##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936685_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1125067 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.4949838542949 31.0 31.0 34.0 30.0 34.0 2 31.53062528720512 33.0 31.0 34.0 30.0 34.0 3 31.5777398146066 33.0 31.0 34.0 28.0 34.0 4 35.19529236925445 37.0 35.0 37.0 33.0 37.0 5 35.165548362897496 37.0 35.0 37.0 33.0 37.0 6 35.29960349028102 37.0 35.0 37.0 33.0 37.0 7 35.17001565240115 37.0 35.0 37.0 32.0 37.0 8 35.11668993935472 37.0 35.0 37.0 32.0 37.0 9 36.621546983424096 39.0 37.0 39.0 32.0 39.0 10-11 36.522819529859106 39.0 37.0 39.0 32.0 39.0 12-13 36.432628456794134 39.0 37.0 39.0 32.0 39.0 14-15 37.48522621319441 40.0 37.0 41.0 32.0 41.0 16-17 37.38163371603647 40.0 37.0 41.0 32.0 41.0 18-19 37.35640010772692 40.0 37.0 41.0 32.0 41.0 20-21 37.27900693914229 40.0 37.0 41.0 31.5 41.0 22-23 37.14507980413611 39.0 36.5 41.0 31.0 41.0 24-25 37.271600269139526 40.0 37.0 41.0 31.0 41.0 26-27 37.24899761525313 40.0 37.0 41.0 31.0 41.0 28-29 37.162864078317114 39.5 36.5 41.0 31.0 41.0 30-31 36.96907428624251 39.0 36.0 41.0 30.5 41.0 32-33 36.77294818886342 39.0 36.0 41.0 30.0 41.0 34-35 36.53420996260667 39.0 35.0 41.0 30.0 41.0 36-37 36.331961118760034 39.0 35.0 40.5 30.0 41.0 38-39 36.11719524259444 39.0 35.0 40.0 29.5 41.0 40-41 35.93849477408901 38.5 35.0 40.0 29.0 41.0 42-43 35.69594299717261 38.0 35.0 40.0 28.0 41.0 44-45 35.3900754355074 38.0 35.0 40.0 27.0 41.0 46-47 35.04858155114317 38.0 34.0 40.0 25.5 41.0 48-49 34.901770738987096 38.0 34.0 40.0 24.5 41.0 50-51 33.95969128949653 37.5 33.0 39.5 23.5 40.5 52-53 34.10256455837741 38.0 33.5 39.5 24.0 40.5 54-55 34.508179068446594 38.0 34.0 40.0 24.0 41.0 56-57 34.37089879980481 38.0 34.0 40.0 23.0 41.0 58-59 34.096412035905416 38.0 33.5 40.0 22.5 41.0 60-61 33.74950914034453 37.5 33.0 40.0 22.0 41.0 62-63 33.442283437341956 37.0 33.0 40.0 21.5 41.0 64-65 33.09831058950267 36.5 32.5 39.5 20.0 41.0 66-67 32.67499491141416 36.0 32.0 39.0 18.5 41.0 68-69 32.25530035100132 35.5 31.5 39.0 17.5 40.5 70-71 31.789353878480128 35.0 31.0 38.0 13.5 40.0 72-73 31.40357729806314 35.0 31.0 37.5 11.5 40.0 74-75 30.992212463791045 35.0 31.0 37.0 8.0 39.0 76-77 30.567202219956677 35.0 30.0 36.5 7.0 39.0 78-79 30.09520632993413 34.0 30.0 36.0 3.5 38.5 80-81 29.602561447451574 34.0 29.0 35.5 2.0 37.0 82-83 29.134310667720236 34.0 29.0 35.0 2.0 37.0 84-85 28.80551869355336 34.0 29.0 35.0 2.0 36.5 86-87 28.51720386430319 34.0 29.0 35.0 2.0 36.0 88-89 28.09547431397419 34.0 27.5 35.0 2.0 36.0 90-91 27.671162250781506 34.0 26.5 35.0 2.0 35.0 92-93 27.299787479323456 33.0 26.0 35.0 2.0 35.0 94-95 26.84956895900422 33.0 25.0 35.0 2.0 35.0 96-97 26.287998403650626 33.0 24.5 35.0 2.0 35.0 98-99 25.589520001919887 33.0 22.0 35.0 2.0 35.0 100-101 23.125437862811726 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3839130165840636 1101 2 0.47017422818432664 1101 3 0.5765168383270343 1101 4 0.464413327050444 1101 5 0.4561533803906599 1101 6 0.4348419930654046 1101 7 0.431890368686247 1101 8 0.5914173808855097 1101 9 0.8834349075440784 1101 10-11 0.5992344441328683 1101 12-13 0.8410988458479167 1101 14-15 0.9936890342664313 1101 16-17 1.015415389423012 1101 18-19 1.108005590012091 1101 20-21 1.0799637513254083 1101 22-23 1.0067277062918407 1101 24-25 0.9053473479355958 1101 26-27 0.8480961827729914 1101 28-29 0.9032458953176956 1101 30-31 