1105 18-19 0.21521226423804052 1105 20-21 0.214045114437873 1105 22-23 0.18120307574109518 1105 24-25 0.13038673101911513 1105 26-27 0.14569399731312416 1105 28-29 0.20278666404841772 1105 30-31 0.15037527219717362 1105 32-33 0.2630712718270516 1105 34-35 0.1991398381829299 1105 36-37 0.24617290195376995 1105 38-39 0.2571559960514378 1105 40-41 0.37202376270762727 1105 42-43 0.3462793380052105 1105 44-45 -0.04740283159547687 1105 46-47 0.07186391368873757 1105 48-49 0.30334315763452224 1105 50-51 0.1560604942368684 1105 52-53 0.21452457268262748 1105 54-55 0.25969293922469916 1105 56-57 0.26640447893439045 1105 58-59 0.38829616628113683 1105 60-61 0.29545199402183897 1105 62-63 0.19228375908001993 1105 64-65 0.3053837953106111 1105 66-67 0.2968957277884243 1105 68-69 -0.006315722446942118 1105 70-71 -0.010013518261146714 1105 72-73 -0.14324009584533925 1105 74-75 -0.18801819297804556 1105 76-77 0.050721692542005314 1105 78-79 0.10347189179176297 1105 80-81 -0.04263415013994987 1105 82-83 0.07433792577226583 1105 84-85 0.15191271483906021 1105 86-87 -0.014081201280934152 1105 88-89 0.1331394029134536 1105 90-91 -0.07455347835342963 1105 92-93 -0.054539061942406875 1105 94-95 0.05830823561034748 1105 96-97 -0.0059609803265132655 1105 98-99 0.027581882838173755 1105 100-101 0.4925110069889307 1106 1 0.2667762490728478 1106 2 0.28498163414773714 1106 3 0.05763608400210529 1106 4 0.03098620981962341 1106 5 -0.16816985762101666 1106 6 -0.22039715824306683 1106 7 -0.017332327772834333 1106 8 -0.06768960355459797 1106 9 -0.1417916956051144 1106 10-11 -0.10134944113433164 1106 12-13 -0.09810985859914467 1106 14-15 -0.1916110543731051 1106 16-17 -0.09639668811657742 1106 18-19 -0.11776983185870193 1106 20-21 -0.15229870569991277 1106 22-23 -0.07076688634050043 1106 24-25 -0.14744625678471124 1106 26-27 -0.13274813289051224 1106 28-29 -0.28559523684370447 1106 30-31 -0.2814771992847511 1106 32-33 -0.2203157248060137 1106 34-35 -0.3951291497147693 1106 36-37 -0.25957415523205185 1106 38-39 -0.287777531006725 1106 40-41 -0.3826339094107709 1106 42-43 -0.5073457410310951 1106 44-45 -0.3136024996588489 1106 46-47 -0.4971870227923887 1106 48-49 -0.4043271533166646 1106 50-51 -0.29034702424827685 1106 52-53 -0.017220339662380013 1106 54-55 -0.4132714755705109 1106 56-57 -0.5673402342740133 1106 58-59 -0.630365597650659 1106 60-61 -0.3987911822197745 1106 62-63 -0.7472874757001406 1106 64-65 -0.4158614173247983 1106 66-67 -0.45540718563091076 1106 68-69 -0.3232194398961923 1106 70-71 -0.23852246069988325 1106 72-73 -0.11816156689410917 1106 74-75 -0.2553739315053356 1106 76-77 -0.17596901849618618 1106 78-79 -0.22890359867727028 1106 80-81 -0.20154294493854863 1106 82-83 -0.11060449267209904 1106 84-85 0.019415205632160593 1106 86-87 0.06213643347081543 1106 88-89 -0.1772425905708488 1106 90-91 -0.16588393287322845 1106 92-93 0.06573881607310028 1106 94-95 0.06443136925955173 1106 96-97 0.0984192640534296 1106 98-99 -0.17498349136425517 1106 100-101 -0.22166136794344737 1107 1 0.544096169836287 1107 2 0.47658338179199333 1107 3 0.2813316838306008 1107 4 0.14554355652255424 1107 5 0.08357899505107014 1107 6 0.13978906379655598 1107 7 0.09869123552735459 1107 8 0.1037091473864038 1107 9 0.14905179069631913 1107 10-11 0.3242163457128626 1107 12-13 0.28655716790822083 1107 14-15 0.321430485745104 1107 16-17 0.3466652340235683 1107 18-19 0.2522923167483526 1107 20-21 0.3156938217871712 