FastQCFastQC Report
Thu 26 May 2016
SRR936685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936685_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125067
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT117721.0463376847778845No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43960.39073228527723236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38650.3435350961320526No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT32940.29278256317179335No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT32920.2926047959810394No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC30060.2671840877032212No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27770.2468297443618913No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23300.2070987772283784No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22880.20336566622254498No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20530.18247802130895316No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT19430.17270082581748464No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA18460.16407911706591696No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA15870.14105826586327747No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA15730.13981389552799967No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC14690.1305700016087931No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14230.1264813562214517No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC13740.12212606004797935No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA11620.10328273782805825No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA11480.10203836749278043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAG5100.029.802192
TAAGGTG5900.027.368865
AAGGTAA5400.027.2754231
GTAAGGT5750.027.2568664
CCTATAC2104.3655746E-1127.1400763
GGTAAGG6550.024.6539633
GAGTACT49250.024.11134712-13
GTACATG53300.023.9788631
GTACTTT50050.023.63104814-15
ACGTTCG1852.5451482E-723.1047137
TACATGG55450.022.6136572
CCCCTAT2354.634785E-922.2396721
ACATGGG54250.022.1498053
AGTACTT49150.021.45444112-13
ACTTTTT58400.020.5351416-17
TCGCAAG1404.8564584E-420.3541535
GCGTCGA600.004764653319.79183844-45
TATACTG5050.019.7495755
TACACCG1507.7285484E-418.997215
TACTTTT55150.018.99112914-15
Produced by FastQC (version 0.11.5)