0.8083348313316421 1101 32-33 0.7045021001539382 1101 34-35 0.7742158656511151 1101 36-37 0.6045052090280834 1101 38-39 0.6494795104922559 1101 40-41 0.7889872995938063 1101 42-43 0.6332921626017836 1101 44-45 0.853979737850338 1101 46-47 0.5935280493948838 1101 48-49 0.7838548338880074 1101 50-51 0.7544205942874029 1101 52-53 0.82118781885098 1101 54-55 0.9490040497136008 1101 56-57 0.8042465052592647 1101 58-59 0.7549836364426028 1101 60-61 0.5950449317684345 1101 62-63 0.8051671549110111 1101 64-65 0.7922420248041746 1101 66-67 0.6822404594072253 1101 68-69 0.7531160500002088 1101 70-71 0.83971506619962 1101 72-73 0.693008782346098 1101 74-75 0.5975582130197736 1101 76-77 0.7348465578673462 1101 78-79 0.44352106434226 1101 80-81 0.7038139907809722 1101 82-83 0.9091701619448891 1101 84-85 1.012330718893132 1101 86-87 0.7522281378207545 1101 88-89 0.7181868567583827 1101 90-91 0.719718861120402 1101 92-93 0.6385278743307943 1101 94-95 0.9882610524959894 1101 96-97 0.8494097790227642 1101 98-99 0.7957014869074506 1101 100-101 1.4622412452434652 1102 1 0.22523649220923048 1102 2 0.34797297512279 1102 3 0.21738912457457715 1102 4 0.35248575127154425 1102 5 0.4103753017318539 1102 6 0.30733787715175964 1102 7 0.3322223179888937 1102 8 0.29308178598855505 1102 9 0.4325686576394787 1102 10-11 0.37293650650047994 1102 12-13 0.4408808833168578 1102 14-15 0.5180316527309046 1102 16-17 0.5792122986964827 1102 18-19 0.5391112418727104 1102 20-21 0.6254206846451282 1102 22-23 0.4515600912221984 1102 24-25 0.2805521524284629 1102 26-27 0.33512300138237805 1102 28-29 0.28768734526907735 1102 30-31 0.18803889193691958 1102 32-33 0.1702233043696637 1102 34-35 0.098410846466642 1102 36-37 0.21904258628168094 1102 38-39 0.1924436026768248 1102 40-41 0.16313921323346392 1102 42-43 0.25176604823240467 1102 44-45 0.09318271260771382 1102 46-47 0.25503194129974105 1102 48-49 0.46237372942557187 1102 50-51 0.5415039602473897 1102 52-53 0.4888137915794033 1102 54-55 0.4562350235868422 1102 56-57 0.4757963487009107 1102 58-59 0.6894821293392397 1102 60-61 0.4743195596016321 1102 62-63 0.6827874877722095 1102 64-65 0.531833050179614 1102 66-67 0.7227984395219877 1102 68-69 0.5329270599329163 1102 70-71 0.3752343354155592 1102 72-73 0.559670773903143 1102 74-75 0.3872047945564958 1102 76-77 0.5656376557978433 1102 78-79 0.2778977896311652 1102 80-81 0.27284908780353945 1102 82-83 0.39155028503077816 1102 84-85 0.36545702912151157 1102 86-87 0.4432342841857171 1102 88-89 0.45277113709051164 1102 90-91 0.42299469225742925 1102 92-93 0.34624745497842113 1102 94-95 0.6056612322977308 1102 96-97 0.37050922603824077 1102 98-99 0.6362508436751462 1102 100-101 0.7020396769837269 1103 1 0.2750471546258204 1103 2 0.27537760319366456 1103 3 0.17635850854467705 1103 4 0.36369518839475035 1103 5 0.37532441088731616 1103 6 0.22167739673567155 1103 7 0.29803455380284305 1103 8 0.41667703970906445 1103 9 0.5350381985416988 1103 10-11 0.508127922924956 1103 12-13 0.5559946692940514 1103 14-15 0.4337188523689335 1103 16-17 0.6088062317414966 1103 18-19 0.5642377047042473 1103 20-21 0.6882694509956266 1103 22-23 0.5765972861726496 1103 24-25 0.677046757632084 1103 26-27 0.39634385175065745 1103 28-29 0.19777084538524292 1103 30-31 0.45869839407486523 1103 32-33 0.5752437055452262 1103 34-35 0.3756100361262469 1103 36-37 0.6213406227959126 1103 38-39 0.6266075213329998 1103 