1107 22-23 0.25963714673978444 1107 24-25 0.10620831941334075 1107 26-27 0.13418742966492658 1107 28-29 0.21972046966592274 1107 30-31 0.06885994997126943 1107 32-33 0.24626608390373406 1107 34-35 0.2544231529524481 1107 36-37 0.3304003426245714 1107 38-39 0.23516365652130133 1107 40-41 0.41390168393700577 1107 42-43 0.3872818014627768 1107 44-45 0.5170633578137966 1107 46-47 0.6503794425604141 1107 48-49 0.5138140098639283 1107 50-51 0.18404341723687878 1107 52-53 0.04900646202511183 1107 54-55 0.0540456792531856 1107 56-57 0.34568528308651025 1107 58-59 0.1360897297372432 1107 60-61 0.41393590008976133 1107 62-63 0.2946925970020047 1107 64-65 0.32040891434628094 1107 66-67 0.09577881173204617 1107 68-69 0.3336075528112161 1107 70-71 0.21442478683202992 1107 72-73 0.30542643877383213 1107 74-75 0.6065913537131813 1107 76-77 0.36560824744847054 1107 78-79 0.5584475530367641 1107 80-81 0.49874644933855095 1107 82-83 0.41596195567330696 1107 84-85 0.6207032026173778 1107 86-87 0.6968981195661961 1107 88-89 0.7700859502571973 1107 90-91 0.733707979125132 1107 92-93 0.9326313230617984 1107 94-95 0.7777694646638835 1107 96-97 0.8519203261205774 1107 98-99 1.2699246284143655 1107 100-101 0.9876526879536023 1108 1 0.2724063060744726 1108 2 0.3031269894066462 1108 3 0.056527076880449556 1108 4 -0.0022049488504336523 1108 5 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732.0 10 1210.0 11 1971.0 12 2777.0 13 3775.0 14 5083.0 15 6949.0 16 8827.0 17 11447.0 18 10180.0 19 7177.0 20 6405.0 21 6716.0 22 7056.0 23 7843.0 24 9279.0 25 11010.0 26 13557.0 27 16504.0 28 20808.0 29 25852.0 30 31913.0 31 39395.0 32 49504.0 33 63486.0 34 84419.0 35 115867.0 36 164128.0 37 208884.0 38 127100.0 39 7833.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.154860954950905 19.352559778127578 14.722846863053743 21.769732403867774 2 15.9528877141731 21.725997542370916 37.74361827919594 24.577496464260047 3 21.228594373934705 24.176000474804745 29.23944069484707 25.35596445641348 4 13.318804021218977 16.89431316541158 36.795544756464 32.99133805690544 5 13.878758036293027 38.450039827928514 32.90120726379813 14.769994871980332 6 32.07896222977052 36.78858000220699 16.06730038974804 15.065157378274444 7 29.278581291166734 33.053842351878586 19.472472363868697 18.195103993085983 8 27.297385266355644 33.28974980271497 18.631402953850603 20.78146197707877 9 26.46065732941494 17.734410427947584 19.925013887175854 35.879918355461626 10-11 25.766388735753587 26.36144495637228 26.145056661091974 21.727109646782157 12-13 27.643297217699008 23.786383008713006 26.384566593841917 22.185753179746065 14-15 23.286830302808312 25.86201540539425 25.93198091411398 24.91917337768346 16-17 22.20451061722625 28.78826657171021 26.482651436540987 22.52457137452255 18-19 22.124344740163338 27.701413868279445 28.838016708256482 21.33622468330074 20-21 23.294330709975714 25.843874520233424 28.823457954411623 22.038336815379235 22-23 22.568637869687503 26.002316444063627 28.909787578926476 22.519258107322393 24-25 23.295026191847285 26.397941744583818 28.789286611788512 21.51774545178039 26-27 22.68991059812368 26.01746679537718 29.599847550373752 21.692775056125388 28-29 21.841329983892642 27.792753820745574 28.82869725117745 21.537218944184332 30-31 22.335086103013897 26.21471760872821 28.87150595541403 22.578690332843866 32-33 22.184015649857166 26.714879280677817 28.966980526239894 22.134124543225127 