FastQCFastQC Report
Thu 26 May 2016
SRR936684_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936684_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138188
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT62350.5478005391025033No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT62230.5467462317297318No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48410.42532516596555225No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44500.3909723174027489No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37390.32850460556604005No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36980.3249023887090709No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT28090.24679578417625206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22440.1971554787082626No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC21400.1880181481442433No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20250.1779143691551835No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17030.14962378798581605No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC15730.13820212478079194No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG13390.1176431310117485No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT12790.11237159414789122No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA12510.10991154361142447No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA12020.1056064551726077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC700.0083694827.1575053
GTACATG49950.025.6906621
TACATGG50150.025.302782
ACATGGG49650.024.5046583
ATACCGT1252.2469876E-422.8133076
GAGTACT46550.022.66927312-13
AGTACTT48950.022.1404512-13
TACCTGG8700.021.8508662
ACGTATA1100.002868354621.602562
GTACTTT49450.020.9969314-15
TATTCCG1404.8326422E-420.3690245
CCTATAC2801.5643309E-920.368133
GTACCTG9400.020.2245641
GTGTAGC5250.019.916381
ATAGGAC2151.0732056E-619.8944533
GTATAGG3353.45608E-1119.8623331
AGAGTAC80700.019.58580810-11
CATGGGA25250.019.5757754
TAAGGTG5400.019.3631465
TACACCG1759.892259E-519.011094
Produced by FastQC (version 0.11.5)