40-41 0.43679163508330276 1103 42-43 0.48852719347002704 1103 44-45 0.5443248523465982 1103 46-47 0.38898879108105433 1103 48-49 0.48578361981765994 1103 50-51 0.426832437516687 1103 52-53 0.36194385365929804 1103 54-55 0.407702746522169 1103 56-57 0.3587958106479334 1103 58-59 0.24499992381115732 1103 60-61 0.26102689954105784 1103 62-63 0.28885984366979045 1103 64-65 0.2759200720971151 1103 66-67 0.21973826318735235 1103 68-69 0.2055355774746701 1103 70-71 0.35568966275150515 1103 72-73 0.5405449265716058 1103 74-75 0.3793887597624028 1103 76-77 0.35531906321400797 1103 78-79 0.3335974826963941 1103 80-81 0.23036073542774815 1103 82-83 0.22383621535291454 1103 84-85 0.40603519150753087 1103 86-87 0.3802887090600713 1103 88-89 0.5518370852662251 1103 90-91 0.6997592614685679 1103 92-93 0.4711261892495422 1103 94-95 0.38128091036270106 1103 96-97 0.47405232530494246 1103 98-99 0.818835760256551 1103 100-101 1.187187172795472 1104 1 0.1706078190690441 1104 2 0.3311412919544736 1104 3 0.26299301080589643 1104 4 0.4956761056199781 1104 5 0.565852101418244 1104 6 0.4105979463368499 1104 7 0.39296110454812805 1104 8 0.32902147947635996 1104 9 0.45028660920991825 1104 10-11 0.41469707099994224 1104 12-13 0.379557254966862 1104 14-15 0.5302884321809174 1104 16-17 0.22986150486910617 1104 18-19 0.39375448308286565 1104 20-21 0.5276964820483059 1104 22-23 0.4861489273128612 1104 24-25 0.5914114566574327 1104 26-27 0.11898899632857507 1104 28-29 0.44497561823762055 1104 30-31 0.6042404398605683 1104 32-33 0.27351621793068404 1104 34-35 0.08818167748232497 1104 36-37 0.35998998760287293 1104 38-39 0.4262362275251874 1104 40-41 0.44108606891421687 1104 42-43 0.2999317957862999 1104 44-45 0.34225035612969634 1104 46-47 0.3869142948641482 1104 48-49 0.142723522029236 1104 50-51 -0.01584152550503859 1104 52-53 0.16314273330182516 1104 54-55 0.4186475489485275 1104 56-57 0.2841125976612844 1104 58-59 0.1875413975453597 1104 60-61 0.4207768698591394 1104 62-63 0.4458974263744082 1104 64-65 0.3508947075492017 1104 66-67 0.46219473870247185 1104 68-69 0.13963605346469166 1104 70-71 0.2611573418414004 1104 72-73 0.13514794441313427 1104 74-75 0.02599638639625823 1104 76-77 0.15238918809393098 1104 78-79 0.10168294503273856 1104 80-81 0.21733388138722987 1104 82-83 0.21109268870934628 1104 84-85 0.08386603695648631 1104 86-87 0.26479555748801076 1104 88-89 0.4493939935976705 1104 90-91 0.5151234316161144 1104 92-93 0.4968871256870635 1104 94-95 0.4162273210093339 1104 96-97 0.544499658789384 1104 98-99 0.6676913874084924 1104 100-101 1.1874154537267287 1105 1 0.2886717775824401 1105 2 0.32284397129760123 1105 3 0.3576631346895063 1105 4 0.3638408996274691 1105 5 0.42502813138079176 1105 6 0.44943401663359595 1105 7 0.37037395387108774 1105 8 0.4793527960998105 1105 9 0.6818049036495637 1105 10-11 0.46370312112959056 1105 12-13 0.4904762984701776 1105 14-15 0.1834719527801667 1105 16-17 0.46348293961413844 1105 18-19 0.44212671010676985 1105 20-21 0.37117098507336976 1105 22-23 0.34434541247564 1105 24-25 0.44033107636357016 1105 26-27 0.5379172590770693 1105 28-29 0.4072055231641514 1105 30-31 0.3569629982011051 1105 32-33 0.4502664340067426 1105 34-35 0.3337305106716144 1105 36-37 0.32570591961093953 1105 38-39 0.34623046549695147 1105 40-41 0.03961675171456136 1105 42-43 0.38051376063792475 1105 44-45 0.09361883783525116 1105 46-47 0.2667183859041984 1105 