34-35 23.23970665216341 26.282098539612946 28.829395244805045 21.648799563418596 36-37 23.270971573924626 25.889303047177727 29.179210809763173 21.660514569134477 38-39 22.503902814245517 26.74931158854161 29.084879892543363 21.661905704669515 40-41 22.627728765117432 26.573666515355182 28.793772334479684 22.004832385047703 42-43 22.18560427952304 27.233362176339277 27.926429004007414 22.654604540130265 44-45 22.758704788329073 26.473517487471852 28.11525150875579 22.65252621544328 46-47 23.64747633703737 27.434199483852062 27.37698365964281 21.541340519467752 48-49 23.507137223042218 27.365731034991242 27.092558303667357 22.03457343829918 50-51 22.428925065363856 27.724475408399492 28.014167462222495 21.832432064014153 52-53 23.39654765509193 27.900249311807606 26.80076430274067 21.9024387303598 54-55 22.332873488598555 27.56309340258054 27.364996927928033 22.73903618089287 56-57 23.223925137270285 27.63470385692175 26.935624618049665 22.205746387758303 58-59 24.021320880079784 26.984934338978068 26.561196684469614 22.432548096472534 60-61 22.662911454679417 27.630251960566564 28.066021003900964 21.640815580853058 62-63 22.422223077433113 27.47825046726199 28.012999411604607 22.086527043700297 64-65 22.20752118201928 28.36874104372748 27.23870871998924 22.185029054263996 66-67 22.646999092137392 27.555577605561055 26.89345921619649 22.903964086105066 68-69 22.51897159511623 27.613007742571437 27.116758014389063 22.75126264792327 70-71 23.267394674955675 27.74931934686974 26.602919575361472 22.380366402813117 72-73 23.035946692680792 27.194883847518774 27.478362389806644 22.290807069993793 74-75 22.611552973926848 28.945677301975447 26.461276960354752 21.981492763742956 76-77 23.145013293124762 27.417220508226713 26.710139403937582 22.72762679471094 78-79 22.984847754151147 28.062862622605394 26.704743585938772 22.247546037304687 80-81 23.460942021458145 28.030165313784554 26.604213349755888 21.90467931500141 82-83 23.132445085826003 27.827416835771285 26.97096532458812 22.06917275381459 84-85 22.522563461474256 28.52552926294547 26.659522652491162 22.292384623089106 86-87 22.671680292767572 28.478945042552294 26.27572254040416 22.573652124275974 88-89 22.370103622112545 28.565862726790776 25.870370550744965 23.193663100351717 90-91 22.88250048234613 27.767831965449176 26.266807907508273 23.08285964469642 92-93 23.223395090521468 28.334568229807587 26.466041583400024 21.97599509627092 94-95 22.720593383693913 28.040124623188277 26.512846028041608 22.726435965076202 96-97 22.057982035955913 28.099307684103632 27.361316559472858 22.4813937204676 98-99 22.313309573028622 28.620780157827035 25.927157323450828 23.138752945693515 100-101 21.793236295613085 28.647087546794985 26.278877486052508 23.280798671539422 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 125.0 1 90.5 2 68.5 3 98.0 4 99.0 5 108.5 6 206.0 7 409.5 8 643.0 9 823.5 10 866.0 11 869.0 12 958.5 13 1045.5 14 1166.0 15 1317.5 16 1475.0 17 1594.0 18 1613.0 19 1764.5 20 1992.5 21 2245.0 22 2643.0 23 3145.0 24 3715.0 25 4442.0 26 5545.5 27 7083.5 28 8762.5 29 10676.0 30 12890.5 31 15125.5 32 17882.5 33 19798.0 34 21366.5 35 23095.5 36 25355.0 37 27579.0 38 28365.0 39 28609.5 40 28707.0 41 29829.0 42 30852.0 43 32003.0 44 33635.0 45 34874.5 46 35571.5 47 41668.0 48 57124.0 49 56245.0 50 41733.0 51 35874.5 52 33862.0 53 34220.0 54 34440.5 55 32082.5 56 29043.5 57 27160.0 58 26378.0 59 24177.0 60 20019.0 61 16469.5 62 13939.5 63 10891.5 64 7718.5 65 5839.5 66 4469.5 67 3481.0 68 2565.0 69 1712.0 70 1310.0 71 1040.0 72 868.5 73 792.0 74 618.0 75 462.5 76 350.0 77 233.0 78 161.0 79 114.0 80 86.5 81 65.5 82 46.5 83 28.5 84 14.5 85 8.5 86 4.0 87 3.