FastQCFastQC Report
Thu 26 May 2016
SRR936685_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936685_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125067
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63110.5609443704241615No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT61520.5468118787592205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49550.4404182150929678No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43090.3829994124794346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37030.32913595368098075No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36320.32282521840921474No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27070.24060789268550228No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22670.20149911071962825No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20600.18310020647659206No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT19340.17190087345909177No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17020.15127987933163092No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC15870.14105826586327747No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG13230.11759299668375306No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA12320.1095045895044473No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT11710.10408269018645111No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA11610.10319385423268126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG50000.025.6605931
TACATGG50950.024.8101652
ACATGGG50450.024.2082943
GAGTACT46450.023.7811512-13
AGTACTT47750.023.03420612-13
GTACTTT48400.022.9211914-15
GTGTAGC5750.021.4871311
CATGGGA26000.021.0192264
AGAGTAC79450.020.7809910-11
ACTTTTT58550.019.39310516-17
TACTTTT56650.019.24768314-15
CCGCACA1250.00601515519.008694
TACCTGG7900.018.6477682
CCCTATA2302.048797E-618.5954592
CATGGGG24200.018.4588534
TGCGTAA1555.5988494E-918.38446860-61
ATGGGGA12850.018.120325
ATGGGAG11350.018.0030275
GTACCTG9150.017.657561
GTATCAA138450.017.4015481
Produced by FastQC (version 0.11.5)