48-49 0.2325247320551611 1105 50-51 0.2074946888424094 1105 52-53 0.2921833556008693 1105 54-55 0.3280108448286754 1105 56-57 0.525628011086809 1105 58-59 0.5882616510746033 1105 60-61 0.16704595657765964 1105 62-63 0.43566982625917205 1105 64-65 0.6448157677623954 1105 66-67 0.63119502680388 1105 68-69 0.6570949990135304 1105 70-71 0.7044877941606167 1105 72-73 0.6286656626499507 1105 74-75 0.7173821541865202 1105 76-77 0.44596452661308916 1105 78-79 0.48114131437874974 1105 80-81 0.21868795802220475 1105 82-83 0.3939506547133824 1105 84-85 0.5836067456859553 1105 86-87 0.7285812100377065 1105 88-89 0.4956342701750245 1105 90-91 0.5235648029788358 1105 92-93 0.5471896321693457 1105 94-95 0.5635911930923037 1105 96-97 0.6499447754704555 1105 98-99 0.8866484602981473 1105 100-101 1.289230492620419 1106 1 0.3674716713419883 1106 2 0.3409884905962386 1106 3 0.3667192528335832 1106 4 0.4518018313151515 1106 5 0.5030502980697165 1106 6 0.36592373816328205 1106 7 0.49335534858519026 1106 8 0.4031069286920044 1106 9 0.5038675532336256 1106 10-11 0.4199974277272176 1106 12-13 0.7007278418181855 1106 14-15 0.7426896327175925 1106 16-17 0.7812178315320892 1106 18-19 0.8061095825023088 1106 20-21 0.5080850667757986 1106 22-23 0.6463878264817708 1106 24-25 0.5407371085908252 1106 26-27 0.19959314352149704 1106 28-29 0.5814457351754925 1106 30-31 0.5061919478440089 1106 32-33 0.30162995097659717 1106 34-35 0.38401826968774344 1106 36-37 0.2606373108766249 1106 38-39 0.28377201153032416 1106 40-41 0.30924005300455804 1106 42-43 0.05819724919873437 1106 44-45 0.48585374662840763 1106 46-47 0.5070881437669428 1106 48-49 0.4148463354055494 1106 50-51 0.024861627811382903 1106 52-53 0.33785611152811157 1106 54-55 0.6205415100201108 1106 56-57 0.49703076658849454 1106 58-59 0.46494421956960963 1106 60-61 0.6584370314991972 1106 62-63 0.728363740764955 1106 64-65 0.45523965836407854 1106 66-67 0.46341435170743495 1106 68-69 0.6336527643248431 1106 70-71 0.7076031046718221 1106 72-73 0.862203895272522 1106 74-75 0.5773810096070591 1106 76-77 0.6427683618347331 1106 78-79 0.8449095781250726 1106 80-81 0.8283269312463837 1106 82-83 1.0509873238110536 1106 84-85 1.1219252117146894 1106 86-87 1.0450706380854804 1106 88-89 1.1849389088034066 1106 90-91 1.2752284785756665 1106 92-93 1.1982873278961037 1106 94-95 1.1395252359670813 1106 96-97 1.2119420959805858 1106 98-99 1.4518109832447657 1106 100-101 1.6337947673605804 1107 1 0.2713765949413087 1107 2 0.3246635591609497 1107 3 0.19240816494015434 1107 4 0.13099733869402286 1107 5 0.29936739353791353 1107 6 0.34466214746196044 1107 7 0.3326266036459131 1107 8 0.5091667432407831 1107 9 0.28413397611464575 1107 10-11 0.44385141754045065 1107 12-13 0.31177002544101384 1107 14-15 0.4147870599083987 1107 16-17 0.4054244398643334 1107 18-19 0.5777042018048988 1107 20-21 0.6105468664362448 1107 22-23 0.7465380158509802 1107 24-25 0.6877267912341551 1107 26-27 0.40330869791616664 1107 28-29 0.4882499665977207 1107 30-31 0.5215765632286278 1107 32-33 0.33783864890006754 1107 34-35 0.5409269806711592 1107 36-37 0.5504113738255612 1107 38-39 0.5742839613801394 1107 40-41 0.4595730816058392 1107 42-43 0.4866548696767268 1107 44-45 0.4627768235514864 1107 46-47 0.43537598495801433 1107 48-49 0.5830910787776347 1107 50-51 0.3407294611664824 1107 52-53 0.33560327099003473 1107 54-55 0.7317273530395667 1107 56-57 0.49874814937548706 