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0301477481687962 2 0.010571324447856943 3 0.004729276726672843 4 0.010107669866810585 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0051929313077191995 10-11 3.2455820673244997E-4 12-13 9.273091620927142E-5 14-15 4.636545810463571E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001112770994511257 24-25 0.0 26-27 0.0 28-29 0.0 30-31 4.636545810463571E-5 32-33 0.0037556021064754927 34-35 9.736746201973499E-4 36-37 0.0028282929443827786 38-39 4.636545810463571E-5 40-41 0.003662871190266221 42-43 0.012796866436879457 44-45 0.0015764255755576142 46-47 0.0014836946593483427 48-49 0.012379577313937734 50-51 2.3182729052317855E-4 52-53 0.0019009837822900643 54-55 0.011730460900472835 56-57 0.0038946984807893994 58-59 8.809437039880785E-4 60-61 0.006073875011707278 62-63 0.003013754776801321 64-65 0.021699034392969514 66-67 0.0029673893186966853 68-69 4.636545810463571E-5 70-71 0.0012055019107205284 72-73 4.636545810463571E-5 74-75 0.0 76-77 0.001112770994511257 78-79 0.012333211855833099 80-81 0.018267990493226468 82-83 0.014929677509692698 84-85 0.008994898872299328 86-87 0.011823191816682108 88-89 0.03574776819867413 90-91 0.03013754776801321 92-93 0.0024110038214410568 94-95 0.009133995246613234 96-97 0.011498633609949656 98-99 0.011127709945112571 100-101 0.013770541057076808 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1078389.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.33706560452786 #Duplication Level Percentage of deduplicated Percentage of total 1 81.19603471697346 47.36738404111602 2 11.149078236242467 13.008090169953812 3 3.2330317863326816 5.658167622624406 4 1.4451387572576129 3.372206179591329 5 0.7591680332457071 2.21438176801576 6 0.45260526416306507 1.5842197793061235 7 0.32343827550021353 1.320790792780992 8 0.2117610765455121 0.9882815851936788 9 0.1756957740857753 0.922461830935216 >10 0.9350632479550354 10.13805706301115 >50 0.07379786400270408 2.9159384563140356 >100 0.03848077582875764 4.269416777771535 >500 0.0028740822287658205 1.13584077441839 >1k 0.003672438403422993 4.043906132573396 >5k 0.0 0.0 >10k+ 1.5967123493143448E-4 1.0608570263942139 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11389 1.0561124047073922 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4077 0.3780639453851996 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3483 0.32298178115689236 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3164 0.2934006188861348 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3140 0.29117507689711225 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2927 0.27142339174453745 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2663 0.2469424298652898 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2104 0.19510584770430708 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2023 0.1875946434913561 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1871 0.17349954422754682 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1830 0.1696975766629667 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1670 0.15486063006948328 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1553 