1107 58-59 0.7179937167617254 1107 60-61 0.6809066671267274 1107 62-63 0.5968762130125711 1107 64-65 0.5960781068425902 1107 66-67 0.6460838044957313 1107 68-69 0.740150087036092 1107 70-71 0.6775260509954428 1107 72-73 0.7353526480477584 1107 74-75 0.6880640447383115 1107 76-77 0.6424026833705838 1107 78-79 0.83354089587354 1107 80-81 0.8718426684802871 1107 82-83 1.2001645472667164 1107 84-85 0.9832155342953364 1107 86-87 0.8916162825937697 1107 88-89 0.8447403976371568 1107 90-91 0.8735306857063954 1107 92-93 0.8664863614601614 1107 94-95 0.9017297547081 1107 96-97 0.7892569712581086 1107 98-99 0.8953006005883744 1107 100-101 1.5087000827582528 1108 1 -0.18687095230145445 1108 2 -0.04461628380310145 1108 3 0.04853587561331807 1108 4 0.19728256861561277 1108 5 0.27710353299654145 1108 6 0.03579578787790183 1108 7 -0.12751846128383448 1108 8 -0.01832532874889381 1108 9 -0.01614894139314771 1108 10-11 -0.14467499490940128 1108 12-13 0.2128957471408981 1108 14-15 -0.05228706745280931 1108 16-17 0.1021275772373258 1108 18-19 0.02827022270585644 1108 20-21 -0.08332486523453753 1108 22-23 0.1645034634814948 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-1.7147716696879414 2316 64-65 -2.1728184719224046 2316 66-67 -2.3097811697902912 2316 68-69 -2.8990546034843874 2316 70-71 -3.186153873868694 2316 72-73 -2.6374251905195507 2316 74-75 -2.1065752301641254 2316 76-77 -2.287926127221386 2316 78-79 -2.8497582433466526 2316 80-81 -2.705331621194002 2316 82-83 -2.8376609806903907 2316 84-85 -2.3168615164396087 2316 86-87 -2.758940397568171 2316 88-89 -3.286016979172853 2316 90-91 -3.0891115618042697 2316 92-93 -3.9225733460205987 2316 94-95 -4.213308816251523 2316 96-97 -5.405843240289471 2316 98-99 -6.089881494462144 2316 100-101 -6.092859624722507 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2565.0 3 1101.0 4 1045.0 5 1057.0 6 1206.0 7 1347.0 8 1801.0 9 2190.0 10 2686.0 11 3422.0 12 4291.0 13 5498.0 14 6506.0 15 8063.0 16 10103.0 17 12940.0 18 10740.0 19 7803.0 20 7725.0 21 8004.0 22 8976.0 23 9878.0 24 11410.0 25 13123.0 26 15090.0 27 17720.0 28 20763.0 29 25180.0 30 30338.0 31 37598.0 32 46980.0 33 60319.0 34 80686.0 35 111369.0 36 155535.0 37 200052.0 38 155569.0 39 24362.0 40 26.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.98497809620598 17.736693900813645 14.598765349771625 22.679562653208748 2 15.5261700347701 19.601766331767745 39.595685342593505 25.27637829086865 3 20.232249873980173 23.563885162376657 29.838027849715022 26.365837113928148 4 12.423221909690202 15.913640377516547 37.815860639373525 33.84727707341973 5 13.577155155164059 36.881511487787414 34.23036048574643 15.310972871302098 6 31.680561298670117 35.51227840840881 17.30581973882538 15.501340554095695 7 28.23611435950018 32.798008695505246 20.446620642798642 18.51925630219593 8 26.598184377537077 32.152559238039686 19.956285630190305 21.29297075423294 9 25.974845899120393 16.986576417319053 21.102661596958175 35.93591608660238 10-11 25.234739018055684 25.44919112471526 26.975878473285974 22.340191383943083 12-13 26.962897094457905 23.217882311095302 27.34112887090679 22.478091723540004 14-15 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30.122939890127476 22.371237577115224 40-41 22.024145754493716 25.71333971827731 30.21059204430055 22.051922482928425 42-43 21.5994001993706 25.900000933299733 29.260679948855174 23.23991891847449 44-45 21.88456494194204 25.513059763679408 29.445277254709723 23.15709803966883 46-47 22.76497773677261 26.09439572858906 28.96943855958097 22.171187975057364 48-49 22.516424730669083 25.984872759723622 28.637806190178004 22.86089631942929 50-51 21.7670032700391 26.476028453517714 29.393731728800965 22.36323654764222 52-53 22.5140974770316 26.697119350342746 28.1465106522172 22.642272520408454 54-55 21.709311679711284 26.024480326404355 28.84945132601768 23.416756667866682 56-57 22.495083867618245 26.124176347802585 28.136190201872573 23.244549582706593 58-59 23.055445056080647 25.776917486893776 28.160105960971336 23.00753149605424 60-61 22.377010999717317 25.910939091754393 29.45695673894917 22.255093169579123 62-63 22.009126210053942 26.291209389498594 29.127072275014743 22.57259212543272 64-65 21.64467015002251 26.872459312332758 28.68711082786809 22.79575970977664 66-67 21.881768148805605 26.41645284890592 28.085098061351065 23.61668094093741 68-69 21.8720205036024 26.14040398248517 28.80932156491985 23.178253948992577 70-71 22.420605466927057 26.121905842132726 28.192914915158717 23.2645737757815 72-73 22.168164620729407 25.829563448943123 29.027198721461428 22.97507320886604 74-75 21.75943744650062 27.178797045011926 28.187718719694793 22.87404678879266 76-77 21.929810915882168 26.321684338903378 28.27932697200961 23.469177773204837 78-79 22.17107108205995 26.32178017819738 28.469802135755167 23.03734660398751 80-81 22.671354344917706 26.444550129563044 27.913879468591517 22.970216056927732 82-83 22.335521944407528 26.236665998512876 28.678464395921566 22.74934766115803 84-85 22.08984202237672 26.572697665384005 28.084999704411466 23.252460607827807 86-87 22.039425878149103 26.867818199191575 28.125437491250178 22.96731843140915 88-89 21.91018626749452 26.74985222156543 27.414921844097083 23.925039666842967 90-91 21.945669482760994 26.2306542586075 28.02142003508377 23.802256223547726 92-93 22.481018824934026 26.310946785146122 28.42204549970241 22.785988890217443 94-95 21.92991969335199 25.933206985629432 28.51806055089258 23.618812770126 96-97 21.25984146977801 26.057660768081327 29.220131578128626 23.46236618401204 98-99 21.467781591296937 26.7092046182453 27.937334609412613 23.885679181045155 100-101 21.26925723919256 26.25426580191741 28.25788117463157 24.218595784258458 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 64.0 1 54.5 2 80.0 3 106.0 4 92.0 5 104.5 6 206.5 7 506.0 8 928.5 9 1126.0 10 1038.5 11 958.5 12 1006.0 13 1140.5 14 1230.5 15 1323.0 16 1414.5 17 1489.5 18 1637.5 19 1758.0 20 1911.5 21 2231.0 22 2698.5 23 3218.5 24 3875.0 25 4781.5 26 6046.5 27 7572.0 28 9080.0 29 10901.5 30 12999.5 31 15701.0 32 18956.5 33 20870.5 34 22189.5 35 24035.5 36 26278.5 37 28330.0 38 29334.0 39 29852.0 40 30338.0 41 31418.5 42 32374.0 43 33309.0 44 34771.5 45 36639.0 46 38454.5 47 44992.5 48 58973.5 49 57747.5 50 43784.5 51 37654.5 52 35925.5 53 36073.5 54 35939.0 55 33681.0 56 30928.5 57 28503.5 58 26726.0 59 24044.5 60 20110.5 61 16990.5 62 14179.5 63 10960.0 64 8084.5 65 6213.0 66 4612.5 67 3564.5 68 2712.5 69 1896.5 70 1429.0 71 1106.0 72 897.0 73 789.0 74 627.0 75 479.5 76 363.5 77 225.5 78 142.0 79 95.5 80 65.0 81 49.0 82 30.5 83 17.0 84 12.5 85 9.5 86 5.0 87 5.5 88 3.5 89 2.5 90 2.5 91 1.5 92 2.0 93 2.0 94 1.5 95 1.5 96 2.