0.14401111287299853 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 1528 0.14169283996776671 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1394 0.12926689719572437 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1337 0.12398123497179589 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1270 0.1177682635857747 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1081 0.10024212042222241 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.273091620927142E-5 0.0 10-11 0.0 0.0 0.0 9.273091620927142E-5 0.0 12-13 0.0 0.0 0.0 9.273091620927142E-5 0.0 14-15 0.0 0.0 0.0 9.273091620927142E-5 0.0 16-17 0.0 0.0 0.0 9.273091620927142E-5 0.0 18-19 0.0 0.0 0.0 1.8546183241854283E-4 0.0 20-21 0.0 0.0 0.0 1.8546183241854283E-4 0.0 22-23 0.0 0.0 0.0 1.8546183241854283E-4 0.0 24-25 0.0 0.0 0.0 2.781927486278143E-4 0.0 26-27 0.0 0.0 0.0 5.100200391509928E-4 0.0 28-29 0.0 0.0 0.0 0.0010200400783019856 0.0 30-31 0.0 0.0 0.0 0.0026428311119642356 0.0 32-33 0.0 0.0 0.0 0.004311987603731121 0.0 34-35 0.0 0.0 0.0 0.00625933684412582 9.273091620927142E-5 36-37 0.0 0.0 0.0 0.0084385133750437 9.273091620927142E-5 38-39 0.0 0.0 0.0 0.011081344487007934 9.273091620927142E-5 40-41 0.0 0.0 0.0 0.01395600288949535 9.273091620927142E-5 42-43 0.0 0.0 0.0 0.023414556342841036 9.273091620927142E-5 44-45 0.0 0.0 0.0 0.03305857162860526 9.273091620927142E-5 46-47 0.0 0.0 0.0 0.0436298960764622 9.273091620927142E-5 48-49 0.0 0.0 0.0 0.05489670239588868 9.273091620927142E-5 50-51 0.0 0.0 0.0 0.06843541616244231 9.273091620927142E-5 52-53 0.0 0.0 0.0 0.09393641811999195 9.273091620927142E-5 54-55 0.0 0.0 0.0 0.11299262140099722 9.273091620927142E-5 56-57 0.0 0.0 0.0 0.15574157377347136 9.273091620927142E-5 58-59 0.0 0.0 0.0 0.20934004334243023 9.273091620927142E-5 60-61 0.0 0.0 0.0 0.2632630711181216 1.8546183241854283E-4 62-63 0.0 0.0 0.0 0.3391633260354102 1.8546183241854283E-4 64-65 0.0 0.0 0.0 0.38627063146972007 1.8546183241854283E-4 66-67 0.0 0.0 0.0 0.490268353998418 1.8546183241854283E-4 68-69 0.0 0.0 0.0 0.6104939868637385 1.8546183241854283E-4 70-71 0.0 0.0 0.0 0.6954818715695357 2.3182729052317855E-4 72-73 0.0 0.0 0.0 0.8500643088903912 2.781927486278143E-4 74-75 0.0 0.0 0.0 0.9937508635566572 2.781927486278143E-4 76-77 0.0 0.0 0.0 1.0816597721230465 2.781927486278143E-4 78-79 0.0 0.0 0.0 1.1851474746125934 2.781927486278143E-4 80-81 1.8546183241854283E-4 0.0 0.0 1.3790941858642847 2.781927486278143E-4 82-83 1.8546183241854283E-4 0.0 0.0 1.5336766231851402 2.781927486278143E-4 84-85 1.8546183241854283E-4 0.0 0.0 1.7765388927372219 2.781927486278143E-4 86-87 1.8546183241854283E-4 0.0 0.0 2.124465290354408 2.781927486278143E-4 88-89 1.8546183241854283E-4 0.0 0.0 2.595909268362344 2.781927486278143E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 50 4.959054E-5 28.496662 94-95 GTCCTAC 200 6.366463E-10 26.398607 1 TAAGGTG 630 0.0 24.87804 5 GTAAGGT 580 0.0 22.92941 4 GAGTACT 4250 0.0 22.347212 12-13 GTACATG 4900 0.0 22.235561 1 GGTAAGG 610 0.0 21.802746 3 GTACTTT 4390 0.0 21.472286 14-15 TACATGG 5095 0.0 21.253641 2 ACATGGG 5020 0.0 20.626987 3 AGGTAAG 675 0.0 20.405018 2 GGACCGA 120 0.0047665406 19.789349 6 GTTCGAC 85 7.973668E-5 19.553812 10-11 GTGTAGC 395 0.0 19.442013 1 AGTACTT 4410 0.0 19.275106 12-13 AAGGTGT 850 0.0 18.997774 6 AAGGTAA 665 0.0 18.765928 1 TACCTGG 820 0.0 18.534414 2 ACTTTTT 5290 0.0 18.312887 16-17 CCGACCA 130 0.0075679435 18.267092 9 >>END_MODULE