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.033420231861746905 2 0.02222089884424661 3 0.02168759727198469 4 0.0497748134111124 5 0.0543967603707157 6 0.04728607274055678 7 0.05137471812789816 8 0.0439084961162313 9 0.042575242185576505 10-11 0.050974741948701725 12-13 0.04373072892547733 14-15 0.03964208353813595 16-17 0.045730609821459524 18-19 0.05159692711634063 20-21 0.01159930919669673 22-23 0.0209765285089688 24-25 0.005866317294881105 26-27 0.01146598380363125 28-29 0.006177409878700558 30-31 0.010043846277599468 32-33 0.003910878196587403 34-35 0.009510544705337548 36-37 0.009777195491468509 38-39 0.011288216612877277 40-41 0.0021776480867361676 42-43 0.0023554152774901403 44-45 0.0024442988728671272 46-47 4.888597745734254E-4 48-49 9.332777514583576E-4 50-51 3.5553438150794575E-4 52-53 0.003821994601210417 54-55 0.007288454820912888 56-57 0.017687835480020304 58-59 0.011066007624434812 60-61 0.01146598380363125 62-63 0.0058218754971926115 64-65 2.2220898844246608E-4 66-67 0.00733289661860138 68-69 0.017021208514692902 70-71 0.007466222011666861 72-73 0.0028887168497520595 74-75 0.0047997141503572675 76-77 0.008132848976994258 78-79 0.006977362237093435 80-81 0.01173263458976221 82-83 0.006799595046339462 84-85 0.01711009211006989 86-87 0.0039553199942758965 88-89 0.006399618867143023 90-91 0.004044203589652883 92-93 0.017998928063839754 94-95 0.01146598380363125 96-97 0.009599428300714535 98-99 0.02377636176334387 100-101 0.03777552803521924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1125067.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.549889245180275 #Duplication Level Percentage of deduplicated Percentage of total 1 82.45539050782088 49.92664762916644 2 10.519479433093124 12.73906629181481 3 3.008620526539879 5.465149189882969 4 1.3274001433254983 3.214957266495814 5 0.6759516313869536 2.0464398207789483 6 0.4032341019269661 1.4649468126934522 7 0.27741716805459143 1.175830516029195 8 0.2077709662485389 1.0064407195770497 9 0.1526936236350676 0.8321023799593726 >10 0.866405199617519 9.792408651668774 >50 0.06631486431601695 2.770194042312505 >100 0.03343092592460988 3.875390778171117 >500 0.0030927267579382457 1.2829385461293668 >1k 0.002503635946902389 3.296118543169513 >5k 2.9454540551792816E-4 1.1113688121505731 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6311 0.5609443704241615 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6152 0.5468118787592205 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4955 0.4404182150929678 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4309 0.3829994124794346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3703 0.32913595368098075 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3632 0.32282521840921474 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2707 0.24060789268550228 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2267 0.20149911071962825 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2060 0.18310020647659206 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1934 0.17190087345909177 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1702 0.15127987933163092 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1587 0.14105826586327747 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1323 0.11759299668375306 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1232 0.1095045895044473 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1171 0.10408269018645111 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1161 0.10319385423268126 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.444179768849322E-5 0.0 16-17 0.0 0.0 0.0 1.3332539306547966E-4 0.0 18-19 0.0 0.0 0.0 1.7776719075397287E-4 0.0 20-21 0.0 0.0 0.0 1.7776719075397287E-4 0.0 22-23 0.0 0.0 0.0 2.666507861309593E-4 0.0 24-25 4.444179768849322E-5 0.0 0.0 2.666507861309593E-4 0.0 26-27 8.888359537698644E-5 0.0 0.0 5.333015722619186E-4 0.0 28-29 8.888359537698644E-5 0.0 0.0 0.0011554867399008236 0.0 30-31 8.888359537698644E-5 0.0 0.0 0.0026220660636211 0.0 32-33 8.888359537698644E-5 0.0 0.0 0.004310854375783843 0.0 34-35 8.888359537698644E-5 0.0 0.0 0.006977362237093435 0.0 36-37 8.888359537698644E-5 0.0 0.0 0.008932801335387135 0.0 38-39 8.888359537698644E-5 0.0 0.0 0.012354819757401114 8.888359537698644E-5 40-41 8.888359537698644E-5 0.0 0.0 0.015599070988661121 8.888359537698644E-5 42-43 8.888359537698644E-5 0.0 0.0 0.024354105133294286 8.888359537698644E-5 44-45 1.7776719075397287E-4 0.0 0.0 0.034353509613205255 8.888359537698644E-5 46-47 2.666507861309593E-4 0.0 0.0 0.04404182150929678 8.888359537698644E-5 48-49 2.666507861309593E-4 0.0 0.0 0.05604110688518995 8.888359537698644E-5 50-51 2.666507861309593E-4 0.0 0.0 0.07279566461375189 8.888359537698644E-5 52-53 2.666507861309593E-4 0.0 0.0 0.10430489917489358 8.888359537698644E-5 54-55 2.666507861309593E-4 0.0 0.0 0.12465924251622348 8.888359537698644E-5 56-57 2.666507861309593E-4 0.0 0.0 0.16603455616421064 8.888359537698644E-5 58-59 2.666507861309593E-4 0.0 0.0 0.22456440371995623 8.888359537698644E-5 60-61 2.666507861309593E-4 0.0 0.0 0.28309425127570176 8.888359537698644E-5 62-63 2.666507861309593E-4 0.0 0.0 0.3589119581322713 8.888359537698644E-5 64-65 2.666507861309593E-4 0.0 0.0 0.4106422106416774 8.888359537698644E-5 66-67 2.666507861309593E-4 0.0 0.0 0.5122806019552613 8.888359537698644E-5 68-69 2.666507861309593E-4 0.0 0.0 0.6327178736910779 8.888359537698644E-5 70-71 2.666507861309593E-4 0.0 0.0 0.7204459823281635 8.888359537698644E-5 72-73 3.1109258381945254E-4 0.0 0.0 0.8669261475094372 8.888359537698644E-5 74-75 3.5553438150794575E-4 0.0 0.0 1.007184461014322 8.888359537698644E-5 76-77 3.5553438150794575E-4 0.0 0.0 1.0986901224549293 8.888359537698644E-5 78-79 3.5553438150794575E-4 0.0 0.0 1.2045949263466087 8.888359537698644E-5 80-81 4.4441797688493217E-4 0.0 0.0 1.3927614977596892 8.888359537698644E-5 82-83 4.4441797688493217E-4 0.0 0.0 1.547818929894842 8.888359537698644E-5 84-85 4.4441797688493217E-4 0.0 0.0 1.7917599574069811 8.888359537698644E-5 86-87 4.4441797688493217E-4 8.888359537698644E-5 0.0 2.1338284742153135 8.888359537698644E-5 88-89 4.4441797688493217E-4 8.888359537698644E-5 0.0 2.6068225270139465 8.888359537698644E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5000 0.0 25.660593 1 TACATGG 5095 0.0 24.810165 2 ACATGGG 5045 0.0 24.208294 3 GAGTACT 4645 0.0 23.78115 12-13 AGTACTT 4775 0.0 23.034206 12-13 GTACTTT 4840 0.0 22.92119 14-15 GTGTAGC 575 0.0 21.487131 1 CATGGGA 2600 0.0 21.019226 4 AGAGTAC 7945 0.0 20.78099 10-11 ACTTTTT 5855 0.0 19.393105 16-17 TACTTTT 5665 0.0 19.247683 14-15 CCGCACA 125 0.006015155 19.00869 4 TACCTGG 790 0.0 18.647768 2 CCCTATA 230 2.048797E-6 18.595459 2 CATGGGG 2420 0.0 18.458853 4 TGCGTAA 155 5.5988494E-9 18.384468 60-61 ATGGGGA 1285 0.0 18.12032 5 ATGGGAG 1135 0.0 18.003027 5 GTACCTG 915 0.0 17.65756 1 GTATCAA 13845 0.0 17.401548 1 >>END_MODULE